pycom12g11310
MYB Family

TSL-kinase interacting protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0005307
Physical Location & Seq
Forward (+)
13521806 .. 13522682
877 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g11310.3

Sequence Viewer

Length: 543 bp
ATGGGATGTCAACATATTATGGAATCCCATGGATCTTCTATCTGGGATGCTGAAGAAACATGTGATGGCTTCTCATTTCCAAAGAATCCTGTTTTCTGTCAAGAAGTTCCTAGTTTACCTGGGGTTGCTCCCCCAGGGCCATGCAAACAGACAGTTTGTACAAGCTCAGCGGGATCTGATCTTATGGTTGAGGACATACCTGTCGTTGACAGGCCCATTGATCCTCCCCAAGGAGACTGTGTGGACCAGTGTCAGCCGGATCTGCATATTGAGAGTTCAGTAAAAGATTTTAACGCATTAGCAGATATATATTGGCCTGAATCTTTAGGACCATTAGATTTGGAGATGCCGTCCTCCAAATACAATAACGAAGAATTCATGCTGAGTGATAGCCTTAGTGGATTGAACCGCCTTATTGCCAGCAGTCTTGATGCATTTCAAAATTGCTCCTTTTTCGGGGTGAATAAGAAAGAAGCAACACCATCAGCCGAAGGTCGAGAAACTGCATCCTTTTCAGATTTCAAAATTGGCAACGGAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003682 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

181

Amino Acids

19.44

Weight (kDa)

4.18

Isoelectric Point (pI)

45.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 200
AciI CCGC 2 cut(s) 170, 409
AclWI GGATC 4 cut(s) 40, 181, 215, 267
AcsI RAATTY 1 cut(s) 374
AcuI CTGAAG 1 cut(s) 72
AfaI GTAC 1 cut(s) 160
AfiI CCNNNNNNNGG 2 cut(s) 230, 456
AflIII ACRYGT 1 cut(s) 59
AgsI TTSAA 3 cut(s) 406, 440, 523
AjnI CCWGG 2 cut(s) 118, 133
AluBI AGCT 1 cut(s) 165
AluI AGCT 1 cut(s) 165
Alw26I GTCTC 1 cut(s) 228
AlwI GGATC 4 cut(s) 40, 181, 215, 267
AoxI GGCC 3 cut(s) 137, 212, 314
ApoI RAATTY 1 cut(s) 374
AspS9I GGNCC 4 cut(s) 137, 213, 244, 329
AsuHPI GGTGA 1 cut(s) 472
AvaII GGWCC 2 cut(s) 244, 329
BccI CCATC 2 cut(s) 59, 490
BceAI ACGGC 1 cut(s) 334
BciT130I CCWGG 2 cut(s) 120, 135
BcoDI GTCTC 1 cut(s) 228
BfaI CTAG 1 cut(s) 111
BlpI GCTNAGC 1 cut(s) 166
Bme1390I CCNGG 2 cut(s) 120, 135
Bme18I GGWCC 2 cut(s) 244, 329
BmgT120I GGNCC 4 cut(s) 137, 213, 244, 329
BmrFI CCNGG 2 cut(s) 120, 135
BmsI GCATC 4 cut(s) 37, 336, 421, 515
BoxI GACNNNNGTC 1 cut(s) 249
Bpu1102I GCTNAGC 1 cut(s) 166
BsaJI CCNNGG 5 cut(s) 28, 119, 133, 134, 229
BsaXI ACNNNNNCTCC 4 cut(s) 225, 255, 335, 365
Bsc4I CCNNNNNNNGG 2 cut(s) 230, 456
Bse1I ACTGG 1 cut(s) 247
BseBI CCWGG 2 cut(s) 120, 135
BseDI CCNNGG 5 cut(s) 28, 119, 133, 134, 229
BseGI GGATG 3 cut(s) 11, 52, 506
BseLI CCNNNNNNNGG 2 cut(s) 230, 456
BseMII CTCAG 2 cut(s) 180, 374
BseNI ACTGG 1 cut(s) 247
BshFI GGCC 3 cut(s) 139, 214, 316
BsiSI CCGG 1 cut(s) 257
BslI CCNNNNNNNGG 2 cut(s) 230, 456
BsmAI GTCTC 1 cut(s) 228
BsnI GGCC 3 cut(s) 139, 214, 316
Bsp1407I TGTACA 1 cut(s) 158
Bsp143I GATC 5 cut(s) 32, 173, 178, 220, 259
Bsp1720I GCTNAGC 1 cut(s) 166
Bsp19I CCATGG 1 cut(s) 28
BspACI CCGC 2 cut(s) 170, 409
BspANI GGCC 3 cut(s) 139, 214, 316
BspCNI CTCAG 2 cut(s) 179, 375
BspPI GGATC 4 cut(s) 40, 181, 215, 267
BsrGI TGTACA 1 cut(s) 158
BsrI ACTGG 1 cut(s) 247
BssECI CCNNGG 5 cut(s) 28, 119, 133, 134, 229
BssMI GATC 5 cut(s) 32, 173, 178, 220, 259
BssT1I CCWWGG 2 cut(s) 28, 229
Bst2UI CCWGG 2 cut(s) 120, 135
Bst4CI ACNGT 2 cut(s) 154, 239
BstAUI TGTACA 1 cut(s) 158
BstC8I GCNNGC 1 cut(s) 421
BstDEI CTNAG 3 cut(s) 166, 383, 395
BstDSI CCRYGG 1 cut(s) 28
BstENI CCTNNNNNAGG 1 cut(s) 228
BstF5I GGATG 3 cut(s) 11, 52, 506
BstKTI GATC 5 cut(s) 35, 176, 181, 223, 262
BstMAI GTCTC 1 cut(s) 228
BstMBI GATC 5 cut(s) 32, 173, 178, 220, 259
BstMWI GCNNNNNNNGC 1 cut(s) 262
BstNI CCWGG 2 cut(s) 120, 135
BstNSI RCATGY 1 cut(s) 63
BstPAI GACNNNNGTC 1 cut(s) 249
BstSCI CCNGG 2 cut(s) 118, 133
BstX2I RGATCY 3 cut(s) 32, 173, 259
BstYI RGATCY 3 cut(s) 32, 173, 259
BsuRI GGCC 3 cut(s) 139, 214, 316
BtgI CCRYGG 1 cut(s) 28
BtsCI GGATG 3 cut(s) 11, 52, 506
BtsIMutI CAGTG 1 cut(s) 254
Cac8I GCNNGC 1 cut(s) 421
Cfr13I GGNCC 4 cut(s) 137, 213, 244, 329
Csp6I GTAC 1 cut(s) 159
CviAII CATG 4 cut(s) 29, 60, 141, 379
CviJI RGCY 8 cut(s) 69, 139, 165, 214, 256, 316, 393, 488
CviKI_1 RGCY 8 cut(s) 69, 139, 165, 214, 256, 316, 393, 488
CviQI GTAC 1 cut(s) 159
DdeI CTNAG 3 cut(s) 166, 383, 395
DpnI GATC 5 cut(s) 34, 175, 180, 222, 261
DpnII GATC 5 cut(s) 32, 173, 178, 220, 259
DrdI GACNNNNNNGTC 1 cut(s) 200
DseDI GACNNNNNNGTC 1 cut(s) 200
Eco130I CCWWGG 2 cut(s) 28, 229
Eco47I GGWCC 2 cut(s) 244, 329
Eco57I CTGAAG 1 cut(s) 72
EcoNI CCTNNNNNAGG 1 cut(s) 228
EcoRI GAATTC 1 cut(s) 374
EcoRII CCWGG 2 cut(s) 118, 133
EcoT14I CCWWGG 2 cut(s) 28, 229
EcoT22I ATGCAT 1 cut(s) 436
ErhI CCWWGG 2 cut(s) 28, 229
FaeI CATG 4 cut(s) 32, 63, 144, 382
FatI CATG 4 cut(s) 28, 59, 140, 378
FauI CCCGC 1 cut(s) 163
FokI GGATG 3 cut(s) 18, 59, 493
FspBI CTAG 1 cut(s) 111
HaeIII GGCC 3 cut(s) 139, 214, 316
HapII CCGG 1 cut(s) 257
Hin1II CATG 4 cut(s) 32, 63, 144, 382
HincII GTYRAC 2 cut(s) 11, 208
HindII GTYRAC 2 cut(s) 11, 208
HinfI GANTC 3 cut(s) 23, 85, 320
HpaII CCGG 1 cut(s) 257
HphI GGTGA 1 cut(s) 472
Hpy166II GTNNAC 4 cut(s) 11, 116, 208, 244
Hpy188I TCNGA 2 cut(s) 178, 517
Hpy188III TCNNGA 3 cut(s) 101, 428, 497
Hpy8I GTNNAC 4 cut(s) 11, 116, 208, 244
HpyAV CCTTC 1 cut(s) 485
HpyCH4III ACNGT 2 cut(s) 154, 239
HpyCH4V TGCA 4 cut(s) 144, 265, 434, 506
HpyF10VI GCNNNNNNNGC 1 cut(s) 262
HpyF3I CTNAG 3 cut(s) 166, 383, 395
Hsp92II CATG 4 cut(s) 32, 63, 144, 382
Kzo9I GATC 5 cut(s) 32, 173, 178, 220, 259
LmnI GCTCC 2 cut(s) 133, 452
LweI GCATC 4 cut(s) 37, 336, 421, 515
MaeI CTAG 1 cut(s) 111
MalI GATC 5 cut(s) 34, 175, 180, 222, 261
MboI GATC 5 cut(s) 32, 173, 178, 220, 259
MboII GAAGA 3 cut(s) 27, 65, 383
MflI RGATCY 3 cut(s) 32, 173, 259
MluCI AATT 3 cut(s) 374, 442, 525
MnlI CCTC 3 cut(s) 184, 234, 364
Mph1103I ATGCAT 1 cut(s) 436
MseI TTAA 1 cut(s) 291
MspA1I CMGCKG 1 cut(s) 170
MspI CCGG 1 cut(s) 257
MspR9I CCNGG 2 cut(s) 120, 135
MvaI CCWGG 2 cut(s) 120, 135
MwoI GCNNNNNNNGC 1 cut(s) 262
NcoI CCATGG 1 cut(s) 28
NdeII GATC 5 cut(s) 32, 173, 178, 220, 259
NlaIII CATG 4 cut(s) 32, 63, 144, 382
NsiI ATGCAT 1 cut(s) 436
NspI RCATGY 1 cut(s) 63
PasI CCCWGGG 1 cut(s) 134
PciI ACATGT 1 cut(s) 59
PfeI GAWTC 3 cut(s) 23, 85, 320
PscI ACATGT 1 cut(s) 59
PshAI GACNNNNGTC 1 cut(s) 249
Psp6I CCWGG 2 cut(s) 118, 133
PspGI CCWGG 2 cut(s) 118, 133
PspPI GGNCC 4 cut(s) 137, 213, 244, 329
PsuI RGATCY 3 cut(s) 32, 173, 259
RsaI GTAC 1 cut(s) 160
RsaNI GTAC 1 cut(s) 159
SaqAI TTAA 1 cut(s) 291
Sau3AI GATC 5 cut(s) 32, 173, 178, 220, 259
Sau96I GGNCC 4 cut(s) 137, 213, 244, 329
ScrFI CCNGG 2 cut(s) 120, 135
SetI ASST 4 cut(s) 121, 167, 202, 496
SfaNI GCATC 4 cut(s) 37, 336, 421, 515
SinI GGWCC 2 cut(s) 244, 329
Sse9I AATT 3 cut(s) 374, 442, 525
SsiI CCGC 2 cut(s) 170, 409
SspMI CTAG 1 cut(s) 111
StyD4I CCNGG 2 cut(s) 118, 133
StyI CCWWGG 2 cut(s) 28, 229
TaaI ACNGT 2 cut(s) 154, 239
TaqI TCGA 1 cut(s) 496
TasI AATT 3 cut(s) 374, 442, 525
TatI WGTACW 1 cut(s) 158
TfiI GAWTC 3 cut(s) 23, 85, 320
Tru1I TTAA 1 cut(s) 291
Tru9I TTAA 1 cut(s) 291
TscAI CASTG 1 cut(s) 254
TspDTI ATGAA 1 cut(s) 367
TspRI CASTG 1 cut(s) 254
VpaK11BI GGWCC 2 cut(s) 244, 329
XagI CCTNNNNNAGG 1 cut(s) 228
XapI RAATTY 1 cut(s) 374
XceI RCATGY 1 cut(s) 63
XspI CTAG 1 cut(s) 111
Zsp2I ATGCAT 1 cut(s) 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.