pycom12g21430

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0011571
Physical Location & Seq
Forward (+)
22601459 .. 22602582
1124 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 891 bp
ATGGATCGGATGAGGCCGGTGTACGTCAGGCAGAAGAGCAATAGTAGTGGCGGCACCCCGGGGCCACCAACTTCGCCAATGATGTCGCCAATGAACCACCATGGGAGGTCGGGGTCGGTTGGAATGGTGGGTGCCAGGAAAGTGCAGCACACGAAAGCCGCAGCGCAAAGGCTTGCACATGTGATGGCTCACAAACCAGCTGACGACGATGATGAGGACGACGACGATCTTTCTTATGATCCTGGCTTGTCAAGCGGCACAGGCAGCCTTGGACTTGTCGGCGGAAGATCAATGCGCCCACGCTCTCCAATGTCAGTTCGTGCCGCTCAAGAACATTCTACAACTACACGGGGACGATCATCACCGTCTCTTAACTCCATGGAACAACCATCTTCTACCCATTCGATACCATCTAGTCGACCATATCAGTACGCAGAACAACCGTCATCAGCTCATTCAACACCAGCAAGCCGAACATACCAATCCATCAACACAAAACCCATTCACCCTGTTGTCGAACAACCACATTCTCTTCGCTCTTTAATCGCTACTAGACCATCCCAGCCTTCAAATTCCAACGAACAACCTACCTCTGCTCGTACTATATCAGGCATGAACTCTGCTCGATCATCTCAGCCAACAAATGTTGTCGACCAACCTACCTCTGCTCGTTCTATATCAGGCCTGAACTCTGCCCGGTCATCTCAGCCTACCCCTGCTGTCGAACAACCTACCTCTGCACGCTCTTTAGCTGCTGCCCGTCCGCAGTTAGGAACCAAGCCAGTCCACATCATGCCAGCTAGTGTACCTATATCACTTAGGCCACCCTCTTCTGCTGATCCTCCAGCTGATAATCGAAGAGAGCCAGCACCAGTACCACTACGCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

297

Amino Acids

31.39

Weight (kDa)

10.63

Isoelectric Point (pI)

76.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 53, 131
AccBSI CCGCTC 1 cut(s) 326
AccI GTMKAC 2 cut(s) 418, 651
AciI CCGC 6 cut(s) 51, 159, 255, 282, 324, 764
AclWI GGATC 3 cut(s) 12, 233, 833
AcsI RAATTY 1 cut(s) 571
AfaI GTAC 5 cut(s) 23, 431, 601, 807, 876
AfiI CCNNNNNNNGG 1 cut(s) 770
AflIII ACRYGT 1 cut(s) 178
AgsI TTSAA 2 cut(s) 459, 570
AjnI CCWGG 2 cut(s) 134, 241
AluBI AGCT 5 cut(s) 200, 452, 752, 800, 848
AluI AGCT 5 cut(s) 200, 452, 752, 800, 848
Alw26I GTCTC 1 cut(s) 372
AlwI GGATC 3 cut(s) 12, 233, 833
Ama87I CYCGRG 1 cut(s) 58
AoxI GGCC 4 cut(s) 14, 62, 682, 821
ApeKI GCWGC 5 cut(s) 145, 161, 264, 752, 755
ApoI RAATTY 1 cut(s) 571
AspLEI GCGC 2 cut(s) 166, 297
AspS9I GGNCC 1 cut(s) 62
AsuC2I CCSGG 3 cut(s) 59, 60, 697
AsuHPI GGTGA 2 cut(s) 354, 497
AvaI CYCGRG 1 cut(s) 58
BanI GGYRCC 2 cut(s) 53, 131
BbvI GCAGC 5 cut(s) 157, 173, 276, 739, 742
BccI CCATC 5 cut(s) 178, 397, 418, 494, 565
BciT130I CCWGG 2 cut(s) 136, 243
BcnI CCSGG 3 cut(s) 59, 60, 697
BcoDI GTCTC 1 cut(s) 372
BfaI CTAG 3 cut(s) 414, 552, 801
BisI GCNGC 9 cut(s) 52, 146, 159, 162, 256, 265, 324, 753, 756
BlsI GCNGC 9 cut(s) 53, 147, 160, 163, 257, 266, 325, 754, 757
Bme1390I CCNGG 5 cut(s) 59, 60, 136, 243, 697
BmeT110I CYCGRG 1 cut(s) 58
BmgT120I GGNCC 1 cut(s) 62
BmiI GGNNCC 4 cut(s) 55, 63, 133, 775
BmrFI CCNGG 5 cut(s) 59, 60, 136, 243, 697
BpmI CTGGAG 1 cut(s) 828
BpuEI CTTGAG 1 cut(s) 312
BpuMI CCSGG 3 cut(s) 59, 60, 697
BsaJI CCNNGG 6 cut(s) 57, 58, 59, 100, 268, 378
Bsc4I CCNNNNNNNGG 1 cut(s) 770
Bse118I RCCGGY 1 cut(s) 16
Bse1I ACTGG 2 cut(s) 782, 872
BseBI CCWGG 2 cut(s) 136, 243
BseDI CCNNGG 6 cut(s) 57, 58, 59, 100, 268, 378
BseGI GGATG 2 cut(s) 15, 557
BseLI CCNNNNNNNGG 1 cut(s) 770
BseMII CTCAG 2 cut(s) 647, 719
BseNI ACTGG 2 cut(s) 782, 872
BseXI GCAGC 5 cut(s) 157, 173, 276, 739, 742
BseYI CCCAGC 1 cut(s) 561
BsgI GTGCAG 2 cut(s) 164, 723
BshFI GGCC 4 cut(s) 16, 64, 684, 823
BshNI GGYRCC 2 cut(s) 53, 131
BsiHKCI CYCGRG 1 cut(s) 58
BsiSI CCGG 3 cut(s) 17, 59, 697
BslFI GGGAC 1 cut(s) 366
BslI CCNNNNNNNGG 1 cut(s) 770
BsmAI GTCTC 1 cut(s) 372
BsmBI CGTCTC 1 cut(s) 372
BsmFI GGGAC 1 cut(s) 366
BsnI GGCC 4 cut(s) 16, 64, 684, 823
BsoBI CYCGRG 1 cut(s) 58
Bsp143I GATC 7 cut(s) 4, 226, 238, 287, 356, 626, 838
Bsp19I CCATGG 2 cut(s) 100, 378
BspACI CCGC 6 cut(s) 51, 159, 255, 282, 324, 764
BspANI GGCC 4 cut(s) 16, 64, 684, 823
BspCNI CTCAG 2 cut(s) 646, 718
BspLI GGNNCC 4 cut(s) 55, 63, 133, 775
BspPI GGATC 3 cut(s) 12, 233, 833
BspQI GCTCTTC 1 cut(s) 29
BspT107I GGYRCC 2 cut(s) 53, 131
BsrBI CCGCTC 1 cut(s) 326
BsrFI RCCGGY 1 cut(s) 16
BsrI ACTGG 2 cut(s) 782, 872
BssAI RCCGGY 1 cut(s) 16
BssECI CCNNGG 6 cut(s) 57, 58, 59, 100, 268, 378
BssMI GATC 7 cut(s) 4, 226, 238, 287, 356, 626, 838
BssT1I CCWWGG 3 cut(s) 100, 268, 378
Bst2UI CCWGG 2 cut(s) 136, 243
Bst4CI ACNGT 2 cut(s) 366, 444
Bst6I CTCTTC 4 cut(s) 29, 537, 835, 853
BstC8I GCNNGC 5 cut(s) 174, 469, 742, 798, 867
BstDEI CTNAG 3 cut(s) 633, 705, 818
BstDSI CCRYGG 2 cut(s) 100, 378
BstF5I GGATG 2 cut(s) 15, 557
BstHHI GCGC 2 cut(s) 166, 297
BstKTI GATC 7 cut(s) 7, 229, 241, 290, 359, 629, 841
BstMAI GTCTC 1 cut(s) 372
BstMBI GATC 7 cut(s) 4, 226, 238, 287, 356, 626, 838
BstMWI GCNNNNNNNGC 3 cut(s) 252, 261, 264
BstNI CCWGG 2 cut(s) 136, 243
BstNSI RCATGY 1 cut(s) 182
BstSCI CCNGG 5 cut(s) 57, 58, 134, 241, 695
BstV1I GCAGC 5 cut(s) 157, 173, 276, 739, 742
BsuRI GGCC 4 cut(s) 16, 64, 684, 823
BtgI CCRYGG 2 cut(s) 100, 378
BtsCI GGATG 2 cut(s) 15, 557
Cac8I GCNNGC 5 cut(s) 174, 469, 742, 798, 867
CfoI GCGC 2 cut(s) 166, 297
Cfr10I RCCGGY 1 cut(s) 16
Cfr13I GGNCC 1 cut(s) 62
Cfr9I CCCGGG 1 cut(s) 58
Csp6I GTAC 5 cut(s) 22, 430, 600, 806, 875
CspCI CAANNNNNGTGG 2 cut(s) 28, 63
CviAII CATG 5 cut(s) 101, 179, 379, 613, 793
CviQI GTAC 5 cut(s) 22, 430, 600, 806, 875
DdeI CTNAG 3 cut(s) 633, 705, 818
DpnI GATC 7 cut(s) 6, 228, 240, 289, 358, 628, 840
DpnII GATC 7 cut(s) 4, 226, 238, 287, 356, 626, 838
Eam1104I CTCTTC 4 cut(s) 29, 537, 835, 853
EarI CTCTTC 4 cut(s) 29, 537, 835, 853
EciI GGCGGA 1 cut(s) 297
Eco130I CCWWGG 3 cut(s) 100, 268, 378
Eco147I AGGCCT 1 cut(s) 684
Eco88I CYCGRG 1 cut(s) 58
EcoRII CCWGG 2 cut(s) 134, 241
EcoT14I CCWWGG 3 cut(s) 100, 268, 378
ErhI CCWWGG 3 cut(s) 100, 268, 378
Esp3I CGTCTC 1 cut(s) 372
FaeI CATG 5 cut(s) 104, 182, 382, 616, 796
FaqI GGGAC 1 cut(s) 366
FatI CATG 5 cut(s) 100, 178, 378, 612, 792
FblI GTMKAC 2 cut(s) 418, 651
Fnu4HI GCNGC 9 cut(s) 52, 146, 159, 162, 256, 265, 324, 753, 756
FokI GGATG 2 cut(s) 22, 544
Fsp4HI GCNGC 9 cut(s) 52, 146, 159, 162, 256, 265, 324, 753, 756
FspBI CTAG 3 cut(s) 414, 552, 801
GlaI GCGC 2 cut(s) 165, 296
GluI GCNGC 9 cut(s) 52, 146, 159, 162, 256, 265, 324, 753, 756
GsaI CCCAGC 1 cut(s) 565
GsuI CTGGAG 1 cut(s) 828
HaeIII GGCC 4 cut(s) 16, 64, 684, 823
HapII CCGG 3 cut(s) 17, 59, 697
HhaI GCGC 2 cut(s) 166, 297
Hin1II CATG 5 cut(s) 104, 182, 382, 616, 796
Hin6I GCGC 2 cut(s) 164, 295
HinP1I GCGC 2 cut(s) 164, 295
HincII GTYRAC 2 cut(s) 419, 652
HindII GTYRAC 2 cut(s) 419, 652
HpaII CCGG 3 cut(s) 17, 59, 697
HphI GGTGA 2 cut(s) 354, 497
Hpy166II GTNNAC 5 cut(s) 22, 419, 652, 787, 806
Hpy188I TCNGA 1 cut(s) 9
Hpy188III TCNNGA 1 cut(s) 329
Hpy8I GTNNAC 5 cut(s) 22, 419, 652, 787, 806
Hpy99I CGWCG 3 cut(s) 209, 224, 227
HpyAV CCTTC 1 cut(s) 576
HpyCH4III ACNGT 2 cut(s) 366, 444
HpyCH4IV ACGT 1 cut(s) 24
HpyCH4V TGCA 3 cut(s) 145, 176, 740
HpyF10VI GCNNNNNNNGC 3 cut(s) 252, 261, 264
HpyF3I CTNAG 3 cut(s) 633, 705, 818
HpySE526I ACGT 1 cut(s) 24
Hsp92II CATG 5 cut(s) 104, 182, 382, 616, 796
HspAI GCGC 2 cut(s) 164, 295
Kzo9I GATC 7 cut(s) 4, 226, 238, 287, 356, 626, 838
LguI GCTCTTC 1 cut(s) 29
Lsp1109I GCAGC 5 cut(s) 157, 173, 276, 739, 742
MaeI CTAG 3 cut(s) 414, 552, 801
MaeII ACGT 1 cut(s) 24
MalI GATC 7 cut(s) 6, 228, 240, 289, 358, 628, 840
MbiI CCGCTC 1 cut(s) 326
MboI GATC 7 cut(s) 4, 226, 238, 287, 356, 626, 838
MboII GAAGA 6 cut(s) 46, 297, 384, 524, 822, 870
MluCI AATT 1 cut(s) 571
MmeI TCCRAC 2 cut(s) 100, 600
MnlI CCTC 8 cut(s) 6, 99, 208, 601, 673, 745, 838, 852
MseI TTAA 3 cut(s) 372, 542, 889
MspA1I CMGCKG 2 cut(s) 200, 848
MspI CCGG 3 cut(s) 17, 59, 697
MspR9I CCNGG 5 cut(s) 59, 60, 136, 243, 697
MvaI CCWGG 2 cut(s) 136, 243
MwoI GCNNNNNNNGC 3 cut(s) 252, 261, 264
NciI CCSGG 3 cut(s) 59, 60, 697
NcoI CCATGG 2 cut(s) 100, 378
NdeII GATC 7 cut(s) 4, 226, 238, 287, 356, 626, 838
NlaIII CATG 5 cut(s) 104, 182, 382, 616, 796
NlaIV GGNNCC 4 cut(s) 55, 63, 133, 775
NspI RCATGY 1 cut(s) 182
PceI AGGCCT 1 cut(s) 684
PciI ACATGT 1 cut(s) 178
PciSI GCTCTTC 1 cut(s) 29
PkrI GCNGC 9 cut(s) 53, 147, 160, 163, 257, 266, 325, 754, 757
PscI ACATGT 1 cut(s) 178
Psp6I CCWGG 2 cut(s) 134, 241
PspFI CCCAGC 1 cut(s) 561
PspGI CCWGG 2 cut(s) 134, 241
PspN4I GGNNCC 4 cut(s) 55, 63, 133, 775
PspPI GGNCC 1 cut(s) 62
PvuII CAGCTG 2 cut(s) 200, 848
RsaI GTAC 5 cut(s) 23, 431, 601, 807, 876
RsaNI GTAC 5 cut(s) 22, 430, 600, 806, 875
SalI GTCGAC 2 cut(s) 417, 650
SapI GCTCTTC 1 cut(s) 29
SaqAI TTAA 3 cut(s) 372, 542, 889
SatI GCNGC 9 cut(s) 52, 146, 159, 162, 256, 265, 324, 753, 756
Sau3AI GATC 7 cut(s) 4, 226, 238, 287, 356, 626, 838
Sau96I GGNCC 1 cut(s) 62
ScrFI CCNGG 5 cut(s) 59, 60, 136, 243, 697
SmaI CCCGGG 1 cut(s) 60
SmlI CTYRAG 1 cut(s) 327
SmoI CTYRAG 1 cut(s) 327
Sse9I AATT 1 cut(s) 571
SseBI AGGCCT 1 cut(s) 684
SsiI CCGC 6 cut(s) 51, 159, 255, 282, 324, 764
SspMI CTAG 3 cut(s) 414, 552, 801
StuI AGGCCT 1 cut(s) 684
StyD4I CCNGG 5 cut(s) 57, 58, 134, 241, 695
StyI CCWWGG 3 cut(s) 100, 268, 378
TaaI ACNGT 2 cut(s) 366, 444
TaiI ACGT 1 cut(s) 27
TaqI TCGA 7 cut(s) 404, 418, 516, 625, 651, 723, 856
TasI AATT 1 cut(s) 571
TauI GCSGC 4 cut(s) 54, 161, 258, 326
Tru1I TTAA 3 cut(s) 372, 542, 889
Tru9I TTAA 3 cut(s) 372, 542, 889
TseI GCWGC 5 cut(s) 145, 161, 264, 752, 755
TspDTI ATGAA 2 cut(s) 107, 629
TspMI CCCGGG 1 cut(s) 58
XapI RAATTY 1 cut(s) 571
XceI RCATGY 1 cut(s) 182
XmaI CCCGGG 1 cut(s) 58
XmiI GTMKAC 2 cut(s) 418, 651
XspI CTAG 3 cut(s) 414, 552, 801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.