pycom14g03820

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0007394
Physical Location & Seq
Reverse (-)
3245464 .. 3247460
1997 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1518 bp
ATGGATTCAGAAGCATTTCCATCCTCGAAGCCGATGGTGAAATGTTGTAATTGTGAATGTAGTTGTTCGCTGGTGAGTGTCTCATCAGGGGGGACTTGGCTTCGGTCGGTAAAGAGAAAGTATGGCGAGTTTGAGGATGGTAACCGTTTCTTTATACCTGGGCTTGAACTTTATTATAATGCGCGAGTCCAGATGGAGAACGAATGCATTGCACTTCGCGAAACACTTAGTAGCCAGCAGAAAACCATACAAGATTTGTATACCGAGCTGGACGAGGAGAGGAATGCTTCATCATCCGCTGCCAATGAGGCTATGTCAATGATTTTGAGGTTGCAGAGGGAGAAGGCTGAAATACAGATGGAGGCCCGGCAATTCAAGTGTTTCGTTGAGGAGAAGATGGCACATGAGCAGCAGGAGCTTTTGGCACTGGAAGATTTGCTGTATAAGAGAGAGCAGGCGATTCAGGCACTCACATGTGAAGTGCAGGCCTATAAGCACAGAATGATGAGTTATGGGCTCACGGAGTCTGAGGCAGAAGGTGACCAGGGTTCCTTTAGCCGTATCCAAAGCGTGGGTGACTTTGAAGCCCAATATGAACTACCGACAACGCCCACATATGAATACCCACCTCTGAAATGTAAGATAAATGAGACCCAAGGTGTGTTGGAGTGTGATGATGATGCAGACATTGAGAAATATGCATTTGGTGAGAATCCTCATGGGAGAGACCATTTGAGAAATTTGGAAAGCAGGCTCTTTCAGATGGAGGGGAGTCCGAGCGGCAGTCCAATGGAGAGGGATTTTTCAGGTACCAAGAATTTTTTTGAAAAGGTGGTGGTTGGCCATTCTCCTAGGCGATCAAGACATTCTGGGAGACTTTCAAGTGACTCGCTTATGTATACAGTTAAAGAAACTGGTCCAGATTTTACTGAATCTCCTCGATATAACAACAGCTTCAGGAAGGCAGACTATGTCTCACAGTCAGAGGACTATCCAAATGCTAGAAAGTTTGATAATGTATCAGAAGTTGGAGACGACATGAGTGACAGAGTTTATACCATTGACTCTGTTCATATTGGAGCACCTTACAATGGTTATACAGAAGCCAAATCTGGAGTTACTCCTAGGGGACTTAGTGGGCCTGATTTTGAGGATCCTGATGTCATGAAGCTCTATATGAGGCTTCAGGCACTTGAGGCTGACAGGGAGTCAATGAGGCAGGCAATGCTTTCAATGCGAACAGATAAAGCTCAACTGGTATTGCTTAAAGAAATAGCTCAACATTTGTGTAATGATTTGTCACCGGAGAAACGAATGATTGTGAAGAAACCGTCTATCGTTGGACGCTTATCTTCCATGTCTGTTTTTAAGTGGATCAAGTCCTTTGTTTTCTGGAGAAGGAGAGCGTGCCGAAGCAAGCATATGTTTGGGCTATCAGCCAATGTGGGTTTGCTTATGATGTTAGACAAGGGTCCCCATGTAAGGCAGTGGAGATGTCTTACAAGCACGCAAGTGTAA

Protein Analysis

506

Amino Acids

57.74

Weight (kDa)

5.75

Isoelectric Point (pI)

50.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 71 - 161 4.1e-34 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 177
Acc65I GGTACC 1 cut(s) 809
AccB1I GGYRCC 1 cut(s) 809
AccB7I CCANNNNNTGG 1 cut(s) 571
AccBSI CCGCTC 1 cut(s) 780
AccI GTMKAC 2 cut(s) 260, 899
AccII CGCG 2 cut(s) 184, 219
AciI CCGC 2 cut(s) 297, 780
AclWI GGATC 3 cut(s) 1148, 1161, 1382
AcoI YGGCCR 1 cut(s) 841
AcsI RAATTY 2 cut(s) 739, 817
AcuI CTGAAG 2 cut(s) 940, 1169
AfaI GTAC 1 cut(s) 811
AfiI CCNNNNNNNGG 3 cut(s) 571, 1091, 1482
AflIII ACRYGT 1 cut(s) 473
AgsI TTSAA 6 cut(s) 167, 376, 584, 827, 882, 1233
AhdI GACNNNNNGTC 1 cut(s) 1207
AjnI CCWGG 2 cut(s) 157, 543
AleI CACNNNNGTG 1 cut(s) 1511
AluBI AGCT 6 cut(s) 268, 418, 954, 1171, 1250, 1277
AluI AGCT 6 cut(s) 268, 418, 954, 1171, 1250, 1277
Alw21I GWGCWC 1 cut(s) 1084
Alw26I GTCTC 6 cut(s) 85, 644, 720, 868, 979, 1026
AlwI GGATC 3 cut(s) 1148, 1161, 1382
AoxI GGCC 4 cut(s) 363, 486, 841, 1139
ApeKI GCWGC 2 cut(s) 299, 409
ApoI RAATTY 2 cut(s) 739, 817
Asp700I GAANNNNTTC 1 cut(s) 15
Asp718I GGTACC 1 cut(s) 809
AspA2I CCTAGG 2 cut(s) 851, 1124
AspLEI GCGC 1 cut(s) 184
AspS9I GGNCC 4 cut(s) 364, 917, 1139, 1472
AsuC2I CCSGG 1 cut(s) 367
AsuHPI GGTGA 6 cut(s) 49, 85, 551, 587, 719, 1293
AvaII GGWCC 2 cut(s) 917, 1472
AvrII CCTAGG 2 cut(s) 851, 1124
BalI TGGCCA 1 cut(s) 843
BamHI GGATCC 1 cut(s) 1153
BanI GGYRCC 1 cut(s) 809
BanII GRGCYC 1 cut(s) 519
Bbv12I GWGCWC 1 cut(s) 1084
BbvI GCAGC 2 cut(s) 286, 421
BccI CCATC 7 cut(s) 28, 28, 131, 187, 352, 391, 757
BceAI ACGGC 1 cut(s) 543
BcgI CGANNNNNNTGC 2 cut(s) 191, 225
BciT130I CCWGG 2 cut(s) 159, 545
BciVI GTATCC 1 cut(s) 572
BcnI CCSGG 1 cut(s) 367
BcoDI GTCTC 6 cut(s) 85, 644, 720, 868, 979, 1026
BfaI CTAG 3 cut(s) 852, 1002, 1125
BfuI GTATCC 1 cut(s) 572
BglI GCCNNNNNGGC 1 cut(s) 308
BisI GCNGC 3 cut(s) 300, 410, 781
BlnI CCTAGG 2 cut(s) 851, 1124
BlsI GCNGC 3 cut(s) 301, 411, 782
Bme1390I CCNGG 3 cut(s) 159, 367, 545
Bme18I GGWCC 2 cut(s) 917, 1472
BmeRI GACNNNNNGTC 1 cut(s) 1207
BmgT120I GGNCC 4 cut(s) 364, 917, 1139, 1472
BmiI GGNNCC 5 cut(s) 550, 811, 1155, 1473, 1474
BmrFI CCNGG 3 cut(s) 159, 367, 545
BmsI GCATC 1 cut(s) 670
BoxI GACNNNNGTC 1 cut(s) 1470
BpmI CTGGAG 2 cut(s) 1134, 1414
BpuEI CTTGAG 1 cut(s) 1214
BpuMI CCSGG 1 cut(s) 367
BsaI GGTCTC 2 cut(s) 644, 720
BsaJI CCNNGG 5 cut(s) 158, 544, 655, 851, 1124
BsaWI WCCGGW 1 cut(s) 1303
BsaXI ACNNNNNCTCC 2 cut(s) 919, 949
Bsc4I CCNNNNNNNGG 3 cut(s) 571, 1091, 1482
Bse1I ACTGG 3 cut(s) 432, 919, 1260
Bse3DI GCAATG 2 cut(s) 207, 1230
BseBI CCWGG 2 cut(s) 159, 545
BseDI CCNNGG 5 cut(s) 158, 544, 655, 851, 1124
BseGI GGATG 3 cut(s) 20, 142, 293
BseLI CCNNNNNNNGG 3 cut(s) 571, 1091, 1482
BseMI GCAATG 2 cut(s) 207, 1230
BseMII CTCAG 1 cut(s) 519
BseNI ACTGG 3 cut(s) 432, 919, 1260
BseRI GAGGAG 3 cut(s) 290, 404, 927
BseXI GCAGC 2 cut(s) 286, 421
BsgI GTGCAG 1 cut(s) 503
Bsh1236I CGCG 2 cut(s) 184, 219
Bsh1285I CGRYCG 1 cut(s) 107
BshFI GGCC 4 cut(s) 365, 488, 843, 1141
BshNI GGYRCC 1 cut(s) 809
BsiEI CGRYCG 1 cut(s) 107
BsiHKAI GWGCWC 1 cut(s) 1084
BsiSI CCGG 2 cut(s) 367, 1304
BslFI GGGAC 3 cut(s) 106, 1143, 1458
BslI CCNNNNNNNGG 3 cut(s) 571, 1091, 1482
BsmAI GTCTC 6 cut(s) 85, 644, 720, 868, 979, 1026
BsmBI CGTCTC 1 cut(s) 1026
BsmFI GGGAC 3 cut(s) 106, 1143, 1458
BsmI GAATGC 2 cut(s) 209, 289
BsnI GGCC 4 cut(s) 365, 488, 843, 1141
Bso31I GGTCTC 2 cut(s) 644, 720
Bsp1286I GDGCHC 2 cut(s) 519, 1084
Bsp143I GATC 3 cut(s) 857, 1153, 1374
Bsp68I TCGCGA 1 cut(s) 219
BspACI CCGC 2 cut(s) 297, 780
BspANI GGCC 4 cut(s) 365, 488, 843, 1141
BspCNI CTCAG 1 cut(s) 520
BspFNI CGCG 2 cut(s) 184, 219
BspHI TCATGA 1 cut(s) 1164
BspLI GGNNCC 5 cut(s) 550, 811, 1155, 1473, 1474
BspPI GGATC 3 cut(s) 1148, 1161, 1382
BspT107I GGYRCC 1 cut(s) 809
BspTNI GGTCTC 2 cut(s) 644, 720
BsrBI CCGCTC 1 cut(s) 780
BsrDI GCAATG 2 cut(s) 207, 1230
BsrI ACTGG 3 cut(s) 432, 919, 1260
BssECI CCNNGG 5 cut(s) 158, 544, 655, 851, 1124
BssMI GATC 3 cut(s) 857, 1153, 1374
BssNAI GTATAC 2 cut(s) 261, 900
BssT1I CCWWGG 3 cut(s) 655, 851, 1124
Bst1107I GTATAC 2 cut(s) 261, 900
Bst2UI CCWGG 2 cut(s) 159, 545
Bst4CI ACNGT 4 cut(s) 146, 904, 981, 1332
BstAPI GCANNNNNTGC 1 cut(s) 1225
BstC8I GCNNGC 8 cut(s) 236, 456, 486, 752, 1221, 1408, 1418, 1508
BstDEI CTNAG 3 cut(s) 227, 528, 1133
BstEII GGTNACC 2 cut(s) 140, 539
BstF5I GGATG 3 cut(s) 20, 142, 293
BstFNI CGCG 2 cut(s) 184, 219
BstHHI GCGC 1 cut(s) 184
BstKTI GATC 3 cut(s) 860, 1156, 1377
BstMAI GTCTC 6 cut(s) 85, 644, 720, 868, 979, 1026
BstMBI GATC 3 cut(s) 857, 1153, 1374
BstMCI CGRYCG 1 cut(s) 107
BstMWI GCNNNNNNNGC 6 cut(s) 308, 415, 464, 1196, 1225, 1234
BstNI CCWGG 2 cut(s) 159, 545
BstNSI RCATGY 1 cut(s) 477
BstPAI GACNNNNGTC 1 cut(s) 1470
BstPI GGTNACC 2 cut(s) 140, 539
BstSCI CCNGG 3 cut(s) 157, 365, 543
BstUI CGCG 2 cut(s) 184, 219
BstV1I GCAGC 2 cut(s) 286, 421
BstX2I RGATCY 1 cut(s) 1153
BstYI RGATCY 1 cut(s) 1153
BstZ17I GTATAC 2 cut(s) 261, 900
BsuI GTATCC 1 cut(s) 572
BsuRI GGCC 4 cut(s) 365, 488, 843, 1141
BtsCI GGATG 3 cut(s) 20, 142, 293
BtsI GCAGTG 1 cut(s) 1493
BtsIMutI CAGTG 2 cut(s) 425, 1493
BtuMI TCGCGA 1 cut(s) 219
Cac8I GCNNGC 8 cut(s) 236, 456, 486, 752, 1221, 1408, 1418, 1508
CciI TCATGA 1 cut(s) 1164
CfoI GCGC 1 cut(s) 184
Cfr13I GGNCC 4 cut(s) 364, 917, 1139, 1472
CseI GACGC 1 cut(s) 1353
Csp6I GTAC 1 cut(s) 810
CviAII CATG 7 cut(s) 404, 474, 719, 1039, 1165, 1357, 1478
CviQI GTAC 1 cut(s) 810
DdeI CTNAG 3 cut(s) 227, 528, 1133
DpnI GATC 3 cut(s) 859, 1155, 1376
DpnII GATC 3 cut(s) 857, 1153, 1374
DriI GACNNNNNGTC 1 cut(s) 1207
EaeI YGGCCR 1 cut(s) 841
Eam1105I GACNNNNNGTC 1 cut(s) 1207
Eco130I CCWWGG 3 cut(s) 655, 851, 1124
Eco147I AGGCCT 1 cut(s) 488
Eco24I GRGCYC 1 cut(s) 519
Eco31I GGTCTC 2 cut(s) 644, 720
Eco47I GGWCC 2 cut(s) 917, 1472
Eco57I CTGAAG 2 cut(s) 940, 1169
Eco91I GGTNACC 2 cut(s) 140, 539
EcoO109I RGGNCCY 1 cut(s) 1472
EcoO65I GGTNACC 2 cut(s) 140, 539
EcoRII CCWGG 2 cut(s) 157, 543
EcoT14I CCWWGG 3 cut(s) 655, 851, 1124
EcoT22I ATGCAT 2 cut(s) 209, 703
EcoT38I GRGCYC 1 cut(s) 519
ErhI CCWWGG 3 cut(s) 655, 851, 1124
Esp3I CGTCTC 1 cut(s) 1026
FaeI CATG 7 cut(s) 407, 477, 722, 1042, 1168, 1360, 1481
FaqI GGGAC 3 cut(s) 106, 1143, 1458
FatI CATG 7 cut(s) 403, 473, 718, 1038, 1164, 1356, 1477
FauNDI CATATG 2 cut(s) 616, 1422
FblI GTMKAC 2 cut(s) 260, 899
Fnu4HI GCNGC 3 cut(s) 300, 410, 781
FokI GGATG 3 cut(s) 7, 149, 280
FriOI GRGCYC 1 cut(s) 519
Fsp4HI GCNGC 3 cut(s) 300, 410, 781
FspBI CTAG 3 cut(s) 852, 1002, 1125
GlaI GCGC 1 cut(s) 183
GluI GCNGC 3 cut(s) 300, 410, 781
GsuI CTGGAG 2 cut(s) 1134, 1414
HaeIII GGCC 4 cut(s) 365, 488, 843, 1141
HapII CCGG 2 cut(s) 367, 1304
HgaI GACGC 1 cut(s) 1353
HhaI GCGC 1 cut(s) 184
Hin1II CATG 7 cut(s) 407, 477, 722, 1042, 1168, 1360, 1481
Hin6I GCGC 1 cut(s) 182
HinP1I GCGC 1 cut(s) 182
HpaII CCGG 2 cut(s) 367, 1304
HphI GGTGA 6 cut(s) 49, 85, 551, 587, 719, 1293
Hpy166II GTNNAC 2 cut(s) 261, 900
Hpy188I TCNGA 7 cut(s) 10, 529, 633, 762, 777, 985, 1024
Hpy188III TCNNGA 9 cut(s) 190, 218, 861, 920, 958, 1113, 1157, 1165, 1393
Hpy8I GTNNAC 2 cut(s) 261, 900
HpyAV CCTTC 4 cut(s) 337, 530, 955, 1392
HpyCH4III ACNGT 4 cut(s) 146, 904, 981, 1332
HpyCH4V TGCA 6 cut(s) 207, 212, 334, 484, 683, 701
HpyF10VI GCNNNNNNNGC 6 cut(s) 308, 415, 464, 1196, 1225, 1234
HpyF3I CTNAG 3 cut(s) 227, 528, 1133
Hsp92II CATG 7 cut(s) 407, 477, 722, 1042, 1168, 1360, 1481
HspAI GCGC 1 cut(s) 182
KflI GGGWCCC 1 cut(s) 1472
KpnI GGTACC 1 cut(s) 813
Kzo9I GATC 3 cut(s) 857, 1153, 1374
LmnI GCTCC 2 cut(s) 415, 1079
Lsp1109I GCAGC 2 cut(s) 286, 421
LweI GCATC 1 cut(s) 670
MaeI CTAG 3 cut(s) 852, 1002, 1125
MaeIII GTNAC 7 cut(s) 140, 539, 575, 884, 1043, 1117, 1299
MalI GATC 3 cut(s) 859, 1155, 1376
MbiI CCGCTC 1 cut(s) 780
MboI GATC 3 cut(s) 857, 1153, 1374
MboII GAAGA 4 cut(s) 406, 443, 1336, 1344
MflI RGATCY 1 cut(s) 1153
MhlI GDGCHC 2 cut(s) 519, 1084
MlsI TGGCCA 1 cut(s) 843
MluCI AATT 4 cut(s) 49, 371, 739, 817
MluNI TGGCCA 1 cut(s) 843
MlyI GAGTC 6 cut(s) 195, 533, 781, 881, 1058, 1217
MmeI TCCRAC 3 cut(s) 645, 1009, 1321
Mox20I TGGCCA 1 cut(s) 843
Mph1103I ATGCAT 2 cut(s) 209, 703
MroXI GAANNNNTTC 1 cut(s) 15
MscI TGGCCA 1 cut(s) 843
MseI TTAA 3 cut(s) 906, 1266, 1368
MslI CAYNNNNRTG 2 cut(s) 472, 1511
Msp20I TGGCCA 1 cut(s) 843
MspA1I CMGCKG 1 cut(s) 299
MspI CCGG 2 cut(s) 367, 1304
MspR9I CCNGG 3 cut(s) 159, 367, 545
Mva1269I GAATGC 2 cut(s) 209, 289
MvaI CCWGG 2 cut(s) 159, 545
MvnI CGCG 2 cut(s) 184, 219
MwoI GCNNNNNNNGC 6 cut(s) 308, 415, 464, 1196, 1225, 1234
NciI CCSGG 1 cut(s) 367
NdeI CATATG 2 cut(s) 616, 1422
NdeII GATC 3 cut(s) 857, 1153, 1374
NlaIII CATG 7 cut(s) 407, 477, 722, 1042, 1168, 1360, 1481
NlaIV GGNNCC 5 cut(s) 550, 811, 1155, 1473, 1474
NmuCI GTSAC 5 cut(s) 539, 575, 884, 1043, 1299
NruI TCGCGA 1 cut(s) 219
NsiI ATGCAT 2 cut(s) 209, 703
NspI RCATGY 1 cut(s) 477
OliI CACNNNNGTG 1 cut(s) 1511
PagI TCATGA 1 cut(s) 1164
PceI AGGCCT 1 cut(s) 488
PciI ACATGT 1 cut(s) 473
PctI GAATGC 2 cut(s) 209, 289
PdmI GAANNNNTTC 1 cut(s) 15
PfeI GAWTC 4 cut(s) 5, 460, 712, 932
PflFI GACNNNGTC 1 cut(s) 971
PflMI CCANNNNNTGG 1 cut(s) 571
PkrI GCNGC 3 cut(s) 301, 411, 782
PleI GAGTC 6 cut(s) 194, 532, 780, 881, 1058, 1216
PpsI GAGTC 6 cut(s) 194, 532, 780, 881, 1058, 1216
PpuMI RGGWCCY 1 cut(s) 1472
PscI ACATGT 1 cut(s) 473
PshAI GACNNNNGTC 1 cut(s) 1470
PsiI TTATAA 1 cut(s) 177
Psp5II RGGWCCY 1 cut(s) 1472
Psp6I CCWGG 2 cut(s) 157, 543
PspEI GGTNACC 2 cut(s) 140, 539
PspGI CCWGG 2 cut(s) 157, 543
PspN4I GGNNCC 5 cut(s) 550, 811, 1155, 1473, 1474
PspPI GGNCC 4 cut(s) 364, 917, 1139, 1472
PspPPI RGGWCCY 1 cut(s) 1472
PsuI RGATCY 1 cut(s) 1153
PsyI GACNNNGTC 1 cut(s) 971
RruI TCGCGA 1 cut(s) 219
RsaI GTAC 1 cut(s) 811
RsaNI GTAC 1 cut(s) 810
RseI CAYNNNNRTG 2 cut(s) 472, 1511
SaqAI TTAA 3 cut(s) 906, 1266, 1368
SatI GCNGC 3 cut(s) 300, 410, 781
Sau3AI GATC 3 cut(s) 857, 1153, 1374
Sau96I GGNCC 4 cut(s) 364, 917, 1139, 1472
SchI GAGTC 6 cut(s) 195, 533, 781, 881, 1058, 1217
ScrFI CCNGG 3 cut(s) 159, 367, 545
SduI GDGCHC 2 cut(s) 519, 1084
SfaNI GCATC 1 cut(s) 670
SinI GGWCC 2 cut(s) 917, 1472
SmiMI CAYNNNNRTG 2 cut(s) 472, 1511
SmlI CTYRAG 1 cut(s) 1193
SmoI CTYRAG 1 cut(s) 1193
Sse9I AATT 4 cut(s) 49, 371, 739, 817
SseBI AGGCCT 1 cut(s) 488
SsiI CCGC 2 cut(s) 297, 780
SspMI CTAG 3 cut(s) 852, 1002, 1125
StuI AGGCCT 1 cut(s) 488
StyD4I CCNGG 3 cut(s) 157, 365, 543
StyI CCWWGG 3 cut(s) 655, 851, 1124
TaaI ACNGT 4 cut(s) 146, 904, 981, 1332
TaqI TCGA 2 cut(s) 26, 940
TaqII GACCGA 1 cut(s) 93
TasI AATT 4 cut(s) 49, 371, 739, 817
TauI GCSGC 1 cut(s) 783
TfiI GAWTC 4 cut(s) 5, 460, 712, 932
Tru1I TTAA 3 cut(s) 906, 1266, 1368
Tru9I TTAA 3 cut(s) 906, 1266, 1368
TscAI CASTG 2 cut(s) 432, 1493
TseFI GTSAC 5 cut(s) 539, 575, 884, 1043, 1299
TseI GCWGC 2 cut(s) 299, 409
Tsp45I GTSAC 5 cut(s) 539, 575, 884, 1043, 1299
TspDTI ATGAA 5 cut(s) 279, 609, 633, 1061, 1181
TspGWI ACGGA 1 cut(s) 536
TspRI CASTG 2 cut(s) 432, 1493
Tth111I GACNNNGTC 1 cut(s) 971
Van91I CCANNNNNTGG 1 cut(s) 571
VpaK11BI GGWCC 2 cut(s) 917, 1472
XapI RAATTY 2 cut(s) 739, 817
XceI RCATGY 1 cut(s) 477
XmaJI CCTAGG 2 cut(s) 851, 1124
XmiI GTMKAC 2 cut(s) 260, 899
XmnI GAANNNNTTC 1 cut(s) 15
XspI CTAG 3 cut(s) 852, 1002, 1125
Zsp2I ATGCAT 2 cut(s) 209, 703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.