pycom12g16180

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0017284
Physical Location & Seq
Reverse (-)
18574327 .. 18575275
949 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g16180.2

Sequence Viewer

Length: 294 bp
ATGTTCGCCAAGCGGCTTTTGCAGAAAGCCATAAACAACTCTCAGCATAATTTGCCATACGGGAGTTTGACGGCGGAAGACTTGGACCTGCGAGTTGCAGTTCACTATGGCATCCCATCCACGGCCTCCATTCTTGCTTTCGACCCGATTCAGCGCCTTTTGGCCATTGGAACATTGGATGGGAGGATTAAAGTGATCGGTGGTGATGGTATTGAAGGACTCTTTATATCCCGAAGCAACTGCCTTACAAATACATTGAGGTTTCTGTGTGCACCATTCATTCCCCTTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000137 GO:0000139 GO:0000902 GO:0000904 GO:0002791 GO:0003674 GO:0005096 GO:0005198 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005795 GO:0005802 GO:0005856 GO:0005886 GO:0005938 GO:0006810 GO:0006892 GO:0006893 GO:0006996 GO:0007010 GO:0007275 GO:0007399 GO:0007409 GO:0008047 GO:0008150 GO:0009653 GO:0009987 GO:0010008 GO:0012505 GO:0015629 GO:0016020 GO:0016043 GO:0016192 GO:0017016 GO:0017137 GO:0017157 GO:0019899 GO:0019900 GO:0019901 GO:0022008 GO:0022607 GO:0030029 GO:0030030 GO:0030036 GO:0030154 GO:0030182 GO:0030234 GO:0030695 GO:0030863 GO:0030864 GO:0030865 GO:0030866 GO:0031090 GO:0031175 GO:0031267 GO:0031410 GO:0031901 GO:0031982 GO:0031984 GO:0031985 GO:0032501 GO:0032502 GO:0032588 GO:0032879 GO:0032880 GO:0032989 GO:0032990 GO:0043085 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043547 GO:0043933 GO:0044085 GO:0044093 GO:0044422 GO:0044424 GO:0044430 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0048193 GO:0048468 GO:0048666 GO:0048667 GO:0048699 GO:0048731 GO:0048812 GO:0048856 GO:0048858 GO:0048869 GO:0050708 GO:0050789 GO:0050790 GO:0050794 GO:0051020 GO:0051046 GO:0051049 GO:0051179 GO:0051223 GO:0051234 GO:0051336 GO:0051345 GO:0060589 GO:0060627 GO:0061564 GO:0065003 GO:0065007 GO:0065009 GO:0070201 GO:0071840 GO:0071944 GO:0090087 GO:0097708 GO:0098588 GO:0098772 GO:0098791 GO:0098805 GO:0098876 GO:0099568 GO:0120036 GO:0120039 GO:1903530
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANAPC4_WD40 Synaptobrevin

Protein Analysis

98

Amino Acids

10.57

Weight (kDa)

8.77

Isoelectric Point (pI)

47.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017284)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08370 FvH4_4g08370 FvH4_4g08370 FvH4_4g08370 FvH4_4g08370 FvH4_4g08370
pyrus_communis pycom12g10530 pycom12g16180
rosa_chinensis RchiOBHm_Chr6g0296391
rosa_laevigata RLG00000027043
rosa_multiflora Rmu_co8421439.1_g000001 Rmu_sc0011275.1_g000003
rosa_samantha Rh2BG113800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 96
AciI CCGC 2 cut(s) 13, 74
AcoI YGGCCR 1 cut(s) 162
AfiI CCNNNNNNNGG 1 cut(s) 121
AgsI TTSAA 1 cut(s) 215
Alw21I GWGCWC 1 cut(s) 274
Alw44I GTGCAC 1 cut(s) 270
AoxI GGCC 2 cut(s) 123, 162
ApaLI GTGCAC 1 cut(s) 270
AspLEI GCGC 1 cut(s) 156
AspS9I GGNCC 1 cut(s) 85
AsuHPI GGTGA 1 cut(s) 215
AvaII GGWCC 1 cut(s) 85
BaeGI GKGCMC 1 cut(s) 274
BalI TGGCCA 1 cut(s) 164
BbsI GAAGAC 1 cut(s) 84
Bbv12I GWGCWC 1 cut(s) 274
BccI CCATC 3 cut(s) 124, 173, 200
BceAI ACGGC 2 cut(s) 87, 138
BcgI CGANNNNNNTGC 2 cut(s) 222, 256
BfoI RGCGCY 1 cut(s) 157
BfuAI ACCTGC 1 cut(s) 96
BisI GCNGC 1 cut(s) 14
BlsI GCNGC 1 cut(s) 15
Bme18I GGWCC 1 cut(s) 85
BmgT120I GGNCC 1 cut(s) 85
BmsI GCATC 1 cut(s) 120
BpiI GAAGAC 1 cut(s) 84
BsaJI CCNNGG 1 cut(s) 120
Bsc4I CCNNNNNNNGG 1 cut(s) 121
BseDI CCNNGG 1 cut(s) 120
BseGI GGATG 3 cut(s) 111, 116, 184
BseLI CCNNNNNNNGG 1 cut(s) 121
BseMII CTCAG 1 cut(s) 56
BseSI GKGCMC 1 cut(s) 274
BshFI GGCC 2 cut(s) 125, 164
BsiHKAI GWGCWC 1 cut(s) 274
BslI CCNNNNNNNGG 1 cut(s) 121
BsnI GGCC 2 cut(s) 125, 164
Bsp1286I GDGCHC 1 cut(s) 274
Bsp143I GATC 1 cut(s) 195
BspACI CCGC 2 cut(s) 13, 74
BspANI GGCC 2 cut(s) 125, 164
BspCNI CTCAG 1 cut(s) 55
BspMI ACCTGC 1 cut(s) 96
BssECI CCNNGG 1 cut(s) 120
BssMI GATC 1 cut(s) 195
BstAPI GCANNNNNTGC 1 cut(s) 52
BstDEI CTNAG 1 cut(s) 42
BstDSI CCRYGG 1 cut(s) 120
BstF5I GGATG 3 cut(s) 111, 116, 184
BstH2I RGCGCY 1 cut(s) 157
BstHHI GCGC 1 cut(s) 156
BstKTI GATC 1 cut(s) 198
BstMBI GATC 1 cut(s) 195
BstMWI GCNNNNNNNGC 2 cut(s) 19, 52
BstSLI GKGCMC 1 cut(s) 274
BstV2I GAAGAC 1 cut(s) 84
BsuRI GGCC 2 cut(s) 125, 164
BtgI CCRYGG 1 cut(s) 120
BtsCI GGATG 3 cut(s) 111, 116, 184
BveI ACCTGC 1 cut(s) 96
CfoI GCGC 1 cut(s) 156
Cfr13I GGNCC 1 cut(s) 85
CviJI RGCY 4 cut(s) 16, 29, 125, 164
CviKI_1 RGCY 4 cut(s) 16, 29, 125, 164
DdeI CTNAG 1 cut(s) 42
DpnI GATC 1 cut(s) 197
DpnII GATC 1 cut(s) 195
EaeI YGGCCR 1 cut(s) 162
EciI GGCGGA 1 cut(s) 89
Eco47I GGWCC 1 cut(s) 85
FaiI YATR 5 cut(s) 32, 48, 58, 108, 227
Fnu4HI GCNGC 1 cut(s) 14
FokI GGATG 3 cut(s) 98, 103, 191
Fsp4HI GCNGC 1 cut(s) 14
GlaI GCGC 1 cut(s) 155
GluI GCNGC 1 cut(s) 14
HaeII RGCGCY 1 cut(s) 157
HaeIII GGCC 2 cut(s) 125, 164
HhaI GCGC 1 cut(s) 156
Hin6I GCGC 1 cut(s) 154
HinP1I GCGC 1 cut(s) 154
HinfI GANTC 2 cut(s) 148, 219
HphI GGTGA 1 cut(s) 215
Hpy166II GTNNAC 2 cut(s) 103, 272
Hpy188III TCNNGA 1 cut(s) 231
Hpy8I GTNNAC 2 cut(s) 103, 272
HpyAV CCTTC 1 cut(s) 209
HpyCH4V TGCA 3 cut(s) 22, 98, 272
HpyF10VI GCNNNNNNNGC 2 cut(s) 19, 52
HpyF3I CTNAG 1 cut(s) 42
HspAI GCGC 1 cut(s) 154
Kzo9I GATC 1 cut(s) 195
LpnPI CCDG 1 cut(s) 101
LweI GCATC 1 cut(s) 120
MalI GATC 1 cut(s) 197
MboI GATC 1 cut(s) 195
MboII GAAGA 1 cut(s) 89
MhlI GDGCHC 1 cut(s) 274
MlsI TGGCCA 1 cut(s) 164
MluCI AATT 1 cut(s) 49
MluNI TGGCCA 1 cut(s) 164
MlyI GAGTC 1 cut(s) 213
MnlI CCTC 3 cut(s) 136, 177, 252
Mox20I TGGCCA 1 cut(s) 164
MscI TGGCCA 1 cut(s) 164
MseI TTAA 1 cut(s) 189
Msp20I TGGCCA 1 cut(s) 164
MwoI GCNNNNNNNGC 2 cut(s) 19, 52
NdeII GATC 1 cut(s) 195
PfeI GAWTC 1 cut(s) 148
PkrI GCNGC 1 cut(s) 15
PleI GAGTC 1 cut(s) 213
PpsI GAGTC 1 cut(s) 213
PspPI GGNCC 1 cut(s) 85
SaqAI TTAA 1 cut(s) 189
SatI GCNGC 1 cut(s) 14
Sau3AI GATC 1 cut(s) 195
Sau96I GGNCC 1 cut(s) 85
SchI GAGTC 1 cut(s) 213
SduI GDGCHC 1 cut(s) 274
SetI ASST 2 cut(s) 90, 263
SfaNI GCATC 1 cut(s) 120
SgeI CNNG 9 cut(s) 22, 73, 94, 100, 104, 133, 146, 157, 243
SinI GGWCC 1 cut(s) 85
Sse9I AATT 1 cut(s) 49
SsiI CCGC 2 cut(s) 13, 74
TaqI TCGA 1 cut(s) 141
TasI AATT 1 cut(s) 49
TauI GCSGC 1 cut(s) 16
TfiI GAWTC 1 cut(s) 148
Tru1I TTAA 1 cut(s) 189
Tru9I TTAA 1 cut(s) 189
TspDTI ATGAA 1 cut(s) 268
VneI GTGCAC 1 cut(s) 270
VpaK11BI GGWCC 1 cut(s) 85
XcmI CCANNNNNNNNNTGG 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.