pycom12g11300
MYB Family

TSL-kinase interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0005307
Physical Location & Seq
Forward (+)
13517811 .. 13521596
3786 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1587 bp
ATGCAGATAGGGTCAACAGTCATCATGGACTCTGAGGTACATCCTCATCAGGGGAGCTTCTTGGTTCAGGATGGAGGTCCAGTTGTAGCCCCAACCATCTCAACTCATGTCGCACAGCAGCAGCCCATGAAAAAAACAACACGGCAATGGGCTGCTTGGACACATCAAGAGGAGGAAAGTTTCTTTTCTGCGTTACGACAAGTTGGGAAGAATTTTGAAAAGATCACTCGACGTGTTCAAAGTAAAAACAAGGATCAGGTCAGGCATTATTACTATCGTCTTGTGAGGCGTATGAACAAGCTGCTGGGTCCAGAATTGTATTTGGATTCCAAAAATTCTAAAGATACGAATGCTGCTATGCTTCGATGGTGGTCTTTGCTGGAAAAATATAGTTGCAAAGCATCAAAGCTTCACTTGAAGCCCCGAAGGTTTAAGATCTTTATAGAAGCACTGGAACACCAACTCCTGAAAGACCGGAAGAAGAGTGTTAGGAAACGGCCTTCTCAGGGGGAAAACTGCCCTCCTAATGCTCCGAGTACTGTTTCCAATCAGAATAGACCATCAGGACATGATAATCGCACAGTTAAACTGGTTCTTGTTGACAGTCACAATATTCAGAAATTAGGACCTGGAAAAGCATCAATGAAGCGCAATGTAAATATAGGTGTTAATCGTAGCAACAAAGGAGACTCAAGTACCATGAAACCTGCTAGACAACGACGGAAACCAGGTGCTGTCTCGTCAGTGGCGTATAAAAAGTGGGAAAAGGCTGCAATTGCTGGGGTTTCTTTGGTTGCTGATGCTGCTGAGCACCTGGAGCGAGCAGCAACTGATAAAGAAGTTCAGCAGCAGGACAGAAGCACTGAAGGGCAAAGCTGCTCTGTTCCTGTTGGAACCGTCCTGTCCCCTTTGCCCACTTTCCCACAAAAAATTTACGCAGAGAACAATTGTATGAAACTTAAACTCCAGTTGTTCCCAATTGATGATGTTACTAGGAGAGCCTTGGAAATTGATAAACATAATCCACACTTGGAGCTCACTCTTAGTACTCGGAAAAAGATATCATCAGTTTTGGAACACCTAAATCGCAAATGGGGGAATTCAAATGTAGCATCGGGAGAGCTAATGCTTTTCCCTTACAGTGTTCAGAGAGAGAACCTGATGGGTTATCCGAGATGGGCACAAGACTCAGTTGTCAGTGCGGCGGATGTATACTCAACAATTGGAAGCCCTCCAGTTTTTCGTTTAAGGTACGGTTGGTTCTCCAATACTGAGCTCAGGTCTGTTGAATTCCAAGCACCTATAGCATCTTGCAGCATTCAAGGGGAGCACATGAATGTGGAGAAGAGGAACGAGCAGATTGTGGATTCAACTCCTATATCTCATCTATCAACTAACAATCAGTCTGTGGATAAGAACTCTCTTGCTTTGACATCAACTGATGAGCCCAATGAGATAAGTGGCTATGTCTCCTCAAGTCCAAACAATGTGGGGGCCCCTACAGAAATAAGGACATTGCATAGAAAGGAGGCTGGTGATCAGAATACCTCAGTACATCGGGGATCGATCTGGATGGCACGCGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003682 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

529

Amino Acids

59.49

Weight (kDa)

9.76

Isoelectric Point (pI)

49.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 52 - 94 4.7e-08 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1193
Acc36I ACCTGC 1 cut(s) 715
AccI GTMKAC 1 cut(s) 1212
AccII CGCG 1 cut(s) 1581
AciI CCGC 2 cut(s) 1202, 1205
AclWI GGATC 2 cut(s) 261, 1570
AcsI RAATTY 5 cut(s) 211, 334, 930, 1099, 1289
AcuI CTGAAG 1 cut(s) 885
AfaI GTAC 6 cut(s) 39, 538, 697, 1048, 1253, 1554
AfiI CCNNNNNNNGG 2 cut(s) 50, 506
AflIII ACRYGT 1 cut(s) 232
AgsI TTSAA 7 cut(s) 218, 239, 418, 1104, 1289, 1322, 1371
AjiI CACGTC 1 cut(s) 233
AjnI CCWGG 3 cut(s) 628, 727, 813
AloI GAACNNNNNNTCC 2 cut(s) 447, 479
AluBI AGCT 7 cut(s) 57, 301, 409, 876, 1036, 1123, 1276
AluI AGCT 7 cut(s) 57, 301, 409, 876, 1036, 1123, 1276
Alw21I GWGCWC 4 cut(s) 813, 1038, 1278, 1332
Alw26I GTCTC 3 cut(s) 681, 742, 1474
AlwI GGATC 2 cut(s) 261, 1570
AlwNI CAGNNNCTG 2 cut(s) 734, 830
AoxI GGCC 2 cut(s) 497, 1494
ApaI GGGCCC 1 cut(s) 1498
ApoI RAATTY 5 cut(s) 211, 334, 930, 1099, 1289
AspLEI GCGC 1 cut(s) 651
AspS9I GGNCC 5 cut(s) 77, 308, 626, 1494, 1495
AsuHPI GGTGA 1 cut(s) 1547
AvaII GGWCC 3 cut(s) 77, 308, 626
BaeGI GKGCMC 2 cut(s) 1183, 1498
BaeI ACNNNNGTAYC 2 cut(s) 679, 712
BanII GRGCYC 4 cut(s) 1038, 1278, 1449, 1498
Bbv12I GWGCWC 4 cut(s) 813, 1038, 1278, 1332
BccI CCATC 7 cut(s) 65, 104, 360, 568, 1156, 1170, 1567
BceAI ACGGC 2 cut(s) 158, 512
BciT130I CCWGG 3 cut(s) 630, 729, 815
BclI TGATCA 1 cut(s) 1537
BcoDI GTCTC 3 cut(s) 681, 742, 1474
BfaI CTAG 2 cut(s) 711, 993
BfmI CTRYAG 2 cut(s) 1302, 1500
BfuAI ACCTGC 1 cut(s) 715
BglII AGATCT 1 cut(s) 435
BlpI GCTNAGC 1 cut(s) 807
BmcAI AGTACT 2 cut(s) 538, 1048
Bme1390I CCNGG 3 cut(s) 630, 729, 815
Bme18I GGWCC 3 cut(s) 77, 308, 626
BmgBI CACGTC 1 cut(s) 233
BmgT120I GGNCC 5 cut(s) 77, 308, 626, 1494, 1495
BmiI GGNNCC 5 cut(s) 309, 895, 1495, 1496, 1497
BmrFI CCNGG 3 cut(s) 630, 729, 815
BmsI GCATC 5 cut(s) 410, 647, 790, 1121, 1316
BpmI CTGGAG 3 cut(s) 836, 950, 1218
Bpu10I CCTNAGC 1 cut(s) 1277
Bpu1102I GCTNAGC 1 cut(s) 807
BpuEI CTTGAG 2 cut(s) 676, 1459
Bsa29I ATCGAT 1 cut(s) 1565
BsaJI CCNNGG 1 cut(s) 1002
BsaWI WCCGGW 1 cut(s) 474
BsaXI ACNNNNNCTCC 6 cut(s) 66, 96, 447, 477, 948, 978
Bsc4I CCNNNNNNNGG 2 cut(s) 50, 506
Bse1I ACTGG 5 cut(s) 80, 456, 594, 967, 1235
Bse3DI GCAATG 3 cut(s) 152, 658, 1514
BseBI CCWGG 3 cut(s) 630, 729, 815
BseCI ATCGAT 1 cut(s) 1565
BseDI CCNNGG 1 cut(s) 1002
BseGI GGATG 4 cut(s) 40, 76, 1213, 1578
BseLI CCNNNNNNNGG 2 cut(s) 50, 506
BseMI GCAATG 3 cut(s) 152, 658, 1514
BseMII CTCAG 7 cut(s) 24, 518, 798, 1203, 1263, 1291, 1563
BseNI ACTGG 5 cut(s) 80, 456, 594, 967, 1235
BseRI GAGGAG 2 cut(s) 185, 1462
BseSI GKGCMC 2 cut(s) 1183, 1498
BseYI CCCAGC 2 cut(s) 304, 779
Bsh1236I CGCG 1 cut(s) 1581
BshFI GGCC 2 cut(s) 499, 1496
BshVI ATCGAT 1 cut(s) 1565
BsiHKAI GWGCWC 4 cut(s) 813, 1038, 1278, 1332
BsiSI CCGG 1 cut(s) 475
BslFI GGGAC 1 cut(s) 889
BslI CCNNNNNNNGG 2 cut(s) 50, 506
BsmAI GTCTC 3 cut(s) 681, 742, 1474
BsmFI GGGAC 1 cut(s) 889
BsmI GAATGC 2 cut(s) 355, 1317
BsnI GGCC 2 cut(s) 499, 1496
Bsp120I GGGCCC 1 cut(s) 1494
Bsp1286I GDGCHC 7 cut(s) 813, 1038, 1183, 1278, 1332, 1449, 1498
Bsp143I GATC 6 cut(s) 222, 253, 435, 1537, 1562, 1566
Bsp1720I GCTNAGC 1 cut(s) 807
BspACI CCGC 2 cut(s) 1202, 1205
BspANI GGCC 2 cut(s) 499, 1496
BspCNI CTCAG 7 cut(s) 25, 517, 799, 1202, 1264, 1290, 1562
BspDI ATCGAT 1 cut(s) 1565
BspFNI CGCG 1 cut(s) 1581
BspLI GGNNCC 5 cut(s) 309, 895, 1495, 1496, 1497
BspMI ACCTGC 1 cut(s) 715
BspPI GGATC 2 cut(s) 261, 1570
BsrDI GCAATG 3 cut(s) 152, 658, 1514
BsrI ACTGG 5 cut(s) 80, 456, 594, 967, 1235
BssECI CCNNGG 1 cut(s) 1002
BssMI GATC 6 cut(s) 222, 253, 435, 1537, 1562, 1566
BssNAI GTATAC 1 cut(s) 1213
BssT1I CCWWGG 1 cut(s) 1002
Bst1107I GTATAC 1 cut(s) 1213
Bst2UI CCWGG 3 cut(s) 630, 729, 815
Bst4CI ACNGT 7 cut(s) 19, 541, 583, 605, 898, 1142, 1256
Bst6I CTCTTC 2 cut(s) 476, 1340
BstC8I GCNNGC 2 cut(s) 822, 1579
BstDEI CTNAG 8 cut(s) 33, 504, 807, 1043, 1189, 1272, 1277, 1549
BstF5I GGATG 4 cut(s) 40, 76, 1213, 1578
BstFNI CGCG 1 cut(s) 1581
BstHHI GCGC 1 cut(s) 651
BstKTI GATC 6 cut(s) 225, 256, 438, 1540, 1565, 1569
BstMAI GTCTC 3 cut(s) 681, 742, 1474
BstMBI GATC 6 cut(s) 222, 253, 435, 1537, 1562, 1566
BstMWI GCNNNNNNNGC 4 cut(s) 776, 803, 817, 1304
BstNI CCWGG 3 cut(s) 630, 729, 815
BstSCI CCNGG 3 cut(s) 628, 727, 813
BstSFI CTRYAG 2 cut(s) 1302, 1500
BstSLI GKGCMC 2 cut(s) 1183, 1498
BstUI CGCG 1 cut(s) 1581
BstX2I RGATCY 1 cut(s) 435
BstYI RGATCY 1 cut(s) 435
BstZ17I GTATAC 1 cut(s) 1213
Bsu15I ATCGAT 1 cut(s) 1565
BsuRI GGCC 2 cut(s) 499, 1496
BsuTUI ATCGAT 1 cut(s) 1565
BtrI CACGTC 1 cut(s) 233
BtsCI GGATG 4 cut(s) 40, 76, 1213, 1578
BtsIMutI CAGTG 5 cut(s) 449, 750, 861, 1147, 1204
BveI ACCTGC 1 cut(s) 715
Cac8I GCNNGC 2 cut(s) 822, 1579
CaiI CAGNNNCTG 2 cut(s) 734, 830
CfoI GCGC 1 cut(s) 651
Cfr13I GGNCC 5 cut(s) 77, 308, 626, 1494, 1495
ClaI ATCGAT 1 cut(s) 1565
CsiI ACCWGGT 1 cut(s) 727
Csp6I GTAC 6 cut(s) 38, 537, 696, 1047, 1252, 1553
CspCI CAANNNNNGTGG 2 cut(s) 1470, 1505
CviAII CATG 6 cut(s) 25, 107, 127, 569, 700, 1333
CviQI GTAC 6 cut(s) 38, 537, 696, 1047, 1252, 1553
DdeI CTNAG 8 cut(s) 33, 504, 807, 1043, 1189, 1272, 1277, 1549
DpnI GATC 6 cut(s) 224, 255, 437, 1539, 1564, 1568
DpnII GATC 6 cut(s) 222, 253, 435, 1537, 1562, 1566
DrdI GACNNNNNNGTC 1 cut(s) 1193
DseDI GACNNNNNNGTC 1 cut(s) 1193
Eam1104I CTCTTC 2 cut(s) 476, 1340
EarI CTCTTC 2 cut(s) 476, 1340
EciI GGCGGA 1 cut(s) 1220
Ecl136II GAGCTC 2 cut(s) 1036, 1276
Eco130I CCWWGG 1 cut(s) 1002
Eco24I GRGCYC 4 cut(s) 1038, 1278, 1449, 1498
Eco32I GATATC 1 cut(s) 1062
Eco47I GGWCC 3 cut(s) 77, 308, 626
Eco53kI GAGCTC 2 cut(s) 1036, 1276
Eco57I CTGAAG 1 cut(s) 885
EcoICRI GAGCTC 2 cut(s) 1036, 1276
EcoO109I RGGNCCY 3 cut(s) 626, 1494, 1495
EcoRI GAATTC 2 cut(s) 1099, 1289
EcoRII CCWGG 3 cut(s) 628, 727, 813
EcoRV GATATC 1 cut(s) 1062
EcoT14I CCWWGG 1 cut(s) 1002
EcoT38I GRGCYC 4 cut(s) 1038, 1278, 1449, 1498
ErhI CCWWGG 1 cut(s) 1002
FaeI CATG 6 cut(s) 28, 110, 130, 572, 703, 1336
FalI AAGNNNNNCTT 2 cut(s) 398, 430
FaqI GGGAC 1 cut(s) 889
FatI CATG 6 cut(s) 24, 106, 126, 568, 699, 1332
FbaI TGATCA 1 cut(s) 1537
FblI GTMKAC 1 cut(s) 1212
FokI GGATG 3 cut(s) 27, 83, 1220
FriOI GRGCYC 4 cut(s) 1038, 1278, 1449, 1498
FspBI CTAG 2 cut(s) 711, 993
GlaI GCGC 1 cut(s) 650
GsaI CCCAGC 2 cut(s) 308, 783
GsuI CTGGAG 3 cut(s) 836, 950, 1218
HaeIII GGCC 2 cut(s) 499, 1496
HapII CCGG 1 cut(s) 475
HhaI GCGC 1 cut(s) 651
Hin1II CATG 6 cut(s) 28, 110, 130, 572, 703, 1336
Hin6I GCGC 1 cut(s) 649
HinP1I GCGC 1 cut(s) 649
HincII GTYRAC 2 cut(s) 15, 601
HindII GTYRAC 2 cut(s) 15, 601
HindIII AAGCTT 1 cut(s) 407
HinfI GANTC 5 cut(s) 29, 326, 689, 1187, 1367
HpaII CCGG 1 cut(s) 475
HphI GGTGA 1 cut(s) 1547
Hpy166II GTNNAC 3 cut(s) 15, 601, 1213
Hpy188I TCNGA 8 cut(s) 34, 534, 552, 618, 1053, 1149, 1173, 1542
Hpy188III TCNNGA 7 cut(s) 68, 167, 311, 466, 564, 1116, 1570
Hpy8I GTNNAC 3 cut(s) 15, 601, 1213
Hpy99I CGWCG 2 cut(s) 234, 723
HpyAV CCTTC 3 cut(s) 420, 510, 860
HpyCH4III ACNGT 7 cut(s) 19, 541, 583, 605, 898, 1142, 1256
HpyCH4IV ACGT 1 cut(s) 232
HpyCH4V TGCA 5 cut(s) 4, 396, 773, 1314, 1519
HpyF10VI GCNNNNNNNGC 4 cut(s) 776, 803, 817, 1304
HpyF3I CTNAG 8 cut(s) 33, 504, 807, 1043, 1189, 1272, 1277, 1549
HpySE526I ACGT 1 cut(s) 232
Hsp92II CATG 6 cut(s) 28, 110, 130, 572, 703, 1336
HspAI GCGC 1 cut(s) 649
Ksp22I TGATCA 1 cut(s) 1537
Kzo9I GATC 6 cut(s) 222, 253, 435, 1537, 1562, 1566
LmnI GCTCC 5 cut(s) 54, 535, 817, 1033, 1327
LweI GCATC 5 cut(s) 410, 647, 790, 1121, 1316
MabI ACCWGGT 1 cut(s) 727
MaeI CTAG 2 cut(s) 711, 993
MaeII ACGT 1 cut(s) 232
MaeIII GTNAC 3 cut(s) 192, 605, 988
MalI GATC 6 cut(s) 224, 255, 437, 1539, 1564, 1568
MboI GATC 6 cut(s) 222, 253, 435, 1537, 1562, 1566
MboII GAAGA 4 cut(s) 220, 490, 493, 1357
MfeI CAATTG 4 cut(s) 774, 946, 978, 1221
MflI RGATCY 1 cut(s) 435
MhlI GDGCHC 7 cut(s) 813, 1038, 1183, 1278, 1332, 1449, 1498
MlyI GAGTC 3 cut(s) 23, 683, 1181
MmeI TCCRAC 1 cut(s) 871
MseI TTAA 6 cut(s) 432, 585, 669, 960, 1247, 1585
MslI CAYNNNNRTG 3 cut(s) 145, 1335, 1337
MspI CCGG 1 cut(s) 475
MspR9I CCNGG 3 cut(s) 630, 729, 815
MunI CAATTG 4 cut(s) 774, 946, 978, 1221
Mva1269I GAATGC 2 cut(s) 355, 1317
MvaI CCWGG 3 cut(s) 630, 729, 815
MvnI CGCG 1 cut(s) 1581
MwoI GCNNNNNNNGC 4 cut(s) 776, 803, 817, 1304
NdeII GATC 6 cut(s) 222, 253, 435, 1537, 1562, 1566
NlaIII CATG 6 cut(s) 28, 110, 130, 572, 703, 1336
NlaIV GGNNCC 5 cut(s) 309, 895, 1495, 1496, 1497
NmuCI GTSAC 1 cut(s) 605
PcsI WCGNNNNNNNCGW 1 cut(s) 746
PctI GAATGC 2 cut(s) 355, 1317
PfeI GAWTC 2 cut(s) 326, 1367
PleI GAGTC 3 cut(s) 23, 683, 1181
PpsI GAGTC 3 cut(s) 23, 683, 1181
PpuMI RGGWCCY 1 cut(s) 626
Psp124BI GAGCTC 2 cut(s) 1038, 1278
Psp5II RGGWCCY 1 cut(s) 626
Psp6I CCWGG 3 cut(s) 628, 727, 813
PspFI CCCAGC 2 cut(s) 304, 779
PspGI CCWGG 3 cut(s) 628, 727, 813
PspN4I GGNNCC 5 cut(s) 309, 895, 1495, 1496, 1497
PspOMI GGGCCC 1 cut(s) 1494
PspPI GGNCC 5 cut(s) 77, 308, 626, 1494, 1495
PspPPI RGGWCCY 1 cut(s) 626
PsrI GAACNNNNNNTAC 2 cut(s) 1244, 1276
PstNI CAGNNNCTG 2 cut(s) 734, 830
PsuI RGATCY 1 cut(s) 435
RsaI GTAC 6 cut(s) 39, 538, 697, 1048, 1253, 1554
RsaNI GTAC 6 cut(s) 38, 537, 696, 1047, 1252, 1553
RseI CAYNNNNRTG 3 cut(s) 145, 1335, 1337
SacI GAGCTC 2 cut(s) 1038, 1278
SaqAI TTAA 6 cut(s) 432, 585, 669, 960, 1247, 1585
Sau3AI GATC 6 cut(s) 222, 253, 435, 1537, 1562, 1566
Sau96I GGNCC 5 cut(s) 77, 308, 626, 1494, 1495
ScaI AGTACT 2 cut(s) 538, 1048
SchI GAGTC 3 cut(s) 23, 683, 1181
ScrFI CCNGG 3 cut(s) 630, 729, 815
SduI GDGCHC 7 cut(s) 813, 1038, 1183, 1278, 1332, 1449, 1498
SexAI ACCWGGT 1 cut(s) 727
SfaNI GCATC 5 cut(s) 410, 647, 790, 1121, 1316
SfcI CTRYAG 2 cut(s) 1302, 1500
SinI GGWCC 3 cut(s) 77, 308, 626
SmiMI CAYNNNNRTG 3 cut(s) 145, 1335, 1337
SmlI CTYRAG 2 cut(s) 691, 1474
SmoI CTYRAG 2 cut(s) 691, 1474
SsiI CCGC 2 cut(s) 1202, 1205
SspI AATATT 1 cut(s) 613
SspMI CTAG 2 cut(s) 711, 993
SstI GAGCTC 2 cut(s) 1038, 1278
StyD4I CCNGG 3 cut(s) 628, 727, 813
StyI CCWWGG 1 cut(s) 1002
TaaI ACNGT 7 cut(s) 19, 541, 583, 605, 898, 1142, 1256
TaiI ACGT 1 cut(s) 235
TaqI TCGA 3 cut(s) 229, 364, 1565
TatI WGTACW 3 cut(s) 536, 1046, 1552
TauI GCSGC 1 cut(s) 1205
TfiI GAWTC 2 cut(s) 326, 1367
Tru1I TTAA 6 cut(s) 432, 585, 669, 960, 1247, 1585
Tru9I TTAA 6 cut(s) 432, 585, 669, 960, 1247, 1585
TscAI CASTG 5 cut(s) 456, 750, 868, 1147, 1204
TseFI GTSAC 1 cut(s) 605
Tsp45I GTSAC 1 cut(s) 605
TspDTI ATGAA 6 cut(s) 143, 308, 659, 716, 968, 1349
TspGWI ACGGA 1 cut(s) 736
TspRI CASTG 5 cut(s) 456, 750, 868, 1147, 1204
VpaK11BI GGWCC 3 cut(s) 77, 308, 626
XapI RAATTY 5 cut(s) 211, 334, 930, 1099, 1289
XmiI GTMKAC 1 cut(s) 1212
XspI CTAG 2 cut(s) 711, 993
ZrmI AGTACT 2 cut(s) 538, 1048
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.