pycom14g12540

Mitochondrial import inner membrane translocase subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0015070
Physical Location & Seq
Forward (+)
15536097 .. 15537206
1110 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 237 bp
ATGGATCCTTCAGCAATGAACAACCCTGAACTGCTTAATTTCATCAACCAAGAGAAGGAAAGAGCAATGGTGAACGAGATGGTGGGAAAGCTTACAAATGTATGTTGGGACAAGTGCATCACCAGTACCCCTGGGAGCAAGTTCAGTTCCAGTGAGTCCGCTTGCCTGTCGAACTGTGCCCGCCGGTATCTTGATATGAGTATGATTATTATGAAGCGTTTTCAGAGTATGCAATGA

Protein Analysis

79

Amino Acids

8.89

Weight (kDa)

7.64

Isoelectric Point (pI)

49.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-Tim10_DDP PF02953 13 - 76 2.1e-26 Tim10/DDP family zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015070)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50810
fragaria_vesca FvH4_5g08990
malus_domestica MD06G1135800.v1.1 MD14G1151000.v1.1
prunus_persica Prupe.5G146200_v2.0.a1
pyrus_communis pycom06g12700 pycom14g12540
rosa_chinensis RchiOBHm_Chr7g0189291
rosa_multiflora Rmu_sc0004318.1_g000016
rosa_roxburghii Rroxscaffold_3G00265550
rosa_rugosa Rorug06G0496900
rosa_samantha Rh7AG102000 Rh7BG104600 Rh7CG105900 Rh7DG103700
rosa_wichuraiana Rw7G008810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 159, 181
AclWI GGATC 1 cut(s) 12
AfaI GTAC 1 cut(s) 127
AfiI CCNNNNNNNGG 1 cut(s) 55
AjnI CCWGG 1 cut(s) 130
AluBI AGCT 1 cut(s) 91
AluI AGCT 1 cut(s) 91
AlwI GGATC 1 cut(s) 12
AsuHPI GGTGA 2 cut(s) 82, 112
BaeGI GKGCMC 1 cut(s) 181
BamHI GGATCC 1 cut(s) 4
BccI CCATC 1 cut(s) 73
BciT130I CCWGG 1 cut(s) 132
Bme1390I CCNGG 1 cut(s) 132
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 132
BmsI GCATC 1 cut(s) 126
BsaJI CCNNGG 2 cut(s) 130, 131
Bsc4I CCNNNNNNNGG 1 cut(s) 55
Bse118I RCCGGY 1 cut(s) 183
Bse1I ACTGG 2 cut(s) 123, 150
Bse3DI GCAATG 2 cut(s) 21, 72
BseBI CCWGG 1 cut(s) 132
BseDI CCNNGG 2 cut(s) 130, 131
BseLI CCNNNNNNNGG 1 cut(s) 55
BseMI GCAATG 2 cut(s) 21, 72
BseNI ACTGG 2 cut(s) 123, 150
BseSI GKGCMC 1 cut(s) 181
BsiSI CCGG 1 cut(s) 184
BslFI GGGAC 1 cut(s) 122
BslI CCNNNNNNNGG 1 cut(s) 55
BsmFI GGGAC 1 cut(s) 122
Bsp1286I GDGCHC 1 cut(s) 181
Bsp143I GATC 1 cut(s) 4
BspACI CCGC 2 cut(s) 159, 181
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 1 cut(s) 12
BsrDI GCAATG 2 cut(s) 21, 72
BsrFI RCCGGY 1 cut(s) 183
BsrI ACTGG 2 cut(s) 123, 150
BssAI RCCGGY 1 cut(s) 183
BssECI CCNNGG 2 cut(s) 130, 131
BssMI GATC 1 cut(s) 4
Bst2UI CCWGG 1 cut(s) 132
Bst4CI ACNGT 1 cut(s) 176
BstC8I GCNNGC 2 cut(s) 163, 181
BstKTI GATC 1 cut(s) 7
BstMBI GATC 1 cut(s) 4
BstNI CCWGG 1 cut(s) 132
BstSCI CCNGG 1 cut(s) 130
BstSLI GKGCMC 1 cut(s) 181
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BtsIMutI CAGTG 1 cut(s) 157
Cac8I GCNNGC 2 cut(s) 163, 181
Cfr10I RCCGGY 1 cut(s) 183
Csp6I GTAC 1 cut(s) 126
CviJI RGCY 1 cut(s) 91
CviKI_1 RGCY 1 cut(s) 91
CviQI GTAC 1 cut(s) 126
DpnI GATC 1 cut(s) 6
DpnII GATC 1 cut(s) 4
EcoRII CCWGG 1 cut(s) 130
FaiI YATR 5 cut(s) 103, 197, 203, 212, 230
FaqI GGGAC 1 cut(s) 122
FauI CCCGC 1 cut(s) 188
HapII CCGG 1 cut(s) 184
HindIII AAGCTT 1 cut(s) 89
HinfI GANTC 1 cut(s) 155
HpaII CCGG 1 cut(s) 184
HphI GGTGA 2 cut(s) 82, 112
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 1 cut(s) 225
Hpy188III TCNNGA 1 cut(s) 191
Hpy8I GTNNAC 1 cut(s) 73
HpyAV CCTTC 2 cut(s) 18, 49
HpyCH4III ACNGT 1 cut(s) 176
HpyCH4V TGCA 2 cut(s) 117, 232
Kzo9I GATC 1 cut(s) 4
LmnI GCTCC 1 cut(s) 135
LpnPI CCDG 7 cut(s) 39, 117, 136, 144, 163, 179, 197
LweI GCATC 1 cut(s) 126
MalI GATC 1 cut(s) 6
MboI GATC 1 cut(s) 4
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 1 cut(s) 181
MluCI AATT 1 cut(s) 37
MlyI GAGTC 1 cut(s) 164
MseI TTAA 1 cut(s) 36
MspI CCGG 1 cut(s) 184
MspR9I CCNGG 1 cut(s) 132
MvaI CCWGG 1 cut(s) 132
NdeII GATC 1 cut(s) 4
NlaIV GGNNCC 1 cut(s) 6
PasI CCCWGGG 1 cut(s) 131
PleI GAGTC 1 cut(s) 163
PpsI GAGTC 1 cut(s) 163
Psp6I CCWGG 1 cut(s) 130
PspGI CCWGG 1 cut(s) 130
PspN4I GGNNCC 1 cut(s) 6
PsuI RGATCY 1 cut(s) 4
RsaI GTAC 1 cut(s) 127
RsaNI GTAC 1 cut(s) 126
SaqAI TTAA 1 cut(s) 36
Sau3AI GATC 1 cut(s) 4
SchI GAGTC 1 cut(s) 164
ScrFI CCNGG 1 cut(s) 132
SduI GDGCHC 1 cut(s) 181
SetI ASST 1 cut(s) 93
SfaNI GCATC 1 cut(s) 126
Sse9I AATT 1 cut(s) 37
SsiI CCGC 2 cut(s) 159, 181
StyD4I CCNGG 1 cut(s) 130
TaaI ACNGT 1 cut(s) 176
TaqI TCGA 1 cut(s) 170
TasI AATT 1 cut(s) 37
Tru1I TTAA 1 cut(s) 36
Tru9I TTAA 1 cut(s) 36
TscAI CASTG 1 cut(s) 157
TspDTI ATGAA 3 cut(s) 31, 32, 227
TspRI CASTG 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.