pycom14g06360

Protein of unknown function, DUF617

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0014282
Physical Location & Seq
Reverse (-)
6270013 .. 6270852
840 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 840 bp
ATGAAACACCAAGAGCTCAGCCTCCACAGAAGCACCAGTACTACTTCCATATCAAGAAGCACTTCAAGAAAGATCATCCCATCCAACCACTTCAGATCTTCACCATCAACTCTCCCACTTAACCATCTTCAAATCGACGACGTCCCCAACAACCTTTTGATTCCGAAACACCACTCTCCAGTCTCCTTCCACCACATATCCAAAACCCAAACAAAGATCACCTCCCTCCTCCGCTCTTTCCTCAGATTCCTCTCCTTCCCCACCATTATCCCGACATGCAAGTGGCTCGCCCTCCCCTCCAACCTCTCCGTCACCCCCTCCCTAGGCCGAAAAGTCACCGGCACGCTCTTTGGAAACCGCCGTGGTCACGTCAGCTTCGCCGTTCAGCTGGACACCCAGTCGGATCCTGTCTTGCTTCTCGAGCTAGCCATGTCAACGTCTTCACTCGTTAAAGAGATGTCTTCGGGGCTCGTGCGCATTGCTCTCGAGTCTGAAAAGCAACAGAGTTCCGGCCGTGCAGGTATGAATGGCCGGAAGCTGTTTCAGGAGCCTTCATGGACTATGTATTGTAATGGGAGGAAGTGTGGGTATGCGGCGACTCGCACGTGCGGAGAGTCAGACTGGCACGTGCTGCGCACTGTTCGGAGTGTTTCGGTCGGAGCCGGCGTGATTCCGGTGTTGGACGACGGAAAAAAGGGTGGCGCGTCTGAAGGTGAGTTGCTTTATATGAGAGCTAGGTTTGAACGAGTTGTGGGAAGCCGTGACTCGGAGGCCTTTTACATGTTGAACCCTGAGGGTAATGGAGGTCCTGAACTCAGTATCTTTTTACTCAGAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF617

Protein Analysis

280

Amino Acids

30.82

Weight (kDa)

9.97

Isoelectric Point (pI)

54.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF617 PF04759 112 - 278 7.4e-70 Protein of unknown function, DUF617
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014282)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41660
fragaria_vesca FvH4_6g24150
malus_domestica MD12G1076100.v1.1 MD14G1074600.v1.1
prunus_persica Prupe.7G056000_v2.0.a1
pyrus_communis pycom14g06360
rosa_chinensis RchiOBHm_Chr3g0483021
rosa_laevigata RLG00000023306
rosa_multiflora Rmu_sc0001098.1_g000001 Rmu_sc0001098.1_g000002
rosa_roxburghii Rroxscaffold_6G00398730
rosa_rugosa Rorug03G0203100
rosa_samantha Rh3AG251700 Rh3BG288200 Rh3CG285700 Rh3DG281400
rosa_wichuraiana Rw3G022770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 144
Acc16I TGCGCA 2 cut(s) 476, 635
Acc36I ACCTGC 1 cut(s) 509
AccBSI CCGCTC 1 cut(s) 234
AccII CGCG 1 cut(s) 704
AciI CCGC 4 cut(s) 232, 358, 593, 609
AclWI GGATC 2 cut(s) 398, 411
AcoI YGGCCR 2 cut(s) 511, 529
AcuI CTGAAG 2 cut(s) 76, 729
AcvI CACGTG 2 cut(s) 606, 628
AcyI GRCGYC 1 cut(s) 141
AfaI GTAC 1 cut(s) 40
AfiI CCNNNNNNNGG 2 cut(s) 323, 766
AflIII ACRYGT 1 cut(s) 780
AgsI TTSAA 4 cut(s) 66, 131, 743, 787
AhdI GACNNNNNGTC 1 cut(s) 397
AjiI CACGTC 1 cut(s) 370
AluBI AGCT 6 cut(s) 16, 375, 388, 424, 538, 734
AluI AGCT 6 cut(s) 16, 375, 388, 424, 538, 734
Alw21I GWGCWC 1 cut(s) 18
Alw26I GTCTC 1 cut(s) 187
AlwI GGATC 2 cut(s) 398, 411
Ama87I CYCGRG 2 cut(s) 419, 485
AoxI GGCC 4 cut(s) 325, 511, 529, 771
ApeKI GCWGC 1 cut(s) 631
Asp700I GAANNNNTTC 1 cut(s) 61
AspA2I CCTAGG 1 cut(s) 322
AspLEI GCGC 3 cut(s) 477, 636, 704
AspS9I GGNCC 1 cut(s) 806
AsuHPI GGTGA 5 cut(s) 93, 211, 304, 328, 725
AsuNHI GCTAGC 1 cut(s) 424
AvaI CYCGRG 2 cut(s) 419, 485
AvaII GGWCC 1 cut(s) 806
AvrII CCTAGG 1 cut(s) 322
AxyI CCTNAGG 1 cut(s) 792
BamHI GGATCC 1 cut(s) 403
BanII GRGCYC 2 cut(s) 18, 471
BarI GAAGNNNNNNTAC 2 cut(s) 22, 54
BauI CACGAG 1 cut(s) 470
BbrPI CACGTG 2 cut(s) 606, 628
BbsI GAAGAC 2 cut(s) 432, 453
Bbv12I GWGCWC 1 cut(s) 18
BbvI GCAGC 1 cut(s) 618
BccI CCATC 3 cut(s) 88, 112, 132
BceAI ACGGC 4 cut(s) 345, 365, 498, 744
BcgI CGANNNNNNTGC 4 cut(s) 268, 302, 466, 500
BcoDI GTCTC 1 cut(s) 187
BfaI CTAG 3 cut(s) 323, 425, 735
BfuAI ACCTGC 1 cut(s) 509
BglII AGATCT 1 cut(s) 95
BisI GCNGC 2 cut(s) 594, 632
BlnI CCTAGG 1 cut(s) 322
BlpI GCTNAGC 1 cut(s) 17
BlsI GCNGC 2 cut(s) 595, 633
BmcAI AGTACT 1 cut(s) 40
Bme18I GGWCC 1 cut(s) 806
BmeRI GACNNNNNGTC 1 cut(s) 397
BmeT110I CYCGRG 2 cut(s) 419, 485
BmgBI CACGTC 1 cut(s) 370
BmgT120I GGNCC 1 cut(s) 806
BmiI GGNNCC 3 cut(s) 405, 549, 661
BmrI ACTGGG 1 cut(s) 391
BmtI GCTAGC 1 cut(s) 428
BmuI ACTGGG 1 cut(s) 391
BpiI GAAGAC 2 cut(s) 432, 453
BpmI CTGGAG 1 cut(s) 162
Bpu1102I GCTNAGC 1 cut(s) 17
BsaAI YACGTR 2 cut(s) 606, 628
BsaHI GRCGYC 1 cut(s) 141
BsaJI CCNNGG 2 cut(s) 322, 361
BsaWI WCCGGW 1 cut(s) 673
BsaXI ACNNNNNCTCC 4 cut(s) 568, 598, 651, 681
Bsc4I CCNNNNNNNGG 2 cut(s) 323, 766
Bse118I RCCGGY 2 cut(s) 338, 662
Bse1I ACTGG 4 cut(s) 36, 179, 397, 626
Bse21I CCTNAGG 1 cut(s) 792
Bse3DI GCAATG 1 cut(s) 477
BseDI CCNNGG 2 cut(s) 322, 361
BseGI GGATG 2 cut(s) 75, 80
BseLI CCNNNNNNNGG 2 cut(s) 323, 766
BseMI GCAATG 1 cut(s) 477
BseMII CTCAG 4 cut(s) 31, 256, 783, 829
BseNI ACTGG 4 cut(s) 36, 179, 397, 626
BseRI GAGGAG 1 cut(s) 218
BseX3I CGGCCG 1 cut(s) 511
BseXI GCAGC 1 cut(s) 618
BsgI GTGCAG 1 cut(s) 537
Bsh1236I CGCG 1 cut(s) 704
Bsh1285I CGRYCG 2 cut(s) 514, 657
BshFI GGCC 4 cut(s) 327, 513, 531, 773
BsiEI CGRYCG 2 cut(s) 514, 657
BsiHKAI GWGCWC 1 cut(s) 18
BsiHKCI CYCGRG 2 cut(s) 419, 485
BsiSI CCGG 5 cut(s) 339, 510, 532, 663, 674
BslFI GGGAC 1 cut(s) 128
BslI CCNNNNNNNGG 2 cut(s) 323, 766
BsmAI GTCTC 1 cut(s) 187
BsmFI GGGAC 1 cut(s) 128
BsnI GGCC 4 cut(s) 327, 513, 531, 773
BsoBI CYCGRG 2 cut(s) 419, 485
Bsp1286I GDGCHC 2 cut(s) 18, 471
Bsp143I GATC 4 cut(s) 72, 95, 216, 403
Bsp1720I GCTNAGC 1 cut(s) 17
BspACI CCGC 4 cut(s) 232, 358, 593, 609
BspANI GGCC 4 cut(s) 327, 513, 531, 773
BspCNI CTCAG 4 cut(s) 30, 255, 784, 828
BspFNI CGCG 1 cut(s) 704
BspLI GGNNCC 3 cut(s) 405, 549, 661
BspMI ACCTGC 1 cut(s) 509
BspOI GCTAGC 1 cut(s) 428
BspPI GGATC 2 cut(s) 398, 411
BsrBI CCGCTC 1 cut(s) 234
BsrDI GCAATG 1 cut(s) 477
BsrFI RCCGGY 2 cut(s) 338, 662
BsrI ACTGG 4 cut(s) 36, 179, 397, 626
BssAI RCCGGY 2 cut(s) 338, 662
BssECI CCNNGG 2 cut(s) 322, 361
BssMI GATC 4 cut(s) 72, 95, 216, 403
BssNI GRCGYC 1 cut(s) 141
BssSI CACGAG 1 cut(s) 470
BssT1I CCWWGG 1 cut(s) 322
Bst2BI CACGAG 1 cut(s) 470
Bst4CI ACNGT 1 cut(s) 640
BstACI GRCGYC 1 cut(s) 141
BstAPI GCANNNNNTGC 1 cut(s) 631
BstBAI YACGTR 2 cut(s) 606, 628
BstC8I GCNNGC 4 cut(s) 288, 344, 426, 664
BstDEI CTNAG 5 cut(s) 17, 242, 792, 815, 830
BstDSI CCRYGG 1 cut(s) 361
BstF5I GGATG 2 cut(s) 75, 80
BstFNI CGCG 1 cut(s) 704
BstHHI GCGC 3 cut(s) 477, 636, 704
BstKTI GATC 4 cut(s) 75, 98, 219, 406
BstMAI GTCTC 1 cut(s) 187
BstMBI GATC 4 cut(s) 72, 95, 216, 403
BstMCI CGRYCG 2 cut(s) 514, 657
BstMWI GCNNNNNNNGC 2 cut(s) 421, 631
BstNSI RCATGY 2 cut(s) 279, 784
BstUI CGCG 1 cut(s) 704
BstV1I GCAGC 1 cut(s) 618
BstV2I GAAGAC 2 cut(s) 432, 453
BstX2I RGATCY 2 cut(s) 95, 403
BstYI RGATCY 2 cut(s) 95, 403
BstZI CGGCCG 1 cut(s) 511
Bsu36I CCTNAGG 1 cut(s) 792
BsuRI GGCC 4 cut(s) 327, 513, 531, 773
BtgI CCRYGG 1 cut(s) 361
BtrI CACGTC 1 cut(s) 370
BtsCI GGATG 2 cut(s) 75, 80
BtsIMutI CAGTG 1 cut(s) 636
BveI ACCTGC 1 cut(s) 509
Cac8I GCNNGC 4 cut(s) 288, 344, 426, 664
CfoI GCGC 3 cut(s) 477, 636, 704
Cfr10I RCCGGY 2 cut(s) 338, 662
Cfr13I GGNCC 1 cut(s) 806
CseI GACGC 1 cut(s) 693
Csp6I GTAC 1 cut(s) 39
CviAII CATG 4 cut(s) 276, 430, 555, 781
CviQI GTAC 1 cut(s) 39
DdeI CTNAG 5 cut(s) 17, 242, 792, 815, 830
DpnI GATC 4 cut(s) 74, 97, 218, 405
DpnII GATC 4 cut(s) 72, 95, 216, 403
DriI GACNNNNNGTC 1 cut(s) 397
EaeI YGGCCR 2 cut(s) 511, 529
EagI CGGCCG 1 cut(s) 511
Eam1105I GACNNNNNGTC 1 cut(s) 397
Ecl136II GAGCTC 1 cut(s) 16
EclXI CGGCCG 1 cut(s) 511
Eco130I CCWWGG 1 cut(s) 322
Eco147I AGGCCT 1 cut(s) 773
Eco24I GRGCYC 2 cut(s) 18, 471
Eco47I GGWCC 1 cut(s) 806
Eco52I CGGCCG 1 cut(s) 511
Eco53kI GAGCTC 1 cut(s) 16
Eco57I CTGAAG 2 cut(s) 76, 729
Eco72I CACGTG 2 cut(s) 606, 628
Eco81I CCTNAGG 1 cut(s) 792
Eco88I CYCGRG 2 cut(s) 419, 485
EcoICRI GAGCTC 1 cut(s) 16
EcoO109I RGGNCCY 1 cut(s) 806
EcoT14I CCWWGG 1 cut(s) 322
EcoT38I GRGCYC 2 cut(s) 18, 471
ErhI CCWWGG 1 cut(s) 322
FaeI CATG 4 cut(s) 279, 433, 558, 784
FalI AAGNNNNNCTT 2 cut(s) 46, 78
FaqI GGGAC 1 cut(s) 128
FatI CATG 4 cut(s) 275, 429, 554, 780
Fnu4HI GCNGC 2 cut(s) 594, 632
FokI GGATG 2 cut(s) 62, 67
FriOI GRGCYC 2 cut(s) 18, 471
Fsp4HI GCNGC 2 cut(s) 594, 632
FspAI RTGCGCAY 1 cut(s) 476
FspBI CTAG 3 cut(s) 323, 425, 735
FspI TGCGCA 2 cut(s) 476, 635
GlaI GCGC 3 cut(s) 476, 635, 703
GluI GCNGC 2 cut(s) 594, 632
GsuI CTGGAG 1 cut(s) 162
HaeIII GGCC 4 cut(s) 327, 513, 531, 773
HapII CCGG 5 cut(s) 339, 510, 532, 663, 674
HgaI GACGC 1 cut(s) 693
HhaI GCGC 3 cut(s) 477, 636, 704
Hin1I GRCGYC 1 cut(s) 141
Hin1II CATG 4 cut(s) 279, 433, 558, 784
Hin6I GCGC 3 cut(s) 475, 634, 702
HinP1I GCGC 3 cut(s) 475, 634, 702
HincII GTYRAC 1 cut(s) 435
HindII GTYRAC 1 cut(s) 435
HinfI GANTC 7 cut(s) 160, 246, 488, 598, 614, 670, 764
HpaII CCGG 5 cut(s) 339, 510, 532, 663, 674
HphI GGTGA 5 cut(s) 93, 211, 304, 328, 725
Hpy166II GTNNAC 1 cut(s) 435
Hpy188III TCNNGA 7 cut(s) 54, 66, 271, 419, 485, 545, 809
Hpy8I GTNNAC 1 cut(s) 435
Hpy99I CGWCG 3 cut(s) 140, 143, 689
HpyAV CCTTC 4 cut(s) 196, 265, 561, 704
HpyCH4III ACNGT 1 cut(s) 640
HpyCH4IV ACGT 5 cut(s) 141, 369, 437, 605, 627
HpyCH4V TGCA 2 cut(s) 279, 518
HpyF10VI GCNNNNNNNGC 2 cut(s) 421, 631
HpyF3I CTNAG 5 cut(s) 17, 242, 792, 815, 830
HpySE526I ACGT 5 cut(s) 141, 369, 437, 605, 627
Hsp92I GRCGYC 1 cut(s) 141
Hsp92II CATG 4 cut(s) 279, 433, 558, 784
HspAI GCGC 3 cut(s) 475, 634, 702
KroI GCCGGC 1 cut(s) 662
KroNI GCCGGC 1 cut(s) 664
Kzo9I GATC 4 cut(s) 72, 95, 216, 403
LmnI GCTCC 2 cut(s) 547, 659
Lsp1109I GCAGC 1 cut(s) 618
MaeI CTAG 3 cut(s) 323, 425, 735
MaeII ACGT 5 cut(s) 141, 369, 437, 605, 627
MaeIII GTNAC 4 cut(s) 310, 334, 365, 761
MalI GATC 4 cut(s) 74, 97, 218, 405
MbiI CCGCTC 1 cut(s) 234
MboI GATC 4 cut(s) 72, 95, 216, 403
MboII GAAGA 4 cut(s) 90, 119, 432, 453
MflI RGATCY 2 cut(s) 95, 403
MhlI GDGCHC 2 cut(s) 18, 471
MlyI GAGTC 4 cut(s) 497, 592, 623, 758
MmeI TCCRAC 5 cut(s) 108, 324, 381, 637, 660
MroNI GCCGGC 1 cut(s) 662
MroXI GAANNNNTTC 1 cut(s) 61
MseI TTAA 2 cut(s) 120, 450
MslI CAYNNNNRTG 1 cut(s) 280
MspA1I CMGCKG 1 cut(s) 388
MspI CCGG 5 cut(s) 339, 510, 532, 663, 674
MvnI CGCG 1 cut(s) 704
MwoI GCNNNNNNNGC 2 cut(s) 421, 631
NaeI GCCGGC 1 cut(s) 664
NdeII GATC 4 cut(s) 72, 95, 216, 403
NgoMIV GCCGGC 1 cut(s) 662
NheI GCTAGC 1 cut(s) 424
NlaIII CATG 4 cut(s) 279, 433, 558, 784
NlaIV GGNNCC 3 cut(s) 405, 549, 661
NmuCI GTSAC 4 cut(s) 310, 334, 365, 761
NsbI TGCGCA 2 cut(s) 476, 635
NspI RCATGY 2 cut(s) 279, 784
PaeR7I CTCGAG 2 cut(s) 419, 485
PceI AGGCCT 1 cut(s) 773
PciI ACATGT 1 cut(s) 780
PcsI WCGNNNNNNNCGW 1 cut(s) 663
PdiI GCCGGC 1 cut(s) 664
PdmI GAANNNNTTC 1 cut(s) 61
PfeI GAWTC 3 cut(s) 160, 246, 670
PflFI GACNNNGTC 1 cut(s) 140
PkrI GCNGC 2 cut(s) 595, 633
PleI GAGTC 4 cut(s) 496, 592, 622, 758
PmaCI CACGTG 2 cut(s) 606, 628
PmlI CACGTG 2 cut(s) 606, 628
PpsI GAGTC 4 cut(s) 496, 592, 622, 758
Ppu21I YACGTR 2 cut(s) 606, 628
PpuMI RGGWCCY 1 cut(s) 806
PscI ACATGT 1 cut(s) 780
Psp124BI GAGCTC 1 cut(s) 18
Psp5II RGGWCCY 1 cut(s) 806
PspCI CACGTG 2 cut(s) 606, 628
PspN4I GGNNCC 3 cut(s) 405, 549, 661
PspPI GGNCC 1 cut(s) 806
PspPPI RGGWCCY 1 cut(s) 806
PsuI RGATCY 2 cut(s) 95, 403
PsyI GACNNNGTC 1 cut(s) 140
PvuII CAGCTG 1 cut(s) 388
RsaI GTAC 1 cut(s) 40
RsaNI GTAC 1 cut(s) 39
RseI CAYNNNNRTG 1 cut(s) 280
SacI GAGCTC 1 cut(s) 18
SaqAI TTAA 2 cut(s) 120, 450
SatI GCNGC 2 cut(s) 594, 632
Sau3AI GATC 4 cut(s) 72, 95, 216, 403
Sau96I GGNCC 1 cut(s) 806
ScaI AGTACT 1 cut(s) 40
SchI GAGTC 4 cut(s) 497, 592, 623, 758
SduI GDGCHC 2 cut(s) 18, 471
Sfr274I CTCGAG 2 cut(s) 419, 485
SinI GGWCC 1 cut(s) 806
SlaI CTCGAG 2 cut(s) 419, 485
SmiMI CAYNNNNRTG 1 cut(s) 280
SmlI CTYRAG 2 cut(s) 419, 485
SmoI CTYRAG 2 cut(s) 419, 485
SseBI AGGCCT 1 cut(s) 773
SsiI CCGC 4 cut(s) 232, 358, 593, 609
SspMI CTAG 3 cut(s) 323, 425, 735
SstI GAGCTC 1 cut(s) 18
StuI AGGCCT 1 cut(s) 773
StyI CCWWGG 1 cut(s) 322
TaaI ACNGT 1 cut(s) 640
TaiI ACGT 5 cut(s) 144, 372, 440, 608, 630
TaqI TCGA 3 cut(s) 135, 420, 486
TaqII GACCGA 1 cut(s) 643
TatI WGTACW 1 cut(s) 38
TauI GCSGC 1 cut(s) 596
TfiI GAWTC 3 cut(s) 160, 246, 670
Tru1I TTAA 2 cut(s) 120, 450
Tru9I TTAA 2 cut(s) 120, 450
TscAI CASTG 1 cut(s) 643
TseFI GTSAC 4 cut(s) 310, 334, 365, 761
TseI GCWGC 1 cut(s) 631
Tsp45I GTSAC 4 cut(s) 310, 334, 365, 761
TspDTI ATGAA 3 cut(s) 17, 539, 543
TspGWI ACGGA 2 cut(s) 298, 702
TspRI CASTG 1 cut(s) 643
Tth111I GACNNNGTC 1 cut(s) 140
VpaK11BI GGWCC 1 cut(s) 806
XceI RCATGY 2 cut(s) 279, 784
XhoI CTCGAG 2 cut(s) 419, 485
XmaJI CCTAGG 1 cut(s) 322
XmnI GAANNNNTTC 1 cut(s) 61
XspI CTAG 3 cut(s) 323, 425, 735
ZraI GACGTC 1 cut(s) 142
ZrmI AGTACT 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.