pycom14g18440

Partial alpha/beta-hydrolase lipase region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0011380
Physical Location & Seq
Reverse (-)
20095437 .. 20098875
3439 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1884 bp
ATGATTCAACGGTTCGTTGACAACATCATCGCGGTCACCAAAGAGTCAGTGAAGACGTTAACGTACGAATCGCTGAACAATATCGTGCGGCTGATCAATGGAATTTCGGCTCTGTTGCTGACGTTTCTGCCGGGGAAGGCGAACATTCTCGAAGGCATTCATGGATGGGAGCTCAGGCCCACCTTTCGCGGGCCCAGATTTCCTCGCTGGATGGAGAATGGGGTGTCCTCTTTCAATCGTTTTGTACACGAGCTTTCTGTGGATTCTGATTCTGATGGTTCAAACCTTGATTACGCATACGGAGATGAAGATAGTGATGATGGCATATCTCCTACATCACCATCCTCTCAAAGTTCAAGAGCCTCTCGGGCTTCTAGTTTCAGTAAGCGCGATAGGCGCTGGTCGTGGTGGATGTGGATCAGATACATATTTTTCTGGATTCTGTTTCCAGCAAGGTTTCTGCTTGGGATTCCAGTTCGCATTTTGCAATTGTCTTATACTAGTTCATCAAAAGCCTCTTCTGCCCCAGGAAGCAATGAGGCTTCGCAAACACACCCTATAAATAAAGTGCAGATTCTCAAGGATTACATTGTTCACCGATCCACTGATAGGAGACGTGGAGTCATTGAGGATCTTCATCTAGCAATCGAGATATTCATAGAAGCTGTTTTTGATGTTGTTCACAAGGCAGCACATTTTGTTCTTTCCCCTTCTGTGGCTTTTAAAACACTATCAGGATGGTGTTCACTGAGCAGCAGTGGTGTTCAAGATAGCCACAGTAGTGATGCAGAAGTTACTGTTCCGACAGCTACACTTGGAGAGAATGATCCAGCTCCAAAAGAAAGGACTACTTCATTTCAGCAGTCCCTAAATACCGATGCTCGGACTTGTCAGGATGTCATAACAGAGCTTGGGTACCCATATGAAGCTATTCATGTGATCACTAGTGATGGCTATGTTCTTCTCTTGGAAAGACTCCCGAGACGTGATTCCCGGAAAGCTGTTTATCTACAACATGGAATTTTGGATTCATCTATGGGTTGGGTGTCTAATGGGGTTGTTGGTTCACCGGCATTTGCGGCTTATGATCAAGGATATGATGTTTTCCTCGGTAACTTTCGTGGCTTGATTTCTAGGGAACATGTTAACAAAAATATCTCATCACGACAGTATTGGCAGTACTCCATCAATGAGCATGGGACTCAGGATATTCCTGCAATGATAGAGAAGATTCACCAAGTTAAAACTGCTGAATTGAAACTTAGTCAACCTGACGTTGACGAAGAAACAAATGATGGTCAACCGTACAAACTCTGTGCAATTTGCCATAGCTTGGGAGGAGCAGCAATACTTATGTACGTTATAACGCAGAGAATAGAAGAGAAACCCCACAGACTCTCTAGGCTGGTTTTATTGTCACCTGCTGGCTTCCATGGTGATTCCACCTTTATGTTTACAGTTGTGGAGAATGTCTTTCTTCTATTGGCTCCTCTTCTGGCTCCTCTTGTGCCTGCCTTCTACATACCAACCAGGTTCTTTCGCATGCTTCTGAACAAGTTGGCGCGAGACTTTCATAACTATCCTGCTGTCGGAGGACTTGTTCAGACCCTGATGAGTTATTTTGTTGGTGGGGATAGCTCAAATTGGGTTGGTGTGTTAGGGATACCACATTATAACATGAACGACATGCCGGGCGTATCATTCCGAGTGGCTCTCCATCTTGCACAAATCAAGCGTGCTGGAAGGTTTAGAATGTTCGATTACGGGAGTGGTTCTGCTAATATCGAAGTGTATGGATCGATCCAGAGCCCTTCGACTTGGGGGAGTACTATGGGCTTATTGATGTTCCAGTCGATCTGGTTGCTGGACGAAAGGACAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydro_lipase

Protein Analysis

628

Amino Acids

70.36

Weight (kDa)

6.69

Isoelectric Point (pI)

54.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydro_lipase PF04083 298 - 352 3.7e-14 Partial alpha/beta-hydrolase lipase region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011380)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18460 AT1G73920 AT1G73920
fragaria_vesca FvH4_5g12600 FvH4_5g12600
malus_domestica MD06G1212200.v1.1 MD14G1222700.v1.1
prunus_persica Prupe.5G218200_v2.0.a1
pyrus_communis pycom06g18940 pycom14g18440
rosa_chinensis RchiOBHm_Chr7g0184731
rosa_laevigata RLG00000004955
rosa_multiflora Rmu_ssc0000289.1_g000019
rosa_roxburghii Rroxscaffold_3G00269390
rosa_rugosa Rorug06G0464800
rosa_samantha Rh7AG078100 Rh7BG069200 Rh7CG070000 Rh7DG069400
rosa_wichuraiana Rw7G005730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1364, 1674
AarI CACCTGC 1 cut(s) 1429
Acc36I ACCTGC 1 cut(s) 1429
Acc65I GGTACC 1 cut(s) 915
AccB1I GGYRCC 1 cut(s) 915
AccB7I CCANNNNNTGG 1 cut(s) 1333
AccII CGCG 4 cut(s) 32, 189, 390, 1564
AciI CCGC 4 cut(s) 32, 88, 189, 1079
AclWI GGATC 6 cut(s) 425, 594, 639, 821, 1795, 1804
AcsI RAATTY 2 cut(s) 102, 1020
AfaI GTAC 7 cut(s) 65, 246, 917, 1181, 1307, 1358, 1828
AfiI CCNNNNNNNGG 6 cut(s) 189, 609, 715, 882, 1333, 1589
AflIII ACRYGT 1 cut(s) 1141
AgsI TTSAA 6 cut(s) 8, 235, 282, 357, 767, 1258
AhdI GACNNNNNGTC 1 cut(s) 620
AhlI ACTAGT 2 cut(s) 500, 944
AjiI CACGTC 2 cut(s) 617, 986
AjnI CCWGG 2 cut(s) 526, 1529
AleI CACNNNNGTG 1 cut(s) 780
AloI GAACNNNNNNTCC 2 cut(s) 1584, 1616
Alw21I GWGCWC 1 cut(s) 174
Alw26I GTCTC 3 cut(s) 607, 976, 1560
AlwI GGATC 6 cut(s) 425, 594, 639, 821, 1795, 1804
AlwNI CAGNNNCTG 1 cut(s) 1609
Ama87I CYCGRG 2 cut(s) 366, 979
AoxI GGCC 2 cut(s) 176, 191
ApaI GGGCCC 1 cut(s) 195
ApeKI GCWGC 3 cut(s) 689, 753, 1343
ApoI RAATTY 2 cut(s) 102, 1020
Asp700I GAANNNNTTC 2 cut(s) 156, 930
Asp718I GGTACC 1 cut(s) 915
AspLEI GCGC 3 cut(s) 390, 399, 1564
AspS9I GGNCC 3 cut(s) 177, 191, 192
AsuC2I CCSGG 3 cut(s) 132, 994, 1692
AsuHPI GGTGA 7 cut(s) 28, 330, 587, 1059, 1226, 1410, 1448
AvaI CYCGRG 2 cut(s) 366, 979
BaeGI GKGCMC 1 cut(s) 195
BanI GGYRCC 1 cut(s) 915
BanII GRGCYC 3 cut(s) 174, 195, 1811
BauI CACGAG 1 cut(s) 248
BbsI GAAGAC 1 cut(s) 59
Bbv12I GWGCWC 1 cut(s) 174
BbvI GCAGC 3 cut(s) 701, 765, 1355
BcgI CGANNNNNNTGC 2 cut(s) 1843, 1877
BciT130I CCWGG 2 cut(s) 528, 1531
BciVI GTATCC 1 cut(s) 1656
BclI TGATCA 3 cut(s) 93, 939, 1087
BcnI CCSGG 3 cut(s) 132, 994, 1692
BcoDI GTCTC 3 cut(s) 607, 976, 1560
BcuI ACTAGT 2 cut(s) 500, 944
BfaI CTAG 6 cut(s) 375, 501, 641, 945, 1134, 1401
BfoI RGCGCY 1 cut(s) 400
BfuAI ACCTGC 1 cut(s) 1429
BfuI GTATCC 1 cut(s) 1656
BglI GCCNNNNNGGC 1 cut(s) 368
BisI GCNGC 5 cut(s) 89, 690, 754, 1080, 1344
BlsI GCNGC 5 cut(s) 90, 691, 755, 1081, 1345
BmcAI AGTACT 2 cut(s) 1181, 1828
Bme1390I CCNGG 5 cut(s) 132, 528, 994, 1531, 1692
BmeRI GACNNNNNGTC 1 cut(s) 620
BmeT110I CYCGRG 2 cut(s) 366, 979
BmgBI CACGTC 2 cut(s) 617, 986
BmgT120I GGNCC 3 cut(s) 177, 191, 192
BmiI GGNNCC 4 cut(s) 193, 917, 1488, 1500
BmrFI CCNGG 5 cut(s) 132, 528, 994, 1531, 1692
BmsI GCATC 2 cut(s) 775, 868
BpiI GAAGAC 1 cut(s) 59
BplI GAGNNNNNCTC 2 cut(s) 1698, 1730
Bpu10I CCTNAGC 1 cut(s) 173
BpuEI CTTGAG 1 cut(s) 563
BpuMI CCSGG 3 cut(s) 132, 994, 1692
Bsa29I ATCGAT 1 cut(s) 1799
BsaBI GATNNNNATC 2 cut(s) 416, 636
BsaJI CCNNGG 4 cut(s) 131, 526, 1108, 1432
BsaXI ACNNNNNCTCC 2 cut(s) 1584, 1614
Bsc4I CCNNNNNNNGG 6 cut(s) 189, 609, 715, 882, 1333, 1589
Bse118I RCCGGY 1 cut(s) 1069
Bse1I ACTGG 2 cut(s) 473, 1849
Bse3DI GCAATG 2 cut(s) 541, 1224
Bse8I GATNNNNATC 2 cut(s) 416, 636
BseBI CCWGG 2 cut(s) 528, 1531
BseCI ATCGAT 1 cut(s) 1799
BseDI CCNNGG 4 cut(s) 131, 526, 1108, 1432
BseGI GGATG 6 cut(s) 170, 216, 341, 417, 743, 901
BseJI GATNNNNATC 2 cut(s) 416, 636
BseLI CCNNNNNNNGG 6 cut(s) 189, 609, 715, 882, 1333, 1589
BseMI GCAATG 2 cut(s) 541, 1224
BseMII CTCAG 3 cut(s) 187, 740, 1217
BseNI ACTGG 2 cut(s) 473, 1849
BseRI GAGGAG 3 cut(s) 1353, 1479, 1491
BseSI GKGCMC 1 cut(s) 195
BseXI GCAGC 3 cut(s) 701, 765, 1355
BsgI GTGCAG 1 cut(s) 590
Bsh1236I CGCG 4 cut(s) 32, 189, 390, 1564
BshFI GGCC 2 cut(s) 178, 193
BshNI GGYRCC 1 cut(s) 915
BshVI ATCGAT 1 cut(s) 1799
BsiHKAI GWGCWC 1 cut(s) 174
BsiHKCI CYCGRG 2 cut(s) 366, 979
BsiSI CCGG 4 cut(s) 131, 994, 1070, 1691
BsiWI CGTACG 1 cut(s) 63
BslFI GGGAC 2 cut(s) 850, 1213
BslI CCNNNNNNNGG 6 cut(s) 189, 609, 715, 882, 1333, 1589
BsmAI GTCTC 3 cut(s) 607, 976, 1560
BsmBI CGTCTC 2 cut(s) 607, 976
BsmFI GGGAC 2 cut(s) 850, 1213
BsmI GAATGC 1 cut(s) 156
BsnI GGCC 2 cut(s) 178, 193
BsoBI CYCGRG 2 cut(s) 366, 979
Bsp120I GGGCCC 1 cut(s) 191
Bsp1286I GDGCHC 3 cut(s) 174, 195, 1811
Bsp1407I TGTACA 1 cut(s) 244
Bsp19I CCATGG 1 cut(s) 1432
BspACI CCGC 4 cut(s) 32, 88, 189, 1079
BspANI GGCC 2 cut(s) 178, 193
BspCNI CTCAG 3 cut(s) 186, 741, 1216
BspDI ATCGAT 1 cut(s) 1799
BspFNI CGCG 4 cut(s) 32, 189, 390, 1564
BspLI GGNNCC 4 cut(s) 193, 917, 1488, 1500
BspMI ACCTGC 1 cut(s) 1429
BspPI GGATC 6 cut(s) 425, 594, 639, 821, 1795, 1804
BspT107I GGYRCC 1 cut(s) 915
BsrDI GCAATG 2 cut(s) 541, 1224
BsrFI RCCGGY 1 cut(s) 1069
BsrGI TGTACA 1 cut(s) 244
BsrI ACTGG 2 cut(s) 473, 1849
BssAI RCCGGY 1 cut(s) 1069
BssECI CCNNGG 4 cut(s) 131, 526, 1108, 1432
BssSI CACGAG 1 cut(s) 248
BssT1I CCWWGG 1 cut(s) 1432
Bst2BI CACGAG 1 cut(s) 248
Bst2UI CCWGG 2 cut(s) 528, 1531
Bst4CI ACNGT 6 cut(s) 12, 779, 799, 1170, 1305, 1459
Bst6I CTCTTC 3 cut(s) 523, 1374, 1497
BstAUI TGTACA 1 cut(s) 244
BstC8I GCNNGC 5 cut(s) 191, 1426, 1512, 1544, 1737
BstDEI CTNAG 4 cut(s) 173, 749, 1203, 1262
BstDSI CCRYGG 1 cut(s) 1432
BstEII GGTNACC 1 cut(s) 34
BstF5I GGATG 6 cut(s) 170, 216, 341, 417, 743, 901
BstFNI CGCG 4 cut(s) 32, 189, 390, 1564
BstH2I RGCGCY 1 cut(s) 400
BstHHI GCGC 3 cut(s) 390, 399, 1564
BstMAI GTCTC 3 cut(s) 607, 976, 1560
BstMWI GCNNNNNNNGC 5 cut(s) 368, 394, 396, 521, 1079
BstNI CCWGG 2 cut(s) 528, 1531
BstNSI RCATGY 3 cut(s) 1145, 1546, 1690
BstPI GGTNACC 1 cut(s) 34
BstSCI CCNGG 5 cut(s) 130, 526, 992, 1529, 1690
BstSLI GKGCMC 1 cut(s) 195
BstUI CGCG 4 cut(s) 32, 189, 390, 1564
BstV1I GCAGC 3 cut(s) 701, 765, 1355
BstV2I GAAGAC 1 cut(s) 59
BstX2I RGATCY 1 cut(s) 631
BstYI RGATCY 1 cut(s) 631
Bsu15I ATCGAT 1 cut(s) 1799
BsuI GTATCC 1 cut(s) 1656
BsuRI GGCC 2 cut(s) 178, 193
BsuTUI ATCGAT 1 cut(s) 1799
BtgI CCRYGG 1 cut(s) 1432
BtgZI GCGATG 1 cut(s) 13
BtrI CACGTC 2 cut(s) 617, 986
BtsCI GGATG 6 cut(s) 170, 216, 341, 417, 743, 901
BtsI GCAGTG 1 cut(s) 763
BtsIMutI CAGTG 4 cut(s) 54, 603, 746, 763
BveI ACCTGC 1 cut(s) 1429
Cac8I GCNNGC 5 cut(s) 191, 1426, 1512, 1544, 1737
CaiI CAGNNNCTG 1 cut(s) 1609
CfoI GCGC 3 cut(s) 390, 399, 1564
Cfr10I RCCGGY 1 cut(s) 1069
Cfr13I GGNCC 3 cut(s) 177, 191, 192
ClaI ATCGAT 1 cut(s) 1799
CsiI ACCWGGT 1 cut(s) 1529
Csp6I GTAC 7 cut(s) 64, 245, 916, 1180, 1306, 1357, 1827
CviAII CATG 9 cut(s) 161, 935, 1016, 1142, 1196, 1433, 1543, 1678, 1687
CviQI GTAC 7 cut(s) 64, 245, 916, 1180, 1306, 1357, 1827
DdeI CTNAG 4 cut(s) 173, 749, 1203, 1262
DraI TTTAAA 1 cut(s) 724
DriI GACNNNNNGTC 1 cut(s) 620
Eam1104I CTCTTC 3 cut(s) 523, 1374, 1497
Eam1105I GACNNNNNGTC 1 cut(s) 620
EarI CTCTTC 3 cut(s) 523, 1374, 1497
Ecl136II GAGCTC 1 cut(s) 172
Eco130I CCWWGG 1 cut(s) 1432
Eco24I GRGCYC 3 cut(s) 174, 195, 1811
Eco53kI GAGCTC 1 cut(s) 172
Eco88I CYCGRG 2 cut(s) 366, 979
Eco91I GGTNACC 1 cut(s) 34
EcoICRI GAGCTC 1 cut(s) 172
EcoO65I GGTNACC 1 cut(s) 34
EcoRII CCWGG 2 cut(s) 526, 1529
EcoT14I CCWWGG 1 cut(s) 1432
EcoT38I GRGCYC 3 cut(s) 174, 195, 1811
ErhI CCWWGG 1 cut(s) 1432
Esp3I CGTCTC 2 cut(s) 607, 976
FaeI CATG 9 cut(s) 164, 938, 1019, 1145, 1199, 1436, 1546, 1681, 1690
FalI AAGNNNNNCTT 2 cut(s) 835, 867
FaqI GGGAC 2 cut(s) 850, 1213
FatI CATG 9 cut(s) 160, 934, 1015, 1141, 1195, 1432, 1542, 1677, 1686
FauI CCCGC 1 cut(s) 182
FauNDI CATATG 1 cut(s) 922
FbaI TGATCA 3 cut(s) 93, 939, 1087
Fnu4HI GCNGC 5 cut(s) 89, 690, 754, 1080, 1344
FokI GGATG 6 cut(s) 177, 223, 328, 424, 750, 908
FriOI GRGCYC 3 cut(s) 174, 195, 1811
Fsp4HI GCNGC 5 cut(s) 89, 690, 754, 1080, 1344
FspBI CTAG 6 cut(s) 375, 501, 641, 945, 1134, 1401
GlaI GCGC 3 cut(s) 389, 398, 1563
GluI GCNGC 5 cut(s) 89, 690, 754, 1080, 1344
HaeII RGCGCY 1 cut(s) 400
HaeIII GGCC 2 cut(s) 178, 193
HapII CCGG 4 cut(s) 131, 994, 1070, 1691
HhaI GCGC 3 cut(s) 390, 399, 1564
Hin1II CATG 9 cut(s) 164, 938, 1019, 1145, 1199, 1436, 1546, 1681, 1690
Hin6I GCGC 3 cut(s) 388, 397, 1562
HinP1I GCGC 3 cut(s) 388, 397, 1562
HincII GTYRAC 6 cut(s) 19, 60, 1147, 1268, 1279, 1301
HindII GTYRAC 6 cut(s) 19, 60, 1147, 1268, 1279, 1301
HpaI GTTAAC 2 cut(s) 60, 1147
HpaII CCGG 4 cut(s) 131, 994, 1070, 1691
HphI GGTGA 7 cut(s) 28, 330, 587, 1059, 1226, 1410, 1448
Hpy188I TCNGA 9 cut(s) 268, 274, 422, 804, 885, 1551, 1592, 1605, 1706
HpyAV CCTTC 6 cut(s) 130, 146, 720, 1525, 1737, 1821
HpyCH4III ACNGT 6 cut(s) 12, 779, 799, 1170, 1305, 1459
HpyCH4IV ACGT 7 cut(s) 56, 62, 122, 616, 985, 1275, 1359
HpyCH4V TGCA 6 cut(s) 487, 571, 788, 1217, 1319, 1724
HpyF10VI GCNNNNNNNGC 5 cut(s) 368, 394, 396, 521, 1079
HpyF3I CTNAG 4 cut(s) 173, 749, 1203, 1262
HpySE526I ACGT 7 cut(s) 56, 62, 122, 616, 985, 1275, 1359
Hsp92II CATG 9 cut(s) 164, 938, 1019, 1145, 1199, 1436, 1546, 1681, 1690
HspAI GCGC 3 cut(s) 388, 397, 1562
KpnI GGTACC 1 cut(s) 919
Ksp22I TGATCA 3 cut(s) 93, 939, 1087
KspAI GTTAAC 2 cut(s) 60, 1147
LmnI GCTCC 5 cut(s) 169, 838, 1340, 1492, 1504
Lsp1109I GCAGC 3 cut(s) 701, 765, 1355
LweI GCATC 2 cut(s) 775, 868
MabI ACCWGGT 1 cut(s) 1529
MaeI CTAG 6 cut(s) 375, 501, 641, 945, 1134, 1401
MaeII ACGT 7 cut(s) 56, 62, 122, 616, 985, 1275, 1359
MaeIII GTNAC 4 cut(s) 34, 793, 1112, 1416
MfeI CAATTG 1 cut(s) 488
MflI RGATCY 1 cut(s) 631
MhlI GDGCHC 3 cut(s) 174, 195, 1811
MluCI AATT 6 cut(s) 102, 488, 1020, 1253, 1320, 1642
MlyI GAGTC 5 cut(s) 53, 630, 969, 1195, 1389
MmeI TCCRAC 2 cut(s) 827, 1570
MroXI GAANNNNTTC 2 cut(s) 156, 930
MseI TTAA 4 cut(s) 59, 723, 1146, 1242
MslI CAYNNNNRTG 2 cut(s) 780, 1448
MspI CCGG 4 cut(s) 131, 994, 1070, 1691
MspR9I CCNGG 5 cut(s) 132, 528, 994, 1531, 1692
MunI CAATTG 1 cut(s) 488
Mva1269I GAATGC 1 cut(s) 156
MvaI CCWGG 2 cut(s) 528, 1531
MvnI CGCG 4 cut(s) 32, 189, 390, 1564
MwoI GCNNNNNNNGC 5 cut(s) 368, 394, 396, 521, 1079
NciI CCSGG 3 cut(s) 132, 994, 1692
NcoI CCATGG 1 cut(s) 1432
NdeI CATATG 1 cut(s) 922
NlaIII CATG 9 cut(s) 164, 938, 1019, 1145, 1199, 1436, 1546, 1681, 1690
NlaIV GGNNCC 4 cut(s) 193, 917, 1488, 1500
NmuCI GTSAC 2 cut(s) 34, 1416
NspI RCATGY 3 cut(s) 1145, 1546, 1690
OliI CACNNNNGTG 1 cut(s) 780
PaeI GCATGC 1 cut(s) 1546
PaqCI CACCTGC 1 cut(s) 1429
PciI ACATGT 1 cut(s) 1141
PctI GAATGC 1 cut(s) 156
PdmI GAANNNNTTC 2 cut(s) 156, 930
Pfl23II CGTACG 1 cut(s) 63
PflMI CCANNNNNTGG 1 cut(s) 1333
PfoI TCCNGGA 1 cut(s) 992
PkrI GCNGC 5 cut(s) 90, 691, 755, 1081, 1345
PleI GAGTC 5 cut(s) 52, 629, 969, 1195, 1389
PpsI GAGTC 5 cut(s) 52, 629, 969, 1195, 1389
PscI ACATGT 1 cut(s) 1141
PsiI TTATAA 2 cut(s) 1364, 1674
Psp124BI GAGCTC 1 cut(s) 174
Psp6I CCWGG 2 cut(s) 526, 1529
PspEI GGTNACC 1 cut(s) 34
PspGI CCWGG 2 cut(s) 526, 1529
PspLI CGTACG 1 cut(s) 63
PspN4I GGNNCC 4 cut(s) 193, 917, 1488, 1500
PspOMI GGGCCC 1 cut(s) 191
PspPI GGNCC 3 cut(s) 177, 191, 192
PstNI CAGNNNCTG 1 cut(s) 1609
PsuI RGATCY 1 cut(s) 631
RsaI GTAC 7 cut(s) 65, 246, 917, 1181, 1307, 1358, 1828
RsaNI GTAC 7 cut(s) 64, 245, 916, 1180, 1306, 1357, 1827
RseI CAYNNNNRTG 2 cut(s) 780, 1448
SacI GAGCTC 1 cut(s) 174
SaqAI TTAA 4 cut(s) 59, 723, 1146, 1242
SatI GCNGC 5 cut(s) 89, 690, 754, 1080, 1344
Sau96I GGNCC 3 cut(s) 177, 191, 192
ScaI AGTACT 2 cut(s) 1181, 1828
SchI GAGTC 5 cut(s) 53, 630, 969, 1195, 1389
ScrFI CCNGG 5 cut(s) 132, 528, 994, 1531, 1692
SduI GDGCHC 3 cut(s) 174, 195, 1811
SexAI ACCWGGT 1 cut(s) 1529
SfaNI GCATC 2 cut(s) 775, 868
SmiMI CAYNNNNRTG 2 cut(s) 780, 1448
SmlI CTYRAG 1 cut(s) 578
SmoI CTYRAG 1 cut(s) 578
SpeI ACTAGT 2 cut(s) 500, 944
SphI GCATGC 1 cut(s) 1546
Sse9I AATT 6 cut(s) 102, 488, 1020, 1253, 1320, 1642
SsiI CCGC 4 cut(s) 32, 88, 189, 1079
SspMI CTAG 6 cut(s) 375, 501, 641, 945, 1134, 1401
SstI GAGCTC 1 cut(s) 174
StyD4I CCNGG 5 cut(s) 130, 526, 992, 1529, 1690
StyI CCWWGG 1 cut(s) 1432
TaaI ACNGT 6 cut(s) 12, 779, 799, 1170, 1305, 1459
TaiI ACGT 7 cut(s) 59, 65, 125, 619, 988, 1278, 1362
TaqI TCGA 7 cut(s) 150, 648, 1758, 1785, 1799, 1814, 1853
TasI AATT 6 cut(s) 102, 488, 1020, 1253, 1320, 1642
TatI WGTACW 3 cut(s) 244, 1179, 1826
TauI GCSGC 2 cut(s) 91, 1082
Tru1I TTAA 4 cut(s) 59, 723, 1146, 1242
Tru9I TTAA 4 cut(s) 59, 723, 1146, 1242
TscAI CASTG 4 cut(s) 54, 610, 753, 763
TseFI GTSAC 2 cut(s) 34, 1416
TseI GCWGC 3 cut(s) 689, 753, 1343
Tsp45I GTSAC 2 cut(s) 34, 1416
TspGWI ACGGA 1 cut(s) 315
TspRI CASTG 4 cut(s) 54, 610, 753, 763
Van91I CCANNNNNTGG 1 cut(s) 1333
XapI RAATTY 2 cut(s) 102, 1020
XceI RCATGY 3 cut(s) 1145, 1546, 1690
XmnI GAANNNNTTC 2 cut(s) 156, 930
XspI CTAG 6 cut(s) 375, 501, 641, 945, 1134, 1401
ZrmI AGTACT 2 cut(s) 1181, 1828
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.