pycom13g24560

Preprotein translocase subunit SCY1

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
Physical Location & Seq
Forward (+)
21648345 .. 21655424
7080 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1674 bp
ATGTTGATAACGCTCAGAGGATCCTTTTCTTCTTCCCCGCTTTGCTTCAACAAACCCCATAGCCGTAGCCATAGCCATAGCCATAACCACAGCTTCTCTCATCTTACTCCCTCACCCTCTCCAGCACTGAAAAGATCGATCTGCAAACCCAGCTTCTCCGTTCAGAGAAAACCCAACTCCACCACTACATCTTGGAAGCTTGGCGTTCTCTCCAGCCGCTCAGAAGCCTCAGTTTTTGATCCCTTGGGTATAAATTCAGATGTCAATTCTGGTGTAAATGCTACCTGGGAAAGTTTCGTAGGCATATTATCACAAACGTTTGAGAGTGCCTCAAGTTCGAAGAAGGAGAAACCAACTTCAGCTAGAGGCTTGGCAGCTGCAATAGAGGACAGTTCCATTGATTTTGGAGACTTCTTTAAAGGTCCATTACCCGGAAAGTTCCTCAAGCTGTTGGCATATCTGGCCTTGTCTCGACTTGGGATATATATACCTCTTGGTGGGGTTAACCGTGAGGCTTTTGTTGGAAATTTGGACGGTAATAGTTTATTGAGCACCTTGGATTCTTTCTCTGGAGGAGGCATTGGAAGACTTGGGATATGTTCACTTGGTATTGTTCCATTCATCAATGCCTCGATTGTCTTCCAGCTTCTTGCACAAATTTATCCCAAGTTGCAGGATCTTCAGAAAAGAGAAGGAGAAGCAGGAAGAAAGAAAATTGTTCGGTATACTCAATATGCTTCAGTTGGGTTTGCCATAGTCCAGGCAATTGGCCAAGTATTCTACCTTCGTCCATATGTCAATGACTTCAGTACAGAGTGGGTTATCTCTTCTGTTACCTTATTGACACTTGGCTCAGTATTGACAACTTACATTGGCGAACGAATCACAGACCTAAAACTCGGAAATGGCACATCTCTTTTGATATTCACAAGCATCATCTCGTACTTTCCAGCATCCGTCGGTAGGACTGTTGCACAGGCATTTCAGGATGGTAACTATGTTGGACTTGCCACCATAGTTGTCTCCTTCTTTGTATTGGTCCTTGGTATTGTATATGTTCAGGAAGCAGAAAGGAAAATTCCACTTAACTATGCCTCAAGGTATAACACCAACAGAGGTGGAGGGCTTCAAAGATCTGCTTACCTACCCTTTAAGGTGAATAGTTCTGGAGTTATGCCAATAATATTTTCTACATCATCATTAGCTCTTCCTGGTACTATAGCACGCTTCACCGGTTTAGCCGCATTGAAGAACGCTGCAGTGGCTTTAAATCCAGGGGGTTCTTTCTATCTCCCCACCAACATCCTTTTAATAGCCTTCTTCAACTACTACTACACTTTCCTACAGTTGGATCCTGATGATGTAAGTGAACAGTTGAAGCGCCAAGGTGCATCAATACCACTTGTACGACCGGGCAAAAGTACGGCTGCATTTCTTAAGACAGTTCTAAGTCGAATATCAGTGCTTGGTTCAGCCTTCCTGGCAATCCTGGCTGCTGGTCCTTCAGTGATTGAACAAGTGACGCACCTCACTGCATTTCGTGGATTCGCAGGCACATCTGTTCTCATCCTTGTTGGTTGTGCAACTGACACAGCAAGAAAAGTTGAAGCAGAAATAATATCTCAGAAGTACAAGAACATAGAGTTCTATAACATTGATAAGTATGGCCCGTAA

Protein Analysis

558

Amino Acids

60.09

Weight (kDa)

9.61

Isoelectric Point (pI)

36.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SecY PF00344 199 - 531 1.6e-90 SecY
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 219
AccI GTMKAC 1 cut(s) 725
AciI CCGC 3 cut(s) 38, 217, 1242
AclI AACGTT 1 cut(s) 317
AclWI GGATC 6 cut(s) 15, 28, 233, 684, 1346, 1359
AcoI YGGCCR 1 cut(s) 769
AcsI RAATTY 4 cut(s) 253, 526, 657, 1077
AcuI CTGAAG 5 cut(s) 342, 665, 723, 790, 1488
AfaI GTAC 6 cut(s) 811, 944, 1216, 1407, 1423, 1631
AfiI CCNNNNNNNGG 4 cut(s) 431, 497, 963, 1348
AflII CTTAAG 1 cut(s) 1436
AgeI ACCGGT 1 cut(s) 1232
AgsI TTSAA 7 cut(s) 49, 1130, 1249, 1324, 1378, 1514, 1607
AjnI CCWGG 6 cut(s) 284, 759, 1210, 1273, 1479, 1488
AjuI GAANNNNNNNTTGG 2 cut(s) 1171, 1203
AluBI AGCT 8 cut(s) 93, 153, 199, 362, 377, 448, 646, 1205
AluI AGCT 8 cut(s) 93, 153, 199, 362, 377, 448, 646, 1205
Alw21I GWGCWC 1 cut(s) 554
Alw26I GTCTC 3 cut(s) 402, 474, 1027
AlwI GGATC 6 cut(s) 15, 28, 233, 684, 1346, 1359
AoxI GGCC 3 cut(s) 462, 769, 1666
ApeKI GCWGC 5 cut(s) 374, 377, 1256, 1427, 1493
ApoI RAATTY 4 cut(s) 253, 526, 657, 1077
AsiGI ACCGGT 1 cut(s) 1232
AspLEI GCGC 1 cut(s) 1383
AspS9I GGNCC 4 cut(s) 422, 1039, 1499, 1667
AsuC2I CCSGG 2 cut(s) 432, 1413
AsuHPI GGTGA 3 cut(s) 105, 1168, 1222
AsuII TTCGAA 1 cut(s) 338
AvaII GGWCC 3 cut(s) 422, 1039, 1499
BalI TGGCCA 1 cut(s) 771
BamHI GGATCC 2 cut(s) 20, 1351
BarI GAAGNNNNNNTAC 2 cut(s) 768, 800
BbsI GAAGAC 2 cut(s) 592, 631
Bbv12I GWGCWC 1 cut(s) 554
BbvI GCAGC 5 cut(s) 364, 386, 1243, 1414, 1480
BccI CCATC 1 cut(s) 983
BceAI ACGGC 2 cut(s) 48, 1440
BciT130I CCWGG 6 cut(s) 286, 761, 1212, 1275, 1481, 1490
BcnI CCSGG 2 cut(s) 432, 1413
BcoDI GTCTC 3 cut(s) 402, 474, 1027
BfaI CTAG 1 cut(s) 363
BfmI CTRYAG 3 cut(s) 1218, 1257, 1343
BfoI RGCGCY 1 cut(s) 1384
BfrI CTTAAG 1 cut(s) 1436
BglI GCCNNNNNGGC 1 cut(s) 1481
BglII AGATCT 1 cut(s) 1133
BisI GCNGC 7 cut(s) 217, 375, 378, 1242, 1257, 1428, 1494
BlsI GCNGC 7 cut(s) 218, 376, 379, 1243, 1258, 1429, 1495
Bme1390I CCNGG 8 cut(s) 286, 432, 761, 1212, 1275, 1413, 1481, 1490
Bme18I GGWCC 3 cut(s) 422, 1039, 1499
BmgT120I GGNCC 4 cut(s) 422, 1039, 1499, 1667
BmiI GGNNCC 2 cut(s) 22, 1353
BmrFI CCNGG 8 cut(s) 286, 432, 761, 1212, 1275, 1413, 1481, 1490
BmsI GCATC 3 cut(s) 942, 962, 1400
BpiI GAAGAC 2 cut(s) 592, 631
BplI GAGNNNNNCTC 2 cut(s) 314, 346
BpmI CTGGAG 4 cut(s) 105, 196, 591, 1188
Bpu14I TTCGAA 1 cut(s) 338
BpuEI CTTGAG 3 cut(s) 316, 428, 1081
BpuMI CCSGG 2 cut(s) 432, 1413
Bsa29I ATCGAT 1 cut(s) 137
BsaJI CCNNGG 6 cut(s) 243, 285, 555, 1042, 1274, 1384
BsaWI WCCGGW 1 cut(s) 1232
Bsc4I CCNNNNNNNGG 4 cut(s) 431, 497, 963, 1348
Bse118I RCCGGY 1 cut(s) 1232
BseBI CCWGG 6 cut(s) 286, 761, 1212, 1275, 1481, 1490
BseCI ATCGAT 1 cut(s) 137
BseDI CCNNGG 6 cut(s) 243, 285, 555, 1042, 1274, 1384
BseGI GGATG 4 cut(s) 953, 994, 1302, 1566
BseLI CCNNNNNNNGG 4 cut(s) 431, 497, 963, 1348
BseMII CTCAG 5 cut(s) 28, 234, 243, 867, 1637
BseRI GAGGAG 1 cut(s) 588
BseXI GCAGC 5 cut(s) 364, 386, 1243, 1414, 1480
BseYI CCCAGC 1 cut(s) 149
Bsh1285I CGRYCG 1 cut(s) 1412
BshFI GGCC 3 cut(s) 464, 771, 1668
BshTI ACCGGT 1 cut(s) 1232
BshVI ATCGAT 1 cut(s) 137
BsiEI CGRYCG 1 cut(s) 1412
BsiHKAI GWGCWC 1 cut(s) 554
BsiSI CCGG 3 cut(s) 432, 1233, 1412
BslI CCNNNNNNNGG 4 cut(s) 431, 497, 963, 1348
BsmAI GTCTC 3 cut(s) 402, 474, 1027
BsnI GGCC 3 cut(s) 464, 771, 1668
Bsp119I TTCGAA 1 cut(s) 338
Bsp1286I GDGCHC 1 cut(s) 554
Bsp143I GATC 7 cut(s) 20, 134, 138, 238, 676, 1133, 1351
BspACI CCGC 3 cut(s) 38, 217, 1242
BspANI GGCC 3 cut(s) 464, 771, 1668
BspCNI CTCAG 5 cut(s) 27, 233, 242, 866, 1636
BspDI ATCGAT 1 cut(s) 137
BspLI GGNNCC 2 cut(s) 22, 1353
BspMAI CTGCAG 1 cut(s) 1261
BspPI GGATC 6 cut(s) 15, 28, 233, 684, 1346, 1359
BspQI GCTCTTC 1 cut(s) 1212
BspT104I TTCGAA 1 cut(s) 338
BspTI CTTAAG 1 cut(s) 1436
BsrBI CCGCTC 1 cut(s) 219
BsrFI RCCGGY 1 cut(s) 1232
BssAI RCCGGY 1 cut(s) 1232
BssECI CCNNGG 6 cut(s) 243, 285, 555, 1042, 1274, 1384
BssMI GATC 7 cut(s) 20, 134, 138, 238, 676, 1133, 1351
BssNAI GTATAC 1 cut(s) 726
BssT1I CCWWGG 4 cut(s) 243, 555, 1042, 1384
Bst1107I GTATAC 1 cut(s) 726
Bst2UI CCWGG 6 cut(s) 286, 761, 1212, 1275, 1481, 1490
Bst4CI ACNGT 7 cut(s) 392, 509, 536, 970, 1347, 1374, 1444
Bst6I CTCTTC 2 cut(s) 832, 1212
BstAFI CTTAAG 1 cut(s) 1436
BstBI TTCGAA 1 cut(s) 338
BstC8I GCNNGC 2 cut(s) 1225, 1552
BstDEI CTNAG 6 cut(s) 14, 220, 229, 853, 1448, 1623
BstF5I GGATG 4 cut(s) 953, 994, 1302, 1566
BstH2I RGCGCY 1 cut(s) 1384
BstHHI GCGC 1 cut(s) 1383
BstKTI GATC 7 cut(s) 23, 137, 141, 241, 679, 1136, 1354
BstMAI GTCTC 3 cut(s) 402, 474, 1027
BstMBI GATC 7 cut(s) 20, 134, 138, 238, 676, 1133, 1351
BstMCI CGRYCG 1 cut(s) 1412
BstMWI GCNNNNNNNGC 5 cut(s) 150, 461, 1262, 1481, 1490
BstNI CCWGG 6 cut(s) 286, 761, 1212, 1275, 1481, 1490
BstSCI CCNGG 8 cut(s) 284, 430, 759, 1210, 1273, 1411, 1479, 1488
BstSFI CTRYAG 3 cut(s) 1218, 1257, 1343
BstV1I GCAGC 5 cut(s) 364, 386, 1243, 1414, 1480
BstV2I GAAGAC 2 cut(s) 592, 631
BstX2I RGATCY 4 cut(s) 20, 676, 1133, 1351
BstXI CCANNNNNNTGG 1 cut(s) 767
BstYI RGATCY 4 cut(s) 20, 676, 1133, 1351
BstZ17I GTATAC 1 cut(s) 726
Bsu15I ATCGAT 1 cut(s) 137
BsuRI GGCC 3 cut(s) 464, 771, 1668
BsuTUI ATCGAT 1 cut(s) 137
BtsCI GGATG 4 cut(s) 953, 994, 1302, 1566
BtsI GCAGTG 2 cut(s) 1266, 1530
BtsIMutI CAGTG 5 cut(s) 125, 1266, 1467, 1512, 1530
Cac8I GCNNGC 2 cut(s) 1225, 1552
CfoI GCGC 1 cut(s) 1383
Cfr10I RCCGGY 1 cut(s) 1232
Cfr13I GGNCC 4 cut(s) 422, 1039, 1499, 1667
ClaI ATCGAT 1 cut(s) 137
CseI GACGC 1 cut(s) 1531
Csp6I GTAC 6 cut(s) 810, 943, 1215, 1406, 1422, 1630
CspAI ACCGGT 1 cut(s) 1232
CspCI CAANNNNNGTGG 4 cut(s) 1000, 1035, 1099, 1134
CviQI GTAC 6 cut(s) 810, 943, 1215, 1406, 1422, 1630
DdeI CTNAG 6 cut(s) 14, 220, 229, 853, 1448, 1623
DpnI GATC 7 cut(s) 22, 136, 140, 240, 678, 1135, 1353
DpnII GATC 7 cut(s) 20, 134, 138, 238, 676, 1133, 1351
DraI TTTAAA 2 cut(s) 418, 1269
EaeI YGGCCR 1 cut(s) 769
Eam1104I CTCTTC 2 cut(s) 832, 1212
EarI CTCTTC 2 cut(s) 832, 1212
Eco130I CCWWGG 4 cut(s) 243, 555, 1042, 1384
Eco47I GGWCC 3 cut(s) 422, 1039, 1499
Eco57I CTGAAG 5 cut(s) 342, 665, 723, 790, 1488
EcoRII CCWGG 6 cut(s) 284, 759, 1210, 1273, 1479, 1488
EcoT14I CCWWGG 4 cut(s) 243, 555, 1042, 1384
ErhI CCWWGG 4 cut(s) 243, 555, 1042, 1384
FalI AAGNNNNNCTT 2 cut(s) 1123, 1155
FauI CCCGC 1 cut(s) 45
FauNDI CATATG 1 cut(s) 793
FblI GTMKAC 1 cut(s) 725
Fnu4HI GCNGC 7 cut(s) 217, 375, 378, 1242, 1257, 1428, 1494
FokI GGATG 4 cut(s) 940, 1001, 1289, 1553
Fsp4HI GCNGC 7 cut(s) 217, 375, 378, 1242, 1257, 1428, 1494
FspBI CTAG 1 cut(s) 363
GlaI GCGC 1 cut(s) 1382
GluI GCNGC 7 cut(s) 217, 375, 378, 1242, 1257, 1428, 1494
GsaI CCCAGC 1 cut(s) 153
GsuI CTGGAG 4 cut(s) 105, 196, 591, 1188
HaeII RGCGCY 1 cut(s) 1384
HaeIII GGCC 3 cut(s) 464, 771, 1668
HapII CCGG 3 cut(s) 432, 1233, 1412
HgaI GACGC 1 cut(s) 1531
HhaI GCGC 1 cut(s) 1383
Hin6I GCGC 1 cut(s) 1381
HinP1I GCGC 1 cut(s) 1381
HincII GTYRAC 1 cut(s) 505
HindII GTYRAC 1 cut(s) 505
HindIII AAGCTT 1 cut(s) 197
HinfI GANTC 3 cut(s) 560, 882, 1545
HpaI GTTAAC 1 cut(s) 505
HpaII CCGG 3 cut(s) 432, 1233, 1412
HphI GGTGA 3 cut(s) 105, 1168, 1222
Hpy166II GTNNAC 4 cut(s) 505, 602, 726, 1370
Hpy188I TCNGA 7 cut(s) 17, 165, 223, 259, 684, 902, 1626
Hpy188III TCNNGA 6 cut(s) 471, 570, 986, 1061, 1167, 1355
Hpy8I GTNNAC 4 cut(s) 505, 602, 726, 1370
Hpy99I CGWCG 1 cut(s) 962
HpyAV CCTTC 7 cut(s) 337, 686, 794, 1036, 1327, 1486, 1512
HpyCH4III ACNGT 7 cut(s) 392, 509, 536, 970, 1347, 1374, 1444
HpyCH4IV ACGT 1 cut(s) 317
HpyF10VI GCNNNNNNNGC 5 cut(s) 150, 461, 1262, 1481, 1490
HpyF3I CTNAG 6 cut(s) 14, 220, 229, 853, 1448, 1623
HpySE526I ACGT 1 cut(s) 317
HspAI GCGC 1 cut(s) 1381
KspAI GTTAAC 1 cut(s) 505
Kzo9I GATC 7 cut(s) 20, 134, 138, 238, 676, 1133, 1351
LguI GCTCTTC 1 cut(s) 1212
Lsp1109I GCAGC 5 cut(s) 364, 386, 1243, 1414, 1480
LweI GCATC 3 cut(s) 942, 962, 1400
MaeI CTAG 1 cut(s) 363
MaeII ACGT 1 cut(s) 317
MaeIII GTNAC 3 cut(s) 832, 992, 1519
MalI GATC 7 cut(s) 22, 136, 140, 240, 678, 1135, 1353
MbiI CCGCTC 1 cut(s) 219
MboI GATC 7 cut(s) 20, 134, 138, 238, 676, 1133, 1351
MfeI CAATTG 1 cut(s) 765
MflI RGATCY 4 cut(s) 20, 676, 1133, 1351
MhlI GDGCHC 1 cut(s) 554
MlsI TGGCCA 1 cut(s) 771
MluCI AATT 7 cut(s) 253, 265, 526, 657, 714, 765, 1077
MluNI TGGCCA 1 cut(s) 771
MmeI TCCRAC 3 cut(s) 502, 982, 1329
Mox20I TGGCCA 1 cut(s) 771
MscI TGGCCA 1 cut(s) 771
MseI TTAA 7 cut(s) 417, 504, 1086, 1152, 1268, 1310, 1437
Msp20I TGGCCA 1 cut(s) 771
MspA1I CMGCKG 1 cut(s) 377
MspCI CTTAAG 1 cut(s) 1436
MspI CCGG 3 cut(s) 432, 1233, 1412
MspR9I CCNGG 8 cut(s) 286, 432, 761, 1212, 1275, 1413, 1481, 1490
MunI CAATTG 1 cut(s) 765
MvaI CCWGG 6 cut(s) 286, 761, 1212, 1275, 1481, 1490
MwoI GCNNNNNNNGC 5 cut(s) 150, 461, 1262, 1481, 1490
NciI CCSGG 2 cut(s) 432, 1413
NdeI CATATG 1 cut(s) 793
NdeII GATC 7 cut(s) 20, 134, 138, 238, 676, 1133, 1351
NlaIV GGNNCC 2 cut(s) 22, 1353
NmuCI GTSAC 1 cut(s) 1519
NspV TTCGAA 1 cut(s) 338
PciSI GCTCTTC 1 cut(s) 1212
PfeI GAWTC 3 cut(s) 560, 882, 1545
PinAI ACCGGT 1 cut(s) 1232
PkrI GCNGC 7 cut(s) 218, 376, 379, 1243, 1258, 1429, 1495
Psp1406I AACGTT 1 cut(s) 317
Psp6I CCWGG 6 cut(s) 284, 759, 1210, 1273, 1479, 1488
PspFI CCCAGC 1 cut(s) 149
PspGI CCWGG 6 cut(s) 284, 759, 1210, 1273, 1479, 1488
PspN4I GGNNCC 2 cut(s) 22, 1353
PspPI GGNCC 4 cut(s) 422, 1039, 1499, 1667
PstI CTGCAG 1 cut(s) 1261
PsuI RGATCY 4 cut(s) 20, 676, 1133, 1351
PvuII CAGCTG 1 cut(s) 377
RsaI GTAC 6 cut(s) 811, 944, 1216, 1407, 1423, 1631
RsaNI GTAC 6 cut(s) 810, 943, 1215, 1406, 1422, 1630
SapI GCTCTTC 1 cut(s) 1212
SaqAI TTAA 7 cut(s) 417, 504, 1086, 1152, 1268, 1310, 1437
SatI GCNGC 7 cut(s) 217, 375, 378, 1242, 1257, 1428, 1494
Sau3AI GATC 7 cut(s) 20, 134, 138, 238, 676, 1133, 1351
Sau96I GGNCC 4 cut(s) 422, 1039, 1499, 1667
ScrFI CCNGG 8 cut(s) 286, 432, 761, 1212, 1275, 1413, 1481, 1490
SduI GDGCHC 1 cut(s) 554
SfaNI GCATC 3 cut(s) 942, 962, 1400
SfcI CTRYAG 3 cut(s) 1218, 1257, 1343
SfuI TTCGAA 1 cut(s) 338
SinI GGWCC 3 cut(s) 422, 1039, 1499
SmlI CTYRAG 4 cut(s) 331, 443, 1096, 1436
SmoI CTYRAG 4 cut(s) 331, 443, 1096, 1436
Sse9I AATT 7 cut(s) 253, 265, 526, 657, 714, 765, 1077
SsiI CCGC 3 cut(s) 38, 217, 1242
SspI AATATT 1 cut(s) 1185
SspMI CTAG 1 cut(s) 363
StyD4I CCNGG 8 cut(s) 284, 430, 759, 1210, 1273, 1411, 1479, 1488
StyI CCWWGG 4 cut(s) 243, 555, 1042, 1384
TaaI ACNGT 7 cut(s) 392, 509, 536, 970, 1347, 1374, 1444
TaiI ACGT 1 cut(s) 320
TaqI TCGA 5 cut(s) 137, 338, 472, 632, 1453
TasI AATT 7 cut(s) 253, 265, 526, 657, 714, 765, 1077
TatI WGTACW 2 cut(s) 809, 1629
TauI GCSGC 2 cut(s) 219, 1244
TfiI GAWTC 3 cut(s) 560, 882, 1545
Tru1I TTAA 7 cut(s) 417, 504, 1086, 1152, 1268, 1310, 1437
Tru9I TTAA 7 cut(s) 417, 504, 1086, 1152, 1268, 1310, 1437
TscAI CASTG 5 cut(s) 132, 1266, 1467, 1512, 1537
TseFI GTSAC 1 cut(s) 1519
TseI GCWGC 5 cut(s) 374, 377, 1256, 1427, 1493
Tsp45I GTSAC 1 cut(s) 1519
TspDTI ATGAA 1 cut(s) 610
TspGWI ACGGA 2 cut(s) 148, 946
TspRI CASTG 5 cut(s) 132, 1266, 1467, 1512, 1537
Vha464I CTTAAG 1 cut(s) 1436
VpaK11BI GGWCC 3 cut(s) 422, 1039, 1499
XapI RAATTY 4 cut(s) 253, 526, 657, 1077
XmiI GTMKAC 1 cut(s) 725
XspI CTAG 1 cut(s) 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.