pycom12g17620
NAC Family

Probably inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0009915
Physical Location & Seq
Reverse (-)
20010851 .. 20014073
3223 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2898 bp
ATGGCGTTCGAGCTCTGTTTTCTCCTTCTTCTTGCTCCTCTTCTGGTACTCGCCCTAGACCCCGCTTTTAATGACGACGTTTTGGGGCTGATCGTCTTCAAGGCCGGGCTTTCTGACCCGGAAGCGAAGCTTGCTTCGTGGAGCGAGGACGACGGCAGTCCCTGCAACTGGGCCGGCGTCAAATGTGACCCGACAACCGGCAGAGTCGCCGAGGTTGTTCTTGATGGCTTTTCGCTGTCCGGGCACATCGGCCGCGGCCTTCTGCGGCTGCAGAACCTTCAAAAACTCTCGCTTTCCGACAACAACTTCACTGGGTTCATAAACCCTGATCTCCCACACCTCGGCAGCTTGCGGGTTATCGATTTGAGCCGGAACAGCCTATCTGGGTCGATCCCGGAGGAGTTCTTCAAGCAATGTGGATCCCTCAGGGTGGTTTCTTTCGCCGGAAACAACCTCACGGGGCGGATTCCGGAGTCCCTGAGCTTGTGCCAGACCTTGGTGGAGGTGAACTTTTCATCGAATTGGCTGTCTGGGAAATTGCCTACTGGGATTTGGTACTTGAGAATGCTTCAATCAATCGACTTGTCGGATAACTTGCTGGAGGGACAAGTTCCTGAAGATATGGAGAATTTGTATGATTTGAGAGTGATCAACTTGGGGGAAAACCGGTTTTCAGGACAGCTTCCAGGGAACATTGGAAGCTGTTTGCAGTTGAAGTTGCTTGATTTTAGTGATAATTTGTTTTCTGGGAGAATCCCGGATTCAATTCAGAGTCTCAATTCATGTACATCTCTGAGTTTACAGGGGAATTTTCTTGCGGGGCAAGTTCCGAATTGGCTTGGCGGTTTGAAAAACCTTGTGATGTTAGATGTGTCTGGAAATAATTTATCTGGTGAAATTCCAAGTTCGTTAGGACATCTAGAGGTGCTCGAAAAGTTGAATTTGTCTAGGAATGGATTTACTGGAAGCTTGCCTGATGCATTGGCAAACTGCATCAATCTGTTAGCTATAGATGTTAGCCATAATCTGTTGGCAGGTAAGCTTCCTTCGTGGATTTTTAAGCTTGGTCTGCGCAGTGTTTTGCTTTCTGGCAACCGATTAGGTGGAAGTGAGGAGTACAGTTCATTGGCGTCAATGGCAGCATCGAATGGAGGCCTGCAGGTCTTGGATTTGTCTTCGAACGCATTTTCTGATGTGCTTCCATCCGACATTGGTGTTCTTAGTAACTTGCAGTTCCTGAATATGTCTGGGAACCATCTGTTGGGTTGGATTCCAGCAAGTATAGGTAAATTAAAGGCGGCTTATGTTCTCGACTTGAGTGACAATTGGCTAAGTGGAAGCATTCCTGATGAAATCGGAGGGGCAATCTCACTCAAGGAACTAAGGCTGCAGAAGAACTTTCTAACCGGGAAGGTTCCAGCCGAAATTGTGAAGTGCTCATCTCTGACAAATCTGATTTTATCTCAGAACAATCTGACTGGCCCTATTCCTACTGCCATTGCTAACCTTACCAATCTTCAATATGTCGACTTGTCTTTGAACAAATTCTCAGGAGGCATACCGAAAGAGCTGACAAATCTTTCCCATCTCCTCTACTTCAATGTCTCCCACAACCACCTAGAGGGTGAACTACCACTAGGGGGATTCTTCAACACTATCCCACCCTCATCTGTCTTGGGCAATCCATCTCTTTGTGGATCTGCTGTTAACCGTGCCTGCCCTTCCGTCCACCCCAAACCCATTGTCCTCAACCCTAATTCTTCCAATCCCGTCGGTGGTTCTTCCGCTCCAACTCATGGTCACAAGATCATATTCAGCATATCAGCCCTCATTGCTATAGGCGCAGCTGTATTTATTGTCATTGGTGTCATAGCTGTAACCATCCTCAACATGCATGCGCGGTCTTCATTATCACTGTCTGCTGCTCCTCTTGAGTTATCAGGCGGGGAAGATTATAGTTGTTCCCCGACTAATGATCCAAACTACGGCAAGCTTGTCATGTTTTCTGGTGATGCTGACTTTGCTGCTGGGACACAAGCCTTGCTCAACAAGGACTGCGAACTTGGTCGTGGTGGGTTTGGAGTAGTTTACAAAACAGCCCTTCGTGATGGGTGTTCTGTTGCGATAAAAAAGCTATCAGTCTCAAGTTTGATCAAGTCCCAAGAAGTTTTCGAGAGGGAAGTTAAGGGACTTGGAAAGATCAGGCACCGTAATCTTGTGGCACTTGAAGGCTATTACTGGACTCCGTCCTTGCAGCTCATCATTTATGAGTACATACCATGCGGGAGTCTATTTAAGAATCTGCATGACGGACCTGGCAAAACCTGTCTCACTTGGCGGCAGAGGTTCCACATAATTCTTGGGATGGCAAAGGGTTTGGCTCATCTGCACCAAATGAACATAATCCACTACAATTTAAAATCAACTAATGTTTTGATAGACAACAATGGTGAGGCTAAGGTGGGAGACATTGGCCTGGCAAGGCTGTTGCCAACACTAGACCGGTGCATCTTAAGCAGCAAAATCCAAAGCGCACTTGGATACATGGCGCCCGAGTTTGCATGTCAAACGGTTAAGATTACTGAGAAATGTGATGTCTACGGATTCGGGATCTTGGTTTTGGAGGTGGTGACTGGAAAGAGGCCTGTCGAATATATGGAGGATGATGTGATAGTACTCTGTGATATGGTGAGGGGAGCGCTGGAGGAAGGCCGGGTGGAGGAATGTCTGGACAAAAACCTCCTTGGCAACTTTCCAGCAGAGGAGGCAATTCCGGTGATCAAGCTGGGTTTGATATGCGCGTCTCAAGTGCCATCAAATCGCCCGGACATGAATGAAGCGATCAACATTTTAGAACTCATCCAATGCCCTTCAGAAGGCCAGGAGGAATTAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

966

Amino Acids

103.78

Weight (kDa)

5.32

Isoelectric Point (pI)

36.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 24 - 63 1.8e-12 Leucine rich repeat N-terminal domain
LRR_14 PF23598 189 - 335 6e-09 Leucine-rich repeat region
LRR_8 PF13855 263 - 319 2e-06 Leucine rich repeat
LRR_14 PF23598 386 - 484 9.5e-06 Leucine-rich repeat region
LRR_8 PF13855 481 - 540 5.9e-07 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 684 - 897 3.5e-40 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 685 - 890 1.9e-39 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1073
Acc36I ACCTGC 2 cut(s) 1025, 1150
AccB1I GGYRCC 2 cut(s) 2205, 2548
AccB7I CCANNNNNTGG 3 cut(s) 496, 1261, 1796
AccBSI CCGCTC 1 cut(s) 1787
AccI GTMKAC 2 cut(s) 1527, 2598
AccII CGCG 3 cut(s) 255, 1900, 2801
AccIII TCCGGA 1 cut(s) 469
AclWI GGATC 6 cut(s) 385, 414, 427, 1705, 1970, 2618
AcoI YGGCCR 1 cut(s) 250
AcsI RAATTY 5 cut(s) 628, 808, 897, 940, 1544
AcuI CTGAAG 2 cut(s) 636, 2856
AcyI GRCGYC 3 cut(s) 177, 1130, 2549
AfaI GTAC 6 cut(s) 48, 557, 787, 1118, 2273, 2676
AfeI AGCGCT 1 cut(s) 2700
AflII CTTAAG 1 cut(s) 2512
AgeI ACCGGT 2 cut(s) 666, 2502
AjnI CCWGG 4 cut(s) 685, 2314, 2475, 2880
AjuI GAANNNNNNNTTGG 2 cut(s) 1244, 1276
Alw21I GWGCWC 3 cut(s) 15, 930, 1439
Alw26I GTCTC 6 cut(s) 779, 1609, 2146, 2333, 2460, 2808
AlwI GGATC 6 cut(s) 385, 414, 427, 1705, 1970, 2618
AlwNI CAGNNNCTG 2 cut(s) 162, 1237
Ama87I CYCGRG 1 cut(s) 2552
Aor13HI TCCGGA 1 cut(s) 469
Aor51HI AGCGCT 1 cut(s) 2700
ApeKI GCWGC 9 cut(s) 268, 345, 1139, 1387, 1844, 1922, 2024, 2254, 2517
ApoI RAATTY 5 cut(s) 628, 808, 897, 940, 1544
ArsI GACNNNNNNTTYG 4 cut(s) 512, 544, 1728, 1760
AsiGI ACCGGT 2 cut(s) 666, 2502
Asp700I GAANNNNTTC 1 cut(s) 1544
AspLEI GCGC 7 cut(s) 1074, 1844, 1900, 2534, 2551, 2701, 2801
AspS9I GGNCC 3 cut(s) 171, 1481, 2312
AsuC2I CCSGG 8 cut(s) 106, 119, 241, 395, 758, 1408, 2714, 2825
AsuHPI GGTGA 8 cut(s) 517, 905, 1637, 2021, 2462, 2641, 2701, 2788
AsuII TTCGAA 1 cut(s) 1178
AvaI CYCGRG 1 cut(s) 2552
AvaII GGWCC 1 cut(s) 2312
AxyI CCTNAGG 1 cut(s) 425
BaeGI GKGCMC 1 cut(s) 246
BamHI GGATCC 1 cut(s) 419
BanI GGYRCC 2 cut(s) 2205, 2548
BanII GRGCYC 1 cut(s) 15
BarI GAAGNNNNNNTAC 2 cut(s) 1030, 1062
BbsI GAAGAC 3 cut(s) 88, 1167, 1896
Bbv12I GWGCWC 3 cut(s) 15, 930, 1439
BbvI GCAGC 9 cut(s) 255, 357, 1151, 1374, 1856, 1909, 2011, 2266, 2529
BccI CCATC 9 cut(s) 218, 1210, 1263, 1593, 1693, 1889, 2102, 2359, 2821
BceAI ACGGC 2 cut(s) 169, 2002
BciT130I CCWGG 4 cut(s) 687, 2316, 2477, 2882
BciVI GTATCC 1 cut(s) 2534
BclI TGATCA 3 cut(s) 648, 2151, 2778
BcnI CCSGG 8 cut(s) 106, 119, 241, 395, 758, 1408, 2714, 2825
BcoDI GTCTC 6 cut(s) 779, 1609, 2146, 2333, 2460, 2808
BfaI CTAG 6 cut(s) 56, 920, 948, 1619, 1637, 2498
BfmI CTRYAG 5 cut(s) 269, 1008, 1157, 1388, 1836
BfoI RGCGCY 2 cut(s) 2552, 2702
BfrI CTTAAG 1 cut(s) 2512
BfuAI ACCTGC 2 cut(s) 1025, 1150
BfuI GTATCC 1 cut(s) 2534
BmcAI AGTACT 1 cut(s) 2676
Bme18I GGWCC 1 cut(s) 2312
BmeT110I CYCGRG 1 cut(s) 2552
BmgT120I GGNCC 3 cut(s) 171, 1481, 2312
BmiI GGNNCC 6 cut(s) 421, 1253, 1416, 2207, 2348, 2550
BmrI ACTGGG 3 cut(s) 178, 321, 555
BmsI GCATC 5 cut(s) 967, 1002, 1151, 2002, 2517
BmuI ACTGGG 3 cut(s) 178, 321, 555
BoxI GACNNNNGTC 1 cut(s) 156
BpiI GAAGAC 3 cut(s) 88, 1167, 1896
BpmI CTGGAG 2 cut(s) 620, 2723
Bpu10I CCTNAGC 2 cut(s) 479, 2457
Bpu14I TTCGAA 1 cut(s) 1178
BpuEI CTTGAG 6 cut(s) 580, 1336, 1358, 1952, 2128, 2790
BpuMI CCSGG 8 cut(s) 106, 119, 241, 395, 758, 1408, 2714, 2825
Bsa29I ATCGAT 1 cut(s) 360
BsaHI GRCGYC 3 cut(s) 177, 1130, 2549
BsaJI CCNNGG 6 cut(s) 210, 253, 340, 495, 686, 2743
BsaWI WCCGGW 4 cut(s) 469, 666, 2502, 2773
BsaXI ACNNNNNCTCC 2 cut(s) 617, 647
Bse118I RCCGGY 4 cut(s) 173, 197, 666, 2502
Bse1I ACTGG 7 cut(s) 173, 316, 550, 967, 1483, 2243, 2638
Bse21I CCTNAGG 1 cut(s) 425
Bse3DI GCAATG 3 cut(s) 419, 1497, 1830
BseAI TCCGGA 1 cut(s) 469
BseBI CCWGG 4 cut(s) 687, 2316, 2477, 2882
BseCI ATCGAT 1 cut(s) 360
BseDI CCNNGG 6 cut(s) 210, 253, 340, 495, 686, 2743
BseGI GGATG 5 cut(s) 1202, 1881, 2370, 2668, 2859
BseMI GCAATG 3 cut(s) 419, 1497, 1830
BseMII CTCAG 6 cut(s) 439, 470, 785, 1478, 1563, 2574
BseNI ACTGG 7 cut(s) 173, 316, 550, 967, 1483, 2243, 2638
BseRI GAGGAG 6 cut(s) 27, 413, 1127, 1580, 1917, 2777
BseSI GKGCMC 1 cut(s) 246
BseX3I CGGCCG 1 cut(s) 250
BseXI GCAGC 9 cut(s) 255, 357, 1151, 1374, 1856, 1909, 2011, 2266, 2529
BseYI CCCAGC 2 cut(s) 2027, 2785
BsgI GTGCAG 1 cut(s) 2372
Bsh1236I CGCG 3 cut(s) 255, 1900, 2801
Bsh1285I CGRYCG 1 cut(s) 253
BshNI GGYRCC 2 cut(s) 2205, 2548
BshTI ACCGGT 2 cut(s) 666, 2502
BshVI ATCGAT 1 cut(s) 360
BsiEI CGRYCG 1 cut(s) 253
BsiHKAI GWGCWC 3 cut(s) 15, 930, 1439
BsiHKCI CYCGRG 1 cut(s) 2552
BslFI GGGAC 6 cut(s) 144, 460, 618, 2044, 2143, 2202
BsmAI GTCTC 6 cut(s) 779, 1609, 2146, 2333, 2460, 2808
BsmBI CGTCTC 1 cut(s) 2808
BsmFI GGGAC 6 cut(s) 144, 460, 618, 2044, 2143, 2202
BsmI GAATGC 2 cut(s) 570, 1341
BsoBI CYCGRG 1 cut(s) 2552
Bsp119I TTCGAA 1 cut(s) 1178
Bsp1286I GDGCHC 4 cut(s) 15, 246, 930, 1439
Bsp13I TCCGGA 1 cut(s) 469
Bsp1407I TGTACA 1 cut(s) 785
BspCNI CTCAG 6 cut(s) 438, 471, 786, 1477, 1562, 2575
BspDI ATCGAT 1 cut(s) 360
BspEI TCCGGA 1 cut(s) 469
BspFNI CGCG 3 cut(s) 255, 1900, 2801
BspLI GGNNCC 6 cut(s) 421, 1253, 1416, 2207, 2348, 2550
BspMAI CTGCAG 3 cut(s) 273, 1161, 1392
BspMI ACCTGC 2 cut(s) 1025, 1150
BspPI GGATC 6 cut(s) 385, 414, 427, 1705, 1970, 2618
BspT104I TTCGAA 1 cut(s) 1178
BspT107I GGYRCC 2 cut(s) 2205, 2548
BspTI CTTAAG 1 cut(s) 2512
BsrBI CCGCTC 1 cut(s) 1787
BsrDI GCAATG 3 cut(s) 419, 1497, 1830
BsrFI RCCGGY 4 cut(s) 173, 197, 666, 2502
BsrGI TGTACA 1 cut(s) 785
BsrI ACTGG 7 cut(s) 173, 316, 550, 967, 1483, 2243, 2638
BssAI RCCGGY 4 cut(s) 173, 197, 666, 2502
BssECI CCNNGG 6 cut(s) 210, 253, 340, 495, 686, 2743
BssNI GRCGYC 3 cut(s) 177, 1130, 2549
BssT1I CCWWGG 2 cut(s) 495, 2743
Bst2UI CCWGG 4 cut(s) 687, 2316, 2477, 2882
Bst4CI ACNGT 5 cut(s) 1121, 1712, 1917, 2210, 2572
Bst6I CTCTTC 1 cut(s) 45
BstACI GRCGYC 3 cut(s) 177, 1130, 2549
BstAFI CTTAAG 1 cut(s) 2512
BstAPI GCANNNNNTGC 1 cut(s) 162
BstAUI TGTACA 1 cut(s) 785
BstBI TTCGAA 1 cut(s) 1178
BstC8I GCNNGC 8 cut(s) 132, 175, 350, 971, 1157, 1717, 1896, 1991
BstDSI CCRYGG 1 cut(s) 253
BstENI CCTNNNNNAGG 1 cut(s) 2874
BstF5I GGATG 5 cut(s) 1202, 1881, 2370, 2668, 2859
BstFNI CGCG 3 cut(s) 255, 1900, 2801
BstH2I RGCGCY 2 cut(s) 2552, 2702
BstHHI GCGC 7 cut(s) 1074, 1844, 1900, 2534, 2551, 2701, 2801
BstMAI GTCTC 6 cut(s) 779, 1609, 2146, 2333, 2460, 2808
BstMCI CGRYCG 1 cut(s) 253
BstNI CCWGG 4 cut(s) 687, 2316, 2477, 2882
BstNSI RCATGY 3 cut(s) 1894, 1898, 2565
BstPAI GACNNNNGTC 1 cut(s) 156
BstSFI CTRYAG 5 cut(s) 269, 1008, 1157, 1388, 1836
BstSLI GKGCMC 1 cut(s) 246
BstUI CGCG 3 cut(s) 255, 1900, 2801
BstV1I GCAGC 9 cut(s) 255, 357, 1151, 1374, 1856, 1909, 2011, 2266, 2529
BstV2I GAAGAC 3 cut(s) 88, 1167, 1896
BstX2I RGATCY 3 cut(s) 419, 1697, 2610
BstYI RGATCY 3 cut(s) 419, 1697, 2610
BstZI CGGCCG 1 cut(s) 250
Bsu15I ATCGAT 1 cut(s) 360
Bsu36I CCTNAGG 1 cut(s) 425
BsuI GTATCC 1 cut(s) 2534
BsuTUI ATCGAT 1 cut(s) 360
BtgI CCRYGG 1 cut(s) 253
BtsCI GGATG 5 cut(s) 1202, 1881, 2370, 2668, 2859
BtsI GCAGTG 1 cut(s) 1081
BtsIMutI CAGTG 3 cut(s) 309, 1081, 1913
BveI ACCTGC 2 cut(s) 1025, 1150
Cac8I GCNNGC 8 cut(s) 132, 175, 350, 971, 1157, 1717, 1896, 1991
CaiI CAGNNNCTG 2 cut(s) 162, 1237
CfoI GCGC 7 cut(s) 1074, 1844, 1900, 2534, 2551, 2701, 2801
Cfr10I RCCGGY 4 cut(s) 173, 197, 666, 2502
Cfr13I GGNCC 3 cut(s) 171, 1481, 2312
Cfr42I CCGCGG 1 cut(s) 256
ClaI ATCGAT 1 cut(s) 360
CseI GACGC 3 cut(s) 166, 1119, 2790
Csp6I GTAC 6 cut(s) 47, 556, 786, 1117, 2272, 2675
CspAI ACCGGT 2 cut(s) 666, 2502
CspCI CAANNNNNGTGG 2 cut(s) 397, 432
CviQI GTAC 6 cut(s) 47, 556, 786, 1117, 2272, 2675
DinI GGCGCC 1 cut(s) 2550
DraI TTTAAA 1 cut(s) 2418
EaeI YGGCCR 1 cut(s) 250
EagI CGGCCG 1 cut(s) 250
Eam1104I CTCTTC 1 cut(s) 45
EarI CTCTTC 1 cut(s) 45
EciI GGCGGA 1 cut(s) 478
Ecl136II GAGCTC 1 cut(s) 13
EclXI CGGCCG 1 cut(s) 250
Eco130I CCWWGG 2 cut(s) 495, 2743
Eco147I AGGCCT 2 cut(s) 1155, 2644
Eco24I GRGCYC 1 cut(s) 15
Eco47I GGWCC 1 cut(s) 2312
Eco47III AGCGCT 1 cut(s) 2700
Eco52I CGGCCG 1 cut(s) 250
Eco53kI GAGCTC 1 cut(s) 13
Eco57I CTGAAG 2 cut(s) 636, 2856
Eco81I CCTNAGG 1 cut(s) 425
Eco88I CYCGRG 1 cut(s) 2552
EcoICRI GAGCTC 1 cut(s) 13
EcoNI CCTNNNNNAGG 1 cut(s) 2874
EcoRII CCWGG 4 cut(s) 685, 2314, 2475, 2880
EcoT14I CCWWGG 2 cut(s) 495, 2743
EcoT22I ATGCAT 2 cut(s) 982, 1896
EcoT38I GRGCYC 1 cut(s) 15
EgeI GGCGCC 1 cut(s) 2550
EheI GGCGCC 1 cut(s) 2550
ErhI CCWWGG 2 cut(s) 495, 2743
Esp3I CGTCTC 1 cut(s) 2808
FalI AAGNNNNNCTT 2 cut(s) 114, 146
FaqI GGGAC 6 cut(s) 144, 460, 618, 2044, 2143, 2202
FauI CCCGC 5 cut(s) 70, 345, 811, 1937, 2276
FbaI TGATCA 3 cut(s) 648, 2151, 2778
FblI GTMKAC 2 cut(s) 1527, 2598
FokI GGATG 5 cut(s) 1189, 1868, 2377, 2675, 2846
FriOI GRGCYC 1 cut(s) 15
FspBI CTAG 6 cut(s) 56, 920, 948, 1619, 1637, 2498
FspI TGCGCA 1 cut(s) 1073
GlaI GCGC 7 cut(s) 1073, 1843, 1899, 2533, 2550, 2700, 2800
GsaI CCCAGC 2 cut(s) 2031, 2789
GsuI CTGGAG 2 cut(s) 620, 2723
HaeII RGCGCY 2 cut(s) 2552, 2702
HgaI GACGC 3 cut(s) 166, 1119, 2790
HhaI GCGC 7 cut(s) 1074, 1844, 1900, 2534, 2551, 2701, 2801
Hin1I GRCGYC 3 cut(s) 177, 1130, 2549
Hin6I GCGC 7 cut(s) 1072, 1842, 1898, 2532, 2549, 2699, 2799
HinP1I GCGC 7 cut(s) 1072, 1842, 1898, 2532, 2549, 2699, 2799
HincII GTYRAC 2 cut(s) 1528, 1708
HindII GTYRAC 2 cut(s) 1528, 1708
HindIII AAGCTT 5 cut(s) 128, 967, 1040, 1061, 1991
HpaI GTTAAC 1 cut(s) 1708
HphI GGTGA 8 cut(s) 517, 905, 1637, 2021, 2462, 2641, 2701, 2788
Hpy166II GTNNAC 8 cut(s) 508, 800, 1528, 1628, 1708, 1729, 2089, 2599
Hpy8I GTNNAC 8 cut(s) 508, 800, 1528, 1628, 1708, 1729, 2089, 2599
Hpy99I CGWCG 3 cut(s) 80, 155, 1775
HpyCH4III ACNGT 5 cut(s) 1121, 1712, 1917, 2210, 2572
HpyCH4IV ACGT 1 cut(s) 78
HpySE526I ACGT 1 cut(s) 78
Hsp92I GRCGYC 3 cut(s) 177, 1130, 2549
HspAI GCGC 7 cut(s) 1072, 1842, 1898, 2532, 2549, 2699, 2799
KasI GGCGCC 1 cut(s) 2548
Kpn2I TCCGGA 1 cut(s) 469
KroI GCCGGC 1 cut(s) 173
KroNI GCCGGC 1 cut(s) 175
Ksp22I TGATCA 3 cut(s) 648, 2151, 2778
KspAI GTTAAC 1 cut(s) 1708
KspI CCGCGG 1 cut(s) 256
LmnI GCTCC 5 cut(s) 40, 141, 1792, 1930, 2696
Lsp1109I GCAGC 9 cut(s) 255, 357, 1151, 1374, 1856, 1909, 2011, 2266, 2529
LweI GCATC 5 cut(s) 967, 1002, 1151, 2002, 2517
MaeI CTAG 6 cut(s) 56, 920, 948, 1619, 1637, 2498
MaeII ACGT 1 cut(s) 78
MaeIII GTNAC 6 cut(s) 185, 1223, 1319, 1799, 1876, 2629
MbiI CCGCTC 1 cut(s) 1787
MfeI CAATTG 1 cut(s) 1324
MflI RGATCY 3 cut(s) 419, 1697, 2610
MhlI GDGCHC 4 cut(s) 15, 246, 930, 1439
Mly113I GGCGCC 1 cut(s) 2549
MlyI GAGTC 5 cut(s) 213, 482, 781, 2236, 2296
MmeI TCCRAC 5 cut(s) 321, 567, 1230, 1247, 1814
Mph1103I ATGCAT 2 cut(s) 982, 1896
MroI TCCGGA 1 cut(s) 469
MroNI GCCGGC 1 cut(s) 173
MroXI GAANNNNTTC 1 cut(s) 1544
MseI TTAA 9 cut(s) 69, 1059, 1292, 1707, 2184, 2295, 2417, 2513, 2574
MspA1I CMGCKG 2 cut(s) 255, 1847
MspCI CTTAAG 1 cut(s) 2512
MunI CAATTG 1 cut(s) 1324
Mva1269I GAATGC 2 cut(s) 570, 1341
MvaI CCWGG 4 cut(s) 687, 2316, 2477, 2882
MvnI CGCG 3 cut(s) 255, 1900, 2801
NaeI GCCGGC 1 cut(s) 175
NarI GGCGCC 1 cut(s) 2549
NciI CCSGG 8 cut(s) 106, 119, 241, 395, 758, 1408, 2714, 2825
NgoMIV GCCGGC 1 cut(s) 173
NlaIV GGNNCC 6 cut(s) 421, 1253, 1416, 2207, 2348, 2550
NmeAIII GCCGAG 2 cut(s) 235, 321
NmuCI GTSAC 4 cut(s) 185, 1319, 1799, 2629
NsbI TGCGCA 1 cut(s) 1073
NsiI ATGCAT 2 cut(s) 982, 1896
NspI RCATGY 3 cut(s) 1894, 1898, 2565
NspV TTCGAA 1 cut(s) 1178
PaeI GCATGC 1 cut(s) 1898
PceI AGGCCT 2 cut(s) 1155, 2644
PctI GAATGC 2 cut(s) 570, 1341
PdiI GCCGGC 1 cut(s) 175
PdmI GAANNNNTTC 1 cut(s) 1544
PfeI GAWTC 7 cut(s) 466, 753, 761, 1270, 1644, 2299, 2604
PflFI GACNNNGTC 1 cut(s) 2245
PflMI CCANNNNNTGG 3 cut(s) 496, 1261, 1796
PfoI TCCNGGA 2 cut(s) 393, 756
PinAI ACCGGT 2 cut(s) 666, 2502
PleI GAGTC 5 cut(s) 212, 481, 780, 2236, 2295
PluTI GGCGCC 1 cut(s) 2552
PpsI GAGTC 5 cut(s) 212, 481, 780, 2236, 2295
PshAI GACNNNNGTC 1 cut(s) 156
Psp124BI GAGCTC 1 cut(s) 15
Psp6I CCWGG 4 cut(s) 685, 2314, 2475, 2880
PspFI CCCAGC 2 cut(s) 2027, 2785
PspGI CCWGG 4 cut(s) 685, 2314, 2475, 2880
PspN4I GGNNCC 6 cut(s) 421, 1253, 1416, 2207, 2348, 2550
PspPI GGNCC 3 cut(s) 171, 1481, 2312
PstI CTGCAG 3 cut(s) 273, 1161, 1392
PstNI CAGNNNCTG 2 cut(s) 162, 1237
PsuI RGATCY 3 cut(s) 419, 1697, 2610
PsyI GACNNNGTC 1 cut(s) 2245
PvuII CAGCTG 1 cut(s) 1847
RsaI GTAC 6 cut(s) 48, 557, 787, 1118, 2273, 2676
RsaNI GTAC 6 cut(s) 47, 556, 786, 1117, 2272, 2675
SacI GAGCTC 1 cut(s) 15
SacII CCGCGG 1 cut(s) 256
SalI GTCGAC 1 cut(s) 1526
SaqAI TTAA 9 cut(s) 69, 1059, 1292, 1707, 2184, 2295, 2417, 2513, 2574
Sau96I GGNCC 3 cut(s) 171, 1481, 2312
SbfI CCTGCAGG 1 cut(s) 1161
ScaI AGTACT 1 cut(s) 2676
SchI GAGTC 5 cut(s) 213, 482, 781, 2236, 2296
SdaI CCTGCAGG 1 cut(s) 1161
SduI GDGCHC 4 cut(s) 15, 246, 930, 1439
SfaNI GCATC 5 cut(s) 967, 1002, 1151, 2002, 2517
SfcI CTRYAG 5 cut(s) 269, 1008, 1157, 1388, 1836
SfoI GGCGCC 1 cut(s) 2550
Sfr303I CCGCGG 1 cut(s) 256
SfuI TTCGAA 1 cut(s) 1178
SgrBI CCGCGG 1 cut(s) 256
SinI GGWCC 1 cut(s) 2312
SmlI CTYRAG 7 cut(s) 559, 1315, 1373, 1931, 2143, 2512, 2805
SmoI CTYRAG 7 cut(s) 559, 1315, 1373, 1931, 2143, 2512, 2805
SphI GCATGC 1 cut(s) 1898
Sse8387I CCTGCAGG 1 cut(s) 1161
SseBI AGGCCT 2 cut(s) 1155, 2644
SspDI GGCGCC 1 cut(s) 2548
SspMI CTAG 6 cut(s) 56, 920, 948, 1619, 1637, 2498
SstI GAGCTC 1 cut(s) 15
StuI AGGCCT 2 cut(s) 1155, 2644
StyI CCWWGG 2 cut(s) 495, 2743
TaaI ACNGT 5 cut(s) 1121, 1712, 1917, 2210, 2572
TaiI ACGT 1 cut(s) 81
TatI WGTACW 4 cut(s) 785, 1116, 2271, 2674
TauI GCSGC 5 cut(s) 255, 258, 268, 1301, 2341
TfiI GAWTC 7 cut(s) 466, 753, 761, 1270, 1644, 2299, 2604
Tru1I TTAA 9 cut(s) 69, 1059, 1292, 1707, 2184, 2295, 2417, 2513, 2574
Tru9I TTAA 9 cut(s) 69, 1059, 1292, 1707, 2184, 2295, 2417, 2513, 2574
TscAI CASTG 3 cut(s) 316, 1081, 1920
TseFI GTSAC 4 cut(s) 185, 1319, 1799, 2629
TseI GCWGC 9 cut(s) 268, 345, 1139, 1387, 1844, 1922, 2024, 2254, 2517
Tsp45I GTSAC 4 cut(s) 185, 1319, 1799, 2629
TspDTI ATGAA 9 cut(s) 307, 504, 771, 1113, 1365, 1896, 2411, 2846, 2850
TspGWI ACGGA 4 cut(s) 1714, 2235, 2325, 2616
TspRI CASTG 3 cut(s) 316, 1081, 1920
Tth111I GACNNNGTC 1 cut(s) 2245
Van91I CCANNNNNTGG 3 cut(s) 496, 1261, 1796
Vha464I CTTAAG 1 cut(s) 2512
VpaK11BI GGWCC 1 cut(s) 2312
XagI CCTNNNNNAGG 1 cut(s) 2874
XapI RAATTY 5 cut(s) 628, 808, 897, 940, 1544
XbaI TCTAGA 1 cut(s) 919
XceI RCATGY 3 cut(s) 1894, 1898, 2565
XcmI CCANNNNNNNNNTGG 2 cut(s) 2357, 2534
XmiI GTMKAC 2 cut(s) 1527, 2598
XmnI GAANNNNTTC 1 cut(s) 1544
XspI CTAG 6 cut(s) 56, 920, 948, 1619, 1637, 2498
ZrmI AGTACT 1 cut(s) 2676
Zsp2I ATGCAT 2 cut(s) 982, 1896
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.