Rorug03G0018000

synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0009298
Physical Location & Seq
Reverse (-)
1437588 .. 1438504
917 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0018000.1

Sequence Viewer

Length: 828 bp
ATGCCAAAAAGTAGTCTGATAACTTGTGTTCTTATTTGCTTTGCAATTTATCATGATGATGCAAAGGCAGAGAAGCACTGTCGGCCAGCATCTTGTGGGGATCTCCATCACATTAGGTACCCGTTTCACTTGAAAAGTACTGGTGCTTCTGCTAACAATTGTCTGAACTCTCCAGCTGAACTTTTATGTGAGCAGAATCGCACCATTTTAAAACTTTACAATGGTACATATTATGTCATGGGAATCAACTATGAATTGGAGACGATCCAGGTTGTGGATCCTGGTCTACTAAGCGATACTTGCCCCTTTAGGCCGATTCACCGTTTGACAAGTGATAACTTTACCGTTGGCCTTGACGAAGAAGACTACGAACTATTTCTTTCCGGACCCGGCTTGGGTAATTATGGGTATGCAACCTTCTATGATTGCCAGTCGAAAGTTGAGTCCCCAGACTACATAAAAATTAGTTGCAGTAATAGCTCATCGTTGTCGTCACCAACTACATATTCTTACATTAAGCTGGGAGATAGTGTGTCTCATCTTCTACCATATTGTAGTATCACCACCATCATCCCTTCCATGTTATACGAATCTGGTAATGATGACCTTTCATTTTCTTTCATCCGCGATGAGTTGAGAAAGGGCTTTCAACTTGATTGGTTCAATCTTGTCCCACACAGTTACTGCTACAGCCGATTCCAGTCCCTCTATTGTTTTCGCAAATACTTGAAACAAGGCAAGTCTGACCTTTTCTGTTACTACTTGCTTTCAATTTTCCCAAACATGAACCTAACTCATTTCCTCTTTGTTGATTCTGTATTCACATAG

Protein Analysis

275

Amino Acids

31.37

Weight (kDa)

5.67

Isoelectric Point (pI)

36.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 27 - 93 1.7e-12 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 117
AccB1I GGYRCC 1 cut(s) 117
AccB7I CCANNNNNTGG 1 cut(s) 274
AccI GTMKAC 1 cut(s) 286
AccII CGCG 1 cut(s) 627
AccIII TCCGGA 1 cut(s) 383
AciI CCGC 1 cut(s) 625
AclWI GGATC 4 cut(s) 108, 259, 272, 285
AcoI YGGCCR 1 cut(s) 83
AfaI GTAC 3 cut(s) 119, 139, 226
AfiI CCNNNNNNNGG 2 cut(s) 274, 395
AgsI TTSAA 5 cut(s) 133, 650, 664, 730, 771
AjnI CCWGG 2 cut(s) 267, 280
AjuI GAANNNNNNNTTGG 2 cut(s) 490, 522
AluBI AGCT 3 cut(s) 176, 480, 520
AluI AGCT 3 cut(s) 176, 480, 520
Alw26I GTCTC 2 cut(s) 254, 540
AlwI GGATC 4 cut(s) 108, 259, 272, 285
AlwNI CAGNNNCTG 1 cut(s) 684
Aor13HI TCCGGA 1 cut(s) 383
AoxI GGCC 3 cut(s) 83, 311, 349
ArsI GACNNNNNNTTYG 2 cut(s) 428, 460
Asp700I GAANNNNTTC 1 cut(s) 375
Asp718I GGTACC 1 cut(s) 117
AspS9I GGNCC 1 cut(s) 386
AsuC2I CCSGG 1 cut(s) 390
AsuHPI GGTGA 3 cut(s) 311, 486, 553
AvaII GGWCC 1 cut(s) 386
BamHI GGATCC 1 cut(s) 277
BanI GGYRCC 1 cut(s) 117
BarI GAAGNNNNNNTAC 4 cut(s) 130, 162, 401, 433
BbsI GAAGAC 1 cut(s) 369
BccI CCATC 2 cut(s) 114, 575
BciT130I CCWGG 2 cut(s) 269, 282
BcnI CCSGG 1 cut(s) 390
BcoDI GTCTC 2 cut(s) 254, 540
BfmI CTRYAG 1 cut(s) 688
BmcAI AGTACT 1 cut(s) 139
Bme1390I CCNGG 3 cut(s) 269, 282, 390
Bme18I GGWCC 1 cut(s) 386
BmgT120I GGNCC 1 cut(s) 386
BmiI GGNNCC 3 cut(s) 119, 279, 388
BmrFI CCNGG 3 cut(s) 269, 282, 390
BmsI GCATC 2 cut(s) 49, 98
BpiI GAAGAC 1 cut(s) 369
BpmI CTGGAG 1 cut(s) 156
BpuMI CCSGG 1 cut(s) 390
BsaBI GATNNNNATC 1 cut(s) 105
BsaWI WCCGGW 1 cut(s) 383
BsaXI ACNNNNNCTCC 2 cut(s) 516, 546
Bsc4I CCNNNNNNNGG 2 cut(s) 274, 395
Bse1I ACTGG 3 cut(s) 145, 430, 700
Bse8I GATNNNNATC 1 cut(s) 105
BseAI TCCGGA 1 cut(s) 383
BseBI CCWGG 2 cut(s) 269, 282
BseGI GGATG 2 cut(s) 570, 621
BseJI GATNNNNATC 1 cut(s) 105
BseLI CCNNNNNNNGG 2 cut(s) 274, 395
BseNI ACTGG 3 cut(s) 145, 430, 700
BseYI CCCAGC 1 cut(s) 520
Bsh1236I CGCG 1 cut(s) 627
BshFI GGCC 3 cut(s) 85, 313, 351
BshNI GGYRCC 1 cut(s) 117
BsiSI CCGG 2 cut(s) 384, 390
BslFI GGGAC 3 cut(s) 430, 656, 688
BslI CCNNNNNNNGG 2 cut(s) 274, 395
BsmAI GTCTC 2 cut(s) 254, 540
BsmBI CGTCTC 1 cut(s) 254
BsmFI GGGAC 3 cut(s) 430, 656, 688
BsnI GGCC 3 cut(s) 85, 313, 351
Bsp13I TCCGGA 1 cut(s) 383
Bsp143I GATC 3 cut(s) 100, 264, 277
BspACI CCGC 1 cut(s) 625
BspANI GGCC 3 cut(s) 85, 313, 351
BspEI TCCGGA 1 cut(s) 383
BspFNI CGCG 1 cut(s) 627
BspHI TCATGA 1 cut(s) 52
BspLI GGNNCC 3 cut(s) 119, 279, 388
BspPI GGATC 4 cut(s) 108, 259, 272, 285
BspT107I GGYRCC 1 cut(s) 117
BsrI ACTGG 3 cut(s) 145, 430, 700
BssMI GATC 3 cut(s) 100, 264, 277
Bst2UI CCWGG 2 cut(s) 269, 282
Bst4CI ACNGT 4 cut(s) 80, 323, 346, 680
BstC8I GCNNGC 1 cut(s) 87
BstDEI CTNAG 1 cut(s) 290
BstF5I GGATG 2 cut(s) 570, 621
BstFNI CGCG 1 cut(s) 627
BstKTI GATC 3 cut(s) 103, 267, 280
BstMAI GTCTC 2 cut(s) 254, 540
BstMBI GATC 3 cut(s) 100, 264, 277
BstMWI GCNNNNNNNGC 3 cut(s) 82, 300, 477
BstNI CCWGG 2 cut(s) 269, 282
BstSCI CCNGG 3 cut(s) 267, 280, 388
BstSFI CTRYAG 1 cut(s) 688
BstUI CGCG 1 cut(s) 627
BstV2I GAAGAC 1 cut(s) 369
BstX2I RGATCY 2 cut(s) 100, 277
BstYI RGATCY 2 cut(s) 100, 277
BsuRI GGCC 3 cut(s) 85, 313, 351
BtgZI GCGATG 1 cut(s) 642
BtsCI GGATG 2 cut(s) 570, 621
BtsIMutI CAGTG 1 cut(s) 76
Cac8I GCNNGC 1 cut(s) 87
CaiI CAGNNNCTG 1 cut(s) 684
CciI TCATGA 1 cut(s) 52
Cfr13I GGNCC 1 cut(s) 386
Csp6I GTAC 3 cut(s) 118, 138, 225
CviAII CATG 4 cut(s) 53, 238, 580, 784
CviJI RGCY 9 cut(s) 85, 176, 313, 351, 393, 480, 520, 645, 693
CviKI_1 RGCY 9 cut(s) 85, 176, 313, 351, 393, 480, 520, 645, 693
CviQI GTAC 3 cut(s) 118, 138, 225
DdeI CTNAG 1 cut(s) 290
DpnI GATC 3 cut(s) 102, 266, 279
DpnII GATC 3 cut(s) 100, 264, 277
DraI TTTAAA 1 cut(s) 210
EaeI YGGCCR 1 cut(s) 83
Eco47I GGWCC 1 cut(s) 386
EcoRII CCWGG 2 cut(s) 267, 280
Esp3I CGTCTC 1 cut(s) 254
FaeI CATG 4 cut(s) 56, 241, 583, 787
FalI AAGNNNNNCTT 2 cut(s) 283, 315
FaqI GGGAC 3 cut(s) 430, 656, 688
FatI CATG 4 cut(s) 52, 237, 579, 783
FblI GTMKAC 1 cut(s) 286
FokI GGATG 2 cut(s) 557, 608
GsaI CCCAGC 1 cut(s) 524
GsuI CTGGAG 1 cut(s) 156
HaeIII GGCC 3 cut(s) 85, 313, 351
HapII CCGG 2 cut(s) 384, 390
Hin1II CATG 4 cut(s) 56, 241, 583, 787
HinfI GANTC 7 cut(s) 196, 243, 316, 443, 590, 696, 812
HpaII CCGG 2 cut(s) 384, 390
HphI GGTGA 3 cut(s) 311, 486, 553
Hpy166II GTNNAC 1 cut(s) 287
Hpy188I TCNGA 3 cut(s) 18, 165, 745
Hpy188III TCNNGA 2 cut(s) 53, 384
Hpy8I GTNNAC 1 cut(s) 287
HpyAV CCTTC 2 cut(s) 427, 585
HpyCH4III ACNGT 4 cut(s) 80, 323, 346, 680
HpyCH4V TGCA 4 cut(s) 44, 62, 413, 471
HpyF10VI GCNNNNNNNGC 3 cut(s) 82, 300, 477
HpyF3I CTNAG 1 cut(s) 290
Hsp92II CATG 4 cut(s) 56, 241, 583, 787
Kpn2I TCCGGA 1 cut(s) 383
KpnI GGTACC 1 cut(s) 121
Kzo9I GATC 3 cut(s) 100, 264, 277
LweI GCATC 2 cut(s) 49, 98
MaeIII GTNAC 3 cut(s) 492, 680, 755
MalI GATC 3 cut(s) 102, 266, 279
MboI GATC 3 cut(s) 100, 264, 277
MboII GAAGA 3 cut(s) 371, 374, 533
MfeI CAATTG 1 cut(s) 157
MflI RGATCY 2 cut(s) 100, 277
MluCI AATT 6 cut(s) 45, 157, 254, 400, 462, 771
MlyI GAGTC 1 cut(s) 452
MnlI CCTC 2 cut(s) 716, 812
MroI TCCGGA 1 cut(s) 383
MroXI GAANNNNTTC 1 cut(s) 375
MseI TTAA 2 cut(s) 209, 516
MslI CAYNNNNRTG 1 cut(s) 57
MspA1I CMGCKG 1 cut(s) 176
MspI CCGG 2 cut(s) 384, 390
MspR9I CCNGG 3 cut(s) 269, 282, 390
MunI CAATTG 1 cut(s) 157
MvaI CCWGG 2 cut(s) 269, 282
MvnI CGCG 1 cut(s) 627
MwoI GCNNNNNNNGC 3 cut(s) 82, 300, 477
NciI CCSGG 1 cut(s) 390
NdeII GATC 3 cut(s) 100, 264, 277
NlaIII CATG 4 cut(s) 56, 241, 583, 787
NlaIV GGNNCC 3 cut(s) 119, 279, 388
NmuCI GTSAC 1 cut(s) 492
PagI TCATGA 1 cut(s) 52
PdmI GAANNNNTTC 1 cut(s) 375
PfeI GAWTC 6 cut(s) 196, 243, 316, 590, 696, 812
PflMI CCANNNNNTGG 1 cut(s) 274
PleI GAGTC 1 cut(s) 451
PpsI GAGTC 1 cut(s) 451
Psp6I CCWGG 2 cut(s) 267, 280
PspFI CCCAGC 1 cut(s) 520
PspGI CCWGG 2 cut(s) 267, 280
PspN4I GGNNCC 3 cut(s) 119, 279, 388
PspPI GGNCC 1 cut(s) 386
PstNI CAGNNNCTG 1 cut(s) 684
PsuI RGATCY 2 cut(s) 100, 277
PvuII CAGCTG 1 cut(s) 176
RsaI GTAC 3 cut(s) 119, 139, 226
RsaNI GTAC 3 cut(s) 118, 138, 225
RseI CAYNNNNRTG 1 cut(s) 57
SaqAI TTAA 2 cut(s) 209, 516
Sau3AI GATC 3 cut(s) 100, 264, 277
Sau96I GGNCC 1 cut(s) 386
ScaI AGTACT 1 cut(s) 139
SchI GAGTC 1 cut(s) 452
ScrFI CCNGG 3 cut(s) 269, 282, 390
SetI ASST 9 cut(s) 119, 178, 273, 419, 482, 522, 609, 750, 792
SfaNI GCATC 2 cut(s) 49, 98
SfcI CTRYAG 1 cut(s) 688
SinI GGWCC 1 cut(s) 386
SmiMI CAYNNNNRTG 1 cut(s) 57
Sse9I AATT 6 cut(s) 45, 157, 254, 400, 462, 771
SsiI CCGC 1 cut(s) 625
StyD4I CCNGG 3 cut(s) 267, 280, 388
TaaI ACNGT 4 cut(s) 80, 323, 346, 680
TaqI TCGA 1 cut(s) 434
TasI AATT 6 cut(s) 45, 157, 254, 400, 462, 771
TatI WGTACW 1 cut(s) 137
TfiI GAWTC 6 cut(s) 196, 243, 316, 590, 696, 812
Tru1I TTAA 2 cut(s) 209, 516
Tru9I TTAA 2 cut(s) 209, 516
TscAI CASTG 1 cut(s) 83
TseFI GTSAC 1 cut(s) 492
Tsp45I GTSAC 1 cut(s) 492
TspDTI ATGAA 4 cut(s) 267, 600, 610, 800
TspRI CASTG 1 cut(s) 83
Van91I CCANNNNNTGG 1 cut(s) 274
VpaK11BI GGWCC 1 cut(s) 386
XmiI GTMKAC 1 cut(s) 286
XmnI GAANNNNTTC 1 cut(s) 375
ZrmI AGTACT 1 cut(s) 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.