pycom14g00550

zinc metalloprotease EGY1, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0011472
Physical Location & Seq
Reverse (-)
371967 .. 374881
2915 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1035 bp
ATGGTGGAGGAACCCAATGCAGAAGATCTAGATCCACGTGGTGGTCCACGTGTTAGCTTTGGCTTGCTGCGGAAAGAGGTCTCAGAACCAGGGCCAACCACACTTTGGCAATATGTAATTGCTTTCTTGTTATTCCTTCTAACAATTGGATCATCTGTGGAGTTAGGAATTGCATCTCAGATAAATAAACTTCCGCCAGAGGTGGTGAAATACTTCACAGACCCAAATGCCGTTGACCCACCAGATATGGAGTTGCTATCCCCGTTTGTAGATTCCGCTTTGCCCTTAGCATATGGTGTTCTGGGGGTTTTGTTGTTTCATGAAGTTGGGCATTTTTTGGCTGCTTTCCCAAACAAAGTAAAACTTAGCATTCCTTTCTTCATTCCTAATATTACTCTTGGTAGCTTTGGCGCCATCACTCAGTTTAAATCCATTCTCCCTGATCGGAGCACAAAAGTCGACATATCACTGGCGGGTCCTTTTGCCGGTGCTGCGTTGTCATTGTCAATGTTTGCTGTTGGCCTGCTGCTCTCATCTAATCCCAATGCCACAGGAGACTTGGTGCAGGTTCCAAGCACGCTGTTTCAAGGTTCTTTGCTTCTTGGACTCATCAGTAGAGCCACTCTTGGTTATGCATCAATGCATGCTGCGACTGTTCCAATTCATCCTCTTATGATTGCTGGCTGGTGTGGGTTGACAACAACAGCTTTTAATATGCTTCCAGTGGGCTGTCTTGATGGTGGAAGAGCTGTGCAGGGCGCTTTTGGAAAAAATGCGCTCGTTGCCTTTGGCTTGACGACATACACCATGCTTGGATTGGGGGTGCTTGGCGGACCTCTGTCACTTCCATGGGGACTCTACGTGCTCATATGCCAGAGGACTCCCGAGAAACCGTGTCTGAATGATGTAACAGAGGTTGGAACGTGGAGGAAAACAATTGTTACAGCGGCCGTGTTCCTTGTTGTACTCACACTTCTTCCTGTATGGGATGAGCTTGCCGAGGAGCTAGGTATAGGTCTTGTAAGCACATTTTGA

Protein Analysis

345

Amino Acids

36.51

Weight (kDa)

4.99

Isoelectric Point (pI)

28.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M50 PF02163 97 - 256 4.7e-19 Peptidase family M50
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 556
AccB1I GGYRCC 1 cut(s) 410
AccB7I CCANNNNNTGG 2 cut(s) 41, 105
AccI GTMKAC 1 cut(s) 459
AciI CCGC 6 cut(s) 70, 194, 276, 473, 831, 947
AclWI GGATC 2 cut(s) 26, 157
AcoI YGGCCR 1 cut(s) 948
AcvI CACGTG 2 cut(s) 38, 50
AcyI GRCGYC 1 cut(s) 411
AdeI CACNNNGTG 1 cut(s) 41
AfaI GTAC 1 cut(s) 966
AfiI CCNNNNNNNGG 3 cut(s) 41, 105, 485
AflIII ACRYGT 1 cut(s) 49
AgsI TTSAA 1 cut(s) 587
AjnI CCWGG 1 cut(s) 88
AluBI AGCT 6 cut(s) 57, 405, 707, 749, 994, 1006
AluI AGCT 6 cut(s) 57, 405, 707, 749, 994, 1006
Alw21I GWGCWC 2 cut(s) 452, 867
Alw26I GTCTC 2 cut(s) 85, 549
AlwI GGATC 2 cut(s) 26, 157
Ama87I CYCGRG 1 cut(s) 884
AoxI GGCC 3 cut(s) 92, 520, 948
ApeKI GCWGC 5 cut(s) 67, 341, 491, 526, 647
Asp700I GAANNNNTTC 1 cut(s) 212
AspLEI GCGC 3 cut(s) 413, 761, 778
AspS9I GGNCC 4 cut(s) 44, 92, 476, 833
AsuHPI GGTGA 1 cut(s) 217
AvaI CYCGRG 1 cut(s) 884
AvaII GGWCC 3 cut(s) 44, 476, 833
BanI GGYRCC 1 cut(s) 410
BarI GAAGNNNNNNTAC 2 cut(s) 957, 989
BbrPI CACGTG 2 cut(s) 38, 50
Bbv12I GWGCWC 2 cut(s) 452, 867
BbvI GCAGC 5 cut(s) 54, 328, 478, 513, 634
BccI CCATC 2 cut(s) 422, 731
BceAI ACGGC 2 cut(s) 215, 935
BcgI CGANNNNNNTGC 2 cut(s) 439, 473
BciT130I CCWGG 1 cut(s) 90
BcoDI GTCTC 2 cut(s) 85, 549
BfaI CTAG 2 cut(s) 29, 1007
BfoI RGCGCY 2 cut(s) 414, 762
BfuAI ACCTGC 1 cut(s) 556
BglII AGATCT 1 cut(s) 25
BisI GCNGC 6 cut(s) 68, 342, 492, 527, 648, 948
BlsI GCNGC 6 cut(s) 69, 343, 493, 528, 649, 949
Bme1390I CCNGG 1 cut(s) 90
Bme18I GGWCC 3 cut(s) 44, 476, 833
BmeT110I CYCGRG 1 cut(s) 884
BmgT120I GGNCC 4 cut(s) 44, 92, 476, 833
BmiI GGNNCC 4 cut(s) 12, 412, 477, 570
BmrFI CCNGG 1 cut(s) 90
BmsI GCATC 2 cut(s) 182, 644
BoxI GACNNNNGTC 1 cut(s) 838
Bpu10I CCTNAGC 1 cut(s) 286
BsaAI YACGTR 3 cut(s) 38, 50, 862
BsaBI GATNNNNATC 1 cut(s) 30
BsaHI GRCGYC 1 cut(s) 411
BsaI GGTCTC 1 cut(s) 85
BsaJI CCNNGG 3 cut(s) 89, 848, 999
BsaXI ACNNNNNCTCC 2 cut(s) 546, 576
Bsc4I CCNNNNNNNGG 3 cut(s) 41, 105, 485
Bse118I RCCGGY 1 cut(s) 485
Bse1I ACTGG 2 cut(s) 474, 722
Bse8I GATNNNNATC 1 cut(s) 30
BseBI CCWGG 1 cut(s) 90
BseDI CCNNGG 3 cut(s) 89, 848, 999
BseGI GGATG 2 cut(s) 664, 994
BseJI GATNNNNATC 1 cut(s) 30
BseLI CCNNNNNNNGG 3 cut(s) 41, 105, 485
BseMII CTCAG 3 cut(s) 96, 191, 434
BseNI ACTGG 2 cut(s) 474, 722
BseRI GAGGAG 1 cut(s) 1016
BseX3I CGGCCG 1 cut(s) 948
BseXI GCAGC 5 cut(s) 54, 328, 478, 513, 634
BsgI GTGCAG 2 cut(s) 584, 773
Bsh1285I CGRYCG 1 cut(s) 951
BshFI GGCC 3 cut(s) 94, 522, 950
BshNI GGYRCC 1 cut(s) 410
BsiEI CGRYCG 1 cut(s) 951
BsiHKAI GWGCWC 2 cut(s) 452, 867
BsiHKCI CYCGRG 1 cut(s) 884
BsiSI CCGG 1 cut(s) 486
BslFI GGGAC 1 cut(s) 867
BslI CCNNNNNNNGG 3 cut(s) 41, 105, 485
BsmAI GTCTC 2 cut(s) 85, 549
BsmFI GGGAC 1 cut(s) 867
BsmI GAATGC 1 cut(s) 369
BsnI GGCC 3 cut(s) 94, 522, 950
Bso31I GGTCTC 1 cut(s) 85
BsoBI CYCGRG 1 cut(s) 884
Bsp1286I GDGCHC 2 cut(s) 452, 867
Bsp143I GATC 4 cut(s) 25, 31, 149, 442
Bsp19I CCATGG 1 cut(s) 848
BspACI CCGC 6 cut(s) 70, 194, 276, 473, 831, 947
BspANI GGCC 3 cut(s) 94, 522, 950
BspCNI CTCAG 3 cut(s) 95, 190, 433
BspHI TCATGA 1 cut(s) 319
BspLI GGNNCC 4 cut(s) 12, 412, 477, 570
BspMI ACCTGC 1 cut(s) 556
BspPI GGATC 2 cut(s) 26, 157
BspQI GCTCTTC 1 cut(s) 739
BspT107I GGYRCC 1 cut(s) 410
BspTNI GGTCTC 1 cut(s) 85
BsrFI RCCGGY 1 cut(s) 485
BsrI ACTGG 2 cut(s) 474, 722
BssAI RCCGGY 1 cut(s) 485
BssECI CCNNGG 3 cut(s) 89, 848, 999
BssMI GATC 4 cut(s) 25, 31, 149, 442
BssNI GRCGYC 1 cut(s) 411
BssT1I CCWWGG 1 cut(s) 848
Bst2UI CCWGG 1 cut(s) 90
Bst4CI ACNGT 2 cut(s) 655, 894
Bst6I CTCTTC 1 cut(s) 739
BstACI GRCGYC 1 cut(s) 411
BstBAI YACGTR 3 cut(s) 38, 50, 862
BstC8I GCNNGC 6 cut(s) 65, 524, 578, 645, 682, 996
BstDEI CTNAG 5 cut(s) 82, 177, 286, 365, 420
BstDSI CCRYGG 1 cut(s) 848
BstF5I GGATG 2 cut(s) 664, 994
BstH2I RGCGCY 2 cut(s) 414, 762
BstHHI GCGC 3 cut(s) 413, 761, 778
BstKTI GATC 4 cut(s) 28, 34, 152, 445
BstMAI GTCTC 2 cut(s) 85, 549
BstMBI GATC 4 cut(s) 25, 31, 149, 442
BstMCI CGRYCG 1 cut(s) 951
BstMWI GCNNNNNNNGC 2 cut(s) 491, 782
BstNI CCWGG 1 cut(s) 90
BstNSI RCATGY 1 cut(s) 647
BstPAI GACNNNNGTC 1 cut(s) 838
BstSCI CCNGG 1 cut(s) 88
BstV1I GCAGC 5 cut(s) 54, 328, 478, 513, 634
BstX2I RGATCY 2 cut(s) 25, 31
BstYI RGATCY 2 cut(s) 25, 31
BstZI CGGCCG 1 cut(s) 948
BsuRI GGCC 3 cut(s) 94, 522, 950
BtgI CCRYGG 1 cut(s) 848
BtsCI GGATG 2 cut(s) 664, 994
BtsIMutI CAGTG 2 cut(s) 467, 729
BveI ACCTGC 1 cut(s) 556
Cac8I GCNNGC 6 cut(s) 65, 524, 578, 645, 682, 996
CciI TCATGA 1 cut(s) 319
CfoI GCGC 3 cut(s) 413, 761, 778
Cfr10I RCCGGY 1 cut(s) 485
Cfr13I GGNCC 4 cut(s) 44, 92, 476, 833
Csp6I GTAC 1 cut(s) 965
CviAII CATG 4 cut(s) 320, 644, 808, 849
CviQI GTAC 1 cut(s) 965
DdeI CTNAG 5 cut(s) 82, 177, 286, 365, 420
DinI GGCGCC 1 cut(s) 412
DpnI GATC 4 cut(s) 27, 33, 151, 444
DpnII GATC 4 cut(s) 25, 31, 149, 442
DraI TTTAAA 1 cut(s) 427
DraIII CACNNNGTG 1 cut(s) 41
EaeI YGGCCR 1 cut(s) 948
EagI CGGCCG 1 cut(s) 948
Eam1104I CTCTTC 1 cut(s) 739
EarI CTCTTC 1 cut(s) 739
EciI GGCGGA 2 cut(s) 183, 846
EclXI CGGCCG 1 cut(s) 948
Eco130I CCWWGG 1 cut(s) 848
Eco31I GGTCTC 1 cut(s) 85
Eco47I GGWCC 3 cut(s) 44, 476, 833
Eco52I CGGCCG 1 cut(s) 948
Eco72I CACGTG 2 cut(s) 38, 50
Eco88I CYCGRG 1 cut(s) 884
EcoO109I RGGNCCY 1 cut(s) 476
EcoRII CCWGG 1 cut(s) 88
EcoT14I CCWWGG 1 cut(s) 848
EcoT22I ATGCAT 2 cut(s) 637, 645
EgeI GGCGCC 1 cut(s) 412
EheI GGCGCC 1 cut(s) 412
ErhI CCWWGG 1 cut(s) 848
FaeI CATG 4 cut(s) 323, 647, 811, 852
FalI AAGNNNNNCTT 2 cut(s) 348, 380
FaqI GGGAC 1 cut(s) 867
FatI CATG 4 cut(s) 319, 643, 807, 848
FauI CCCGC 1 cut(s) 466
FauNDI CATATG 2 cut(s) 292, 869
FblI GTMKAC 1 cut(s) 459
Fnu4HI GCNGC 6 cut(s) 68, 342, 492, 527, 648, 948
FokI GGATG 2 cut(s) 651, 1001
Fsp4HI GCNGC 6 cut(s) 68, 342, 492, 527, 648, 948
FspBI CTAG 2 cut(s) 29, 1007
GlaI GCGC 3 cut(s) 412, 760, 777
GluI GCNGC 6 cut(s) 68, 342, 492, 527, 648, 948
HaeII RGCGCY 2 cut(s) 414, 762
HaeIII GGCC 3 cut(s) 94, 522, 950
HapII CCGG 1 cut(s) 486
HhaI GCGC 3 cut(s) 413, 761, 778
Hin1I GRCGYC 1 cut(s) 411
Hin1II CATG 4 cut(s) 323, 647, 811, 852
Hin6I GCGC 3 cut(s) 411, 759, 776
HinP1I GCGC 3 cut(s) 411, 759, 776
HincII GTYRAC 3 cut(s) 235, 460, 696
HindII GTYRAC 3 cut(s) 235, 460, 696
HinfI GANTC 4 cut(s) 272, 606, 855, 880
HpaII CCGG 1 cut(s) 486
HphI GGTGA 1 cut(s) 217
Hpy166II GTNNAC 4 cut(s) 47, 235, 460, 696
Hpy188I TCNGA 4 cut(s) 85, 180, 447, 900
Hpy188III TCNNGA 4 cut(s) 29, 320, 734, 884
Hpy8I GTNNAC 4 cut(s) 47, 235, 460, 696
HpyAV CCTTC 1 cut(s) 146
HpyCH4III ACNGT 2 cut(s) 655, 894
HpyCH4IV ACGT 4 cut(s) 37, 49, 861, 923
HpyCH4V TGCA 6 cut(s) 20, 173, 565, 635, 643, 754
HpyF10VI GCNNNNNNNGC 2 cut(s) 491, 782
HpyF3I CTNAG 5 cut(s) 82, 177, 286, 365, 420
HpySE526I ACGT 4 cut(s) 37, 49, 861, 923
Hsp92I GRCGYC 1 cut(s) 411
Hsp92II CATG 4 cut(s) 323, 647, 811, 852
HspAI GCGC 3 cut(s) 411, 759, 776
KasI GGCGCC 1 cut(s) 410
Kzo9I GATC 4 cut(s) 25, 31, 149, 442
LguI GCTCTTC 1 cut(s) 739
LmnI GCTCC 2 cut(s) 447, 1003
Lsp1109I GCAGC 5 cut(s) 54, 328, 478, 513, 634
LweI GCATC 2 cut(s) 182, 644
MaeI CTAG 2 cut(s) 29, 1007
MaeII ACGT 4 cut(s) 37, 49, 861, 923
MaeIII GTNAC 3 cut(s) 840, 907, 940
MalI GATC 4 cut(s) 27, 33, 151, 444
MboI GATC 4 cut(s) 25, 31, 149, 442
MboII GAAGA 4 cut(s) 35, 370, 756, 968
MfeI CAATTG 2 cut(s) 144, 936
MflI RGATCY 2 cut(s) 25, 31
MhlI GDGCHC 2 cut(s) 452, 867
MluCI AATT 5 cut(s) 117, 144, 168, 660, 936
Mly113I GGCGCC 1 cut(s) 411
MlyI GAGTC 3 cut(s) 600, 849, 874
MmeI TCCRAC 1 cut(s) 898
MnlI CCTC 8 cut(s) 70, 193, 678, 846, 870, 907, 921, 994
Mph1103I ATGCAT 2 cut(s) 637, 645
MroXI GAANNNNTTC 1 cut(s) 212
MseI TTAA 2 cut(s) 426, 711
MslI CAYNNNNRTG 1 cut(s) 847
MspA1I CMGCKG 1 cut(s) 947
MspI CCGG 1 cut(s) 486
MspR9I CCNGG 1 cut(s) 90
MunI CAATTG 2 cut(s) 144, 936
Mva1269I GAATGC 1 cut(s) 369
MvaI CCWGG 1 cut(s) 90
MwoI GCNNNNNNNGC 2 cut(s) 491, 782
NarI GGCGCC 1 cut(s) 411
NcoI CCATGG 1 cut(s) 848
NdeI CATATG 2 cut(s) 292, 869
NdeII GATC 4 cut(s) 25, 31, 149, 442
NlaIII CATG 4 cut(s) 323, 647, 811, 852
NlaIV GGNNCC 4 cut(s) 12, 412, 477, 570
NmeAIII GCCGAG 1 cut(s) 1024
NmuCI GTSAC 1 cut(s) 840
NsiI ATGCAT 2 cut(s) 637, 645
NspI RCATGY 1 cut(s) 647
PaeI GCATGC 1 cut(s) 647
PagI TCATGA 1 cut(s) 319
PciSI GCTCTTC 1 cut(s) 739
PctI GAATGC 1 cut(s) 369
PdmI GAANNNNTTC 1 cut(s) 212
PfeI GAWTC 1 cut(s) 272
PflMI CCANNNNNTGG 2 cut(s) 41, 105
PkrI GCNGC 6 cut(s) 69, 343, 493, 528, 649, 949
PleI GAGTC 3 cut(s) 600, 849, 874
PluTI GGCGCC 1 cut(s) 414
PmaCI CACGTG 2 cut(s) 38, 50
PmlI CACGTG 2 cut(s) 38, 50
PpsI GAGTC 3 cut(s) 600, 849, 874
Ppu21I YACGTR 3 cut(s) 38, 50, 862
PpuMI RGGWCCY 1 cut(s) 476
PshAI GACNNNNGTC 1 cut(s) 838
Psp5II RGGWCCY 1 cut(s) 476
Psp6I CCWGG 1 cut(s) 88
PspCI CACGTG 2 cut(s) 38, 50
PspGI CCWGG 1 cut(s) 88
PspN4I GGNNCC 4 cut(s) 12, 412, 477, 570
PspPI GGNCC 4 cut(s) 44, 92, 476, 833
PspPPI RGGWCCY 1 cut(s) 476
PsuI RGATCY 2 cut(s) 25, 31
RsaI GTAC 1 cut(s) 966
RsaNI GTAC 1 cut(s) 965
RseI CAYNNNNRTG 1 cut(s) 847
SalI GTCGAC 1 cut(s) 458
SapI GCTCTTC 1 cut(s) 739
SaqAI TTAA 2 cut(s) 426, 711
SatI GCNGC 6 cut(s) 68, 342, 492, 527, 648, 948
Sau3AI GATC 4 cut(s) 25, 31, 149, 442
Sau96I GGNCC 4 cut(s) 44, 92, 476, 833
SchI GAGTC 3 cut(s) 600, 849, 874
ScrFI CCNGG 1 cut(s) 90
SduI GDGCHC 2 cut(s) 452, 867
SfaNI GCATC 2 cut(s) 182, 644
SfoI GGCGCC 1 cut(s) 412
SinI GGWCC 3 cut(s) 44, 476, 833
SmiMI CAYNNNNRTG 1 cut(s) 847
SphI GCATGC 1 cut(s) 647
Sse9I AATT 5 cut(s) 117, 144, 168, 660, 936
SsiI CCGC 6 cut(s) 70, 194, 276, 473, 831, 947
SspDI GGCGCC 1 cut(s) 410
SspI AATATT 1 cut(s) 391
SspMI CTAG 2 cut(s) 29, 1007
StyD4I CCNGG 1 cut(s) 88
StyI CCWWGG 1 cut(s) 848
TaaI ACNGT 2 cut(s) 655, 894
TaiI ACGT 4 cut(s) 40, 52, 864, 926
TaqI TCGA 1 cut(s) 459
TasI AATT 5 cut(s) 117, 144, 168, 660, 936
TatI WGTACW 1 cut(s) 964
TauI GCSGC 1 cut(s) 950
TfiI GAWTC 1 cut(s) 272
Tru1I TTAA 2 cut(s) 426, 711
Tru9I TTAA 2 cut(s) 426, 711
TscAI CASTG 2 cut(s) 474, 729
TseFI GTSAC 1 cut(s) 840
TseI GCWGC 5 cut(s) 67, 341, 491, 526, 647
Tsp45I GTSAC 1 cut(s) 840
TspDTI ATGAA 4 cut(s) 308, 336, 370, 653
TspRI CASTG 2 cut(s) 474, 729
Van91I CCANNNNNTGG 2 cut(s) 41, 105
VpaK11BI GGWCC 3 cut(s) 44, 476, 833
XbaI TCTAGA 1 cut(s) 28
XceI RCATGY 1 cut(s) 647
XcmI CCANNNNNNNNNTGG 3 cut(s) 102, 556, 814
XmiI GTMKAC 1 cut(s) 459
XmnI GAANNNNTTC 1 cut(s) 212
XspI CTAG 2 cut(s) 29, 1007
Zsp2I ATGCAT 2 cut(s) 637, 645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.