pycom13g13440
ERF Family

Belongs to the small GTPase superfamily. SAR1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0017489
Physical Location & Seq
Forward (+)
9441612 .. 9442848
1237 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 582 bp
ATGTTTCTGGTGGATTGGTTCTATGGAGTTCTGGCTTCTCTAGGGCTATGGCAGAAGGAGGCCAAGATCTTGTTCTTAGGGCTCGATAATGCCGGAAAGACCACCTTGCTTCACATGCTCAAAGATGAGAGATTAGTTCAGCACCAACCGACGCAGCATCCGACGTCGGAGGAGCTGAGTATTGGGATGATTAAGTTCAAGGCCTTTGATTTGGGCGGGCATCAGGTCGCTCGCAGAGTCTGGAAGGACTATTACGCCAAGGTGGATGCTGTGGTGTACCTGGTTGATGCTTACGACAAGGAGAGATTTGCAGAGTCAAAGAAGGAGCTCGATGCTCTTCTCTCCGACGAGTCATTGGCAACTGTCCCGTTTCTGATCTTGGGAAACAAAATCGACATACCTTATGCTGCATCAGAGGATGAGCTACGTTACCATTTGGGCCTGACTAACTTCACCACAGGTAAGGGGAAGGTCAATCTGACCGATTCCAATGTCCGCCCCCTTGAAGTGTTCATGTGCAGTATTGTCCGCAAGATGGGGTATGGTGAAGGCTTCAAATGGCTGTCCCAATACATCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 ko05134 map04141 map05134
Pfam Domains
Protein Families
Arf

Protein Analysis

194

Amino Acids

22.03

Weight (kDa)

6.52

Isoelectric Point (pI)

25.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 8 - 192 6.1e-65 ADP-ribosylation factor family
SRPRB PF09439 19 - 144 6.3e-08 Signal recognition particle receptor beta subunit
Roc PF08477 22 - 132 5.3e-08 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 22 - 130 3e-07 50S ribosome-binding GTPase
Ras PF00071 22 - 134 1.5e-06 Ras family
G-alpha PF00503 49 - 133 1.8e-08 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017489)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02080
fragaria_vesca FvH4_4g19410
malus_domestica MD16G1157800.v1.1
pyrus_communis pycom13g13440
rosa_chinensis RchiOBHm_Chr4g0424441
rosa_laevigata RLG00000007474
rosa_multiflora Rmu_sc0003567.1_g000010
rosa_roxburghii Rroxscaffold_5G00366380
rosa_samantha Rh4AG253900 Rh4BG259200 Rh4CG270500 Rh4DG253700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 167
AciI CCGC 3 cut(s) 216, 496, 529
AcyI GRCGYC 1 cut(s) 164
AfaI GTAC 1 cut(s) 278
AfiI CCNNNNNNNGG 1 cut(s) 535
AgsI TTSAA 3 cut(s) 199, 506, 556
AjnI CCWGG 1 cut(s) 279
AjuI GAANNNNNNNTTGG 2 cut(s) 56, 88
AluBI AGCT 3 cut(s) 175, 328, 424
AluI AGCT 3 cut(s) 175, 328, 424
Alw21I GWGCWC 1 cut(s) 330
AlwNI CAGNNNCTG 1 cut(s) 240
AoxI GGCC 3 cut(s) 60, 201, 439
ApeKI GCWGC 2 cut(s) 154, 407
AspS9I GGNCC 1 cut(s) 439
AsuHPI GGTGA 2 cut(s) 445, 557
BanII GRGCYC 2 cut(s) 84, 330
BarI GAAGNNNNNNTAC 2 cut(s) 236, 268
Bbv12I GWGCWC 1 cut(s) 330
BbvI GCAGC 2 cut(s) 166, 394
BccI CCATC 1 cut(s) 529
BciT130I CCWGG 1 cut(s) 281
BfaI CTAG 1 cut(s) 41
BglII AGATCT 1 cut(s) 66
BisI GCNGC 2 cut(s) 155, 408
BlsI GCNGC 2 cut(s) 156, 409
Bme1390I CCNGG 1 cut(s) 281
BmgT120I GGNCC 1 cut(s) 439
BmrFI CCNGG 1 cut(s) 281
BmsI GCATC 6 cut(s) 166, 229, 256, 277, 322, 419
BsaHI GRCGYC 1 cut(s) 164
BsaJI CCNNGG 1 cut(s) 258
Bsc4I CCNNNNNNNGG 1 cut(s) 535
BseBI CCWGG 1 cut(s) 281
BseDI CCNNGG 1 cut(s) 258
BseGI GGATG 4 cut(s) 157, 192, 271, 424
BseLI CCNNNNNNNGG 1 cut(s) 535
BseMII CTCAG 1 cut(s) 167
BseRI GAGGAG 1 cut(s) 185
BseXI GCAGC 2 cut(s) 166, 394
BsgI GTGCAG 1 cut(s) 538
BshFI GGCC 3 cut(s) 62, 203, 441
BsiHKAI GWGCWC 1 cut(s) 330
BsiSI CCGG 1 cut(s) 93
BslFI GGGAC 2 cut(s) 350, 550
BslI CCNNNNNNNGG 1 cut(s) 535
BsmFI GGGAC 2 cut(s) 350, 550
BsnI GGCC 3 cut(s) 62, 203, 441
Bsp1286I GDGCHC 2 cut(s) 84, 330
Bsp143I GATC 2 cut(s) 66, 375
BspACI CCGC 3 cut(s) 216, 496, 529
BspANI GGCC 3 cut(s) 62, 203, 441
BspCNI CTCAG 1 cut(s) 168
BspQI GCTCTTC 1 cut(s) 342
BssECI CCNNGG 1 cut(s) 258
BssMI GATC 2 cut(s) 66, 375
BssNI GRCGYC 1 cut(s) 164
BssT1I CCWWGG 1 cut(s) 258
Bst2UI CCWGG 1 cut(s) 281
Bst4CI ACNGT 1 cut(s) 364
Bst6I CTCTTC 1 cut(s) 342
BstACI GRCGYC 1 cut(s) 164
BstC8I GCNNGC 2 cut(s) 218, 232
BstDEI CTNAG 2 cut(s) 76, 176
BstF5I GGATG 4 cut(s) 157, 192, 271, 424
BstKTI GATC 2 cut(s) 69, 378
BstMBI GATC 2 cut(s) 66, 375
BstMWI GCNNNNNNNGC 1 cut(s) 115
BstNI CCWGG 1 cut(s) 281
BstNSI RCATGY 1 cut(s) 118
BstSCI CCNGG 1 cut(s) 279
BstV1I GCAGC 2 cut(s) 166, 394
BstX2I RGATCY 1 cut(s) 66
BstYI RGATCY 1 cut(s) 66
BsuRI GGCC 3 cut(s) 62, 203, 441
BtsCI GGATG 4 cut(s) 157, 192, 271, 424
Cac8I GCNNGC 2 cut(s) 218, 232
CaiI CAGNNNCTG 1 cut(s) 240
Cfr13I GGNCC 1 cut(s) 439
CseI GACGC 1 cut(s) 160
CsiI ACCWGGT 1 cut(s) 279
Csp6I GTAC 1 cut(s) 277
CviAII CATG 2 cut(s) 115, 514
CviQI GTAC 1 cut(s) 277
DdeI CTNAG 2 cut(s) 76, 176
DpnI GATC 2 cut(s) 68, 377
DpnII GATC 2 cut(s) 66, 375
Eam1104I CTCTTC 1 cut(s) 342
EarI CTCTTC 1 cut(s) 342
EciI GGCGGA 1 cut(s) 485
Ecl136II GAGCTC 1 cut(s) 328
Eco130I CCWWGG 1 cut(s) 258
Eco147I AGGCCT 1 cut(s) 203
Eco24I GRGCYC 2 cut(s) 84, 330
Eco53kI GAGCTC 1 cut(s) 328
EcoICRI GAGCTC 1 cut(s) 328
EcoRII CCWGG 1 cut(s) 279
EcoT14I CCWWGG 1 cut(s) 258
EcoT38I GRGCYC 2 cut(s) 84, 330
ErhI CCWWGG 1 cut(s) 258
FaeI CATG 2 cut(s) 118, 517
FaiI YATR 7 cut(s) 24, 49, 116, 398, 405, 515, 543
FalI AAGNNNNNCTT 2 cut(s) 89, 121
FaqI GGGAC 2 cut(s) 350, 550
FatI CATG 2 cut(s) 114, 513
FauI CCCGC 1 cut(s) 209
Fnu4HI GCNGC 2 cut(s) 155, 408
FokI GGATG 4 cut(s) 144, 199, 278, 431
FriOI GRGCYC 2 cut(s) 84, 330
Fsp4HI GCNGC 2 cut(s) 155, 408
FspBI CTAG 1 cut(s) 41
GluI GCNGC 2 cut(s) 155, 408
HaeIII GGCC 3 cut(s) 62, 203, 441
HapII CCGG 1 cut(s) 93
HgaI GACGC 1 cut(s) 160
Hin1I GRCGYC 1 cut(s) 164
Hin1II CATG 2 cut(s) 118, 517
HinfI GANTC 4 cut(s) 237, 314, 350, 485
HpaII CCGG 1 cut(s) 93
HphI GGTGA 2 cut(s) 445, 557
Hpy166II GTNNAC 1 cut(s) 277
Hpy188I TCNGA 6 cut(s) 162, 169, 346, 375, 415, 480
Hpy188III TCNNGA 1 cut(s) 241
Hpy8I GTNNAC 1 cut(s) 277
Hpy99I CGWCG 4 cut(s) 154, 166, 169, 350
HpyAV CCTTC 5 cut(s) 49, 238, 316, 463, 542
HpyCH4III ACNGT 1 cut(s) 364
HpyCH4IV ACGT 2 cut(s) 164, 427
HpyCH4V TGCA 3 cut(s) 311, 410, 519
HpyF10VI GCNNNNNNNGC 1 cut(s) 115
HpyF3I CTNAG 2 cut(s) 76, 176
HpySE526I ACGT 2 cut(s) 164, 427
Hsp92I GRCGYC 1 cut(s) 164
Hsp92II CATG 2 cut(s) 118, 517
Kzo9I GATC 2 cut(s) 66, 375
LguI GCTCTTC 1 cut(s) 342
LmnI GCTCC 2 cut(s) 172, 325
LpnPI CCDG 8 cut(s) 17, 106, 209, 226, 266, 293, 444, 455
Lsp1109I GCAGC 2 cut(s) 166, 394
LweI GCATC 6 cut(s) 166, 229, 256, 277, 322, 419
MabI ACCWGGT 1 cut(s) 279
MaeI CTAG 1 cut(s) 41
MaeII ACGT 2 cut(s) 164, 427
MaeIII GTNAC 1 cut(s) 428
MalI GATC 2 cut(s) 68, 377
MboI GATC 2 cut(s) 66, 375
MboII GAAGA 1 cut(s) 329
MflI RGATCY 1 cut(s) 66
MhlI GDGCHC 2 cut(s) 84, 330
MlyI GAGTC 3 cut(s) 246, 323, 359
MmeI TCCRAC 3 cut(s) 147, 185, 369
MnlI CCTC 3 cut(s) 52, 163, 409
MseI TTAA 1 cut(s) 192
MspI CCGG 1 cut(s) 93
MspR9I CCNGG 1 cut(s) 281
MvaI CCWGG 1 cut(s) 281
MwoI GCNNNNNNNGC 1 cut(s) 115
NdeII GATC 2 cut(s) 66, 375
NlaIII CATG 2 cut(s) 118, 517
NspI RCATGY 1 cut(s) 118
PceI AGGCCT 1 cut(s) 203
PciSI GCTCTTC 1 cut(s) 342
PcsI WCGNNNNNNNCGW 1 cut(s) 158
PfeI GAWTC 1 cut(s) 485
PkrI GCNGC 2 cut(s) 156, 409
PleI GAGTC 3 cut(s) 245, 322, 358
PpsI GAGTC 3 cut(s) 245, 322, 358
Psp124BI GAGCTC 1 cut(s) 330
Psp6I CCWGG 1 cut(s) 279
PspGI CCWGG 1 cut(s) 279
PspPI GGNCC 1 cut(s) 439
PstNI CAGNNNCTG 1 cut(s) 240
PsuI RGATCY 1 cut(s) 66
RsaI GTAC 1 cut(s) 278
RsaNI GTAC 1 cut(s) 277
SacI GAGCTC 1 cut(s) 330
SapI GCTCTTC 1 cut(s) 342
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 2 cut(s) 155, 408
Sau3AI GATC 2 cut(s) 66, 375
Sau96I GGNCC 1 cut(s) 439
SchI GAGTC 3 cut(s) 246, 323, 359
ScrFI CCNGG 1 cut(s) 281
SduI GDGCHC 2 cut(s) 84, 330
SexAI ACCWGGT 1 cut(s) 279
SfaNI GCATC 6 cut(s) 166, 229, 256, 277, 322, 419
SseBI AGGCCT 1 cut(s) 203
SsiI CCGC 3 cut(s) 216, 496, 529
SspMI CTAG 1 cut(s) 41
SstI GAGCTC 1 cut(s) 330
StuI AGGCCT 1 cut(s) 203
StyD4I CCNGG 1 cut(s) 279
StyI CCWWGG 1 cut(s) 258
TaaI ACNGT 1 cut(s) 364
TaiI ACGT 2 cut(s) 167, 430
TaqI TCGA 3 cut(s) 84, 330, 393
TaqII GACCGA 1 cut(s) 497
TfiI GAWTC 1 cut(s) 485
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TseI GCWGC 2 cut(s) 154, 407
TspDTI ATGAA 1 cut(s) 502
XceI RCATGY 1 cut(s) 118
XspI CTAG 1 cut(s) 41
ZraI GACGTC 1 cut(s) 165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.