pycom12g19430

synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0009298
Physical Location & Seq
Forward (+)
21287966 .. 21289756
1791 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1059 bp
ATGCCCTTCCCTCGTCCTTTCCTCTCTCCCCAACCCGCCACTGTATTGTTCGCCCCCAAAGCCTTCGTCTTTCTCAGCCGCCGCCGAGCCACCATGTCAGCCCCACACGACGCCGCAAATCCCAGTTCCCAAGATGCGCTGCAAGCCCCAGGGAACTACCCCGTGCCCCTCTCCCCGCCTCTTCCCGCCATCTCAAAGCACATAGAGCTAGCGAGAGCCATGTCCGCCTCTTCCAAATCCAGCCTCTTCTCCCTCTCGAGAAACGACGTCGTCTACGAGGACGAGTGGCTCATTGTGGCCAACAAGCCTCAGGGTGTTTACTGTGAGTCCGTGTTGGAATCTGTCCCTCAGCTTCTCTGTGACTCGGCCGAGTCAGATGAGTCAGTTGCAGAGACCCGAACAAAGCAGCCAGAGCTCCATCTTGCAAATCGACTTGATCGCGACACCAGTGGATTGATGATAATAACCAAGTCACACAAAGTAGCGGCCAAGCTTGTGAAGGCATTTACAGATCACAAAGTTTCCAAAACATACATTGCACGCTGTCTTGGTTCAGCTCCGAAATGGGAAAGAATCACCCTCAAGTCAGGTCATGGTCGGTCAAAGTTTGGCGCATGGCGAGTGTATGCTGCTTCAGACGTAGGTCGGACACTTCCAGGTGGGTCAATGGTCAGAGACATGGAAACATCGTTTGAAATTTTATCAGTAAATGGACAAGGGAGCTACGAAGAGCCATCTGAGTTTAAGAAAGATGAAGCAAATGTTGTAGTGGTTGAAGAGAAAGCTGTAATAGATGGAGACGCAAAGAAGGACGAGATTTTAGTAAGAGCCCGTCCTAGGAGTGGACGAACACATCAAATTCGCTTGCATTGCCAGTATCTTGGAATTTCCATAAGAGGGGATGTCAAGTATGAGGGTGTGTATGAGTGGAAAGGGACAACTTATGACGGCCATGAACTTCATGCGGAGAGCTTGTCTCTTGAGCACCCTGTTACTGGTCTTCCAGTAATGTTTCGGGCACCTGTACCTTCTTGGGCCAGCCAGGCGTTGCAGCACTAG

Protein Analysis

353

Amino Acids

38.86

Weight (kDa)

8.24

Isoelectric Point (pI)

53.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PseudoU_synth_2 PF00849 97 - 292 6.5e-23 RNA pseudouridylate synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 270
AccB1I GGYRCC 1 cut(s) 1018
AccI GTMKAC 1 cut(s) 273
AccII CGCG 1 cut(s) 441
AciI CCGC 9 cut(s) 36, 79, 82, 114, 176, 186, 225, 485, 965
AcoI YGGCCR 4 cut(s) 297, 366, 486, 949
AcsI RAATTY 3 cut(s) 696, 858, 885
AcuI CTGAAG 1 cut(s) 618
AcyI GRCGYC 2 cut(s) 111, 267
AfaI GTAC 1 cut(s) 1026
AfiI CCNNNNNNNGG 4 cut(s) 837, 842, 897, 995
AgsI TTSAA 2 cut(s) 695, 776
AjnI CCWGG 3 cut(s) 148, 655, 1041
AluBI AGCT 8 cut(s) 208, 352, 415, 493, 557, 723, 785, 972
AluI AGCT 8 cut(s) 208, 352, 415, 493, 557, 723, 785, 972
Alw21I GWGCWC 2 cut(s) 417, 987
Alw26I GTCTC 4 cut(s) 386, 669, 792, 981
Ama87I CYCGRG 1 cut(s) 256
AoxI GGCC 5 cut(s) 297, 366, 486, 949, 1035
ApeKI GCWGC 4 cut(s) 139, 406, 629, 1051
ApoI RAATTY 3 cut(s) 696, 858, 885
ArsI GACNNNNNNTTYG 2 cut(s) 51, 83
AspA2I CCTAGG 1 cut(s) 836
AspLEI GCGC 2 cut(s) 139, 614
AspS9I GGNCC 1 cut(s) 1035
AsuHPI GGTGA 1 cut(s) 568
AsuNHI GCTAGC 1 cut(s) 208
AvaI CYCGRG 1 cut(s) 256
AvrII CCTAGG 1 cut(s) 836
AxyI CCTNAGG 1 cut(s) 309
BaeGI GKGCMC 2 cut(s) 168, 1021
BalI TGGCCA 1 cut(s) 299
BanI GGYRCC 1 cut(s) 1018
BanII GRGCYC 2 cut(s) 417, 832
BbsI GAAGAC 1 cut(s) 992
Bbv12I GWGCWC 2 cut(s) 417, 987
BbvCI CCTCAGC 1 cut(s) 348
BbvI GCAGC 3 cut(s) 126, 418, 616
BccI CCATC 4 cut(s) 197, 426, 742, 788
BceAI ACGGC 1 cut(s) 964
BciT130I CCWGG 3 cut(s) 150, 657, 1043
BcoDI GTCTC 4 cut(s) 386, 669, 792, 981
BfaI CTAG 3 cut(s) 209, 837, 1057
BglI GCCNNNNNGGC 1 cut(s) 1043
BisI GCNGC 8 cut(s) 79, 82, 114, 140, 407, 486, 630, 1052
BlnI CCTAGG 1 cut(s) 836
BlsI GCNGC 8 cut(s) 80, 83, 115, 141, 408, 487, 631, 1053
Bme1390I CCNGG 3 cut(s) 150, 657, 1043
BmeT110I CYCGRG 1 cut(s) 256
BmgT120I GGNCC 1 cut(s) 1035
BmiI GGNNCC 1 cut(s) 1020
BmrFI CCNGG 3 cut(s) 150, 657, 1043
BmrI ACTGGG 1 cut(s) 117
BmsI GCATC 1 cut(s) 124
BmtI GCTAGC 1 cut(s) 212
BmuI ACTGGG 1 cut(s) 117
BoxI GACNNNNGTC 1 cut(s) 642
BpiI GAAGAC 1 cut(s) 992
BplI GAGNNNNNCTC 2 cut(s) 961, 993
Bpu10I CCTNAGC 1 cut(s) 348
BpuEI CTTGAG 2 cut(s) 566, 1001
BsaHI GRCGYC 2 cut(s) 111, 267
BsaI GGTCTC 1 cut(s) 386
BsaJI CCNNGG 3 cut(s) 148, 149, 836
BsaXI ACNNNNNCTCC 2 cut(s) 959, 989
Bsc4I CCNNNNNNNGG 4 cut(s) 837, 842, 897, 995
Bse1I ACTGG 5 cut(s) 123, 447, 874, 1000, 1004
Bse21I CCTNAGG 1 cut(s) 309
Bse3DI GCAATG 2 cut(s) 534, 868
BseBI CCWGG 3 cut(s) 150, 657, 1043
BseDI CCNNGG 3 cut(s) 148, 149, 836
BseGI GGATG 1 cut(s) 907
BseLI CCNNNNNNNGG 4 cut(s) 837, 842, 897, 995
BseMI GCAATG 2 cut(s) 534, 868
BseMII CTCAG 4 cut(s) 88, 323, 362, 729
BseNI ACTGG 5 cut(s) 123, 447, 874, 1000, 1004
BseSI GKGCMC 2 cut(s) 168, 1021
BseX3I CGGCCG 1 cut(s) 366
BseXI GCAGC 3 cut(s) 126, 418, 616
Bsh1236I CGCG 1 cut(s) 441
Bsh1285I CGRYCG 1 cut(s) 369
BshFI GGCC 5 cut(s) 299, 368, 488, 951, 1037
BshNI GGYRCC 1 cut(s) 1018
BsiEI CGRYCG 1 cut(s) 369
BsiHKAI GWGCWC 2 cut(s) 417, 987
BsiHKCI CYCGRG 1 cut(s) 256
BslFI GGGAC 2 cut(s) 329, 949
BslI CCNNNNNNNGG 4 cut(s) 837, 842, 897, 995
BsmAI GTCTC 4 cut(s) 386, 669, 792, 981
BsmBI CGTCTC 1 cut(s) 792
BsmFI GGGAC 2 cut(s) 329, 949
BsnI GGCC 5 cut(s) 299, 368, 488, 951, 1037
Bso31I GGTCTC 1 cut(s) 386
BsoBI CYCGRG 1 cut(s) 256
Bsp1286I GDGCHC 5 cut(s) 168, 417, 832, 987, 1021
Bsp143I GATC 2 cut(s) 436, 511
Bsp68I TCGCGA 1 cut(s) 441
BspACI CCGC 9 cut(s) 36, 79, 82, 114, 176, 186, 225, 485, 965
BspANI GGCC 5 cut(s) 299, 368, 488, 951, 1037
BspCNI CTCAG 4 cut(s) 87, 322, 361, 730
BspFNI CGCG 1 cut(s) 441
BspLI GGNNCC 1 cut(s) 1020
BspOI GCTAGC 1 cut(s) 212
BspQI GCTCTTC 1 cut(s) 723
BspT107I GGYRCC 1 cut(s) 1018
BspTNI GGTCTC 1 cut(s) 386
BsrDI GCAATG 2 cut(s) 534, 868
BsrI ACTGG 5 cut(s) 123, 447, 874, 1000, 1004
BssECI CCNNGG 3 cut(s) 148, 149, 836
BssMI GATC 2 cut(s) 436, 511
BssNI GRCGYC 2 cut(s) 111, 267
BssT1I CCWWGG 1 cut(s) 836
Bst2UI CCWGG 3 cut(s) 150, 657, 1043
Bst4CI ACNGT 2 cut(s) 43, 323
Bst6I CTCTTC 5 cut(s) 186, 235, 251, 723, 771
BstACI GRCGYC 2 cut(s) 111, 267
BstC8I GCNNGC 5 cut(s) 144, 210, 541, 866, 1039
BstDEI CTNAG 4 cut(s) 74, 309, 348, 738
BstF5I GGATG 1 cut(s) 907
BstFNI CGCG 1 cut(s) 441
BstHHI GCGC 2 cut(s) 139, 614
BstKTI GATC 2 cut(s) 439, 514
BstMAI GTCTC 4 cut(s) 386, 669, 792, 981
BstMBI GATC 2 cut(s) 436, 511
BstMCI CGRYCG 1 cut(s) 369
BstMWI GCNNNNNNNGC 7 cut(s) 59, 143, 205, 224, 412, 870, 1043
BstNI CCWGG 3 cut(s) 150, 657, 1043
BstPAI GACNNNNGTC 1 cut(s) 642
BstSCI CCNGG 3 cut(s) 148, 655, 1041
BstSLI GKGCMC 2 cut(s) 168, 1021
BstUI CGCG 1 cut(s) 441
BstV1I GCAGC 3 cut(s) 126, 418, 616
BstV2I GAAGAC 1 cut(s) 992
BstXI CCANNNNNNTGG 1 cut(s) 881
BstZI CGGCCG 1 cut(s) 366
Bsu36I CCTNAGG 1 cut(s) 309
BsuRI GGCC 5 cut(s) 299, 368, 488, 951, 1037
BtsCI GGATG 1 cut(s) 907
BtsIMutI CAGTG 2 cut(s) 39, 454
BtuMI TCGCGA 1 cut(s) 441
Cac8I GCNNGC 5 cut(s) 144, 210, 541, 866, 1039
CfoI GCGC 2 cut(s) 139, 614
Cfr13I GGNCC 1 cut(s) 1035
CseI GACGC 2 cut(s) 119, 809
Csp6I GTAC 1 cut(s) 1025
CviAII CATG 7 cut(s) 94, 220, 593, 615, 679, 953, 962
CviQI GTAC 1 cut(s) 1025
DdeI CTNAG 4 cut(s) 74, 309, 348, 738
DpnI GATC 2 cut(s) 438, 513
DpnII GATC 2 cut(s) 436, 511
EaeI YGGCCR 4 cut(s) 297, 366, 486, 949
EagI CGGCCG 1 cut(s) 366
Eam1104I CTCTTC 5 cut(s) 186, 235, 251, 723, 771
EarI CTCTTC 5 cut(s) 186, 235, 251, 723, 771
EciI GGCGGA 1 cut(s) 214
Ecl136II GAGCTC 1 cut(s) 415
EclXI CGGCCG 1 cut(s) 366
Eco130I CCWWGG 1 cut(s) 836
Eco24I GRGCYC 2 cut(s) 417, 832
Eco31I GGTCTC 1 cut(s) 386
Eco52I CGGCCG 1 cut(s) 366
Eco53kI GAGCTC 1 cut(s) 415
Eco57I CTGAAG 1 cut(s) 618
Eco81I CCTNAGG 1 cut(s) 309
Eco88I CYCGRG 1 cut(s) 256
EcoICRI GAGCTC 1 cut(s) 415
EcoRII CCWGG 3 cut(s) 148, 655, 1041
EcoT14I CCWWGG 1 cut(s) 836
EcoT38I GRGCYC 2 cut(s) 417, 832
ErhI CCWWGG 1 cut(s) 836
Esp3I CGTCTC 1 cut(s) 792
FaeI CATG 7 cut(s) 97, 223, 596, 618, 682, 956, 965
FaqI GGGAC 2 cut(s) 329, 949
FatI CATG 7 cut(s) 93, 219, 592, 614, 678, 952, 961
FauI CCCGC 3 cut(s) 43, 183, 193
FblI GTMKAC 1 cut(s) 273
Fnu4HI GCNGC 8 cut(s) 79, 82, 114, 140, 407, 486, 630, 1052
FokI GGATG 1 cut(s) 914
FriOI GRGCYC 2 cut(s) 417, 832
Fsp4HI GCNGC 8 cut(s) 79, 82, 114, 140, 407, 486, 630, 1052
FspBI CTAG 3 cut(s) 209, 837, 1057
GlaI GCGC 2 cut(s) 138, 613
GluI GCNGC 8 cut(s) 79, 82, 114, 140, 407, 486, 630, 1052
HaeIII GGCC 5 cut(s) 299, 368, 488, 951, 1037
HgaI GACGC 2 cut(s) 119, 809
HhaI GCGC 2 cut(s) 139, 614
Hin1I GRCGYC 2 cut(s) 111, 267
Hin1II CATG 7 cut(s) 97, 223, 596, 618, 682, 956, 965
Hin6I GCGC 2 cut(s) 137, 612
HinP1I GCGC 2 cut(s) 137, 612
HindIII AAGCTT 1 cut(s) 491
HinfI GANTC 6 cut(s) 326, 338, 362, 371, 380, 573
HphI GGTGA 1 cut(s) 568
Hpy166II GTNNAC 3 cut(s) 274, 319, 845
Hpy188I TCNGA 6 cut(s) 376, 561, 637, 648, 674, 739
Hpy188III TCNNGA 4 cut(s) 256, 258, 440, 980
Hpy8I GTNNAC 3 cut(s) 274, 319, 845
Hpy99I CGWCG 3 cut(s) 113, 269, 272
HpyAV CCTTC 5 cut(s) 16, 73, 493, 802, 1038
HpyCH4III ACNGT 2 cut(s) 43, 323
HpyCH4IV ACGT 2 cut(s) 267, 639
HpyCH4V TGCA 6 cut(s) 142, 389, 425, 539, 868, 1051
HpyF10VI GCNNNNNNNGC 7 cut(s) 59, 143, 205, 224, 412, 870, 1043
HpyF3I CTNAG 4 cut(s) 74, 309, 348, 738
HpySE526I ACGT 2 cut(s) 267, 639
Hsp92I GRCGYC 2 cut(s) 111, 267
Hsp92II CATG 7 cut(s) 97, 223, 596, 618, 682, 956, 965
HspAI GCGC 2 cut(s) 137, 612
Kzo9I GATC 2 cut(s) 436, 511
LguI GCTCTTC 1 cut(s) 723
LmnI GCTCC 3 cut(s) 420, 562, 720
Lsp1109I GCAGC 3 cut(s) 126, 418, 616
LweI GCATC 1 cut(s) 124
MaeI CTAG 3 cut(s) 209, 837, 1057
MaeII ACGT 2 cut(s) 267, 639
MaeIII GTNAC 3 cut(s) 359, 471, 991
MalI GATC 2 cut(s) 438, 513
MboI GATC 2 cut(s) 436, 511
MboII GAAGA 6 cut(s) 173, 222, 238, 740, 788, 992
MhlI GDGCHC 5 cut(s) 168, 417, 832, 987, 1021
MlsI TGGCCA 1 cut(s) 299
MluCI AATT 3 cut(s) 696, 858, 885
MluNI TGGCCA 1 cut(s) 299
MlyI GAGTC 4 cut(s) 335, 356, 380, 389
MmeI TCCRAC 2 cut(s) 315, 626
Mox20I TGGCCA 1 cut(s) 299
MscI TGGCCA 1 cut(s) 299
MseI TTAA 1 cut(s) 744
Msp20I TGGCCA 1 cut(s) 299
MspR9I CCNGG 3 cut(s) 150, 657, 1043
MvaI CCWGG 3 cut(s) 150, 657, 1043
MvnI CGCG 1 cut(s) 441
MwoI GCNNNNNNNGC 7 cut(s) 59, 143, 205, 224, 412, 870, 1043
NdeII GATC 2 cut(s) 436, 511
NheI GCTAGC 1 cut(s) 208
NlaIII CATG 7 cut(s) 97, 223, 596, 618, 682, 956, 965
NlaIV GGNNCC 1 cut(s) 1020
NmeAIII GCCGAG 3 cut(s) 110, 344, 394
NmuCI GTSAC 2 cut(s) 359, 471
NruI TCGCGA 1 cut(s) 441
PaeR7I CTCGAG 1 cut(s) 256
PasI CCCWGGG 1 cut(s) 149
PciSI GCTCTTC 1 cut(s) 723
PcsI WCGNNNNNNNCGW 1 cut(s) 273
PfeI GAWTC 2 cut(s) 338, 573
PflFI GACNNNGTC 1 cut(s) 269
PkrI GCNGC 8 cut(s) 80, 83, 115, 141, 408, 487, 631, 1053
PleI GAGTC 4 cut(s) 334, 356, 379, 388
PpsI GAGTC 4 cut(s) 334, 356, 379, 388
PshAI GACNNNNGTC 1 cut(s) 642
Psp124BI GAGCTC 1 cut(s) 417
Psp6I CCWGG 3 cut(s) 148, 655, 1041
PspGI CCWGG 3 cut(s) 148, 655, 1041
PspN4I GGNNCC 1 cut(s) 1020
PspPI GGNCC 1 cut(s) 1035
PsyI GACNNNGTC 1 cut(s) 269
RruI TCGCGA 1 cut(s) 441
RsaI GTAC 1 cut(s) 1026
RsaNI GTAC 1 cut(s) 1025
SacI GAGCTC 1 cut(s) 417
SapI GCTCTTC 1 cut(s) 723
SaqAI TTAA 1 cut(s) 744
SatI GCNGC 8 cut(s) 79, 82, 114, 140, 407, 486, 630, 1052
Sau3AI GATC 2 cut(s) 436, 511
Sau96I GGNCC 1 cut(s) 1035
SchI GAGTC 4 cut(s) 335, 356, 380, 389
ScrFI CCNGG 3 cut(s) 150, 657, 1043
SduI GDGCHC 5 cut(s) 168, 417, 832, 987, 1021
SfaNI GCATC 1 cut(s) 124
Sfr274I CTCGAG 1 cut(s) 256
SlaI CTCGAG 1 cut(s) 256
SmlI CTYRAG 3 cut(s) 256, 581, 980
SmoI CTYRAG 3 cut(s) 256, 581, 980
Sse9I AATT 3 cut(s) 696, 858, 885
SsiI CCGC 9 cut(s) 36, 79, 82, 114, 176, 186, 225, 485, 965
SspMI CTAG 3 cut(s) 209, 837, 1057
SstI GAGCTC 1 cut(s) 417
StyD4I CCNGG 3 cut(s) 148, 655, 1041
StyI CCWWGG 1 cut(s) 836
TaaI ACNGT 2 cut(s) 43, 323
TaiI ACGT 2 cut(s) 270, 642
TaqI TCGA 2 cut(s) 257, 430
TaqII GACCGA 1 cut(s) 588
TasI AATT 3 cut(s) 696, 858, 885
TauI GCSGC 4 cut(s) 81, 84, 116, 488
TfiI GAWTC 2 cut(s) 338, 573
Tru1I TTAA 1 cut(s) 744
Tru9I TTAA 1 cut(s) 744
TscAI CASTG 2 cut(s) 46, 454
TseFI GTSAC 2 cut(s) 359, 471
TseI GCWGC 4 cut(s) 139, 406, 629, 1051
Tsp45I GTSAC 2 cut(s) 359, 471
TspDTI ATGAA 3 cut(s) 768, 950, 969
TspGWI ACGGA 1 cut(s) 319
TspRI CASTG 2 cut(s) 46, 454
Tth111I GACNNNGTC 1 cut(s) 269
XapI RAATTY 3 cut(s) 696, 858, 885
XhoI CTCGAG 1 cut(s) 256
XmaJI CCTAGG 1 cut(s) 836
XmiI GTMKAC 1 cut(s) 273
XspI CTAG 3 cut(s) 209, 837, 1057
ZraI GACGTC 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.