pycom14g10960

FAS-associated factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0012942
Physical Location & Seq
Forward (+)
13769882 .. 13772254
2373 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1041 bp
ATGGTCGATGCCAACGACAAGTTAGCGTATTTCCAAGCGATCACAGGCCTCGAAGACACCGATTTGTGCGAGGAGATTCTCACCGCCCATGGCTGGGACCTCGAGCTCGCGATCTCCTCCTTCACCACCACTGCGAACCATACGCCTTCGCCGGAAGATCCATCCGATTCCGGAACCACCTCCATAGTCTCCGATTTCGGCGGGGACGGCCTTCGCTCCATTCCCCAATCGTCGCACCAGTCTGGACAGTCGTCAAACGCGGTCGCAGCACCCGGTTTTGCTTGGAGGATCATCACTCTGCCTATTTCGGTTATTTCTGGTAGTCTAGGGTTGATTTCTCGGGCGGTTGGGCTCGGGTTGTGGGCCGCCGGTGGAGTGCTATCGTACTCGGTCGGAATGATTGGACTCCGGGTTGGTCGGGACGGCGAGGCCTCTGCCCAATTACTGTCGGTGCCGGCGGTGGGGAATGAAGCAGTGCAGTTTGTAGCGAGGTTTGAGCAGGATTTTGGGACGAGGAGGCCGAATTTTGTCAGCGAGGGGTTTATGGACGCCCTGCAGAGGTCGAGAAACTCGTTCAAGCTGTTGTTCGTCTACTTGCACTCGCCAGATCATCCTGATACGCCGTCGTTTTGTGAGAGGACTCTGTGCTCTGAGATGTTGGTGGCTTATGTTAATGAGAATTTCGTGTCGTGGGGAGGGAGCATTCGGGCAAGTGAAGGCTTCAAGATGAGTAACAGTCTGAAGGCCTCGAGATATCCCTTCTGTGCCGTCGTAATGGCTGCCACGAACCAGAGGATCTTACTGCTTCAGCAGGTTGAGGGGCCAAAGTCTCCTGAAGAAATGCTCTCAATACTAAACAGAGTGGTTGAAGAAAGTGCTTCTGTTCTTGGCACAGCAAGGCATGAGGCAGAACAAAGGAGAATCAACGTTCGTTTAAGGGAGGAGCAAGATGCTGCTTATAGAGCAGCACTTGAAGCTCGATCAGGCGAGGGAAAACCAGAGGAGGGAAGAGCAAGAACGTCTAGAAAGAGAAGCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UBA_4 UBX

Protein Analysis

347

Amino Acids

37.5

Weight (kDa)

5.4

Isoelectric Point (pI)

50.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_4 PF14555 6 - 43 7.9e-11 UBA-like domain
UBA_8 PF22566 6 - 43 8.6e-07 UBA-like domain
FAF1 PF21021 161 - 236 1.8e-10 Fas-associated factor 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012942)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10790
fragaria_vesca FvH4_5g11270
malus_domestica MD06G1114700.v1.1 MD14G1136100.v1.1
prunus_persica Prupe.5G127400_v2.0.a1
pyrus_communis pycom06g10750 pycom14g10960 pycom14g10970
rosa_chinensis RchiOBHm_Chr7g0186581
rosa_laevigata RLG00000004830
rosa_multiflora Rmu_sc0007722.1_g000020
rosa_rugosa Rorug06G0475200
rosa_samantha Rh7AG065700 Rh7BG081600 Rh7CG082100 Rh7DG083100
rosa_wichuraiana Rw7G006910 Rw7G007130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 802
AccB1I GGYRCC 1 cut(s) 451
AccI GTMKAC 1 cut(s) 591
AccII CGCG 2 cut(s) 110, 260
AccIII TCCGGA 1 cut(s) 170
AciI CCGC 6 cut(s) 84, 201, 260, 344, 366, 458
AclI AACGTT 1 cut(s) 927
AclWI GGATC 3 cut(s) 152, 296, 803
AcsI RAATTY 2 cut(s) 523, 679
AcuI CTGAAG 3 cut(s) 761, 791, 855
AcyI GRCGYC 1 cut(s) 549
AfaI GTAC 1 cut(s) 386
AfiI CCNNNNNNNGG 5 cut(s) 93, 94, 461, 558, 1004
AgsI TTSAA 4 cut(s) 577, 724, 869, 974
AluBI AGCT 4 cut(s) 106, 580, 977, 1035
AluI AGCT 4 cut(s) 106, 580, 977, 1035
Alw21I GWGCWC 2 cut(s) 108, 650
Alw26I GTCTC 2 cut(s) 193, 834
AlwI GGATC 3 cut(s) 152, 296, 803
Ama87I CYCGRG 4 cut(s) 101, 339, 353, 748
Aor13HI TCCGGA 1 cut(s) 170
AoxI GGCC 7 cut(s) 46, 208, 363, 429, 518, 744, 821
ApeKI GCWGC 5 cut(s) 266, 779, 953, 965, 1035
ApoI RAATTY 2 cut(s) 523, 679
AspS9I GGNCC 3 cut(s) 97, 363, 821
AsuC2I CCSGG 2 cut(s) 273, 410
AsuHPI GGTGA 2 cut(s) 73, 115
AvaI CYCGRG 4 cut(s) 101, 339, 353, 748
AvaII GGWCC 1 cut(s) 97
BanI GGYRCC 1 cut(s) 451
BanII GRGCYC 2 cut(s) 108, 354
BbsI GAAGAC 1 cut(s) 60
Bbv12I GWGCWC 2 cut(s) 108, 650
BbvI GCAGC 5 cut(s) 278, 766, 940, 977, 1022
BccI CCATC 1 cut(s) 169
BceAI ACGGC 4 cut(s) 223, 439, 607, 752
BcgI CGANNNNNNTGC 2 cut(s) 416, 450
BcnI CCSGG 2 cut(s) 273, 410
BcoDI GTCTC 2 cut(s) 193, 834
BfaI CTAG 2 cut(s) 326, 1023
BfmI CTRYAG 1 cut(s) 554
BfuAI ACCTGC 1 cut(s) 802
BisI GCNGC 6 cut(s) 267, 366, 780, 954, 966, 1036
BlsI GCNGC 6 cut(s) 268, 367, 781, 955, 967, 1037
Bme1390I CCNGG 2 cut(s) 273, 410
Bme18I GGWCC 1 cut(s) 97
BmeT110I CYCGRG 4 cut(s) 101, 339, 353, 748
BmgT120I GGNCC 3 cut(s) 97, 363, 821
BmiI GGNNCC 4 cut(s) 98, 175, 453, 822
BmrFI CCNGG 2 cut(s) 273, 410
BmsI GCATC 1 cut(s) 940
BoxI GACNNNNGTC 1 cut(s) 250
BpiI GAAGAC 1 cut(s) 60
BpuMI CCSGG 2 cut(s) 273, 410
BsaHI GRCGYC 1 cut(s) 549
BsaJI CCNNGG 1 cut(s) 88
BsaWI WCCGGW 1 cut(s) 170
Bsc4I CCNNNNNNNGG 5 cut(s) 93, 94, 461, 558, 1004
Bse118I RCCGGY 2 cut(s) 368, 454
Bse1I ACTGG 1 cut(s) 238
BseAI TCCGGA 1 cut(s) 170
BseDI CCNNGG 1 cut(s) 88
BseGI GGATG 2 cut(s) 161, 610
BseLI CCNNNNNNNGG 5 cut(s) 93, 94, 461, 558, 1004
BseMII CTCAG 1 cut(s) 642
BseNI ACTGG 1 cut(s) 238
BseRI GAGGAG 5 cut(s) 86, 106, 529, 956, 1016
BseXI GCAGC 5 cut(s) 278, 766, 940, 977, 1022
BseYI CCCAGC 1 cut(s) 93
BsgI GTGCAG 1 cut(s) 497
Bsh1236I CGCG 2 cut(s) 110, 260
Bsh1285I CGRYCG 2 cut(s) 264, 393
BshFI GGCC 7 cut(s) 48, 210, 365, 431, 520, 746, 823
BshNI GGYRCC 1 cut(s) 451
BsiEI CGRYCG 2 cut(s) 264, 393
BsiHKAI GWGCWC 2 cut(s) 108, 650
BsiHKCI CYCGRG 4 cut(s) 101, 339, 353, 748
BsiSI CCGG 6 cut(s) 152, 171, 273, 369, 409, 455
BslFI GGGAC 4 cut(s) 110, 218, 434, 523
BslI CCNNNNNNNGG 5 cut(s) 93, 94, 461, 558, 1004
BsmAI GTCTC 2 cut(s) 193, 834
BsmFI GGGAC 4 cut(s) 110, 218, 434, 523
BsmI GAATGC 1 cut(s) 702
BsnI GGCC 7 cut(s) 48, 210, 365, 431, 520, 746, 823
BsoBI CYCGRG 4 cut(s) 101, 339, 353, 748
Bsp1286I GDGCHC 3 cut(s) 108, 354, 650
Bsp13I TCCGGA 1 cut(s) 170
Bsp143I GATC 7 cut(s) 39, 111, 157, 288, 607, 795, 980
Bsp19I CCATGG 1 cut(s) 88
Bsp68I TCGCGA 1 cut(s) 110
BspACI CCGC 6 cut(s) 84, 201, 260, 344, 366, 458
BspANI GGCC 7 cut(s) 48, 210, 365, 431, 520, 746, 823
BspCNI CTCAG 1 cut(s) 643
BspEI TCCGGA 1 cut(s) 170
BspFNI CGCG 2 cut(s) 110, 260
BspLI GGNNCC 4 cut(s) 98, 175, 453, 822
BspMAI CTGCAG 1 cut(s) 558
BspMI ACCTGC 1 cut(s) 802
BspPI GGATC 3 cut(s) 152, 296, 803
BspQI GCTCTTC 1 cut(s) 1003
BspT107I GGYRCC 1 cut(s) 451
BsrFI RCCGGY 2 cut(s) 368, 454
BsrI ACTGG 1 cut(s) 238
BssAI RCCGGY 2 cut(s) 368, 454
BssECI CCNNGG 1 cut(s) 88
BssMI GATC 7 cut(s) 39, 111, 157, 288, 607, 795, 980
BssNI GRCGYC 1 cut(s) 549
BssT1I CCWWGG 1 cut(s) 88
Bst4CI ACNGT 3 cut(s) 249, 447, 737
Bst6I CTCTTC 1 cut(s) 1003
BstACI GRCGYC 1 cut(s) 549
BstC8I GCNNGC 2 cut(s) 108, 456
BstDEI CTNAG 1 cut(s) 651
BstDSI CCRYGG 1 cut(s) 88
BstF5I GGATG 2 cut(s) 161, 610
BstFNI CGCG 2 cut(s) 110, 260
BstKTI GATC 7 cut(s) 42, 114, 160, 291, 610, 798, 983
BstMAI GTCTC 2 cut(s) 193, 834
BstMBI GATC 7 cut(s) 39, 111, 157, 288, 607, 795, 980
BstMCI CGRYCG 2 cut(s) 264, 393
BstMWI GCNNNNNNNGC 4 cut(s) 207, 266, 962, 974
BstPAI GACNNNNGTC 1 cut(s) 250
BstSCI CCNGG 2 cut(s) 271, 408
BstSFI CTRYAG 1 cut(s) 554
BstUI CGCG 2 cut(s) 110, 260
BstV1I GCAGC 5 cut(s) 278, 766, 940, 977, 1022
BstV2I GAAGAC 1 cut(s) 60
BstX2I RGATCY 2 cut(s) 157, 795
BstYI RGATCY 2 cut(s) 157, 795
BsuRI GGCC 7 cut(s) 48, 210, 365, 431, 520, 746, 823
BtgI CCRYGG 1 cut(s) 88
BtsCI GGATG 2 cut(s) 161, 610
BtsI GCAGTG 2 cut(s) 129, 480
BtsIMutI CAGTG 2 cut(s) 129, 480
BtuMI TCGCGA 1 cut(s) 110
BveI ACCTGC 1 cut(s) 802
Cac8I GCNNGC 2 cut(s) 108, 456
Cfr10I RCCGGY 2 cut(s) 368, 454
Cfr13I GGNCC 3 cut(s) 97, 363, 821
CseI GACGC 1 cut(s) 557
Csp6I GTAC 1 cut(s) 385
CviAII CATG 2 cut(s) 89, 902
CviQI GTAC 1 cut(s) 385
DdeI CTNAG 1 cut(s) 651
DpnI GATC 7 cut(s) 41, 113, 159, 290, 609, 797, 982
DpnII GATC 7 cut(s) 39, 111, 157, 288, 607, 795, 980
Eam1104I CTCTTC 1 cut(s) 1003
EarI CTCTTC 1 cut(s) 1003
Ecl136II GAGCTC 1 cut(s) 106
Eco130I CCWWGG 1 cut(s) 88
Eco147I AGGCCT 3 cut(s) 48, 431, 746
Eco24I GRGCYC 2 cut(s) 108, 354
Eco32I GATATC 1 cut(s) 755
Eco47I GGWCC 1 cut(s) 97
Eco53kI GAGCTC 1 cut(s) 106
Eco57I CTGAAG 3 cut(s) 761, 791, 855
Eco88I CYCGRG 4 cut(s) 101, 339, 353, 748
EcoICRI GAGCTC 1 cut(s) 106
EcoO109I RGGNCCY 1 cut(s) 97
EcoRV GATATC 1 cut(s) 755
EcoT14I CCWWGG 1 cut(s) 88
EcoT38I GRGCYC 2 cut(s) 108, 354
ErhI CCWWGG 1 cut(s) 88
FaeI CATG 2 cut(s) 92, 905
FaiI YATR 7 cut(s) 90, 141, 185, 545, 669, 903, 960
FaqI GGGAC 4 cut(s) 110, 218, 434, 523
FatI CATG 2 cut(s) 88, 901
FauI CCCGC 1 cut(s) 194
FblI GTMKAC 1 cut(s) 591
Fnu4HI GCNGC 6 cut(s) 267, 366, 780, 954, 966, 1036
FokI GGATG 2 cut(s) 148, 597
FriOI GRGCYC 2 cut(s) 108, 354
Fsp4HI GCNGC 6 cut(s) 267, 366, 780, 954, 966, 1036
FspBI CTAG 2 cut(s) 326, 1023
GluI GCNGC 6 cut(s) 267, 366, 780, 954, 966, 1036
GsaI CCCAGC 1 cut(s) 97
HaeIII GGCC 7 cut(s) 48, 210, 365, 431, 520, 746, 823
HapII CCGG 6 cut(s) 152, 171, 273, 369, 409, 455
HgaI GACGC 1 cut(s) 557
Hin1I GRCGYC 1 cut(s) 549
Hin1II CATG 2 cut(s) 92, 905
HinfI GANTC 5 cut(s) 76, 167, 405, 640, 921
HpaII CCGG 6 cut(s) 152, 171, 273, 369, 409, 455
HphI GGTGA 2 cut(s) 73, 115
Hpy166II GTNNAC 1 cut(s) 592
Hpy188I TCNGA 5 cut(s) 166, 193, 395, 652, 741
Hpy8I GTNNAC 1 cut(s) 592
Hpy99I CGWCG 3 cut(s) 235, 628, 773
HpyAV CCTTC 6 cut(s) 130, 156, 221, 710, 736, 769
HpyCH4III ACNGT 3 cut(s) 249, 447, 737
HpyCH4IV ACGT 2 cut(s) 927, 1019
HpyCH4V TGCA 3 cut(s) 478, 556, 598
HpyF10VI GCNNNNNNNGC 4 cut(s) 207, 266, 962, 974
HpyF3I CTNAG 1 cut(s) 651
HpySE526I ACGT 2 cut(s) 927, 1019
Hsp92I GRCGYC 1 cut(s) 549
Hsp92II CATG 2 cut(s) 92, 905
Kpn2I TCCGGA 1 cut(s) 170
KroI GCCGGC 1 cut(s) 454
KroNI GCCGGC 1 cut(s) 456
Kzo9I GATC 7 cut(s) 39, 111, 157, 288, 607, 795, 980
LguI GCTCTTC 1 cut(s) 1003
LmnI GCTCC 3 cut(s) 221, 699, 943
Lsp1109I GCAGC 5 cut(s) 278, 766, 940, 977, 1022
LweI GCATC 1 cut(s) 940
MaeI CTAG 2 cut(s) 326, 1023
MaeII ACGT 2 cut(s) 927, 1019
MaeIII GTNAC 1 cut(s) 731
MalI GATC 7 cut(s) 41, 113, 159, 290, 609, 797, 982
MboI GATC 7 cut(s) 39, 111, 157, 288, 607, 795, 980
MboII GAAGA 5 cut(s) 65, 167, 848, 881, 1020
MflI RGATCY 2 cut(s) 157, 795
MhlI GDGCHC 3 cut(s) 108, 354, 650
MluCI AATT 3 cut(s) 440, 523, 679
MlyI GAGTC 2 cut(s) 399, 634
MmeI TCCRAC 1 cut(s) 373
MroI TCCGGA 1 cut(s) 170
MroNI GCCGGC 1 cut(s) 454
MseI TTAA 2 cut(s) 672, 935
MspI CCGG 6 cut(s) 152, 171, 273, 369, 409, 455
MspR9I CCNGG 2 cut(s) 273, 410
Mva1269I GAATGC 1 cut(s) 702
MvnI CGCG 2 cut(s) 110, 260
MwoI GCNNNNNNNGC 4 cut(s) 207, 266, 962, 974
NaeI GCCGGC 1 cut(s) 456
NciI CCSGG 2 cut(s) 273, 410
NcoI CCATGG 1 cut(s) 88
NdeII GATC 7 cut(s) 39, 111, 157, 288, 607, 795, 980
NgoMIV GCCGGC 1 cut(s) 454
NlaIII CATG 2 cut(s) 92, 905
NlaIV GGNNCC 4 cut(s) 98, 175, 453, 822
NruI TCGCGA 1 cut(s) 110
PaeR7I CTCGAG 2 cut(s) 101, 748
PceI AGGCCT 3 cut(s) 48, 431, 746
PciSI GCTCTTC 1 cut(s) 1003
PcsI WCGNNNNNNNCGW 4 cut(s) 12, 57, 518, 569
PctI GAATGC 1 cut(s) 702
PdiI GCCGGC 1 cut(s) 456
PfeI GAWTC 3 cut(s) 76, 167, 921
PkrI GCNGC 6 cut(s) 268, 367, 781, 955, 967, 1037
PleI GAGTC 2 cut(s) 399, 634
PpsI GAGTC 2 cut(s) 399, 634
PpuMI RGGWCCY 1 cut(s) 97
PshAI GACNNNNGTC 1 cut(s) 250
Psp124BI GAGCTC 1 cut(s) 108
Psp1406I AACGTT 1 cut(s) 927
Psp5II RGGWCCY 1 cut(s) 97
PspFI CCCAGC 1 cut(s) 93
PspN4I GGNNCC 4 cut(s) 98, 175, 453, 822
PspPI GGNCC 3 cut(s) 97, 363, 821
PspPPI RGGWCCY 1 cut(s) 97
PspXI VCTCGAGB 1 cut(s) 101
PstI CTGCAG 1 cut(s) 558
PsuI RGATCY 2 cut(s) 157, 795
RruI TCGCGA 1 cut(s) 110
RsaI GTAC 1 cut(s) 386
RsaNI GTAC 1 cut(s) 385
SacI GAGCTC 1 cut(s) 108
SapI GCTCTTC 1 cut(s) 1003
SaqAI TTAA 2 cut(s) 672, 935
SatI GCNGC 6 cut(s) 267, 366, 780, 954, 966, 1036
Sau3AI GATC 7 cut(s) 39, 111, 157, 288, 607, 795, 980
Sau96I GGNCC 3 cut(s) 97, 363, 821
SchI GAGTC 2 cut(s) 399, 634
ScrFI CCNGG 2 cut(s) 273, 410
SduI GDGCHC 3 cut(s) 108, 354, 650
SfaNI GCATC 1 cut(s) 940
SfcI CTRYAG 1 cut(s) 554
Sfr274I CTCGAG 2 cut(s) 101, 748
SgrAI CRCCGGYG 1 cut(s) 368
SinI GGWCC 1 cut(s) 97
SlaI CTCGAG 2 cut(s) 101, 748
SmlI CTYRAG 2 cut(s) 101, 748
SmoI CTYRAG 2 cut(s) 101, 748
Sse9I AATT 3 cut(s) 440, 523, 679
SseBI AGGCCT 3 cut(s) 48, 431, 746
SsiI CCGC 6 cut(s) 84, 201, 260, 344, 366, 458
SspMI CTAG 2 cut(s) 326, 1023
SstI GAGCTC 1 cut(s) 108
StuI AGGCCT 3 cut(s) 48, 431, 746
StyD4I CCNGG 2 cut(s) 271, 408
StyI CCWWGG 1 cut(s) 88
TaaI ACNGT 3 cut(s) 249, 447, 737
TaiI ACGT 2 cut(s) 930, 1022
TaqI TCGA 6 cut(s) 6, 51, 102, 563, 749, 979
TaqII GACCGA 1 cut(s) 379
TasI AATT 3 cut(s) 440, 523, 679
TauI GCSGC 1 cut(s) 368
TfiI GAWTC 3 cut(s) 76, 167, 921
Tru1I TTAA 2 cut(s) 672, 935
Tru9I TTAA 2 cut(s) 672, 935
TscAI CASTG 2 cut(s) 136, 480
TseI GCWGC 5 cut(s) 266, 779, 953, 965, 1035
TspDTI ATGAA 1 cut(s) 483
TspRI CASTG 2 cut(s) 136, 480
VpaK11BI GGWCC 1 cut(s) 97
XapI RAATTY 2 cut(s) 523, 679
XbaI TCTAGA 1 cut(s) 1022
XhoI CTCGAG 2 cut(s) 101, 748
XmiI GTMKAC 1 cut(s) 591
XspI CTAG 2 cut(s) 326, 1023
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.