pycom14g06330

Protein of unknown function (DUF563)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0018886
Physical Location & Seq
Forward (+)
6222797 .. 6223177
381 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 381 bp
ATGATTGGGGTTCATGGTGCTGCCATGACACACTTTATGTTCATGAAGCCTGGGTCTGTGTTTATCCAAGTTGTTCCCCTTGGCACTGAGTGGGCAGCCAAGGAGTATTATGGGGAACCTGCAAGGAAGCTCGGCTTAAAATACATCGGCTACCAAATTCTAACTCGGGAGAGCTCGTTATACGCCAAATATGATAAGGATGATCCTGTTCTTAGGGATCCAGAGAGTGTAAACAAAATGGGATGGGAATACACAAAGAAAATCTATCTTGATGGCCAAACTGTGAGATTAGACCTCGGAAGATTTCGGAAACGATTGGTTCGTGCTTACGGCTACATCTTCCACAGGTTGAACTATCACCCCCATCTTCAATCTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 map00514
Pfam Domains
Protein Families
DUF563

Protein Analysis

127

Amino Acids

14.76

Weight (kDa)

9.6

Isoelectric Point (pI)

34.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018886)

Species Orthologous Gene IDs
malus_domestica MD14G1074200.v1.1
pyrus_communis pycom14g06330
rosa_chinensis RchiOBHm_Chr3g0483211
rosa_laevigata RLG00000023296 RLG00000023298
rosa_multiflora Rmu_co8194502.1_g000001 Rmu_sc0007550.1_g000001
rosa_roxburghii Rroxscaffold_6G00398540
rosa_samantha Rh3BG290200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 127
AclWI GGATC 3 cut(s) 197, 212, 225
AcoI YGGCCR 1 cut(s) 274
AcsI RAATTY 1 cut(s) 156
AdeI CACNNNGTG 1 cut(s) 90
AgsI TTSAA 2 cut(s) 352, 371
AjnI CCWGG 1 cut(s) 49
AluBI AGCT 2 cut(s) 130, 174
AluI AGCT 2 cut(s) 130, 174
Alw21I GWGCWC 1 cut(s) 176
AlwI GGATC 3 cut(s) 197, 212, 225
Ama87I CYCGRG 1 cut(s) 165
AoxI GGCC 1 cut(s) 274
ApeKI GCWGC 2 cut(s) 20, 95
ApoI RAATTY 1 cut(s) 156
AsuHPI GGTGA 1 cut(s) 350
AvaI CYCGRG 1 cut(s) 165
BalI TGGCCA 1 cut(s) 276
BamHI GGATCC 1 cut(s) 217
BanII GRGCYC 1 cut(s) 176
Bbv12I GWGCWC 1 cut(s) 176
BbvI GCAGC 2 cut(s) 7, 107
BccI CCATC 3 cut(s) 237, 266, 372
BceAI ACGGC 1 cut(s) 346
BciT130I CCWGG 1 cut(s) 51
BfuAI ACCTGC 1 cut(s) 127
BisI GCNGC 2 cut(s) 21, 96
BlsI GCNGC 2 cut(s) 22, 97
Bme1390I CCNGG 1 cut(s) 51
BmeT110I CYCGRG 1 cut(s) 165
BmiI GGNNCC 2 cut(s) 117, 219
BmrFI CCNGG 1 cut(s) 51
BsaJI CCNNGG 4 cut(s) 50, 79, 99, 295
BsaXI ACNNNNNCTCC 2 cut(s) 161, 191
BseBI CCWGG 1 cut(s) 51
BseDI CCNNGG 4 cut(s) 50, 79, 99, 295
BseGI GGATG 2 cut(s) 205, 248
BseMII CTCAG 1 cut(s) 78
BseXI GCAGC 2 cut(s) 7, 107
BshFI GGCC 1 cut(s) 276
BsiHKAI GWGCWC 1 cut(s) 176
BsiHKCI CYCGRG 1 cut(s) 165
BsnI GGCC 1 cut(s) 276
BsoBI CYCGRG 1 cut(s) 165
Bsp1286I GDGCHC 1 cut(s) 176
Bsp143I GATC 2 cut(s) 202, 217
BspANI GGCC 1 cut(s) 276
BspCNI CTCAG 1 cut(s) 79
BspHI TCATGA 1 cut(s) 42
BspLI GGNNCC 2 cut(s) 117, 219
BspMI ACCTGC 1 cut(s) 127
BspPI GGATC 3 cut(s) 197, 212, 225
BssECI CCNNGG 4 cut(s) 50, 79, 99, 295
BssMI GATC 2 cut(s) 202, 217
BssT1I CCWWGG 2 cut(s) 79, 99
Bst2UI CCWGG 1 cut(s) 51
Bst4CI ACNGT 1 cut(s) 283
BstDEI CTNAG 3 cut(s) 87, 212, 375
BstF5I GGATG 2 cut(s) 205, 248
BstKTI GATC 2 cut(s) 205, 220
BstMBI GATC 2 cut(s) 202, 217
BstNI CCWGG 1 cut(s) 51
BstSCI CCNGG 1 cut(s) 49
BstV1I GCAGC 2 cut(s) 7, 107
BstX2I RGATCY 1 cut(s) 217
BstYI RGATCY 1 cut(s) 217
BsuRI GGCC 1 cut(s) 276
BtsCI GGATG 2 cut(s) 205, 248
BtsIMutI CAGTG 1 cut(s) 84
BveI ACCTGC 1 cut(s) 127
CciI TCATGA 1 cut(s) 42
CviAII CATG 3 cut(s) 14, 25, 43
CviJI RGCY 8 cut(s) 49, 98, 130, 135, 150, 174, 276, 333
CviKI_1 RGCY 8 cut(s) 49, 98, 130, 135, 150, 174, 276, 333
DdeI CTNAG 3 cut(s) 87, 212, 375
DpnI GATC 2 cut(s) 204, 219
DpnII GATC 2 cut(s) 202, 217
DraIII CACNNNGTG 1 cut(s) 90
EaeI YGGCCR 1 cut(s) 274
Ecl136II GAGCTC 1 cut(s) 174
Eco130I CCWWGG 2 cut(s) 79, 99
Eco24I GRGCYC 1 cut(s) 176
Eco53kI GAGCTC 1 cut(s) 174
Eco88I CYCGRG 1 cut(s) 165
EcoICRI GAGCTC 1 cut(s) 174
EcoRII CCWGG 1 cut(s) 49
EcoT14I CCWWGG 2 cut(s) 79, 99
EcoT38I GRGCYC 1 cut(s) 176
ErhI CCWWGG 2 cut(s) 79, 99
FaeI CATG 3 cut(s) 17, 28, 46
FaiI YATR 7 cut(s) 15, 26, 38, 44, 111, 181, 192
FalI AAGNNNNNCTT 2 cut(s) 119, 151
FatI CATG 3 cut(s) 13, 24, 42
Fnu4HI GCNGC 2 cut(s) 21, 96
FokI GGATG 2 cut(s) 212, 255
FriOI GRGCYC 1 cut(s) 176
Fsp4HI GCNGC 2 cut(s) 21, 96
GluI GCNGC 2 cut(s) 21, 96
HaeIII GGCC 1 cut(s) 276
Hin1II CATG 3 cut(s) 17, 28, 46
HphI GGTGA 1 cut(s) 350
Hpy166II GTNNAC 1 cut(s) 232
Hpy188I TCNGA 2 cut(s) 299, 309
Hpy188III TCNNGA 4 cut(s) 43, 167, 221, 269
Hpy8I GTNNAC 1 cut(s) 232
HpyCH4III ACNGT 1 cut(s) 283
HpyCH4V TGCA 1 cut(s) 122
HpyF3I CTNAG 3 cut(s) 87, 212, 375
Hsp92II CATG 3 cut(s) 17, 28, 46
Kzo9I GATC 2 cut(s) 202, 217
LpnPI CCDG 6 cut(s) 36, 63, 132, 219, 234, 331
Lsp1109I GCAGC 2 cut(s) 7, 107
MalI GATC 2 cut(s) 204, 219
MboI GATC 2 cut(s) 202, 217
MboII GAAGA 3 cut(s) 312, 331, 359
MflI RGATCY 1 cut(s) 217
MhlI GDGCHC 1 cut(s) 176
MlsI TGGCCA 1 cut(s) 276
MluCI AATT 1 cut(s) 156
MluNI TGGCCA 1 cut(s) 276
MnlI CCTC 1 cut(s) 305
Mox20I TGGCCA 1 cut(s) 276
MscI TGGCCA 1 cut(s) 276
MseI TTAA 1 cut(s) 137
Msp20I TGGCCA 1 cut(s) 276
MspR9I CCNGG 1 cut(s) 51
MvaI CCWGG 1 cut(s) 51
NdeII GATC 2 cut(s) 202, 217
NlaIII CATG 3 cut(s) 17, 28, 46
NlaIV GGNNCC 2 cut(s) 117, 219
NmeAIII GCCGAG 1 cut(s) 111
PagI TCATGA 1 cut(s) 42
PcsI WCGNNNNNNNCGW 1 cut(s) 319
PkrI GCNGC 2 cut(s) 22, 97
Psp124BI GAGCTC 1 cut(s) 176
Psp6I CCWGG 1 cut(s) 49
PspGI CCWGG 1 cut(s) 49
PspN4I GGNNCC 2 cut(s) 117, 219
PsuI RGATCY 1 cut(s) 217
SacI GAGCTC 1 cut(s) 176
SaqAI TTAA 1 cut(s) 137
SatI GCNGC 2 cut(s) 21, 96
Sau3AI GATC 2 cut(s) 202, 217
ScrFI CCNGG 1 cut(s) 51
SduI GDGCHC 1 cut(s) 176
SetI ASST 5 cut(s) 121, 132, 176, 297, 350
Sse9I AATT 1 cut(s) 156
SstI GAGCTC 1 cut(s) 176
StyD4I CCNGG 1 cut(s) 49
StyI CCWWGG 2 cut(s) 79, 99
TaaI ACNGT 1 cut(s) 283
TasI AATT 1 cut(s) 156
Tru1I TTAA 1 cut(s) 137
Tru9I TTAA 1 cut(s) 137
TscAI CASTG 1 cut(s) 91
TseI GCWGC 2 cut(s) 20, 95
TspDTI ATGAA 2 cut(s) 31, 59
TspRI CASTG 1 cut(s) 91
XapI RAATTY 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.