pycom12g22470

DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0011499
Physical Location & Seq
Forward (+)
23343625 .. 23347309
3685 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1152 bp
ATGAAGAAAAATAAAATAATTATTTTGTTTATTCGACGGACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTAAAACTCCAAATGGAACCGAATGAAAACCAATTGAGCTCCTACTTCCAACACCCCACCACCACGACGGCCCCCGCCCCCGCCGCCCCTTCCACAGCCGCAACCACTCCCTCTCCGACCAATGGTCTCTTGCCCAACACCCACTCAACTTATGGCTCCCACATGGTGTACTCTCACTCAGTGCCCTCCTCGGCGGTGACCTCGCCACTTGAGCCCGCGAAAAGGAAGCGGGGGCGGCCCAGAAAGTACGGCACGCCGGAGCAGGCTCTGGCGGCCAAGAAGGCAGCGACGACGTCGTCTCACTCCTCGTCCTCCAAGGAGAAGAAGGACCAACACGGCGCCGTCTCGCCTTCGTTTTCTGGGTCGACCAAAAAATCTCAGCAGTTTTCTCTCGGCAATGCAGGGCAAGGTTTTACACCACATGTTTTAACTGTTGCTGCTGGGGAGGATGTGGGCCAGAAAATCATGTTTTTCATGCAACAGAGCAAGCGTGAAATATGCATTCTATCTGCTTCTGGTACCATCTCAAATGCATCTCTCCGCCAGCCAGCTACTTCGGGAGGCAACATTACATATGAGGGTCGCTTTGAGATTATTTCGTTGTCTGGATCTTATGTACGTACTGATCTTGGAGGGAGGGCTGGTGGGCTCAGTGTATGCCTATCTAATACAGATGGGCAAATCATCGGCGGTGGGGTTGGTGGTCCCCTGAAGGCTGCAGGCCCTGTGCAGGTTATTGTCGGAACATTTCAGATTGATGCCAAGAAGGATGCGACTGCTGGTGTAAAAGGTGATGCTTCTGCCGCCAAGTTACCATCGCCAGGGGAAATGATGAGCGGAGGCTTCCGCTCAACACTTGACTCTTCTGGAAGACCTCTTGTCAGGGGGAATGAAGATCAGCAACCTATGGGGGGAAGTCACTTCATGCTTCAGGGCATGCATGTCGCACCTTCACGGCCCACAGATTGGAGAGGTGGTCCAGATGCTAGAGGCACCGGTGCTTATGAATTGACAGGAAGAGGAGGTCGTGCACCACATCAATCTCCGGAGAACGGGGACTATGATCAAATTCAAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

384

Amino Acids

40.07

Weight (kDa)

9.74

Isoelectric Point (pI)

48.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 163 - 274 2.3e-23 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011499)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04590 AT3G04590
fragaria_vesca FvH4_6g02360 FvH4_6g02360
malus_domestica MD00G1114400.v1.1 MD12G1245000.v1.1
prunus_persica Prupe.6G347600_v2.0.a1 Prupe.6G347600_v2.0.a1
pyrus_communis pycom04g20220 pycom12g22470
rosa_chinensis RchiOBHm_Chr3g0450121
rosa_laevigata RLG00000025754
rosa_multiflora Rmu_ssc0000116.1_g000066
rosa_roxburghii Rroxscaffold_6G00427890
rosa_rugosa Rorug02G0625700
rosa_samantha Rh3AG024900 Rh3BG025500 Rh3CG024400 Rh3DG025500
rosa_wichuraiana Rw3G001990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 367
AatII GACGTC 1 cut(s) 368
Acc36I ACCTGC 1 cut(s) 793
Acc65I GGTACC 1 cut(s) 590
AccB1I GGYRCC 3 cut(s) 410, 590, 1064
AccB7I CCANNNNNTGG 1 cut(s) 1038
AccBSI CCGCTC 2 cut(s) 909, 921
AccI GTMKAC 1 cut(s) 437
AccII CGCG 1 cut(s) 290
AccIII TCCGGA 1 cut(s) 1117
AclWI GGATC 1 cut(s) 688
AcoI YGGCCR 1 cut(s) 345
AcsI RAATTY 1 cut(s) 1140
AcuI CTGAAG 2 cut(s) 803, 986
AcyI GRCGYC 2 cut(s) 365, 411
AdeI CACNNNGTG 2 cut(s) 238, 253
AfaI GTAC 5 cut(s) 242, 320, 592, 690, 694
AfiI CCNNNNNNNGG 7 cut(s) 194, 294, 802, 893, 983, 1038, 1124
AflIII ACRYGT 1 cut(s) 493
AgeI ACCGGT 1 cut(s) 1067
AgsI TTSAA 1 cut(s) 1145
AhdI GACNNNNNGTC 2 cut(s) 195, 950
AjnI CCWGG 1 cut(s) 892
AluBI AGCT 2 cut(s) 111, 623
AluI AGCT 2 cut(s) 111, 623
Alw21I GWGCWC 2 cut(s) 113, 1105
Alw26I GTCTC 3 cut(s) 203, 375, 421
Alw44I GTGCAC 1 cut(s) 1101
AlwI GGATC 1 cut(s) 688
AlwNI CAGNNNCTG 2 cut(s) 340, 797
Aor13HI TCCGGA 1 cut(s) 1117
AoxI GGCC 6 cut(s) 141, 308, 345, 526, 793, 1028
ApaLI GTGCAC 1 cut(s) 1101
ApeKI GCWGC 3 cut(s) 356, 509, 788
ApoI RAATTY 1 cut(s) 1140
AsiGI ACCGGT 1 cut(s) 1067
Asp718I GGTACC 1 cut(s) 590
AspLEI GCGC 1 cut(s) 413
AspS9I GGNCC 8 cut(s) 142, 309, 400, 526, 776, 794, 1029, 1049
AsuHPI GGTGA 2 cut(s) 280, 875
AvaII GGWCC 3 cut(s) 400, 776, 1049
BaeGI GKGCMC 2 cut(s) 258, 1105
BanI GGYRCC 3 cut(s) 410, 590, 1064
BanII GRGCYC 3 cut(s) 113, 288, 723
BbsI GAAGAC 1 cut(s) 949
Bbv12I GWGCWC 2 cut(s) 113, 1105
BbvI GCAGC 3 cut(s) 368, 496, 775
BccI CCATC 3 cut(s) 602, 740, 895
BceAI ACGGC 5 cut(s) 156, 337, 398, 424, 1043
BcgI CGANNNNNNTGC 2 cut(s) 997, 1031
BciT130I CCWGG 1 cut(s) 894
BclI TGATCA 1 cut(s) 1135
BcoDI GTCTC 3 cut(s) 203, 375, 421
BfaI CTAG 1 cut(s) 1059
BfmI CTRYAG 1 cut(s) 789
BfoI RGCGCY 1 cut(s) 414
BfuAI ACCTGC 1 cut(s) 793
BglI GCCNNNNNGGC 1 cut(s) 353
BisI GCNGC 8 cut(s) 156, 171, 308, 345, 357, 510, 789, 876
BlsI GCNGC 8 cut(s) 157, 172, 309, 346, 358, 511, 790, 877
Bme1390I CCNGG 1 cut(s) 894
Bme18I GGWCC 3 cut(s) 400, 776, 1049
BmeRI GACNNNNNGTC 2 cut(s) 195, 950
BmgT120I GGNCC 8 cut(s) 142, 309, 400, 526, 776, 794, 1029, 1049
BmiI GGNNCC 7 cut(s) 90, 144, 229, 412, 592, 778, 1066
BmrFI CCNGG 1 cut(s) 894
BmsI GCATC 5 cut(s) 614, 820, 832, 856, 1045
BpiI GAAGAC 1 cut(s) 949
BpuEI CTTGAG 1 cut(s) 302
BsaAI YACGTR 1 cut(s) 692
BsaHI GRCGYC 2 cut(s) 365, 411
BsaI GGTCTC 1 cut(s) 203
BsaJI CCNNGG 3 cut(s) 261, 387, 893
BsaWI WCCGGW 2 cut(s) 1067, 1117
BsaXI ACNNNNNCTCC 6 cut(s) 169, 199, 700, 730, 1033, 1063
Bsc4I CCNNNNNNNGG 7 cut(s) 194, 294, 802, 893, 983, 1038, 1124
Bse118I RCCGGY 1 cut(s) 1067
Bse3DI GCAATG 1 cut(s) 475
BseAI TCCGGA 1 cut(s) 1117
BseBI CCWGG 1 cut(s) 894
BseDI CCNNGG 3 cut(s) 261, 387, 893
BseGI GGATG 2 cut(s) 526, 847
BseLI CCNNNNNNNGG 7 cut(s) 194, 294, 802, 893, 983, 1038, 1124
BseMI GCAATG 1 cut(s) 475
BseMII CTCAG 3 cut(s) 264, 464, 736
BseRI GAGGAG 3 cut(s) 250, 367, 1107
BseSI GKGCMC 2 cut(s) 258, 1105
BseXI GCAGC 3 cut(s) 368, 496, 775
BseYI CCCAGC 1 cut(s) 512
BsgI GTGCAG 1 cut(s) 821
Bsh1236I CGCG 1 cut(s) 290
BshFI GGCC 6 cut(s) 143, 310, 347, 528, 795, 1030
BshNI GGYRCC 3 cut(s) 410, 590, 1064
BshTI ACCGGT 1 cut(s) 1067
BsiHKAI GWGCWC 2 cut(s) 113, 1105
BsiSI CCGG 3 cut(s) 329, 1068, 1118
BslFI GGGAC 2 cut(s) 762, 1142
BslI CCNNNNNNNGG 7 cut(s) 194, 294, 802, 893, 983, 1038, 1124
BsmAI GTCTC 3 cut(s) 203, 375, 421
BsmBI CGTCTC 2 cut(s) 375, 421
BsmFI GGGAC 2 cut(s) 762, 1142
BsmI GAATGC 1 cut(s) 573
BsnI GGCC 6 cut(s) 143, 310, 347, 528, 795, 1030
Bso31I GGTCTC 1 cut(s) 203
Bsp1286I GDGCHC 5 cut(s) 113, 258, 288, 723, 1105
Bsp13I TCCGGA 1 cut(s) 1117
Bsp143I GATC 4 cut(s) 680, 697, 967, 1135
BspANI GGCC 6 cut(s) 143, 310, 347, 528, 795, 1030
BspCNI CTCAG 3 cut(s) 263, 463, 735
BspEI TCCGGA 1 cut(s) 1117
BspFNI CGCG 1 cut(s) 290
BspLI GGNNCC 7 cut(s) 90, 144, 229, 412, 592, 778, 1066
BspMAI CTGCAG 1 cut(s) 793
BspMI ACCTGC 1 cut(s) 793
BspPI GGATC 1 cut(s) 688
BspT107I GGYRCC 3 cut(s) 410, 590, 1064
BspTNI GGTCTC 1 cut(s) 203
BsrBI CCGCTC 2 cut(s) 909, 921
BsrDI GCAATG 1 cut(s) 475
BsrFI RCCGGY 1 cut(s) 1067
BssAI RCCGGY 1 cut(s) 1067
BssECI CCNNGG 3 cut(s) 261, 387, 893
BssMI GATC 4 cut(s) 680, 697, 967, 1135
BssNI GRCGYC 2 cut(s) 365, 411
BssT1I CCWWGG 1 cut(s) 387
Bst2UI CCWGG 1 cut(s) 894
Bst4CI ACNGT 1 cut(s) 505
Bst6I CTCTTC 2 cut(s) 940, 1084
BstACI GRCGYC 2 cut(s) 365, 411
BstBAI YACGTR 1 cut(s) 692
BstC8I GCNNGC 8 cut(s) 288, 326, 336, 560, 617, 621, 793, 1010
BstDEI CTNAG 3 cut(s) 250, 450, 722
BstEII GGTNACC 1 cut(s) 268
BstF5I GGATG 2 cut(s) 526, 847
BstFNI CGCG 1 cut(s) 290
BstH2I RGCGCY 1 cut(s) 414
BstHHI GCGC 1 cut(s) 413
BstKTI GATC 4 cut(s) 683, 700, 970, 1138
BstMAI GTCTC 3 cut(s) 203, 375, 421
BstMBI GATC 4 cut(s) 680, 697, 967, 1135
BstMWI GCNNNNNNNGC 6 cut(s) 155, 283, 307, 344, 353, 875
BstNI CCWGG 1 cut(s) 894
BstNSI RCATGY 3 cut(s) 497, 1012, 1016
BstPI GGTNACC 1 cut(s) 268
BstSCI CCNGG 1 cut(s) 892
BstSFI CTRYAG 1 cut(s) 789
BstSLI GKGCMC 2 cut(s) 258, 1105
BstSNI TACGTA 1 cut(s) 692
BstUI CGCG 1 cut(s) 290
BstV1I GCAGC 3 cut(s) 368, 496, 775
BstV2I GAAGAC 1 cut(s) 949
BstX2I RGATCY 1 cut(s) 680
BstYI RGATCY 1 cut(s) 680
BsuRI GGCC 6 cut(s) 143, 310, 347, 528, 795, 1030
BtgZI GCGATG 1 cut(s) 873
BtsCI GGATG 2 cut(s) 526, 847
BtsIMutI CAGTG 2 cut(s) 258, 730
BveI ACCTGC 1 cut(s) 793
Cac8I GCNNGC 8 cut(s) 288, 326, 336, 560, 617, 621, 793, 1010
CaiI CAGNNNCTG 2 cut(s) 340, 797
CfoI GCGC 1 cut(s) 413
Cfr10I RCCGGY 1 cut(s) 1067
Cfr13I GGNCC 8 cut(s) 142, 309, 400, 526, 776, 794, 1029, 1049
Csp6I GTAC 5 cut(s) 241, 319, 591, 689, 693
CspAI ACCGGT 1 cut(s) 1067
CviAII CATG 7 cut(s) 235, 494, 538, 547, 997, 1009, 1013
CviQI GTAC 5 cut(s) 241, 319, 591, 689, 693
DdeI CTNAG 3 cut(s) 250, 450, 722
DinI GGCGCC 1 cut(s) 412
DpnI GATC 4 cut(s) 682, 699, 969, 1137
DpnII GATC 4 cut(s) 680, 697, 967, 1135
DraIII CACNNNGTG 2 cut(s) 238, 253
DrdI GACNNNNNNGTC 1 cut(s) 367
DriI GACNNNNNGTC 2 cut(s) 195, 950
DseDI GACNNNNNNGTC 1 cut(s) 367
EaeI YGGCCR 1 cut(s) 345
Eam1104I CTCTTC 2 cut(s) 940, 1084
Eam1105I GACNNNNNGTC 2 cut(s) 195, 950
EarI CTCTTC 2 cut(s) 940, 1084
EciI GGCGGA 1 cut(s) 602
Ecl136II GAGCTC 1 cut(s) 111
Eco105I TACGTA 1 cut(s) 692
Eco130I CCWWGG 1 cut(s) 387
Eco24I GRGCYC 3 cut(s) 113, 288, 723
Eco31I GGTCTC 1 cut(s) 203
Eco47I GGWCC 3 cut(s) 400, 776, 1049
Eco53kI GAGCTC 1 cut(s) 111
Eco57I CTGAAG 2 cut(s) 803, 986
Eco91I GGTNACC 1 cut(s) 268
EcoICRI GAGCTC 1 cut(s) 111
EcoO109I RGGNCCY 1 cut(s) 794
EcoO65I GGTNACC 1 cut(s) 268
EcoRII CCWGG 1 cut(s) 892
EcoT14I CCWWGG 1 cut(s) 387
EcoT22I ATGCAT 3 cut(s) 575, 607, 1014
EcoT38I GRGCYC 3 cut(s) 113, 288, 723
EgeI GGCGCC 1 cut(s) 412
EheI GGCGCC 1 cut(s) 412
ErhI CCWWGG 1 cut(s) 387
Esp3I CGTCTC 2 cut(s) 375, 421
FaeI CATG 7 cut(s) 238, 497, 541, 550, 1000, 1012, 1016
FaqI GGGAC 2 cut(s) 762, 1142
FatI CATG 7 cut(s) 234, 493, 537, 546, 996, 1008, 1012
FauI CCCGC 4 cut(s) 154, 160, 294, 295
FauNDI CATATG 1 cut(s) 646
FbaI TGATCA 1 cut(s) 1135
FblI GTMKAC 1 cut(s) 437
Fnu4HI GCNGC 8 cut(s) 156, 171, 308, 345, 357, 510, 789, 876
FokI GGATG 2 cut(s) 533, 854
FriOI GRGCYC 3 cut(s) 113, 288, 723
Fsp4HI GCNGC 8 cut(s) 156, 171, 308, 345, 357, 510, 789, 876
FspBI CTAG 1 cut(s) 1059
GlaI GCGC 1 cut(s) 412
GluI GCNGC 8 cut(s) 156, 171, 308, 345, 357, 510, 789, 876
GsaI CCCAGC 1 cut(s) 516
HaeII RGCGCY 1 cut(s) 414
HaeIII GGCC 6 cut(s) 143, 310, 347, 528, 795, 1030
HapII CCGG 3 cut(s) 329, 1068, 1118
HhaI GCGC 1 cut(s) 413
Hin1I GRCGYC 2 cut(s) 365, 411
Hin1II CATG 7 cut(s) 238, 497, 541, 550, 1000, 1012, 1016
Hin6I GCGC 1 cut(s) 411
HinP1I GCGC 1 cut(s) 411
HincII GTYRAC 1 cut(s) 438
HindII GTYRAC 1 cut(s) 438
HinfI GANTC 2 cut(s) 40, 932
HpaII CCGG 3 cut(s) 329, 1068, 1118
HphI GGTGA 2 cut(s) 280, 875
Hpy166II GTNNAC 3 cut(s) 241, 438, 1103
Hpy188I TCNGA 3 cut(s) 189, 815, 825
Hpy188III TCNNGA 6 cut(s) 630, 678, 939, 1052, 1118, 1145
Hpy8I GTNNAC 3 cut(s) 241, 438, 1103
Hpy99I CGWCG 5 cut(s) 39, 142, 364, 367, 370
HpyAV CCTTC 7 cut(s) 171, 346, 391, 432, 778, 832, 1032
HpyCH4III ACNGT 1 cut(s) 505
HpyCH4IV ACGT 2 cut(s) 365, 691
HpyCH4V TGCA 8 cut(s) 473, 550, 573, 605, 791, 802, 1012, 1103
HpyF10VI GCNNNNNNNGC 6 cut(s) 155, 283, 307, 344, 353, 875
HpyF3I CTNAG 3 cut(s) 250, 450, 722
HpySE526I ACGT 2 cut(s) 365, 691
Hsp92I GRCGYC 2 cut(s) 365, 411
Hsp92II CATG 7 cut(s) 238, 497, 541, 550, 1000, 1012, 1016
HspAI GCGC 1 cut(s) 411
KasI GGCGCC 1 cut(s) 410
Kpn2I TCCGGA 1 cut(s) 1117
KpnI GGTACC 1 cut(s) 594
Ksp22I TGATCA 1 cut(s) 1135
Kzo9I GATC 4 cut(s) 680, 697, 967, 1135
LmnI GCTCC 3 cut(s) 116, 233, 331
Lsp1109I GCAGC 3 cut(s) 368, 496, 775
LweI GCATC 5 cut(s) 614, 820, 832, 856, 1045
MaeI CTAG 1 cut(s) 1059
MaeII ACGT 2 cut(s) 365, 691
MaeIII GTNAC 3 cut(s) 268, 882, 989
MalI GATC 4 cut(s) 682, 699, 969, 1137
MbiI CCGCTC 2 cut(s) 909, 921
MboI GATC 4 cut(s) 680, 697, 967, 1135
MboII GAAGA 6 cut(s) 16, 406, 927, 954, 977, 1101
MfeI CAATTG 1 cut(s) 104
MflI RGATCY 1 cut(s) 680
MhlI GDGCHC 5 cut(s) 113, 258, 288, 723, 1105
MluCI AATT 4 cut(s) 18, 104, 1079, 1140
Mly113I GGCGCC 1 cut(s) 411
MlyI GAGTC 2 cut(s) 34, 926
MmeI TCCRAC 3 cut(s) 145, 212, 793
Mph1103I ATGCAT 3 cut(s) 575, 607, 1014
MroI TCCGGA 1 cut(s) 1117
MseI TTAA 1 cut(s) 500
MspI CCGG 3 cut(s) 329, 1068, 1118
MspR9I CCNGG 1 cut(s) 894
MunI CAATTG 1 cut(s) 104
Mva1269I GAATGC 1 cut(s) 573
MvaI CCWGG 1 cut(s) 894
MvnI CGCG 1 cut(s) 290
MwoI GCNNNNNNNGC 6 cut(s) 155, 283, 307, 344, 353, 875
NarI GGCGCC 1 cut(s) 411
NdeI CATATG 1 cut(s) 646
NdeII GATC 4 cut(s) 680, 697, 967, 1135
NlaIII CATG 7 cut(s) 238, 497, 541, 550, 1000, 1012, 1016
NlaIV GGNNCC 7 cut(s) 90, 144, 229, 412, 592, 778, 1066
NmeAIII GCCGAG 2 cut(s) 242, 444
NmuCI GTSAC 2 cut(s) 268, 989
NsiI ATGCAT 3 cut(s) 575, 607, 1014
NspI RCATGY 3 cut(s) 497, 1012, 1016
PaeI GCATGC 1 cut(s) 1012
PciI ACATGT 1 cut(s) 493
PctI GAATGC 1 cut(s) 573
PflFI GACNNNGTC 2 cut(s) 364, 367
PflMI CCANNNNNTGG 1 cut(s) 1038
PinAI ACCGGT 1 cut(s) 1067
PkrI GCNGC 8 cut(s) 157, 172, 309, 346, 358, 511, 790, 877
PleI GAGTC 2 cut(s) 34, 926
PluTI GGCGCC 1 cut(s) 414
PpsI GAGTC 2 cut(s) 34, 926
Ppu21I YACGTR 1 cut(s) 692
PscI ACATGT 1 cut(s) 493
Psp124BI GAGCTC 1 cut(s) 113
Psp6I CCWGG 1 cut(s) 892
PspEI GGTNACC 1 cut(s) 268
PspFI CCCAGC 1 cut(s) 512
PspGI CCWGG 1 cut(s) 892
PspN4I GGNNCC 7 cut(s) 90, 144, 229, 412, 592, 778, 1066
PspPI GGNCC 8 cut(s) 142, 309, 400, 526, 776, 794, 1029, 1049
PstI CTGCAG 1 cut(s) 793
PstNI CAGNNNCTG 2 cut(s) 340, 797
PsuI RGATCY 1 cut(s) 680
PsyI GACNNNGTC 2 cut(s) 364, 367
RsaI GTAC 5 cut(s) 242, 320, 592, 690, 694
RsaNI GTAC 5 cut(s) 241, 319, 591, 689, 693
SacI GAGCTC 1 cut(s) 113
SalI GTCGAC 1 cut(s) 436
SaqAI TTAA 1 cut(s) 500
SatI GCNGC 8 cut(s) 156, 171, 308, 345, 357, 510, 789, 876
Sau3AI GATC 4 cut(s) 680, 697, 967, 1135
Sau96I GGNCC 8 cut(s) 142, 309, 400, 526, 776, 794, 1029, 1049
SchI GAGTC 2 cut(s) 34, 926
ScrFI CCNGG 1 cut(s) 894
SduI GDGCHC 5 cut(s) 113, 258, 288, 723, 1105
SfaNI GCATC 5 cut(s) 614, 820, 832, 856, 1045
SfcI CTRYAG 1 cut(s) 789
SfoI GGCGCC 1 cut(s) 412
SgrAI CRCCGGYG 1 cut(s) 1067
SinI GGWCC 3 cut(s) 400, 776, 1049
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
SnaBI TACGTA 1 cut(s) 692
SphI GCATGC 1 cut(s) 1012
Sse9I AATT 4 cut(s) 18, 104, 1079, 1140
SspDI GGCGCC 1 cut(s) 410
SspMI CTAG 1 cut(s) 1059
SstI GAGCTC 1 cut(s) 113
StyD4I CCNGG 1 cut(s) 892
StyI CCWWGG 1 cut(s) 387
TaaI ACNGT 1 cut(s) 505
TaiI ACGT 2 cut(s) 368, 694
TaqI TCGA 2 cut(s) 34, 437
TasI AATT 4 cut(s) 18, 104, 1079, 1140
TatI WGTACW 1 cut(s) 240
TauI GCSGC 5 cut(s) 158, 173, 310, 347, 878
Tru1I TTAA 1 cut(s) 500
Tru9I TTAA 1 cut(s) 500
TscAI CASTG 2 cut(s) 258, 730
TseFI GTSAC 2 cut(s) 268, 989
TseI GCWGC 3 cut(s) 356, 509, 788
Tsp45I GTSAC 2 cut(s) 268, 989
TspDTI ATGAA 6 cut(s) 17, 111, 535, 978, 985, 1092
TspGWI ACGGA 1 cut(s) 52
TspRI CASTG 2 cut(s) 258, 730
Tth111I GACNNNGTC 2 cut(s) 364, 367
Van91I CCANNNNNTGG 1 cut(s) 1038
VneI GTGCAC 1 cut(s) 1101
VpaK11BI GGWCC 3 cut(s) 400, 776, 1049
XapI RAATTY 1 cut(s) 1140
XceI RCATGY 3 cut(s) 497, 1012, 1016
XcmI CCANNNNNNNNNTGG 1 cut(s) 221
XmiI GTMKAC 1 cut(s) 437
XspI CTAG 1 cut(s) 1059
ZraI GACGTC 1 cut(s) 366
Zsp2I ATGCAT 3 cut(s) 575, 607, 1014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.