Rh4AG123600

Preprotein translocase subunit SCY1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0010079
Physical Location & Seq
Reverse (-)
26682889 .. 26688701
5813 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG123600.1

Sequence Viewer

Length: 1449 bp
ATGTTGATAACGGTCAGAGGACCCTTTTGTTCTTCTTCTCCGCTCTGCTTCAACAGCTTCTCTTATCCAACGCTGAAACGTTCAGTATGCAAACCCAGATTCTCTGTTCCCAGAAAACCCAACAGCATTACTTGTTGGAAGCTTGGCGTTCTATCCAACAGCTCTGAAGCCTCAGTTTTTGATCCCTTGGGTATAAATTCAGATATACATTCTGGTCTAAATGCTACCTGGGATAGTTTCCTATGCCTATTTTCAAAACCGTTAGAGAGTGCTTCAAGTTCTAAGAAGGAGAAATCTAACTCCACTCGAGGATTAGCAGCTGCCATCGAAGACAGTTCCATTGATTTTGGAGACTTTTTCAAAGGCCCATTACCAGGGAAGTTTCTCATGCTGTTGGGATATCTGGCCTTATCTCGACTTGGAATATATATACCTCTTGGTGGGGTAAACCGTGAGGCTTTTGTTGGAAATTTGGACCAGAACAGTTTGTTGAGCACTTTGGATTCATTTTCTGGAGGTGGCATTGGCCGACTTGGTATATGTTCACTTGGTATTGTTCCCTTCATCAATGCCCAGATTGTCTTCCAGCTCCTTGCACAAGTATATCCCAAGTTGCAGGATCTTCAGAAAAAAGAAGGTGAAGCAGGAAGAAAGAAAGTTCTGCGGTATACACAATATGCTTCGGTTGGGTTTGCAGTAGTCCAGGCAATTGGCCAAGTATTCTTCCTTCGCCCATATGTCAATGACTTCAGTACGGAGTGGGTTCTCTCATCTGTGATCTTATTGACTCTTGGCTCAGTATTGACAACATACATTGGAGAACGAATCTCAGACCTAAAACTTGGGAATGGCACATCTCTTTTGATATTCACAAGCATCATCTCCTACTTTCCAGCATCTGTTGGTAGGACTGTTGCACAGGCATTCCAAGATGGTAACTATGTTGGACTCGCCACCATCGTTGCCTCCTTTTTTGTTTTGGTCCTTGGAATTGTGTATGTTCAGGAAGCAGAAAGGAAAATTCCAATTAATTATGCCTCAAGGTACACCAGCAGAAGTGCAGGTCCTCAAAGGTCTGCTTACCTCCCCTTTAAGGTAAATAGTTCTGGAGTAATGCCAATAATATTTTCTACATCATCATTAGCTCTTCCTGGCACTTTAGCACGCTTCACTGGTTTAGCTCCATTGAAAACGGCTGCAGTGGCTTTAAATCCAGGGGGTTCATTCTATCTCCCCACCAACATCCTTTTAATAGCCTTCTTCAACTACTACTACACTTTCCTACAATTGGATCCTGATGATGTAAGTGAACAGTTGAAGCGCCAAGGGGCATCAATTCCACTTGTACGGCCGGGTAAAAGCACAGCTGCATTTCTTAAGACGGTAATTTTCTTCTTTGTTAAGGAAAATTTGTGGTCAATTATTTGCATTATTGAGTGGAGTGTTTAA

Protein Analysis

482

Amino Acids

52.51

Weight (kDa)

9.38

Isoelectric Point (pI)

31.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SecY PF00344 180 - 463 9.9e-77 SecY
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1052
AccBSI CCGCTC 1 cut(s) 43
AccI GTMKAC 1 cut(s) 668
AciI CCGC 2 cut(s) 41, 664
AclI AACGTT 1 cut(s) 79
AclWI GGATC 4 cut(s) 176, 627, 1286, 1299
AcoI YGGCCR 3 cut(s) 526, 712, 1349
AcsI RAATTY 4 cut(s) 196, 469, 1020, 1408
AcuI CTGAAG 3 cut(s) 186, 608, 733
AfaI GTAC 3 cut(s) 754, 1046, 1347
AfiI CCNNNNNNNGG 4 cut(s) 374, 440, 1093, 1288
AflII CTTAAG 1 cut(s) 1376
AgsI TTSAA 7 cut(s) 52, 255, 276, 361, 1189, 1264, 1318
AjnI CCWGG 5 cut(s) 227, 373, 702, 1150, 1213
AjuI GAANNNNNNNTTGG 2 cut(s) 1111, 1143
AluBI AGCT 8 cut(s) 57, 142, 162, 320, 589, 1145, 1181, 1367
AluI AGCT 8 cut(s) 57, 142, 162, 320, 589, 1145, 1181, 1367
Alw21I GWGCWC 1 cut(s) 497
Alw26I GTCTC 1 cut(s) 345
AlwI GGATC 4 cut(s) 176, 627, 1286, 1299
AlwNI CAGNNNCTG 1 cut(s) 899
Ama87I CYCGRG 1 cut(s) 306
AoxI GGCC 5 cut(s) 364, 405, 526, 712, 1349
ApeKI GCWGC 4 cut(s) 317, 320, 1196, 1367
ApoI RAATTY 4 cut(s) 196, 469, 1020, 1408
AseI ATTAAT 1 cut(s) 1029
AspLEI GCGC 1 cut(s) 1323
AspS9I GGNCC 5 cut(s) 20, 365, 475, 982, 1064
AsuC2I CCSGG 1 cut(s) 1353
AsuHPI GGTGA 1 cut(s) 650
AvaI CYCGRG 1 cut(s) 306
AvaII GGWCC 4 cut(s) 20, 475, 982, 1064
BalI TGGCCA 1 cut(s) 714
BamHI GGATCC 1 cut(s) 1291
BarI GAAGNNNNNNTAC 2 cut(s) 711, 743
BbsI GAAGAC 2 cut(s) 336, 574
Bbv12I GWGCWC 1 cut(s) 497
BbvI GCAGC 4 cut(s) 307, 329, 1183, 1354
BccI CCATC 3 cut(s) 332, 926, 965
BceAI ACGGC 2 cut(s) 1209, 1364
BciT130I CCWGG 5 cut(s) 229, 375, 704, 1152, 1215
BcnI CCSGG 1 cut(s) 1353
BcoDI GTCTC 1 cut(s) 345
BfmI CTRYAG 1 cut(s) 1197
BfoI RGCGCY 1 cut(s) 1324
BfrI CTTAAG 1 cut(s) 1376
BfuAI ACCTGC 1 cut(s) 1052
BisI GCNGC 4 cut(s) 318, 321, 1197, 1368
BlsI GCNGC 4 cut(s) 319, 322, 1198, 1369
Bme1390I CCNGG 6 cut(s) 229, 375, 704, 1152, 1215, 1353
Bme18I GGWCC 4 cut(s) 20, 475, 982, 1064
BmeT110I CYCGRG 1 cut(s) 306
BmgT120I GGNCC 5 cut(s) 20, 365, 475, 982, 1064
BmiI GGNNCC 2 cut(s) 22, 1293
BmrFI CCNGG 6 cut(s) 229, 375, 704, 1152, 1215, 1353
BmsI GCATC 3 cut(s) 885, 905, 1340
BpiI GAAGAC 2 cut(s) 336, 574
BpmI CTGGAG 2 cut(s) 534, 1128
BpuEI CTTGAG 1 cut(s) 1024
BpuMI CCSGG 1 cut(s) 1353
BsaJI CCNNGG 6 cut(s) 186, 228, 374, 985, 1214, 1324
Bsc4I CCNNNNNNNGG 4 cut(s) 374, 440, 1093, 1288
Bse1I ACTGG 1 cut(s) 1177
BseBI CCWGG 5 cut(s) 229, 375, 704, 1152, 1215
BseDI CCNNGG 6 cut(s) 186, 228, 374, 985, 1214, 1324
BseGI GGATG 1 cut(s) 1242
BseLI CCNNNNNNNGG 4 cut(s) 374, 440, 1093, 1288
BseMII CTCAG 3 cut(s) 186, 810, 843
BseNI ACTGG 1 cut(s) 1177
BseX3I CGGCCG 1 cut(s) 1349
BseXI GCAGC 4 cut(s) 307, 329, 1183, 1354
BsgI GTGCAG 1 cut(s) 1080
Bsh1285I CGRYCG 1 cut(s) 1352
BshFI GGCC 5 cut(s) 366, 407, 528, 714, 1351
BsiEI CGRYCG 1 cut(s) 1352
BsiHKAI GWGCWC 1 cut(s) 497
BsiHKCI CYCGRG 1 cut(s) 306
BsiSI CCGG 1 cut(s) 1352
BslI CCNNNNNNNGG 4 cut(s) 374, 440, 1093, 1288
BsmAI GTCTC 1 cut(s) 345
BsmI GAATGC 1 cut(s) 923
BsnI GGCC 5 cut(s) 366, 407, 528, 714, 1351
BsoBI CYCGRG 1 cut(s) 306
Bsp1286I GDGCHC 1 cut(s) 497
Bsp143I GATC 4 cut(s) 181, 619, 777, 1291
BspACI CCGC 2 cut(s) 41, 664
BspANI GGCC 5 cut(s) 366, 407, 528, 714, 1351
BspCNI CTCAG 3 cut(s) 185, 809, 842
BspLI GGNNCC 2 cut(s) 22, 1293
BspMAI CTGCAG 1 cut(s) 1201
BspMI ACCTGC 1 cut(s) 1052
BspPI GGATC 4 cut(s) 176, 627, 1286, 1299
BspQI GCTCTTC 1 cut(s) 1152
BspTI CTTAAG 1 cut(s) 1376
BsrBI CCGCTC 1 cut(s) 43
BsrI ACTGG 1 cut(s) 1177
BssECI CCNNGG 6 cut(s) 186, 228, 374, 985, 1214, 1324
BssMI GATC 4 cut(s) 181, 619, 777, 1291
BssNAI GTATAC 1 cut(s) 669
BssT1I CCWWGG 3 cut(s) 186, 985, 1324
Bst1107I GTATAC 1 cut(s) 669
Bst2UI CCWGG 5 cut(s) 229, 375, 704, 1152, 1215
Bst4CI ACNGT 8 cut(s) 13, 261, 335, 452, 485, 913, 1314, 1384
Bst6I CTCTTC 1 cut(s) 1152
BstAFI CTTAAG 1 cut(s) 1376
BstC8I GCNNGC 1 cut(s) 1165
BstDEI CTNAG 4 cut(s) 172, 282, 796, 829
BstF5I GGATG 1 cut(s) 1242
BstH2I RGCGCY 1 cut(s) 1324
BstHHI GCGC 1 cut(s) 1323
BstKTI GATC 4 cut(s) 184, 622, 780, 1294
BstMAI GTCTC 1 cut(s) 345
BstMBI GATC 4 cut(s) 181, 619, 777, 1291
BstMCI CGRYCG 1 cut(s) 1352
BstMWI GCNNNNNNNGC 2 cut(s) 54, 1202
BstNI CCWGG 5 cut(s) 229, 375, 704, 1152, 1215
BstSCI CCNGG 6 cut(s) 227, 373, 702, 1150, 1213, 1351
BstSFI CTRYAG 1 cut(s) 1197
BstV1I GCAGC 4 cut(s) 307, 329, 1183, 1354
BstV2I GAAGAC 2 cut(s) 336, 574
BstX2I RGATCY 2 cut(s) 619, 1291
BstXI CCANNNNNNTGG 1 cut(s) 710
BstYI RGATCY 2 cut(s) 619, 1291
BstZ17I GTATAC 1 cut(s) 669
BstZI CGGCCG 1 cut(s) 1349
BsuRI GGCC 5 cut(s) 366, 407, 528, 714, 1351
BtsCI GGATG 1 cut(s) 1242
BtsI GCAGTG 1 cut(s) 1206
BtsIMutI CAGTG 2 cut(s) 1170, 1206
BveI ACCTGC 1 cut(s) 1052
Cac8I GCNNGC 1 cut(s) 1165
CaiI CAGNNNCTG 1 cut(s) 899
CfoI GCGC 1 cut(s) 1323
Cfr13I GGNCC 5 cut(s) 20, 365, 475, 982, 1064
Csp6I GTAC 3 cut(s) 753, 1045, 1346
CspCI CAANNNNNGTGG 2 cut(s) 943, 978
CviAII CATG 1 cut(s) 388
CviQI GTAC 3 cut(s) 753, 1045, 1346
DdeI CTNAG 4 cut(s) 172, 282, 796, 829
DpnI GATC 4 cut(s) 183, 621, 779, 1293
DpnII GATC 4 cut(s) 181, 619, 777, 1291
DraI TTTAAA 1 cut(s) 1209
EaeI YGGCCR 3 cut(s) 526, 712, 1349
EagI CGGCCG 1 cut(s) 1349
Eam1104I CTCTTC 1 cut(s) 1152
EarI CTCTTC 1 cut(s) 1152
EclXI CGGCCG 1 cut(s) 1349
Eco130I CCWWGG 3 cut(s) 186, 985, 1324
Eco32I GATATC 1 cut(s) 401
Eco47I GGWCC 4 cut(s) 20, 475, 982, 1064
Eco52I CGGCCG 1 cut(s) 1349
Eco57I CTGAAG 3 cut(s) 186, 608, 733
Eco88I CYCGRG 1 cut(s) 306
EcoO109I RGGNCCY 2 cut(s) 20, 1064
EcoRII CCWGG 5 cut(s) 227, 373, 702, 1150, 1213
EcoRV GATATC 1 cut(s) 401
EcoT14I CCWWGG 3 cut(s) 186, 985, 1324
ErhI CCWWGG 3 cut(s) 186, 985, 1324
FaeI CATG 1 cut(s) 391
FalI AAGNNNNNCTT 2 cut(s) 1063, 1095
FatI CATG 1 cut(s) 387
FauNDI CATATG 1 cut(s) 736
FblI GTMKAC 1 cut(s) 668
Fnu4HI GCNGC 4 cut(s) 318, 321, 1197, 1368
FokI GGATG 1 cut(s) 1229
Fsp4HI GCNGC 4 cut(s) 318, 321, 1197, 1368
GlaI GCGC 1 cut(s) 1322
GluI GCNGC 4 cut(s) 318, 321, 1197, 1368
GsuI CTGGAG 2 cut(s) 534, 1128
HaeII RGCGCY 1 cut(s) 1324
HaeIII GGCC 5 cut(s) 366, 407, 528, 714, 1351
HapII CCGG 1 cut(s) 1352
HhaI GCGC 1 cut(s) 1323
Hin1II CATG 1 cut(s) 391
Hin6I GCGC 1 cut(s) 1321
HinP1I GCGC 1 cut(s) 1321
HindIII AAGCTT 1 cut(s) 140
HinfI GANTC 5 cut(s) 99, 503, 787, 825, 948
HpaII CCGG 1 cut(s) 1352
HphI GGTGA 1 cut(s) 650
Hpy166II GTNNAC 5 cut(s) 448, 545, 669, 1047, 1310
Hpy188I TCNGA 5 cut(s) 17, 166, 202, 627, 832
Hpy188III TCNNGA 5 cut(s) 414, 513, 1004, 1107, 1295
Hpy8I GTNNAC 5 cut(s) 448, 545, 669, 1047, 1310
HpyAV CCTTC 5 cut(s) 280, 571, 629, 737, 1267
HpyCH4III ACNGT 8 cut(s) 13, 261, 335, 452, 485, 913, 1314, 1384
HpyCH4IV ACGT 1 cut(s) 79
HpyCH4V TGCA 9 cut(s) 90, 596, 616, 695, 917, 1061, 1199, 1370, 1428
HpyF10VI GCNNNNNNNGC 2 cut(s) 54, 1202
HpyF3I CTNAG 4 cut(s) 172, 282, 796, 829
HpySE526I ACGT 1 cut(s) 79
Hsp92II CATG 1 cut(s) 391
HspAI GCGC 1 cut(s) 1321
Kzo9I GATC 4 cut(s) 181, 619, 777, 1291
LguI GCTCTTC 1 cut(s) 1152
LmnI GCTCC 2 cut(s) 594, 1186
Lsp1109I GCAGC 4 cut(s) 307, 329, 1183, 1354
LweI GCATC 3 cut(s) 885, 905, 1340
MaeII ACGT 1 cut(s) 79
MaeIII GTNAC 1 cut(s) 935
MalI GATC 4 cut(s) 183, 621, 779, 1293
MbiI CCGCTC 1 cut(s) 43
MboI GATC 4 cut(s) 181, 619, 777, 1291
MfeI CAATTG 2 cut(s) 708, 1286
MflI RGATCY 2 cut(s) 619, 1291
MhlI GDGCHC 1 cut(s) 497
MlsI TGGCCA 1 cut(s) 714
MluNI TGGCCA 1 cut(s) 714
MlyI GAGTC 2 cut(s) 781, 942
MmeI TCCRAC 5 cut(s) 92, 116, 180, 445, 925
Mox20I TGGCCA 1 cut(s) 714
MscI TGGCCA 1 cut(s) 714
MseI TTAA 7 cut(s) 1029, 1092, 1208, 1250, 1377, 1401, 1447
Msp20I TGGCCA 1 cut(s) 714
MspA1I CMGCKG 2 cut(s) 320, 1367
MspCI CTTAAG 1 cut(s) 1376
MspI CCGG 1 cut(s) 1352
MspR9I CCNGG 6 cut(s) 229, 375, 704, 1152, 1215, 1353
MunI CAATTG 2 cut(s) 708, 1286
Mva1269I GAATGC 1 cut(s) 923
MvaI CCWGG 5 cut(s) 229, 375, 704, 1152, 1215
MwoI GCNNNNNNNGC 2 cut(s) 54, 1202
NciI CCSGG 1 cut(s) 1353
NdeI CATATG 1 cut(s) 736
NdeII GATC 4 cut(s) 181, 619, 777, 1291
NlaIII CATG 1 cut(s) 391
NlaIV GGNNCC 2 cut(s) 22, 1293
PaeR7I CTCGAG 1 cut(s) 306
PciSI GCTCTTC 1 cut(s) 1152
PcsI WCGNNNNNNNCGW 1 cut(s) 957
PctI GAATGC 1 cut(s) 923
PfeI GAWTC 3 cut(s) 99, 503, 825
PkrI GCNGC 4 cut(s) 319, 322, 1198, 1369
PleI GAGTC 2 cut(s) 781, 942
PpsI GAGTC 2 cut(s) 781, 942
PpuMI RGGWCCY 2 cut(s) 20, 1064
PshBI ATTAAT 1 cut(s) 1029
Psp1406I AACGTT 1 cut(s) 79
Psp5II RGGWCCY 2 cut(s) 20, 1064
Psp6I CCWGG 5 cut(s) 227, 373, 702, 1150, 1213
PspGI CCWGG 5 cut(s) 227, 373, 702, 1150, 1213
PspN4I GGNNCC 2 cut(s) 22, 1293
PspPI GGNCC 5 cut(s) 20, 365, 475, 982, 1064
PspPPI RGGWCCY 2 cut(s) 20, 1064
PspXI VCTCGAGB 1 cut(s) 306
PstI CTGCAG 1 cut(s) 1201
PstNI CAGNNNCTG 1 cut(s) 899
PsuI RGATCY 2 cut(s) 619, 1291
PvuII CAGCTG 2 cut(s) 320, 1367
RsaI GTAC 3 cut(s) 754, 1046, 1347
RsaNI GTAC 3 cut(s) 753, 1045, 1346
SapI GCTCTTC 1 cut(s) 1152
SaqAI TTAA 7 cut(s) 1029, 1092, 1208, 1250, 1377, 1401, 1447
SatI GCNGC 4 cut(s) 318, 321, 1197, 1368
Sau3AI GATC 4 cut(s) 181, 619, 777, 1291
Sau96I GGNCC 5 cut(s) 20, 365, 475, 982, 1064
SchI GAGTC 2 cut(s) 781, 942
ScrFI CCNGG 6 cut(s) 229, 375, 704, 1152, 1215, 1353
SduI GDGCHC 1 cut(s) 497
SfaNI GCATC 3 cut(s) 885, 905, 1340
SfcI CTRYAG 1 cut(s) 1197
Sfr274I CTCGAG 1 cut(s) 306
SinI GGWCC 4 cut(s) 20, 475, 982, 1064
SlaI CTCGAG 1 cut(s) 306
SmlI CTYRAG 3 cut(s) 306, 1039, 1376
SmoI CTYRAG 3 cut(s) 306, 1039, 1376
SsiI CCGC 2 cut(s) 41, 664
SspI AATATT 1 cut(s) 1125
StyD4I CCNGG 6 cut(s) 227, 373, 702, 1150, 1213, 1351
StyI CCWWGG 3 cut(s) 186, 985, 1324
TaaI ACNGT 8 cut(s) 13, 261, 335, 452, 485, 913, 1314, 1384
TaiI ACGT 1 cut(s) 82
TaqI TCGA 3 cut(s) 307, 327, 415
TfiI GAWTC 3 cut(s) 99, 503, 825
Tru1I TTAA 7 cut(s) 1029, 1092, 1208, 1250, 1377, 1401, 1447
Tru9I TTAA 7 cut(s) 1029, 1092, 1208, 1250, 1377, 1401, 1447
TscAI CASTG 2 cut(s) 1177, 1206
TseI GCWGC 4 cut(s) 317, 320, 1196, 1367
TspDTI ATGAA 3 cut(s) 495, 553, 1212
TspGWI ACGGA 1 cut(s) 770
TspRI CASTG 2 cut(s) 1177, 1206
Vha464I CTTAAG 1 cut(s) 1376
VpaK11BI GGWCC 4 cut(s) 20, 475, 982, 1064
VspI ATTAAT 1 cut(s) 1029
XapI RAATTY 4 cut(s) 196, 469, 1020, 1408
XhoI CTCGAG 1 cut(s) 306
XmiI GTMKAC 1 cut(s) 668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.