Rh4DG116100

Preprotein translocase subunit SCY1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0010079
Physical Location & Seq
Reverse (-)
18363671 .. 18369658
5988 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG116100.1

Sequence Viewer

Length: 1173 bp
ATGTTGATAACGGTCAGAGGACCCTTTTGTTCTTCTTCTCCGCTCTGCTTCAACAGCTTCTCTTATCCAACGCTGAAACGTTCAGTATTCAAAACCAGATTCTCTGTTCCCAGAAAACCCAACAGCATTACTTGTTGGAAGCTTGGCGTTCTATCCAACAGCTCTGAAGCCTCAGTTTTTGATCCCTTGGGTATAAATTCAGATGTACATTCTGGTCTAAATGCTACCTTGGATAATTTCCTATGGCTATTTTCAAAACCGTTAGAGAGTGCTTCAAGTTCTAAGAAGGAGAAATCGAACTCCACTCGAGGATTAGCAGCTGCCATAGAAGACAGTTCCATTGATTTTGGAGACTTTTTCAAAGGCCCATTACCAGGGAAGTTTCTCATGCTGTTGGGATATCTGGCCTTATCTCGACTTGGAATATATATACCTCTTGGTGGGGTAAACCGTGAGGCTTTTGTTGGAAATTTGGACCAGAACAGTTTGTTGAGCACTTTGGATTCATTTTCTGGAGGCGGCATTGGCCGACTCGGTATATGTTCACTTGGTATTGTTCCCTTCATCAATGCCCAGATTGTCTTCCAGCTCCTTGCACAAGTATATCCCAAGTTGCAGGATCTTCAGAAAAAAGAAGGTGAAGCAGGAAGAAAGAAAGTTCTGCGGTATACACAATATGCTTCGGTTGGGTTTGCAGTAGTCCAGGCAATTGGCCAAGTATTCTTCCTTCGCCCATATGTCAATGACTTCAGTACGGAGTGGGTTCTCTCATCTGTGATCTTATTGACTCTTGGCTCAGTATTGACAACATACATTGGAGAACGAATCTCAGACCTAAAACTTGGGAATGGCACATCTCTTTTGATATTCACAAGCATCATCTCCTACTTTCCAGCATCTGTTGGTAGGACTGTTGCACAGGCATTCCAAGATGGTAACTATGTTGGACTCGCCACCATCGTTGCCTCCTTTTTTGTGTTGGTCCTTGGAATTGTGTATGTTCAGGAAGCAGAAAGGAAAATTCCAATTAATTATGCCTCAAGGTACACCAGCAGAAGTGCAGGTCCTCAAAGGTCTGCTTACCTCCCCTTTAAGGTCTGTACTTCAGTTTCTTCCTCACTTTCCAATGTTGAGAGTTGCAGATGCTTTTGTGTTCATCAAGCTAATTATTAG

Protein Analysis

390

Amino Acids

42.48

Weight (kDa)

9.31

Isoelectric Point (pI)

29.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SecY PF00344 180 - 369 1.4e-48 SecY
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1052
AccBSI CCGCTC 1 cut(s) 43
AccI GTMKAC 1 cut(s) 668
AciI CCGC 3 cut(s) 41, 519, 664
AclI AACGTT 1 cut(s) 79
AclWI GGATC 2 cut(s) 176, 627
AcoI YGGCCR 2 cut(s) 526, 712
AcsI RAATTY 3 cut(s) 196, 469, 1020
AcuI CTGAAG 4 cut(s) 186, 608, 733, 1089
AfaI GTAC 4 cut(s) 207, 754, 1046, 1102
AfiI CCNNNNNNNGG 3 cut(s) 374, 440, 1093
AgsI TTSAA 5 cut(s) 52, 91, 255, 276, 361
AjnI CCWGG 2 cut(s) 373, 702
AluBI AGCT 6 cut(s) 57, 142, 162, 320, 589, 1163
AluI AGCT 6 cut(s) 57, 142, 162, 320, 589, 1163
Alw21I GWGCWC 1 cut(s) 497
Alw26I GTCTC 1 cut(s) 345
AlwI GGATC 2 cut(s) 176, 627
AlwNI CAGNNNCTG 1 cut(s) 899
Ama87I CYCGRG 1 cut(s) 306
AoxI GGCC 4 cut(s) 364, 405, 526, 712
ApeKI GCWGC 2 cut(s) 317, 320
ApoI RAATTY 3 cut(s) 196, 469, 1020
AseI ATTAAT 1 cut(s) 1029
AspS9I GGNCC 5 cut(s) 20, 365, 475, 982, 1064
AsuHPI GGTGA 1 cut(s) 650
AvaI CYCGRG 1 cut(s) 306
AvaII GGWCC 4 cut(s) 20, 475, 982, 1064
BalI TGGCCA 1 cut(s) 714
BarI GAAGNNNNNNTAC 2 cut(s) 711, 743
BbsI GAAGAC 2 cut(s) 336, 574
Bbv12I GWGCWC 1 cut(s) 497
BbvI GCAGC 2 cut(s) 307, 329
BccI CCATC 2 cut(s) 926, 965
BciT130I CCWGG 2 cut(s) 375, 704
BcoDI GTCTC 1 cut(s) 345
BfuAI ACCTGC 1 cut(s) 1052
BisI GCNGC 3 cut(s) 318, 321, 520
BlsI GCNGC 3 cut(s) 319, 322, 521
Bme1390I CCNGG 2 cut(s) 375, 704
Bme18I GGWCC 4 cut(s) 20, 475, 982, 1064
BmeT110I CYCGRG 1 cut(s) 306
BmgT120I GGNCC 5 cut(s) 20, 365, 475, 982, 1064
BmiI GGNNCC 1 cut(s) 22
BmrFI CCNGG 2 cut(s) 375, 704
BmsI GCATC 3 cut(s) 885, 905, 1133
BpiI GAAGAC 2 cut(s) 336, 574
BpmI CTGGAG 1 cut(s) 534
BpuEI CTTGAG 1 cut(s) 1024
BsaJI CCNNGG 4 cut(s) 186, 228, 374, 985
Bsc4I CCNNNNNNNGG 3 cut(s) 374, 440, 1093
BseBI CCWGG 2 cut(s) 375, 704
BseDI CCNNGG 4 cut(s) 186, 228, 374, 985
BseLI CCNNNNNNNGG 3 cut(s) 374, 440, 1093
BseMII CTCAG 3 cut(s) 186, 810, 843
BseXI GCAGC 2 cut(s) 307, 329
BsgI GTGCAG 1 cut(s) 1080
BshFI GGCC 4 cut(s) 366, 407, 528, 714
BsiHKAI GWGCWC 1 cut(s) 497
BsiHKCI CYCGRG 1 cut(s) 306
BslI CCNNNNNNNGG 3 cut(s) 374, 440, 1093
BsmAI GTCTC 1 cut(s) 345
BsmI GAATGC 1 cut(s) 923
BsnI GGCC 4 cut(s) 366, 407, 528, 714
BsoBI CYCGRG 1 cut(s) 306
Bsp1286I GDGCHC 1 cut(s) 497
Bsp1407I TGTACA 1 cut(s) 205
Bsp143I GATC 3 cut(s) 181, 619, 777
BspACI CCGC 3 cut(s) 41, 519, 664
BspANI GGCC 4 cut(s) 366, 407, 528, 714
BspCNI CTCAG 3 cut(s) 185, 809, 842
BspLI GGNNCC 1 cut(s) 22
BspMI ACCTGC 1 cut(s) 1052
BspPI GGATC 2 cut(s) 176, 627
BsrBI CCGCTC 1 cut(s) 43
BsrGI TGTACA 1 cut(s) 205
BssECI CCNNGG 4 cut(s) 186, 228, 374, 985
BssMI GATC 3 cut(s) 181, 619, 777
BssNAI GTATAC 1 cut(s) 669
BssT1I CCWWGG 3 cut(s) 186, 228, 985
Bst1107I GTATAC 1 cut(s) 669
Bst2UI CCWGG 2 cut(s) 375, 704
Bst4CI ACNGT 6 cut(s) 13, 261, 335, 452, 485, 913
BstAUI TGTACA 1 cut(s) 205
BstDEI CTNAG 4 cut(s) 172, 282, 796, 829
BstKTI GATC 3 cut(s) 184, 622, 780
BstMAI GTCTC 1 cut(s) 345
BstMBI GATC 3 cut(s) 181, 619, 777
BstMWI GCNNNNNNNGC 2 cut(s) 54, 525
BstNI CCWGG 2 cut(s) 375, 704
BstSCI CCNGG 2 cut(s) 373, 702
BstV1I GCAGC 2 cut(s) 307, 329
BstV2I GAAGAC 2 cut(s) 336, 574
BstX2I RGATCY 1 cut(s) 619
BstXI CCANNNNNNTGG 1 cut(s) 710
BstYI RGATCY 1 cut(s) 619
BstZ17I GTATAC 1 cut(s) 669
BsuRI GGCC 4 cut(s) 366, 407, 528, 714
BveI ACCTGC 1 cut(s) 1052
CaiI CAGNNNCTG 1 cut(s) 899
Cfr13I GGNCC 5 cut(s) 20, 365, 475, 982, 1064
Csp6I GTAC 4 cut(s) 206, 753, 1045, 1101
CspCI CAANNNNNGTGG 2 cut(s) 943, 978
CviAII CATG 1 cut(s) 388
CviQI GTAC 4 cut(s) 206, 753, 1045, 1101
DdeI CTNAG 4 cut(s) 172, 282, 796, 829
DpnI GATC 3 cut(s) 183, 621, 779
DpnII GATC 3 cut(s) 181, 619, 777
EaeI YGGCCR 2 cut(s) 526, 712
Eco130I CCWWGG 3 cut(s) 186, 228, 985
Eco32I GATATC 1 cut(s) 401
Eco47I GGWCC 4 cut(s) 20, 475, 982, 1064
Eco57I CTGAAG 4 cut(s) 186, 608, 733, 1089
Eco88I CYCGRG 1 cut(s) 306
EcoO109I RGGNCCY 2 cut(s) 20, 1064
EcoRII CCWGG 2 cut(s) 373, 702
EcoRV GATATC 1 cut(s) 401
EcoT14I CCWWGG 3 cut(s) 186, 228, 985
ErhI CCWWGG 3 cut(s) 186, 228, 985
FaeI CATG 1 cut(s) 391
FalI AAGNNNNNCTT 2 cut(s) 1063, 1095
FatI CATG 1 cut(s) 387
FauNDI CATATG 1 cut(s) 736
FblI GTMKAC 1 cut(s) 668
Fnu4HI GCNGC 3 cut(s) 318, 321, 520
Fsp4HI GCNGC 3 cut(s) 318, 321, 520
GluI GCNGC 3 cut(s) 318, 321, 520
GsuI CTGGAG 1 cut(s) 534
HaeIII GGCC 4 cut(s) 366, 407, 528, 714
Hin1II CATG 1 cut(s) 391
HindIII AAGCTT 1 cut(s) 140
HinfI GANTC 6 cut(s) 99, 503, 531, 787, 825, 948
HphI GGTGA 1 cut(s) 650
Hpy166II GTNNAC 4 cut(s) 448, 545, 669, 1047
Hpy188I TCNGA 5 cut(s) 17, 166, 202, 627, 832
Hpy188III TCNNGA 3 cut(s) 414, 513, 1004
Hpy8I GTNNAC 4 cut(s) 448, 545, 669, 1047
HpyAV CCTTC 4 cut(s) 280, 571, 629, 737
HpyCH4III ACNGT 6 cut(s) 13, 261, 335, 452, 485, 913
HpyCH4IV ACGT 1 cut(s) 79
HpyCH4V TGCA 6 cut(s) 596, 616, 695, 917, 1061, 1140
HpyF10VI GCNNNNNNNGC 2 cut(s) 54, 525
HpyF3I CTNAG 4 cut(s) 172, 282, 796, 829
HpySE526I ACGT 1 cut(s) 79
Hsp92II CATG 1 cut(s) 391
Kzo9I GATC 3 cut(s) 181, 619, 777
LmnI GCTCC 1 cut(s) 594
Lsp1109I GCAGC 2 cut(s) 307, 329
LweI GCATC 3 cut(s) 885, 905, 1133
MaeII ACGT 1 cut(s) 79
MaeIII GTNAC 1 cut(s) 935
MalI GATC 3 cut(s) 183, 621, 779
MbiI CCGCTC 1 cut(s) 43
MboI GATC 3 cut(s) 181, 619, 777
MboII GAAGA 8 cut(s) 24, 27, 341, 574, 614, 660, 715, 1104
MfeI CAATTG 1 cut(s) 708
MflI RGATCY 1 cut(s) 619
MhlI GDGCHC 1 cut(s) 497
MlsI TGGCCA 1 cut(s) 714
MluCI AATT 9 cut(s) 196, 235, 469, 708, 990, 1020, 1026, 1030, 1165
MluNI TGGCCA 1 cut(s) 714
MlyI GAGTC 3 cut(s) 525, 781, 942
MmeI TCCRAC 5 cut(s) 92, 116, 180, 445, 925
Mox20I TGGCCA 1 cut(s) 714
MscI TGGCCA 1 cut(s) 714
MseI TTAA 2 cut(s) 1029, 1092
Msp20I TGGCCA 1 cut(s) 714
MspA1I CMGCKG 1 cut(s) 320
MspR9I CCNGG 2 cut(s) 375, 704
MunI CAATTG 1 cut(s) 708
Mva1269I GAATGC 1 cut(s) 923
MvaI CCWGG 2 cut(s) 375, 704
MwoI GCNNNNNNNGC 2 cut(s) 54, 525
NdeI CATATG 1 cut(s) 736
NdeII GATC 3 cut(s) 181, 619, 777
NlaIII CATG 1 cut(s) 391
NlaIV GGNNCC 1 cut(s) 22
PaeR7I CTCGAG 1 cut(s) 306
PcsI WCGNNNNNNNCGW 1 cut(s) 957
PctI GAATGC 1 cut(s) 923
PfeI GAWTC 3 cut(s) 99, 503, 825
PkrI GCNGC 3 cut(s) 319, 322, 521
PleI GAGTC 3 cut(s) 525, 781, 942
PpsI GAGTC 3 cut(s) 525, 781, 942
PpuMI RGGWCCY 2 cut(s) 20, 1064
PshBI ATTAAT 1 cut(s) 1029
Psp1406I AACGTT 1 cut(s) 79
Psp5II RGGWCCY 2 cut(s) 20, 1064
Psp6I CCWGG 2 cut(s) 373, 702
PspGI CCWGG 2 cut(s) 373, 702
PspN4I GGNNCC 1 cut(s) 22
PspPI GGNCC 5 cut(s) 20, 365, 475, 982, 1064
PspPPI RGGWCCY 2 cut(s) 20, 1064
PspXI VCTCGAGB 1 cut(s) 306
PstNI CAGNNNCTG 1 cut(s) 899
PsuI RGATCY 1 cut(s) 619
PvuII CAGCTG 1 cut(s) 320
RsaI GTAC 4 cut(s) 207, 754, 1046, 1102
RsaNI GTAC 4 cut(s) 206, 753, 1045, 1101
SaqAI TTAA 2 cut(s) 1029, 1092
SatI GCNGC 3 cut(s) 318, 321, 520
Sau3AI GATC 3 cut(s) 181, 619, 777
Sau96I GGNCC 5 cut(s) 20, 365, 475, 982, 1064
SchI GAGTC 3 cut(s) 525, 781, 942
ScrFI CCNGG 2 cut(s) 375, 704
SduI GDGCHC 1 cut(s) 497
SfaNI GCATC 3 cut(s) 885, 905, 1133
Sfr274I CTCGAG 1 cut(s) 306
SinI GGWCC 4 cut(s) 20, 475, 982, 1064
SlaI CTCGAG 1 cut(s) 306
SmlI CTYRAG 2 cut(s) 306, 1039
SmoI CTYRAG 2 cut(s) 306, 1039
Sse9I AATT 9 cut(s) 196, 235, 469, 708, 990, 1020, 1026, 1030, 1165
SsiI CCGC 3 cut(s) 41, 519, 664
StyD4I CCNGG 2 cut(s) 373, 702
StyI CCWWGG 3 cut(s) 186, 228, 985
TaaI ACNGT 6 cut(s) 13, 261, 335, 452, 485, 913
TaiI ACGT 1 cut(s) 82
TaqI TCGA 3 cut(s) 296, 307, 415
TasI AATT 9 cut(s) 196, 235, 469, 708, 990, 1020, 1026, 1030, 1165
TatI WGTACW 2 cut(s) 205, 1100
TauI GCSGC 1 cut(s) 522
TfiI GAWTC 3 cut(s) 99, 503, 825
Tru1I TTAA 2 cut(s) 1029, 1092
Tru9I TTAA 2 cut(s) 1029, 1092
TseI GCWGC 2 cut(s) 317, 320
TspDTI ATGAA 3 cut(s) 495, 553, 1145
TspGWI ACGGA 1 cut(s) 770
VpaK11BI GGWCC 4 cut(s) 20, 475, 982, 1064
VspI ATTAAT 1 cut(s) 1029
XapI RAATTY 3 cut(s) 196, 469, 1020
XhoI CTCGAG 1 cut(s) 306
XmiI GTMKAC 1 cut(s) 668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.