pycom12g15830

LIMR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0011383
Physical Location & Seq
Reverse (-)
18272402 .. 18275980
3579 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1461 bp
ATGGGCGATTTCAACCTCGCGCTCGTCATCGTCGCTATTATCCTCTGCATCATCGTCTTCGTCTTCAATGTCTACCTGCTCGTCAATTACCAGCACCCCGACGACAAGAACCAGGCCTACTTCCCCAAATTCGTCGTCGTTTTGGGGCTCTCCGTCGCCGCTATTTCAATACTCATGCTACCGGCCGACGTGGCCAACCGCCAGGCGTGTCGCCACGCGATTTATAATGGCGCGTGCAATCTCACTCTTCCGATGAAGGATCTGTGGCTCGCAATCTATATCCTTGACGCCGTGCTCGTGTTCTTCATCATCCCCTTCGCTATGTTCTACTACGAAGGAGACCAGGACAAGAGTGTTGGAAAACGGATTGGAAGCGCGTTGTTGTGGGTTGCGGTGACCGCCGTTGTCTGCGCTCTCGTGCTCGGAATCTTATACGGGCTAGTTGGGAAGGTGGACTTTACTGTTAGGCACCTCTCTTCTTCCACAACTTCCTTTCCCAGTTCATTTCAATTTTCTAGCAGTAATGAATGCATAAACGGCGCAAAGGCGTGCTCTGCATATCTTGCAAGTGATACGTCAGAGAAAACATGGACAATGCGTACTACCTTTCCAGAATATGTCGTTGCTCTTGCTACCATTGTTGGATCCGTGCTTTTTACTATATTCGGTGGTATTGGCATTGCTTGTCTACCGCTGGGACTCATATTTTCATTCATTCGGCGTCCAAAGGCTGTTATTACTCGGTCACAGTATATCAAGGAAGCTACTGAACTGGGCAAGAAAGCAAAAGAGTTGAAGAAAGCAGCTGATGCGCTTCATCAAGAAGAAAGAGGCGGCTCTAAGGGTAGAAAATGGCGTAAAAATGTAAAGGCAGTAGAGAAGGAGTTGCTTCAATTAGAAGAAGATGTAAAACTTTTAGAAGAGGTGTATCCTCAAGGAGAGAAAGCTGAGACTACTTGGGCTATGACAGTTCTTGGGTACTTGGCAAAACTTGTGTTGGGAATCTTAGGATTGATTGTTTCAGTAGCTTGGATTGCTCATATCGTCATATATCTATTGATTAGCCCTCCTCTTTCTCCTTTCTTGAACGAAGTTTTCATCAAGCTGGATGATGTTTGGGGTCTTTTAGGTACTGTTGCGTTTGCATTCTTCTGCTTCTACCTTCTGCTTGCAGTCATTGCTGGGGCGATGATGCTTGGGCTGAAATTAGTTTTCATCACAATTCATCCGATGAATGTAATTCAGTTCTGCTCCACGGCGTTTGCATACTATGCTCAAGCAACCGCAGCACAAGAAATATTTGGTCACACCTTGGAATCTCTTCGTGGAATTAGATATCTGTACAAGTACAATGTGTTCCAAATTGCATTTATTGTCCTGGCAGGACTGACGTTTGTGTACTATGCTGCTTTTGGATGGAGAAGAAAAAAACCTAGCGGCAGGTTCCAACTTTCCTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04977 map04977
Pfam Domains
Protein Families
LMBR1

Protein Analysis

487

Amino Acids

53.92

Weight (kDa)

9.0

Isoelectric Point (pI)

32.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LMBR1 PF04791 9 - 274 3.9e-45 LMBR1-like membrane protein
LMBR1 PF04791 267 - 464 3.1e-20 LMBR1-like membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011383)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G08930 AT5G01460
fragaria_vesca FvH4_6g11450 FvH4_6g11450 FvH4_6g11450
malus_domestica MD04G1152700.v1.1 MD12G1166000.v1.1
prunus_persica Prupe.6G277300_v2.0.a1
pyrus_communis pycom04g13840 pycom12g15830
rosa_chinensis RchiOBHm_Chr3g0463141
rosa_laevigata RLG00000024810
rosa_multiflora Rmu_sc0008124.1_g000007
rosa_roxburghii Rroxscaffold_6G00416940
rosa_rugosa Rorug03G0061700
rosa_samantha Rh3AG119900 Rh3BG124300 Rh3CG126300 Rh3DG125300
rosa_wichuraiana Rw3G010140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 225
Acc36I ACCTGC 2 cut(s) 84, 1431
AccB1I GGYRCC 1 cut(s) 468
AccI GTMKAC 2 cut(s) 72, 688
AccII CGCG 4 cut(s) 20, 218, 233, 377
AciI CCGC 8 cut(s) 159, 199, 392, 399, 692, 834, 1284, 1437
AclWI GGATC 3 cut(s) 267, 639, 652
AcoI YGGCCR 2 cut(s) 183, 192
AcsI RAATTY 1 cut(s) 128
AcyI GRCGYC 2 cut(s) 288, 721
AfaI GTAC 6 cut(s) 601, 980, 1132, 1343, 1349, 1400
AgsI TTSAA 7 cut(s) 13, 67, 168, 509, 796, 893, 1087
AjiI CACGTC 1 cut(s) 190
AjnI CCWGG 4 cut(s) 111, 201, 342, 1377
AluBI AGCT 5 cut(s) 764, 806, 947, 1028, 1105
AluI AGCT 5 cut(s) 764, 806, 947, 1028, 1105
Alw21I GWGCWC 3 cut(s) 297, 423, 554
Alw26I GTCTC 2 cut(s) 333, 944
AlwI GGATC 3 cut(s) 267, 639, 652
AoxI GGCC 3 cut(s) 114, 183, 192
ApeKI GCWGC 3 cut(s) 803, 1286, 1406
ApoI RAATTY 1 cut(s) 128
ArsI GACNNNNNNTTYG 2 cut(s) 120, 152
Asp700I GAANNNNTTC 1 cut(s) 1320
AspLEI GCGC 6 cut(s) 22, 233, 377, 413, 542, 814
AsuHPI GGTGA 1 cut(s) 406
BalI TGGCCA 1 cut(s) 194
BamHI GGATCC 1 cut(s) 644
BanI GGYRCC 1 cut(s) 468
BanII GRGCYC 1 cut(s) 150
BauI CACGAG 2 cut(s) 296, 416
BbsI GAAGAC 2 cut(s) 49, 55
Bbv12I GWGCWC 3 cut(s) 297, 423, 554
BbvI GCAGC 3 cut(s) 815, 1298, 1393
BccI CCATC 1 cut(s) 1410
BceAI ACGGC 4 cut(s) 275, 386, 553, 1272
BciT130I CCWGG 4 cut(s) 113, 203, 344, 1379
BciVI GTATCC 1 cut(s) 939
BcoDI GTCTC 2 cut(s) 333, 944
BfaI CTAG 3 cut(s) 440, 516, 1434
BfuAI ACCTGC 2 cut(s) 84, 1431
BfuI GTATCC 1 cut(s) 939
BglI GCCNNNNNGGC 1 cut(s) 191
BisI GCNGC 6 cut(s) 159, 804, 835, 1287, 1407, 1438
BlsI GCNGC 6 cut(s) 160, 805, 836, 1288, 1408, 1439
Bme1390I CCNGG 4 cut(s) 113, 203, 344, 1379
BmgBI CACGTC 1 cut(s) 190
BmiI GGNNCC 3 cut(s) 470, 646, 1445
BmrFI CCNGG 4 cut(s) 113, 203, 344, 1379
BmrI ACTGGG 2 cut(s) 492, 782
BmsI GCATC 3 cut(s) 57, 799, 1182
BmuI ACTGGG 2 cut(s) 492, 782
BpiI GAAGAC 2 cut(s) 49, 55
BpuEI CTTGAG 2 cut(s) 918, 1260
BsaHI GRCGYC 2 cut(s) 288, 721
BsaI GGTCTC 1 cut(s) 333
BsaJI CCNNGG 2 cut(s) 1254, 1311
Bse118I RCCGGY 1 cut(s) 181
Bse1I ACTGG 2 cut(s) 498, 777
Bse3DI GCAATG 2 cut(s) 678, 1176
BseBI CCWGG 4 cut(s) 113, 203, 344, 1379
BseDI CCNNGG 2 cut(s) 1254, 1311
BseGI GGATG 4 cut(s) 309, 1114, 1225, 1421
BseMI GCAATG 2 cut(s) 678, 1176
BseMII CTCAG 1 cut(s) 939
BseNI ACTGG 2 cut(s) 498, 777
BseRI GAGGAG 1 cut(s) 1059
BseX3I CGGCCG 1 cut(s) 183
BseXI GCAGC 3 cut(s) 815, 1298, 1393
BseYI CCCAGC 2 cut(s) 694, 1181
Bsh1236I CGCG 4 cut(s) 20, 218, 233, 377
Bsh1285I CGRYCG 1 cut(s) 186
BshFI GGCC 3 cut(s) 116, 185, 194
BshNI GGYRCC 1 cut(s) 468
BsiEI CGRYCG 1 cut(s) 186
BsiHKAI GWGCWC 3 cut(s) 297, 423, 554
BsiSI CCGG 1 cut(s) 182
BslFI GGGAC 1 cut(s) 711
BsmAI GTCTC 2 cut(s) 333, 944
BsmFI GGGAC 1 cut(s) 711
BsmI GAATGC 2 cut(s) 533, 1145
BsnI GGCC 3 cut(s) 116, 185, 194
Bso31I GGTCTC 1 cut(s) 333
Bsp1286I GDGCHC 4 cut(s) 150, 297, 423, 554
Bsp1407I TGTACA 1 cut(s) 1341
Bsp143I GATC 2 cut(s) 259, 644
BspACI CCGC 8 cut(s) 159, 199, 392, 399, 692, 834, 1284, 1437
BspANI GGCC 3 cut(s) 116, 185, 194
BspCNI CTCAG 1 cut(s) 940
BspFNI CGCG 4 cut(s) 20, 218, 233, 377
BspLI GGNNCC 3 cut(s) 470, 646, 1445
BspMI ACCTGC 2 cut(s) 84, 1431
BspPI GGATC 3 cut(s) 267, 639, 652
BspT107I GGYRCC 1 cut(s) 468
BspTNI GGTCTC 1 cut(s) 333
BsrDI GCAATG 2 cut(s) 678, 1176
BsrFI RCCGGY 1 cut(s) 181
BsrGI TGTACA 1 cut(s) 1341
BsrI ACTGG 2 cut(s) 498, 777
BssAI RCCGGY 1 cut(s) 181
BssECI CCNNGG 2 cut(s) 1254, 1311
BssMI GATC 2 cut(s) 259, 644
BssNI GRCGYC 2 cut(s) 288, 721
BssSI CACGAG 2 cut(s) 296, 416
BssT1I CCWWGG 1 cut(s) 1311
Bst2BI CACGAG 2 cut(s) 296, 416
Bst2UI CCWGG 4 cut(s) 113, 203, 344, 1379
Bst4CI ACNGT 4 cut(s) 463, 750, 970, 1135
Bst6I CTCTTC 4 cut(s) 252, 481, 915, 1326
BstACI GRCGYC 2 cut(s) 288, 721
BstAPI GCANNNNNTGC 4 cut(s) 563, 809, 1178, 1271
BstAUI TGTACA 1 cut(s) 1341
BstC8I GCNNGC 4 cut(s) 235, 270, 550, 1170
BstDEI CTNAG 3 cut(s) 840, 948, 1006
BstDSI CCRYGG 1 cut(s) 1254
BstEII GGTNACC 1 cut(s) 394
BstF5I GGATG 4 cut(s) 309, 1114, 1225, 1421
BstFNI CGCG 4 cut(s) 20, 218, 233, 377
BstHHI GCGC 6 cut(s) 22, 233, 377, 413, 542, 814
BstKTI GATC 2 cut(s) 262, 647
BstMAI GTCTC 2 cut(s) 333, 944
BstMBI GATC 2 cut(s) 259, 644
BstMCI CGRYCG 1 cut(s) 186
BstNI CCWGG 4 cut(s) 113, 203, 344, 1379
BstPI GGTNACC 1 cut(s) 394
BstSCI CCNGG 4 cut(s) 111, 201, 342, 1377
BstUI CGCG 4 cut(s) 20, 218, 233, 377
BstV1I GCAGC 3 cut(s) 815, 1298, 1393
BstV2I GAAGAC 2 cut(s) 49, 55
BstX2I RGATCY 2 cut(s) 259, 644
BstYI RGATCY 2 cut(s) 259, 644
BstZI CGGCCG 1 cut(s) 183
BsuI GTATCC 1 cut(s) 939
BsuRI GGCC 3 cut(s) 116, 185, 194
BtgI CCRYGG 1 cut(s) 1254
BtgZI GCGATG 1 cut(s) 1202
BtrI CACGTC 1 cut(s) 190
BtsCI GGATG 4 cut(s) 309, 1114, 1225, 1421
BveI ACCTGC 2 cut(s) 84, 1431
Cac8I GCNNGC 4 cut(s) 235, 270, 550, 1170
CfoI GCGC 6 cut(s) 22, 233, 377, 413, 542, 814
Cfr10I RCCGGY 1 cut(s) 181
CseI GACGC 2 cut(s) 296, 710
Csp6I GTAC 6 cut(s) 600, 979, 1131, 1342, 1348, 1399
CspCI CAANNNNNGTGG 2 cut(s) 1243, 1278
CviAII CATG 2 cut(s) 175, 588
CviQI GTAC 6 cut(s) 600, 979, 1131, 1342, 1348, 1399
DdeI CTNAG 3 cut(s) 840, 948, 1006
DpnI GATC 2 cut(s) 261, 646
DpnII GATC 2 cut(s) 259, 644
EaeI YGGCCR 2 cut(s) 183, 192
EagI CGGCCG 1 cut(s) 183
Eam1104I CTCTTC 4 cut(s) 252, 481, 915, 1326
EarI CTCTTC 4 cut(s) 252, 481, 915, 1326
EclXI CGGCCG 1 cut(s) 183
Eco130I CCWWGG 1 cut(s) 1311
Eco147I AGGCCT 1 cut(s) 116
Eco24I GRGCYC 1 cut(s) 150
Eco31I GGTCTC 1 cut(s) 333
Eco32I GATATC 1 cut(s) 1337
Eco52I CGGCCG 1 cut(s) 183
Eco91I GGTNACC 1 cut(s) 394
EcoO65I GGTNACC 1 cut(s) 394
EcoRII CCWGG 4 cut(s) 111, 201, 342, 1377
EcoRV GATATC 1 cut(s) 1337
EcoT14I CCWWGG 1 cut(s) 1311
EcoT22I ATGCAT 1 cut(s) 533
EcoT38I GRGCYC 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 1311
FaeI CATG 2 cut(s) 178, 591
FalI AAGNNNNNCTT 2 cut(s) 440, 472
FaqI GGGAC 1 cut(s) 711
FatI CATG 2 cut(s) 174, 587
FblI GTMKAC 2 cut(s) 72, 688
Fnu4HI GCNGC 6 cut(s) 159, 804, 835, 1287, 1407, 1438
FokI GGATG 4 cut(s) 296, 1121, 1212, 1428
FriOI GRGCYC 1 cut(s) 150
Fsp4HI GCNGC 6 cut(s) 159, 804, 835, 1287, 1407, 1438
FspBI CTAG 3 cut(s) 440, 516, 1434
GlaI GCGC 6 cut(s) 21, 232, 376, 412, 541, 813
GluI GCNGC 6 cut(s) 159, 804, 835, 1287, 1407, 1438
GsaI CCCAGC 2 cut(s) 698, 1185
HaeIII GGCC 3 cut(s) 116, 185, 194
HapII CCGG 1 cut(s) 182
HgaI GACGC 2 cut(s) 296, 710
HhaI GCGC 6 cut(s) 22, 233, 377, 413, 542, 814
Hin1I GRCGYC 2 cut(s) 288, 721
Hin1II CATG 2 cut(s) 178, 591
Hin6I GCGC 6 cut(s) 20, 231, 375, 411, 540, 812
HinP1I GCGC 6 cut(s) 20, 231, 375, 411, 540, 812
HinfI GANTC 4 cut(s) 426, 699, 1002, 1316
HpaII CCGG 1 cut(s) 182
HphI GGTGA 1 cut(s) 406
Hpy166II GTNNAC 4 cut(s) 73, 454, 689, 1399
Hpy188I TCNGA 4 cut(s) 252, 425, 580, 1230
Hpy188III TCNNGA 3 cut(s) 611, 821, 1084
Hpy8I GTNNAC 4 cut(s) 73, 454, 689, 1399
Hpy99I CGWCG 6 cut(s) 35, 104, 137, 140, 158, 191
HpyAV CCTTC 6 cut(s) 250, 325, 329, 442, 874, 1172
HpyCH4III ACNGT 4 cut(s) 463, 750, 970, 1135
HpyCH4IV ACGT 3 cut(s) 189, 575, 1391
HpyCH4V TGCA 9 cut(s) 48, 237, 531, 557, 566, 1145, 1172, 1265, 1367
HpyF3I CTNAG 3 cut(s) 840, 948, 1006
HpySE526I ACGT 3 cut(s) 189, 575, 1391
Hsp92I GRCGYC 2 cut(s) 288, 721
Hsp92II CATG 2 cut(s) 178, 591
HspAI GCGC 6 cut(s) 20, 231, 375, 411, 540, 812
Kzo9I GATC 2 cut(s) 259, 644
LmnI GCTCC 1 cut(s) 1256
Lsp1109I GCAGC 3 cut(s) 815, 1298, 1393
LweI GCATC 3 cut(s) 57, 799, 1182
MaeI CTAG 3 cut(s) 440, 516, 1434
MaeII ACGT 3 cut(s) 189, 575, 1391
MaeIII GTNAC 3 cut(s) 394, 744, 1304
MalI GATC 2 cut(s) 261, 646
MboI GATC 2 cut(s) 259, 644
MflI RGATCY 2 cut(s) 259, 644
MhlI GDGCHC 4 cut(s) 150, 297, 423, 554
MlsI TGGCCA 1 cut(s) 194
MluCI AATT 9 cut(s) 85, 128, 509, 893, 1205, 1221, 1239, 1329, 1362
MluNI TGGCCA 1 cut(s) 194
MlyI GAGTC 1 cut(s) 693
MmeI TCCRAC 2 cut(s) 337, 622
MnlI CCTC 8 cut(s) 26, 53, 482, 824, 916, 942, 1077, 1080
Mox20I TGGCCA 1 cut(s) 194
Mph1103I ATGCAT 1 cut(s) 533
MroXI GAANNNNTTC 1 cut(s) 1320
MscI TGGCCA 1 cut(s) 194
Msp20I TGGCCA 1 cut(s) 194
MspA1I CMGCKG 2 cut(s) 694, 806
MspI CCGG 1 cut(s) 182
MspR9I CCNGG 4 cut(s) 113, 203, 344, 1379
Mva1269I GAATGC 2 cut(s) 533, 1145
MvaI CCWGG 4 cut(s) 113, 203, 344, 1379
MvnI CGCG 4 cut(s) 20, 218, 233, 377
NdeII GATC 2 cut(s) 259, 644
NlaIII CATG 2 cut(s) 178, 591
NlaIV GGNNCC 3 cut(s) 470, 646, 1445
NmuCI GTSAC 3 cut(s) 394, 744, 1304
NsiI ATGCAT 1 cut(s) 533
PceI AGGCCT 1 cut(s) 116
PcsI WCGNNNNNNNCGW 1 cut(s) 294
PctI GAATGC 2 cut(s) 533, 1145
PdmI GAANNNNTTC 1 cut(s) 1320
PfeI GAWTC 3 cut(s) 426, 1002, 1316
PkrI GCNGC 6 cut(s) 160, 805, 836, 1288, 1408, 1439
PleI GAGTC 1 cut(s) 693
PpsI GAGTC 1 cut(s) 693
PsiI TTATAA 1 cut(s) 225
Psp6I CCWGG 4 cut(s) 111, 201, 342, 1377
PspEI GGTNACC 1 cut(s) 394
PspFI CCCAGC 2 cut(s) 694, 1181
PspGI CCWGG 4 cut(s) 111, 201, 342, 1377
PspN4I GGNNCC 3 cut(s) 470, 646, 1445
PsrI GAACNNNNNNTAC 4 cut(s) 101, 133, 1340, 1372
PsuI RGATCY 2 cut(s) 259, 644
PvuII CAGCTG 1 cut(s) 806
RsaI GTAC 6 cut(s) 601, 980, 1132, 1343, 1349, 1400
RsaNI GTAC 6 cut(s) 600, 979, 1131, 1342, 1348, 1399
SatI GCNGC 6 cut(s) 159, 804, 835, 1287, 1407, 1438
Sau3AI GATC 2 cut(s) 259, 644
SchI GAGTC 1 cut(s) 693
ScrFI CCNGG 4 cut(s) 113, 203, 344, 1379
SduI GDGCHC 4 cut(s) 150, 297, 423, 554
SfaNI GCATC 3 cut(s) 57, 799, 1182
SfiI GGCCNNNNNGGCC 1 cut(s) 191
SmlI CTYRAG 2 cut(s) 933, 1275
SmoI CTYRAG 2 cut(s) 933, 1275
Sse9I AATT 9 cut(s) 85, 128, 509, 893, 1205, 1221, 1239, 1329, 1362
SseBI AGGCCT 1 cut(s) 116
SsiI CCGC 8 cut(s) 159, 199, 392, 399, 692, 834, 1284, 1437
SspI AATATT 1 cut(s) 1299
SspMI CTAG 3 cut(s) 440, 516, 1434
StuI AGGCCT 1 cut(s) 116
StyD4I CCNGG 4 cut(s) 111, 201, 342, 1377
StyI CCWWGG 1 cut(s) 1311
TaaI ACNGT 4 cut(s) 463, 750, 970, 1135
TaiI ACGT 3 cut(s) 192, 578, 1394
TaqII GACCGA 1 cut(s) 732
TasI AATT 9 cut(s) 85, 128, 509, 893, 1205, 1221, 1239, 1329, 1362
TatI WGTACW 3 cut(s) 1341, 1347, 1398
TauI GCSGC 3 cut(s) 161, 837, 1440
TfiI GAWTC 3 cut(s) 426, 1002, 1316
TseFI GTSAC 3 cut(s) 394, 744, 1304
TseI GCWGC 3 cut(s) 803, 1286, 1406
Tsp45I GTSAC 3 cut(s) 394, 744, 1304
TspGWI ACGGA 3 cut(s) 142, 379, 637
XapI RAATTY 1 cut(s) 128
XmiI GTMKAC 2 cut(s) 72, 688
XmnI GAANNNNTTC 1 cut(s) 1320
XspI CTAG 3 cut(s) 440, 516, 1434
Zsp2I ATGCAT 1 cut(s) 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.