Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0008282

Physical Location & Seq

Genomic Coordinates Reverse (-)

47136707 .. 47138021

Gene Structure 1315 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0319600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 987 bp

ATGTGGTCCATCGGATATCCTCGATATGCAAGGAAAGATTTATTCGATAAAGTGGGATTGGTGAAAGGCAATTATCGACCCACGTACATAGTGGAGAAATATGTGTATGGAGCACCAAAACTGCGATGTCGCCCATCCTACCTGTTGGCTTACGTTGCGGTGTCAGACAATGAAGGAACTAAAGCCTTGGGGAGGAGGGACATTCTGATTGCCTGGAGAGGAACCAAGTTGGAGGCTGAAGTTGCTGTCGACAAAAACACCGAACTACGCCCGGCTCCTAAAATTTTTGGAAACCAGTATGCCGGCGCTATGGTGCACGGTGGTTGGTATGATTATTACATTAATACTGACGTTAGGACCCCCAAGAACCAACGAACTAGTTGCCAAGATCAGGTTGGAGAGGCCGTTAGAAAACTGATAGATCAATACGGTAACGAGGGTCAACAACTCAGCATTACTATAACTGGCCGCGGCACTGGTGCTGCATTTGCAATTTTAAATGGGATGGATATTGTTTGCAACAAACTCAATTATCCTACAAAACAACCCAAGAGCCCATGTCTGGTCACGGCGATTGTCTTTGGCTGTCCCTTTGTTGGAAACGAAGCCTTTAAGAATGCGGAGAACGATGAGACCATGTTTCAGATCTTGCGGGTCACAAATGAACCAGATCCAGTGACTAATTCGGAGCCTTCTCCGGAATATGAACATATTGGAGTAGAGGTGAAAATTAACACAGTCAATTCGCCCTACTTGAAGAATCCAATAGATACCGTACATGAGTTGGAGGTTTACTTGCATGGGGTTGCAGCGCACCCAGGGACAACTGTGGCATTGGAAGATATCCGCCGTACAGTGATGGTGAACAAAACATTGGACGGTCTTAAGGATGAAACTATAGTCCCCCCCATCTGGATTTTAAATAAGTTCATGGTTCAAAATGAGAGAGGCGCCTGGGAGCTGAAGGATGTTCCCGTTGATGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0012505 GO:0016020 GO:0031224 GO:0031227 GO:0031984 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044232 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0098827

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

C2 SMP_LBD

Protein Analysis

328

Amino Acids

36.79

Weight (kDa)

6.26

Isoelectric Point (pI)

25.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lipase_3	PF01764	70 - 229	1.4e-21	Lipase (class 3)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0008282)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20080 AT1G20080 AT1G20080 AT1G20080 AT1G20080
fragaria_vesca	FvH4_2g38220
malus_domestica	MD08G1010600.v1.1 MD15G1010700.v1.1
prunus_persica	Prupe.1G363200_v2.0.a1 Prupe.1G363200_v2.0.a1
pyrus_communis	pycom08g00920 pycom15g00900 pycom15g00910
rosa_chinensis	RchiOBHm_Chr6g0303161
rosa_laevigata	RLG00000011101
rosa_multiflora	Rmu_sc0007936.1_g000002
rosa_roxburghii	Rroxscaffold_7G00164960
rosa_rugosa	Rorug06G0319500 Rorug06G0319600
rosa_samantha	Rh6AG433500 Rh6BG469200 Rh6DG432600
rosa_wichuraiana	Rw6G037590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	950
AccI	GTMKAC	1 cut(s)	249
AccII	CGCG	1 cut(s)	471
AccIII	TCCGGA	1 cut(s)	697
AciI	CCGC	6 cut(s)	158, 469, 471, 620, 652, 847
AclWI	GGATC	1 cut(s)	665
AcoI	YGGCCR	1 cut(s)	466
AcsI	RAATTY	1 cut(s)	282
AcuI	CTGAAG	2 cut(s)	258, 983
AcyI	GRCGYC	1 cut(s)	951
AfaI	GTAC	3 cut(s)	86, 777, 853
AfiI	CCNNNNNNNGG	5 cut(s)	192, 391, 562, 596, 912
AflII	CTTAAG	1 cut(s)	884
AgsI	TTSAA	2 cut(s)	757, 938
AhlI	ACTAGT	1 cut(s)	377
AjnI	CCWGG	3 cut(s)	212, 817, 953
AjuI	GAANNNNNNNTTGG	2 cut(s)	857, 889
AluBI	AGCT	1 cut(s)	961
AluI	AGCT	1 cut(s)	961
Alw21I	GWGCWC	2 cut(s)	115, 318
Alw26I	GTCTC	1 cut(s)	626
Alw44I	GTGCAC	1 cut(s)	314
AlwI	GGATC	1 cut(s)	665
Aor13HI	TCCGGA	1 cut(s)	697
AoxI	GGCC	2 cut(s)	402, 466
ApaLI	GTGCAC	1 cut(s)	314
ApeKI	GCWGC	2 cut(s)	482, 809
ApoI	RAATTY	1 cut(s)	282
AseI	ATTAAT	1 cut(s)	342
AspLEI	GCGC	3 cut(s)	308, 814, 953
AspS9I	GGNCC	2 cut(s)	6, 357
AsuC2I	CCSGG	1 cut(s)	272
AsuHPI	GGTGA	3 cut(s)	73, 736, 874
AvaII	GGWCC	2 cut(s)	6, 357
BaeGI	GKGCMC	1 cut(s)	318
BanI	GGYRCC	1 cut(s)	950
BanII	GRGCYC	1 cut(s)	557
Bbv12I	GWGCWC	2 cut(s)	115, 318
BbvI	GCAGC	2 cut(s)	469, 821
BccI	CCATC	5 cut(s)	17, 142, 499, 853, 917
BceAI	ACGGC	3 cut(s)	389, 585, 834
BcgI	CGANNNNNNTGC	2 cut(s)	363, 397
BciT130I	CCWGG	3 cut(s)	214, 819, 955
BcnI	CCSGG	1 cut(s)	272
BcoDI	GTCTC	1 cut(s)	626
BcuI	ACTAGT	1 cut(s)	377
BfaI	CTAG	1 cut(s)	378
BfmI	CTRYAG	1 cut(s)	897
BfoI	RGCGCY	2 cut(s)	309, 954
BfrI	CTTAAG	1 cut(s)	884
BglII	AGATCT	1 cut(s)	645
BisI	GCNGC	4 cut(s)	469, 472, 483, 810
BlsI	GCNGC	4 cut(s)	470, 473, 484, 811
Bme1390I	CCNGG	4 cut(s)	214, 272, 819, 955
Bme18I	GGWCC	2 cut(s)	6, 357
BmgT120I	GGNCC	2 cut(s)	6, 357
BmiI	GGNNCC	5 cut(s)	223, 276, 359, 690, 952
BmrFI	CCNGG	4 cut(s)	214, 272, 819, 955
BpmI	CTGGAG	1 cut(s)	235
BpuMI	CCSGG	1 cut(s)	272
BsaAI	YACGTR	1 cut(s)	84
BsaHI	GRCGYC	1 cut(s)	951
BsaI	GGTCTC	1 cut(s)	626
BsaJI	CCNNGG	5 cut(s)	186, 469, 817, 818, 954
BsaWI	WCCGGW	1 cut(s)	697
BsaXI	ACNNNNNCTCC	4 cut(s)	390, 420, 708, 738
Bsc4I	CCNNNNNNNGG	5 cut(s)	192, 391, 562, 596, 912
Bse118I	RCCGGY	1 cut(s)	302
Bse1I	ACTGG	4 cut(s)	295, 469, 481, 674
BseAI	TCCGGA	1 cut(s)	697
BseBI	CCWGG	3 cut(s)	214, 819, 955
BseDI	CCNNGG	5 cut(s)	186, 469, 817, 818, 954
BseGI	GGATG	4 cut(s)	134, 510, 895, 973
BseLI	CCNNNNNNNGG	5 cut(s)	192, 391, 562, 596, 912
BseMII	CTCAG	1 cut(s)	463
BseNI	ACTGG	4 cut(s)	295, 469, 481, 674
BseRI	GAGGAG	1 cut(s)	208
BseSI	GKGCMC	1 cut(s)	318
BseXI	GCAGC	2 cut(s)	469, 821
Bsh1236I	CGCG	1 cut(s)	471
BshFI	GGCC	2 cut(s)	404, 468
BshNI	GGYRCC	1 cut(s)	950
BsiHKAI	GWGCWC	2 cut(s)	115, 318
BsiSI	CCGG	3 cut(s)	272, 303, 698
BslFI	GGGAC	4 cut(s)	212, 573, 835, 887
BslI	CCNNNNNNNGG	5 cut(s)	192, 391, 562, 596, 912
BsmAI	GTCTC	1 cut(s)	626
BsmFI	GGGAC	4 cut(s)	212, 573, 835, 887
BsmI	GAATGC	1 cut(s)	622
BsnI	GGCC	2 cut(s)	404, 468
Bso31I	GGTCTC	1 cut(s)	626
Bsp1286I	GDGCHC	3 cut(s)	115, 318, 557
Bsp13I	TCCGGA	1 cut(s)	697
Bsp143I	GATC	4 cut(s)	388, 421, 645, 670
BspACI	CCGC	6 cut(s)	158, 469, 471, 620, 652, 847
BspANI	GGCC	2 cut(s)	404, 468
BspCNI	CTCAG	1 cut(s)	462
BspEI	TCCGGA	1 cut(s)	697
BspFNI	CGCG	1 cut(s)	471
BspLI	GGNNCC	5 cut(s)	223, 276, 359, 690, 952
BspPI	GGATC	1 cut(s)	665
BspT107I	GGYRCC	1 cut(s)	950
BspTI	CTTAAG	1 cut(s)	884
BspTNI	GGTCTC	1 cut(s)	626
BsrFI	RCCGGY	1 cut(s)	302
BsrI	ACTGG	4 cut(s)	295, 469, 481, 674
BssAI	RCCGGY	1 cut(s)	302
BssECI	CCNNGG	5 cut(s)	186, 469, 817, 818, 954
BssMI	GATC	4 cut(s)	388, 421, 645, 670
BssNI	GRCGYC	1 cut(s)	951
BssT1I	CCWWGG	1 cut(s)	186
Bst2UI	CCWGG	3 cut(s)	214, 819, 955
Bst4CI	ACNGT	7 cut(s)	320, 431, 739, 775, 829, 856, 881
BstACI	GRCGYC	1 cut(s)	951
BstAFI	CTTAAG	1 cut(s)	884
BstBAI	YACGTR	1 cut(s)	84
BstC8I	GCNNGC	1 cut(s)	304
BstDEI	CTNAG	1 cut(s)	449
BstDSI	CCRYGG	1 cut(s)	469
BstENI	CCTNNNNNAGG	1 cut(s)	190
BstF5I	GGATG	4 cut(s)	134, 510, 895, 973
BstFNI	CGCG	1 cut(s)	471
BstH2I	RGCGCY	2 cut(s)	309, 954
BstHHI	GCGC	3 cut(s)	308, 814, 953
BstKTI	GATC	4 cut(s)	391, 424, 648, 673
BstMAI	GTCTC	1 cut(s)	626
BstMBI	GATC	4 cut(s)	388, 421, 645, 670
BstMWI	GCNNNNNNNGC	3 cut(s)	155, 242, 488
BstNI	CCWGG	3 cut(s)	214, 819, 955
BstSCI	CCNGG	4 cut(s)	212, 270, 817, 953
BstSFI	CTRYAG	1 cut(s)	897
BstSLI	GKGCMC	1 cut(s)	318
BstUI	CGCG	1 cut(s)	471
BstV1I	GCAGC	2 cut(s)	469, 821
BstX2I	RGATCY	2 cut(s)	645, 670
BstYI	RGATCY	2 cut(s)	645, 670
BsuRI	GGCC	2 cut(s)	404, 468
BtgI	CCRYGG	1 cut(s)	469
BtgZI	GCGATG	1 cut(s)	139
BtsCI	GGATG	4 cut(s)	134, 510, 895, 973
BtsIMutI	CAGTG	3 cut(s)	474, 681, 861
Cac8I	GCNNGC	1 cut(s)	304
CfoI	GCGC	3 cut(s)	308, 814, 953
Cfr10I	RCCGGY	1 cut(s)	302
Cfr13I	GGNCC	2 cut(s)	6, 357
Cfr42I	CCGCGG	1 cut(s)	472
Csp6I	GTAC	3 cut(s)	85, 776, 852
CviAII	CATG	5 cut(s)	558, 637, 779, 800, 931
CviQI	GTAC	3 cut(s)	85, 776, 852
DdeI	CTNAG	1 cut(s)	449
DinI	GGCGCC	1 cut(s)	952
DpnI	GATC	4 cut(s)	390, 423, 647, 672
DpnII	GATC	4 cut(s)	388, 421, 645, 670
DraI	TTTAAA	2 cut(s)	498, 921
EaeI	YGGCCR	1 cut(s)	466
EciI	GGCGGA	1 cut(s)	836
Eco130I	CCWWGG	1 cut(s)	186
Eco24I	GRGCYC	1 cut(s)	557
Eco31I	GGTCTC	1 cut(s)	626
Eco32I	GATATC	2 cut(s)	17, 844
Eco47I	GGWCC	2 cut(s)	6, 357
Eco57I	CTGAAG	2 cut(s)	258, 983
EcoNI	CCTNNNNNAGG	1 cut(s)	190
EcoO109I	RGGNCCY	1 cut(s)	357
EcoRII	CCWGG	3 cut(s)	212, 817, 953
EcoRV	GATATC	2 cut(s)	17, 844
EcoT14I	CCWWGG	1 cut(s)	186
EcoT38I	GRGCYC	1 cut(s)	557
EgeI	GGCGCC	1 cut(s)	952
EheI	GGCGCC	1 cut(s)	952
ErhI	CCWWGG	1 cut(s)	186
FaeI	CATG	5 cut(s)	561, 640, 782, 803, 934
FaqI	GGGAC	4 cut(s)	212, 573, 835, 887
FatI	CATG	5 cut(s)	557, 636, 778, 799, 930
FauI	CCCGC	1 cut(s)	645
FblI	GTMKAC	1 cut(s)	249
Fnu4HI	GCNGC	4 cut(s)	469, 472, 483, 810
FokI	GGATG	4 cut(s)	121, 517, 902, 980
FriOI	GRGCYC	1 cut(s)	557
Fsp4HI	GCNGC	4 cut(s)	469, 472, 483, 810
FspBI	CTAG	1 cut(s)	378
GlaI	GCGC	3 cut(s)	307, 813, 952
GluI	GCNGC	4 cut(s)	469, 472, 483, 810
GsuI	CTGGAG	1 cut(s)	235
HaeII	RGCGCY	2 cut(s)	309, 954
HaeIII	GGCC	2 cut(s)	404, 468
HapII	CCGG	3 cut(s)	272, 303, 698
HhaI	GCGC	3 cut(s)	308, 814, 953
Hin1I	GRCGYC	1 cut(s)	951
Hin1II	CATG	5 cut(s)	561, 640, 782, 803, 934
Hin6I	GCGC	3 cut(s)	306, 812, 951
HinP1I	GCGC	3 cut(s)	306, 812, 951
HincII	GTYRAC	2 cut(s)	250, 443
HindII	GTYRAC	2 cut(s)	250, 443
HinfI	GANTC	1 cut(s)	760
HpaII	CCGG	3 cut(s)	272, 303, 698
HphI	GGTGA	3 cut(s)	73, 736, 874
Hpy166II	GTNNAC	5 cut(s)	250, 316, 443, 793, 865
Hpy188I	TCNGA	5 cut(s)	14, 166, 207, 645, 688
Hpy188III	TCNNGA	2 cut(s)	698, 913
Hpy8I	GTNNAC	5 cut(s)	250, 316, 443, 793, 865
HpyAV	CCTTC	3 cut(s)	167, 702, 958
HpyCH4III	ACNGT	7 cut(s)	320, 431, 739, 775, 829, 856, 881
HpyCH4IV	ACGT	3 cut(s)	83, 153, 351
HpyCH4V	TGCA	7 cut(s)	29, 316, 485, 491, 519, 799, 809
HpyF10VI	GCNNNNNNNGC	3 cut(s)	155, 242, 488
HpyF3I	CTNAG	1 cut(s)	449
HpySE526I	ACGT	3 cut(s)	83, 153, 351
Hsp92I	GRCGYC	1 cut(s)	951
Hsp92II	CATG	5 cut(s)	561, 640, 782, 803, 934
HspAI	GCGC	3 cut(s)	306, 812, 951
KasI	GGCGCC	1 cut(s)	950
Kpn2I	TCCGGA	1 cut(s)	697
KroI	GCCGGC	1 cut(s)	302
KroNI	GCCGGC	1 cut(s)	304
KspI	CCGCGG	1 cut(s)	472
Kzo9I	GATC	4 cut(s)	388, 421, 645, 670
LmnI	GCTCC	4 cut(s)	110, 280, 688, 958
Lsp1109I	GCAGC	2 cut(s)	469, 821
MaeI	CTAG	1 cut(s)	378
MaeII	ACGT	3 cut(s)	83, 153, 351
MaeIII	GTNAC	4 cut(s)	431, 565, 655, 676
MalI	GATC	4 cut(s)	390, 423, 647, 672
MboI	GATC	4 cut(s)	388, 421, 645, 670
MboII	GAAGA	2 cut(s)	769, 851
MflI	RGATCY	2 cut(s)	645, 670
MhlI	GDGCHC	3 cut(s)	115, 318, 557
MluCI	AATT	7 cut(s)	70, 282, 492, 529, 682, 729, 742
Mly113I	GGCGCC	1 cut(s)	951
MmeI	TCCRAC	4 cut(s)	210, 376, 577, 765
MroI	TCCGGA	1 cut(s)	697
MroNI	GCCGGC	1 cut(s)	302
MseI	TTAA	6 cut(s)	342, 497, 612, 732, 885, 920
MspA1I	CMGCKG	1 cut(s)	471
MspCI	CTTAAG	1 cut(s)	884
MspI	CCGG	3 cut(s)	272, 303, 698
MspR9I	CCNGG	4 cut(s)	214, 272, 819, 955
Mva1269I	GAATGC	1 cut(s)	622
MvaI	CCWGG	3 cut(s)	214, 819, 955
MvnI	CGCG	1 cut(s)	471
MwoI	GCNNNNNNNGC	3 cut(s)	155, 242, 488
NaeI	GCCGGC	1 cut(s)	304
NarI	GGCGCC	1 cut(s)	951
NciI	CCSGG	1 cut(s)	272
NdeII	GATC	4 cut(s)	388, 421, 645, 670
NgoMIV	GCCGGC	1 cut(s)	302
NlaIII	CATG	5 cut(s)	561, 640, 782, 803, 934
NlaIV	GGNNCC	5 cut(s)	223, 276, 359, 690, 952
NmuCI	GTSAC	3 cut(s)	565, 655, 676
PasI	CCCWGGG	1 cut(s)	818
PctI	GAATGC	1 cut(s)	622
PdiI	GCCGGC	1 cut(s)	304
PfeI	GAWTC	1 cut(s)	760
PkrI	GCNGC	4 cut(s)	470, 473, 484, 811
PluTI	GGCGCC	1 cut(s)	954
Ppu21I	YACGTR	1 cut(s)	84
PpuMI	RGGWCCY	1 cut(s)	357
PshBI	ATTAAT	1 cut(s)	342
Psp5II	RGGWCCY	1 cut(s)	357
Psp6I	CCWGG	3 cut(s)	212, 817, 953
PspGI	CCWGG	3 cut(s)	212, 817, 953
PspN4I	GGNNCC	5 cut(s)	223, 276, 359, 690, 952
PspPI	GGNCC	2 cut(s)	6, 357
PspPPI	RGGWCCY	1 cut(s)	357
PsuI	RGATCY	2 cut(s)	645, 670
RsaI	GTAC	3 cut(s)	86, 777, 853
RsaNI	GTAC	3 cut(s)	85, 776, 852
SacII	CCGCGG	1 cut(s)	472
SalI	GTCGAC	1 cut(s)	248
SaqAI	TTAA	6 cut(s)	342, 497, 612, 732, 885, 920
SatI	GCNGC	4 cut(s)	469, 472, 483, 810
Sau3AI	GATC	4 cut(s)	388, 421, 645, 670
Sau96I	GGNCC	2 cut(s)	6, 357
ScrFI	CCNGG	4 cut(s)	214, 272, 819, 955
SduI	GDGCHC	3 cut(s)	115, 318, 557
SetI	ASST	8 cut(s)	86, 144, 156, 354, 396, 726, 792, 963
SfcI	CTRYAG	1 cut(s)	897
SfoI	GGCGCC	1 cut(s)	952
Sfr303I	CCGCGG	1 cut(s)	472
SgrBI	CCGCGG	1 cut(s)	472
SinI	GGWCC	2 cut(s)	6, 357
SmlI	CTYRAG	1 cut(s)	884
SmoI	CTYRAG	1 cut(s)	884
SpeI	ACTAGT	1 cut(s)	377
Sse9I	AATT	7 cut(s)	70, 282, 492, 529, 682, 729, 742
SsiI	CCGC	6 cut(s)	158, 469, 471, 620, 652, 847
SspDI	GGCGCC	1 cut(s)	950
SspMI	CTAG	1 cut(s)	378
StyD4I	CCNGG	4 cut(s)	212, 270, 817, 953
StyI	CCWWGG	1 cut(s)	186
TaaI	ACNGT	7 cut(s)	320, 431, 739, 775, 829, 856, 881
TaiI	ACGT	3 cut(s)	86, 156, 354
TaqI	TCGA	4 cut(s)	22, 45, 76, 249
TasI	AATT	7 cut(s)	70, 282, 492, 529, 682, 729, 742
TauI	GCSGC	2 cut(s)	471, 474
TfiI	GAWTC	1 cut(s)	760
Tru1I	TTAA	6 cut(s)	342, 497, 612, 732, 885, 920
Tru9I	TTAA	6 cut(s)	342, 497, 612, 732, 885, 920
TscAI	CASTG	3 cut(s)	481, 681, 861
TseFI	GTSAC	3 cut(s)	565, 655, 676
TseI	GCWGC	2 cut(s)	482, 809
Tsp45I	GTSAC	3 cut(s)	565, 655, 676
TspDTI	ATGAA	5 cut(s)	186, 678, 720, 906, 919
TspRI	CASTG	3 cut(s)	481, 681, 861
Vha464I	CTTAAG	1 cut(s)	884
VneI	GTGCAC	1 cut(s)	314
VpaK11BI	GGWCC	2 cut(s)	6, 357
VspI	ATTAAT	1 cut(s)	342
XagI	CCTNNNNNAGG	1 cut(s)	190
XapI	RAATTY	1 cut(s)	282
XcmI	CCANNNNNNNNNTGG	2 cut(s)	88, 392
XmiI	GTMKAC	1 cut(s)	249
XspI	CTAG	1 cut(s)	378

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.