pycom14g09740

N-terminal C2 in EEIG1 and EHBP1 proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0007404
Physical Location & Seq
Forward (+)
11929376 .. 11933787
4412 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3333 bp
ATGTTCAAGTCGGCGCGATGGAGAACCAACAAGAACACGATCAAGGAAGTCTTCAAATTGCAGTTCCATGCAACTCAGGTGACACAGTTGGGTGGGGACGCATTGCTGGTTTCTGTGATTCCCGTGGATGTGGGGAAGCCAACTGTGAAATCAGAGAAAGCTACAGTTCGAGACGGGAGCTGTCGATGGGAAAATCCGATCTATGAAACAATTAAATTCGTCCGTGAGCCAAGAACTGGGAAGATCAAAGAGAGTGTCTACAACTTTGTTGTCTCAAGTGGATCATCAAAGGGTAGTGTTCTTGGGGAAGTTTCTGTCGATTTTACTGACTATGCCGAGGCTACTAAATCTTCCTGCGTCGCTCTTCCCCTTAAGAATTCAAATTCTAATGCAATTATGCATGTTACCATTCAAAGGTTGCAAGCAAATGTTGATCTGAGAGAGGTAGAATGTGGTGAAGAGGCAAATGTTAACTCCGAAGATAGAAGCTTCAAGAACCTCTTAAGCAATAAGGACACTGATGAAAGCGTTTCAATTGATGAGGGAATTAATAGAACCACCCAAATTGCTGAGCTCAACCGCAGAGCATCTATCGGATCGGACATTACATTGTCAAGTTCTGACAGTGGCTCTGGACTTGGTACTCCACGAGAACTTGGATCAAGAGATCCTTCCAACTTTCCGTCGTCTCTGAGTCACACCTCAGTACAACATAAACCTGATATTTATGCCCGAACAAATATCTATGACGAGCATCAGCGGTTACAATGGGCATGGTCTGCAGACTCAGAACATGGAGCAAGTACTGATGGTTCAACAAAGAGTTCTCATGAAACCCTTCCAAGGGAACAGTCTCAACAGTCTTCAGATGACGACATTGAAAAGCTAAAGGCGGAGCTTGTTGTTCTAGCTAGACAGGCCGATATGTCAGAGCTAGAATTACAAACATTAAGGAAGCAGATAGTAAAAGAGACCAAAAGGGGGCAGGATCTTTCGAGAGAAGTCATTAGCTTGAAAGATGAACGAGATTCTTTCAAGGCAGAATGTGAGAAACTAAAGTCCTTTTGGAAACGTGTGGATGATGCAAAAGTTAAAAACACGTTCCAGTTAGAAGGTGGTGATCTACGGACTCTTGTTGATGAAATCAGACAAGAACTGAGTTGTGAGAAGGACATGACTTTCAATCTCCGCTTACAGCTCCAGAAAACGCAAGAATCAAATTCTGATTTAATCCTTGCTGTACGGGATCTGGAGGAAATTTTGGAGCAGAAGAATAGTGAAATAGCAAATCCTTCCAACAGACCAGAATCCTGCGGTTATGCTGCAGGGTTGAAGGAAACCATCTCGAAAGGTGGGACAAGTGAGGATGAGGAGCAGATGGAGCTGGAGGATCTTGTTAAGGACCGCAGCAATGCCACGGAAACACACCTGCTCGAGAGACAGATTGCCGAACTTTATGGTGAAATAGAAATCTATAGGCGAGACAAAGATGAGCTCGAGATGCAAATGGAGCAGCTTGCACTTGACTATGAGATATTGAAACAGGAAAACCATGACATCTCATATAAACTAGAGCAAAGCCAACTGCAAGAACAACTTAAGATGCAGTACGAATGCTTGTCCCCCTCTGCTATCATAAATGAACTTGAATACCAAGTTGAGGATTTGGAAACGGAATTGAAGAAGCAGTCTGAAGATTTTTCAAATTCATTGGCTACCATAAAGGAACTTGAATCCCATATCAAAAGCTTGGAGGAAGAACTAAAGAAGCAGGCACAAGTATATGAGGCTGATTTAGAGGCTGTAACATGTGACAAAGTTGCGGGTTTGGAAACAGAACTAAAGAAGCAGTCTGAAGATTTTTCAAATTCGTTGGCTACCATAAAGGAACTTGAATTCCATCTCAAAAGCTTGGAGGAAGAACTGGAGAAACAGGTGCAAGTATTTGAAGCTGATTTAGAGGCCGTAACATGTGCCAAAGTTGAGCAGGAGCAAAGAGCCATCCGAGCAGAGGACACACTGCGAAAAACTAGATCAAAAAATGCTAATACAGCTGAGAGGCTTCAGGAAGAATTCCGAAGACTCTCTGTTCAAATGGCCTCGACATTTGATGCAAATGAAAAGGTAGCTCTGAAAGCAATGACAGAAGCCAGTGAACTGTGTGGGCAGAAGAGTCACCTAGAAGAACTGGTCCAAAAAATGAAGGACGAGCTCCAGGAAGTTAGAAATGATTATGAGGCAAGATTGCAGAAGCTCTCGAACCACTTAGACGAGAAAACCAAAGAGATGGAAGAGATTTTACTGGAAATGGACAACAATTACAAGCAGCTCGACCATCAGCAGAAACAAGAGGAAGAAGTTAAGGGAAATCTCTCCCAGGTGATCTTACAACTCAAATCTCAGATTCAAAGGCTTACAGCAGAGAATAACAGCCTTTCTGAGCAAGTGGAGGAAAGCAAAAATTTGAGAGCTGATTTGGAACAAGTGAAGAAATCAATCGAGGAAGCTGAGATGCGGGCACAAAGAGGAAATGCAGAAAGAAATGAGCTGGTAAGTAAAATTTCTACTCTTAAGAAGGAAGCAGAGAAGTCATTGGAGGATTTGAATACAATGCGGCATCTCAAAGATGAAAAGGAGGTGACAGTTGGACTCCTAAAATCCGAGATAGAGGAGCTTAAAGCTCGGTGTAAAGACTTTAAACATGCTATATACGAGGATGAAGTTGAGAAAGAGAAACTTAAAAAGCAGGTATTCCAGCTTAAGGCTGACCTGAAAAAGAAGGAAGATGCGCTCACTACCGCTGAAAAGAAACTCAAGGAAAGCAACGGGTGCGCAATAGTTTCTGATGGAATTAAAACTACTCAGAGAAGCATCAAGTCTTTGCAAATTGCTCGTGGTTCGAAAGAAGTTGCATGTATGAAAGAGAGAATAAAATTGCTCGAGGGACAAATAAAATCGAAGGAAGCTGCTTTGGGAACCTCAACTGCTTCATTTTTGGAGAAGGAAAAGGATCTCCAGAACATAATCGAAGAATTAGAGAGCACGGTGGAAGAAATAAATCAGAATAGTGCATCTCAAAAGGTAACTGAAGATGCCGGTAGTGATAAAATTGCATCAGAACAAGAACCGAGACTTTTCACTGCTGATAACAGATACAGTGACTTATTAACTGAATTAGCATCATTGAAAGAGAGGAATATATCAATGGAAAGTGAACTAAAAGAGATGCAAGAGAGATATTCAGAAATAAGTCTCAAATTTGCAGAGGTCGAAGGTGAAAGACAACAGCTCGTAATGACAGTACGCATCCTCAAAAATTCCAAGAGGAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04530 map04530
Pfam Domains
Protein Families
NT-C2

Protein Analysis

1111

Amino Acids

126.45

Weight (kDa)

5.01

Isoelectric Point (pI)

47.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 10 - 144 1.1e-22 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1437
Acc16I TGCGCA 1 cut(s) 2833
Acc36I ACCTGC 2 cut(s) 1437, 2737
AccB7I CCANNNNNTGG 1 cut(s) 236
AccI GTMKAC 1 cut(s) 258
AccII CGCG 1 cut(s) 16
AclWI GGATC 8 cut(s) 289, 604, 662, 667, 996, 1254, 1398, 3018
AcuI CTGAAG 5 cut(s) 849, 1713, 1875, 2048, 3108
AfaI GTAC 6 cut(s) 643, 708, 805, 1242, 1610, 3303
AfiI CCNNNNNNNGG 8 cut(s) 236, 414, 843, 844, 981, 1660, 2011, 2761
AflII CTTAAG 5 cut(s) 371, 502, 1598, 2570, 2759
AflIII ACRYGT 4 cut(s) 1072, 1098, 1808, 1970
AjnI CCWGG 2 cut(s) 2214, 2376
AloI GAACNNNNNNTCC 2 cut(s) 1881, 1913
Alw21I GWGCWC 4 cut(s) 576, 1497, 2214, 3044
Alw26I GTCTC 9 cut(s) 165, 277, 693, 858, 965, 1432, 1476, 3124, 3257
AlwI GGATC 8 cut(s) 289, 604, 662, 667, 996, 1254, 1398, 3018
Ama87I CYCGRG 3 cut(s) 1433, 1496, 2939
AoxI GGCC 3 cut(s) 918, 1962, 2097
ApeKI GCWGC 5 cut(s) 1322, 1407, 1513, 2326, 2966
ArsI GACNNNNNNTTYG 2 cut(s) 240, 272
AseI ATTAAT 1 cut(s) 549
Asp700I GAANNNNTTC 1 cut(s) 837
AspLEI GCGC 3 cut(s) 16, 2791, 2834
AspS9I GGNCC 2 cut(s) 1402, 2191
AsuHPI GGTGA 8 cut(s) 91, 467, 1130, 1472, 2168, 2392, 2650, 3287
AsuII TTCGAA 1 cut(s) 2900
AvaI CYCGRG 3 cut(s) 1433, 1496, 2939
AvaII GGWCC 2 cut(s) 1402, 2191
BaeGI GKGCMC 1 cut(s) 2521
BanII GRGCYC 3 cut(s) 576, 1497, 2214
BarI GAAGNNNNNNTAC 2 cut(s) 2283, 2315
BauI CACGAG 2 cut(s) 648, 2892
BbsI GAAGAC 3 cut(s) 43, 855, 2086
Bbv12I GWGCWC 4 cut(s) 576, 1497, 2214, 3044
BbvI GCAGC 5 cut(s) 1309, 1419, 1525, 2338, 2953
BceAI ACGGC 1 cut(s) 1949
BcgI CGANNNNNNTGC 2 cut(s) 2873, 2907
BciT130I CCWGG 2 cut(s) 2216, 2378
BcoDI GTCTC 9 cut(s) 165, 277, 693, 858, 965, 1432, 1476, 3124, 3257
BfaI CTAG 6 cut(s) 908, 912, 935, 1571, 2031, 2180
BfmI CTRYAG 4 cut(s) 162, 780, 1323, 1474
BfrI CTTAAG 5 cut(s) 371, 502, 1598, 2570, 2759
BfuAI ACCTGC 2 cut(s) 1437, 2737
BisI GCNGC 6 cut(s) 1323, 1408, 1514, 2327, 2615, 2967
BlpI GCTNAGC 1 cut(s) 570
BlsI GCNGC 6 cut(s) 1324, 1409, 1515, 2328, 2616, 2968
BmcAI AGTACT 1 cut(s) 805
Bme1390I CCNGG 2 cut(s) 2216, 2378
Bme18I GGWCC 2 cut(s) 1402, 2191
BmeT110I CYCGRG 3 cut(s) 1433, 1496, 2939
BmgT120I GGNCC 2 cut(s) 1402, 2191
BmiI GGNNCC 1 cut(s) 2977
BmrFI CCNGG 2 cut(s) 2216, 2378
BmrI ACTGGG 1 cut(s) 246
BmuI ACTGGG 1 cut(s) 246
BpiI GAAGAC 3 cut(s) 43, 855, 2086
BpmI CTGGAG 6 cut(s) 1184, 1271, 1406, 1946, 2198, 2999
Bpu1102I GCTNAGC 1 cut(s) 570
Bpu14I TTCGAA 1 cut(s) 2900
BpuEI CTTGAG 2 cut(s) 259, 2798
BsaI GGTCTC 1 cut(s) 965
BsaJI CCNNGG 5 cut(s) 123, 336, 842, 1416, 2376
Bsc4I CCNNNNNNNGG 8 cut(s) 236, 414, 843, 844, 981, 1660, 2011, 2761
Bse118I RCCGGY 1 cut(s) 3095
Bse1I ACTGG 6 cut(s) 241, 1105, 1929, 2151, 2193, 2307
Bse3DI GCAATG 3 cut(s) 101, 1417, 2145
BseBI CCWGG 2 cut(s) 2216, 2378
BseDI CCNNGG 5 cut(s) 123, 336, 842, 1416, 2376
BseGI GGATG 6 cut(s) 133, 1084, 1372, 2001, 2722, 3306
BseLI CCNNNNNNNGG 8 cut(s) 236, 414, 843, 844, 981, 1660, 2011, 2761
BseMI GCAATG 3 cut(s) 101, 1417, 2145
BseNI ACTGG 6 cut(s) 241, 1105, 1929, 2151, 2193, 2307
BseRI GAGGAG 2 cut(s) 1385, 2684
BseSI GKGCMC 1 cut(s) 2521
BseXI GCAGC 5 cut(s) 1309, 1419, 1525, 2338, 2953
Bsh1236I CGCG 1 cut(s) 16
BshFI GGCC 3 cut(s) 920, 1964, 2099
BsiHKAI GWGCWC 4 cut(s) 576, 1497, 2214, 3044
BsiHKCI CYCGRG 3 cut(s) 1433, 1496, 2939
BsiSI CCGG 1 cut(s) 3096
BslFI GGGAC 4 cut(s) 110, 1369, 1606, 2958
BslI CCNNNNNNNGG 8 cut(s) 236, 414, 843, 844, 981, 1660, 2011, 2761
BsmAI GTCTC 9 cut(s) 165, 277, 693, 858, 965, 1432, 1476, 3124, 3257
BsmBI CGTCTC 2 cut(s) 165, 693
BsmFI GGGAC 4 cut(s) 110, 1369, 1606, 2958
BsmI GAATGC 1 cut(s) 1619
BsnI GGCC 3 cut(s) 920, 1964, 2099
Bso31I GGTCTC 1 cut(s) 965
BsoBI CYCGRG 3 cut(s) 1433, 1496, 2939
Bsp119I TTCGAA 1 cut(s) 2900
Bsp1286I GDGCHC 5 cut(s) 576, 1497, 2214, 2521, 3044
Bsp1720I GCTNAGC 1 cut(s) 570
BspANI GGCC 3 cut(s) 920, 1964, 2099
BspFNI CGCG 1 cut(s) 16
BspHI TCATGA 1 cut(s) 829
BspLI GGNNCC 1 cut(s) 2977
BspMAI CTGCAG 2 cut(s) 784, 1327
BspMI ACCTGC 2 cut(s) 1437, 2737
BspPI GGATC 8 cut(s) 289, 604, 662, 667, 996, 1254, 1398, 3018
BspQI GCTCTTC 1 cut(s) 369
BspT104I TTCGAA 1 cut(s) 2900
BspTI CTTAAG 5 cut(s) 371, 502, 1598, 2570, 2759
BspTNI GGTCTC 1 cut(s) 965
BsrDI GCAATG 3 cut(s) 101, 1417, 2145
BsrFI RCCGGY 1 cut(s) 3095
BsrI ACTGG 6 cut(s) 241, 1105, 1929, 2151, 2193, 2307
BssAI RCCGGY 1 cut(s) 3095
BssECI CCNNGG 5 cut(s) 123, 336, 842, 1416, 2376
BssSI CACGAG 2 cut(s) 648, 2892
BssT1I CCWWGG 1 cut(s) 842
Bst2BI CACGAG 2 cut(s) 648, 2892
Bst2UI CCWGG 2 cut(s) 2216, 2378
Bst6I CTCTTC 4 cut(s) 369, 453, 2165, 2286
BstAFI CTTAAG 5 cut(s) 371, 502, 1598, 2570, 2759
BstAPI GCANNNNNTGC 2 cut(s) 779, 2829
BstBI TTCGAA 1 cut(s) 2900
BstC8I GCNNGC 4 cut(s) 423, 1518, 1773, 2517
BstDSI CCRYGG 2 cut(s) 123, 1416
BstF5I GGATG 6 cut(s) 133, 1084, 1372, 2001, 2722, 3306
BstFNI CGCG 1 cut(s) 16
BstHHI GCGC 3 cut(s) 16, 2791, 2834
BstMAI GTCTC 9 cut(s) 165, 277, 693, 858, 965, 1432, 1476, 3124, 3257
BstMWI GCNNNNNNNGC 9 cut(s) 779, 917, 1381, 1501, 1510, 2006, 2051, 2135, 2829
BstNI CCWGG 2 cut(s) 2216, 2378
BstNSI RCATGY 5 cut(s) 404, 1812, 1974, 2705, 2916
BstSCI CCNGG 2 cut(s) 2214, 2376
BstSFI CTRYAG 4 cut(s) 162, 780, 1323, 1474
BstSLI GKGCMC 1 cut(s) 2521
BstUI CGCG 1 cut(s) 16
BstV1I GCAGC 5 cut(s) 1309, 1419, 1525, 2338, 2953
BstV2I GAAGAC 3 cut(s) 43, 855, 2086
BstX2I RGATCY 5 cut(s) 667, 988, 1246, 1390, 3010
BstXI CCANNNNNNTGG 1 cut(s) 2287
BstYI RGATCY 5 cut(s) 667, 988, 1246, 1390, 3010
BsuRI GGCC 3 cut(s) 920, 1964, 2099
BtgI CCRYGG 2 cut(s) 123, 1416
BtgZI GCGATG 1 cut(s) 31
BtsCI GGATG 6 cut(s) 133, 1084, 1372, 2001, 2722, 3306
BtsI GCAGTG 2 cut(s) 2018, 3138
BtsIMutI CAGTG 6 cut(s) 516, 631, 2018, 2158, 3138, 3163
BveI ACCTGC 2 cut(s) 1437, 2737
Cac8I GCNNGC 4 cut(s) 423, 1518, 1773, 2517
CciI TCATGA 1 cut(s) 829
CfoI GCGC 3 cut(s) 16, 2791, 2834
Cfr10I RCCGGY 1 cut(s) 3095
Cfr13I GGNCC 2 cut(s) 1402, 2191
CseI GACGC 2 cut(s) 107, 346
Csp6I GTAC 6 cut(s) 642, 707, 804, 1241, 1609, 3302
CspCI CAANNNNNGTGG 2 cut(s) 547, 582
CviQI GTAC 6 cut(s) 642, 707, 804, 1241, 1609, 3302
DraI TTTAAA 1 cut(s) 2698
Eam1104I CTCTTC 4 cut(s) 369, 453, 2165, 2286
EarI CTCTTC 4 cut(s) 369, 453, 2165, 2286
EciI GGCGGA 1 cut(s) 908
Ecl136II GAGCTC 3 cut(s) 574, 1495, 2212
Eco130I CCWWGG 1 cut(s) 842
Eco24I GRGCYC 3 cut(s) 576, 1497, 2214
Eco31I GGTCTC 1 cut(s) 965
Eco47I GGWCC 2 cut(s) 1402, 2191
Eco53kI GAGCTC 3 cut(s) 574, 1495, 2212
Eco57I CTGAAG 5 cut(s) 849, 1713, 1875, 2048, 3108
Eco88I CYCGRG 3 cut(s) 1433, 1496, 2939
EcoICRI GAGCTC 3 cut(s) 574, 1495, 2212
EcoRI GAATTC 3 cut(s) 376, 1895, 2072
EcoRII CCWGG 2 cut(s) 2214, 2376
EcoT14I CCWWGG 1 cut(s) 842
EcoT22I ATGCAT 1 cut(s) 402
EcoT38I GRGCYC 3 cut(s) 576, 1497, 2214
ErhI CCWWGG 1 cut(s) 842
Esp3I CGTCTC 2 cut(s) 165, 693
FaqI GGGAC 4 cut(s) 110, 1369, 1606, 2958
FauI CCCGC 2 cut(s) 1816, 2508
FblI GTMKAC 1 cut(s) 258
Fnu4HI GCNGC 6 cut(s) 1323, 1408, 1514, 2327, 2615, 2967
FokI GGATG 6 cut(s) 140, 1091, 1379, 1988, 2729, 3293
FriOI GRGCYC 3 cut(s) 576, 1497, 2214
Fsp4HI GCNGC 6 cut(s) 1323, 1408, 1514, 2327, 2615, 2967
FspBI CTAG 6 cut(s) 908, 912, 935, 1571, 2031, 2180
FspI TGCGCA 1 cut(s) 2833
GlaI GCGC 3 cut(s) 15, 2790, 2833
GluI GCNGC 6 cut(s) 1323, 1408, 1514, 2327, 2615, 2967
GsuI CTGGAG 6 cut(s) 1184, 1271, 1406, 1946, 2198, 2999
HaeIII GGCC 3 cut(s) 920, 1964, 2099
HapII CCGG 1 cut(s) 3096
HgaI GACGC 2 cut(s) 107, 346
HhaI GCGC 3 cut(s) 16, 2791, 2834
Hin6I GCGC 3 cut(s) 14, 2789, 2832
HinP1I GCGC 3 cut(s) 14, 2789, 2832
HincII GTYRAC 1 cut(s) 472
HindII GTYRAC 1 cut(s) 472
HindIII AAGCTT 3 cut(s) 487, 1747, 1909
HpaI GTTAAC 1 cut(s) 472
HpaII CCGG 1 cut(s) 3096
HphI GGTGA 8 cut(s) 91, 467, 1130, 1472, 2168, 2392, 2650, 3287
Hpy166II GTNNAC 4 cut(s) 259, 472, 2156, 3215
Hpy8I GTNNAC 4 cut(s) 259, 472, 2156, 3215
Hpy99I CGWCG 2 cut(s) 362, 688
HpyCH4IV ACGT 2 cut(s) 1072, 1100
HpyF10VI GCNNNNNNNGC 9 cut(s) 779, 917, 1381, 1501, 1510, 2006, 2051, 2135, 2829
HpySE526I ACGT 2 cut(s) 1072, 1100
HspAI GCGC 3 cut(s) 14, 2789, 2832
KspAI GTTAAC 1 cut(s) 472
LguI GCTCTTC 1 cut(s) 369
Lsp1109I GCAGC 5 cut(s) 1309, 1419, 1525, 2338, 2953
MaeI CTAG 6 cut(s) 908, 912, 935, 1571, 2031, 2180
MaeII ACGT 2 cut(s) 1072, 1100
MfeI CAATTG 1 cut(s) 534
MflI RGATCY 5 cut(s) 667, 988, 1246, 1390, 3010
MhlI GDGCHC 5 cut(s) 576, 1497, 2214, 2521, 3044
MlyI GAGTC 6 cut(s) 703, 779, 1123, 2076, 2182, 2643
MmeI TCCRAC 3 cut(s) 699, 1320, 2626
Mph1103I ATGCAT 1 cut(s) 402
MroXI GAANNNNTTC 1 cut(s) 837
MspA1I CMGCKG 3 cut(s) 760, 2054, 2801
MspCI CTTAAG 5 cut(s) 371, 502, 1598, 2570, 2759
MspI CCGG 1 cut(s) 3096
MspR9I CCNGG 2 cut(s) 2216, 2378
MunI CAATTG 1 cut(s) 534
Mva1269I GAATGC 1 cut(s) 1619
MvaI CCWGG 2 cut(s) 2216, 2378
MvnI CGCG 1 cut(s) 16
MwoI GCNNNNNNNGC 9 cut(s) 779, 917, 1381, 1501, 1510, 2006, 2051, 2135, 2829
NlaIV GGNNCC 1 cut(s) 2977
NmeAIII GCCGAG 1 cut(s) 361
NmuCI GTSAC 6 cut(s) 79, 695, 1811, 2174, 2638, 3158
NsbI TGCGCA 1 cut(s) 2833
NsiI ATGCAT 1 cut(s) 402
NspI RCATGY 5 cut(s) 404, 1812, 1974, 2705, 2916
NspV TTCGAA 1 cut(s) 2900
PaeR7I CTCGAG 3 cut(s) 1433, 1496, 2939
PagI TCATGA 1 cut(s) 829
PaqCI CACCTGC 1 cut(s) 1437
PciI ACATGT 2 cut(s) 1808, 1970
PciSI GCTCTTC 1 cut(s) 369
PctI GAATGC 1 cut(s) 1619
PdmI GAANNNNTTC 1 cut(s) 837
PfeI GAWTC 6 cut(s) 118, 1028, 1214, 1307, 1733, 2404
PflMI CCANNNNNTGG 1 cut(s) 236
PfoI TCCNGGA 1 cut(s) 2214
PkrI GCNGC 6 cut(s) 1324, 1409, 1515, 2328, 2616, 2968
PleI GAGTC 6 cut(s) 702, 779, 1123, 2076, 2181, 2643
PpsI GAGTC 6 cut(s) 702, 779, 1123, 2076, 2181, 2643
PscI ACATGT 2 cut(s) 1808, 1970
PshBI ATTAAT 1 cut(s) 549
Psp124BI GAGCTC 3 cut(s) 576, 1497, 2214
Psp6I CCWGG 2 cut(s) 2214, 2376
PspGI CCWGG 2 cut(s) 2214, 2376
PspN4I GGNNCC 1 cut(s) 2977
PspPI GGNCC 2 cut(s) 1402, 2191
PspXI VCTCGAGB 1 cut(s) 2939
PsrI GAACNNNNNNTAC 2 cut(s) 796, 828
PstI CTGCAG 2 cut(s) 784, 1327
PsuI RGATCY 5 cut(s) 667, 988, 1246, 1390, 3010
PvuII CAGCTG 1 cut(s) 2054
RsaI GTAC 6 cut(s) 643, 708, 805, 1242, 1610, 3303
RsaNI GTAC 6 cut(s) 642, 707, 804, 1241, 1609, 3302
SacI GAGCTC 3 cut(s) 576, 1497, 2214
SapI GCTCTTC 1 cut(s) 369
SatI GCNGC 6 cut(s) 1323, 1408, 1514, 2327, 2615, 2967
Sau96I GGNCC 2 cut(s) 1402, 2191
ScaI AGTACT 1 cut(s) 805
SchI GAGTC 6 cut(s) 703, 779, 1123, 2076, 2182, 2643
ScrFI CCNGG 2 cut(s) 2216, 2378
SduI GDGCHC 5 cut(s) 576, 1497, 2214, 2521, 3044
SfcI CTRYAG 4 cut(s) 162, 780, 1323, 1474
Sfr274I CTCGAG 3 cut(s) 1433, 1496, 2939
SfuI TTCGAA 1 cut(s) 2900
SinI GGWCC 2 cut(s) 1402, 2191
SlaI CTCGAG 3 cut(s) 1433, 1496, 2939
SspMI CTAG 6 cut(s) 908, 912, 935, 1571, 2031, 2180
SstI GAGCTC 3 cut(s) 576, 1497, 2214
StyD4I CCNGG 2 cut(s) 2214, 2376
StyI CCWWGG 1 cut(s) 842
TaiI ACGT 2 cut(s) 1075, 1103
TatI WGTACW 2 cut(s) 706, 803
TauI GCSGC 1 cut(s) 2617
TfiI GAWTC 6 cut(s) 118, 1028, 1214, 1307, 1733, 2404
TscAI CASTG 6 cut(s) 523, 631, 2025, 2158, 3145, 3163
TseFI GTSAC 6 cut(s) 79, 695, 1811, 2174, 2638, 3158
TseI GCWGC 5 cut(s) 1322, 1407, 1513, 2326, 2966
Tsp45I GTSAC 6 cut(s) 79, 695, 1811, 2174, 2638, 3158
TspGWI ACGGA 5 cut(s) 212, 672, 1141, 1433, 1688
TspRI CASTG 6 cut(s) 523, 631, 2025, 2158, 3145, 3163
Van91I CCANNNNNTGG 1 cut(s) 236
Vha464I CTTAAG 5 cut(s) 371, 502, 1598, 2570, 2759
VpaK11BI GGWCC 2 cut(s) 1402, 2191
VspI ATTAAT 1 cut(s) 549
XceI RCATGY 5 cut(s) 404, 1812, 1974, 2705, 2916
XcmI CCANNNNNNNNNTGG 1 cut(s) 1112
XhoI CTCGAG 3 cut(s) 1433, 1496, 2939
XmiI GTMKAC 1 cut(s) 258
XmnI GAANNNNTTC 1 cut(s) 837
XspI CTAG 6 cut(s) 908, 912, 935, 1571, 2031, 2180
ZrmI AGTACT 1 cut(s) 805
Zsp2I ATGCAT 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.