pycom13g17950

Zeta toxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0007045
Physical Location & Seq
Reverse (-)
13892856 .. 13899565
6710 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1119 bp
ATGCAGCACCATGAAGGTGGAAATGGAAAAATCAACCTCACAGTACACATCTTGGCTGCGTCCTTTGTTGGGATTCTGGCTGCTGCTGCTGCTGCCACGAGCAGTTATCGTCGGAAAAAGTCGTATAAGGCGTTGAGAGCTCCAAACAATATCTTTCCACGGATAGAGAGGACGGAATCCGGCCGCGTTGGAAATATCGAAAGATTCTCCCATTATGTCGCTAGGCAACTGGGAATTTTAGATGCAAATAAGTGCCCCCAGCTGTGCAAGTTAGCCTATGACTACTTGAGAAAAACGAAAGGGTGTGAAGATAACATCTACGAATATTTCGCTTCAGAAGCAGATCCAGATTCTCTCTACATAAATTTGATTGATGAATTTGATAGATGCATCCTTAGCTATTTTGCATTTCATTGGAGCCAAGCTACTATAACGATTAGTCAGGTTTTGAATGTAGAGACTAAGAAGAAAACGAAGCTCAAAGACATGGTATTGGCAGCTACCAGGAAGGAGAGATTTGAGAGGATCAATAAGGAGCTAAAGATGACAAGGGTGTTCTCTACGCTGGTAGATGAAATGAAAGCCATCGGAAGCACTTCAATAAAGGGAGAATCACAATGCACGGACGTGATGAAGCCGGTGGCCCATAATGAGAGGAGTCCCGTGCTTCTTCTTATGGGTGGTGGAATGGGGGGTGGCAAAAGCACTGTCCTCAAAGATATTCTCAAAGAGTCATTCTGGTCTGGAGCAGCAGCAGATGCTGTGGTAGTAGAGGCAGATGCTTTCAAAGAGACAGACGTCCTTTACAGAGCCCTTAGCTCTAGAGGTCACCACAGCGACATGCTCCAAACTGCTGAGCTAGTGCACCAATCATCTACTGATGCTGCATCATCGCTTCTAGTGACTGCACTTAATGAAGGAAGGGATGTGATCATGGACGGCACTCTATCATGGATGCCCTTTGTTGAGCAGACAATAACCATGGCCCGGGATGTCCACAAATGCCGCTACAGAATGGGGGTCGGTTACAAGGTGGAGGAAGACGGCACCATTACTGAAAACTATTGGGAACGAGTGGACGATGGAGAGGATCGATCAGGATCAGCAGAAGATAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zeta_toxin

Protein Analysis

373

Amino Acids

41.56

Weight (kDa)

6.57

Isoelectric Point (pI)

40.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zeta_toxin PF06414 213 - 327 7.2e-13 Zeta toxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 801
AccB1I GGYRCC 1 cut(s) 1046
AccII CGCG 1 cut(s) 186
AciI CCGC 2 cut(s) 184, 1006
AclWI GGATC 4 cut(s) 338, 533, 1098, 1108
AcoI YGGCCR 1 cut(s) 181
AcsI RAATTY 3 cut(s) 234, 364, 377
AcuI CTGAAG 1 cut(s) 318
AcyI GRCGYC 1 cut(s) 798
AfaI GTAC 1 cut(s) 45
AfiI CCNNNNNNNGG 2 cut(s) 69, 987
AgsI TTSAA 3 cut(s) 451, 600, 787
AjiI CACGTC 1 cut(s) 628
AjnI CCWGG 1 cut(s) 503
AleI CACNNNNGTG 1 cut(s) 626
AluBI AGCT 9 cut(s) 140, 262, 399, 425, 478, 500, 538, 819, 859
AluI AGCT 9 cut(s) 140, 262, 399, 425, 478, 500, 538, 819, 859
Alw21I GWGCWC 2 cut(s) 142, 867
Alw26I GTCTC 2 cut(s) 452, 785
Alw44I GTGCAC 1 cut(s) 863
AlwI GGATC 4 cut(s) 338, 533, 1098, 1108
AlwNI CAGNNNCTG 1 cut(s) 761
Ama87I CYCGRG 1 cut(s) 987
AoxI GGCC 3 cut(s) 181, 642, 984
ApaLI GTGCAC 1 cut(s) 863
ApoI RAATTY 3 cut(s) 234, 364, 377
ArsI GACNNNNNNTTYG 2 cut(s) 693, 725
Asp700I GAANNNNTTC 1 cut(s) 595
AspS9I GGNCC 2 cut(s) 643, 985
AsuC2I CCSGG 2 cut(s) 988, 989
AsuHPI GGTGA 1 cut(s) 821
AvaI CYCGRG 1 cut(s) 987
BaeGI GKGCMC 2 cut(s) 257, 867
BanI GGYRCC 1 cut(s) 1046
BanII GRGCYC 2 cut(s) 142, 814
BauI CACGAG 1 cut(s) 97
BbsI GAAGAC 1 cut(s) 1047
Bbv12I GWGCWC 2 cut(s) 142, 867
BccI CCATC 2 cut(s) 593, 1076
BceAI ACGGC 2 cut(s) 955, 1060
BciT130I CCWGG 1 cut(s) 505
BclI TGATCA 1 cut(s) 930
BcnI CCSGG 2 cut(s) 988, 989
BcoDI GTCTC 2 cut(s) 452, 785
BfaI CTAG 4 cut(s) 222, 822, 860, 899
BfmI CTRYAG 1 cut(s) 1009
BlpI GCTNAGC 1 cut(s) 855
Bme1390I CCNGG 3 cut(s) 505, 988, 989
BmeT110I CYCGRG 1 cut(s) 987
BmgBI CACGTC 1 cut(s) 628
BmgT120I GGNCC 2 cut(s) 643, 985
BmiI GGNNCC 2 cut(s) 419, 1048
BmrFI CCNGG 3 cut(s) 505, 988, 989
BmrI ACTGGG 1 cut(s) 239
BmsI GCATC 8 cut(s) 232, 377, 399, 748, 769, 871, 896, 945
BmuI ACTGGG 1 cut(s) 239
BoxI GACNNNNGTC 1 cut(s) 797
BpiI GAAGAC 1 cut(s) 1047
BpmI CTGGAG 1 cut(s) 765
Bpu10I CCTNAGC 2 cut(s) 395, 815
Bpu1102I GCTNAGC 1 cut(s) 855
BpuEI CTTGAG 1 cut(s) 307
BpuMI CCSGG 2 cut(s) 988, 989
Bsa29I ATCGAT 1 cut(s) 1093
BsaBI GATNNNNATC 1 cut(s) 1099
BsaHI GRCGYC 1 cut(s) 798
BsaJI CCNNGG 3 cut(s) 158, 981, 987
Bsc4I CCNNNNNNNGG 2 cut(s) 69, 987
Bse118I RCCGGY 1 cut(s) 637
Bse1I ACTGG 1 cut(s) 234
Bse8I GATNNNNATC 1 cut(s) 1099
BseBI CCWGG 1 cut(s) 505
BseCI ATCGAT 1 cut(s) 1093
BseDI CCNNGG 3 cut(s) 158, 981, 987
BseGI GGATG 4 cut(s) 390, 931, 960, 997
BseJI GATNNNNATC 1 cut(s) 1099
BseLI CCNNNNNNNGG 2 cut(s) 69, 987
BseMII CTCAG 1 cut(s) 846
BseNI ACTGG 1 cut(s) 234
BseRI GAGGAG 1 cut(s) 670
BseSI GKGCMC 2 cut(s) 257, 867
BseX3I CGGCCG 1 cut(s) 181
BseYI CCCAGC 1 cut(s) 258
BsgI GTGCAG 1 cut(s) 891
Bsh1236I CGCG 1 cut(s) 186
Bsh1285I CGRYCG 1 cut(s) 184
BshFI GGCC 3 cut(s) 183, 644, 986
BshNI GGYRCC 1 cut(s) 1046
BshVI ATCGAT 1 cut(s) 1093
BsiEI CGRYCG 1 cut(s) 184
BsiHKAI GWGCWC 2 cut(s) 142, 867
BsiHKCI CYCGRG 1 cut(s) 987
BsiSI CCGG 3 cut(s) 180, 638, 988
BslFI GGGAC 1 cut(s) 645
BslI CCNNNNNNNGG 2 cut(s) 69, 987
BsmAI GTCTC 2 cut(s) 452, 785
BsmFI GGGAC 1 cut(s) 645
BsnI GGCC 3 cut(s) 183, 644, 986
BsoBI CYCGRG 1 cut(s) 987
Bsp1286I GDGCHC 4 cut(s) 142, 257, 814, 867
Bsp143I GATC 6 cut(s) 343, 525, 930, 1090, 1094, 1100
Bsp1720I GCTNAGC 1 cut(s) 855
Bsp19I CCATGG 1 cut(s) 981
BspACI CCGC 2 cut(s) 184, 1006
BspANI GGCC 3 cut(s) 183, 644, 986
BspCNI CTCAG 1 cut(s) 847
BspDI ATCGAT 1 cut(s) 1093
BspFNI CGCG 1 cut(s) 186
BspLI GGNNCC 2 cut(s) 419, 1048
BspPI GGATC 4 cut(s) 338, 533, 1098, 1108
BspT107I GGYRCC 1 cut(s) 1046
BsrFI RCCGGY 1 cut(s) 637
BsrI ACTGG 1 cut(s) 234
BssAI RCCGGY 1 cut(s) 637
BssECI CCNNGG 3 cut(s) 158, 981, 987
BssMI GATC 6 cut(s) 343, 525, 930, 1090, 1094, 1100
BssNI GRCGYC 1 cut(s) 798
BssSI CACGAG 1 cut(s) 97
BssT1I CCWWGG 1 cut(s) 981
Bst2BI CACGAG 1 cut(s) 97
Bst2UI CCWGG 1 cut(s) 505
Bst4CI ACNGT 2 cut(s) 43, 709
BstACI GRCGYC 1 cut(s) 798
BstAPI GCANNNNNTGC 1 cut(s) 758
BstDEI CTNAG 4 cut(s) 395, 462, 815, 855
BstDSI CCRYGG 2 cut(s) 158, 981
BstEII GGTNACC 1 cut(s) 827
BstF5I GGATG 4 cut(s) 390, 931, 960, 997
BstFNI CGCG 1 cut(s) 186
BstKTI GATC 6 cut(s) 346, 528, 933, 1093, 1097, 1103
BstMAI GTCTC 2 cut(s) 452, 785
BstMBI GATC 6 cut(s) 343, 525, 930, 1090, 1094, 1100
BstMCI CGRYCG 1 cut(s) 184
BstMWI GCNNNNNNNGC 7 cut(s) 86, 89, 92, 137, 338, 396, 758
BstNI CCWGG 1 cut(s) 505
BstNSI RCATGY 1 cut(s) 844
BstPAI GACNNNNGTC 1 cut(s) 797
BstPI GGTNACC 1 cut(s) 827
BstSCI CCNGG 3 cut(s) 503, 986, 987
BstSFI CTRYAG 1 cut(s) 1009
BstSLI GKGCMC 2 cut(s) 257, 867
BstUI CGCG 1 cut(s) 186
BstV2I GAAGAC 1 cut(s) 1047
BstX2I RGATCY 1 cut(s) 343
BstXI CCANNNNNNTGG 1 cut(s) 17
BstYI RGATCY 1 cut(s) 343
BstZI CGGCCG 1 cut(s) 181
Bsu15I ATCGAT 1 cut(s) 1093
BsuRI GGCC 3 cut(s) 183, 644, 986
BsuTUI ATCGAT 1 cut(s) 1093
BtgI CCRYGG 2 cut(s) 158, 981
BtgZI GCGATG 1 cut(s) 876
BtrI CACGTC 1 cut(s) 628
BtsCI GGATG 4 cut(s) 390, 931, 960, 997
BtsIMutI CAGTG 1 cut(s) 705
CaiI CAGNNNCTG 1 cut(s) 761
Cfr10I RCCGGY 1 cut(s) 637
Cfr13I GGNCC 2 cut(s) 643, 985
Cfr9I CCCGGG 1 cut(s) 987
ClaI ATCGAT 1 cut(s) 1093
CseI GACGC 1 cut(s) 48
Csp6I GTAC 1 cut(s) 44
CviAII CATG 6 cut(s) 11, 487, 841, 934, 951, 982
CviQI GTAC 1 cut(s) 44
DdeI CTNAG 4 cut(s) 395, 462, 815, 855
DpnI GATC 6 cut(s) 345, 527, 932, 1092, 1096, 1102
DpnII GATC 6 cut(s) 343, 525, 930, 1090, 1094, 1100
EaeI YGGCCR 1 cut(s) 181
EagI CGGCCG 1 cut(s) 181
Ecl136II GAGCTC 1 cut(s) 140
EclXI CGGCCG 1 cut(s) 181
Eco130I CCWWGG 1 cut(s) 981
Eco24I GRGCYC 2 cut(s) 142, 814
Eco52I CGGCCG 1 cut(s) 181
Eco53kI GAGCTC 1 cut(s) 140
Eco57I CTGAAG 1 cut(s) 318
Eco88I CYCGRG 1 cut(s) 987
Eco91I GGTNACC 1 cut(s) 827
EcoICRI GAGCTC 1 cut(s) 140
EcoO65I GGTNACC 1 cut(s) 827
EcoRII CCWGG 1 cut(s) 503
EcoT14I CCWWGG 1 cut(s) 981
EcoT22I ATGCAT 1 cut(s) 392
EcoT38I GRGCYC 2 cut(s) 142, 814
ErhI CCWWGG 1 cut(s) 981
FaeI CATG 6 cut(s) 14, 490, 844, 937, 954, 985
FaqI GGGAC 1 cut(s) 645
FatI CATG 6 cut(s) 10, 486, 840, 933, 950, 981
FbaI TGATCA 1 cut(s) 930
FokI GGATG 4 cut(s) 377, 938, 967, 1004
FriOI GRGCYC 2 cut(s) 142, 814
FspBI CTAG 4 cut(s) 222, 822, 860, 899
GsaI CCCAGC 1 cut(s) 262
GsuI CTGGAG 1 cut(s) 765
HaeIII GGCC 3 cut(s) 183, 644, 986
HapII CCGG 3 cut(s) 180, 638, 988
HgaI GACGC 1 cut(s) 48
Hin1I GRCGYC 1 cut(s) 798
Hin1II CATG 6 cut(s) 14, 490, 844, 937, 954, 985
HinfI GANTC 7 cut(s) 73, 176, 204, 350, 611, 658, 731
HpaII CCGG 3 cut(s) 180, 638, 988
HphI GGTGA 1 cut(s) 821
Hpy166II GTNNAC 4 cut(s) 46, 865, 997, 1078
Hpy188I TCNGA 3 cut(s) 114, 337, 590
Hpy188III TCNNGA 4 cut(s) 347, 744, 822, 1098
Hpy8I GTNNAC 4 cut(s) 46, 865, 997, 1078
Hpy99I CGWCG 1 cut(s) 114
HpyAV CCTTC 4 cut(s) 8, 502, 911, 915
HpyCH4III ACNGT 2 cut(s) 43, 709
HpyCH4IV ACGT 2 cut(s) 627, 798
HpyCH4V TGCA 9 cut(s) 4, 245, 267, 390, 407, 621, 865, 887, 908
HpyF10VI GCNNNNNNNGC 7 cut(s) 86, 89, 92, 137, 338, 396, 758
HpyF3I CTNAG 4 cut(s) 395, 462, 815, 855
HpySE526I ACGT 2 cut(s) 627, 798
Hsp92I GRCGYC 1 cut(s) 798
Hsp92II CATG 6 cut(s) 14, 490, 844, 937, 954, 985
Ksp22I TGATCA 1 cut(s) 930
Kzo9I GATC 6 cut(s) 343, 525, 930, 1090, 1094, 1100
LmnI GCTCC 5 cut(s) 145, 417, 535, 746, 849
LweI GCATC 8 cut(s) 232, 377, 399, 748, 769, 871, 896, 945
MaeI CTAG 4 cut(s) 222, 822, 860, 899
MaeII ACGT 2 cut(s) 627, 798
MaeIII GTNAC 3 cut(s) 827, 901, 1025
MalI GATC 6 cut(s) 345, 527, 932, 1092, 1096, 1102
MboI GATC 6 cut(s) 343, 525, 930, 1090, 1094, 1100
MboII GAAGA 4 cut(s) 320, 478, 662, 1052
MflI RGATCY 1 cut(s) 343
MhlI GDGCHC 4 cut(s) 142, 257, 814, 867
MluCI AATT 3 cut(s) 234, 364, 377
MlyI GAGTC 2 cut(s) 667, 740
MmeI TCCRAC 2 cut(s) 92, 169
MnlI CCTC 9 cut(s) 47, 162, 516, 648, 722, 766, 818, 1030, 1081
Mph1103I ATGCAT 1 cut(s) 392
MroXI GAANNNNTTC 1 cut(s) 595
MseI TTAA 1 cut(s) 912
MslI CAYNNNNRTG 2 cut(s) 15, 626
MspA1I CMGCKG 1 cut(s) 262
MspI CCGG 3 cut(s) 180, 638, 988
MspR9I CCNGG 3 cut(s) 505, 988, 989
MvaI CCWGG 1 cut(s) 505
MvnI CGCG 1 cut(s) 186
MwoI GCNNNNNNNGC 7 cut(s) 86, 89, 92, 137, 338, 396, 758
NciI CCSGG 2 cut(s) 988, 989
NcoI CCATGG 1 cut(s) 981
NdeII GATC 6 cut(s) 343, 525, 930, 1090, 1094, 1100
NlaIII CATG 6 cut(s) 14, 490, 844, 937, 954, 985
NlaIV GGNNCC 2 cut(s) 419, 1048
NmuCI GTSAC 2 cut(s) 827, 901
NsiI ATGCAT 1 cut(s) 392
NspI RCATGY 1 cut(s) 844
OliI CACNNNNGTG 1 cut(s) 626
PcsI WCGNNNNNNNCGW 1 cut(s) 128
PdmI GAANNNNTTC 1 cut(s) 595
PfeI GAWTC 5 cut(s) 73, 176, 204, 350, 611
PleI GAGTC 2 cut(s) 666, 739
PpsI GAGTC 2 cut(s) 666, 739
PshAI GACNNNNGTC 1 cut(s) 797
Psp124BI GAGCTC 1 cut(s) 142
Psp6I CCWGG 1 cut(s) 503
PspEI GGTNACC 1 cut(s) 827
PspFI CCCAGC 1 cut(s) 258
PspGI CCWGG 1 cut(s) 503
PspN4I GGNNCC 2 cut(s) 419, 1048
PspPI GGNCC 2 cut(s) 643, 985
PstNI CAGNNNCTG 1 cut(s) 761
PsuI RGATCY 1 cut(s) 343
PvuII CAGCTG 1 cut(s) 262
RsaI GTAC 1 cut(s) 45
RsaNI GTAC 1 cut(s) 44
RseI CAYNNNNRTG 2 cut(s) 15, 626
SacI GAGCTC 1 cut(s) 142
SaqAI TTAA 1 cut(s) 912
Sau3AI GATC 6 cut(s) 343, 525, 930, 1090, 1094, 1100
Sau96I GGNCC 2 cut(s) 643, 985
SchI GAGTC 2 cut(s) 667, 740
ScrFI CCNGG 3 cut(s) 505, 988, 989
SduI GDGCHC 4 cut(s) 142, 257, 814, 867
SfaNI GCATC 8 cut(s) 232, 377, 399, 748, 769, 871, 896, 945
SfcI CTRYAG 1 cut(s) 1009
SmaI CCCGGG 1 cut(s) 989
SmiMI CAYNNNNRTG 2 cut(s) 15, 626
SmlI CTYRAG 1 cut(s) 286
SmoI CTYRAG 1 cut(s) 286
Sse9I AATT 3 cut(s) 234, 364, 377
SsiI CCGC 2 cut(s) 184, 1006
SspI AATATT 1 cut(s) 326
SspMI CTAG 4 cut(s) 222, 822, 860, 899
SstI GAGCTC 1 cut(s) 142
StyD4I CCNGG 3 cut(s) 503, 986, 987
StyI CCWWGG 1 cut(s) 981
TaaI ACNGT 2 cut(s) 43, 709
TaiI ACGT 2 cut(s) 630, 801
TaqI TCGA 2 cut(s) 198, 1093
TasI AATT 3 cut(s) 234, 364, 377
TatI WGTACW 1 cut(s) 43
TauI GCSGC 2 cut(s) 186, 1008
TfiI GAWTC 5 cut(s) 73, 176, 204, 350, 611
Tru1I TTAA 1 cut(s) 912
Tru9I TTAA 1 cut(s) 912
TscAI CASTG 1 cut(s) 712
TseFI GTSAC 2 cut(s) 827, 901
Tsp45I GTSAC 2 cut(s) 827, 901
TspDTI ATGAA 7 cut(s) 27, 390, 401, 588, 593, 647, 930
TspGWI ACGGA 3 cut(s) 175, 188, 638
TspMI CCCGGG 1 cut(s) 987
TspRI CASTG 1 cut(s) 712
VneI GTGCAC 1 cut(s) 863
XapI RAATTY 3 cut(s) 234, 364, 377
XbaI TCTAGA 1 cut(s) 821
XceI RCATGY 1 cut(s) 844
XmaI CCCGGG 1 cut(s) 987
XmnI GAANNNNTTC 1 cut(s) 595
XspI CTAG 4 cut(s) 222, 822, 860, 899
ZraI GACGTC 1 cut(s) 799
Zsp2I ATGCAT 1 cut(s) 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.