pycom13g00810

Aldo/keto reductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0015614
Physical Location & Seq
Reverse (-)
509301 .. 512023
2723 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1089 bp
ATGAAGGGAAAGTTTGGAACACCAGGAAATTACCTTGTGTTAAACACAGGGGATAGAATTCCTGCCATTGGACTTGGGACATGGCAGAGTGGCGGAGACCTTTGTGTTCAAGCCGTAAAAACGGCGTTATCGGTGGGTTACCGCCATATAGATTGTGCACATCTTTATGGTAATGAGATCGAAGTTGGGGAAGCATTAGCTGAAGCATTTAGGGGTTCACTGAAGAGGGAAGACGTTTTCTTGACTTCTAATCTCTACTGCACAATGAATCCTCTCAATAAAATTGAGAATTATGTTCGAGATTCTCTTAAGAATCTTGGAGTTTCGTATTTGGATCTATATTTGATGCATTGGCCTGATAGCTCAGCGTTTGGTGATGCTACTGATCCGCCTTCAAAGTCTGGGAGCGAGTATAGGCAATTCTTGAATCGGTCGAAGAAAGCATGGAAAGCTATGGAAGGTCTGGTTGAGATGGGTCTGGTGAGGGCTATTGGGGTCAGCAATTTCAGTGTTTCGCAAATCAAACAATTGCTTAAATTTGCAAAGATTGTACCCGCTGTCAATCAGGTGGAGTTGCACCCCTTTTGGAGGCAAGATGAACTTGTAAAGTTCTGTCAATTGAAAGGTATTCATGTGTCTGCTCACACACCCTTAGGAGTCCCAACTTCTACTCCTGGACCATCTGATAGCAGTTCAGGTGGGGAAGATGAACCTGGAACTCCTCGCATATCATTCAGGAGGTCAAGGTCTGTGCATGGTCCTATGCTGAAAATATCAACAGTTGCAGAGATAGCAGACCGACACAGAAAAACACCTGAGCAGGTTATTTTGCGTTGGGGGCTGCAAAGAGGAACCAGTGTGCTGCCTTGTAGTCTGAAGCCTGATAGGATTAGAAAAAACATAGACATCTTCAGTTGGTCTCTATCAGATGATGAGTGGAACCGCTTGAACCAAAGATCGATCGAGCCCCAGGTATGCTTGTTTGGAAATGGTCCTTTGAATAATCTTTCGAAGAGTGGGTTTATGTCTGGAAGTGGTCCACTTCAAGCTGTGCATGAGATGGAAGATGACATGGAATCCAATGCCTGA

Protein Analysis

363

Amino Acids

40.05

Weight (kDa)

8.15

Isoelectric Point (pI)

38.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 22 - 318 2.6e-52 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015614)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g36371
malus_domestica MD13G1009500.v1.1 MD16G1007700.v1.1
prunus_persica Prupe.1G343100_v2.0.a1
pyrus_communis pycom13g00810 pycom16g00760
rosa_chinensis RchiOBHm_Chr4g0446121
rosa_laevigata RLG00000005690
rosa_multiflora Rmu_sc0012061.1_g000005
rosa_roxburghii Rroxscaffold_5G00386520
rosa_rugosa Rorug04G0368000
rosa_samantha Rh4AG429600 Rh4BG439100 Rh4CG456400 Rh4DG437500
rosa_wichuraiana Rw4G036710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 811
AciI CCGC 5 cut(s) 93, 142, 389, 555, 943
AclWI GGATC 2 cut(s) 342, 380
AcsI RAATTY 2 cut(s) 57, 536
AcuI CTGAAG 4 cut(s) 222, 242, 895, 896
AfaI GTAC 1 cut(s) 552
AfiI CCNNNNNNNGG 2 cut(s) 68, 588
AflII CTTAAG 1 cut(s) 308
AgsI TTSAA 7 cut(s) 110, 396, 427, 622, 949, 1000, 1046
AjnI CCWGG 4 cut(s) 22, 673, 712, 969
AluBI AGCT 4 cut(s) 200, 363, 452, 1049
AluI AGCT 4 cut(s) 200, 363, 452, 1049
Alw21I GWGCWC 1 cut(s) 160
Alw26I GTCTC 2 cut(s) 90, 924
Alw44I GTGCAC 1 cut(s) 156
AlwI GGATC 2 cut(s) 342, 380
AoxI GGCC 1 cut(s) 353
ApaLI GTGCAC 1 cut(s) 156
ApeKI GCWGC 2 cut(s) 841, 862
ApoI RAATTY 2 cut(s) 57, 536
AspS9I GGNCC 4 cut(s) 677, 758, 992, 1037
AsuHPI GGTGA 2 cut(s) 386, 493
AsuII TTCGAA 1 cut(s) 1010
AvaII GGWCC 4 cut(s) 677, 758, 992, 1037
AxyI CCTNAGG 1 cut(s) 652
BaeGI GKGCMC 1 cut(s) 160
BanII GRGCYC 1 cut(s) 969
BbsI GAAGAC 1 cut(s) 237
Bbv12I GWGCWC 1 cut(s) 160
BbvI GCAGC 2 cut(s) 828, 849
BccI CCATC 3 cut(s) 466, 688, 1054
BceAI ACGGC 2 cut(s) 98, 138
BciT130I CCWGG 4 cut(s) 24, 675, 714, 971
BcoDI GTCTC 2 cut(s) 90, 924
BfrI CTTAAG 1 cut(s) 308
BfuAI ACCTGC 1 cut(s) 811
BisI GCNGC 2 cut(s) 842, 863
BlpI GCTNAGC 1 cut(s) 364
BlsI GCNGC 2 cut(s) 843, 864
Bme1390I CCNGG 4 cut(s) 24, 675, 714, 971
Bme18I GGWCC 4 cut(s) 677, 758, 992, 1037
BmgT120I GGNCC 4 cut(s) 677, 758, 992, 1037
BmiI GGNNCC 2 cut(s) 853, 941
BmrFI CCNGG 4 cut(s) 24, 675, 714, 971
BmsI GCATC 2 cut(s) 336, 367
BpiI GAAGAC 1 cut(s) 237
Bpu10I CCTNAGC 1 cut(s) 816
Bpu1102I GCTNAGC 1 cut(s) 364
Bpu14I TTCGAA 1 cut(s) 1010
Bsa29I ATCGAT 1 cut(s) 959
BsaI GGTCTC 2 cut(s) 90, 924
BsaJI CCNNGG 1 cut(s) 969
BsaXI ACNNNNNCTCC 2 cut(s) 655, 685
Bsc4I CCNNNNNNNGG 2 cut(s) 68, 588
Bse1I ACTGG 1 cut(s) 855
Bse21I CCTNAGG 1 cut(s) 652
BseBI CCWGG 4 cut(s) 24, 675, 714, 971
BseCI ATCGAT 1 cut(s) 959
BseDI CCNNGG 1 cut(s) 969
BseLI CCNNNNNNNGG 2 cut(s) 68, 588
BseMII CTCAG 2 cut(s) 378, 807
BseNI ACTGG 1 cut(s) 855
BseRI GAGGAG 1 cut(s) 711
BseSI GKGCMC 1 cut(s) 160
BseXI GCAGC 2 cut(s) 828, 849
BsgI GTGCAG 1 cut(s) 244
Bsh1285I CGRYCG 2 cut(s) 434, 963
BshFI GGCC 1 cut(s) 355
BshVI ATCGAT 1 cut(s) 959
BsiEI CGRYCG 2 cut(s) 434, 963
BsiHKAI GWGCWC 1 cut(s) 160
BslFI GGGAC 2 cut(s) 91, 644
BslI CCNNNNNNNGG 2 cut(s) 68, 588
BsmAI GTCTC 2 cut(s) 90, 924
BsmFI GGGAC 2 cut(s) 91, 644
BsnI GGCC 1 cut(s) 355
Bso31I GGTCTC 2 cut(s) 90, 924
Bsp119I TTCGAA 1 cut(s) 1010
Bsp1286I GDGCHC 2 cut(s) 160, 969
Bsp143I GATC 5 cut(s) 177, 334, 385, 956, 960
Bsp1720I GCTNAGC 1 cut(s) 364
BspACI CCGC 5 cut(s) 93, 142, 389, 555, 943
BspANI GGCC 1 cut(s) 355
BspCNI CTCAG 2 cut(s) 377, 808
BspDI ATCGAT 1 cut(s) 959
BspLI GGNNCC 2 cut(s) 853, 941
BspMI ACCTGC 1 cut(s) 811
BspPI GGATC 2 cut(s) 342, 380
BspT104I TTCGAA 1 cut(s) 1010
BspTI CTTAAG 1 cut(s) 308
BspTNI GGTCTC 2 cut(s) 90, 924
BsrI ACTGG 1 cut(s) 855
BssECI CCNNGG 1 cut(s) 969
BssMI GATC 5 cut(s) 177, 334, 385, 956, 960
Bst2UI CCWGG 4 cut(s) 24, 675, 714, 971
Bst4CI ACNGT 1 cut(s) 781
Bst6I CTCTTC 2 cut(s) 218, 1007
BstAFI CTTAAG 1 cut(s) 308
BstBI TTCGAA 1 cut(s) 1010
BstDEI CTNAG 3 cut(s) 364, 652, 816
BstEII GGTNACC 1 cut(s) 137
BstENI CCTNNNNNAGG 1 cut(s) 586
BstKTI GATC 5 cut(s) 180, 337, 388, 959, 963
BstMAI GTCTC 2 cut(s) 90, 924
BstMBI GATC 5 cut(s) 177, 334, 385, 956, 960
BstMCI CGRYCG 2 cut(s) 434, 963
BstMWI GCNNNNNNNGC 3 cut(s) 449, 791, 838
BstNI CCWGG 4 cut(s) 24, 675, 714, 971
BstPI GGTNACC 1 cut(s) 137
BstSCI CCNGG 4 cut(s) 22, 673, 712, 969
BstSLI GKGCMC 1 cut(s) 160
BstV1I GCAGC 2 cut(s) 828, 849
BstV2I GAAGAC 1 cut(s) 237
BstX2I RGATCY 1 cut(s) 334
BstYI RGATCY 1 cut(s) 334
Bsu15I ATCGAT 1 cut(s) 959
Bsu36I CCTNAGG 1 cut(s) 652
BsuRI GGCC 1 cut(s) 355
BsuTUI ATCGAT 1 cut(s) 959
BtsIMutI CAGTG 3 cut(s) 218, 514, 862
BveI ACCTGC 1 cut(s) 811
Cfr13I GGNCC 4 cut(s) 677, 758, 992, 1037
ClaI ATCGAT 1 cut(s) 959
Csp6I GTAC 1 cut(s) 551
CviAII CATG 6 cut(s) 81, 444, 632, 755, 1055, 1072
CviQI GTAC 1 cut(s) 551
DdeI CTNAG 3 cut(s) 364, 652, 816
DpnI GATC 5 cut(s) 179, 336, 387, 958, 962
DpnII GATC 5 cut(s) 177, 334, 385, 956, 960
Eam1104I CTCTTC 2 cut(s) 218, 1007
EarI CTCTTC 2 cut(s) 218, 1007
EciI GGCGGA 2 cut(s) 108, 378
Eco24I GRGCYC 1 cut(s) 969
Eco31I GGTCTC 2 cut(s) 90, 924
Eco47I GGWCC 4 cut(s) 677, 758, 992, 1037
Eco57I CTGAAG 4 cut(s) 222, 242, 895, 896
Eco81I CCTNAGG 1 cut(s) 652
Eco91I GGTNACC 1 cut(s) 137
EcoNI CCTNNNNNAGG 1 cut(s) 586
EcoO65I GGTNACC 1 cut(s) 137
EcoRI GAATTC 1 cut(s) 57
EcoRII CCWGG 4 cut(s) 22, 673, 712, 969
EcoT22I ATGCAT 1 cut(s) 351
EcoT38I GRGCYC 1 cut(s) 969
FaeI CATG 6 cut(s) 84, 447, 635, 758, 1058, 1075
FalI AAGNNNNNCTT 2 cut(s) 585, 617
FaqI GGGAC 2 cut(s) 91, 644
FatI CATG 6 cut(s) 80, 443, 631, 754, 1054, 1071
FauI CCCGC 1 cut(s) 562
Fnu4HI GCNGC 2 cut(s) 842, 863
FriOI GRGCYC 1 cut(s) 969
Fsp4HI GCNGC 2 cut(s) 842, 863
GluI GCNGC 2 cut(s) 842, 863
HaeIII GGCC 1 cut(s) 355
Hin1II CATG 6 cut(s) 84, 447, 635, 758, 1058, 1075
HinfI GANTC 6 cut(s) 268, 302, 313, 427, 657, 1076
HphI GGTGA 2 cut(s) 386, 493
Hpy166II GTNNAC 3 cut(s) 158, 218, 1040
Hpy188I TCNGA 3 cut(s) 685, 876, 928
Hpy188III TCNNGA 5 cut(s) 241, 299, 424, 736, 1029
Hpy8I GTNNAC 3 cut(s) 158, 218, 1040
HpyAV CCTTC 2 cut(s) 402, 452
HpyCH4III ACNGT 1 cut(s) 781
HpyCH4IV ACGT 1 cut(s) 234
HpyCH4V TGCA 9 cut(s) 158, 261, 349, 542, 577, 754, 785, 844, 1054
HpyF10VI GCNNNNNNNGC 3 cut(s) 449, 791, 838
HpyF3I CTNAG 3 cut(s) 364, 652, 816
HpySE526I ACGT 1 cut(s) 234
Hsp92II CATG 6 cut(s) 84, 447, 635, 758, 1058, 1075
Kzo9I GATC 5 cut(s) 177, 334, 385, 956, 960
LmnI GCTCC 1 cut(s) 405
Lsp1109I GCAGC 2 cut(s) 828, 849
LweI GCATC 2 cut(s) 336, 367
MaeII ACGT 1 cut(s) 234
MaeIII GTNAC 1 cut(s) 137
MalI GATC 5 cut(s) 179, 336, 387, 958, 962
MboI GATC 5 cut(s) 177, 334, 385, 956, 960
MboII GAAGA 7 cut(s) 235, 242, 448, 716, 901, 1024, 1076
MfeI CAATTG 2 cut(s) 527, 617
MflI RGATCY 1 cut(s) 334
MhlI GDGCHC 2 cut(s) 160, 969
MluCI AATT 9 cut(s) 28, 57, 282, 289, 419, 502, 527, 536, 617
MlyI GAGTC 1 cut(s) 666
MnlI CCTC 7 cut(s) 219, 282, 477, 582, 732, 732, 842
Mph1103I ATGCAT 1 cut(s) 351
MseI TTAA 3 cut(s) 41, 309, 534
MslI CAYNNNNRTG 1 cut(s) 165
MspA1I CMGCKG 1 cut(s) 557
MspCI CTTAAG 1 cut(s) 308
MspR9I CCNGG 4 cut(s) 24, 675, 714, 971
MunI CAATTG 2 cut(s) 527, 617
MvaI CCWGG 4 cut(s) 24, 675, 714, 971
MwoI GCNNNNNNNGC 3 cut(s) 449, 791, 838
NdeII GATC 5 cut(s) 177, 334, 385, 956, 960
NlaIII CATG 6 cut(s) 84, 447, 635, 758, 1058, 1075
NlaIV GGNNCC 2 cut(s) 853, 941
NsiI ATGCAT 1 cut(s) 351
NspV TTCGAA 1 cut(s) 1010
PfeI GAWTC 5 cut(s) 268, 302, 313, 427, 1076
PfoI TCCNGGA 1 cut(s) 673
PkrI GCNGC 2 cut(s) 843, 864
Ple19I CGATCG 1 cut(s) 963
PleI GAGTC 1 cut(s) 665
PpsI GAGTC 1 cut(s) 665
Psp6I CCWGG 4 cut(s) 22, 673, 712, 969
PspEI GGTNACC 1 cut(s) 137
PspGI CCWGG 4 cut(s) 22, 673, 712, 969
PspN4I GGNNCC 2 cut(s) 853, 941
PspPI GGNCC 4 cut(s) 677, 758, 992, 1037
PsuI RGATCY 1 cut(s) 334
PvuI CGATCG 1 cut(s) 963
RsaI GTAC 1 cut(s) 552
RsaNI GTAC 1 cut(s) 551
RseI CAYNNNNRTG 1 cut(s) 165
SaqAI TTAA 3 cut(s) 41, 309, 534
SatI GCNGC 2 cut(s) 842, 863
Sau3AI GATC 5 cut(s) 177, 334, 385, 956, 960
Sau96I GGNCC 4 cut(s) 677, 758, 992, 1037
SchI GAGTC 1 cut(s) 666
ScrFI CCNGG 4 cut(s) 24, 675, 714, 971
SduI GDGCHC 2 cut(s) 160, 969
SfaNI GCATC 2 cut(s) 336, 367
SfuI TTCGAA 1 cut(s) 1010
SinI GGWCC 4 cut(s) 677, 758, 992, 1037
SmiMI CAYNNNNRTG 1 cut(s) 165
SmlI CTYRAG 1 cut(s) 308
SmoI CTYRAG 1 cut(s) 308
Sse9I AATT 9 cut(s) 28, 57, 282, 289, 419, 502, 527, 536, 617
SsiI CCGC 5 cut(s) 93, 142, 389, 555, 943
StyD4I CCNGG 4 cut(s) 22, 673, 712, 969
TaaI ACNGT 1 cut(s) 781
TaiI ACGT 1 cut(s) 237
TaqI TCGA 6 cut(s) 180, 298, 434, 959, 963, 1010
TaqII GACCGA 2 cut(s) 420, 813
TasI AATT 9 cut(s) 28, 57, 282, 289, 419, 502, 527, 536, 617
TfiI GAWTC 5 cut(s) 268, 302, 313, 427, 1076
Tru1I TTAA 3 cut(s) 41, 309, 534
Tru9I TTAA 3 cut(s) 41, 309, 534
TscAI CASTG 3 cut(s) 225, 514, 862
TseI GCWGC 2 cut(s) 841, 862
TspDTI ATGAA 5 cut(s) 17, 281, 612, 620, 723
TspRI CASTG 3 cut(s) 225, 514, 862
Vha464I CTTAAG 1 cut(s) 308
VneI GTGCAC 1 cut(s) 156
VpaK11BI GGWCC 4 cut(s) 677, 758, 992, 1037
XagI CCTNNNNNAGG 1 cut(s) 586
XapI RAATTY 2 cut(s) 57, 536
Zsp2I ATGCAT 1 cut(s) 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.