pycom15g00910

Synaptotagmin-like mitochondrial-lipid-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008282
Physical Location & Seq
Forward (+)
482416 .. 483734
1319 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 636 bp
ATGGTTTATGATTGGGAAAAGATTGGCAAACATGACAAGATGGGTATAAATGTCATTCCACTGAAAGAACTTACGCCTGAAGAGCCTAAAGTTATGACTCTTGACGTGCTGAAGAACATGGACCTGAATGATACTCAAAATGAGAAATCACGTGGGCAGCTTGTTGTGGAATTGATCTATAAACCCTTTACACAGGATGAGATGCCTAAAGATGATGAAGATCCAAATGCTATAGAAAAGGCTCCTGAAGGAACACCTGCAACTGGTGGTGTGCTTGTAATTACCGTCCACGAAGCTGAAGATGTGGAAGGAAAGCACCACACTAACCCACAAGTGCGCATACTTTTCAGAGGGGAAGAGAAAAGAACCAAGCTTGTCAAGAAAAATCGGGATCCAAGATGGGATGAGGAGTTTCAGTTTATTTTAGAAGAGCCACCGAAAAATGATAGGCTTCATGTTGAAGTGGTCAGCCACTCCACAAGGATGGGGCTGTTGCATCCCAAGGAAACTCTTGGATACGTGGATATAAACTTGGCGGATGTTGTTAGCAACAGAAGAATCAATGAAAAATACCATTTGATAGACTCGAAGAATGGACGCCTTCAACTCGAGCTGCAATGGAGAACATCTTCTTGA

Protein Analysis

212

Amino Acids

24.45

Weight (kDa)

5.55

Isoelectric Point (pI)

36.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 90 - 193 2.1e-15 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 265
Acc16I TGCGCA 1 cut(s) 338
Acc36I ACCTGC 1 cut(s) 265
AciI CCGC 1 cut(s) 536
AclWI GGATC 3 cut(s) 215, 386, 399
AcuI CTGAAG 4 cut(s) 99, 131, 267, 318
AcvI CACGTG 1 cut(s) 152
AcyI GRCGYC 1 cut(s) 598
AfiI CCNNNNNNNGG 1 cut(s) 263
AgsI TTSAA 2 cut(s) 461, 605
AjiI CACGTC 1 cut(s) 106
AluBI AGCT 4 cut(s) 160, 296, 373, 613
AluI AGCT 4 cut(s) 160, 296, 373, 613
AlwI GGATC 3 cut(s) 215, 386, 399
Ama87I CYCGRG 1 cut(s) 608
ApeKI GCWGC 2 cut(s) 157, 613
Asp700I GAANNNNTTC 1 cut(s) 628
AspLEI GCGC 1 cut(s) 339
AspS9I GGNCC 1 cut(s) 121
AvaI CYCGRG 1 cut(s) 608
AvaII GGWCC 1 cut(s) 121
BamHI GGATCC 1 cut(s) 391
BbrPI CACGTG 1 cut(s) 152
BbvI GCAGC 2 cut(s) 169, 600
BccI CCATC 3 cut(s) 34, 393, 478
BciVI GTATCC 1 cut(s) 509
BfmI CTRYAG 1 cut(s) 231
BfuAI ACCTGC 1 cut(s) 265
BfuI GTATCC 1 cut(s) 509
BisI GCNGC 2 cut(s) 158, 614
BlsI GCNGC 2 cut(s) 159, 615
Bme18I GGWCC 1 cut(s) 121
BmeT110I CYCGRG 1 cut(s) 608
BmgBI CACGTC 1 cut(s) 106
BmgT120I GGNCC 1 cut(s) 121
BmiI GGNNCC 2 cut(s) 243, 393
BmsI GCATC 2 cut(s) 192, 505
BsaAI YACGTR 2 cut(s) 152, 520
BsaBI GATNNNNATC 1 cut(s) 219
BsaHI GRCGYC 1 cut(s) 598
BsaJI CCNNGG 1 cut(s) 501
BsaXI ACNNNNNCTCC 2 cut(s) 401, 431
Bsc4I CCNNNNNNNGG 1 cut(s) 263
Bse1I ACTGG 1 cut(s) 268
Bse3DI GCAATG 1 cut(s) 623
Bse8I GATNNNNATC 1 cut(s) 219
BseDI CCNNGG 1 cut(s) 501
BseGI GGATG 5 cut(s) 202, 409, 489, 496, 544
BseJI GATNNNNATC 1 cut(s) 219
BseLI CCNNNNNNNGG 1 cut(s) 263
BseMI GCAATG 1 cut(s) 623
BseNI ACTGG 1 cut(s) 268
BseRI GAGGAG 1 cut(s) 422
BseXI GCAGC 2 cut(s) 169, 600
BsiHKCI CYCGRG 1 cut(s) 608
BslI CCNNNNNNNGG 1 cut(s) 263
BsoBI CYCGRG 1 cut(s) 608
Bsp143I GATC 3 cut(s) 174, 220, 391
BspACI CCGC 1 cut(s) 536
BspLI GGNNCC 2 cut(s) 243, 393
BspMI ACCTGC 1 cut(s) 265
BspPI GGATC 3 cut(s) 215, 386, 399
BspQI GCTCTTC 2 cut(s) 75, 423
BsrDI GCAATG 1 cut(s) 623
BsrI ACTGG 1 cut(s) 268
BssECI CCNNGG 1 cut(s) 501
BssMI GATC 3 cut(s) 174, 220, 391
BssNI GRCGYC 1 cut(s) 598
BssT1I CCWWGG 1 cut(s) 501
Bst4CI ACNGT 1 cut(s) 286
Bst6I CTCTTC 3 cut(s) 75, 351, 423
BstACI GRCGYC 1 cut(s) 598
BstBAI YACGTR 2 cut(s) 152, 520
BstF5I GGATG 5 cut(s) 202, 409, 489, 496, 544
BstHHI GCGC 1 cut(s) 339
BstKTI GATC 3 cut(s) 177, 223, 394
BstMBI GATC 3 cut(s) 174, 220, 391
BstMWI GCNNNNNNNGC 1 cut(s) 82
BstSFI CTRYAG 1 cut(s) 231
BstV1I GCAGC 2 cut(s) 169, 600
BstX2I RGATCY 2 cut(s) 220, 391
BstXI CCANNNNNNTGG 1 cut(s) 484
BstYI RGATCY 2 cut(s) 220, 391
BsuI GTATCC 1 cut(s) 509
BtrI CACGTC 1 cut(s) 106
BtsCI GGATG 5 cut(s) 202, 409, 489, 496, 544
BtsIMutI CAGTG 1 cut(s) 59
BveI ACCTGC 1 cut(s) 265
CfoI GCGC 1 cut(s) 339
Cfr13I GGNCC 1 cut(s) 121
CseI GACGC 1 cut(s) 606
CviAII CATG 3 cut(s) 32, 118, 455
DpnI GATC 3 cut(s) 176, 222, 393
DpnII GATC 3 cut(s) 174, 220, 391
Eam1104I CTCTTC 3 cut(s) 75, 351, 423
EarI CTCTTC 3 cut(s) 75, 351, 423
EciI GGCGGA 1 cut(s) 551
Eco130I CCWWGG 1 cut(s) 501
Eco47I GGWCC 1 cut(s) 121
Eco57I CTGAAG 4 cut(s) 99, 131, 267, 318
Eco72I CACGTG 1 cut(s) 152
Eco88I CYCGRG 1 cut(s) 608
EcoT14I CCWWGG 1 cut(s) 501
ErhI CCWWGG 1 cut(s) 501
FaeI CATG 3 cut(s) 35, 121, 458
FatI CATG 3 cut(s) 31, 117, 454
Fnu4HI GCNGC 2 cut(s) 158, 614
FokI GGATG 5 cut(s) 209, 416, 483, 496, 551
Fsp4HI GCNGC 2 cut(s) 158, 614
FspAI RTGCGCAY 1 cut(s) 338
FspI TGCGCA 1 cut(s) 338
GlaI GCGC 1 cut(s) 338
GluI GCNGC 2 cut(s) 158, 614
HgaI GACGC 1 cut(s) 606
HhaI GCGC 1 cut(s) 339
Hin1I GRCGYC 1 cut(s) 598
Hin1II CATG 3 cut(s) 35, 121, 458
Hin6I GCGC 1 cut(s) 337
HinP1I GCGC 1 cut(s) 337
HindIII AAGCTT 1 cut(s) 371
HinfI GANTC 3 cut(s) 97, 558, 584
Hpy166II GTNNAC 1 cut(s) 289
Hpy188I TCNGA 1 cut(s) 350
Hpy188III TCNNGA 5 cut(s) 101, 245, 379, 389, 633
Hpy8I GTNNAC 1 cut(s) 289
HpyAV CCTTC 3 cut(s) 242, 302, 611
HpyCH4III ACNGT 1 cut(s) 286
HpyCH4IV ACGT 3 cut(s) 105, 151, 519
HpyCH4V TGCA 3 cut(s) 260, 496, 616
HpyF10VI GCNNNNNNNGC 1 cut(s) 82
HpySE526I ACGT 3 cut(s) 105, 151, 519
Hsp92I GRCGYC 1 cut(s) 598
Hsp92II CATG 3 cut(s) 35, 121, 458
HspAI GCGC 1 cut(s) 337
Kzo9I GATC 3 cut(s) 174, 220, 391
LguI GCTCTTC 2 cut(s) 75, 423
LmnI GCTCC 1 cut(s) 247
LpnPI CCDG 6 cut(s) 90, 137, 179, 249, 258, 270
Lsp1109I GCAGC 2 cut(s) 169, 600
LweI GCATC 2 cut(s) 192, 505
MaeII ACGT 3 cut(s) 105, 151, 519
MalI GATC 3 cut(s) 176, 222, 393
MboI GATC 3 cut(s) 174, 220, 391
MboII GAAGA 9 cut(s) 92, 124, 230, 311, 368, 440, 567, 601, 621
MflI RGATCY 2 cut(s) 220, 391
MluCI AATT 2 cut(s) 170, 279
MlyI GAGTC 2 cut(s) 91, 578
MnlI CCTC 2 cut(s) 344, 400
MroXI GAANNNNTTC 1 cut(s) 628
MslI CAYNNNNRTG 1 cut(s) 482
MwoI GCNNNNNNNGC 1 cut(s) 82
NdeII GATC 3 cut(s) 174, 220, 391
NlaIII CATG 3 cut(s) 35, 121, 458
NlaIV GGNNCC 2 cut(s) 243, 393
NsbI TGCGCA 1 cut(s) 338
PaeR7I CTCGAG 1 cut(s) 608
PaqCI CACCTGC 1 cut(s) 265
PciSI GCTCTTC 2 cut(s) 75, 423
PdmI GAANNNNTTC 1 cut(s) 628
PfeI GAWTC 1 cut(s) 558
PkrI GCNGC 2 cut(s) 159, 615
PleI GAGTC 2 cut(s) 91, 578
PmaCI CACGTG 1 cut(s) 152
PmlI CACGTG 1 cut(s) 152
PpsI GAGTC 2 cut(s) 91, 578
Ppu21I YACGTR 2 cut(s) 152, 520
PspCI CACGTG 1 cut(s) 152
PspN4I GGNNCC 2 cut(s) 243, 393
PspPI GGNCC 1 cut(s) 121
PspXI VCTCGAGB 1 cut(s) 608
PsuI RGATCY 2 cut(s) 220, 391
RseI CAYNNNNRTG 1 cut(s) 482
SapI GCTCTTC 2 cut(s) 75, 423
SatI GCNGC 2 cut(s) 158, 614
Sau3AI GATC 3 cut(s) 174, 220, 391
Sau96I GGNCC 1 cut(s) 121
SchI GAGTC 2 cut(s) 91, 578
SetI ASST 9 cut(s) 108, 126, 154, 162, 259, 298, 375, 522, 615
SfaNI GCATC 2 cut(s) 192, 505
SfcI CTRYAG 1 cut(s) 231
Sfr274I CTCGAG 1 cut(s) 608
SinI GGWCC 1 cut(s) 121
SlaI CTCGAG 1 cut(s) 608
SmiMI CAYNNNNRTG 1 cut(s) 482
SmlI CTYRAG 1 cut(s) 608
SmoI CTYRAG 1 cut(s) 608
Sse9I AATT 2 cut(s) 170, 279
SsiI CCGC 1 cut(s) 536
StyI CCWWGG 1 cut(s) 501
TaaI ACNGT 1 cut(s) 286
TaiI ACGT 3 cut(s) 108, 154, 522
TaqI TCGA 2 cut(s) 587, 609
TasI AATT 2 cut(s) 170, 279
TfiI GAWTC 1 cut(s) 558
TscAI CASTG 1 cut(s) 66
TseI GCWGC 2 cut(s) 157, 613
TspDTI ATGAA 3 cut(s) 231, 443, 579
TspRI CASTG 1 cut(s) 66
VpaK11BI GGWCC 1 cut(s) 121
XhoI CTCGAG 1 cut(s) 608
XmnI GAANNNNTTC 1 cut(s) 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.