RchiOBHm_Chr4g0446121

Aldo/keto reductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0015614
Physical Location & Seq
Forward (+)
66403196 .. 66408334
5139 bp
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UTR
Exon/CDS
Intron
PRQ41364

Sequence Viewer

Length: 1089 bp
ATGAAAGGAAAGGTAGGATTACCAGGGAATTACTTTGTGTTGAACACAGGAGATAAAATTCCAGCCATTGGACTTGGGACATGGCAGAGTGGTGGAGACCTTTGTGTTCAAGCTGTGAAAACAGCATTATCAGTAGGTTACCGACACATAGATTGTGCACATCTTTATGGTAATGAGGTTGAAGTTGGTGAGGCATTAGCTGAAGCATTCAAGGGTTCACTGAAGAGGGAAGATGTTTTCTTGACTTCTAAGCTCTACTGCACAATGAATTCTCTCAATAAAATTGAGAATTATGTTAGAGTTTCTCTGAAGAACCTCGGCGTTTCGTATTTGGATCTGTATTTGATGCATTGGCCTGACAGCTCTGCGTTTGGTGACGCTACTGATCCTCCTTCGAAATCCGGGAGTGAGTATAGGCAGTTCTTGAATCGGCTGAAGAAAGCATGGAAAGCTATGGAAGGACTGGTTGAGTTGGGTCTGGTTAGAGCTATTGGGGTCAGCAATTTCAGCGTTGAGCAGATCAAACAATTGCTTAAATTTGCAAAAATTGTACCTGCAGTCAATCAGGTGGAGTTGCACCCTTTTTGGAGGCAAGATGAACTTGTAAAGTTATGTCAATTGAAAGGTATTCATGTATCAGCTCACACACCCTTAGGAGTCCCAGCTTCAAATCCTGGACCATCTGATAGCAGTTCAGGTGGGGAAGATGAGCCCGGAACTCCTCGCATATCATTCAGAAGGTCAAGGTCTGTGCATGGGCCTATGCTGAAATTGTCAACTGTTGAACAAATAGCAGATAGACACAGAAAAACACCTGAGCAGGTAATTTTGCGTTGGGGGCTGCAAAGAGGAACCAGCGTCCTACCATGTAGCTTGAAGCCAGATAGGATAAGAAAAAACATAGACATCTTCAGTTGGTCTCTGACAGACGATGAGTGGAATCGGTTGAATCACATTGAACCACAGGTATGCTTATTTGGAAACAGCCCTTTGAATACTCTTTCAGAGAGTGGCTTTGTGTCTGAAAGTGGATGCGGTCCTCTTCAAGCTGTGCATGAGATGGAAGATGACATGGAATCCAATGCCTGA

Protein Analysis

362

Amino Acids

40.02

Weight (kDa)

6.8

Isoelectric Point (pI)

36.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 22 - 319 8.2e-55 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015614)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g36371
malus_domestica MD13G1009500.v1.1 MD16G1007700.v1.1
prunus_persica Prupe.1G343100_v2.0.a1
pyrus_communis pycom13g00810 pycom16g00760
rosa_chinensis RchiOBHm_Chr4g0446121
rosa_laevigata RLG00000005690
rosa_multiflora Rmu_sc0012061.1_g000005
rosa_roxburghii Rroxscaffold_5G00386520
rosa_rugosa Rorug04G0368000
rosa_samantha Rh4AG429600 Rh4BG439100 Rh4CG456400 Rh4DG437500
rosa_wichuraiana Rw4G036710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 562, 811
AccB7I CCANNNNNTGG 1 cut(s) 68
AciI CCGC 1 cut(s) 1035
AclWI GGATC 2 cut(s) 342, 380
AcsI RAATTY 3 cut(s) 57, 268, 536
AcuI CTGAAG 5 cut(s) 222, 242, 329, 455, 895
AfaI GTAC 1 cut(s) 552
AfiI CCNNNNNNNGG 1 cut(s) 68
AjnI CCWGG 2 cut(s) 22, 673
Alw21I GWGCWC 1 cut(s) 160
Alw26I GTCTC 2 cut(s) 90, 924
Alw44I GTGCAC 1 cut(s) 156
AlwI GGATC 2 cut(s) 342, 380
AoxI GGCC 2 cut(s) 353, 758
ApaLI GTGCAC 1 cut(s) 156
ApeKI GCWGC 1 cut(s) 841
ApoI RAATTY 3 cut(s) 57, 268, 536
AspS9I GGNCC 3 cut(s) 677, 758, 1037
AsuC2I CCSGG 2 cut(s) 403, 714
AsuHPI GGTGA 2 cut(s) 200, 386
AsuII TTCGAA 1 cut(s) 395
AvaII GGWCC 2 cut(s) 677, 1037
AxyI CCTNAGG 1 cut(s) 652
BaeGI GKGCMC 1 cut(s) 160
BanII GRGCYC 1 cut(s) 714
Bbv12I GWGCWC 1 cut(s) 160
BbvI GCAGC 1 cut(s) 828
BccI CCATC 2 cut(s) 688, 1054
BciT130I CCWGG 2 cut(s) 24, 675
BcnI CCSGG 2 cut(s) 403, 714
BcoDI GTCTC 2 cut(s) 90, 924
BfmI CTRYAG 1 cut(s) 555
BfuAI ACCTGC 2 cut(s) 562, 811
BisI GCNGC 1 cut(s) 842
BlsI GCNGC 1 cut(s) 843
Bme1390I CCNGG 4 cut(s) 24, 403, 675, 714
Bme18I GGWCC 2 cut(s) 677, 1037
BmgT120I GGNCC 3 cut(s) 677, 758, 1037
BmiI GGNNCC 1 cut(s) 853
BmrFI CCNGG 4 cut(s) 24, 403, 675, 714
BmsI GCATC 2 cut(s) 336, 1022
Bpu10I CCTNAGC 1 cut(s) 816
Bpu14I TTCGAA 1 cut(s) 395
BpuMI CCSGG 2 cut(s) 403, 714
BsaI GGTCTC 2 cut(s) 90, 924
BsaJI CCNNGG 2 cut(s) 23, 316
BsaXI ACNNNNNCTCC 2 cut(s) 373, 403
Bsc4I CCNNNNNNNGG 1 cut(s) 68
Bse1I ACTGG 1 cut(s) 468
Bse21I CCTNAGG 1 cut(s) 652
BseBI CCWGG 2 cut(s) 24, 675
BseDI CCNNGG 2 cut(s) 23, 316
BseGI GGATG 1 cut(s) 1037
BseLI CCNNNNNNNGG 1 cut(s) 68
BseMII CTCAG 1 cut(s) 807
BseNI ACTGG 1 cut(s) 468
BseRI GAGGAG 1 cut(s) 711
BseSI GKGCMC 1 cut(s) 160
BseXI GCAGC 1 cut(s) 828
BseYI CCCAGC 1 cut(s) 661
BsgI GTGCAG 1 cut(s) 244
BshFI GGCC 2 cut(s) 355, 760
BsiHKAI GWGCWC 1 cut(s) 160
BsiSI CCGG 2 cut(s) 402, 714
BslFI GGGAC 2 cut(s) 91, 644
BslI CCNNNNNNNGG 1 cut(s) 68
BsmAI GTCTC 2 cut(s) 90, 924
BsmFI GGGAC 2 cut(s) 91, 644
BsmI GAATGC 1 cut(s) 206
BsnI GGCC 2 cut(s) 355, 760
Bso31I GGTCTC 2 cut(s) 90, 924
Bsp119I TTCGAA 1 cut(s) 395
Bsp1286I GDGCHC 2 cut(s) 160, 714
Bsp143I GATC 3 cut(s) 334, 385, 519
BspACI CCGC 1 cut(s) 1035
BspANI GGCC 2 cut(s) 355, 760
BspCNI CTCAG 1 cut(s) 808
BspLI GGNNCC 1 cut(s) 853
BspMAI CTGCAG 1 cut(s) 559
BspMI ACCTGC 2 cut(s) 562, 811
BspPI GGATC 2 cut(s) 342, 380
BspT104I TTCGAA 1 cut(s) 395
BspTNI GGTCTC 2 cut(s) 90, 924
BsrI ACTGG 1 cut(s) 468
BssECI CCNNGG 2 cut(s) 23, 316
BssMI GATC 3 cut(s) 334, 385, 519
Bst2UI CCWGG 2 cut(s) 24, 675
Bst4CI ACNGT 1 cut(s) 781
Bst6I CTCTTC 2 cut(s) 218, 1047
BstBI TTCGAA 1 cut(s) 395
BstDEI CTNAG 3 cut(s) 249, 652, 816
BstEII GGTNACC 1 cut(s) 137
BstF5I GGATG 1 cut(s) 1037
BstKTI GATC 3 cut(s) 337, 388, 522
BstMAI GTCTC 2 cut(s) 90, 924
BstMBI GATC 3 cut(s) 334, 385, 519
BstMWI GCNNNNNNNGC 3 cut(s) 449, 507, 838
BstNI CCWGG 2 cut(s) 24, 675
BstPI GGTNACC 1 cut(s) 137
BstSCI CCNGG 4 cut(s) 22, 401, 673, 712
BstSFI CTRYAG 1 cut(s) 555
BstSLI GKGCMC 1 cut(s) 160
BstV1I GCAGC 1 cut(s) 828
BstX2I RGATCY 1 cut(s) 334
BstYI RGATCY 1 cut(s) 334
Bsu36I CCTNAGG 1 cut(s) 652
BsuRI GGCC 2 cut(s) 355, 760
BtsCI GGATG 1 cut(s) 1037
BtsIMutI CAGTG 1 cut(s) 218
BveI ACCTGC 2 cut(s) 562, 811
Cfr13I GGNCC 3 cut(s) 677, 758, 1037
CseI GACGC 2 cut(s) 386, 847
Csp6I GTAC 1 cut(s) 551
CviAII CATG 7 cut(s) 81, 444, 632, 755, 867, 1055, 1072
CviQI GTAC 1 cut(s) 551
DdeI CTNAG 3 cut(s) 249, 652, 816
DpnI GATC 3 cut(s) 336, 387, 521
DpnII GATC 3 cut(s) 334, 385, 519
Eam1104I CTCTTC 2 cut(s) 218, 1047
EarI CTCTTC 2 cut(s) 218, 1047
Eco24I GRGCYC 1 cut(s) 714
Eco31I GGTCTC 2 cut(s) 90, 924
Eco47I GGWCC 2 cut(s) 677, 1037
Eco57I CTGAAG 5 cut(s) 222, 242, 329, 455, 895
Eco81I CCTNAGG 1 cut(s) 652
Eco91I GGTNACC 1 cut(s) 137
EcoO65I GGTNACC 1 cut(s) 137
EcoRI GAATTC 1 cut(s) 268
EcoRII CCWGG 2 cut(s) 22, 673
EcoT22I ATGCAT 1 cut(s) 351
EcoT38I GRGCYC 1 cut(s) 714
FaeI CATG 7 cut(s) 84, 447, 635, 758, 870, 1058, 1075
FalI AAGNNNNNCTT 2 cut(s) 585, 617
FaqI GGGAC 2 cut(s) 91, 644
FatI CATG 7 cut(s) 80, 443, 631, 754, 866, 1054, 1071
Fnu4HI GCNGC 1 cut(s) 842
FokI GGATG 1 cut(s) 1044
FriOI GRGCYC 1 cut(s) 714
Fsp4HI GCNGC 1 cut(s) 842
GluI GCNGC 1 cut(s) 842
GsaI CCCAGC 1 cut(s) 665
HaeIII GGCC 2 cut(s) 355, 760
HapII CCGG 2 cut(s) 402, 714
HgaI GACGC 2 cut(s) 386, 847
Hin1II CATG 7 cut(s) 84, 447, 635, 758, 870, 1058, 1075
HincII GTYRAC 1 cut(s) 777
HindII GTYRAC 1 cut(s) 777
HinfI GANTC 5 cut(s) 427, 657, 940, 949, 1076
HpaII CCGG 2 cut(s) 402, 714
HphI GGTGA 2 cut(s) 200, 386
Hpy166II GTNNAC 3 cut(s) 158, 218, 777
Hpy188I TCNGA 6 cut(s) 309, 685, 737, 924, 1006, 1024
Hpy188III TCNNGA 2 cut(s) 241, 424
Hpy8I GTNNAC 3 cut(s) 158, 218, 777
HpyAV CCTTC 3 cut(s) 402, 452, 732
HpyCH4III ACNGT 1 cut(s) 781
HpyCH4V TGCA 9 cut(s) 158, 261, 349, 542, 557, 577, 754, 844, 1054
HpyF10VI GCNNNNNNNGC 3 cut(s) 449, 507, 838
HpyF3I CTNAG 3 cut(s) 249, 652, 816
Hsp92II CATG 7 cut(s) 84, 447, 635, 758, 870, 1058, 1075
Kzo9I GATC 3 cut(s) 334, 385, 519
Lsp1109I GCAGC 1 cut(s) 828
LweI GCATC 2 cut(s) 336, 1022
MaeIII GTNAC 2 cut(s) 137, 374
MalI GATC 3 cut(s) 336, 387, 521
MboI GATC 3 cut(s) 334, 385, 519
MboII GAAGA 8 cut(s) 235, 242, 322, 448, 716, 901, 1034, 1076
MfeI CAATTG 2 cut(s) 527, 617
MflI RGATCY 1 cut(s) 334
MhlI GDGCHC 2 cut(s) 160, 714
MlyI GAGTC 1 cut(s) 666
MnlI CCTC 9 cut(s) 169, 184, 219, 326, 399, 582, 732, 842, 1050
Mph1103I ATGCAT 1 cut(s) 351
MseI TTAA 1 cut(s) 534
MslI CAYNNNNRTG 2 cut(s) 165, 967
MspI CCGG 2 cut(s) 402, 714
MspR9I CCNGG 4 cut(s) 24, 403, 675, 714
MunI CAATTG 2 cut(s) 527, 617
Mva1269I GAATGC 1 cut(s) 206
MvaI CCWGG 2 cut(s) 24, 675
MwoI GCNNNNNNNGC 3 cut(s) 449, 507, 838
NciI CCSGG 2 cut(s) 403, 714
NdeII GATC 3 cut(s) 334, 385, 519
NlaIII CATG 7 cut(s) 84, 447, 635, 758, 870, 1058, 1075
NlaIV GGNNCC 1 cut(s) 853
NmeAIII GCCGAG 1 cut(s) 297
NmuCI GTSAC 1 cut(s) 374
NsiI ATGCAT 1 cut(s) 351
NspV TTCGAA 1 cut(s) 395
PctI GAATGC 1 cut(s) 206
PfeI GAWTC 4 cut(s) 427, 940, 949, 1076
PflMI CCANNNNNTGG 1 cut(s) 68
PfoI TCCNGGA 2 cut(s) 401, 673
PkrI GCNGC 1 cut(s) 843
PleI GAGTC 1 cut(s) 665
PpsI GAGTC 1 cut(s) 665
Psp6I CCWGG 2 cut(s) 22, 673
PspEI GGTNACC 1 cut(s) 137
PspFI CCCAGC 1 cut(s) 661
PspGI CCWGG 2 cut(s) 22, 673
PspN4I GGNNCC 1 cut(s) 853
PspPI GGNCC 3 cut(s) 677, 758, 1037
PsrI GAACNNNNNNTAC 2 cut(s) 404, 436
PstI CTGCAG 1 cut(s) 559
PsuI RGATCY 1 cut(s) 334
RsaI GTAC 1 cut(s) 552
RsaNI GTAC 1 cut(s) 551
RseI CAYNNNNRTG 2 cut(s) 165, 967
SaqAI TTAA 1 cut(s) 534
SatI GCNGC 1 cut(s) 842
Sau3AI GATC 3 cut(s) 334, 385, 519
Sau96I GGNCC 3 cut(s) 677, 758, 1037
SchI GAGTC 1 cut(s) 666
ScrFI CCNGG 4 cut(s) 24, 403, 675, 714
SduI GDGCHC 2 cut(s) 160, 714
SfaNI GCATC 2 cut(s) 336, 1022
SfcI CTRYAG 1 cut(s) 555
SfuI TTCGAA 1 cut(s) 395
SinI GGWCC 2 cut(s) 677, 1037
SmiMI CAYNNNNRTG 2 cut(s) 165, 967
SsiI CCGC 1 cut(s) 1035
StyD4I CCNGG 4 cut(s) 22, 401, 673, 712
TaaI ACNGT 1 cut(s) 781
TaqI TCGA 1 cut(s) 395
TfiI GAWTC 4 cut(s) 427, 940, 949, 1076
Tru1I TTAA 1 cut(s) 534
Tru9I TTAA 1 cut(s) 534
TscAI CASTG 1 cut(s) 225
TseFI GTSAC 1 cut(s) 374
TseI GCWGC 1 cut(s) 841
Tsp45I GTSAC 1 cut(s) 374
TspDTI ATGAA 4 cut(s) 17, 281, 612, 620
TspRI CASTG 1 cut(s) 225
Van91I CCANNNNNTGG 1 cut(s) 68
VneI GTGCAC 1 cut(s) 156
VpaK11BI GGWCC 2 cut(s) 677, 1037
XapI RAATTY 3 cut(s) 57, 268, 536
Zsp2I ATGCAT 1 cut(s) 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.