pycom13g12150

Region found in RelA / SpoT proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0012431
Physical Location & Seq
Forward (+)
8394196 .. 8396555
2360 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1749 bp
ATGGAGCTTTACCTTCCCCACCCTCACCACCGTCTTCCTCACCAGGACCCAATACCCAGAACCCTCGAGCCTACCGTACGGATCTTCCCTTTCACTCTGACTCTACGGAAATGCAACCAGCGAAGCCGACGGCATGGTTTCTCCAAGACGAGGTCGTTTCTGCTGGAGCAGACCGGTGGGAAGATGGTGGTGGAGCTCGTGGGGGCGTTCAACGAGCTCACGGAGAGGATGGGCATGCTCTCCAGCAGCGGCTCCCACTTGCTCTTCAAGGCTCTCAAGCTCTCCATTCCTTTGCTCCATGCTCTGCCTCTGACCCCAGATGGCCGTTCTTCTCTCTCCAGAGCCCTCTCCATCTCTCTCCTCCTCGCCGACCTTCAGATGGATGCTGAAGTAATTTCAGCTGGGATTCTCCGGCCAGTTGTGGAGGCCCGTTCAATTTCGATTCTCGATGTTAGGAACCAGATTGGTTCTGGCACCGCGCATCTCTTGCACGAAAGCTTGCGGGTTATGAAAGTCCCTTCAAAAGTTGATGTCTTGGATGATGACAGTGCAACTGCATTGAGGAAGTTTTGCTTGACTTACTACGATATGAGGGCTGTGATTTTGGACTTGGTGCTCAGGCTTGATGTGATGCGGCATCTTGATTACATGCCGCAGTATCAGCAGCAGTTGACATCTCTAGAAGTAATGAGGCTTCACGCGCCGTTGGCTCATGCTGTTGGGGCCAACTGGCTGTCATTGGAGCTCGAGGATCTTTCGTTTCAGTACTTATTTCCAAATTCGTACCTTTATGTCGATTCTTGGTTGAGAAGTCATGAGACTGGAAGTAAGCCTCTGGTGGATGTGTACAAGGAACAGTTGTTGCGGTCGTTGAGAGTTGATCCGTTGTTGGCAGACATGGTGGTTGATGTTTCAGTTGATGGGCGGTATAAGAGTCGCTATAGCACAATGAAGAAGCTGTTGAAGGATGGTAGGAAGCCAGAGCAAGTGAATGATGTTTTGGGATTGAGAGTCATATTGGAGCCTAGAGATGGAGAAGATATGGCTGAGGTAGGGGAACAAGCATGTTACAGGACGCGCGAAGTTGTTCGATCTATGTGGAAGGAAATGCCTCACAGGACAAAAGACTACATTGCAAGGCCCAAGAAGAATGGGTACCGGAGTTTACATATGGCAGTTGATGTTAGTGACAGTGGGAGGAGTAGGCCGTTGATGGAAATACAGATAAGGACTAAGGAGATGGACATGTTGGCAGATGCTGGAATGGCATCTCACTCATTGTACAAAAGCGGTCTTACAGACCCAGAAGAGGCAAAGAGGCTTAAGGCCATCATGATGGCTGCTGCCGAATTTGCTGCGTTGCGGCTTAAAGATATTAATCACGAAGGCATTGAGACTGAGCAGAGTCAGAATAATCGAGCATTCCGCCTTCTTGACAAGAATGGAGATGGTAGGATCAATATTGAGGAACTTATGGAGGTGATGGAAGAGCTTGGTGCCCCAGGGGAAGATGCATGTGAGATGATGCAGCTACTTGATGCAAATAGTGATGGTTCACTTAGCTCGGATGAATTTGAGTTGTTTCAGAAACAGGTTGGCTTTATGCGTAATTTCGAGGAAAGAGATGAGCAGTACAAGACCGTGTTGAATGAGAAACTTCAAATTGCAGACAGTAGCAGTTTGATCCAGGTGTCTGGTGAAGATCTTAGCAGCAGGGCTTCGGAATTAGATTTGCTTGCATCCGGTTGA

Protein Analysis

583

Amino Acids

65.96

Weight (kDa)

5.85

Isoelectric Point (pI)

43.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HD_4 PF13328 99 - 241 1.2e-20 HD domain
RelA_SpoT PF04607 308 - 431 6.2e-32 Region found in RelA / SpoT proteins
EF-hand_7 PF13499 472 - 526 5.6e-11 EF-hand domain pair
EF-hand_1 PF00036 473 - 497 6.3e-07 EF hand domain
EF-hand_6 PF13405 473 - 499 8.1e-06 EF-hand domain
EF-hand_5 PF13202 474 - 495 4.3e-06 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012431)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17470 AT3G17470 AT3G17470
fragaria_vesca FvH4_4g21520 FvH4_4g21520 FvH4_4g21520
malus_domestica MD13G1139600.v1.1
prunus_persica Prupe.1G105100_v2.0.a1
pyrus_communis pycom13g12150
rosa_chinensis RchiOBHm_Chr4g0427541
rosa_laevigata RLG00000007228
rosa_multiflora Rmu_sc0009627.1_g000004
rosa_roxburghii Rroxscaffold_5G00369190
rosa_rugosa Rorug04G0218400
rosa_samantha Rh4AG275000 Rh4BG280800 Rh4CG296100 Rh4DG278200
rosa_wichuraiana Rw4G023840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1155
AccB1I GGYRCC 3 cut(s) 473, 1155, 1496
AccII CGCG 4 cut(s) 479, 701, 1078, 1080
AclWI GGATC 5 cut(s) 89, 759, 875, 1463, 1678
AcoI YGGCCR 2 cut(s) 322, 413
AcsI RAATTY 3 cut(s) 778, 1349, 1571
AcuI CTGAAG 2 cut(s) 359, 408
AfaI GTAC 7 cut(s) 78, 767, 785, 848, 1157, 1283, 1634
AfiI CCNNNNNNNGG 4 cut(s) 150, 379, 1031, 1309
AflII CTTAAG 1 cut(s) 1322
AflIII ACRYGT 1 cut(s) 1245
AgeI ACCGGT 1 cut(s) 173
AgsI TTSAA 7 cut(s) 211, 268, 435, 522, 964, 1648, 1661
AjnI CCWGG 3 cut(s) 42, 1501, 1686
AjuI GAANNNNNNNTTGG 2 cut(s) 983, 1015
Alw21I GWGCWC 4 cut(s) 198, 219, 618, 747
Alw26I GTCTC 2 cut(s) 812, 1388
AlwI GGATC 5 cut(s) 89, 759, 875, 1463, 1678
AlwNI CAGNNNCTG 1 cut(s) 1259
Ama87I CYCGRG 2 cut(s) 65, 746
AoxI GGCC 7 cut(s) 322, 413, 426, 723, 1139, 1205, 1326
ApeKI GCWGC 7 cut(s) 246, 664, 1340, 1343, 1355, 1528, 1710
ApoI RAATTY 3 cut(s) 778, 1349, 1571
AseI ATTAAT 1 cut(s) 1377
AsiGI ACCGGT 1 cut(s) 173
Asp700I GAANNNNTTC 1 cut(s) 1086
Asp718I GGTACC 1 cut(s) 1155
AspLEI GCGC 3 cut(s) 481, 703, 1080
AspS9I GGNCC 4 cut(s) 46, 427, 723, 1140
AsuHPI GGTGA 4 cut(s) 17, 32, 1492, 1709
AvaI CYCGRG 2 cut(s) 65, 746
AvaII GGWCC 1 cut(s) 46
BaeGI GKGCMC 1 cut(s) 1501
BaeI ACNNNNGTAYC 2 cut(s) 1147, 1180
BanI GGYRCC 3 cut(s) 473, 1155, 1496
BanII GRGCYC 4 cut(s) 198, 219, 346, 747
BarI GAAGNNNNNNTAC 4 cut(s) 678, 710, 1139, 1171
BauI CACGAG 1 cut(s) 197
BbsI GAAGAC 1 cut(s) 26
Bbv12I GWGCWC 4 cut(s) 198, 219, 618, 747
BbvCI CCTCAGC 1 cut(s) 1047
BbvI GCAGC 7 cut(s) 258, 676, 1327, 1330, 1342, 1540, 1722
BceAI ACGGC 4 cut(s) 146, 309, 688, 1192
BcgI CGANNNNNNTGC 2 cut(s) 1337, 1371
BciT130I CCWGG 3 cut(s) 44, 1503, 1688
BcoDI GTCTC 2 cut(s) 812, 1388
BfaI CTAG 2 cut(s) 680, 1026
BfmI CTRYAG 1 cut(s) 940
BfrI CTTAAG 1 cut(s) 1322
BglII AGATCT 1 cut(s) 1702
BmcAI AGTACT 1 cut(s) 767
Bme1390I CCNGG 3 cut(s) 44, 1503, 1688
Bme18I GGWCC 1 cut(s) 46
BmeT110I CYCGRG 2 cut(s) 65, 746
BmgT120I GGNCC 4 cut(s) 46, 427, 723, 1140
BmiI GGNNCC 8 cut(s) 48, 253, 458, 475, 724, 1023, 1157, 1498
BmrFI CCNGG 3 cut(s) 44, 1503, 1688
BmsI GCATC 9 cut(s) 373, 490, 621, 646, 1246, 1277, 1501, 1515, 1528
BpiI GAAGAC 1 cut(s) 26
BpmI CTGGAG 3 cut(s) 185, 226, 322
Bpu10I CCTNAGC 2 cut(s) 617, 1047
BpuEI CTTGAG 1 cut(s) 260
BsaBI GATNNNNATC 1 cut(s) 1377
BsaJI CCNNGG 2 cut(s) 1501, 1502
BsaWI WCCGGW 3 cut(s) 173, 1158, 1742
Bsc4I CCNNNNNNNGG 4 cut(s) 150, 379, 1031, 1309
Bse118I RCCGGY 1 cut(s) 173
Bse1I ACTGG 3 cut(s) 416, 734, 826
Bse3DI GCAATG 1 cut(s) 1131
Bse8I GATNNNNATC 1 cut(s) 1377
BseBI CCWGG 3 cut(s) 44, 1503, 1688
BseDI CCNNGG 2 cut(s) 1501, 1502
BseGI GGATG 7 cut(s) 234, 388, 544, 847, 973, 1573, 1739
BseJI GATNNNNATC 1 cut(s) 1377
BseLI CCNNNNNNNGG 4 cut(s) 150, 379, 1031, 1309
BseMI GCAATG 1 cut(s) 1131
BseMII CTCAG 3 cut(s) 631, 1038, 1389
BseNI ACTGG 3 cut(s) 416, 734, 826
BseRI GAGGAG 3 cut(s) 350, 353, 1213
BseSI GKGCMC 1 cut(s) 1501
BseXI GCAGC 7 cut(s) 258, 676, 1327, 1330, 1342, 1540, 1722
BseYI CCCAGC 1 cut(s) 401
Bsh1236I CGCG 4 cut(s) 479, 701, 1078, 1080
Bsh1285I CGRYCG 1 cut(s) 869
BshFI GGCC 7 cut(s) 324, 415, 428, 725, 1141, 1207, 1328
BshNI GGYRCC 3 cut(s) 473, 1155, 1496
BshTI ACCGGT 1 cut(s) 173
BsiEI CGRYCG 1 cut(s) 869
BsiHKAI GWGCWC 4 cut(s) 198, 219, 618, 747
BsiHKCI CYCGRG 2 cut(s) 65, 746
BsiSI CCGG 4 cut(s) 174, 412, 1159, 1743
BsiWI CGTACG 1 cut(s) 76
BslFI GGGAC 1 cut(s) 500
BslI CCNNNNNNNGG 4 cut(s) 150, 379, 1031, 1309
BsmAI GTCTC 2 cut(s) 812, 1388
BsmFI GGGAC 1 cut(s) 500
BsmI GAATGC 1 cut(s) 1421
BsnI GGCC 7 cut(s) 324, 415, 428, 725, 1141, 1207, 1328
BsoBI CYCGRG 2 cut(s) 65, 746
Bsp1286I GDGCHC 6 cut(s) 198, 219, 346, 618, 747, 1501
Bsp1407I TGTACA 2 cut(s) 846, 1281
Bsp143I GATC 7 cut(s) 81, 751, 880, 1091, 1455, 1683, 1702
BspANI GGCC 7 cut(s) 324, 415, 428, 725, 1141, 1207, 1328
BspCNI CTCAG 3 cut(s) 630, 1039, 1390
BspFNI CGCG 4 cut(s) 479, 701, 1078, 1080
BspHI TCATGA 2 cut(s) 814, 1332
BspLI GGNNCC 8 cut(s) 48, 253, 458, 475, 724, 1023, 1157, 1498
BspPI GGATC 5 cut(s) 89, 759, 875, 1463, 1678
BspQI GCTCTTC 2 cut(s) 269, 1482
BspT107I GGYRCC 3 cut(s) 473, 1155, 1496
BspTI CTTAAG 1 cut(s) 1322
BsrDI GCAATG 1 cut(s) 1131
BsrFI RCCGGY 1 cut(s) 173
BsrGI TGTACA 2 cut(s) 846, 1281
BsrI ACTGG 3 cut(s) 416, 734, 826
BssAI RCCGGY 1 cut(s) 173
BssECI CCNNGG 2 cut(s) 1501, 1502
BssMI GATC 7 cut(s) 81, 751, 880, 1091, 1455, 1683, 1702
BssSI CACGAG 1 cut(s) 197
Bst2BI CACGAG 1 cut(s) 197
Bst2UI CCWGG 3 cut(s) 44, 1503, 1688
Bst4CI ACNGT 7 cut(s) 32, 76, 548, 858, 1193, 1642, 1673
Bst6I CTCTTC 3 cut(s) 269, 1302, 1482
BstAFI CTTAAG 1 cut(s) 1322
BstAPI GCANNNNNTGC 1 cut(s) 487
BstAUI TGTACA 2 cut(s) 846, 1281
BstC8I GCNNGC 3 cut(s) 236, 500, 1737
BstDEI CTNAG 6 cut(s) 617, 1047, 1233, 1398, 1559, 1706
BstF5I GGATG 7 cut(s) 234, 388, 544, 847, 973, 1573, 1739
BstFNI CGCG 4 cut(s) 479, 701, 1078, 1080
BstHHI GCGC 3 cut(s) 481, 703, 1080
BstKTI GATC 7 cut(s) 84, 754, 883, 1094, 1458, 1686, 1705
BstMAI GTCTC 2 cut(s) 812, 1388
BstMBI GATC 7 cut(s) 81, 751, 880, 1091, 1455, 1683, 1702
BstMCI CGRYCG 1 cut(s) 869
BstMWI GCNNNNNNNGC 7 cut(s) 487, 661, 700, 707, 722, 1265, 1352
BstNI CCWGG 3 cut(s) 44, 1503, 1688
BstNSI RCATGY 5 cut(s) 238, 652, 1068, 1249, 1518
BstSCI CCNGG 3 cut(s) 42, 1501, 1686
BstSFI CTRYAG 1 cut(s) 940
BstSLI GKGCMC 1 cut(s) 1501
BstUI CGCG 4 cut(s) 479, 701, 1078, 1080
BstV1I GCAGC 7 cut(s) 258, 676, 1327, 1330, 1342, 1540, 1722
BstV2I GAAGAC 1 cut(s) 26
BstX2I RGATCY 3 cut(s) 81, 751, 1702
BstXI CCANNNNNNTGG 2 cut(s) 1336, 1694
BstYI RGATCY 3 cut(s) 81, 751, 1702
BsuRI GGCC 7 cut(s) 324, 415, 428, 725, 1141, 1207, 1328
BtsCI GGATG 7 cut(s) 234, 388, 544, 847, 973, 1573, 1739
BtsIMutI CAGTG 2 cut(s) 553, 1198
Cac8I GCNNGC 3 cut(s) 236, 500, 1737
CaiI CAGNNNCTG 1 cut(s) 1259
CciI TCATGA 2 cut(s) 814, 1332
CfoI GCGC 3 cut(s) 481, 703, 1080
Cfr10I RCCGGY 1 cut(s) 173
Cfr13I GGNCC 4 cut(s) 46, 427, 723, 1140
CseI GACGC 1 cut(s) 1084
Csp6I GTAC 7 cut(s) 77, 766, 784, 847, 1156, 1282, 1633
CspAI ACCGGT 1 cut(s) 173
CviQI GTAC 7 cut(s) 77, 766, 784, 847, 1156, 1282, 1633
DdeI CTNAG 6 cut(s) 617, 1047, 1233, 1398, 1559, 1706
DpnI GATC 7 cut(s) 83, 753, 882, 1093, 1457, 1685, 1704
DpnII GATC 7 cut(s) 81, 751, 880, 1091, 1455, 1683, 1702
EaeI YGGCCR 2 cut(s) 322, 413
Eam1104I CTCTTC 3 cut(s) 269, 1302, 1482
EarI CTCTTC 3 cut(s) 269, 1302, 1482
EciI GGCGGA 1 cut(s) 1415
Ecl136II GAGCTC 3 cut(s) 196, 217, 745
Eco24I GRGCYC 4 cut(s) 198, 219, 346, 747
Eco47I GGWCC 1 cut(s) 46
Eco53kI GAGCTC 3 cut(s) 196, 217, 745
Eco57I CTGAAG 2 cut(s) 359, 408
Eco88I CYCGRG 2 cut(s) 65, 746
EcoICRI GAGCTC 3 cut(s) 196, 217, 745
EcoO109I RGGNCCY 1 cut(s) 46
EcoRII CCWGG 3 cut(s) 42, 1501, 1686
EcoT22I ATGCAT 1 cut(s) 1516
EcoT38I GRGCYC 4 cut(s) 198, 219, 346, 747
FalI AAGNNNNNCTT 2 cut(s) 557, 589
FaqI GGGAC 1 cut(s) 500
FauI CCCGC 1 cut(s) 495
FauNDI CATATG 1 cut(s) 1170
FokI GGATG 7 cut(s) 241, 395, 551, 854, 980, 1580, 1726
FriOI GRGCYC 4 cut(s) 198, 219, 346, 747
FspBI CTAG 2 cut(s) 680, 1026
GlaI GCGC 3 cut(s) 480, 702, 1079
GsaI CCCAGC 1 cut(s) 405
GsuI CTGGAG 3 cut(s) 185, 226, 322
HaeIII GGCC 7 cut(s) 324, 415, 428, 725, 1141, 1207, 1328
HapII CCGG 4 cut(s) 174, 412, 1159, 1743
HgaI GACGC 1 cut(s) 1084
HhaI GCGC 3 cut(s) 481, 703, 1080
Hin6I GCGC 3 cut(s) 479, 701, 1078
HinP1I GCGC 3 cut(s) 479, 701, 1078
HincII GTYRAC 1 cut(s) 672
HindII GTYRAC 1 cut(s) 672
HindIII AAGCTT 1 cut(s) 496
HinfI GANTC 7 cut(s) 100, 406, 442, 797, 934, 1011, 1405
HpaII CCGG 4 cut(s) 174, 412, 1159, 1743
HphI GGTGA 4 cut(s) 17, 32, 1492, 1709
Hpy166II GTNNAC 4 cut(s) 672, 847, 1166, 1556
Hpy188I TCNGA 7 cut(s) 99, 312, 378, 1410, 1567, 1587, 1723
Hpy188III TCNNGA 8 cut(s) 339, 446, 641, 680, 815, 1333, 1382, 1433
Hpy8I GTNNAC 4 cut(s) 672, 847, 1166, 1556
Hpy99I CGWCG 1 cut(s) 132
HpyAV CCTTC 7 cut(s) 23, 383, 528, 958, 1096, 1379, 1439
HpyCH4III ACNGT 7 cut(s) 32, 76, 548, 858, 1193, 1642, 1673
HpyF10VI GCNNNNNNNGC 7 cut(s) 487, 661, 700, 707, 722, 1265, 1352
HpyF3I CTNAG 6 cut(s) 617, 1047, 1233, 1398, 1559, 1706
HspAI GCGC 3 cut(s) 479, 701, 1078
KpnI GGTACC 1 cut(s) 1159
Kzo9I GATC 7 cut(s) 81, 751, 880, 1091, 1455, 1683, 1702
LguI GCTCTTC 2 cut(s) 269, 1482
LmnI GCTCC 7 cut(s) 4, 166, 193, 257, 300, 742, 1021
Lsp1109I GCAGC 7 cut(s) 258, 676, 1327, 1330, 1342, 1540, 1722
LweI GCATC 9 cut(s) 373, 490, 621, 646, 1246, 1277, 1501, 1515, 1528
MaeI CTAG 2 cut(s) 680, 1026
MaeIII GTNAC 2 cut(s) 1067, 1187
MalI GATC 7 cut(s) 83, 753, 882, 1093, 1457, 1685, 1704
MboI GATC 7 cut(s) 81, 751, 880, 1091, 1455, 1683, 1702
MflI RGATCY 3 cut(s) 81, 751, 1702
MhlI GDGCHC 6 cut(s) 198, 219, 346, 618, 747, 1501
MluCI AATT 8 cut(s) 393, 435, 778, 1349, 1571, 1609, 1662, 1724
MlyI GAGTC 4 cut(s) 94, 943, 1020, 1414
Mph1103I ATGCAT 1 cut(s) 1516
MroXI GAANNNNTTC 1 cut(s) 1086
MseI TTAA 3 cut(s) 1323, 1368, 1377
MslI CAYNNNNRTG 1 cut(s) 1334
MspA1I CMGCKG 2 cut(s) 249, 401
MspCI CTTAAG 1 cut(s) 1322
MspI CCGG 4 cut(s) 174, 412, 1159, 1743
MspR9I CCNGG 3 cut(s) 44, 1503, 1688
Mva1269I GAATGC 1 cut(s) 1421
MvaI CCWGG 3 cut(s) 44, 1503, 1688
MvnI CGCG 4 cut(s) 479, 701, 1078, 1080
MwoI GCNNNNNNNGC 7 cut(s) 487, 661, 700, 707, 722, 1265, 1352
NdeI CATATG 1 cut(s) 1170
NdeII GATC 7 cut(s) 81, 751, 880, 1091, 1455, 1683, 1702
NlaIV GGNNCC 8 cut(s) 48, 253, 458, 475, 724, 1023, 1157, 1498
NmuCI GTSAC 1 cut(s) 1187
NsiI ATGCAT 1 cut(s) 1516
NspI RCATGY 5 cut(s) 238, 652, 1068, 1249, 1518
PaeI GCATGC 1 cut(s) 238
PaeR7I CTCGAG 2 cut(s) 65, 746
PagI TCATGA 2 cut(s) 814, 1332
PasI CCCWGGG 1 cut(s) 1502
PciI ACATGT 1 cut(s) 1245
PciSI GCTCTTC 2 cut(s) 269, 1482
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PctI GAATGC 1 cut(s) 1421
PdmI GAANNNNTTC 1 cut(s) 1086
PfeI GAWTC 3 cut(s) 406, 442, 797
Pfl23II CGTACG 1 cut(s) 76
PflFI GACNNNGTC 1 cut(s) 151
PinAI ACCGGT 1 cut(s) 173
PleI GAGTC 4 cut(s) 94, 942, 1019, 1413
PpsI GAGTC 4 cut(s) 94, 942, 1019, 1413
PpuMI RGGWCCY 1 cut(s) 46
PscI ACATGT 1 cut(s) 1245
PshBI ATTAAT 1 cut(s) 1377
Psp124BI GAGCTC 3 cut(s) 198, 219, 747
Psp5II RGGWCCY 1 cut(s) 46
Psp6I CCWGG 3 cut(s) 42, 1501, 1686
PspFI CCCAGC 1 cut(s) 401
PspGI CCWGG 3 cut(s) 42, 1501, 1686
PspLI CGTACG 1 cut(s) 76
PspN4I GGNNCC 8 cut(s) 48, 253, 458, 475, 724, 1023, 1157, 1498
PspPI GGNCC 4 cut(s) 46, 427, 723, 1140
PspPPI RGGWCCY 1 cut(s) 46
PspXI VCTCGAGB 2 cut(s) 65, 746
PstNI CAGNNNCTG 1 cut(s) 1259
PsuI RGATCY 3 cut(s) 81, 751, 1702
PsyI GACNNNGTC 1 cut(s) 151
PvuII CAGCTG 1 cut(s) 401
RsaI GTAC 7 cut(s) 78, 767, 785, 848, 1157, 1283, 1634
RsaNI GTAC 7 cut(s) 77, 766, 784, 847, 1156, 1282, 1633
RseI CAYNNNNRTG 1 cut(s) 1334
SacI GAGCTC 3 cut(s) 198, 219, 747
SapI GCTCTTC 2 cut(s) 269, 1482
SaqAI TTAA 3 cut(s) 1323, 1368, 1377
Sau3AI GATC 7 cut(s) 81, 751, 880, 1091, 1455, 1683, 1702
Sau96I GGNCC 4 cut(s) 46, 427, 723, 1140
ScaI AGTACT 1 cut(s) 767
SchI GAGTC 4 cut(s) 94, 943, 1020, 1414
ScrFI CCNGG 3 cut(s) 44, 1503, 1688
SduI GDGCHC 6 cut(s) 198, 219, 346, 618, 747, 1501
SfaNI GCATC 9 cut(s) 373, 490, 621, 646, 1246, 1277, 1501, 1515, 1528
SfcI CTRYAG 1 cut(s) 940
Sfr274I CTCGAG 2 cut(s) 65, 746
SinI GGWCC 1 cut(s) 46
SlaI CTCGAG 2 cut(s) 65, 746
SmiMI CAYNNNNRTG 1 cut(s) 1334
SmlI CTYRAG 4 cut(s) 65, 275, 746, 1322
SmoI CTYRAG 4 cut(s) 65, 275, 746, 1322
SphI GCATGC 1 cut(s) 238
Sse9I AATT 8 cut(s) 393, 435, 778, 1349, 1571, 1609, 1662, 1724
SspI AATATT 1 cut(s) 1462
SspMI CTAG 2 cut(s) 680, 1026
SstI GAGCTC 3 cut(s) 198, 219, 747
StyD4I CCNGG 3 cut(s) 42, 1501, 1686
TaaI ACNGT 7 cut(s) 32, 76, 548, 858, 1193, 1642, 1673
TaqI TCGA 8 cut(s) 66, 440, 447, 747, 795, 1090, 1417, 1614
TasI AATT 8 cut(s) 393, 435, 778, 1349, 1571, 1609, 1662, 1724
TatI WGTACW 4 cut(s) 765, 846, 1281, 1632
TauI GCSGC 4 cut(s) 252, 637, 655, 1366
TfiI GAWTC 3 cut(s) 406, 442, 797
Tru1I TTAA 3 cut(s) 1323, 1368, 1377
Tru9I TTAA 3 cut(s) 1323, 1368, 1377
TscAI CASTG 2 cut(s) 553, 1198
TseFI GTSAC 1 cut(s) 1187
TseI GCWGC 7 cut(s) 246, 664, 1340, 1343, 1355, 1528, 1710
Tsp45I GTSAC 1 cut(s) 1187
TspDTI ATGAA 3 cut(s) 524, 965, 1584
TspGWI ACGGA 4 cut(s) 94, 121, 236, 873
TspRI CASTG 2 cut(s) 553, 1198
Tth111I GACNNNGTC 1 cut(s) 151
Vha464I CTTAAG 1 cut(s) 1322
VpaK11BI GGWCC 1 cut(s) 46
VspI ATTAAT 1 cut(s) 1377
XapI RAATTY 3 cut(s) 778, 1349, 1571
XbaI TCTAGA 1 cut(s) 679
XceI RCATGY 5 cut(s) 238, 652, 1068, 1249, 1518
XcmI CCANNNNNNNNNTGG 1 cut(s) 467
XhoI CTCGAG 2 cut(s) 65, 746
XmnI GAANNNNTTC 1 cut(s) 1086
XspI CTAG 2 cut(s) 680, 1026
ZrmI AGTACT 1 cut(s) 767
Zsp2I ATGCAT 1 cut(s) 1516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.