Rh5BG336100

Lysosomal beta glucosidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0019805
Physical Location & Seq
Reverse (-)
46847749 .. 46864390
16642 bp
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UTR
Exon/CDS
Intron
Rh5BG336100.1

Sequence Viewer

Length: 561 bp
ATGGCGTTATCTAATTTGCATAACAATTTTGCCATCTTGTGCAGGGATCCAGAACTTGTGAAGAAAATTGGTTCTGCAACTGCTCTCGTAGTTAGAGCTGCTGGCATTAAGCCATTAACTATGCTTTTTCACCATGTGTTGCGGTCTGCAGAGATCCAAGATGGGGTAGATGTATGTTACGAGAGTTACAGCGAGGATCCTGAAATCGTAAAACAAATGACTGATATCATACTTCGATTGCAAGGCGAAATACCAGCTGATTCACGGAAGGGTGTTCCATTTGTTGGTGGAAAGGATAAGGTAGCGGCTTGCGCAAAACACTTTGTTGGAGACAGTGGTACGATTAAGGGCATCAATGAGAACGATACCGTGATTGATAAGCATGGATTATTGAACATTCACATGCCTGGTTATTACCATTCTATCATCAAGGGCGTATCCACTGTCATGGTTTCCTACTCCAGCTTGAATGGAACGAAGATGCATGCTAACCACAAGCTCATCGCCCAATTTCTTAAGAACACGCTTAAGTTCAGGGTACGTATGTATACAACAGCCTAA

Protein Analysis

186

Amino Acids

20.48

Weight (kDa)

9.1

Isoelectric Point (pI)

36.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_3 PF00933 15 - 178 3e-18 Glycosyl hydrolase family 3 N terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0019805)

Species Orthologous Gene IDs
prunus_persica Prupe.7G006800_v2.0.a1
pyrus_communis pycom14g00280
rosa_chinensis RchiOBHm_Chr5g0049451
rosa_multiflora Rmu_co8185098.1_g000001
rosa_samantha Rh5BG336100 Rh5BG336200 Rh5DG348400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 313
AccB7I CCANNNNNTGG 1 cut(s) 284
AccI GTMKAC 1 cut(s) 548
AciI CCGC 2 cut(s) 142, 305
AclWI GGATC 5 cut(s) 41, 54, 148, 191, 204
AdeI CACNNNGTG 1 cut(s) 136
AfaI GTAC 2 cut(s) 340, 540
AfiI CCNNNNNNNGG 2 cut(s) 163, 284
AflII CTTAAG 2 cut(s) 515, 527
AgsI TTSAA 2 cut(s) 394, 469
AjnI CCWGG 1 cut(s) 406
AluBI AGCT 4 cut(s) 98, 257, 465, 499
AluI AGCT 4 cut(s) 98, 257, 465, 499
Alw26I GTCTC 1 cut(s) 324
AlwI GGATC 5 cut(s) 41, 54, 148, 191, 204
ApeKI GCWGC 1 cut(s) 98
AspLEI GCGC 1 cut(s) 314
AsuHPI GGTGA 1 cut(s) 122
BaeI ACNNNNGTAYC 1 cut(s) 530
BamHI GGATCC 2 cut(s) 46, 196
BbvI GCAGC 1 cut(s) 85
BccI CCATC 2 cut(s) 41, 155
BciT130I CCWGG 1 cut(s) 408
BciVI GTATCC 1 cut(s) 448
BcoDI GTCTC 1 cut(s) 324
BfmI CTRYAG 1 cut(s) 147
BfrI CTTAAG 2 cut(s) 515, 527
BfuI GTATCC 1 cut(s) 448
BisI GCNGC 2 cut(s) 99, 306
BlsI GCNGC 2 cut(s) 100, 307
Bme1390I CCNGG 1 cut(s) 408
BmiI GGNNCC 2 cut(s) 48, 198
BmrFI CCNGG 1 cut(s) 408
BmsI GCATC 2 cut(s) 360, 471
BpmI CTGGAG 1 cut(s) 445
BsaAI YACGTR 1 cut(s) 542
Bsc4I CCNNNNNNNGG 2 cut(s) 163, 284
BseBI CCWGG 1 cut(s) 408
BseLI CCNNNNNNNGG 2 cut(s) 163, 284
BseXI GCAGC 1 cut(s) 85
BsgI GTGCAG 1 cut(s) 61
BslI CCNNNNNNNGG 2 cut(s) 163, 284
BsmAI GTCTC 1 cut(s) 324
Bsp143I GATC 3 cut(s) 46, 153, 196
BspACI CCGC 2 cut(s) 142, 305
BspLI GGNNCC 2 cut(s) 48, 198
BspMAI CTGCAG 1 cut(s) 151
BspPI GGATC 5 cut(s) 41, 54, 148, 191, 204
BspTI CTTAAG 2 cut(s) 515, 527
BssMI GATC 3 cut(s) 46, 153, 196
BssNAI GTATAC 1 cut(s) 549
Bst1107I GTATAC 1 cut(s) 549
Bst2UI CCWGG 1 cut(s) 408
Bst4CI ACNGT 3 cut(s) 335, 370, 445
BstAFI CTTAAG 2 cut(s) 515, 527
BstBAI YACGTR 1 cut(s) 542
BstC8I GCNNGC 3 cut(s) 103, 310, 486
BstHHI GCGC 1 cut(s) 314
BstKTI GATC 3 cut(s) 49, 156, 199
BstMAI GTCTC 1 cut(s) 324
BstMBI GATC 3 cut(s) 46, 153, 196
BstMWI GCNNNNNNNGC 1 cut(s) 311
BstNI CCWGG 1 cut(s) 408
BstNSI RCATGY 2 cut(s) 406, 488
BstSCI CCNGG 1 cut(s) 406
BstSFI CTRYAG 1 cut(s) 147
BstSNI TACGTA 1 cut(s) 542
BstV1I GCAGC 1 cut(s) 85
BstX2I RGATCY 3 cut(s) 46, 153, 196
BstXI CCANNNNNNTGG 1 cut(s) 448
BstYI RGATCY 3 cut(s) 46, 153, 196
BstZ17I GTATAC 1 cut(s) 549
BsuI GTATCC 1 cut(s) 448
BtgZI GCGATG 1 cut(s) 487
BtsIMutI CAGTG 2 cut(s) 340, 441
Cac8I GCNNGC 3 cut(s) 103, 310, 486
CfoI GCGC 1 cut(s) 314
Csp6I GTAC 2 cut(s) 339, 539
CviAII CATG 5 cut(s) 134, 383, 403, 448, 485
CviJI RGCY 7 cut(s) 98, 112, 257, 308, 465, 499, 557
CviKI_1 RGCY 7 cut(s) 98, 112, 257, 308, 465, 499, 557
CviQI GTAC 2 cut(s) 339, 539
DpnI GATC 3 cut(s) 48, 155, 198
DpnII GATC 3 cut(s) 46, 153, 196
DraIII CACNNNGTG 1 cut(s) 136
Eco105I TACGTA 1 cut(s) 542
Eco32I GATATC 1 cut(s) 226
EcoRII CCWGG 1 cut(s) 406
EcoRV GATATC 1 cut(s) 226
EcoT22I ATGCAT 1 cut(s) 486
FaeI CATG 5 cut(s) 137, 386, 406, 451, 488
FatI CATG 5 cut(s) 133, 382, 402, 447, 484
FblI GTMKAC 1 cut(s) 548
Fnu4HI GCNGC 2 cut(s) 99, 306
Fsp4HI GCNGC 2 cut(s) 99, 306
FspI TGCGCA 1 cut(s) 313
GlaI GCGC 1 cut(s) 313
GluI GCNGC 2 cut(s) 99, 306
GsuI CTGGAG 1 cut(s) 445
HhaI GCGC 1 cut(s) 314
Hin1II CATG 5 cut(s) 137, 386, 406, 451, 488
Hin6I GCGC 1 cut(s) 312
HinP1I GCGC 1 cut(s) 312
HinfI GANTC 1 cut(s) 260
HphI GGTGA 1 cut(s) 122
Hpy166II GTNNAC 1 cut(s) 549
Hpy188III TCNNGA 2 cut(s) 50, 200
Hpy8I GTNNAC 1 cut(s) 549
HpyAV CCTTC 1 cut(s) 262
HpyCH4III ACNGT 3 cut(s) 335, 370, 445
HpyCH4IV ACGT 1 cut(s) 541
HpyCH4V TGCA 6 cut(s) 19, 42, 77, 149, 241, 484
HpyF10VI GCNNNNNNNGC 1 cut(s) 311
HpySE526I ACGT 1 cut(s) 541
Hsp92II CATG 5 cut(s) 137, 386, 406, 451, 488
HspAI GCGC 1 cut(s) 312
Kzo9I GATC 3 cut(s) 46, 153, 196
LpnPI CCDG 9 cut(s) 28, 63, 87, 213, 267, 393, 420, 475, 520
Lsp1109I GCAGC 1 cut(s) 85
LweI GCATC 2 cut(s) 360, 471
MaeII ACGT 1 cut(s) 541
MaeIII GTNAC 2 cut(s) 176, 185
MalI GATC 3 cut(s) 48, 155, 198
MboI GATC 3 cut(s) 46, 153, 196
MboII GAAGA 2 cut(s) 73, 490
MflI RGATCY 3 cut(s) 46, 153, 196
MluCI AATT 4 cut(s) 13, 25, 66, 509
MmeI TCCRAC 1 cut(s) 307
MnlI CCTC 1 cut(s) 187
Mph1103I ATGCAT 1 cut(s) 486
MseI TTAA 5 cut(s) 108, 116, 345, 516, 528
MslI CAYNNNNRTG 2 cut(s) 401, 446
MspA1I CMGCKG 1 cut(s) 257
MspCI CTTAAG 2 cut(s) 515, 527
MspR9I CCNGG 1 cut(s) 408
MvaI CCWGG 1 cut(s) 408
MwoI GCNNNNNNNGC 1 cut(s) 311
NdeII GATC 3 cut(s) 46, 153, 196
NlaIII CATG 5 cut(s) 137, 386, 406, 451, 488
NlaIV GGNNCC 2 cut(s) 48, 198
NsbI TGCGCA 1 cut(s) 313
NsiI ATGCAT 1 cut(s) 486
NspI RCATGY 2 cut(s) 406, 488
PaeI GCATGC 1 cut(s) 488
PfeI GAWTC 1 cut(s) 260
PflMI CCANNNNNTGG 1 cut(s) 284
PkrI GCNGC 2 cut(s) 100, 307
Ppu21I YACGTR 1 cut(s) 542
Psp6I CCWGG 1 cut(s) 406
PspGI CCWGG 1 cut(s) 406
PspN4I GGNNCC 2 cut(s) 48, 198
PstI CTGCAG 1 cut(s) 151
PsuI RGATCY 3 cut(s) 46, 153, 196
PvuII CAGCTG 1 cut(s) 257
RsaI GTAC 2 cut(s) 340, 540
RsaNI GTAC 2 cut(s) 339, 539
RseI CAYNNNNRTG 2 cut(s) 401, 446
SaqAI TTAA 5 cut(s) 108, 116, 345, 516, 528
SatI GCNGC 2 cut(s) 99, 306
Sau3AI GATC 3 cut(s) 46, 153, 196
ScrFI CCNGG 1 cut(s) 408
SetI ASST 6 cut(s) 100, 259, 303, 467, 501, 544
SfaNI GCATC 2 cut(s) 360, 471
SfcI CTRYAG 1 cut(s) 147
SmiMI CAYNNNNRTG 2 cut(s) 401, 446
SmlI CTYRAG 2 cut(s) 515, 527
SmoI CTYRAG 2 cut(s) 515, 527
SnaBI TACGTA 1 cut(s) 542
SphI GCATGC 1 cut(s) 488
Sse9I AATT 4 cut(s) 13, 25, 66, 509
SsiI CCGC 2 cut(s) 142, 305
StyD4I CCNGG 1 cut(s) 406
TaaI ACNGT 3 cut(s) 335, 370, 445
TaiI ACGT 1 cut(s) 544
TaqI TCGA 1 cut(s) 235
TasI AATT 4 cut(s) 13, 25, 66, 509
TauI GCSGC 1 cut(s) 308
TfiI GAWTC 1 cut(s) 260
Tru1I TTAA 5 cut(s) 108, 116, 345, 516, 528
Tru9I TTAA 5 cut(s) 108, 116, 345, 516, 528
TscAI CASTG 2 cut(s) 340, 448
TseI GCWGC 1 cut(s) 98
TspGWI ACGGA 1 cut(s) 280
TspRI CASTG 2 cut(s) 340, 448
Van91I CCANNNNNTGG 1 cut(s) 284
Vha464I CTTAAG 2 cut(s) 515, 527
XceI RCATGY 2 cut(s) 406, 488
XmiI GTMKAC 1 cut(s) 548
Zsp2I ATGCAT 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.