pycom13g13820

Topless-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0017337
Physical Location & Seq
Reverse (-)
9818797 .. 9822418
3622 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1677 bp
ATGACTGCTTGTTCTAATGCATTTCAGGTTGCTATGGTCAAAGATACGTCGATGTCTGTAAGCCGTGTATCATGGAATCAAGATGGAAGCTTAATTGGTGTTGCATTTACCAAGCACTTGGTTCATTTGTACTCTTATCAAGGACCAACTGATCTACGGCAACATTTGGAGATTGATGCTCACAGTGGCAGTGTGAATGACTTAGCCTTTTCTCATCCAAACAAGCAATTATGTATTATAACTTGTGGGGAGGATAAGCTGATAAAGGTTTGGGATTTGGTTGGAAGAAATCTATACCGCCTTGAAGGTCATGAAGCGCCTGTTTACTCAATTTGCCCCCACCAGAAGGAGAATATTCAGTTCGTATTTTCAACTGCTGTTGATGGGAGCATAACAGCTTGGCTGTATGACAATGTGGGCTCCAGGGTTGACTATAATGCTCCTGGGCAGTGGTGCACCACAATTCTCTACAGTGATGACGGAAATAGATTATTCTCTTGTGGGACTAGTAAAGATGGTGATTCTTTCCTCGTTGAATGGAATGAAAGTGAAAGGGCAATAAAGAGGACATATAATGGATTCAGAAAAAAATCTTCTGGCATAGTGCAATTTGATACAACAAAAAATCTGTTTTTGGCTGTTGGTGAAGATAATCAGATAAAGTTTTGGGATATGGATAATACAAATGTTCTTACTAGTACAGATGCTGAGGGTGGACTTCTGACTCTTCCCCGCTTGAGATTCAATAAGGAAGGAAATTTGCTTGCTGCTACCACGTCTGACAACGGAATCAAAATTCTTGCTAATGCTGAGGGTCTAAGATCCTTAAGAGCAATTGAAACTCGATCTTATGAAGCATCCAGAGGACGTATTGAGATGAAGGTATCTGGTTCTTCCATGGTTCCGAATGTCAACCCAACCATTAATAAAGTGAACCGTATGGATACAAGCTCTCCTGCCATACCGACTCATATTCTTAATGGAGCCGATTCTATGGCAAGAAGCCTGGAAAAAAGAAGCTTAGATGATGTATCTGACAGGATTAAACGTTGGGAACTGTCGGAGATTGTAGATCCTGTTCAGTGTCGAGTGGCTACTATGCCAGAAAGTAAAGATCCTGCTAAAAAGGTTGCTCGACTTCTGTACACAAATTCTGGTTCTGGCATTCTGGCTCTCGGTTCAAACGGGGTGCAGAAGCTATGGAAGTGGAGCCGCAATGAACAAAATCCAAGTGGGAAGGCCACAGCAAGTGTTGTTCCCCAACATTGGCAACCCAACAGTGGTCTTTTAATGACTAATGATGTCTCAGAAAATGTTGAAGAAGCAGTTCCATGCATTGCACTCTCAAAAAATGACTCTTATGTGATGTCAGCCTGTGGAGGAAAAGTGTCTCTGTTCAATATGATGACTTTTAAGGTAATGACAACTTTCATGCCACCTCCACCAGTGTCAACCTACTTGTCATTTCATCCTCTGGATAATAATATCATAGCAATAGGAATGGAAGATTCAACTATCCACATTTACAATGTTCGACTTGATGAGGTCAAAGCAAAACTGAAAGGCCACCAGAAGCAAATTACTGGTTTAGCATTTTCTGTCAACCTTAAAATCATGGTTTCATCAGGTGCCGATGCTCAAGTATCTATGCTTTTGGAACTTGGATACATGGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

559

Amino Acids

61.6

Weight (kDa)

6.65

Isoelectric Point (pI)

38.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 9 - 134 1.6e-13 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 11 - 93 1e-06 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 15 - 231 5e-19 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 18 - 272 1e-22 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 29 - 137 1e-07 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 53 - 134 3.8e-06 WDR3 second beta-propeller domain
WD40 PF00400 57 - 92 3.2e-06 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 72 - 205 3.2e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 74 - 282 5.6e-11 Prp19 WD40 domain
WDR55 PF24796 105 - 266 5.6e-07 WDR55
Beta-prop_WDR3_1st PF25173 125 - 266 1.5e-07 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 374 - 549 9.5e-14 Prp19 WD40 domain
WDR55 PF24796 434 - 549 5.1e-07 WDR55
Beta-prop_WDR3_1st PF25173 436 - 548 3.2e-12 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 436 - 549 2.9e-07 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 437 - 548 2.1e-08 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 438 - 550 9.7e-10 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 440 - 548 1.5e-09 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 440 - 548 5.3e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 443 - 550 3.3e-09 WDR3 second beta-propeller domain
Beta-prop_IFT140_1st PF23383 456 - 544 3.5e-06 IFT140 first beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 460 - 549 1.2e-07 WDR36/Utp21 first beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 462 - 549 1.2e-07 WDR90/POC16, second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 239
AccB1I GGYRCC 1 cut(s) 1628
AciI CCGC 3 cut(s) 298, 733, 1213
AclI AACGTT 1 cut(s) 1048
AclWI GGATC 3 cut(s) 816, 1067, 1109
AcsI RAATTY 3 cut(s) 757, 795, 1150
AfaI GTAC 3 cut(s) 131, 700, 1145
AfiI CCNNNNNNNGG 3 cut(s) 346, 1266, 1280
AflII CTTAAG 1 cut(s) 826
AgsI TTSAA 9 cut(s) 305, 372, 536, 745, 839, 1182, 1319, 1399, 1512
AhlI ACTAGT 2 cut(s) 506, 695
AjiI CACGTC 1 cut(s) 777
AjnI CCWGG 3 cut(s) 422, 442, 1005
AluBI AGCT 6 cut(s) 90, 259, 398, 951, 1020, 1198
AluI AGCT 6 cut(s) 90, 259, 398, 951, 1020, 1198
Alw21I GWGCWC 1 cut(s) 458
Alw26I GTCTC 2 cut(s) 1309, 1395
Alw44I GTGCAC 1 cut(s) 454
AlwI GGATC 3 cut(s) 816, 1067, 1109
AlwNI CAGNNNCTG 1 cut(s) 707
AoxI GGCC 2 cut(s) 1239, 1564
ApaLI GTGCAC 1 cut(s) 454
ApeKI GCWGC 1 cut(s) 767
ApoI RAATTY 3 cut(s) 757, 795, 1150
AseI ATTAAT 1 cut(s) 924
Asp700I GAANNNNTTC 1 cut(s) 1326
AspLEI GCGC 1 cut(s) 319
AspS9I GGNCC 1 cut(s) 143
AsuHPI GGTGA 2 cut(s) 530, 656
AvaII GGWCC 1 cut(s) 143
BaeGI GKGCMC 1 cut(s) 458
BanI GGYRCC 1 cut(s) 1628
BanII GRGCYC 1 cut(s) 422
Bbv12I GWGCWC 1 cut(s) 458
BbvCI CCTCAGC 2 cut(s) 708, 810
BbvI GCAGC 1 cut(s) 754
BccI CCATC 3 cut(s) 77, 377, 509
BceAI ACGGC 2 cut(s) 48, 173
BciT130I CCWGG 3 cut(s) 424, 444, 1007
BciVI GTATCC 2 cut(s) 937, 1658
BcoDI GTCTC 2 cut(s) 1309, 1395
BcuI ACTAGT 2 cut(s) 506, 695
BfaI CTAG 2 cut(s) 507, 696
BfmI CTRYAG 1 cut(s) 469
BfoI RGCGCY 1 cut(s) 320
BfrI CTTAAG 1 cut(s) 826
BfuI GTATCC 2 cut(s) 937, 1658
BisI GCNGC 2 cut(s) 768, 1213
BlsI GCNGC 2 cut(s) 769, 1214
Bme1390I CCNGG 3 cut(s) 424, 444, 1007
Bme18I GGWCC 1 cut(s) 143
BmgBI CACGTC 1 cut(s) 777
BmgT120I GGNCC 1 cut(s) 143
BmiI GGNNCC 5 cut(s) 421, 903, 985, 1211, 1630
BmrFI CCNGG 3 cut(s) 424, 444, 1007
BmsI GCATC 4 cut(s) 166, 694, 866, 1624
BpmI CTGGAG 1 cut(s) 406
Bpu10I CCTNAGC 2 cut(s) 708, 810
BpuEI CTTGAG 2 cut(s) 757, 1623
BsaJI CCNNGG 3 cut(s) 423, 443, 897
BsaXI ACNNNNNCTCC 4 cut(s) 937, 967, 1371, 1401
Bsc4I CCNNNNNNNGG 3 cut(s) 346, 1266, 1280
Bse1I ACTGG 2 cut(s) 1445, 1588
Bse3DI GCAATG 2 cut(s) 1222, 1335
BseBI CCWGG 3 cut(s) 424, 444, 1007
BseDI CCNNGG 3 cut(s) 423, 443, 897
BseGI GGATG 3 cut(s) 214, 857, 1468
BseLI CCNNNNNNNGG 3 cut(s) 346, 1266, 1280
BseMI GCAATG 2 cut(s) 1222, 1335
BseMII CTCAG 3 cut(s) 699, 801, 1320
BseNI ACTGG 2 cut(s) 1445, 1588
BseSI GKGCMC 1 cut(s) 458
BseXI GCAGC 1 cut(s) 754
BsgI GTGCAG 1 cut(s) 1211
BshFI GGCC 2 cut(s) 1241, 1566
BshNI GGYRCC 1 cut(s) 1628
BsiHKAI GWGCWC 1 cut(s) 458
BslFI GGGAC 1 cut(s) 517
BslI CCNNNNNNNGG 3 cut(s) 346, 1266, 1280
BsmAI GTCTC 2 cut(s) 1309, 1395
BsmFI GGGAC 1 cut(s) 517
BsmI GAATGC 1 cut(s) 1164
BsnI GGCC 2 cut(s) 1241, 1566
Bsp1286I GDGCHC 2 cut(s) 422, 458
Bsp1407I TGTACA 1 cut(s) 1143
Bsp143I GATC 5 cut(s) 151, 821, 845, 1072, 1114
Bsp19I CCATGG 1 cut(s) 897
BspACI CCGC 3 cut(s) 298, 733, 1213
BspANI GGCC 2 cut(s) 1241, 1566
BspCNI CTCAG 3 cut(s) 700, 802, 1319
BspHI TCATGA 1 cut(s) 310
BspLI GGNNCC 5 cut(s) 421, 903, 985, 1211, 1630
BspPI GGATC 3 cut(s) 816, 1067, 1109
BspT107I GGYRCC 1 cut(s) 1628
BspTI CTTAAG 1 cut(s) 826
BsrDI GCAATG 2 cut(s) 1222, 1335
BsrGI TGTACA 1 cut(s) 1143
BsrI ACTGG 2 cut(s) 1445, 1588
BssECI CCNNGG 3 cut(s) 423, 443, 897
BssMI GATC 5 cut(s) 151, 821, 845, 1072, 1114
BssT1I CCWWGG 1 cut(s) 897
Bst2UI CCWGG 3 cut(s) 424, 444, 1007
Bst4CI ACNGT 5 cut(s) 185, 473, 938, 1059, 1280
Bst6I CTCTTC 1 cut(s) 732
BstAFI CTTAAG 1 cut(s) 826
BstAUI TGTACA 1 cut(s) 1143
BstC8I GCNNGC 1 cut(s) 765
BstDEI CTNAG 6 cut(s) 202, 708, 810, 818, 1021, 1306
BstDSI CCRYGG 1 cut(s) 897
BstF5I GGATG 3 cut(s) 214, 857, 1468
BstH2I RGCGCY 1 cut(s) 320
BstHHI GCGC 1 cut(s) 319
BstKTI GATC 5 cut(s) 154, 824, 848, 1075, 1117
BstMAI GTCTC 2 cut(s) 1309, 1395
BstMBI GATC 5 cut(s) 151, 821, 845, 1072, 1114
BstNI CCWGG 3 cut(s) 424, 444, 1007
BstSCI CCNGG 3 cut(s) 422, 442, 1005
BstSFI CTRYAG 1 cut(s) 469
BstSLI GKGCMC 1 cut(s) 458
BstV1I GCAGC 1 cut(s) 754
BstX2I RGATCY 3 cut(s) 821, 1072, 1114
BstXI CCANNNNNNTGG 1 cut(s) 118
BstYI RGATCY 3 cut(s) 821, 1072, 1114
BsuI GTATCC 2 cut(s) 937, 1658
BsuRI GGCC 2 cut(s) 1241, 1566
BtgI CCRYGG 1 cut(s) 897
BtrI CACGTC 1 cut(s) 777
BtsCI GGATG 3 cut(s) 214, 857, 1468
BtsI GCAGTG 2 cut(s) 196, 455
BtsIMutI CAGTG 7 cut(s) 190, 196, 455, 478, 1088, 1285, 1452
Cac8I GCNNGC 1 cut(s) 765
CaiI CAGNNNCTG 1 cut(s) 707
CciI TCATGA 1 cut(s) 310
CfoI GCGC 1 cut(s) 319
Cfr13I GGNCC 1 cut(s) 143
Csp6I GTAC 3 cut(s) 130, 699, 1144
CviAII CATG 7 cut(s) 72, 311, 898, 1332, 1432, 1615, 1669
CviQI GTAC 3 cut(s) 130, 699, 1144
DdeI CTNAG 6 cut(s) 202, 708, 810, 818, 1021, 1306
DpnI GATC 5 cut(s) 153, 823, 847, 1074, 1116
DpnII GATC 5 cut(s) 151, 821, 845, 1072, 1114
Eam1104I CTCTTC 1 cut(s) 732
EarI CTCTTC 1 cut(s) 732
Eco130I CCWWGG 1 cut(s) 897
Eco24I GRGCYC 1 cut(s) 422
Eco47I GGWCC 1 cut(s) 143
EcoRII CCWGG 3 cut(s) 422, 442, 1005
EcoT14I CCWWGG 1 cut(s) 897
EcoT22I ATGCAT 2 cut(s) 22, 1337
EcoT38I GRGCYC 1 cut(s) 422
ErhI CCWWGG 1 cut(s) 897
FaeI CATG 7 cut(s) 75, 314, 901, 1335, 1435, 1618, 1672
FaqI GGGAC 1 cut(s) 517
FatI CATG 7 cut(s) 71, 310, 897, 1331, 1431, 1614, 1668
FauI CCCGC 1 cut(s) 740
Fnu4HI GCNGC 2 cut(s) 768, 1213
FokI GGATG 3 cut(s) 201, 844, 1455
FriOI GRGCYC 1 cut(s) 422
Fsp4HI GCNGC 2 cut(s) 768, 1213
FspBI CTAG 2 cut(s) 507, 696
GlaI GCGC 1 cut(s) 318
GluI GCNGC 2 cut(s) 768, 1213
GsuI CTGGAG 1 cut(s) 406
HaeII RGCGCY 1 cut(s) 320
HaeIII GGCC 2 cut(s) 1241, 1566
HhaI GCGC 1 cut(s) 319
Hin1II CATG 7 cut(s) 75, 314, 901, 1335, 1435, 1618, 1672
Hin6I GCGC 1 cut(s) 317
HinP1I GCGC 1 cut(s) 317
HincII GTYRAC 4 cut(s) 430, 913, 1452, 1603
HindII GTYRAC 4 cut(s) 430, 913, 1452, 1603
HindIII AAGCTT 2 cut(s) 88, 1018
HphI GGTGA 2 cut(s) 530, 656
Hpy166II GTNNAC 9 cut(s) 325, 430, 456, 716, 913, 934, 1146, 1452, 1603
Hpy188I TCNGA 8 cut(s) 584, 657, 723, 781, 906, 1036, 1063, 1309
Hpy188III TCNNGA 4 cut(s) 80, 311, 861, 1475
Hpy8I GTNNAC 9 cut(s) 325, 430, 456, 716, 913, 934, 1146, 1452, 1603
Hpy99I CGWCG 1 cut(s) 52
HpyAV CCTTC 5 cut(s) 299, 340, 746, 874, 1231
HpyCH4III ACNGT 5 cut(s) 185, 473, 938, 1059, 1280
HpyCH4IV ACGT 4 cut(s) 47, 776, 868, 1048
HpyCH4V TGCA 7 cut(s) 20, 104, 456, 607, 1192, 1335, 1340
HpyF3I CTNAG 6 cut(s) 202, 708, 810, 818, 1021, 1306
HpySE526I ACGT 4 cut(s) 47, 776, 868, 1048
Hsp92II CATG 7 cut(s) 75, 314, 901, 1335, 1435, 1618, 1672
HspAI GCGC 1 cut(s) 317
Kzo9I GATC 5 cut(s) 151, 821, 845, 1072, 1114
LmnI GCTCC 5 cut(s) 387, 425, 445, 983, 1209
Lsp1109I GCAGC 1 cut(s) 754
LweI GCATC 4 cut(s) 166, 694, 866, 1624
MaeI CTAG 2 cut(s) 507, 696
MaeII ACGT 4 cut(s) 47, 776, 868, 1048
MalI GATC 5 cut(s) 153, 823, 847, 1074, 1116
MboI GATC 5 cut(s) 151, 821, 845, 1072, 1114
MboII GAAGA 7 cut(s) 297, 585, 659, 719, 885, 1331, 1517
MfeI CAATTG 1 cut(s) 834
MflI RGATCY 3 cut(s) 821, 1072, 1114
MhlI GDGCHC 2 cut(s) 422, 458
MlyI GAGTC 3 cut(s) 718, 961, 1349
MmeI TCCRAC 2 cut(s) 262, 1041
Mph1103I ATGCAT 2 cut(s) 22, 1337
MroXI GAANNNNTTC 1 cut(s) 1326
MseI TTAA 8 cut(s) 92, 827, 924, 978, 1044, 1289, 1413, 1608
MspCI CTTAAG 1 cut(s) 826
MspR9I CCNGG 3 cut(s) 424, 444, 1007
MunI CAATTG 1 cut(s) 834
Mva1269I GAATGC 1 cut(s) 1164
MvaI CCWGG 3 cut(s) 424, 444, 1007
NcoI CCATGG 1 cut(s) 897
NdeII GATC 5 cut(s) 151, 821, 845, 1072, 1114
NlaIII CATG 7 cut(s) 75, 314, 901, 1335, 1435, 1618, 1672
NlaIV GGNNCC 5 cut(s) 421, 903, 985, 1211, 1630
NsiI ATGCAT 2 cut(s) 22, 1337
PagI TCATGA 1 cut(s) 310
PctI GAATGC 1 cut(s) 1164
PdmI GAANNNNTTC 1 cut(s) 1326
PfeI GAWTC 7 cut(s) 76, 521, 579, 741, 789, 989, 1508
PkrI GCNGC 2 cut(s) 769, 1214
PleI GAGTC 3 cut(s) 718, 961, 1349
PpsI GAGTC 3 cut(s) 718, 961, 1349
PshBI ATTAAT 1 cut(s) 924
PsiI TTATAA 1 cut(s) 239
Psp1406I AACGTT 1 cut(s) 1048
Psp6I CCWGG 3 cut(s) 422, 442, 1005
PspGI CCWGG 3 cut(s) 422, 442, 1005
PspN4I GGNNCC 5 cut(s) 421, 903, 985, 1211, 1630
PspPI GGNCC 1 cut(s) 143
PsrI GAACNNNNNNTAC 2 cut(s) 1062, 1094
PstNI CAGNNNCTG 1 cut(s) 707
PsuI RGATCY 3 cut(s) 821, 1072, 1114
RsaI GTAC 3 cut(s) 131, 700, 1145
RsaNI GTAC 3 cut(s) 130, 699, 1144
SaqAI TTAA 8 cut(s) 92, 827, 924, 978, 1044, 1289, 1413, 1608
SatI GCNGC 2 cut(s) 768, 1213
Sau3AI GATC 5 cut(s) 151, 821, 845, 1072, 1114
Sau96I GGNCC 1 cut(s) 143
SchI GAGTC 3 cut(s) 718, 961, 1349
ScrFI CCNGG 3 cut(s) 424, 444, 1007
SduI GDGCHC 2 cut(s) 422, 458
SfaNI GCATC 4 cut(s) 166, 694, 866, 1624
SfcI CTRYAG 1 cut(s) 469
SinI GGWCC 1 cut(s) 143
SmlI CTYRAG 3 cut(s) 736, 826, 1638
SmoI CTYRAG 3 cut(s) 736, 826, 1638
SpeI ACTAGT 2 cut(s) 506, 695
SsiI CCGC 3 cut(s) 298, 733, 1213
SspI AATATT 1 cut(s) 355
SspMI CTAG 2 cut(s) 507, 696
StyD4I CCNGG 3 cut(s) 422, 442, 1005
StyI CCWWGG 1 cut(s) 897
TaaI ACNGT 5 cut(s) 185, 473, 938, 1059, 1280
TaiI ACGT 4 cut(s) 50, 779, 871, 1051
TaqI TCGA 5 cut(s) 50, 844, 1087, 1135, 1534
TatI WGTACW 3 cut(s) 129, 698, 1143
TauI GCSGC 1 cut(s) 1215
TfiI GAWTC 7 cut(s) 76, 521, 579, 741, 789, 989, 1508
Tru1I TTAA 8 cut(s) 92, 827, 924, 978, 1044, 1289, 1413, 1608
Tru9I TTAA 8 cut(s) 92, 827, 924, 978, 1044, 1289, 1413, 1608
TscAI CASTG 7 cut(s) 190, 196, 455, 478, 1088, 1285, 1452
TseI GCWGC 1 cut(s) 767
TspDTI ATGAA 9 cut(s) 113, 327, 558, 867, 893, 1233, 1420, 1457, 1611
TspGWI ACGGA 2 cut(s) 495, 801
TspRI CASTG 7 cut(s) 190, 196, 455, 478, 1088, 1285, 1452
Vha464I CTTAAG 1 cut(s) 826
VneI GTGCAC 1 cut(s) 454
VpaK11BI GGWCC 1 cut(s) 143
VspI ATTAAT 1 cut(s) 924
XapI RAATTY 3 cut(s) 757, 795, 1150
XmnI GAANNNNTTC 1 cut(s) 1326
XspI CTAG 2 cut(s) 507, 696
Zsp2I ATGCAT 2 cut(s) 22, 1337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.