pycom14g00280

Lysosomal beta glucosidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0019805
Physical Location & Seq
Reverse (-)
159940 .. 160612
673 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 432 bp
ATGCCAGGCTACTATGACTCAATTATCAAGGGTGTGGCAACCATTATGGTATCCTACTCAAGCTGGAACGGAGTAAAGATGCACGTTAATCGTGATCTTATCACTGGCTTCCTTAAGAACACTCTTCGATTCAGGGGTTTTGTCATCTCAGATTGGGAGGGTATTGATAGGATCACCTCTCCACCTCATGCCAACTACACATATTCGATTCAAGCAGGAATCAGTGCCGGCATTGATATGGTCATGGTTCCATACAAGTATACGGAATTCATTAACGATCTAACCTTCCTGAGAATTTTGCGAGTTAAGTTTGTGATGGGCCTATTTGAGGAACCATTGGCTGATCTCAGCTTTATCAATCAGCTCGGCTGCCAGGTAACTATTGGTAAACTTGACAACATTCTACAAGCACTGAGGAAAACTTTAATGTAA

Protein Analysis

144

Amino Acids

16.25

Weight (kDa)

8.71

Isoelectric Point (pI)

37.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_3 PF00933 1 - 94 6.4e-14 Glycosyl hydrolase family 3 N terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019805)

Species Orthologous Gene IDs
prunus_persica Prupe.7G006800_v2.0.a1
pyrus_communis pycom14g00280
rosa_chinensis RchiOBHm_Chr5g0049451
rosa_multiflora Rmu_co8185098.1_g000001
rosa_samantha Rh5BG336100 Rh5BG336200 Rh5DG348400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 260
AclWI GGATC 1 cut(s) 179
AcsI RAATTY 2 cut(s) 266, 294
AfiI CCNNNNNNNGG 1 cut(s) 328
AflII CTTAAG 1 cut(s) 113
AgsI TTSAA 1 cut(s) 212
AjnI CCWGG 2 cut(s) 4, 372
AluBI AGCT 3 cut(s) 63, 351, 364
AluI AGCT 3 cut(s) 63, 351, 364
AlwI GGATC 1 cut(s) 179
AoxI GGCC 1 cut(s) 319
ApeKI GCWGC 1 cut(s) 369
ApoI RAATTY 2 cut(s) 266, 294
AspS9I GGNCC 1 cut(s) 319
AsuHPI GGTGA 1 cut(s) 166
BbvI GCAGC 1 cut(s) 356
BccI CCATC 1 cut(s) 310
BcgI CGANNNNNNTGC 2 cut(s) 71, 105
BciT130I CCWGG 2 cut(s) 6, 374
BciVI GTATCC 1 cut(s) 61
BfrI CTTAAG 1 cut(s) 113
BfuI GTATCC 1 cut(s) 61
BisI GCNGC 1 cut(s) 370
BlsI GCNGC 1 cut(s) 371
Bme1390I CCNGG 2 cut(s) 6, 374
BmgT120I GGNCC 1 cut(s) 319
BmiI GGNNCC 2 cut(s) 249, 333
BmrFI CCNGG 2 cut(s) 6, 374
BmsI GCATC 1 cut(s) 69
BpuEI CTTGAG 1 cut(s) 43
Bsc4I CCNNNNNNNGG 1 cut(s) 328
Bse118I RCCGGY 1 cut(s) 227
Bse1I ACTGG 1 cut(s) 109
BseBI CCWGG 2 cut(s) 6, 374
BseLI CCNNNNNNNGG 1 cut(s) 328
BseMII CTCAG 4 cut(s) 162, 281, 361, 404
BseNI ACTGG 1 cut(s) 109
BseXI GCAGC 1 cut(s) 356
BshFI GGCC 1 cut(s) 321
BsiSI CCGG 1 cut(s) 228
BslI CCNNNNNNNGG 1 cut(s) 328
BsnI GGCC 1 cut(s) 321
Bsp143I GATC 4 cut(s) 94, 171, 277, 343
BspANI GGCC 1 cut(s) 321
BspCNI CTCAG 4 cut(s) 161, 282, 360, 405
BspLI GGNNCC 2 cut(s) 249, 333
BspPI GGATC 1 cut(s) 179
BspTI CTTAAG 1 cut(s) 113
BsrFI RCCGGY 1 cut(s) 227
BsrI ACTGG 1 cut(s) 109
BssAI RCCGGY 1 cut(s) 227
BssMI GATC 4 cut(s) 94, 171, 277, 343
BssNAI GTATAC 1 cut(s) 261
Bst1107I GTATAC 1 cut(s) 261
Bst2UI CCWGG 2 cut(s) 6, 374
Bst6I CTCTTC 1 cut(s) 129
BstAFI CTTAAG 1 cut(s) 113
BstC8I GCNNGC 1 cut(s) 229
BstDEI CTNAG 4 cut(s) 148, 290, 347, 413
BstENI CCTNNNNNAGG 1 cut(s) 326
BstKTI GATC 4 cut(s) 97, 174, 280, 346
BstMBI GATC 4 cut(s) 94, 171, 277, 343
BstNI CCWGG 2 cut(s) 6, 374
BstSCI CCNGG 2 cut(s) 4, 372
BstV1I GCAGC 1 cut(s) 356
BstZ17I GTATAC 1 cut(s) 261
BsuI GTATCC 1 cut(s) 61
BsuRI GGCC 1 cut(s) 321
BtsIMutI CAGTG 3 cut(s) 102, 229, 410
Cac8I GCNNGC 1 cut(s) 229
Cfr10I RCCGGY 1 cut(s) 227
Cfr13I GGNCC 1 cut(s) 319
CviAII CATG 2 cut(s) 188, 244
CviJI RGCY 8 cut(s) 9, 63, 108, 321, 341, 351, 364, 369
CviKI_1 RGCY 8 cut(s) 9, 63, 108, 321, 341, 351, 364, 369
DdeI CTNAG 4 cut(s) 148, 290, 347, 413
DpnI GATC 4 cut(s) 96, 173, 279, 345
DpnII GATC 4 cut(s) 94, 171, 277, 343
Eam1104I CTCTTC 1 cut(s) 129
EarI CTCTTC 1 cut(s) 129
EcoNI CCTNNNNNAGG 1 cut(s) 326
EcoRI GAATTC 1 cut(s) 266
EcoRII CCWGG 2 cut(s) 4, 372
FaeI CATG 2 cut(s) 191, 247
FaiI YATR 8 cut(s) 15, 47, 189, 202, 239, 245, 253, 261
FatI CATG 2 cut(s) 187, 243
FblI GTMKAC 1 cut(s) 260
Fnu4HI GCNGC 1 cut(s) 370
Fsp4HI GCNGC 1 cut(s) 370
GluI GCNGC 1 cut(s) 370
HaeIII GGCC 1 cut(s) 321
HapII CCGG 1 cut(s) 228
Hin1II CATG 2 cut(s) 191, 247
HinfI GANTC 4 cut(s) 17, 129, 208, 219
HpaII CCGG 1 cut(s) 228
HphI GGTGA 1 cut(s) 166
Hpy166II GTNNAC 2 cut(s) 261, 389
Hpy188I TCNGA 1 cut(s) 151
Hpy188III TCNNGA 2 cut(s) 92, 289
Hpy8I GTNNAC 2 cut(s) 261, 389
HpyAV CCTTC 1 cut(s) 295
HpyCH4IV ACGT 1 cut(s) 84
HpyCH4V TGCA 1 cut(s) 82
HpyF3I CTNAG 4 cut(s) 148, 290, 347, 413
HpySE526I ACGT 1 cut(s) 84
Hsp92II CATG 2 cut(s) 191, 247
KroI GCCGGC 1 cut(s) 227
KroNI GCCGGC 1 cut(s) 229
Kzo9I GATC 4 cut(s) 94, 171, 277, 343
LpnPI CCDG 9 cut(s) 18, 49, 90, 118, 201, 241, 302, 359, 386
Lsp1109I GCAGC 1 cut(s) 356
LweI GCATC 1 cut(s) 69
MaeII ACGT 1 cut(s) 84
MaeIII GTNAC 1 cut(s) 376
MalI GATC 4 cut(s) 96, 173, 279, 345
MboI GATC 4 cut(s) 94, 171, 277, 343
MboII GAAGA 1 cut(s) 116
MluCI AATT 3 cut(s) 21, 266, 294
MlyI GAGTC 1 cut(s) 11
MnlI CCTC 5 cut(s) 151, 187, 195, 322, 408
MroNI GCCGGC 1 cut(s) 227
MseI TTAA 5 cut(s) 87, 114, 273, 306, 425
MslI CAYNNNNRTG 1 cut(s) 236
MspCI CTTAAG 1 cut(s) 113
MspI CCGG 1 cut(s) 228
MspR9I CCNGG 2 cut(s) 6, 374
MvaI CCWGG 2 cut(s) 6, 374
NaeI GCCGGC 1 cut(s) 229
NdeII GATC 4 cut(s) 94, 171, 277, 343
NgoMIV GCCGGC 1 cut(s) 227
NlaIII CATG 2 cut(s) 191, 247
NlaIV GGNNCC 2 cut(s) 249, 333
NmeAIII GCCGAG 1 cut(s) 345
PdiI GCCGGC 1 cut(s) 229
PfeI GAWTC 3 cut(s) 129, 208, 219
PkrI GCNGC 1 cut(s) 371
PleI GAGTC 1 cut(s) 11
PpsI GAGTC 1 cut(s) 11
Psp6I CCWGG 2 cut(s) 4, 372
PspGI CCWGG 2 cut(s) 4, 372
PspN4I GGNNCC 2 cut(s) 249, 333
PspPI GGNCC 1 cut(s) 319
RseI CAYNNNNRTG 1 cut(s) 236
SaqAI TTAA 5 cut(s) 87, 114, 273, 306, 425
SatI GCNGC 1 cut(s) 370
Sau3AI GATC 4 cut(s) 94, 171, 277, 343
Sau96I GGNCC 1 cut(s) 319
SchI GAGTC 1 cut(s) 11
ScrFI CCNGG 2 cut(s) 6, 374
SetI ASST 8 cut(s) 65, 87, 179, 187, 287, 353, 366, 378
SfaNI GCATC 1 cut(s) 69
SmiMI CAYNNNNRTG 1 cut(s) 236
SmlI CTYRAG 2 cut(s) 58, 113
SmoI CTYRAG 2 cut(s) 58, 113
Sse9I AATT 3 cut(s) 21, 266, 294
StyD4I CCNGG 2 cut(s) 4, 372
TaiI ACGT 1 cut(s) 87
TaqI TCGA 2 cut(s) 127, 206
TasI AATT 3 cut(s) 21, 266, 294
TfiI GAWTC 3 cut(s) 129, 208, 219
Tru1I TTAA 5 cut(s) 87, 114, 273, 306, 425
Tru9I TTAA 5 cut(s) 87, 114, 273, 306, 425
TscAI CASTG 3 cut(s) 109, 229, 417
TseI GCWGC 1 cut(s) 369
TspDTI ATGAA 1 cut(s) 259
TspGWI ACGGA 2 cut(s) 84, 278
TspRI CASTG 3 cut(s) 109, 229, 417
Vha464I CTTAAG 1 cut(s) 113
XagI CCTNNNNNAGG 1 cut(s) 326
XapI RAATTY 2 cut(s) 266, 294
XcmI CCANNNNNNNNNTGG 1 cut(s) 380
XmiI GTMKAC 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.