pycom14g12930

Protein of unknown function (DUF3339)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0019129
Physical Location & Seq
Reverse (-)
15958270 .. 15958794
525 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 441 bp
ATGGGATCACGGCCGTCGAAATCCAACAGTCAAGATCCTGACTCGTCCATTTTCACGAGAAAAACACATGGTGCCTGCCAGTGCATTTATGTTCCCACCCTCAAATTTCTTCAAAAACTTCCAAAACCCATCCACCTTCCTCTCAGATCTTCGAAACCCATCTTTTACTTTATCCTTCTTCTCCTCGTCGTTGGTCTGGTTTTGCAGAGGAGAGCAGGGGCAAAAATGGCAGACTGGGGTCCGGTGGTGATAGCGGTGATGCTGTTTGTACTATTGACACCCGGGCTTTTGTTTCAGATACCCGGACATGGGAGGTTGGTGGAGTTCGGGAACATGGAGACCAGCGGCGCTTCCATTCTGGTACACGCCGTCATCTACTTTGGCCTCATCACCATCTTCCTTATTGCCATTGGAGTTCACATCTATACTGATGCTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3339

Protein Analysis

147

Amino Acids

16.05

Weight (kDa)

9.64

Isoelectric Point (pI)

42.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3339 PF11820 76 - 142 6.5e-35 Protein of unknown function (DUF3339)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019129)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48660 AT5G63500
malus_domestica MD06G1140200.v1.1
prunus_persica Prupe.5G149500_v2.0.a1
pyrus_communis pycom06g13050 pycom14g12930
rosa_rugosa Rorug06G0500800
rosa_wichuraiana Rw7G009180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 71
AciI CCGC 2 cut(s) 254, 345
AclWI GGATC 2 cut(s) 13, 29
AcoI YGGCCR 1 cut(s) 11
AcsI RAATTY 1 cut(s) 104
AdeI CACNNNGTG 1 cut(s) 71
AfaI GTAC 2 cut(s) 270, 363
AfiI CCNNNNNNNGG 2 cut(s) 308, 309
AgsI TTSAA 1 cut(s) 113
Alw26I GTCTC 1 cut(s) 332
AlwI GGATC 2 cut(s) 13, 29
Ama87I CYCGRG 1 cut(s) 281
AoxI GGCC 2 cut(s) 11, 382
ApoI RAATTY 1 cut(s) 104
AspLEI GCGC 1 cut(s) 350
AspS9I GGNCC 1 cut(s) 239
AsuC2I CCSGG 3 cut(s) 282, 283, 303
AsuHPI GGTGA 3 cut(s) 259, 268, 382
AsuII TTCGAA 1 cut(s) 152
AvaI CYCGRG 1 cut(s) 281
AvaII GGWCC 1 cut(s) 239
BanI GGYRCC 1 cut(s) 71
BauI CACGAG 1 cut(s) 55
BccI CCATC 3 cut(s) 137, 167, 401
BceAI ACGGC 2 cut(s) 26, 353
BcnI CCSGG 3 cut(s) 282, 283, 303
BcoDI GTCTC 1 cut(s) 332
BfoI RGCGCY 1 cut(s) 351
BglII AGATCT 1 cut(s) 146
BisI GCNGC 1 cut(s) 346
BlsI GCNGC 1 cut(s) 347
Bme1390I CCNGG 3 cut(s) 282, 283, 303
Bme18I GGWCC 1 cut(s) 239
BmeT110I CYCGRG 1 cut(s) 281
BmgT120I GGNCC 1 cut(s) 239
BmiI GGNNCC 2 cut(s) 73, 240
BmrFI CCNGG 3 cut(s) 282, 283, 303
BmrI ACTGGG 1 cut(s) 244
BmsI GCATC 2 cut(s) 249, 421
BmuI ACTGGG 1 cut(s) 244
BoxI GACNNNNGTC 1 cut(s) 237
Bpu14I TTCGAA 1 cut(s) 152
BpuMI CCSGG 3 cut(s) 282, 283, 303
BsaI GGTCTC 1 cut(s) 332
BsaJI CCNNGG 1 cut(s) 281
BsaWI WCCGGW 1 cut(s) 241
Bsc4I CCNNNNNNNGG 2 cut(s) 308, 309
Bse1I ACTGG 2 cut(s) 79, 239
BseDI CCNNGG 1 cut(s) 281
BseGI GGATG 1 cut(s) 129
BseLI CCNNNNNNNGG 2 cut(s) 308, 309
BseMII CTCAG 1 cut(s) 157
BseNI ACTGG 2 cut(s) 79, 239
BseRI GAGGAG 2 cut(s) 173, 223
BseX3I CGGCCG 1 cut(s) 11
Bsh1285I CGRYCG 1 cut(s) 14
BshFI GGCC 2 cut(s) 13, 384
BshNI GGYRCC 1 cut(s) 71
BsiEI CGRYCG 1 cut(s) 14
BsiHKCI CYCGRG 1 cut(s) 281
BsiSI CCGG 3 cut(s) 242, 282, 303
BslI CCNNNNNNNGG 2 cut(s) 308, 309
BsmAI GTCTC 1 cut(s) 332
BsnI GGCC 2 cut(s) 13, 384
Bso31I GGTCTC 1 cut(s) 332
BsoBI CYCGRG 1 cut(s) 281
Bsp119I TTCGAA 1 cut(s) 152
Bsp143I GATC 3 cut(s) 5, 34, 146
BspACI CCGC 2 cut(s) 254, 345
BspANI GGCC 2 cut(s) 13, 384
BspCNI CTCAG 1 cut(s) 156
BspLI GGNNCC 2 cut(s) 73, 240
BspPI GGATC 2 cut(s) 13, 29
BspT104I TTCGAA 1 cut(s) 152
BspT107I GGYRCC 1 cut(s) 71
BspTNI GGTCTC 1 cut(s) 332
BsrI ACTGG 2 cut(s) 79, 239
BssECI CCNNGG 1 cut(s) 281
BssMI GATC 3 cut(s) 5, 34, 146
BssSI CACGAG 1 cut(s) 55
Bst2BI CACGAG 1 cut(s) 55
Bst4CI ACNGT 1 cut(s) 29
BstBI TTCGAA 1 cut(s) 152
BstC8I GCNNGC 1 cut(s) 76
BstDEI CTNAG 1 cut(s) 143
BstF5I GGATG 1 cut(s) 129
BstH2I RGCGCY 1 cut(s) 351
BstHHI GCGC 1 cut(s) 350
BstKTI GATC 3 cut(s) 8, 37, 149
BstMAI GTCTC 1 cut(s) 332
BstMBI GATC 3 cut(s) 5, 34, 146
BstMCI CGRYCG 1 cut(s) 14
BstMWI GCNNNNNNNGC 1 cut(s) 227
BstPAI GACNNNNGTC 1 cut(s) 237
BstSCI CCNGG 3 cut(s) 280, 281, 301
BstX2I RGATCY 2 cut(s) 34, 146
BstYI RGATCY 2 cut(s) 34, 146
BstZI CGGCCG 1 cut(s) 11
BsuRI GGCC 2 cut(s) 13, 384
BtsCI GGATG 1 cut(s) 129
BtsIMutI CAGTG 1 cut(s) 86
Cac8I GCNNGC 1 cut(s) 76
CfoI GCGC 1 cut(s) 350
Cfr13I GGNCC 1 cut(s) 239
Cfr9I CCCGGG 1 cut(s) 281
Csp6I GTAC 2 cut(s) 269, 362
CviAII CATG 3 cut(s) 68, 308, 334
CviJI RGCY 3 cut(s) 13, 286, 384
CviKI_1 RGCY 3 cut(s) 13, 286, 384
CviQI GTAC 2 cut(s) 269, 362
DdeI CTNAG 1 cut(s) 143
DpnI GATC 3 cut(s) 7, 36, 148
DpnII GATC 3 cut(s) 5, 34, 146
DraIII CACNNNGTG 1 cut(s) 71
EaeI YGGCCR 1 cut(s) 11
EagI CGGCCG 1 cut(s) 11
EclXI CGGCCG 1 cut(s) 11
Eco31I GGTCTC 1 cut(s) 332
Eco47I GGWCC 1 cut(s) 239
Eco52I CGGCCG 1 cut(s) 11
Eco88I CYCGRG 1 cut(s) 281
FaeI CATG 3 cut(s) 71, 311, 337
FaiI YATR 5 cut(s) 69, 90, 309, 335, 426
FatI CATG 3 cut(s) 67, 307, 333
Fnu4HI GCNGC 1 cut(s) 346
FokI GGATG 1 cut(s) 116
Fsp4HI GCNGC 1 cut(s) 346
GlaI GCGC 1 cut(s) 349
GluI GCNGC 1 cut(s) 346
HaeII RGCGCY 1 cut(s) 351
HaeIII GGCC 2 cut(s) 13, 384
HapII CCGG 3 cut(s) 242, 282, 303
HhaI GCGC 1 cut(s) 350
Hin1II CATG 3 cut(s) 71, 311, 337
Hin6I GCGC 1 cut(s) 348
HinP1I GCGC 1 cut(s) 348
HinfI GANTC 1 cut(s) 41
HpaII CCGG 3 cut(s) 242, 282, 303
HphI GGTGA 3 cut(s) 259, 268, 382
Hpy166II GTNNAC 2 cut(s) 364, 418
Hpy188I TCNGA 2 cut(s) 146, 297
Hpy188III TCNNGA 4 cut(s) 32, 38, 55, 328
Hpy8I GTNNAC 2 cut(s) 364, 418
Hpy99I CGWCG 2 cut(s) 19, 191
HpyAV CCTTC 2 cut(s) 146, 185
HpyCH4III ACNGT 1 cut(s) 29
HpyCH4V TGCA 2 cut(s) 84, 205
HpyF10VI GCNNNNNNNGC 1 cut(s) 227
HpyF3I CTNAG 1 cut(s) 143
Hsp92II CATG 3 cut(s) 71, 311, 337
HspAI GCGC 1 cut(s) 348
Kzo9I GATC 3 cut(s) 5, 34, 146
LweI GCATC 2 cut(s) 249, 421
MalI GATC 3 cut(s) 7, 36, 148
MboI GATC 3 cut(s) 5, 34, 146
MboII GAAGA 4 cut(s) 101, 141, 170, 388
MflI RGATCY 2 cut(s) 34, 146
MluCI AATT 1 cut(s) 104
MlyI GAGTC 1 cut(s) 35
MmeI TCCRAC 1 cut(s) 48
MnlI CCTC 6 cut(s) 110, 150, 194, 201, 306, 395
MseI TTAA 1 cut(s) 439
MspA1I CMGCKG 1 cut(s) 345
MspI CCGG 3 cut(s) 242, 282, 303
MspR9I CCNGG 3 cut(s) 282, 283, 303
MwoI GCNNNNNNNGC 1 cut(s) 227
NciI CCSGG 3 cut(s) 282, 283, 303
NdeII GATC 3 cut(s) 5, 34, 146
NlaIII CATG 3 cut(s) 71, 311, 337
NlaIV GGNNCC 2 cut(s) 73, 240
NspV TTCGAA 1 cut(s) 152
PkrI GCNGC 1 cut(s) 347
PleI GAGTC 1 cut(s) 35
PpsI GAGTC 1 cut(s) 35
PshAI GACNNNNGTC 1 cut(s) 237
PspN4I GGNNCC 2 cut(s) 73, 240
PspPI GGNCC 1 cut(s) 239
PsuI RGATCY 2 cut(s) 34, 146
RsaI GTAC 2 cut(s) 270, 363
RsaNI GTAC 2 cut(s) 269, 362
SaqAI TTAA 1 cut(s) 439
SatI GCNGC 1 cut(s) 346
Sau3AI GATC 3 cut(s) 5, 34, 146
Sau96I GGNCC 1 cut(s) 239
SchI GAGTC 1 cut(s) 35
ScrFI CCNGG 3 cut(s) 282, 283, 303
SetI ASST 2 cut(s) 138, 317
SfaNI GCATC 2 cut(s) 249, 421
SfuI TTCGAA 1 cut(s) 152
SinI GGWCC 1 cut(s) 239
SmaI CCCGGG 1 cut(s) 283
Sse9I AATT 1 cut(s) 104
SsiI CCGC 2 cut(s) 254, 345
StyD4I CCNGG 3 cut(s) 280, 281, 301
TaaI ACNGT 1 cut(s) 29
TaqI TCGA 2 cut(s) 17, 152
TasI AATT 1 cut(s) 104
TatI WGTACW 1 cut(s) 268
TauI GCSGC 1 cut(s) 348
Tru1I TTAA 1 cut(s) 439
Tru9I TTAA 1 cut(s) 439
TscAI CASTG 1 cut(s) 86
TspMI CCCGGG 1 cut(s) 281
TspRI CASTG 1 cut(s) 86
VpaK11BI GGWCC 1 cut(s) 239
XapI RAATTY 1 cut(s) 104
XmaI CCCGGG 1 cut(s) 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.