pycom14g06300

Transcription initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0014000
Physical Location & Seq
Reverse (-)
6154854 .. 6159347
4494 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 939 bp
ATGTCCGATGCGTATTGCTCCGACTGCAAGCGCCAGACGGAGGTCGTGAGAGACCACTCGGCGGGTGATACCGTCTGCTCCGAGTGCGGTCTAGTGCTCGAGTCTCATTCCATTGATGAGACCTCGGAGTGGCGTACCTTCGCGAACGAGTCGGGCGATAACGACCCGGTCCGTGTTGGTGGGCCTACCAATCCCCTGTTGGCCGATGGCGGGCTGTCCACCGTCATAGCCAAGCCCAATGGGGCTTCTGGTGAGTTCTTGTCTTCGTCCTTGGGTCGGTGGCAGAACCGTGGGTCTAATCCGGATCGGGGCTTGATAATGGCGTTCAAAACCATCGCTACCATGTCCGATAGGTTGGGCCTTGTTGCAACCATCAAGGATCGGGCTAATGAGATATATAAGAGGGTGGAGGATCAGAAATCTAGTAGGGGAAGAAATCAGGATGCACTATTGGCTGCTTGCCTGTACATTGCTTGTCGACAAGAAGACAAGCCACGCACTGTAAAGGAAATTTGCTCTGTCGCCAATGGAGCCACAAAGAAGGAAATTGGCCGAGCAAAAGAATACATAGTGAAACAGCTGGGATTAGAGAATGGTCAGTCGGTGGAGATGGGAACAATACATGCTGGGGACTTTATGAGGCGATTTTGTTCCAATCTGGGTATGATTAATCAAGCAGTCAAGGCTGCTCATGAAGCTGTGACAAAGTCCGAAGAATTTGATATAAGGCGGAGCCCTATATCGATAGCAGCGGCAGTTATTTATATTATCACCCAGCTTTCAGACGATAAGAAGCCTCTCAAAGATATCTCCGTTGCCACCGGAGTAGCGGAAGGAACAATCAGAAACTCGTACAAAGACCTTTATCCCCATGTGTCAAAGATTATACCGGGCTGGTACGCAACAGAGGATGCTATCAAGAGCCTTTGCAGCCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIB TF_Zn_Ribbon

Protein Analysis

313

Amino Acids

33.98

Weight (kDa)

6.54

Isoelectric Point (pI)

38.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_Ribbon_TF PF08271 5 - 47 2.3e-15 TFIIB zinc-binding
TFIIB PF00382 103 - 185 5.3e-21 Transcription factor TFIIB repeat
TFIIB PF00382 204 - 290 1.2e-12 Transcription factor TFIIB repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014000)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10330
fragaria_vesca FvH4_6g24330
malus_domestica MD12G1075300.v1.1 MD14G1073800.v1.1
prunus_persica Prupe.7G055400_v2.0.a1
pyrus_communis pycom12g06480 pycom14g06300
rosa_chinensis RchiOBHm_Chr3g0483261
rosa_laevigata RLG00000023294
rosa_multiflora Rmu_sc0007550.1_g000009
rosa_roxburghii Rroxscaffold_6G00398520
rosa_rugosa Rorug03G0205000
rosa_samantha Rh3AG253400 Rh3BG290500 Rh3CG287300 Rh3DG283100
rosa_wichuraiana Rw3G022920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 478
AccII CGCG 1 cut(s) 143
AccIII TCCGGA 1 cut(s) 301
AciI CCGC 6 cut(s) 62, 87, 210, 730, 752, 830
AclWI GGATC 3 cut(s) 312, 387, 420
AcoI YGGCCR 2 cut(s) 201, 550
AcsI RAATTY 2 cut(s) 510, 716
AfaI GTAC 4 cut(s) 136, 467, 854, 899
AfiI CCNNNNNNNGG 6 cut(s) 40, 61, 129, 210, 276, 308
AgsI TTSAA 1 cut(s) 328
AluBI AGCT 3 cut(s) 580, 698, 778
AluI AGCT 3 cut(s) 580, 698, 778
Alw21I GWGCWC 1 cut(s) 99
Alw26I GTCTC 3 cut(s) 45, 108, 113
AlwI GGATC 3 cut(s) 312, 387, 420
Ama87I CYCGRG 1 cut(s) 98
Aor13HI TCCGGA 1 cut(s) 301
AoxI GGCC 4 cut(s) 182, 201, 358, 550
ApeKI GCWGC 4 cut(s) 455, 686, 749, 930
ApoI RAATTY 2 cut(s) 510, 716
AseI ATTAAT 1 cut(s) 669
AspLEI GCGC 1 cut(s) 33
AspS9I GGNCC 3 cut(s) 169, 182, 358
AsuC2I CCSGG 2 cut(s) 167, 891
AsuHPI GGTGA 3 cut(s) 77, 263, 763
AvaI CYCGRG 1 cut(s) 98
AvaII GGWCC 1 cut(s) 169
BanII GRGCYC 1 cut(s) 737
BbsI GAAGAC 2 cut(s) 255, 492
Bbv12I GWGCWC 1 cut(s) 99
BbvI GCAGC 3 cut(s) 442, 673, 761
BccI CCATC 4 cut(s) 200, 341, 380, 604
BcnI CCSGG 2 cut(s) 167, 891
BcoDI GTCTC 3 cut(s) 45, 108, 113
BfaI CTAG 2 cut(s) 92, 423
BfoI RGCGCY 1 cut(s) 34
BisI GCNGC 5 cut(s) 456, 687, 750, 753, 931
BlsI GCNGC 5 cut(s) 457, 688, 751, 754, 932
Bme1390I CCNGG 2 cut(s) 167, 891
Bme18I GGWCC 1 cut(s) 169
BmeT110I CYCGRG 1 cut(s) 98
BmgT120I GGNCC 3 cut(s) 169, 182, 358
BmiI GGNNCC 2 cut(s) 532, 734
BmrFI CCNGG 2 cut(s) 167, 891
BmsI GCATC 2 cut(s) 433, 901
BoxI GACNNNNGTC 1 cut(s) 41
BpiI GAAGAC 2 cut(s) 255, 492
BpuMI CCSGG 2 cut(s) 167, 891
Bsa29I ATCGAT 1 cut(s) 743
BsaI GGTCTC 2 cut(s) 45, 113
BsaJI CCNNGG 3 cut(s) 123, 270, 289
BsaWI WCCGGW 2 cut(s) 301, 821
Bsc4I CCNNNNNNNGG 6 cut(s) 40, 61, 129, 210, 276, 308
Bse3DI GCAATG 1 cut(s) 468
BseAI TCCGGA 1 cut(s) 301
BseCI ATCGAT 1 cut(s) 743
BseDI CCNNGG 3 cut(s) 123, 270, 289
BseGI GGATG 2 cut(s) 448, 916
BseLI CCNNNNNNNGG 6 cut(s) 40, 61, 129, 210, 276, 308
BseMI GCAATG 1 cut(s) 468
BseXI GCAGC 3 cut(s) 442, 673, 761
BseYI CCCAGC 3 cut(s) 580, 626, 774
Bsh1236I CGCG 1 cut(s) 143
BshFI GGCC 4 cut(s) 184, 203, 360, 552
BshVI ATCGAT 1 cut(s) 743
BsiHKAI GWGCWC 1 cut(s) 99
BsiHKCI CYCGRG 1 cut(s) 98
BsiSI CCGG 4 cut(s) 167, 302, 822, 890
BslFI GGGAC 1 cut(s) 644
BslI CCNNNNNNNGG 6 cut(s) 40, 61, 129, 210, 276, 308
BsmAI GTCTC 3 cut(s) 45, 108, 113
BsmFI GGGAC 1 cut(s) 644
BsnI GGCC 4 cut(s) 184, 203, 360, 552
Bso31I GGTCTC 2 cut(s) 45, 113
BsoBI CYCGRG 1 cut(s) 98
Bsp1286I GDGCHC 2 cut(s) 99, 737
Bsp13I TCCGGA 1 cut(s) 301
Bsp1407I TGTACA 1 cut(s) 465
Bsp143I GATC 3 cut(s) 304, 379, 412
Bsp68I TCGCGA 1 cut(s) 143
BspACI CCGC 6 cut(s) 62, 87, 210, 730, 752, 830
BspANI GGCC 4 cut(s) 184, 203, 360, 552
BspDI ATCGAT 1 cut(s) 743
BspEI TCCGGA 1 cut(s) 301
BspFNI CGCG 1 cut(s) 143
BspHI TCATGA 1 cut(s) 691
BspLI GGNNCC 2 cut(s) 532, 734
BspPI GGATC 3 cut(s) 312, 387, 420
BspTNI GGTCTC 2 cut(s) 45, 113
BsrDI GCAATG 1 cut(s) 468
BsrGI TGTACA 1 cut(s) 465
BssECI CCNNGG 3 cut(s) 123, 270, 289
BssMI GATC 3 cut(s) 304, 379, 412
BssT1I CCWWGG 1 cut(s) 270
Bst4CI ACNGT 4 cut(s) 73, 223, 290, 502
BstAUI TGTACA 1 cut(s) 465
BstC8I GCNNGC 3 cut(s) 29, 212, 460
BstDSI CCRYGG 1 cut(s) 289
BstF5I GGATG 2 cut(s) 448, 916
BstFNI CGCG 1 cut(s) 143
BstH2I RGCGCY 1 cut(s) 34
BstHHI GCGC 1 cut(s) 33
BstKTI GATC 3 cut(s) 307, 382, 415
BstMAI GTCTC 3 cut(s) 45, 108, 113
BstMBI GATC 3 cut(s) 304, 379, 412
BstMWI GCNNNNNNNGC 7 cut(s) 24, 84, 452, 530, 683, 695, 930
BstNSI RCATGY 1 cut(s) 626
BstPAI GACNNNNGTC 1 cut(s) 41
BstSCI CCNGG 2 cut(s) 165, 889
BstUI CGCG 1 cut(s) 143
BstV1I GCAGC 3 cut(s) 442, 673, 761
BstV2I GAAGAC 2 cut(s) 255, 492
Bsu15I ATCGAT 1 cut(s) 743
BsuRI GGCC 4 cut(s) 184, 203, 360, 552
BsuTUI ATCGAT 1 cut(s) 743
BtgI CCRYGG 1 cut(s) 289
BtgZI GCGATG 1 cut(s) 319
BtsCI GGATG 2 cut(s) 448, 916
BtsIMutI CAGTG 1 cut(s) 498
BtuMI TCGCGA 1 cut(s) 143
Cac8I GCNNGC 3 cut(s) 29, 212, 460
CciI TCATGA 1 cut(s) 691
CfoI GCGC 1 cut(s) 33
Cfr13I GGNCC 3 cut(s) 169, 182, 358
ClaI ATCGAT 1 cut(s) 743
CpoI CGGWCCG 1 cut(s) 169
Csp6I GTAC 4 cut(s) 135, 466, 853, 898
CspI CGGWCCG 1 cut(s) 169
CviAII CATG 4 cut(s) 343, 623, 692, 872
CviQI GTAC 4 cut(s) 135, 466, 853, 898
DpnI GATC 3 cut(s) 306, 381, 414
DpnII GATC 3 cut(s) 304, 379, 412
EaeI YGGCCR 2 cut(s) 201, 550
EciI GGCGGA 1 cut(s) 745
Eco130I CCWWGG 1 cut(s) 270
Eco24I GRGCYC 1 cut(s) 737
Eco31I GGTCTC 2 cut(s) 45, 113
Eco32I GATATC 1 cut(s) 808
Eco47I GGWCC 1 cut(s) 169
Eco88I CYCGRG 1 cut(s) 98
EcoRV GATATC 1 cut(s) 808
EcoT14I CCWWGG 1 cut(s) 270
EcoT38I GRGCYC 1 cut(s) 737
ErhI CCWWGG 1 cut(s) 270
FaeI CATG 4 cut(s) 346, 626, 695, 875
FaqI GGGAC 1 cut(s) 644
FatI CATG 4 cut(s) 342, 622, 691, 871
FauI CCCGC 2 cut(s) 55, 203
FblI GTMKAC 1 cut(s) 478
Fnu4HI GCNGC 5 cut(s) 456, 687, 750, 753, 931
FokI GGATG 2 cut(s) 455, 923
FriOI GRGCYC 1 cut(s) 737
Fsp4HI GCNGC 5 cut(s) 456, 687, 750, 753, 931
FspBI CTAG 2 cut(s) 92, 423
GlaI GCGC 1 cut(s) 32
GluI GCNGC 5 cut(s) 456, 687, 750, 753, 931
GsaI CCCAGC 3 cut(s) 584, 630, 778
HaeII RGCGCY 1 cut(s) 34
HaeIII GGCC 4 cut(s) 184, 203, 360, 552
HapII CCGG 4 cut(s) 167, 302, 822, 890
HhaI GCGC 1 cut(s) 33
Hin1II CATG 4 cut(s) 346, 626, 695, 875
Hin6I GCGC 1 cut(s) 31
HinP1I GCGC 1 cut(s) 31
HincII GTYRAC 1 cut(s) 479
HindII GTYRAC 1 cut(s) 479
HinfI GANTC 2 cut(s) 101, 149
HpaII CCGG 4 cut(s) 167, 302, 822, 890
HphI GGTGA 3 cut(s) 77, 263, 763
Hpy166II GTNNAC 2 cut(s) 219, 479
Hpy188I TCNGA 9 cut(s) 7, 22, 82, 127, 349, 417, 712, 784, 845
Hpy188III TCNNGA 6 cut(s) 46, 142, 302, 440, 692, 919
Hpy8I GTNNAC 2 cut(s) 219, 479
HpyAV CCTTC 3 cut(s) 148, 535, 827
HpyCH4III ACNGT 4 cut(s) 73, 223, 290, 502
HpyCH4V TGCA 4 cut(s) 27, 368, 446, 930
HpyF10VI GCNNNNNNNGC 7 cut(s) 24, 84, 452, 530, 683, 695, 930
Hsp92II CATG 4 cut(s) 346, 626, 695, 875
HspAI GCGC 1 cut(s) 31
Kpn2I TCCGGA 1 cut(s) 301
Kzo9I GATC 3 cut(s) 304, 379, 412
LmnI GCTCC 4 cut(s) 23, 83, 530, 732
Lsp1109I GCAGC 3 cut(s) 442, 673, 761
LweI GCATC 2 cut(s) 433, 901
MaeI CTAG 2 cut(s) 92, 423
MaeIII GTNAC 1 cut(s) 700
MalI GATC 3 cut(s) 306, 381, 414
MboI GATC 3 cut(s) 304, 379, 412
MboII GAAGA 4 cut(s) 255, 444, 497, 725
MhlI GDGCHC 2 cut(s) 99, 737
MluCI AATT 3 cut(s) 510, 546, 716
MlyI GAGTC 2 cut(s) 110, 158
MmeI TCCRAC 1 cut(s) 45
MnlI CCTC 7 cut(s) 34, 133, 396, 403, 633, 807, 901
MroI TCCGGA 1 cut(s) 301
MseI TTAA 1 cut(s) 669
MspA1I CMGCKG 2 cut(s) 580, 752
MspI CCGG 4 cut(s) 167, 302, 822, 890
MspR9I CCNGG 2 cut(s) 167, 891
MvnI CGCG 1 cut(s) 143
MwoI GCNNNNNNNGC 7 cut(s) 24, 84, 452, 530, 683, 695, 930
NciI CCSGG 2 cut(s) 167, 891
NdeII GATC 3 cut(s) 304, 379, 412
NlaIII CATG 4 cut(s) 346, 626, 695, 875
NlaIV GGNNCC 2 cut(s) 532, 734
NmeAIII GCCGAG 2 cut(s) 38, 578
NmuCI GTSAC 1 cut(s) 700
NruI TCGCGA 1 cut(s) 143
NspI RCATGY 1 cut(s) 626
PaeR7I CTCGAG 1 cut(s) 98
PagI TCATGA 1 cut(s) 691
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PflFI GACNNNGTC 2 cut(s) 167, 706
PkrI GCNGC 5 cut(s) 457, 688, 751, 754, 932
PleI GAGTC 2 cut(s) 109, 157
PpsI GAGTC 2 cut(s) 109, 157
PshAI GACNNNNGTC 1 cut(s) 41
PshBI ATTAAT 1 cut(s) 669
PspFI CCCAGC 3 cut(s) 580, 626, 774
PspN4I GGNNCC 2 cut(s) 532, 734
PspPI GGNCC 3 cut(s) 169, 182, 358
PspXI VCTCGAGB 1 cut(s) 98
PsyI GACNNNGTC 2 cut(s) 167, 706
PvuII CAGCTG 1 cut(s) 580
RruI TCGCGA 1 cut(s) 143
RsaI GTAC 4 cut(s) 136, 467, 854, 899
RsaNI GTAC 4 cut(s) 135, 466, 853, 898
Rsr2I CGGWCCG 1 cut(s) 169
RsrII CGGWCCG 1 cut(s) 169
SalI GTCGAC 1 cut(s) 477
SaqAI TTAA 1 cut(s) 669
SatI GCNGC 5 cut(s) 456, 687, 750, 753, 931
Sau3AI GATC 3 cut(s) 304, 379, 412
Sau96I GGNCC 3 cut(s) 169, 182, 358
SchI GAGTC 2 cut(s) 110, 158
ScrFI CCNGG 2 cut(s) 167, 891
SduI GDGCHC 2 cut(s) 99, 737
SetI ASST 8 cut(s) 45, 125, 140, 356, 582, 700, 780, 864
SfaNI GCATC 2 cut(s) 433, 901
Sfr274I CTCGAG 1 cut(s) 98
SinI GGWCC 1 cut(s) 169
SlaI CTCGAG 1 cut(s) 98
SmlI CTYRAG 1 cut(s) 98
SmoI CTYRAG 1 cut(s) 98
Sse9I AATT 3 cut(s) 510, 546, 716
SsiI CCGC 6 cut(s) 62, 87, 210, 730, 752, 830
SspMI CTAG 2 cut(s) 92, 423
StyD4I CCNGG 2 cut(s) 165, 889
StyI CCWWGG 1 cut(s) 270
TaaI ACNGT 4 cut(s) 73, 223, 290, 502
TaqI TCGA 3 cut(s) 99, 478, 743
TasI AATT 3 cut(s) 510, 546, 716
TatI WGTACW 1 cut(s) 465
TauI GCSGC 1 cut(s) 755
Tru1I TTAA 1 cut(s) 669
Tru9I TTAA 1 cut(s) 669
TscAI CASTG 1 cut(s) 505
TseFI GTSAC 1 cut(s) 700
TseI GCWGC 4 cut(s) 455, 686, 749, 930
Tsp45I GTSAC 1 cut(s) 700
TspDTI ATGAA 1 cut(s) 708
TspGWI ACGGA 3 cut(s) 53, 161, 802
TspRI CASTG 1 cut(s) 505
Tth111I GACNNNGTC 2 cut(s) 167, 706
VpaK11BI GGWCC 1 cut(s) 169
VspI ATTAAT 1 cut(s) 669
XapI RAATTY 2 cut(s) 510, 716
XceI RCATGY 1 cut(s) 626
XcmI CCANNNNNNNNNTGG 1 cut(s) 196
XhoI CTCGAG 1 cut(s) 98
XmiI GTMKAC 1 cut(s) 478
XspI CTAG 2 cut(s) 92, 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.