pycom13g00860

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0007044
Physical Location & Seq
Forward (+)
538129 .. 540523
2395 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1410 bp
ATGGGGAGTAGGATCCAGCAGCACTACGCCATGAGATCGCCGCCCGCCAATTCCTATATTGGCAACGCTCTTCACGACCTCAACACCGCCGACGCTGCTCGCCCCGCCGCAGAGATCGACTCCATCGATGGTGATTCCGTCGCTGAAGATACCCACCTCCGCGAGGACGACGGCTCCGGCGCCGTTGTCGACGGCTGTATGCACGAAGCGTATACCAATTCTTTGCCTCTTCACTCGGTTCGCCTTGGAGTTGAAGACGATCGCTCCGCCAGCAGTGGCGGCGGCTCTTCCACTTCCAGGGCTCCCTACTGTCTCTTGTCACTTCAAGATGTTTCGCCTATCGAATCGGCTAGGGCACGGTTTCTGCAACTGATTGTGGATCATTTTATTAGTGAACATGTGGTTGAAGTGCCTAATTCTGAGGCTGCTTCTTCTGCTGAGTACAATGGTGCTCTGTCTCACTCTGGGCAAGAAAAACTAAACAAGAGGAAGCCTGGAGAGGTGCGGTATGAAGGCGACCCCAGGCTGGCTTTGCCATTGATGTATGTGGCAAATGTGTACCAAACTTTGGTTAACGATGCGAATATTAGGCTTTCAAGCTTGAGTGGATTTCGAGAGAAGACAATTGGGGTTGCCCTTGAAGCATCTGGTGGGTTGTACAGATGCTTGGCGAAGAAGTTTCCCAAAAAAGGATCGCGTACATTTAGAAGAAGAGAGTTGGCCACATCCGTTGAAACGAGGACGAGATTTCCAGAGTTGGTCATACAAGAAGAGAAGCGAGTTCGTTTTGTGGTAGTCAATGGTTTGGATATTGTTGAAAAGCCTACTAGGATGCCTACTGATGATGCTGAGTGGTTCAAACGGTTGACTGGTCGGGATGAAGTAGCAGTCTATGCTCGAGACTTTAAGTTCTACTCTCCAAGACACAAGTATAGACGTGTTGCATCAAACTCCTCGCCTAATAGTATCGCCAGTTTACCTACATTTCCTGGCACTGATAATTCTTCCATGTTGGCTGCTGCTCAGGGATTCCGCTCCGCAAGTGAACCACAGAATCAGCAAACGACTCCTTCGTGTAAGCATCATATGCAACCCTTGTCGCACCAGCCTCAGTTTCAGCACCAGACTCATCACCAATCCATCAACCAAAGTCCGCATGCTGTTCATTATTCTCAAAACCATCAATGCGGTGCTACCTCTCACCTGCCTGAAATCGCTCATGCTCACCATTCCCCAACCATGTCGCAACACATGGTTTGCTTACAACCCCTCACTGGAGGCCATGTAGGGGGACGTTTGCATGTGTTGCCATCCAGTCCTGCCAAGTTTTGCGATGAATGTGGGGCGCCGTACTTGAGAGAAACCTCCAAGTTCTGCTCTGAATGCGGCGTTAAGAGGTTGGGAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

470

Amino Acids

51.63

Weight (kDa)

7.71

Isoelectric Point (pI)

60.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1212
Acc36I ACCTGC 1 cut(s) 1212
AccB1I GGYRCC 2 cut(s) 179, 1345
AccB7I CCANNNNNTGG 1 cut(s) 568
AccBSI CCGCTC 1 cut(s) 1035
AccI GTMKAC 2 cut(s) 189, 212
AccII CGCG 2 cut(s) 162, 697
AclWI GGATC 4 cut(s) 7, 20, 387, 700
AcoI YGGCCR 1 cut(s) 720
AcuI CTGAAG 1 cut(s) 165
AcyI GRCGYC 2 cut(s) 180, 1346
AfaI GTAC 5 cut(s) 443, 560, 659, 700, 1352
AfiI CCNNNNNNNGG 7 cut(s) 163, 297, 526, 568, 689, 1274, 1288
AflIII ACRYGT 2 cut(s) 397, 937
AgsI TTSAA 8 cut(s) 254, 326, 407, 597, 641, 734, 818, 859
AjiI CACGTC 1 cut(s) 938
AjnI CCWGG 4 cut(s) 296, 493, 521, 988
AluBI AGCT 1 cut(s) 600
AluI AGCT 1 cut(s) 600
Alw21I GWGCWC 1 cut(s) 454
Alw26I GTCTC 3 cut(s) 317, 462, 894
AlwI GGATC 4 cut(s) 7, 20, 387, 700
Ama87I CYCGRG 1 cut(s) 897
AoxI GGCC 2 cut(s) 720, 1279
ApeKI GCWGC 5 cut(s) 19, 95, 425, 1016, 1019
AspLEI GCGC 2 cut(s) 182, 1348
AsuHPI GGTGA 4 cut(s) 143, 1124, 1193, 1217
AvaI CYCGRG 1 cut(s) 897
BaeGI GKGCMC 1 cut(s) 358
BalI TGGCCA 1 cut(s) 722
BamHI GGATCC 1 cut(s) 12
BanI GGYRCC 2 cut(s) 179, 1345
BanII GRGCYC 1 cut(s) 304
BbsI GAAGAC 2 cut(s) 261, 626
Bbv12I GWGCWC 1 cut(s) 454
BbvI GCAGC 5 cut(s) 31, 82, 412, 1003, 1006
BccI CCATC 5 cut(s) 122, 131, 1148, 1188, 1318
BceAI ACGGC 4 cut(s) 167, 187, 208, 1333
BciT130I CCWGG 4 cut(s) 298, 495, 523, 990
BcoDI GTCTC 3 cut(s) 317, 462, 894
BfaI CTAG 2 cut(s) 351, 828
BfoI RGCGCY 2 cut(s) 183, 1349
BfuAI ACCTGC 1 cut(s) 1212
Bme1390I CCNGG 4 cut(s) 298, 495, 523, 990
BmeT110I CYCGRG 1 cut(s) 897
BmgBI CACGTC 1 cut(s) 938
BmiI GGNNCC 5 cut(s) 14, 175, 181, 303, 1347
BmrFI CCNGG 4 cut(s) 298, 495, 523, 990
BmsI GCATC 7 cut(s) 568, 653, 653, 822, 835, 953, 1090
BpiI GAAGAC 2 cut(s) 261, 626
BplI GAGNNNNNCTC 2 cut(s) 104, 136
BpmI CTGGAG 2 cut(s) 516, 1296
Bpu10I CCTNAGC 1 cut(s) 1023
BpuEI CTTGAG 2 cut(s) 622, 1375
Bsa29I ATCGAT 1 cut(s) 126
BsaHI GRCGYC 2 cut(s) 180, 1346
BsaJI CCNNGG 3 cut(s) 244, 297, 521
BsaXI ACNNNNNCTCC 6 cut(s) 158, 188, 248, 278, 1269, 1299
Bsc4I CCNNNNNNNGG 7 cut(s) 163, 297, 526, 568, 689, 1274, 1288
Bse1I ACTGG 4 cut(s) 874, 972, 1279, 1314
BseBI CCWGG 4 cut(s) 298, 495, 523, 990
BseCI ATCGAT 1 cut(s) 126
BseDI CCNNGG 3 cut(s) 244, 297, 521
BseGI GGATG 4 cut(s) 725, 837, 883, 1310
BseLI CCNNNNNNNGG 7 cut(s) 163, 297, 526, 568, 689, 1274, 1288
BseMII CTCAG 5 cut(s) 411, 429, 840, 1037, 1124
BseNI ACTGG 4 cut(s) 874, 972, 1279, 1314
BseRI GAGGAG 1 cut(s) 943
BseSI GKGCMC 1 cut(s) 358
BseXI GCAGC 5 cut(s) 31, 82, 412, 1003, 1006
Bsh1236I CGCG 2 cut(s) 162, 697
Bsh1285I CGRYCG 1 cut(s) 262
BshFI GGCC 2 cut(s) 722, 1281
BshNI GGYRCC 2 cut(s) 179, 1345
BshVI ATCGAT 1 cut(s) 126
BsiEI CGRYCG 1 cut(s) 262
BsiHKAI GWGCWC 1 cut(s) 454
BsiHKCI CYCGRG 1 cut(s) 897
BsiSI CCGG 1 cut(s) 177
BslFI GGGAC 1 cut(s) 1305
BslI CCNNNNNNNGG 7 cut(s) 163, 297, 526, 568, 689, 1274, 1288
BsmAI GTCTC 3 cut(s) 317, 462, 894
BsmFI GGGAC 1 cut(s) 1305
BsmI GAATGC 1 cut(s) 1388
BsnI GGCC 2 cut(s) 722, 1281
BsoBI CYCGRG 1 cut(s) 897
Bsp1286I GDGCHC 3 cut(s) 304, 358, 454
Bsp1407I TGTACA 1 cut(s) 657
Bsp143I GATC 6 cut(s) 12, 35, 114, 259, 379, 692
BspANI GGCC 2 cut(s) 722, 1281
BspCNI CTCAG 5 cut(s) 412, 430, 841, 1036, 1123
BspDI ATCGAT 1 cut(s) 126
BspFNI CGCG 2 cut(s) 162, 697
BspLI GGNNCC 5 cut(s) 14, 175, 181, 303, 1347
BspMI ACCTGC 1 cut(s) 1212
BspPI GGATC 4 cut(s) 7, 20, 387, 700
BspQI GCTCTTC 2 cut(s) 75, 292
BspT107I GGYRCC 2 cut(s) 179, 1345
BsrBI CCGCTC 1 cut(s) 1035
BsrGI TGTACA 1 cut(s) 657
BsrI ACTGG 4 cut(s) 874, 972, 1279, 1314
BssECI CCNNGG 3 cut(s) 244, 297, 521
BssMI GATC 6 cut(s) 12, 35, 114, 259, 379, 692
BssNAI GTATAC 1 cut(s) 213
BssNI GRCGYC 2 cut(s) 180, 1346
BssT1I CCWWGG 1 cut(s) 244
Bst1107I GTATAC 1 cut(s) 213
Bst2UI CCWGG 4 cut(s) 298, 495, 523, 990
Bst4CI ACNGT 3 cut(s) 311, 360, 864
Bst6I CTCTTC 5 cut(s) 75, 234, 292, 706, 765
BstACI GRCGYC 2 cut(s) 180, 1346
BstAPI GCANNNNNTGC 3 cut(s) 893, 1087, 1306
BstAUI TGTACA 1 cut(s) 657
BstC8I GCNNGC 5 cut(s) 45, 100, 271, 528, 1158
BstDEI CTNAG 5 cut(s) 420, 438, 849, 1023, 1110
BstENI CCTNNNNNAGG 1 cut(s) 161
BstF5I GGATG 4 cut(s) 725, 837, 883, 1310
BstFNI CGCG 2 cut(s) 162, 697
BstH2I RGCGCY 2 cut(s) 183, 1349
BstHHI GCGC 2 cut(s) 182, 1348
BstKTI GATC 6 cut(s) 15, 38, 117, 262, 382, 695
BstMAI GTCTC 3 cut(s) 317, 462, 894
BstMBI GATC 6 cut(s) 12, 35, 114, 259, 379, 692
BstMCI CGRYCG 1 cut(s) 262
BstNI CCWGG 4 cut(s) 298, 495, 523, 990
BstNSI RCATGY 3 cut(s) 401, 1160, 1304
BstSCI CCNGG 4 cut(s) 296, 493, 521, 988
BstSLI GKGCMC 1 cut(s) 358
BstUI CGCG 2 cut(s) 162, 697
BstV1I GCAGC 5 cut(s) 31, 82, 412, 1003, 1006
BstV2I GAAGAC 2 cut(s) 261, 626
BstX2I RGATCY 1 cut(s) 12
BstYI RGATCY 1 cut(s) 12
BstZ17I GTATAC 1 cut(s) 213
Bsu15I ATCGAT 1 cut(s) 126
BsuRI GGCC 2 cut(s) 722, 1281
BsuTUI ATCGAT 1 cut(s) 126
BtgZI GCGATG 1 cut(s) 1347
BtrI CACGTC 1 cut(s) 938
BtsCI GGATG 4 cut(s) 725, 837, 883, 1310
BtsI GCAGTG 1 cut(s) 280
BtsIMutI CAGTG 3 cut(s) 280, 993, 1272
BveI ACCTGC 1 cut(s) 1212
Cac8I GCNNGC 5 cut(s) 45, 100, 271, 528, 1158
CfoI GCGC 2 cut(s) 182, 1348
ClaI ATCGAT 1 cut(s) 126
CseI GACGC 1 cut(s) 101
Csp6I GTAC 5 cut(s) 442, 559, 658, 699, 1351
CspCI CAANNNNNGTGG 2 cut(s) 712, 747
CviAII CATG 9 cut(s) 31, 398, 1009, 1157, 1220, 1240, 1252, 1283, 1301
CviQI GTAC 5 cut(s) 442, 559, 658, 699, 1351
DdeI CTNAG 5 cut(s) 420, 438, 849, 1023, 1110
DinI GGCGCC 2 cut(s) 181, 1347
DpnI GATC 6 cut(s) 14, 37, 116, 261, 381, 694
DpnII GATC 6 cut(s) 12, 35, 114, 259, 379, 692
EaeI YGGCCR 1 cut(s) 720
Eam1104I CTCTTC 5 cut(s) 75, 234, 292, 706, 765
EarI CTCTTC 5 cut(s) 75, 234, 292, 706, 765
EciI GGCGGA 1 cut(s) 256
Eco130I CCWWGG 1 cut(s) 244
Eco24I GRGCYC 1 cut(s) 304
Eco57I CTGAAG 1 cut(s) 165
Eco88I CYCGRG 1 cut(s) 897
EcoNI CCTNNNNNAGG 1 cut(s) 161
EcoRII CCWGG 4 cut(s) 296, 493, 521, 988
EcoT14I CCWWGG 1 cut(s) 244
EcoT38I GRGCYC 1 cut(s) 304
EgeI GGCGCC 2 cut(s) 181, 1347
EheI GGCGCC 2 cut(s) 181, 1347
ErhI CCWWGG 1 cut(s) 244
FaeI CATG 9 cut(s) 34, 401, 1012, 1160, 1223, 1243, 1255, 1286, 1304
FaqI GGGAC 1 cut(s) 1305
FatI CATG 9 cut(s) 30, 397, 1008, 1156, 1219, 1239, 1251, 1282, 1300
FauI CCCGC 2 cut(s) 52, 112
FauNDI CATATG 1 cut(s) 1086
FblI GTMKAC 2 cut(s) 189, 212
FokI GGATG 4 cut(s) 712, 844, 890, 1297
FriOI GRGCYC 1 cut(s) 304
FspBI CTAG 2 cut(s) 351, 828
GlaI GCGC 2 cut(s) 181, 1347
GsuI CTGGAG 2 cut(s) 516, 1296
HaeII RGCGCY 2 cut(s) 183, 1349
HaeIII GGCC 2 cut(s) 722, 1281
HapII CCGG 1 cut(s) 177
HgaI GACGC 1 cut(s) 101
HhaI GCGC 2 cut(s) 182, 1348
Hin1I GRCGYC 2 cut(s) 180, 1346
Hin1II CATG 9 cut(s) 34, 401, 1012, 1160, 1223, 1243, 1255, 1286, 1304
Hin6I GCGC 2 cut(s) 180, 1346
HinP1I GCGC 2 cut(s) 180, 1346
HincII GTYRAC 3 cut(s) 190, 574, 867
HindII GTYRAC 3 cut(s) 190, 574, 867
HindIII AAGCTT 1 cut(s) 598
HinfI GANTC 7 cut(s) 119, 134, 344, 1029, 1054, 1066, 1126
HpaI GTTAAC 1 cut(s) 574
HpaII CCGG 1 cut(s) 177
HphI GGTGA 4 cut(s) 143, 1124, 1193, 1217
Hpy166II GTNNAC 8 cut(s) 190, 213, 395, 559, 574, 867, 977, 1046
Hpy188I TCNGA 2 cut(s) 421, 1381
Hpy188III TCNNGA 6 cut(s) 74, 326, 614, 752, 875, 899
Hpy8I GTNNAC 8 cut(s) 190, 213, 395, 559, 574, 867, 977, 1046
Hpy99I CGWCG 4 cut(s) 95, 143, 173, 194
HpyAV CCTTC 2 cut(s) 506, 1080
HpyCH4III ACNGT 3 cut(s) 311, 360, 864
HpyCH4IV ACGT 2 cut(s) 937, 1294
HpyCH4V TGCA 5 cut(s) 202, 367, 944, 1090, 1300
HpyF3I CTNAG 5 cut(s) 420, 438, 849, 1023, 1110
HpySE526I ACGT 2 cut(s) 937, 1294
Hsp92I GRCGYC 2 cut(s) 180, 1346
Hsp92II CATG 9 cut(s) 34, 401, 1012, 1160, 1223, 1243, 1255, 1286, 1304
HspAI GCGC 2 cut(s) 180, 1346
KasI GGCGCC 2 cut(s) 179, 1345
KspAI GTTAAC 1 cut(s) 574
Kzo9I GATC 6 cut(s) 12, 35, 114, 259, 379, 692
LguI GCTCTTC 2 cut(s) 75, 292
LmnI GCTCC 4 cut(s) 179, 269, 307, 1040
Lsp1109I GCAGC 5 cut(s) 31, 82, 412, 1003, 1006
LweI GCATC 7 cut(s) 568, 653, 653, 822, 835, 953, 1090
MaeI CTAG 2 cut(s) 351, 828
MaeII ACGT 2 cut(s) 937, 1294
MaeIII GTNAC 1 cut(s) 318
MalI GATC 6 cut(s) 14, 37, 116, 261, 381, 694
MbiI CCGCTC 1 cut(s) 1035
MboI GATC 6 cut(s) 12, 35, 114, 259, 379, 692
MfeI CAATTG 1 cut(s) 624
MflI RGATCY 1 cut(s) 12
MhlI GDGCHC 3 cut(s) 304, 358, 454
MlsI TGGCCA 1 cut(s) 722
MluCI AATT 5 cut(s) 49, 217, 415, 624, 1000
MluNI TGGCCA 1 cut(s) 722
Mly113I GGCGCC 2 cut(s) 180, 1346
MlyI GAGTC 3 cut(s) 113, 1060, 1120
Mox20I TGGCCA 1 cut(s) 722
MscI TGGCCA 1 cut(s) 722
MseI TTAA 3 cut(s) 573, 906, 1392
Msp20I TGGCCA 1 cut(s) 722
MspI CCGG 1 cut(s) 177
MspR9I CCNGG 4 cut(s) 298, 495, 523, 990
MunI CAATTG 1 cut(s) 624
Mva1269I GAATGC 1 cut(s) 1388
MvaI CCWGG 4 cut(s) 298, 495, 523, 990
MvnI CGCG 2 cut(s) 162, 697
NarI GGCGCC 2 cut(s) 180, 1346
NdeI CATATG 1 cut(s) 1086
NdeII GATC 6 cut(s) 12, 35, 114, 259, 379, 692
NlaIII CATG 9 cut(s) 34, 401, 1012, 1160, 1223, 1243, 1255, 1286, 1304
NlaIV GGNNCC 5 cut(s) 14, 175, 181, 303, 1347
NmuCI GTSAC 1 cut(s) 318
NspI RCATGY 3 cut(s) 401, 1160, 1304
PaeI GCATGC 1 cut(s) 1160
PaeR7I CTCGAG 1 cut(s) 897
PaqCI CACCTGC 1 cut(s) 1212
PciI ACATGT 1 cut(s) 397
PciSI GCTCTTC 2 cut(s) 75, 292
PcsI WCGNNNNNNNCGW 3 cut(s) 72, 123, 1070
PctI GAATGC 1 cut(s) 1388
PfeI GAWTC 4 cut(s) 134, 344, 1029, 1054
PflMI CCANNNNNTGG 1 cut(s) 568
Ple19I CGATCG 1 cut(s) 262
PleI GAGTC 3 cut(s) 113, 1060, 1120
PluTI GGCGCC 2 cut(s) 183, 1349
PpsI GAGTC 3 cut(s) 113, 1060, 1120
PscI ACATGT 1 cut(s) 397
Psp6I CCWGG 4 cut(s) 296, 493, 521, 988
PspGI CCWGG 4 cut(s) 296, 493, 521, 988
PspN4I GGNNCC 5 cut(s) 14, 175, 181, 303, 1347
PsuI RGATCY 1 cut(s) 12
PvuI CGATCG 1 cut(s) 262
RsaI GTAC 5 cut(s) 443, 560, 659, 700, 1352
RsaNI GTAC 5 cut(s) 442, 559, 658, 699, 1351
SalI GTCGAC 1 cut(s) 188
SapI GCTCTTC 2 cut(s) 75, 292
SaqAI TTAA 3 cut(s) 573, 906, 1392
Sau3AI GATC 6 cut(s) 12, 35, 114, 259, 379, 692
SchI GAGTC 3 cut(s) 113, 1060, 1120
ScrFI CCNGG 4 cut(s) 298, 495, 523, 990
SduI GDGCHC 3 cut(s) 304, 358, 454
SfaNI GCATC 7 cut(s) 568, 653, 653, 822, 835, 953, 1090
SfoI GGCGCC 2 cut(s) 181, 1347
Sfr274I CTCGAG 1 cut(s) 897
SlaI CTCGAG 1 cut(s) 897
SmlI CTYRAG 3 cut(s) 601, 897, 1354
SmoI CTYRAG 3 cut(s) 601, 897, 1354
SphI GCATGC 1 cut(s) 1160
Sse9I AATT 5 cut(s) 49, 217, 415, 624, 1000
SspDI GGCGCC 2 cut(s) 179, 1345
SspI AATATT 1 cut(s) 586
SspMI CTAG 2 cut(s) 351, 828
StyD4I CCNGG 4 cut(s) 296, 493, 521, 988
StyI CCWWGG 1 cut(s) 244
TaaI ACNGT 3 cut(s) 311, 360, 864
TaiI ACGT 2 cut(s) 940, 1297
TaqI TCGA 6 cut(s) 117, 126, 189, 342, 613, 898
TasI AATT 5 cut(s) 49, 217, 415, 624, 1000
TatI WGTACW 2 cut(s) 441, 657
TauI GCSGC 5 cut(s) 43, 110, 282, 285, 1389
TfiI GAWTC 4 cut(s) 134, 344, 1029, 1054
Tru1I TTAA 3 cut(s) 573, 906, 1392
Tru9I TTAA 3 cut(s) 573, 906, 1392
TscAI CASTG 3 cut(s) 280, 1000, 1279
TseFI GTSAC 1 cut(s) 318
TseI GCWGC 5 cut(s) 19, 95, 425, 1016, 1019
Tsp45I GTSAC 1 cut(s) 318
TspDTI ATGAA 4 cut(s) 525, 894, 1154, 1350
TspGWI ACGGA 2 cut(s) 127, 718
TspRI CASTG 3 cut(s) 280, 1000, 1279
Van91I CCANNNNNTGG 1 cut(s) 568
XagI CCTNNNNNAGG 1 cut(s) 161
XceI RCATGY 3 cut(s) 401, 1160, 1304
XhoI CTCGAG 1 cut(s) 897
XmiI GTMKAC 2 cut(s) 189, 212
XspI CTAG 2 cut(s) 351, 828
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.