pycom13g14410
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0010022
Physical Location & Seq
Reverse (-)
10400249 .. 10404862
4614 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1635 bp
ATGGAGAGCTCTGTAGGAGAAGCAGCGAAGAAGCAGCAGGAGTTTGCTTTGGCATCCGCCTCCGAGCTCGCTTCGTTGTCGTCTTCTACTACTCTGGCACCGGCGGTGGCTCGGTTCAGTCCCGAAGACGGAGTTGCAGAGCTCCGGTTTCAGCAGGAGGCTGAGTCCGATGCCCGCCTCAATATCGATCTCCAAACTGCTAAGCTATTCAAGTTAGGCCCGGTGCAGTCAGTTTGCATAACAGAAAGTTCTGATACCGATGAAGAGAAATCGTATTCAAGGGGAGTCACTATTCGTTTTAATAACGAGGAGGAGAGTGCAGCCTTCCATCATGCATTCGAGCAATGGAAGAAGGACCTTGTTGATCAAGGACAAAAGTTACCAAATGGAGAACTGTCATCTACCAAAAGCAAGTTTGACGACAAAATAGAGCCATCTTCTGCTAAAATGTATTTCCATTACTATGGACAATTGCTACATCAACAGAACATGTTACAGGATTATGTTAGGACAGGAACCTATTATGCTGCTGTCATAGAAAATCGTGTGGATTTTAATGGTCGCGTGGTTGTTGATGTTGGTGCTGGTAGTGGTATTTTGTCATTATTTGCTGCACAGGCTGGTGCTAAACATGTTTATGCTGTGGAAGCATCTGAAATGGCAGATTATGCACGCAAGCTAATTGCAGGGAACCCTTCACTTGGTGAAAGGATAACTGTAATCAAAGGTAAAATTGAGGAGGTTGACTTGCCAGAGAAAGCAGATATCCTTATCTCAGAGCCAATGGGCACTTTGTTAATTAATGAAAGAATGCTGGAAACCTATGTGATTGCAAGAGATCGGTTTCTTCAACCAAATGGGAAAATGTTCCCCACATTGGGAAGGATTCACATGGCACCTTTCAGTGAAGAATATTTGTTTGTTGAAATAGCAAATAAGGCCCTCTTCTGGCAGCAACAAAATTATTATGGAGTTGATTTGCAACCGTTGTATGGATCTGCATTCCAGGGATATTTTTCACAGCCTGTGGTAGATGCTTTTGATCCAAGATTACTGGTGGCTCCTTCAATGTCTCATGTGATTGACTTTACCAAAACAAGCGAAGAGGACCTGTATGAAATCGACATACCTTTACGGTTTACAGCTGCTGTAGGCACTAGAGTGCATGGGTTAGCATGCTGGTTCGATGTCTTGTTTGATGGGAGTACCGTACAAAGGTGGCTTACCACCGCGCCTGGTGCACCAACGACCCATTGGTACCAATTACGCTGCGTTCTCTCTCAGCCAATTTATGTGATGGCAGGACAAGAAATCACTGGCCGACTCCACATGGTTGCCCACAATTCTCAGAGTTACACAATATATCTCACTTTGGCAGCTAAAATGTGGGGCCCTGGGGCTGAGCTAGGAGGTGTAATTCAGCAGTCTTCTTGTAAACTTGATCTCAAAGAGCCGTACTATAGAATGTCGCAACCGCAAGCCTACACAATTGCCCAAGATCAGCAACCACATCAGCAAATACAGACACAGGATTTGGACGATTCTGAATTTATTGCACAACCCTCACCGACTTCAGGCTCAAAGATCTTACTGGAAGAATTTGCCAAATCCGCGGGGGCTTTGAAGAACATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006807 GO:0006950 GO:0006970 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008213 GO:0008276 GO:0008469 GO:0008757 GO:0009628 GO:0009651 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009909 GO:0009987 GO:0010035 GO:0010038 GO:0010228 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016273 GO:0016274 GO:0016277 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018193 GO:0018195 GO:0018216 GO:0019219 GO:0019222 GO:0019538 GO:0019919 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032259 GO:0032501 GO:0032502 GO:0034969 GO:0034970 GO:0034971 GO:0034972 GO:0035241 GO:0035242 GO:0035246 GO:0035247 GO:0036211 GO:0042054 GO:0042221 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045935 GO:0046686 GO:0046982 GO:0046983 GO:0048518 GO:0048522 GO:0048580 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051239 GO:0051252 GO:0051254 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0140096 GO:1901564 GO:1902680 GO:1902882 GO:1902884 GO:1903506 GO:1903508 GO:2000026 GO:2000112 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko01522 map01522
Pfam Domains
Protein Families
Methyltransf_25 PrmA

Protein Analysis

545

Amino Acids

60.56

Weight (kDa)

5.06

Isoelectric Point (pI)

53.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH_PRMT_N PF25350 12 - 118 1.5e-42 Protein arginine methyltransferase N-terminal PH domain
PrmA PF06325 184 - 259 1.7e-07 Ribosomal protein L11 methyltransferase (PrmA)
Methyltransf_25 PF13649 190 - 287 5.2e-07 Methyltransferase domain
PRMT_C PF22528 311 - 450 3.8e-20 Arginine methyltransferase oligomerization subdomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1257
AccB1I GGYRCC 3 cut(s) 97, 895, 1257
AccII CGCG 3 cut(s) 564, 1232, 1613
AciI CCGC 7 cut(s) 57, 104, 175, 1230, 1475, 1611, 1613
AclWI GGATC 2 cut(s) 1003, 1037
AcoI YGGCCR 1 cut(s) 1318
AcsI RAATTY 2 cut(s) 1547, 1598
AcuI CTGAAG 1 cut(s) 1557
AdeI CACNNNGTG 1 cut(s) 704
AfaI GTAC 4 cut(s) 1207, 1212, 1259, 1457
AfiI CCNNNNNNNGG 8 cut(s) 128, 701, 877, 878, 948, 992, 1215, 1574
AflIII ACRYGT 2 cut(s) 489, 631
AgsI TTSAA 6 cut(s) 211, 279, 851, 926, 1068, 1624
AjnI CCWGG 3 cut(s) 1005, 1234, 1393
AleI CACNNNNGTG 1 cut(s) 1160
AluBI AGCT 8 cut(s) 9, 67, 142, 205, 679, 1145, 1379, 1405
AluI AGCT 8 cut(s) 9, 67, 142, 205, 679, 1145, 1379, 1405
Alw21I GWGCWC 4 cut(s) 11, 69, 144, 1243
Alw26I GTCTC 1 cut(s) 1077
Alw44I GTGCAC 1 cut(s) 1239
AlwI GGATC 2 cut(s) 1003, 1037
AlwNI CAGNNNCTG 1 cut(s) 1148
AoxI GGCC 4 cut(s) 217, 939, 1318, 1390
ApaI GGGCCC 1 cut(s) 1394
ApaLI GTGCAC 1 cut(s) 1239
ApeKI GCWGC 9 cut(s) 23, 34, 320, 527, 611, 952, 1145, 1269, 1376
ApoI RAATTY 2 cut(s) 1547, 1598
ArsI GACNNNNNNTTYG 2 cut(s) 1516, 1548
AseI ATTAAT 1 cut(s) 801
Asp700I GAANNNNTTC 1 cut(s) 866
Asp718I GGTACC 1 cut(s) 1257
AspLEI GCGC 1 cut(s) 1234
AspS9I GGNCC 6 cut(s) 218, 355, 940, 1108, 1390, 1391
AsuC2I CCSGG 1 cut(s) 221
AsuHPI GGTGA 2 cut(s) 716, 1557
AvaII GGWCC 2 cut(s) 355, 1108
BaeGI GKGCMC 3 cut(s) 791, 1243, 1394
BanI GGYRCC 3 cut(s) 97, 895, 1257
BanII GRGCYC 4 cut(s) 11, 69, 144, 1394
BbsI GAAGAC 3 cut(s) 75, 132, 1419
Bbv12I GWGCWC 4 cut(s) 11, 69, 144, 1243
BbvI GCAGC 9 cut(s) 35, 46, 332, 514, 598, 964, 1132, 1256, 1388
BccI CCATC 4 cut(s) 336, 442, 1193, 1291
BceAI ACGGC 1 cut(s) 1438
BciT130I CCWGG 3 cut(s) 1007, 1236, 1395
BclI TGATCA 1 cut(s) 364
BcnI CCSGG 1 cut(s) 221
BcoDI GTCTC 1 cut(s) 1077
BfaI CTAG 2 cut(s) 1158, 1406
BfmI CTRYAG 3 cut(s) 12, 1149, 1459
BglII AGATCT 1 cut(s) 1584
BisI GCNGC 9 cut(s) 24, 35, 321, 528, 612, 953, 1146, 1270, 1377
BlpI GCTNAGC 2 cut(s) 201, 1401
BlsI GCNGC 9 cut(s) 25, 36, 322, 529, 613, 954, 1147, 1271, 1378
Bme1390I CCNGG 4 cut(s) 221, 1007, 1236, 1395
Bme18I GGWCC 2 cut(s) 355, 1108
BmgT120I GGNCC 6 cut(s) 218, 355, 940, 1108, 1390, 1391
BmiI GGNNCC 8 cut(s) 99, 517, 692, 897, 1062, 1259, 1391, 1392
BmrFI CCNGG 4 cut(s) 221, 1007, 1236, 1395
BmsI GCATC 4 cut(s) 62, 160, 659, 1024
BpiI GAAGAC 3 cut(s) 75, 132, 1419
Bpu1102I GCTNAGC 2 cut(s) 201, 1401
BpuMI CCSGG 1 cut(s) 221
Bsa29I ATCGAT 1 cut(s) 186
BsaJI CCNNGG 4 cut(s) 1006, 1393, 1394, 1611
BsaWI WCCGGW 1 cut(s) 144
BsaXI ACNNNNNCTCC 2 cut(s) 149, 179
Bsc4I CCNNNNNNNGG 8 cut(s) 128, 701, 877, 878, 948, 992, 1215, 1574
Bse118I RCCGGY 1 cut(s) 100
Bse1I ACTGG 3 cut(s) 1059, 1321, 1596
Bse3DI GCAATG 1 cut(s) 350
BseBI CCWGG 3 cut(s) 1007, 1236, 1395
BseCI ATCGAT 1 cut(s) 186
BseDI CCNNGG 4 cut(s) 1006, 1393, 1394, 1611
BseGI GGATG 1 cut(s) 53
BseLI CCNNNNNNNGG 8 cut(s) 128, 701, 877, 878, 948, 992, 1215, 1574
BseMI GCAATG 1 cut(s) 350
BseMII CTCAG 5 cut(s) 153, 789, 1295, 1361, 1392
BseNI ACTGG 3 cut(s) 1059, 1321, 1596
BseRI GAGGAG 3 cut(s) 323, 326, 752
BseSI GKGCMC 3 cut(s) 791, 1243, 1394
BseXI GCAGC 9 cut(s) 35, 46, 332, 514, 598, 964, 1132, 1256, 1388
BsgI GTGCAG 3 cut(s) 245, 339, 597
Bsh1236I CGCG 3 cut(s) 564, 1232, 1613
BshFI GGCC 4 cut(s) 219, 941, 1320, 1392
BshNI GGYRCC 3 cut(s) 97, 895, 1257
BshVI ATCGAT 1 cut(s) 186
BsiHKAI GWGCWC 4 cut(s) 11, 69, 144, 1243
BsiSI CCGG 3 cut(s) 101, 145, 221
BslFI GGGAC 1 cut(s) 105
BslI CCNNNNNNNGG 8 cut(s) 128, 701, 877, 878, 948, 992, 1215, 1574
BsmAI GTCTC 1 cut(s) 1077
BsmFI GGGAC 1 cut(s) 105
BsmI GAATGC 3 cut(s) 335, 816, 1001
BsnI GGCC 4 cut(s) 219, 941, 1320, 1392
Bsp120I GGGCCC 1 cut(s) 1390
Bsp1286I GDGCHC 6 cut(s) 11, 69, 144, 791, 1243, 1394
Bsp143I GATC 8 cut(s) 187, 364, 838, 995, 1042, 1441, 1498, 1584
Bsp1720I GCTNAGC 2 cut(s) 201, 1401
BspACI CCGC 7 cut(s) 57, 104, 175, 1230, 1475, 1611, 1613
BspANI GGCC 4 cut(s) 219, 941, 1320, 1392
BspCNI CTCAG 5 cut(s) 154, 788, 1294, 1360, 1393
BspDI ATCGAT 1 cut(s) 186
BspFNI CGCG 3 cut(s) 564, 1232, 1613
BspLI GGNNCC 8 cut(s) 99, 517, 692, 897, 1062, 1259, 1391, 1392
BspPI GGATC 2 cut(s) 1003, 1037
BspT107I GGYRCC 3 cut(s) 97, 895, 1257
BsrDI GCAATG 1 cut(s) 350
BsrFI RCCGGY 1 cut(s) 100
BsrI ACTGG 3 cut(s) 1059, 1321, 1596
BssAI RCCGGY 1 cut(s) 100
BssECI CCNNGG 4 cut(s) 1006, 1393, 1394, 1611
BssMI GATC 8 cut(s) 187, 364, 838, 995, 1042, 1441, 1498, 1584
Bst2UI CCWGG 3 cut(s) 1007, 1236, 1395
Bst4CI ACNGT 5 cut(s) 396, 718, 987, 1137, 1210
Bst6I CTCTTC 3 cut(s) 258, 950, 1098
BstAPI GCANNNNNTGC 1 cut(s) 668
BstC8I GCNNGC 6 cut(s) 69, 175, 673, 677, 1177, 1479
BstDEI CTNAG 6 cut(s) 162, 201, 775, 1281, 1347, 1401
BstDSI CCRYGG 1 cut(s) 1611
BstF5I GGATG 1 cut(s) 53
BstFNI CGCG 3 cut(s) 564, 1232, 1613
BstHHI GCGC 1 cut(s) 1234
BstKTI GATC 8 cut(s) 190, 367, 841, 998, 1045, 1444, 1501, 1587
BstMAI GTCTC 1 cut(s) 1077
BstMBI GATC 8 cut(s) 187, 364, 838, 995, 1042, 1441, 1498, 1584
BstMWI GCNNNNNNNGC 6 cut(s) 617, 647, 668, 938, 1238, 1610
BstNI CCWGG 3 cut(s) 1007, 1236, 1395
BstNSI RCATGY 3 cut(s) 493, 635, 1179
BstSCI CCNGG 4 cut(s) 219, 1005, 1234, 1393
BstSFI CTRYAG 3 cut(s) 12, 1149, 1459
BstSLI GKGCMC 3 cut(s) 791, 1243, 1394
BstUI CGCG 3 cut(s) 564, 1232, 1613
BstV1I GCAGC 9 cut(s) 35, 46, 332, 514, 598, 964, 1132, 1256, 1388
BstV2I GAAGAC 3 cut(s) 75, 132, 1419
BstX2I RGATCY 2 cut(s) 995, 1584
BstXI CCANNNNNNTGG 1 cut(s) 464
BstYI RGATCY 2 cut(s) 995, 1584
Bsu15I ATCGAT 1 cut(s) 186
BsuRI GGCC 4 cut(s) 219, 941, 1320, 1392
BsuTUI ATCGAT 1 cut(s) 186
BtgI CCRYGG 1 cut(s) 1611
BtsCI GGATG 1 cut(s) 53
BtsIMutI CAGTG 2 cut(s) 910, 1314
Cac8I GCNNGC 6 cut(s) 69, 175, 673, 677, 1177, 1479
CaiI CAGNNNCTG 1 cut(s) 1148
CfoI GCGC 1 cut(s) 1234
Cfr10I RCCGGY 1 cut(s) 100
Cfr13I GGNCC 6 cut(s) 218, 355, 940, 1108, 1390, 1391
Cfr42I CCGCGG 1 cut(s) 1614
ClaI ATCGAT 1 cut(s) 186
Csp6I GTAC 4 cut(s) 1206, 1211, 1258, 1456
CviAII CATG 8 cut(s) 332, 490, 632, 892, 1076, 1166, 1176, 1330
CviQI GTAC 4 cut(s) 1206, 1211, 1258, 1456
DdeI CTNAG 6 cut(s) 162, 201, 775, 1281, 1347, 1401
DpnI GATC 8 cut(s) 189, 366, 840, 997, 1044, 1443, 1500, 1586
DpnII GATC 8 cut(s) 187, 364, 838, 995, 1042, 1441, 1498, 1584
DraIII CACNNNGTG 1 cut(s) 704
EaeI YGGCCR 1 cut(s) 1318
Eam1104I CTCTTC 3 cut(s) 258, 950, 1098
EarI CTCTTC 3 cut(s) 258, 950, 1098
EciI GGCGGA 1 cut(s) 46
Ecl136II GAGCTC 3 cut(s) 9, 67, 142
Eco24I GRGCYC 4 cut(s) 11, 69, 144, 1394
Eco32I GATATC 1 cut(s) 766
Eco47I GGWCC 2 cut(s) 355, 1108
Eco53kI GAGCTC 3 cut(s) 9, 67, 142
Eco57I CTGAAG 1 cut(s) 1557
EcoICRI GAGCTC 3 cut(s) 9, 67, 142
EcoO109I RGGNCCY 5 cut(s) 355, 940, 1108, 1390, 1391
EcoRII CCWGG 3 cut(s) 1005, 1234, 1393
EcoRV GATATC 1 cut(s) 766
EcoT22I ATGCAT 1 cut(s) 337
EcoT38I GRGCYC 4 cut(s) 11, 69, 144, 1394
FaeI CATG 8 cut(s) 335, 493, 635, 895, 1079, 1169, 1179, 1333
FalI AAGNNNNNCTT 2 cut(s) 929, 961
FaqI GGGAC 1 cut(s) 105
FatI CATG 8 cut(s) 331, 489, 631, 891, 1075, 1165, 1175, 1329
FauI CCCGC 2 cut(s) 182, 1606
FbaI TGATCA 1 cut(s) 364
Fnu4HI GCNGC 9 cut(s) 24, 35, 321, 528, 612, 953, 1146, 1270, 1377
FokI GGATG 1 cut(s) 40
FriOI GRGCYC 4 cut(s) 11, 69, 144, 1394
Fsp4HI GCNGC 9 cut(s) 24, 35, 321, 528, 612, 953, 1146, 1270, 1377
FspBI CTAG 2 cut(s) 1158, 1406
GlaI GCGC 1 cut(s) 1233
GluI GCNGC 9 cut(s) 24, 35, 321, 528, 612, 953, 1146, 1270, 1377
HaeIII GGCC 4 cut(s) 219, 941, 1320, 1392
HapII CCGG 3 cut(s) 101, 145, 221
HhaI GCGC 1 cut(s) 1234
Hin1II CATG 8 cut(s) 335, 493, 635, 895, 1079, 1169, 1179, 1333
Hin6I GCGC 1 cut(s) 1232
HinP1I GCGC 1 cut(s) 1232
HincII GTYRAC 1 cut(s) 745
HindII GTYRAC 1 cut(s) 745
HinfI GANTC 5 cut(s) 164, 285, 886, 1323, 1541
HpaII CCGG 3 cut(s) 101, 145, 221
HphI GGTGA 2 cut(s) 716, 1557
Hpy166II GTNNAC 4 cut(s) 745, 1140, 1241, 1436
Hpy188I TCNGA 7 cut(s) 64, 169, 253, 655, 778, 1350, 1546
Hpy188III TCNNGA 1 cut(s) 122
Hpy8I GTNNAC 4 cut(s) 745, 1140, 1241, 1436
HpyAV CCTTC 5 cut(s) 334, 346, 705, 876, 1074
HpyCH4III ACNGT 5 cut(s) 396, 718, 987, 1137, 1210
HpyF10VI GCNNNNNNNGC 6 cut(s) 617, 647, 668, 938, 1238, 1610
HpyF3I CTNAG 6 cut(s) 162, 201, 775, 1281, 1347, 1401
Hsp92II CATG 8 cut(s) 335, 493, 635, 895, 1079, 1169, 1179, 1333
HspAI GCGC 1 cut(s) 1232
KpnI GGTACC 1 cut(s) 1261
Ksp22I TGATCA 1 cut(s) 364
KspI CCGCGG 1 cut(s) 1614
Kzo9I GATC 8 cut(s) 187, 364, 838, 995, 1042, 1441, 1498, 1584
LmnI GCTCC 2 cut(s) 147, 1066
Lsp1109I GCAGC 9 cut(s) 35, 46, 332, 514, 598, 964, 1132, 1256, 1388
LweI GCATC 4 cut(s) 62, 160, 659, 1024
MaeI CTAG 2 cut(s) 1158, 1406
MaeIII GTNAC 4 cut(s) 286, 378, 492, 1352
MalI GATC 8 cut(s) 189, 366, 840, 997, 1044, 1443, 1500, 1586
MboI GATC 8 cut(s) 187, 364, 838, 995, 1042, 1441, 1498, 1584
MfeI CAATTG 2 cut(s) 470, 1488
MflI RGATCY 2 cut(s) 995, 1584
MhlI GDGCHC 6 cut(s) 11, 69, 144, 791, 1243, 1394
MlyI GAGTC 3 cut(s) 173, 294, 1317
Mph1103I ATGCAT 1 cut(s) 337
MroXI GAANNNNTTC 1 cut(s) 866
MseI TTAA 4 cut(s) 300, 555, 797, 801
MslI CAYNNNNRTG 3 cut(s) 462, 636, 1160
MspA1I CMGCKG 2 cut(s) 1145, 1613
MspI CCGG 3 cut(s) 101, 145, 221
MspR9I CCNGG 4 cut(s) 221, 1007, 1236, 1395
MunI CAATTG 2 cut(s) 470, 1488
Mva1269I GAATGC 3 cut(s) 335, 816, 1001
MvaI CCWGG 3 cut(s) 1007, 1236, 1395
MvnI CGCG 3 cut(s) 564, 1232, 1613
MwoI GCNNNNNNNGC 6 cut(s) 617, 647, 668, 938, 1238, 1610
NciI CCSGG 1 cut(s) 221
NdeII GATC 8 cut(s) 187, 364, 838, 995, 1042, 1441, 1498, 1584
NlaIII CATG 8 cut(s) 335, 493, 635, 895, 1079, 1169, 1179, 1333
NlaIV GGNNCC 8 cut(s) 99, 517, 692, 897, 1062, 1259, 1391, 1392
NmuCI GTSAC 1 cut(s) 286
NsiI ATGCAT 1 cut(s) 337
NspI RCATGY 3 cut(s) 493, 635, 1179
OliI CACNNNNGTG 1 cut(s) 1160
PacI TTAATTAA 1 cut(s) 801
PaeI GCATGC 1 cut(s) 1179
PasI CCCWGGG 1 cut(s) 1394
PciI ACATGT 2 cut(s) 489, 631
PctI GAATGC 3 cut(s) 335, 816, 1001
PdmI GAANNNNTTC 1 cut(s) 866
PfeI GAWTC 2 cut(s) 886, 1541
PkrI GCNGC 9 cut(s) 25, 36, 322, 529, 613, 954, 1147, 1271, 1378
PleI GAGTC 3 cut(s) 172, 293, 1317
PpsI GAGTC 3 cut(s) 172, 293, 1317
PpuMI RGGWCCY 2 cut(s) 355, 1108
PscI ACATGT 2 cut(s) 489, 631
PshBI ATTAAT 1 cut(s) 801
Psp124BI GAGCTC 3 cut(s) 11, 69, 144
Psp5II RGGWCCY 2 cut(s) 355, 1108
Psp6I CCWGG 3 cut(s) 1005, 1234, 1393
PspGI CCWGG 3 cut(s) 1005, 1234, 1393
PspN4I GGNNCC 8 cut(s) 99, 517, 692, 897, 1062, 1259, 1391, 1392
PspOMI GGGCCC 1 cut(s) 1390
PspPI GGNCC 6 cut(s) 218, 355, 940, 1108, 1390, 1391
PspPPI RGGWCCY 2 cut(s) 355, 1108
PstNI CAGNNNCTG 1 cut(s) 1148
PsuI RGATCY 2 cut(s) 995, 1584
PvuII CAGCTG 1 cut(s) 1145
RsaI GTAC 4 cut(s) 1207, 1212, 1259, 1457
RsaNI GTAC 4 cut(s) 1206, 1211, 1258, 1456
RseI CAYNNNNRTG 3 cut(s) 462, 636, 1160
SacI GAGCTC 3 cut(s) 11, 69, 144
SacII CCGCGG 1 cut(s) 1614
SaqAI TTAA 4 cut(s) 300, 555, 797, 801
SatI GCNGC 9 cut(s) 24, 35, 321, 528, 612, 953, 1146, 1270, 1377
Sau3AI GATC 8 cut(s) 187, 364, 838, 995, 1042, 1441, 1498, 1584
Sau96I GGNCC 6 cut(s) 218, 355, 940, 1108, 1390, 1391
SchI GAGTC 3 cut(s) 173, 294, 1317
ScrFI CCNGG 4 cut(s) 221, 1007, 1236, 1395
SduI GDGCHC 6 cut(s) 11, 69, 144, 791, 1243, 1394
SfaNI GCATC 4 cut(s) 62, 160, 659, 1024
SfcI CTRYAG 3 cut(s) 12, 1149, 1459
Sfr303I CCGCGG 1 cut(s) 1614
SgrAI CRCCGGYG 1 cut(s) 100
SgrBI CCGCGG 1 cut(s) 1614
SinI GGWCC 2 cut(s) 355, 1108
SmiMI CAYNNNNRTG 3 cut(s) 462, 636, 1160
SphI GCATGC 1 cut(s) 1179
SsiI CCGC 7 cut(s) 57, 104, 175, 1230, 1475, 1611, 1613
SspI AATATT 1 cut(s) 914
SspMI CTAG 2 cut(s) 1158, 1406
SstI GAGCTC 3 cut(s) 11, 69, 144
StyD4I CCNGG 4 cut(s) 219, 1005, 1234, 1393
TaaI ACNGT 5 cut(s) 396, 718, 987, 1137, 1210
TaqI TCGA 4 cut(s) 186, 339, 1122, 1185
TfiI GAWTC 2 cut(s) 886, 1541
Tru1I TTAA 4 cut(s) 300, 555, 797, 801
Tru9I TTAA 4 cut(s) 300, 555, 797, 801
TscAI CASTG 2 cut(s) 910, 1321
TseFI GTSAC 1 cut(s) 286
TseI GCWGC 9 cut(s) 23, 34, 320, 527, 611, 952, 1145, 1269, 1376
Tsp45I GTSAC 1 cut(s) 286
TspDTI ATGAA 3 cut(s) 276, 819, 1131
TspGWI ACGGA 1 cut(s) 144
TspRI CASTG 2 cut(s) 910, 1321
VneI GTGCAC 1 cut(s) 1239
VpaK11BI GGWCC 2 cut(s) 355, 1108
VspI ATTAAT 1 cut(s) 801
XapI RAATTY 2 cut(s) 1547, 1598
XceI RCATGY 3 cut(s) 493, 635, 1179
XcmI CCANNNNNNNNNTGG 1 cut(s) 1251
XmnI GAANNNNTTC 1 cut(s) 866
XspI CTAG 2 cut(s) 1158, 1406
Zsp2I ATGCAT 1 cut(s) 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.