Rh4CG456400

Aldo/keto reductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0015614
Physical Location & Seq
Forward (+)
68861464 .. 68866304
4841 bp
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UTR
Exon/CDS
Intron
Rh4CG456400.1

Sequence Viewer

Length: 831 bp
ATGAAAGGAAAGGTAGGATTACCAGGGAATTACTTTGTGTTGAACACAGGAGATAAAATTCCAGCCATTGGACTTGGGACATGGCAGAGTGGTGGAGACCTTTGTGTTCAAGCTGTGAAAACAGCATTATCAGTAGGTTACCGACACATAGATTGTGCGCATCTTTATGGTAATGAGGTTGAAGTTGGTGAGGCATTAGCTGAAGCATTTAAGGGTTCACTGAAGAGGGAAGATGTTTTCTTGACTTCTAAGCTCTACTGCACAATGAATTCTCTCAATAAAATTGAGAATTATGTTAGAGTTTCTCTGAAGAACCTCGGCGTTTCGTATTTGGATCTGTATTTGATGCATTGGCCTGACAGCTCTGCGTTTGGTGATGCTACTGATCCTCCTTCGAAATCCGGGAGTGAGTATAGGCAGTTCTTGAATCGGCTGAAGAAAGCATGGAAAGCTATGGAAGGACTGGTTGAGTTGGGTCTGGTTAGAGCTATTGGGGTCAGCAATTTCAGTGTTGAGCAGATCAAACAATTGCTTAAATTTGCAAAAATTGTACCCGCAGTCAATCAGGTGGAGTTGCACCCTTTTTGGAGGCAAGATGAACTTGTAAAGTTATGTCAATTGAAAGGTATTCATGTATCAGCTCACACACCCTTAGGAGTCCCAGCTTCAAATCCTGGACCATCTGATAGCAGTTCAGGTGGGGAAGATGAGCCTGGAACTCCTCGCATATCATTCAGAAGGTCAAGGTCTGTGCATGGGCCTATGCTGAAATTGTCAACTGTTGAACAAATAGCAGATAGACACAGAAAAACACCTGAGCAGGTACTTTAG

Protein Analysis

276

Amino Acids

30.35

Weight (kDa)

8.93

Isoelectric Point (pI)

29.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 22 - 275 1.2e-43 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015614)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g36371
malus_domestica MD13G1009500.v1.1 MD16G1007700.v1.1
prunus_persica Prupe.1G343100_v2.0.a1
pyrus_communis pycom13g00810 pycom16g00760
rosa_chinensis RchiOBHm_Chr4g0446121
rosa_laevigata RLG00000005690
rosa_multiflora Rmu_sc0012061.1_g000005
rosa_roxburghii Rroxscaffold_5G00386520
rosa_rugosa Rorug04G0368000
rosa_samantha Rh4AG429600 Rh4BG439100 Rh4CG456400 Rh4DG437500
rosa_wichuraiana Rw4G036710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 159
Acc36I ACCTGC 1 cut(s) 811
AccB7I CCANNNNNTGG 1 cut(s) 68
AciI CCGC 1 cut(s) 555
AclWI GGATC 2 cut(s) 342, 380
AcsI RAATTY 3 cut(s) 57, 268, 536
AcuI CTGAAG 4 cut(s) 222, 242, 329, 455
AfaI GTAC 2 cut(s) 552, 825
AfiI CCNNNNNNNGG 1 cut(s) 68
AgsI TTSAA 7 cut(s) 43, 110, 182, 427, 622, 669, 785
AjnI CCWGG 3 cut(s) 22, 673, 712
AluBI AGCT 8 cut(s) 113, 200, 253, 363, 452, 488, 641, 665
AluI AGCT 8 cut(s) 113, 200, 253, 363, 452, 488, 641, 665
Alw26I GTCTC 1 cut(s) 90
AlwI GGATC 2 cut(s) 342, 380
AoxI GGCC 2 cut(s) 353, 758
ApoI RAATTY 3 cut(s) 57, 268, 536
AspLEI GCGC 1 cut(s) 160
AspS9I GGNCC 2 cut(s) 677, 758
AsuC2I CCSGG 1 cut(s) 403
AsuHPI GGTGA 2 cut(s) 200, 386
AsuII TTCGAA 1 cut(s) 395
AvaII GGWCC 1 cut(s) 677
AxyI CCTNAGG 1 cut(s) 652
BccI CCATC 1 cut(s) 688
BciT130I CCWGG 3 cut(s) 24, 675, 714
BcnI CCSGG 1 cut(s) 403
BcoDI GTCTC 1 cut(s) 90
BfuAI ACCTGC 1 cut(s) 811
Bme1390I CCNGG 4 cut(s) 24, 403, 675, 714
Bme18I GGWCC 1 cut(s) 677
BmgT120I GGNCC 2 cut(s) 677, 758
BmrFI CCNGG 4 cut(s) 24, 403, 675, 714
BmsI GCATC 3 cut(s) 169, 336, 367
Bpu10I CCTNAGC 1 cut(s) 816
Bpu14I TTCGAA 1 cut(s) 395
BpuMI CCSGG 1 cut(s) 403
BsaI GGTCTC 1 cut(s) 90
BsaJI CCNNGG 2 cut(s) 23, 316
BsaXI ACNNNNNCTCC 2 cut(s) 373, 403
Bsc4I CCNNNNNNNGG 1 cut(s) 68
Bse1I ACTGG 1 cut(s) 468
Bse21I CCTNAGG 1 cut(s) 652
BseBI CCWGG 3 cut(s) 24, 675, 714
BseDI CCNNGG 2 cut(s) 23, 316
BseLI CCNNNNNNNGG 1 cut(s) 68
BseMII CTCAG 1 cut(s) 807
BseNI ACTGG 1 cut(s) 468
BseRI GAGGAG 1 cut(s) 711
BseYI CCCAGC 1 cut(s) 661
BsgI GTGCAG 1 cut(s) 244
BshFI GGCC 2 cut(s) 355, 760
BsiSI CCGG 1 cut(s) 402
BslFI GGGAC 2 cut(s) 91, 644
BslI CCNNNNNNNGG 1 cut(s) 68
BsmAI GTCTC 1 cut(s) 90
BsmFI GGGAC 2 cut(s) 91, 644
BsnI GGCC 2 cut(s) 355, 760
Bso31I GGTCTC 1 cut(s) 90
Bsp119I TTCGAA 1 cut(s) 395
Bsp143I GATC 3 cut(s) 334, 385, 519
BspACI CCGC 1 cut(s) 555
BspANI GGCC 2 cut(s) 355, 760
BspCNI CTCAG 1 cut(s) 808
BspMI ACCTGC 1 cut(s) 811
BspPI GGATC 2 cut(s) 342, 380
BspT104I TTCGAA 1 cut(s) 395
BspTNI GGTCTC 1 cut(s) 90
BsrI ACTGG 1 cut(s) 468
BssECI CCNNGG 2 cut(s) 23, 316
BssMI GATC 3 cut(s) 334, 385, 519
Bst2UI CCWGG 3 cut(s) 24, 675, 714
Bst4CI ACNGT 1 cut(s) 781
Bst6I CTCTTC 1 cut(s) 218
BstBI TTCGAA 1 cut(s) 395
BstDEI CTNAG 3 cut(s) 249, 652, 816
BstEII GGTNACC 1 cut(s) 137
BstHHI GCGC 1 cut(s) 160
BstKTI GATC 3 cut(s) 337, 388, 522
BstMAI GTCTC 1 cut(s) 90
BstMBI GATC 3 cut(s) 334, 385, 519
BstMWI GCNNNNNNNGC 1 cut(s) 449
BstNI CCWGG 3 cut(s) 24, 675, 714
BstPI GGTNACC 1 cut(s) 137
BstSCI CCNGG 4 cut(s) 22, 401, 673, 712
BstX2I RGATCY 1 cut(s) 334
BstYI RGATCY 1 cut(s) 334
Bsu36I CCTNAGG 1 cut(s) 652
BsuRI GGCC 2 cut(s) 355, 760
BtsIMutI CAGTG 2 cut(s) 218, 514
BveI ACCTGC 1 cut(s) 811
CfoI GCGC 1 cut(s) 160
Cfr13I GGNCC 2 cut(s) 677, 758
Csp6I GTAC 2 cut(s) 551, 824
CviAII CATG 4 cut(s) 81, 444, 632, 755
CviQI GTAC 2 cut(s) 551, 824
DdeI CTNAG 3 cut(s) 249, 652, 816
DpnI GATC 3 cut(s) 336, 387, 521
DpnII GATC 3 cut(s) 334, 385, 519
Eam1104I CTCTTC 1 cut(s) 218
EarI CTCTTC 1 cut(s) 218
Eco31I GGTCTC 1 cut(s) 90
Eco47I GGWCC 1 cut(s) 677
Eco57I CTGAAG 4 cut(s) 222, 242, 329, 455
Eco81I CCTNAGG 1 cut(s) 652
Eco91I GGTNACC 1 cut(s) 137
EcoO65I GGTNACC 1 cut(s) 137
EcoRI GAATTC 1 cut(s) 268
EcoRII CCWGG 3 cut(s) 22, 673, 712
EcoT22I ATGCAT 1 cut(s) 351
FaeI CATG 4 cut(s) 84, 447, 635, 758
FalI AAGNNNNNCTT 2 cut(s) 585, 617
FaqI GGGAC 2 cut(s) 91, 644
FatI CATG 4 cut(s) 80, 443, 631, 754
FauI CCCGC 1 cut(s) 562
FspAI RTGCGCAY 1 cut(s) 159
FspI TGCGCA 1 cut(s) 159
GlaI GCGC 1 cut(s) 159
GsaI CCCAGC 1 cut(s) 665
HaeIII GGCC 2 cut(s) 355, 760
HapII CCGG 1 cut(s) 402
HhaI GCGC 1 cut(s) 160
Hin1II CATG 4 cut(s) 84, 447, 635, 758
Hin6I GCGC 1 cut(s) 158
HinP1I GCGC 1 cut(s) 158
HincII GTYRAC 1 cut(s) 777
HindII GTYRAC 1 cut(s) 777
HinfI GANTC 2 cut(s) 427, 657
HpaII CCGG 1 cut(s) 402
HphI GGTGA 2 cut(s) 200, 386
Hpy166II GTNNAC 2 cut(s) 218, 777
Hpy188I TCNGA 3 cut(s) 309, 685, 737
Hpy188III TCNNGA 2 cut(s) 241, 424
Hpy8I GTNNAC 2 cut(s) 218, 777
HpyAV CCTTC 3 cut(s) 402, 452, 732
HpyCH4III ACNGT 1 cut(s) 781
HpyCH4V TGCA 5 cut(s) 261, 349, 542, 577, 754
HpyF10VI GCNNNNNNNGC 1 cut(s) 449
HpyF3I CTNAG 3 cut(s) 249, 652, 816
Hsp92II CATG 4 cut(s) 84, 447, 635, 758
HspAI GCGC 1 cut(s) 158
Kzo9I GATC 3 cut(s) 334, 385, 519
LweI GCATC 3 cut(s) 169, 336, 367
MaeIII GTNAC 1 cut(s) 137
MalI GATC 3 cut(s) 336, 387, 521
MboI GATC 3 cut(s) 334, 385, 519
MboII GAAGA 5 cut(s) 235, 242, 322, 448, 716
MfeI CAATTG 2 cut(s) 527, 617
MflI RGATCY 1 cut(s) 334
MlyI GAGTC 1 cut(s) 666
MnlI CCTC 7 cut(s) 169, 184, 219, 326, 399, 582, 732
Mph1103I ATGCAT 1 cut(s) 351
MseI TTAA 2 cut(s) 210, 534
MslI CAYNNNNRTG 1 cut(s) 165
MspI CCGG 1 cut(s) 402
MspR9I CCNGG 4 cut(s) 24, 403, 675, 714
MunI CAATTG 2 cut(s) 527, 617
MvaI CCWGG 3 cut(s) 24, 675, 714
MwoI GCNNNNNNNGC 1 cut(s) 449
NciI CCSGG 1 cut(s) 403
NdeII GATC 3 cut(s) 334, 385, 519
NlaIII CATG 4 cut(s) 84, 447, 635, 758
NmeAIII GCCGAG 1 cut(s) 297
NsbI TGCGCA 1 cut(s) 159
NsiI ATGCAT 1 cut(s) 351
NspV TTCGAA 1 cut(s) 395
PfeI GAWTC 1 cut(s) 427
PflMI CCANNNNNTGG 1 cut(s) 68
PfoI TCCNGGA 2 cut(s) 401, 673
PleI GAGTC 1 cut(s) 665
PpsI GAGTC 1 cut(s) 665
Psp6I CCWGG 3 cut(s) 22, 673, 712
PspEI GGTNACC 1 cut(s) 137
PspFI CCCAGC 1 cut(s) 661
PspGI CCWGG 3 cut(s) 22, 673, 712
PspPI GGNCC 2 cut(s) 677, 758
PsrI GAACNNNNNNTAC 2 cut(s) 404, 436
PsuI RGATCY 1 cut(s) 334
RsaI GTAC 2 cut(s) 552, 825
RsaNI GTAC 2 cut(s) 551, 824
RseI CAYNNNNRTG 1 cut(s) 165
SaqAI TTAA 2 cut(s) 210, 534
Sau3AI GATC 3 cut(s) 334, 385, 519
Sau96I GGNCC 2 cut(s) 677, 758
SchI GAGTC 1 cut(s) 666
ScrFI CCNGG 4 cut(s) 24, 403, 675, 714
SfaNI GCATC 3 cut(s) 169, 336, 367
SfuI TTCGAA 1 cut(s) 395
SinI GGWCC 1 cut(s) 677
SmiMI CAYNNNNRTG 1 cut(s) 165
SsiI CCGC 1 cut(s) 555
StyD4I CCNGG 4 cut(s) 22, 401, 673, 712
TaaI ACNGT 1 cut(s) 781
TaqI TCGA 1 cut(s) 395
TfiI GAWTC 1 cut(s) 427
Tru1I TTAA 2 cut(s) 210, 534
Tru9I TTAA 2 cut(s) 210, 534
TscAI CASTG 2 cut(s) 225, 514
TspDTI ATGAA 4 cut(s) 17, 281, 612, 620
TspRI CASTG 2 cut(s) 225, 514
Van91I CCANNNNNTGG 1 cut(s) 68
VpaK11BI GGWCC 1 cut(s) 677
XapI RAATTY 3 cut(s) 57, 268, 536
Zsp2I ATGCAT 1 cut(s) 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.