pycom13g29140

ralf-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0015943
Physical Location & Seq
Reverse (-)
25497834 .. 25498172
339 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 339 bp
ATGCAAAGGCCTAACATAGTCTTCCCTCTGATAGTCCTCGGCCTTTGCTATCTCACCCGCACAACAGCCTCAGGGATGAGCCCTTCATCCACGCCGTGCAACAGTTCGATAGTGGAGTGTGACGACGAAGGTGAGCTGCTGATGGAGTCCGAGATAAGCCGGAGGTTTCTTCAGACCAAGAAGTACATATCACCTGGGGCTTTGAAACCAGACCAACCAGTTTGCAACGGCGGCGGTAGAGGTGAAGCGTATAGTAAAACTGGAGGTTGCCTTCCTCCTCCATCTAACCCTTATAATAGAGGTTGTTCCAAGTATTATCGTTGTAGGTCGGACTCGTGA

Protein Analysis

113

Amino Acids

12.2

Weight (kDa)

8.5

Isoelectric Point (pI)

63.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 45 - 109 1.9e-25 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015943)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G14010
fragaria_vesca FvH4_4g13190
malus_domestica MD04G1018100.v1.1
prunus_persica Prupe.1G167100_v2.0.a1
pyrus_communis pycom13g29140
rosa_chinensis RchiOBHm_Chr4g0413341
rosa_multiflora Rmu_sc0007671.1_g000002 Rmu_sc0009573.1_g000009
rosa_roxburghii Rroxscaffold_5G00357260
rosa_rugosa Rorug04G0120000
rosa_samantha Rh4AG179400 Rh4BG178500 Rh4CG190000 Rh4DG174500
rosa_wichuraiana Rw4G014910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 294
AciI CCGC 3 cut(s) 58, 231, 234
AcuI CTGAAG 1 cut(s) 155
AdeI CACNNNGTG 1 cut(s) 96
AfaI GTAC 1 cut(s) 185
AgsI TTSAA 1 cut(s) 205
AjnI CCWGG 1 cut(s) 193
AluBI AGCT 1 cut(s) 136
AluI AGCT 1 cut(s) 136
AoxI GGCC 2 cut(s) 8, 40
ApeKI GCWGC 1 cut(s) 136
AsuHPI GGTGA 4 cut(s) 46, 143, 183, 254
AxyI CCTNAGG 1 cut(s) 70
BanII GRGCYC 1 cut(s) 83
BauI CACGAG 1 cut(s) 334
BbsI GAAGAC 1 cut(s) 13
BbvI GCAGC 1 cut(s) 123
BccI CCATC 2 cut(s) 136, 289
BceAI ACGGC 2 cut(s) 79, 244
BciT130I CCWGG 1 cut(s) 195
BisI GCNGC 2 cut(s) 137, 232
BlsI GCNGC 2 cut(s) 138, 233
Bme1390I CCNGG 1 cut(s) 195
BmrFI CCNGG 1 cut(s) 195
BpiI GAAGAC 1 cut(s) 13
BpmI CTGGAG 1 cut(s) 282
BsaJI CCNNGG 2 cut(s) 37, 194
Bse1I ACTGG 2 cut(s) 218, 265
Bse21I CCTNAGG 1 cut(s) 70
BseBI CCWGG 1 cut(s) 195
BseDI CCNNGG 2 cut(s) 37, 194
BseGI GGATG 2 cut(s) 81, 86
BseMII CTCAG 1 cut(s) 84
BseNI ACTGG 2 cut(s) 218, 265
BseRI GAGGAG 1 cut(s) 267
BseXI GCAGC 1 cut(s) 123
BshFI GGCC 2 cut(s) 10, 42
BsiSI CCGG 1 cut(s) 160
BsnI GGCC 2 cut(s) 10, 42
Bsp1286I GDGCHC 1 cut(s) 83
BspACI CCGC 3 cut(s) 58, 231, 234
BspANI GGCC 2 cut(s) 10, 42
BspCNI CTCAG 1 cut(s) 83
BsrI ACTGG 2 cut(s) 218, 265
BssECI CCNNGG 2 cut(s) 37, 194
BssSI CACGAG 1 cut(s) 334
Bst2BI CACGAG 1 cut(s) 334
Bst2UI CCWGG 1 cut(s) 195
Bst4CI ACNGT 1 cut(s) 104
BstDEI CTNAG 1 cut(s) 70
BstF5I GGATG 2 cut(s) 81, 86
BstMWI GCNNNNNNNGC 1 cut(s) 231
BstNI CCWGG 1 cut(s) 195
BstSCI CCNGG 1 cut(s) 193
BstV1I GCAGC 1 cut(s) 123
BstV2I GAAGAC 1 cut(s) 13
Bsu36I CCTNAGG 1 cut(s) 70
BsuRI GGCC 2 cut(s) 10, 42
BtsCI GGATG 2 cut(s) 81, 86
Csp6I GTAC 1 cut(s) 184
CviJI RGCY 7 cut(s) 10, 42, 68, 81, 136, 159, 200
CviKI_1 RGCY 7 cut(s) 10, 42, 68, 81, 136, 159, 200
CviQI GTAC 1 cut(s) 184
DdeI CTNAG 1 cut(s) 70
DraIII CACNNNGTG 1 cut(s) 96
Eco147I AGGCCT 1 cut(s) 10
Eco24I GRGCYC 1 cut(s) 83
Eco57I CTGAAG 1 cut(s) 155
Eco81I CCTNAGG 1 cut(s) 70
EcoRII CCWGG 1 cut(s) 193
EcoT38I GRGCYC 1 cut(s) 83
FaiI YATR 4 cut(s) 17, 188, 252, 294
FauI CCCGC 1 cut(s) 65
Fnu4HI GCNGC 2 cut(s) 137, 232
FokI GGATG 2 cut(s) 73, 88
FriOI GRGCYC 1 cut(s) 83
Fsp4HI GCNGC 2 cut(s) 137, 232
GluI GCNGC 2 cut(s) 137, 232
GsuI CTGGAG 1 cut(s) 282
HaeIII GGCC 2 cut(s) 10, 42
HapII CCGG 1 cut(s) 160
HinfI GANTC 2 cut(s) 146, 332
HpaII CCGG 1 cut(s) 160
HphI GGTGA 4 cut(s) 46, 143, 183, 254
Hpy188I TCNGA 4 cut(s) 30, 151, 174, 331
Hpy188III TCNNGA 1 cut(s) 336
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 3 cut(s) 93, 122, 281
HpyCH4III ACNGT 1 cut(s) 104
HpyCH4V TGCA 3 cut(s) 4, 99, 225
HpyF10VI GCNNNNNNNGC 1 cut(s) 231
HpyF3I CTNAG 1 cut(s) 70
LpnPI CCDG 7 cut(s) 57, 173, 180, 207, 222, 231, 246
Lsp1109I GCAGC 1 cut(s) 123
MaeIII GTNAC 1 cut(s) 119
MboII GAAGA 2 cut(s) 13, 161
MhlI GDGCHC 1 cut(s) 83
MlyI GAGTC 2 cut(s) 155, 326
MmeI TCCRAC 1 cut(s) 309
MnlI CCTC 9 cut(s) 36, 47, 79, 156, 233, 257, 285, 288, 293
MspI CCGG 1 cut(s) 160
MspR9I CCNGG 1 cut(s) 195
MvaI CCWGG 1 cut(s) 195
MwoI GCNNNNNNNGC 1 cut(s) 231
NmeAIII GCCGAG 1 cut(s) 18
NmuCI GTSAC 1 cut(s) 119
PceI AGGCCT 1 cut(s) 10
PkrI GCNGC 2 cut(s) 138, 233
PleI GAGTC 2 cut(s) 154, 326
PpsI GAGTC 2 cut(s) 154, 326
PsiI TTATAA 1 cut(s) 294
Psp6I CCWGG 1 cut(s) 193
PspGI CCWGG 1 cut(s) 193
RsaI GTAC 1 cut(s) 185
RsaNI GTAC 1 cut(s) 184
SatI GCNGC 2 cut(s) 137, 232
SchI GAGTC 2 cut(s) 155, 326
ScrFI CCNGG 1 cut(s) 195
SduI GDGCHC 1 cut(s) 83
SetI ASST 8 cut(s) 133, 138, 167, 196, 244, 268, 304, 329
SseBI AGGCCT 1 cut(s) 10
SsiI CCGC 3 cut(s) 58, 231, 234
StuI AGGCCT 1 cut(s) 10
StyD4I CCNGG 1 cut(s) 193
TaaI ACNGT 1 cut(s) 104
TaqI TCGA 1 cut(s) 107
TatI WGTACW 1 cut(s) 183
TauI GCSGC 1 cut(s) 234
TseFI GTSAC 1 cut(s) 119
TseI GCWGC 1 cut(s) 136
Tsp45I GTSAC 1 cut(s) 119
TspDTI ATGAA 1 cut(s) 75
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.