pycom12g21660

SEC-C motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0011179
Physical Location & Seq
Forward (+)
22786793 .. 22789184
2392 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 684 bp
ATGCATCGCTCTTCGCGAATCATAAGCAGCAAATTCTGCAACAAAAACTTAAACTTCCCGTCGTCTTTCTTCGGCTACCGTTACGGTGACCGCAACCTCGCTCCGTCGTTCCACCGCCCGATCTCCTCCACGACGCACCTAGATAACTCATGGATGAACAAGATTAAAGGGGTCTTCACCGGCAACAAGACCTCTCCGGAAGATGCAAAGTCCAGCTCCGATTCGTTCACGCTGCTTCGATTTGCGGATGAGATGAAGAATGCTAGAAGAATCGGGGCGTTCAAGCAATACATTGTGGGGAGAAGCAGTGAAGCTACTTTTGCCGATGCTTTCGAGAAGCAGGAAGCGATTATTCGCTATCTCGGAGGATTTGATTCCACTGGAGAGAATCTCCAAGTTGCTCAAAAACAAGAAGCAGCAAAGCATTGTAAATGCACGATTGCTGATGTTGAGAACGCTCTTGCAAAATTCACGTGGGCTAGAGAAGCACAAAAGAAGATGGAGAATTTAAAGAAAGAAGGGAAACCAATGCCAAAGAACATTAACGAGGTGCAAAAATTGATGGGTTCAACGCCATTTGACCTTGCCAAGTCTAATTTGGCTAAGAGTGGGCAAATCAGTAGGAATGCGCTGTGTCCATGTGGCTCCAAGAAGAGATACAAACGGTGTTGCGGCAAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SEC-C

Protein Analysis

228

Amino Acids

25.5

Weight (kDa)

9.74

Isoelectric Point (pI)

49.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SEC-C PF02810 208 - 226 1.2e-06 SEC-C motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011179)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04950
fragaria_vesca FvH4_6g03930
malus_domestica MD12G1234200.v1.1
prunus_persica Prupe.6G337300_v2.0.a1
pyrus_communis pycom04g19330 pycom12g21660
rosa_chinensis RchiOBHm_Chr3g0452481 RchiOBHm_Chr3g0452521
rosa_laevigata RLG00000025595 RLG00000025598
rosa_multiflora Rmu_co8297771.1_g000001 Rmu_sc0011680.1_g000004
rosa_roxburghii Rroxscaffold_6G00429510
rosa_rugosa Rorug02G0641200
rosa_samantha Rh3AG043000 Rh3BG044700 Rh3CG043300 Rh3DG044100
rosa_wichuraiana Rw3G003330 Rw3G003360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 16
AccIII TCCGGA 1 cut(s) 196
AciI CCGC 4 cut(s) 91, 115, 245, 672
AcsI RAATTY 3 cut(s) 32, 467, 505
AcvI CACGTG 1 cut(s) 474
AgsI TTSAA 2 cut(s) 283, 570
AluBI AGCT 2 cut(s) 216, 314
AluI AGCT 2 cut(s) 216, 314
Aor13HI TCCGGA 1 cut(s) 196
ApeKI GCWGC 3 cut(s) 27, 232, 416
ApoI RAATTY 3 cut(s) 32, 467, 505
AspLEI GCGC 1 cut(s) 631
AsuHPI GGTGA 2 cut(s) 98, 169
BbrPI CACGTG 1 cut(s) 474
BbsI GAAGAC 1 cut(s) 166
BbvI GCAGC 3 cut(s) 39, 219, 428
BccI CCATC 2 cut(s) 493, 556
BfaI CTAG 3 cut(s) 140, 264, 480
BisI GCNGC 4 cut(s) 28, 233, 417, 673
BlsI GCNGC 4 cut(s) 29, 234, 418, 674
BmiI GGNNCC 1 cut(s) 646
BmsI GCATC 3 cut(s) 13, 193, 316
BpiI GAAGAC 1 cut(s) 166
BplI GAGNNNNNCTC 2 cut(s) 375, 407
BpmI CTGGAG 1 cut(s) 402
BsaAI YACGTR 1 cut(s) 474
BsaWI WCCGGW 1 cut(s) 196
BsaXI ACNNNNNCTCC 2 cut(s) 292, 322
Bse118I RCCGGY 1 cut(s) 179
Bse1I ACTGG 1 cut(s) 385
BseAI TCCGGA 1 cut(s) 196
BseGI GGATG 2 cut(s) 159, 253
BseNI ACTGG 1 cut(s) 385
BseRI GAGGAG 1 cut(s) 115
BseXI GCAGC 3 cut(s) 39, 219, 428
Bsh1236I CGCG 1 cut(s) 16
BsiSI CCGG 2 cut(s) 180, 197
BsmI GAATGC 2 cut(s) 265, 631
Bsp13I TCCGGA 1 cut(s) 196
Bsp143I GATC 1 cut(s) 120
Bsp68I TCGCGA 1 cut(s) 16
BspACI CCGC 4 cut(s) 91, 115, 245, 672
BspEI TCCGGA 1 cut(s) 196
BspFNI CGCG 1 cut(s) 16
BspLI GGNNCC 1 cut(s) 646
BspQI GCTCTTC 1 cut(s) 16
BsrFI RCCGGY 1 cut(s) 179
BsrI ACTGG 1 cut(s) 385
BssAI RCCGGY 1 cut(s) 179
BssMI GATC 1 cut(s) 120
Bst4CI ACNGT 3 cut(s) 80, 86, 666
Bst6I CTCTTC 2 cut(s) 16, 647
BstAPI GCANNNNNTGC 1 cut(s) 36
BstBAI YACGTR 1 cut(s) 474
BstDEI CTNAG 1 cut(s) 603
BstEII GGTNACC 1 cut(s) 86
BstF5I GGATG 2 cut(s) 159, 253
BstFNI CGCG 1 cut(s) 16
BstHHI GCGC 1 cut(s) 631
BstKTI GATC 1 cut(s) 123
BstMBI GATC 1 cut(s) 120
BstMWI GCNNNNNNNGC 3 cut(s) 36, 320, 485
BstPI GGTNACC 1 cut(s) 86
BstUI CGCG 1 cut(s) 16
BstV1I GCAGC 3 cut(s) 39, 219, 428
BstV2I GAAGAC 1 cut(s) 166
BtsCI GGATG 2 cut(s) 159, 253
BtsI GCAGTG 1 cut(s) 313
BtsIMutI CAGTG 2 cut(s) 313, 378
BtuMI TCGCGA 1 cut(s) 16
CfoI GCGC 1 cut(s) 631
Cfr10I RCCGGY 1 cut(s) 179
CseI GACGC 1 cut(s) 142
CviAII CATG 2 cut(s) 150, 639
CviJI RGCY 6 cut(s) 75, 216, 314, 479, 602, 645
CviKI_1 RGCY 6 cut(s) 75, 216, 314, 479, 602, 645
DdeI CTNAG 1 cut(s) 603
DpnI GATC 1 cut(s) 122
DpnII GATC 1 cut(s) 120
DraI TTTAAA 1 cut(s) 510
Eam1104I CTCTTC 2 cut(s) 16, 647
EarI CTCTTC 2 cut(s) 16, 647
Eco72I CACGTG 1 cut(s) 474
Eco91I GGTNACC 1 cut(s) 86
EcoO65I GGTNACC 1 cut(s) 86
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 2 cut(s) 153, 642
FaiI YATR 3 cut(s) 23, 151, 640
FatI CATG 2 cut(s) 149, 638
Fnu4HI GCNGC 4 cut(s) 28, 233, 417, 673
FokI GGATG 2 cut(s) 166, 260
Fsp4HI GCNGC 4 cut(s) 28, 233, 417, 673
FspBI CTAG 3 cut(s) 140, 264, 480
GlaI GCGC 1 cut(s) 630
GluI GCNGC 4 cut(s) 28, 233, 417, 673
GsuI CTGGAG 1 cut(s) 402
HapII CCGG 2 cut(s) 180, 197
HgaI GACGC 1 cut(s) 142
HhaI GCGC 1 cut(s) 631
Hin1II CATG 2 cut(s) 153, 642
Hin6I GCGC 1 cut(s) 629
HinP1I GCGC 1 cut(s) 629
HinfI GANTC 5 cut(s) 18, 221, 270, 374, 388
HpaII CCGG 2 cut(s) 180, 197
HphI GGTGA 2 cut(s) 98, 169
Hpy166II GTNNAC 1 cut(s) 228
Hpy188I TCNGA 2 cut(s) 220, 365
Hpy188III TCNNGA 3 cut(s) 15, 197, 334
Hpy8I GTNNAC 1 cut(s) 228
Hpy99I CGWCG 3 cut(s) 64, 109, 136
HpyAV CCTTC 1 cut(s) 512
HpyCH4III ACNGT 3 cut(s) 80, 86, 666
HpyCH4IV ACGT 1 cut(s) 473
HpyCH4V TGCA 6 cut(s) 4, 39, 206, 435, 464, 553
HpyF10VI GCNNNNNNNGC 3 cut(s) 36, 320, 485
HpyF3I CTNAG 1 cut(s) 603
HpySE526I ACGT 1 cut(s) 473
Hsp92II CATG 2 cut(s) 153, 642
HspAI GCGC 1 cut(s) 629
Kpn2I TCCGGA 1 cut(s) 196
Kzo9I GATC 1 cut(s) 120
LguI GCTCTTC 1 cut(s) 16
LmnI GCTCC 3 cut(s) 106, 221, 650
LpnPI CCDG 5 cut(s) 193, 210, 226, 326, 366
Lsp1109I GCAGC 3 cut(s) 39, 219, 428
LweI GCATC 3 cut(s) 13, 193, 316
MaeI CTAG 3 cut(s) 140, 264, 480
MaeII ACGT 1 cut(s) 473
MaeIII GTNAC 2 cut(s) 80, 86
MalI GATC 1 cut(s) 122
MboI GATC 1 cut(s) 120
MboII GAAGA 8 cut(s) 3, 61, 166, 212, 268, 279, 508, 664
MluCI AATT 5 cut(s) 32, 467, 505, 557, 595
MnlI CCTC 5 cut(s) 107, 136, 202, 359, 541
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 196
MseI TTAA 4 cut(s) 50, 165, 509, 543
MspI CCGG 2 cut(s) 180, 197
Mva1269I GAATGC 2 cut(s) 265, 631
MvnI CGCG 1 cut(s) 16
MwoI GCNNNNNNNGC 3 cut(s) 36, 320, 485
NdeII GATC 1 cut(s) 120
NlaIII CATG 2 cut(s) 153, 642
NlaIV GGNNCC 1 cut(s) 646
NmuCI GTSAC 1 cut(s) 86
NruI TCGCGA 1 cut(s) 16
NsiI ATGCAT 1 cut(s) 6
PciSI GCTCTTC 1 cut(s) 16
PcsI WCGNNNNNNNCGW 1 cut(s) 13
PctI GAATGC 2 cut(s) 265, 631
PfeI GAWTC 5 cut(s) 18, 221, 270, 374, 388
PkrI GCNGC 4 cut(s) 29, 234, 418, 674
PmaCI CACGTG 1 cut(s) 474
PmlI CACGTG 1 cut(s) 474
Ppu21I YACGTR 1 cut(s) 474
PspCI CACGTG 1 cut(s) 474
PspEI GGTNACC 1 cut(s) 86
PspN4I GGNNCC 1 cut(s) 646
RruI TCGCGA 1 cut(s) 16
SapI GCTCTTC 1 cut(s) 16
SaqAI TTAA 4 cut(s) 50, 165, 509, 543
SatI GCNGC 4 cut(s) 28, 233, 417, 673
Sau3AI GATC 1 cut(s) 120
SetI ASST 8 cut(s) 99, 141, 194, 218, 316, 476, 552, 585
SfaNI GCATC 3 cut(s) 13, 193, 316
Sse9I AATT 5 cut(s) 32, 467, 505, 557, 595
SsiI CCGC 4 cut(s) 91, 115, 245, 672
SspMI CTAG 3 cut(s) 140, 264, 480
TaaI ACNGT 3 cut(s) 80, 86, 666
TaiI ACGT 1 cut(s) 476
TaqI TCGA 2 cut(s) 238, 333
TasI AATT 5 cut(s) 32, 467, 505, 557, 595
TauI GCSGC 1 cut(s) 675
TfiI GAWTC 5 cut(s) 18, 221, 270, 374, 388
Tru1I TTAA 4 cut(s) 50, 165, 509, 543
Tru9I TTAA 4 cut(s) 50, 165, 509, 543
TscAI CASTG 2 cut(s) 313, 385
TseFI GTSAC 1 cut(s) 86
TseI GCWGC 3 cut(s) 27, 232, 416
Tsp45I GTSAC 1 cut(s) 86
TspDTI ATGAA 2 cut(s) 170, 269
TspGWI ACGGA 1 cut(s) 93
TspRI CASTG 2 cut(s) 313, 385
XapI RAATTY 3 cut(s) 32, 467, 505
XcmI CCANNNNNNNNNTGG 1 cut(s) 595
XspI CTAG 3 cut(s) 140, 264, 480
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.