RLG00000025256

synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0009298
Physical Location & Seq
Forward (+)
43417793 .. 43419179
1387 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025256

Sequence Viewer

Length: 957 bp
ATGTCAGACCAACCCGAATCCACAAAACCCACTTCCCAAAATGCCCTCCAAGCCACACAGAATTACCCTGTGCCCCTCTCCCCGCCTCTCCCACCCATCTCAAAGAACATAGAGCTGGCGAGAGCCATGTCCGCCTCTTCCAAATCCAGCCTCTTTTCTCTATCGCGGGACGACGTCGTTTACGAGGACCAGTGGCTCATTGTTGTCAACAAGCCTCAGGGGATTTACTGTGAGACTGTGCTGGAATCGGTCCCTAAGCTTCTCTTTGACTCGGCCGAGTCAGTTGCAGGGACTGAAGCTAAAGTGTCAGAGCTCCATCTTGCAAACCGTCTTGATCGTGACACTAGTGGAGTAATGATAATAACCAAGTCACATAAAGTAGCTGCCAAACTCGTTCAGGCATTTACTGATCATAAAGTTTCCAAAACGTACATTGCACGCTGCATTGGTTCAGCTCCGAAATGGGAAAGAATCACTGTTAGATCAGGTCATGGTAGGTCAAAGTTTGGGGCCTGGAGGGTGTATGCTGCTTCAGATGTAGGACGGGCACTTCCAGGTGGATCAGCAGTCAGAGATATGGAAACGTCATTTGAAATAATATCGATTAATGGACACGGGAGCTTCAAAGAGCCATCAGAATTTAGAAAAGATGAAGATAATATTGTACTAGTTGAAGAAAAATCTGTAATAGATGGAGATGCAAAGAAGGATGAGATTCTGGTGAGAGCACGTCCTCAAAGTGGAAGGACCCACCAAATCCGCTTGCATTGCCAGTATCTTGGAATTTCGATAAGAGGGGATGTTAAGTATGAGGGTGTCTATGAGTGGAAAGGGACTACATATGAAGGCCATGAACTTCATGCAGAAGGCTTGTCTTTGGAGCACCCTATTACTGGTCTTCCTGTATTATTTCGAGCACCCCTACCATCTTGGGCCAGCCAGGCGTTGCAGCCATAG

Protein Analysis

319

Amino Acids

35.09

Weight (kDa)

6.81

Isoelectric Point (pI)

42.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PseudoU_synth_2 PF00849 66 - 258 4.6e-23 RNA pseudouridylate synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 177
AccII CGCG 1 cut(s) 166
AciI CCGC 4 cut(s) 83, 132, 166, 760
AclWI GGATC 1 cut(s) 568
AcoI YGGCCR 1 cut(s) 273
AcsI RAATTY 2 cut(s) 638, 783
AcuI CTGAAG 2 cut(s) 315, 516
AcyI GRCGYC 1 cut(s) 174
AfaI GTAC 2 cut(s) 431, 666
AfiI CCNNNNNNNGG 2 cut(s) 740, 893
AgsI TTSAA 3 cut(s) 593, 625, 674
AhlI ACTAGT 2 cut(s) 344, 667
AjiI CACGTC 1 cut(s) 731
AjnI CCWGG 3 cut(s) 512, 553, 939
AluBI AGCT 7 cut(s) 115, 259, 299, 313, 383, 455, 621
AluI AGCT 7 cut(s) 115, 259, 299, 313, 383, 455, 621
Alw21I GWGCWC 4 cut(s) 315, 730, 885, 919
Alw26I GTCTC 1 cut(s) 227
AlwI GGATC 1 cut(s) 568
AlwNI CAGNNNCTG 1 cut(s) 293
AoxI GGCC 4 cut(s) 273, 510, 847, 933
ApeKI GCWGC 4 cut(s) 383, 441, 527, 949
ApoI RAATTY 2 cut(s) 638, 783
AseI ATTAAT 1 cut(s) 606
AspS9I GGNCC 5 cut(s) 187, 250, 510, 747, 933
AsuHPI GGTGA 1 cut(s) 733
AvaII GGWCC 3 cut(s) 187, 250, 747
AxyI CCTNAGG 1 cut(s) 216
BaeGI GKGCMC 2 cut(s) 75, 550
BanII GRGCYC 1 cut(s) 315
BbsI GAAGAC 1 cut(s) 890
Bbv12I GWGCWC 4 cut(s) 315, 730, 885, 919
BbvI GCAGC 3 cut(s) 370, 428, 514
BccI CCATC 5 cut(s) 104, 324, 640, 686, 934
BcgI CGANNNNNNTGC 2 cut(s) 266, 300
BciT130I CCWGG 3 cut(s) 514, 555, 941
BclI TGATCA 1 cut(s) 409
BcoDI GTCTC 1 cut(s) 227
BcuI ACTAGT 2 cut(s) 344, 667
BfaI CTAG 2 cut(s) 345, 668
BglI GCCNNNNNGGC 1 cut(s) 941
BisI GCNGC 4 cut(s) 384, 442, 528, 950
BlsI GCNGC 4 cut(s) 385, 443, 529, 951
Bme1390I CCNGG 3 cut(s) 514, 555, 941
Bme18I GGWCC 3 cut(s) 187, 250, 747
BmgBI CACGTC 1 cut(s) 731
BmgT120I GGNCC 5 cut(s) 187, 250, 510, 747, 933
BmiI GGNNCC 3 cut(s) 252, 511, 749
BmrFI CCNGG 3 cut(s) 514, 555, 941
BmsI GCATC 1 cut(s) 688
BpiI GAAGAC 1 cut(s) 890
BpmI CTGGAG 1 cut(s) 535
Bpu10I CCTNAGC 1 cut(s) 255
Bsa29I ATCGAT 1 cut(s) 602
BsaHI GRCGYC 1 cut(s) 174
Bsc4I CCNNNNNNNGG 2 cut(s) 740, 893
Bse1I ACTGG 3 cut(s) 190, 772, 898
Bse21I CCTNAGG 1 cut(s) 216
Bse3DI GCAATG 2 cut(s) 432, 766
BseBI CCWGG 3 cut(s) 514, 555, 941
BseCI ATCGAT 1 cut(s) 602
BseGI GGATG 2 cut(s) 715, 805
BseLI CCNNNNNNNGG 2 cut(s) 740, 893
BseMI GCAATG 2 cut(s) 432, 766
BseMII CTCAG 1 cut(s) 230
BseNI ACTGG 3 cut(s) 190, 772, 898
BseSI GKGCMC 2 cut(s) 75, 550
BseX3I CGGCCG 1 cut(s) 273
BseXI GCAGC 3 cut(s) 370, 428, 514
Bsh1236I CGCG 1 cut(s) 166
Bsh1285I CGRYCG 1 cut(s) 276
BshFI GGCC 4 cut(s) 275, 512, 849, 935
BshVI ATCGAT 1 cut(s) 602
BsiEI CGRYCG 1 cut(s) 276
BsiHKAI GWGCWC 4 cut(s) 315, 730, 885, 919
BslFI GGGAC 4 cut(s) 182, 236, 304, 847
BslI CCNNNNNNNGG 2 cut(s) 740, 893
BsmAI GTCTC 1 cut(s) 227
BsmFI GGGAC 4 cut(s) 182, 236, 304, 847
BsnI GGCC 4 cut(s) 275, 512, 849, 935
Bsp1286I GDGCHC 6 cut(s) 75, 315, 550, 730, 885, 919
Bsp143I GATC 4 cut(s) 334, 409, 482, 560
BspACI CCGC 4 cut(s) 83, 132, 166, 760
BspANI GGCC 4 cut(s) 275, 512, 849, 935
BspCNI CTCAG 1 cut(s) 229
BspDI ATCGAT 1 cut(s) 602
BspFNI CGCG 1 cut(s) 166
BspLI GGNNCC 3 cut(s) 252, 511, 749
BspPI GGATC 1 cut(s) 568
BsrDI GCAATG 2 cut(s) 432, 766
BsrI ACTGG 3 cut(s) 190, 772, 898
BssMI GATC 4 cut(s) 334, 409, 482, 560
BssNI GRCGYC 1 cut(s) 174
Bst2UI CCWGG 3 cut(s) 514, 555, 941
Bst4CI ACNGT 4 cut(s) 230, 238, 329, 478
Bst6I CTCTTC 1 cut(s) 142
BstACI GRCGYC 1 cut(s) 174
BstC8I GCNNGC 4 cut(s) 117, 439, 764, 937
BstDEI CTNAG 2 cut(s) 216, 255
BstF5I GGATG 2 cut(s) 715, 805
BstFNI CGCG 1 cut(s) 166
BstKTI GATC 4 cut(s) 337, 412, 485, 563
BstMAI GTCTC 1 cut(s) 227
BstMBI GATC 4 cut(s) 334, 409, 482, 560
BstMCI CGRYCG 1 cut(s) 276
BstMWI GCNNNNNNNGC 4 cut(s) 50, 131, 768, 941
BstNI CCWGG 3 cut(s) 514, 555, 941
BstSCI CCNGG 3 cut(s) 512, 553, 939
BstSLI GKGCMC 2 cut(s) 75, 550
BstUI CGCG 1 cut(s) 166
BstV1I GCAGC 3 cut(s) 370, 428, 514
BstV2I GAAGAC 1 cut(s) 890
BstXI CCANNNNNNTGG 1 cut(s) 779
BstZI CGGCCG 1 cut(s) 273
Bsu15I ATCGAT 1 cut(s) 602
Bsu36I CCTNAGG 1 cut(s) 216
BsuRI GGCC 4 cut(s) 275, 512, 849, 935
BsuTUI ATCGAT 1 cut(s) 602
BtrI CACGTC 1 cut(s) 731
BtsCI GGATG 2 cut(s) 715, 805
BtsIMutI CAGTG 2 cut(s) 197, 474
Cac8I GCNNGC 4 cut(s) 117, 439, 764, 937
CaiI CAGNNNCTG 1 cut(s) 293
Cfr13I GGNCC 5 cut(s) 187, 250, 510, 747, 933
ClaI ATCGAT 1 cut(s) 602
Csp6I GTAC 2 cut(s) 430, 665
CviAII CATG 4 cut(s) 127, 491, 851, 860
CviQI GTAC 2 cut(s) 430, 665
DdeI CTNAG 2 cut(s) 216, 255
DpnI GATC 4 cut(s) 336, 411, 484, 562
DpnII GATC 4 cut(s) 334, 409, 482, 560
EaeI YGGCCR 1 cut(s) 273
EagI CGGCCG 1 cut(s) 273
Eam1104I CTCTTC 1 cut(s) 142
EarI CTCTTC 1 cut(s) 142
EciI GGCGGA 1 cut(s) 121
Ecl136II GAGCTC 1 cut(s) 313
EclXI CGGCCG 1 cut(s) 273
Eco24I GRGCYC 1 cut(s) 315
Eco47I GGWCC 3 cut(s) 187, 250, 747
Eco52I CGGCCG 1 cut(s) 273
Eco53kI GAGCTC 1 cut(s) 313
Eco57I CTGAAG 2 cut(s) 315, 516
Eco81I CCTNAGG 1 cut(s) 216
EcoICRI GAGCTC 1 cut(s) 313
EcoO109I RGGNCCY 2 cut(s) 510, 747
EcoRII CCWGG 3 cut(s) 512, 553, 939
EcoT38I GRGCYC 1 cut(s) 315
FaeI CATG 4 cut(s) 130, 494, 854, 863
FalI AAGNNNNNCTT 2 cut(s) 248, 280
FaqI GGGAC 4 cut(s) 182, 236, 304, 847
FatI CATG 4 cut(s) 126, 490, 850, 859
FauI CCCGC 2 cut(s) 90, 159
FauNDI CATATG 1 cut(s) 841
FbaI TGATCA 1 cut(s) 409
Fnu4HI GCNGC 4 cut(s) 384, 442, 528, 950
FokI GGATG 2 cut(s) 722, 812
FriOI GRGCYC 1 cut(s) 315
Fsp4HI GCNGC 4 cut(s) 384, 442, 528, 950
FspBI CTAG 2 cut(s) 345, 668
GluI GCNGC 4 cut(s) 384, 442, 528, 950
GsuI CTGGAG 1 cut(s) 535
HaeIII GGCC 4 cut(s) 275, 512, 849, 935
Hin1I GRCGYC 1 cut(s) 174
Hin1II CATG 4 cut(s) 130, 494, 854, 863
HincII GTYRAC 1 cut(s) 208
HindII GTYRAC 1 cut(s) 208
HindIII AAGCTT 1 cut(s) 257
HinfI GANTC 6 cut(s) 17, 245, 269, 278, 471, 715
HphI GGTGA 1 cut(s) 733
Hpy166II GTNNAC 2 cut(s) 181, 208
Hpy188I TCNGA 6 cut(s) 7, 310, 459, 535, 572, 637
Hpy188III TCNNGA 2 cut(s) 332, 338
Hpy8I GTNNAC 2 cut(s) 181, 208
Hpy99I CGWCG 2 cut(s) 176, 179
HpyAV CCTTC 4 cut(s) 700, 738, 839, 860
HpyCH4III ACNGT 4 cut(s) 230, 238, 329, 478
HpyCH4IV ACGT 4 cut(s) 174, 428, 584, 730
HpyCH4V TGCA 8 cut(s) 287, 323, 437, 444, 701, 766, 863, 949
HpyF10VI GCNNNNNNNGC 4 cut(s) 50, 131, 768, 941
HpyF3I CTNAG 2 cut(s) 216, 255
HpySE526I ACGT 4 cut(s) 174, 428, 584, 730
Hsp92I GRCGYC 1 cut(s) 174
Hsp92II CATG 4 cut(s) 130, 494, 854, 863
Ksp22I TGATCA 1 cut(s) 409
Kzo9I GATC 4 cut(s) 334, 409, 482, 560
LmnI GCTCC 4 cut(s) 318, 460, 618, 880
Lsp1109I GCAGC 3 cut(s) 370, 428, 514
LweI GCATC 1 cut(s) 688
MaeI CTAG 2 cut(s) 345, 668
MaeII ACGT 4 cut(s) 174, 428, 584, 730
MaeIII GTNAC 2 cut(s) 338, 369
MalI GATC 4 cut(s) 336, 411, 484, 562
MboI GATC 4 cut(s) 334, 409, 482, 560
MboII GAAGA 4 cut(s) 129, 665, 686, 890
MhlI GDGCHC 6 cut(s) 75, 315, 550, 730, 885, 919
MluCI AATT 3 cut(s) 61, 638, 783
MlyI GAGTC 2 cut(s) 263, 287
MseI TTAA 2 cut(s) 606, 804
MspR9I CCNGG 3 cut(s) 514, 555, 941
MvaI CCWGG 3 cut(s) 514, 555, 941
MvnI CGCG 1 cut(s) 166
MwoI GCNNNNNNNGC 4 cut(s) 50, 131, 768, 941
NdeI CATATG 1 cut(s) 841
NdeII GATC 4 cut(s) 334, 409, 482, 560
NlaIII CATG 4 cut(s) 130, 494, 854, 863
NlaIV GGNNCC 3 cut(s) 252, 511, 749
NmeAIII GCCGAG 2 cut(s) 251, 301
NmuCI GTSAC 2 cut(s) 338, 369
PcsI WCGNNNNNNNCGW 1 cut(s) 180
PfeI GAWTC 4 cut(s) 17, 245, 471, 715
PflFI GACNNNGTC 1 cut(s) 173
PkrI GCNGC 4 cut(s) 385, 443, 529, 951
PleI GAGTC 2 cut(s) 263, 286
PpsI GAGTC 2 cut(s) 263, 286
PpuMI RGGWCCY 1 cut(s) 747
PshBI ATTAAT 1 cut(s) 606
Psp124BI GAGCTC 1 cut(s) 315
Psp5II RGGWCCY 1 cut(s) 747
Psp6I CCWGG 3 cut(s) 512, 553, 939
PspGI CCWGG 3 cut(s) 512, 553, 939
PspN4I GGNNCC 3 cut(s) 252, 511, 749
PspPI GGNCC 5 cut(s) 187, 250, 510, 747, 933
PspPPI RGGWCCY 1 cut(s) 747
PstNI CAGNNNCTG 1 cut(s) 293
PsyI GACNNNGTC 1 cut(s) 173
RsaI GTAC 2 cut(s) 431, 666
RsaNI GTAC 2 cut(s) 430, 665
SacI GAGCTC 1 cut(s) 315
SaqAI TTAA 2 cut(s) 606, 804
SatI GCNGC 4 cut(s) 384, 442, 528, 950
Sau3AI GATC 4 cut(s) 334, 409, 482, 560
Sau96I GGNCC 5 cut(s) 187, 250, 510, 747, 933
SchI GAGTC 2 cut(s) 263, 287
ScrFI CCNGG 3 cut(s) 514, 555, 941
SduI GDGCHC 6 cut(s) 75, 315, 550, 730, 885, 919
SfaNI GCATC 1 cut(s) 688
SinI GGWCC 3 cut(s) 187, 250, 747
SpeI ACTAGT 2 cut(s) 344, 667
Sse9I AATT 3 cut(s) 61, 638, 783
SsiI CCGC 4 cut(s) 83, 132, 166, 760
SspI AATATT 1 cut(s) 661
SspMI CTAG 2 cut(s) 345, 668
SstI GAGCTC 1 cut(s) 315
StyD4I CCNGG 3 cut(s) 512, 553, 939
TaaI ACNGT 4 cut(s) 230, 238, 329, 478
TaiI ACGT 4 cut(s) 177, 431, 587, 733
TaqI TCGA 3 cut(s) 602, 788, 913
TaqII GACCGA 1 cut(s) 238
TasI AATT 3 cut(s) 61, 638, 783
TatI WGTACW 1 cut(s) 664
TfiI GAWTC 4 cut(s) 17, 245, 471, 715
Tru1I TTAA 2 cut(s) 606, 804
Tru9I TTAA 2 cut(s) 606, 804
TscAI CASTG 2 cut(s) 197, 481
TseFI GTSAC 2 cut(s) 338, 369
TseI GCWGC 4 cut(s) 383, 441, 527, 949
Tsp45I GTSAC 2 cut(s) 338, 369
TspDTI ATGAA 4 cut(s) 666, 848, 858, 867
TspRI CASTG 2 cut(s) 197, 481
Tth111I GACNNNGTC 1 cut(s) 173
VpaK11BI GGWCC 3 cut(s) 187, 250, 747
VspI ATTAAT 1 cut(s) 606
XapI RAATTY 2 cut(s) 638, 783
XspI CTAG 2 cut(s) 345, 668
ZraI GACGTC 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.