pycom13g24270

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0019313
Physical Location & Seq
Forward (+)
21468107 .. 21472716
4610 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3261 bp
ATGCAAAATATATTGCCATATAGAAGCTTACAACATTGTTGTTTGGATGGGAAAGACACTAATGGGGTGATAGCTCAAGCCAGGGAATCAAGGTCTCATCAAGGTCCGAACTCAAATGAGAGACTGAACCACATGATTGAGAATCATGCCTACTACTCAGCCATTTATGAAATGACTACAGAAGAGGTATCATCACTGATGACTAAGCTGATTGGCTCTAGTGCAAGTGGGAGATTGTTGGAAATGTGCCCTAAAACCAATCATATGATGATACTTTATGGACATTTCACATGCTTAAACGATAAGTCCAAGGAATATGTGATTGGAAGAATGCGATCTAAAGAAGTTAGATTCATGAGACCATTCTTTCATCAGTACGTGCTATGTCTTCTCTCAGGGAGAGTGACTAGTCTCGAGTCATTGGTGTGTGTGACATCAAGACAAGCATGTAGGTGCTCAATAGAGAATGAGTTCACTGAACACGATCAACGAGGGGTTCTCATATTCATGTCACATGAGAACACATGCTATCTTGTGGTTAGGTCCTTAATCTTTGATTATGTCAAAGTCACTCCATCAGGAGGGTGTCCACGGCGCAACAAGGGATCTCTAACCTTCAAACTGTTTGAGGGAGAATACTCTATGATATGTTTTAGAATCTCTGGCCAGAGTATGAATGAGATGTTGGAATGCGTTCCAAATCACATTCAAGGTAATCATATAAGCACAAGACTCGCATTGGATAAGTATTGTATTAGAGAAAGACCGTATTGCATTTGTAATCCTAAACTGAATAGGTTCTCCACCTCTTCTGATTACCCTAAAAGTGTATTATCACTAACGGGAGAATTGAAAGTAAGTTTCAATTCACAATCGATTAGAAAGAGTTTTAATCGCCCACTGCCTCTAAATGGTTTAATTATTTATCTAAGGCTAGGATTAATTAATATGTTAATTAATCAAACGAATAAGTTCGTTAAAGACCTCGGGATAGTTTTGGACCTTAAGGCCCAATGGGCTTCGAACGTCAATCACAAAGGCCAAAATAGCCCAATAAATCCCCTTAAATTCGGCCATTTAGAGGAGGGTAGTGAACTTGGCTTAATTAGCCATTTCATCCTAAGGGTTTTTCTAAGGAAAAAAGATGAGAACAAACTCTCCTTTCTCTCTAAAATGGTCACTCATTTCTTCTCCAATCTTACCTTGGTGTGGAGACTTAGAGGTTCTCAATTTTGGGAACTTGGAGAAACCTTTTCATCCATCCAAATCCATGCATCTAAGATGCAAGGAATGAAGGCCCTCTCTTTGGTTGAGTCTTGGTTCACCTATCTACTAGGCTTTGAAGTTCATGGGTTAATGCATGTTGTTGATGTTGCTCAAATGAACTTGTTTTTCACAAATATTCCTTCAGATGAACAACAAGTGGATGAGTTTCTCACAAAGGGTTTTATTCGGGAAAGCTTGTGTGGTTCCTGCCTTAGTCGAGCGATCAACAAAATCACAATTAAGTATCGATTTTCGATGCCAAGATTTAATGATCTTCTTTACCAACTGTGTGGTGCCATCGTACTCAGTCAAATTGATTTGAAAAGTGGGTATCATCAGATACGCATTAGAGAGGATGAATCGTTGGTCATGCCATTCGGGTTGACTAATGCTCCGAGTACTTTTATGCGAGTTATGAATCAGGTACTTCAGCCTTTTATTGGAACTTATGTGGTGGTATATTTTGATGACATTCTTGTCTACAGCACATGTCATGCCTTACATAAATTTTATGCAAATTTAAAGAAGTGTACATATATGACTGATCACATGCTGTTCCTTCGATACGTTGTATCTAAAAATGGAATACCTATGGATAATAAACAGGTTGAAGCAGTCACCACATGGCCTACGCCACAATCCATACATGATGTACAAAGTTTTCATGGATTTTATGGCGCTTTGATCACCGATTGTATGAATGGTGGAAAGTTCTCTTGGAATGGAGAAGCAGCAGTTGCCTTCAATCAAATCAAGAAAAAGTTGAGTTTGGCTTCGTTGCTTGTGTTACCTGACTTCTCAAAAGTTTTTGAGCTATCTTGCGATGCATCCAAGATTAGGATTGGAGCTATTCTAAGTCAAGAAGGGAAGCTTATTGCTTATTTCAATGAAAAACTGAGTAGTTCAAGATTGAATTATTCCACATATGACTTAGAATTCAATGCTATTTTTGTTATGTTCTCTGATCATGACTCTCTAAAGCATATAAACAGTCAGGAAAAATTGTCAACGAGGCATGCTAAGTGGGCAGCATACTTACAAGAATTTATATTTGTCTTAAAGCACAAAGTCAGTGTGCAAAATCAAGTTGCAGATGCCTTAAGTCGAAGGAGATATTTACTTGGTTCGATTAGAATTCAAGTATTAGGGTTTGACTCTTCGAAGGATTTATTGAAAATTGATCCTTTCTTCTCTACAATTGTCGAAAATGTTGAGGATGGAACTAGAACATATTATGTTTTGCATGAAGGGTTTCTCTTTAAAGGAAATCAACTTTTTACTAATGAAGGCCACGTTGGAAGGGACAAGACCTTTGAGCTTGTTTCTTTCTCTTATATTTGGCCTACCATGCGGAAAGAAGTATATCATTTTGTGGAACGATGCCGAATCTATCAAGTTTCCAAGGGCAAGGCGACCAACACTGGATTGTACATGTCATTACCTATTCCATCACAACCATGGGTGGATATAAGCATGGATTCTGTTTTGGATTTTGATAGGATTAAAAGAACACCACAAAGTAGCACACAAATAAAAATAAGGATCTATCCCGTAGAAGATAGTTTTATCCAACTACTTAAAATGTCACAAAAACTGGGTTACTACTTATCTAAGTCTATGAAATTTTATATGAAAACACTAGACACACAGAGCTACACTCACACAAATTTTTGGGACTTTTGTAAAAAACGAGTTAGGGGAACTGTTATCCACCAACGAATAATCATGCAAACATGTTTTGTTTCATTCAAATTCCTTTTATTTCCGGATGAAGATGCTCAAGTTGCCTTAATGTTAGAACTCAACCTATTACTCTTTCTTATGTACTGGAATGGCGTGGTCATAGATTTAACAAGCATCGTTAGTACTAGCGTTGTCTTACTTATCCTAGAAATTGATTCACATGTTAATATCGACAAGGGCAAGCACACACATATTCATAGCATTAGAATCCTAGATATGCTTACTAAGTATGCATCTGTAGAAAACACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 Retrotrans_gag rve zf-CCHC zf-H2C2

Protein Analysis

1087

Amino Acids

125.05

Weight (kDa)

8.99

Isoelectric Point (pI)

36.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 543 - 586 1.1e-09 Reverse transcriptase (RNA-dependent DNA polymerase)
RT_RNaseH_2 PF17919 662 - 743 2.2e-18 RNase H-like domain found in reverse transcriptase
RT_RNaseH PF17917 687 - 771 2.5e-13 RNase H-like domain found in reverse transcriptase
Integrase_H2C2 PF17921 852 - 890 5.6e-09 Integrase zinc binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1742
AccB1I GGYRCC 1 cut(s) 1559
AccI GTMKAC 1 cut(s) 1746
AccIII TCCGGA 1 cut(s) 3031
AciI CCGC 1 cut(s) 2617
AclWI GGATC 3 cut(s) 613, 2441, 2816
AcoI YGGCCR 2 cut(s) 664, 1072
AcsI RAATTY 9 cut(s) 1067, 1772, 1783, 2201, 2309, 2400, 2888, 2932, 3017
AcuI CTGAAG 2 cut(s) 1392, 1679
AfaI GTAC 9 cut(s) 377, 1569, 1666, 1692, 1798, 1920, 2696, 3092, 3133
AfiI CCNNNNNNNGG 7 cut(s) 581, 911, 1081, 1209, 1306, 1706, 2103
AflII CTTAAG 2 cut(s) 1004, 2365
AflIII ACRYGT 4 cut(s) 1754, 2697, 2999, 3169
AhlI ACTAGT 1 cut(s) 407
AjnI CCWGG 1 cut(s) 80
AjuI GAANNNNNNNTTGG 8 cut(s) 306, 338, 668, 700, 1966, 1998, 2544, 2576
AloI GAACNNNNNNTCC 2 cut(s) 1142, 1174
AluBI AGCT 9 cut(s) 27, 74, 208, 1461, 2080, 2114, 2137, 2584, 2919
AluI AGCT 9 cut(s) 27, 74, 208, 1461, 2080, 2114, 2137, 2584, 2919
Alw21I GWGCWC 1 cut(s) 458
Alw26I GTCTC 5 cut(s) 99, 115, 352, 416, 1207
AlwI GGATC 3 cut(s) 613, 2441, 2816
Ama87I CYCGRG 2 cut(s) 413, 986
Aor13HI TCCGGA 1 cut(s) 3031
AoxI GGCC 8 cut(s) 664, 1008, 1039, 1072, 1296, 1892, 2554, 2606
ApeKI GCWGC 2 cut(s) 1997, 2294
ApoI RAATTY 9 cut(s) 1067, 1772, 1783, 2201, 2309, 2400, 2888, 2932, 3017
ArsI GACNNNNNNTTYG 2 cut(s) 2561, 2593
AseI ATTAAT 3 cut(s) 941, 945, 957
Asp700I GAANNNNTTC 5 cut(s) 470, 693, 797, 971, 2517
AspLEI GCGC 2 cut(s) 597, 1946
AspS9I GGNCC 5 cut(s) 104, 543, 1000, 1009, 1297
AsuHPI GGTGA 4 cut(s) 79, 1315, 1876, 1945
AsuII TTCGAA 2 cut(s) 1022, 2426
AvaI CYCGRG 2 cut(s) 413, 986
AvaII GGWCC 3 cut(s) 104, 543, 1000
AxyI CCTNAGG 1 cut(s) 1121
BaeGI GKGCMC 1 cut(s) 251
BalI TGGCCA 1 cut(s) 666
BanI GGYRCC 1 cut(s) 1559
BbsI GAAGAC 1 cut(s) 380
Bbv12I GWGCWC 1 cut(s) 458
BbvI GCAGC 2 cut(s) 2009, 2306
BccI CCATC 6 cut(s) 41, 583, 1268, 1571, 2477, 2722
BceAI ACGGC 1 cut(s) 608
BcgI CGANNNNNNTGC 2 cut(s) 715, 749
BciT130I CCWGG 1 cut(s) 82
BclI TGATCA 3 cut(s) 1810, 1950, 2230
BcoDI GTCTC 5 cut(s) 99, 115, 352, 416, 1207
BcuI ACTAGT 1 cut(s) 407
BfaI CTAG 9 cut(s) 219, 408, 935, 1334, 2490, 2906, 3135, 3155, 3221
BfmI CTRYAG 3 cut(s) 177, 1747, 3246
BfoI RGCGCY 1 cut(s) 1947
BfrI CTTAAG 2 cut(s) 1004, 2365
BglI GCCNNNNNGGC 1 cut(s) 1016
BisI GCNGC 2 cut(s) 1998, 2295
BlsI GCNGC 2 cut(s) 1999, 2296
BmcAI AGTACT 2 cut(s) 1666, 3133
Bme1390I CCNGG 1 cut(s) 82
Bme18I GGWCC 3 cut(s) 104, 543, 1000
BmeT110I CYCGRG 2 cut(s) 413, 986
BmgT120I GGNCC 5 cut(s) 104, 543, 1000, 1009, 1297
BmiI GGNNCC 2 cut(s) 1471, 1561
BmrFI CCNGG 1 cut(s) 82
BmrI ACTGGG 1 cut(s) 2870
BmuI ACTGGG 1 cut(s) 2870
BpiI GAAGAC 1 cut(s) 380
BplI GAGNNNNNCTC 4 cut(s) 483, 515, 2908, 2940
Bpu14I TTCGAA 2 cut(s) 1022, 2426
BpuEI CTTGAG 2 cut(s) 60, 3030
Bsa29I ATCGAT 2 cut(s) 875, 1513
BsaAI YACGTR 1 cut(s) 379
BsaI GGTCTC 2 cut(s) 99, 352
BsaJI CCNNGG 7 cut(s) 81, 309, 590, 985, 1203, 2667, 2723
BsaWI WCCGGW 1 cut(s) 3031
BsaXI ACNNNNNCTCC 4 cut(s) 1142, 1172, 1642, 1672
Bsc4I CCNNNNNNNGG 7 cut(s) 581, 911, 1081, 1209, 1306, 1706, 2103
Bse1I ACTGG 3 cut(s) 2692, 2865, 3098
Bse21I CCTNAGG 1 cut(s) 1121
BseAI TCCGGA 1 cut(s) 3031
BseBI CCWGG 1 cut(s) 82
BseCI ATCGAT 2 cut(s) 875, 1513
BseDI CCNNGG 7 cut(s) 81, 309, 590, 985, 1203, 2667, 2723
BseGI GGATG 9 cut(s) 52, 1116, 1256, 1260, 1432, 1627, 2093, 2488, 3040
BseLI CCNNNNNNNGG 7 cut(s) 581, 911, 1081, 1209, 1306, 1706, 2103
BseMII CTCAG 4 cut(s) 171, 408, 1585, 2153
BseNI ACTGG 3 cut(s) 2692, 2865, 3098
BseRI GAGGAG 1 cut(s) 1097
BseSI GKGCMC 1 cut(s) 251
BseXI GCAGC 2 cut(s) 2009, 2306
BshFI GGCC 8 cut(s) 666, 1010, 1041, 1074, 1298, 1894, 2556, 2608
BshNI GGYRCC 1 cut(s) 1559
BshVI ATCGAT 2 cut(s) 875, 1513
BsiHKAI GWGCWC 1 cut(s) 458
BsiHKCI CYCGRG 2 cut(s) 413, 986
BsiSI CCGG 1 cut(s) 3032
BslFI GGGAC 2 cut(s) 2582, 2954
BslI CCNNNNNNNGG 7 cut(s) 581, 911, 1081, 1209, 1306, 1706, 2103
BsmAI GTCTC 5 cut(s) 99, 115, 352, 416, 1207
BsmFI GGGAC 2 cut(s) 2582, 2954
BsmI GAATGC 2 cut(s) 336, 695
BsnI GGCC 8 cut(s) 666, 1010, 1041, 1074, 1298, 1894, 2556, 2608
Bso31I GGTCTC 2 cut(s) 99, 352
BsoBI CYCGRG 2 cut(s) 413, 986
Bsp119I TTCGAA 2 cut(s) 1022, 2426
Bsp1286I GDGCHC 2 cut(s) 251, 458
Bsp13I TCCGGA 1 cut(s) 3031
Bsp1407I TGTACA 3 cut(s) 1796, 1918, 2694
Bsp19I CCATGG 1 cut(s) 2723
BspACI CCGC 1 cut(s) 2617
BspANI GGCC 8 cut(s) 666, 1010, 1041, 1074, 1298, 1894, 2556, 2608
BspCNI CTCAG 4 cut(s) 170, 407, 1584, 2154
BspDI ATCGAT 2 cut(s) 875, 1513
BspEI TCCGGA 1 cut(s) 3031
BspHI TCATGA 2 cut(s) 354, 2233
BspLI GGNNCC 2 cut(s) 1471, 1561
BspPI GGATC 3 cut(s) 613, 2441, 2816
BspT104I TTCGAA 2 cut(s) 1022, 2426
BspT107I GGYRCC 1 cut(s) 1559
BspTI CTTAAG 2 cut(s) 1004, 2365
BspTNI GGTCTC 2 cut(s) 99, 352
BsrGI TGTACA 3 cut(s) 1796, 1918, 2694
BsrI ACTGG 3 cut(s) 2692, 2865, 3098
BssECI CCNNGG 7 cut(s) 81, 309, 590, 985, 1203, 2667, 2723
BssT1I CCWWGG 4 cut(s) 309, 1203, 2667, 2723
Bst2UI CCWGG 1 cut(s) 82
Bst4CI ACNGT 5 cut(s) 624, 768, 1554, 2258, 2971
Bst6I CTCTTC 3 cut(s) 177, 814, 2428
BstAFI CTTAAG 2 cut(s) 1004, 2365
BstAPI GCANNNNNTGC 1 cut(s) 2003
BstAUI TGTACA 3 cut(s) 1796, 1918, 2694
BstBAI YACGTR 1 cut(s) 379
BstBI TTCGAA 2 cut(s) 1022, 2426
BstC8I GCNNGC 2 cut(s) 2283, 3191
BstDSI CCRYGG 2 cut(s) 590, 2723
BstF5I GGATG 9 cut(s) 52, 1116, 1256, 1260, 1432, 1627, 2093, 2488, 3040
BstH2I RGCGCY 1 cut(s) 1947
BstHHI GCGC 2 cut(s) 597, 1946
BstMAI GTCTC 5 cut(s) 99, 115, 352, 416, 1207
BstMWI GCNNNNNNNGC 7 cut(s) 1016, 1047, 1107, 2003, 2291, 2614, 3050
BstNI CCWGG 1 cut(s) 82
BstSCI CCNGG 1 cut(s) 80
BstSFI CTRYAG 3 cut(s) 177, 1747, 3246
BstSLI GKGCMC 1 cut(s) 251
BstV1I GCAGC 2 cut(s) 2009, 2306
BstV2I GAAGAC 1 cut(s) 380
BstX2I RGATCY 2 cut(s) 605, 2808
BstXI CCANNNNNNTGG 1 cut(s) 1556
BstYI RGATCY 2 cut(s) 605, 2808
Bsu15I ATCGAT 2 cut(s) 875, 1513
Bsu36I CCTNAGG 1 cut(s) 1121
BsuRI GGCC 8 cut(s) 666, 1010, 1041, 1074, 1298, 1894, 2556, 2608
BsuTUI ATCGAT 2 cut(s) 875, 1513
BtgI CCRYGG 2 cut(s) 590, 2723
BtgZI GCGATG 1 cut(s) 2103
BtsCI GGATG 9 cut(s) 52, 1116, 1256, 1260, 1432, 1627, 2093, 2488, 3040
BtsI GCAGTG 1 cut(s) 899
BtsIMutI CAGTG 5 cut(s) 194, 474, 899, 2344, 2685
Cac8I GCNNGC 2 cut(s) 2283, 3191
CciI TCATGA 2 cut(s) 354, 2233
CfoI GCGC 2 cut(s) 597, 1946
Cfr13I GGNCC 5 cut(s) 104, 543, 1000, 1009, 1297
ClaI ATCGAT 2 cut(s) 875, 1513
Csp6I GTAC 9 cut(s) 376, 1568, 1665, 1691, 1797, 1919, 2695, 3091, 3132
CviQI GTAC 9 cut(s) 376, 1568, 1665, 1691, 1797, 1919, 2695, 3091, 3132
DraI TTTAAA 2 cut(s) 1788, 2527
DrdI GACNNNNNNGTC 1 cut(s) 1742
DseDI GACNNNNNNGTC 1 cut(s) 1742
EaeI YGGCCR 2 cut(s) 664, 1072
Eam1104I CTCTTC 3 cut(s) 177, 814, 2428
EarI CTCTTC 3 cut(s) 177, 814, 2428
Eco130I CCWWGG 4 cut(s) 309, 1203, 2667, 2723
Eco31I GGTCTC 2 cut(s) 99, 352
Eco47I GGWCC 3 cut(s) 104, 543, 1000
Eco57I CTGAAG 2 cut(s) 1392, 1679
Eco81I CCTNAGG 1 cut(s) 1121
Eco88I CYCGRG 2 cut(s) 413, 986
EcoO109I RGGNCCY 2 cut(s) 543, 1297
EcoRI GAATTC 2 cut(s) 2201, 2400
EcoRII CCWGG 1 cut(s) 80
EcoT14I CCWWGG 4 cut(s) 309, 1203, 2667, 2723
EcoT22I ATGCAT 4 cut(s) 1276, 1362, 2095, 3244
ErhI CCWWGG 4 cut(s) 309, 1203, 2667, 2723
FalI AAGNNNNNCTT 2 cut(s) 2121, 2153
FaqI GGGAC 2 cut(s) 2582, 2954
FauNDI CATATG 2 cut(s) 264, 2191
FbaI TGATCA 3 cut(s) 1810, 1950, 2230
FblI GTMKAC 1 cut(s) 1746
Fnu4HI GCNGC 2 cut(s) 1998, 2295
FokI GGATG 9 cut(s) 59, 1103, 1243, 1247, 1439, 1634, 2080, 2495, 3047
Fsp4HI GCNGC 2 cut(s) 1998, 2295
FspBI CTAG 9 cut(s) 219, 408, 935, 1334, 2490, 2906, 3135, 3155, 3221
GlaI GCGC 2 cut(s) 596, 1945
GluI GCNGC 2 cut(s) 1998, 2295
HaeII RGCGCY 1 cut(s) 1947
HaeIII GGCC 8 cut(s) 666, 1010, 1041, 1074, 1298, 1894, 2556, 2608
HapII CCGG 1 cut(s) 3032
HhaI GCGC 2 cut(s) 597, 1946
Hin6I GCGC 2 cut(s) 595, 1944
HinP1I GCGC 2 cut(s) 595, 1944
HincII GTYRAC 2 cut(s) 1650, 2274
HindII GTYRAC 2 cut(s) 1650, 2274
HindIII AAGCTT 3 cut(s) 25, 1459, 2135
HpaII CCGG 1 cut(s) 3032
HphI GGTGA 4 cut(s) 79, 1315, 1876, 1945
Hpy166II GTNNAC 8 cut(s) 474, 590, 1094, 1323, 1650, 1747, 1797, 2274
Hpy188I TCNGA 6 cut(s) 108, 814, 1411, 1605, 1662, 2230
Hpy8I GTNNAC 8 cut(s) 474, 590, 1094, 1323, 1650, 1747, 1797, 2274
HpyCH4III ACNGT 5 cut(s) 624, 768, 1554, 2258, 2971
HpyCH4IV ACGT 4 cut(s) 378, 1026, 1833, 2559
HpyF10VI GCNNNNNNNGC 7 cut(s) 1016, 1047, 1107, 2003, 2291, 2614, 3050
HpySE526I ACGT 4 cut(s) 378, 1026, 1833, 2559
HspAI GCGC 2 cut(s) 595, 1944
Kpn2I TCCGGA 1 cut(s) 3031
Ksp22I TGATCA 3 cut(s) 1810, 1950, 2230
LmnI GCTCC 2 cut(s) 1663, 2111
Lsp1109I GCAGC 2 cut(s) 2009, 2306
MaeI CTAG 9 cut(s) 219, 408, 935, 1334, 2490, 2906, 3135, 3155, 3221
MaeII ACGT 4 cut(s) 378, 1026, 1833, 2559
MaeIII GTNAC 9 cut(s) 403, 430, 510, 568, 1177, 1882, 2052, 2850, 2864
MfeI CAATTG 1 cut(s) 2463
MflI RGATCY 2 cut(s) 605, 2808
MhlI GDGCHC 2 cut(s) 251, 458
MlsI TGGCCA 1 cut(s) 666
MluNI TGGCCA 1 cut(s) 666
MlyI GAGTC 5 cut(s) 425, 726, 1322, 2231, 2414
MmeI TCCRAC 4 cut(s) 219, 666, 2542, 2860
Mox20I TGGCCA 1 cut(s) 666
Mph1103I ATGCAT 4 cut(s) 1276, 1362, 2095, 3244
MroI TCCGGA 1 cut(s) 3031
MroXI GAANNNNTTC 5 cut(s) 470, 693, 797, 971, 2517
MscI TGGCCA 1 cut(s) 666
MslI CAYNNNNRTG 4 cut(s) 424, 451, 506, 2722
Msp20I TGGCCA 1 cut(s) 666
MspCI CTTAAG 2 cut(s) 1004, 2365
MspI CCGG 1 cut(s) 3032
MspR9I CCNGG 1 cut(s) 82
MunI CAATTG 1 cut(s) 2463
Mva1269I GAATGC 2 cut(s) 336, 695
MvaI CCWGG 1 cut(s) 82
MwoI GCNNNNNNNGC 7 cut(s) 1016, 1047, 1107, 2003, 2291, 2614, 3050
NcoI CCATGG 1 cut(s) 2723
NdeI CATATG 2 cut(s) 264, 2191
NlaIV GGNNCC 2 cut(s) 1471, 1561
NmuCI GTSAC 7 cut(s) 403, 430, 510, 568, 1177, 1882, 2850
NsiI ATGCAT 4 cut(s) 1276, 1362, 2095, 3244
NspV TTCGAA 2 cut(s) 1022, 2426
PacI TTAATTAA 2 cut(s) 945, 957
PaeI GCATGC 1 cut(s) 2285
PaeR7I CTCGAG 1 cut(s) 413
PagI TCATGA 2 cut(s) 354, 2233
PciI ACATGT 4 cut(s) 1754, 2697, 2999, 3169
PctI GAATGC 2 cut(s) 336, 695
PdmI GAANNNNTTC 5 cut(s) 470, 693, 797, 971, 2517
PkrI GCNGC 2 cut(s) 1999, 2296
PleI GAGTC 5 cut(s) 424, 726, 1321, 2231, 2414
PpsI GAGTC 5 cut(s) 424, 726, 1321, 2231, 2414
Ppu21I YACGTR 1 cut(s) 379
PpuMI RGGWCCY 1 cut(s) 543
PscI ACATGT 4 cut(s) 1754, 2697, 2999, 3169
PshBI ATTAAT 3 cut(s) 941, 945, 957
Psp5II RGGWCCY 1 cut(s) 543
Psp6I CCWGG 1 cut(s) 80
PspGI CCWGG 1 cut(s) 80
PspN4I GGNNCC 2 cut(s) 1471, 1561
PspPI GGNCC 5 cut(s) 104, 543, 1000, 1009, 1297
PspPPI RGGWCCY 1 cut(s) 543
PsuI RGATCY 2 cut(s) 605, 2808
RsaI GTAC 9 cut(s) 377, 1569, 1666, 1692, 1798, 1920, 2696, 3092, 3133
RsaNI GTAC 9 cut(s) 376, 1568, 1665, 1691, 1797, 1919, 2695, 3091, 3132
RseI CAYNNNNRTG 4 cut(s) 424, 451, 506, 2722
SatI GCNGC 2 cut(s) 1998, 2295
Sau96I GGNCC 5 cut(s) 104, 543, 1000, 1009, 1297
ScaI AGTACT 2 cut(s) 1666, 3133
SchI GAGTC 5 cut(s) 425, 726, 1322, 2231, 2414
ScrFI CCNGG 1 cut(s) 82
SduI GDGCHC 2 cut(s) 251, 458
SfcI CTRYAG 3 cut(s) 177, 1747, 3246
Sfr274I CTCGAG 1 cut(s) 413
SfuI TTCGAA 2 cut(s) 1022, 2426
SinI GGWCC 3 cut(s) 104, 543, 1000
SlaI CTCGAG 1 cut(s) 413
SmiMI CAYNNNNRTG 4 cut(s) 424, 451, 506, 2722
SmlI CTYRAG 5 cut(s) 75, 413, 1004, 2365, 3045
SmoI CTYRAG 5 cut(s) 75, 413, 1004, 2365, 3045
SpeI ACTAGT 1 cut(s) 407
SphI GCATGC 1 cut(s) 2285
SsiI CCGC 1 cut(s) 2617
SspI AATATT 1 cut(s) 1402
SspMI CTAG 9 cut(s) 219, 408, 935, 1334, 2490, 2906, 3135, 3155, 3221
StyD4I CCNGG 1 cut(s) 80
StyI CCWWGG 4 cut(s) 309, 1203, 2667, 2723
TaaI ACNGT 5 cut(s) 624, 768, 1554, 2258, 2971
TaiI ACGT 4 cut(s) 381, 1029, 1836, 2562
TatI WGTACW 6 cut(s) 1664, 1796, 1918, 2694, 3090, 3131
TscAI CASTG 5 cut(s) 201, 481, 906, 2344, 2692
TseFI GTSAC 7 cut(s) 403, 430, 510, 568, 1177, 1882, 2850
TseI GCWGC 2 cut(s) 1997, 2294
Tsp45I GTSAC 7 cut(s) 403, 430, 510, 568, 1177, 1882, 2850
TspRI CASTG 5 cut(s) 201, 481, 906, 2344, 2692
Vha464I CTTAAG 2 cut(s) 1004, 2365
VpaK11BI GGWCC 3 cut(s) 104, 543, 1000
VspI ATTAAT 3 cut(s) 941, 945, 957
XapI RAATTY 9 cut(s) 1067, 1772, 1783, 2201, 2309, 2400, 2888, 2932, 3017
XcmI CCANNNNNNNNNTGG 2 cut(s) 1201, 2721
XhoI CTCGAG 1 cut(s) 413
XmiI GTMKAC 1 cut(s) 1746
XmnI GAANNNNTTC 5 cut(s) 470, 693, 797, 971, 2517
XspI CTAG 9 cut(s) 219, 408, 935, 1334, 2490, 2906, 3135, 3155, 3221
ZrmI AGTACT 2 cut(s) 1666, 3133
Zsp2I ATGCAT 4 cut(s) 1276, 1362, 2095, 3244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.