pycom15g01790

Ribosomal RNA small subunit methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0015451
Physical Location & Seq
Reverse (-)
1035370 .. 1037859
2490 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1116 bp
ATGCTATCGCGACGGCTTCCTTTCACGGCGGTTTCTTTCTCATGGGGGCGGTCCGGCCTATCGAACTTGCTCCCCTCCCAGACGATTCAGACGGTGCAGCCGTCTCTCCACTCACTCTCCGTTTGCCCTAATAAAGCTAGCATAATCCCCTTCTGTTCTTCCAGTCCTCAAATCTCCGCCGAACTCACCGACTCAATCCCCCAACCGCCGTCGAAACAAGGTCCTATTGAACCCGGACTGTATCTGGTCGGAACTCCGATCGGAAACCTCGAAGATATCACTCTACGGGCTCTCCGAGTGTTAAAATCAGCTCATGTAATACTTTCCGAAGACACTCGGCATTCGGGGAAGTTGCTCCACCATTATAGCATCAAAACTCCCCTTCTGAGCTATCACAAATTCAATGAAGCTCAAAGGGGGCAAACAGTGTTGAAGCGGTTGAAGGAAGGTGAAATTGTGGCGCTCATAAGCGATGCTGGGATGCCGGGCATCAGTGATCCTGGTACGGACTTGGCAAAGCTATGCGTGGATGAAAACATCCCTGTCATTCCTATACCTGGGCCGTCCGCATTTGTGGCTGCTCTCTCTGCCTCTGGCCTGTCCACAGATGAGTTTACATTTGTTGGATTTCTCTCTAAACATGCTGGATCTAGAAGAGAGAGGCTGATGGTTTCAGCAAATGAGGCAGCGACTCAAATATTTTATGTACCTCCCCATAAGCTTCATCAGTTTCTTGAAGAGACTTCTTCACTTTTTGGTTTTTCAAGAAAATGTGTCATAGCTCGTGAAATTACAAAAATTCATGAAGAGTTTTGGCGGGGTACATTGGGGGAAGCTGAAGAAGCGTACTCTACTCACCAGCCAAAAGGTGAAATTACAGTATTAATTGAAGGAAAGGAAGATTCTCCGGTTGTAGCTCCTTCAGAGTCTCAGCTTGAGGATGAGTTAAGAGATTTGATCTCCAGCGGGCATAGTCTTTCCACGGCTGTCAAATTAGTGGCTGACGGGACATCAGTGCGAAGGAAAACGATATATTCAATCGCATTGAAAAAATTCGGGAAGCAACTCGGTTCACAGAGTGATGCACATCCATGCAAGCCACAGCTAGATGAGTAA

Protein Analysis

372

Amino Acids

40.36

Weight (kDa)

6.68

Isoelectric Point (pI)

49.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TP_methylase PF00590 80 - 279 2.6e-32 Tetrapyrrole (Corrin/Porphyrin) Methylases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015451)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45110
fragaria_vesca FvH4_2g39240
malus_domestica MD08G1024000.v1.1 MD15G1021600.v1.1
prunus_persica Prupe.1G373400_v2.0.a1
pyrus_communis pycom15g01790
rosa_chinensis RchiOBHm_Chr6g0301771
rosa_laevigata RLG00000011223
rosa_multiflora Rmu_sc0005454.1_g000021
rosa_roxburghii Rroxscaffold_7G00166420
rosa_rugosa Rorug06G0309500
rosa_samantha Rh6AG421400 Rh6BG428200 Rh6CG434700 Rh6DG421600
rosa_wichuraiana Rw6G036480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 10
AciI CCGC 8 cut(s) 29, 49, 177, 206, 436, 567, 817, 966
AclWI GGATC 2 cut(s) 491, 655
AcsI RAATTY 3 cut(s) 398, 798, 1052
AcuI CTGAAG 2 cut(s) 858, 906
AdeI CACNNNGTG 1 cut(s) 1079
AfaI GTAC 4 cut(s) 505, 708, 823, 848
AfiI CCNNNNNNNGG 1 cut(s) 557
AgsI TTSAA 9 cut(s) 230, 403, 433, 442, 737, 765, 890, 1038, 1048
AjnI CCWGG 2 cut(s) 499, 556
Alw26I GTCTC 3 cut(s) 108, 734, 933
AlwI GGATC 2 cut(s) 491, 655
AoxI GGCC 3 cut(s) 55, 560, 595
ApeKI GCWGC 3 cut(s) 97, 578, 686
ApoI RAATTY 3 cut(s) 398, 798, 1052
AseI ATTAAT 1 cut(s) 884
Asp700I GAANNNNTTC 1 cut(s) 1052
AspLEI GCGC 1 cut(s) 463
AspS9I GGNCC 3 cut(s) 51, 221, 560
AsuC2I CCSGG 2 cut(s) 234, 486
AsuHPI GGTGA 4 cut(s) 178, 461, 848, 881
AsuNHI GCTAGC 1 cut(s) 137
AvaII GGWCC 2 cut(s) 51, 221
BanII GRGCYC 1 cut(s) 292
BauI CACGAG 1 cut(s) 783
BbsI GAAGAC 1 cut(s) 336
BbvI GCAGC 3 cut(s) 109, 565, 698
BccI CCATC 1 cut(s) 661
BceAI ACGGC 6 cut(s) 29, 42, 85, 193, 547, 999
BciT130I CCWGG 2 cut(s) 501, 558
BcnI CCSGG 2 cut(s) 234, 486
BcoDI GTCTC 3 cut(s) 108, 734, 933
BfaI CTAG 3 cut(s) 138, 651, 1106
BfoI RGCGCY 1 cut(s) 464
BisI GCNGC 3 cut(s) 98, 579, 687
BlsI GCNGC 3 cut(s) 99, 580, 688
Bme1390I CCNGG 4 cut(s) 234, 486, 501, 558
Bme18I GGWCC 2 cut(s) 51, 221
BmgT120I GGNCC 3 cut(s) 51, 221, 560
BmrFI CCNGG 4 cut(s) 234, 486, 501, 558
BmsI GCATC 5 cut(s) 378, 463, 471, 498, 1072
BmtI GCTAGC 1 cut(s) 141
BpiI GAAGAC 1 cut(s) 336
BpmI CTGGAG 1 cut(s) 946
BpuEI CTTGAG 1 cut(s) 956
BpuMI CCSGG 2 cut(s) 234, 486
BsaBI GATNNNNATC 1 cut(s) 1086
BsaJI CCNNGG 2 cut(s) 557, 981
BsaWI WCCGGW 1 cut(s) 907
BsaXI ACNNNNNCTCC 4 cut(s) 101, 131, 276, 306
Bsc4I CCNNNNNNNGG 1 cut(s) 557
Bse1I ACTGG 1 cut(s) 162
Bse8I GATNNNNATC 1 cut(s) 1086
BseBI CCWGG 2 cut(s) 501, 558
BseDI CCNNGG 2 cut(s) 557, 981
BseGI GGATG 5 cut(s) 486, 535, 537, 946, 1087
BseJI GATNNNNATC 1 cut(s) 1086
BseLI CCNNNNNNNGG 1 cut(s) 557
BseMII CTCAG 2 cut(s) 377, 944
BseNI ACTGG 1 cut(s) 162
BseXI GCAGC 3 cut(s) 109, 565, 698
BseYI CCCAGC 1 cut(s) 476
BsgI GTGCAG 1 cut(s) 116
Bsh1236I CGCG 1 cut(s) 10
Bsh1285I CGRYCG 1 cut(s) 261
BshFI GGCC 3 cut(s) 57, 562, 597
BsiEI CGRYCG 1 cut(s) 261
BsiSI CCGG 4 cut(s) 54, 234, 485, 908
BslFI GGGAC 1 cut(s) 1021
BslI CCNNNNNNNGG 1 cut(s) 557
BsmAI GTCTC 3 cut(s) 108, 734, 933
BsmBI CGTCTC 1 cut(s) 108
BsmFI GGGAC 1 cut(s) 1021
BsmI GAATGC 1 cut(s) 340
BsnI GGCC 3 cut(s) 57, 562, 597
Bsp1286I GDGCHC 1 cut(s) 292
Bsp143I GATC 4 cut(s) 258, 496, 647, 957
Bsp68I TCGCGA 1 cut(s) 10
BspACI CCGC 8 cut(s) 29, 49, 177, 206, 436, 567, 817, 966
BspANI GGCC 3 cut(s) 57, 562, 597
BspCNI CTCAG 2 cut(s) 378, 943
BspFNI CGCG 1 cut(s) 10
BspHI TCATGA 1 cut(s) 802
BspOI GCTAGC 1 cut(s) 141
BspPI GGATC 2 cut(s) 491, 655
BsrI ACTGG 1 cut(s) 162
BssECI CCNNGG 2 cut(s) 557, 981
BssMI GATC 4 cut(s) 258, 496, 647, 957
BssSI CACGAG 1 cut(s) 783
Bst2BI CACGAG 1 cut(s) 783
Bst2UI CCWGG 2 cut(s) 501, 558
Bst4CI ACNGT 4 cut(s) 94, 240, 427, 880
Bst6I CTCTTC 3 cut(s) 649, 732, 801
BstC8I GCNNGC 3 cut(s) 139, 968, 1097
BstDEI CTNAG 2 cut(s) 386, 930
BstDSI CCRYGG 1 cut(s) 981
BstF5I GGATG 5 cut(s) 486, 535, 537, 946, 1087
BstFNI CGCG 1 cut(s) 10
BstH2I RGCGCY 1 cut(s) 464
BstHHI GCGC 1 cut(s) 463
BstKTI GATC 4 cut(s) 261, 499, 650, 960
BstMAI GTCTC 3 cut(s) 108, 734, 933
BstMBI GATC 4 cut(s) 258, 496, 647, 957
BstMCI CGRYCG 1 cut(s) 261
BstMWI GCNNNNNNNGC 4 cut(s) 575, 587, 683, 842
BstNI CCWGG 2 cut(s) 501, 558
BstNSI RCATGY 1 cut(s) 644
BstSCI CCNGG 4 cut(s) 232, 484, 499, 556
BstUI CGCG 1 cut(s) 10
BstV1I GCAGC 3 cut(s) 109, 565, 698
BstV2I GAAGAC 1 cut(s) 336
BstX2I RGATCY 1 cut(s) 647
BstYI RGATCY 1 cut(s) 647
BsuRI GGCC 3 cut(s) 57, 562, 597
BtgI CCRYGG 1 cut(s) 981
BtgZI GCGATG 1 cut(s) 486
BtsCI GGATG 5 cut(s) 486, 535, 537, 946, 1087
BtsIMutI CAGTG 3 cut(s) 432, 499, 1020
BtuMI TCGCGA 1 cut(s) 10
Cac8I GCNNGC 3 cut(s) 139, 968, 1097
CciI TCATGA 1 cut(s) 802
CfoI GCGC 1 cut(s) 463
Cfr13I GGNCC 3 cut(s) 51, 221, 560
CpoI CGGWCCG 1 cut(s) 51
Csp6I GTAC 4 cut(s) 504, 707, 822, 847
CspI CGGWCCG 1 cut(s) 51
CviAII CATG 5 cut(s) 42, 314, 641, 803, 1092
CviQI GTAC 4 cut(s) 504, 707, 822, 847
DdeI CTNAG 2 cut(s) 386, 930
DpnI GATC 4 cut(s) 260, 498, 649, 959
DpnII GATC 4 cut(s) 258, 496, 647, 957
DraIII CACNNNGTG 1 cut(s) 1079
Eam1104I CTCTTC 3 cut(s) 649, 732, 801
EarI CTCTTC 3 cut(s) 649, 732, 801
EciI GGCGGA 1 cut(s) 166
Eco24I GRGCYC 1 cut(s) 292
Eco32I GATATC 1 cut(s) 277
Eco47I GGWCC 2 cut(s) 51, 221
Eco57I CTGAAG 2 cut(s) 858, 906
EcoO109I RGGNCCY 1 cut(s) 221
EcoRII CCWGG 2 cut(s) 499, 556
EcoRV GATATC 1 cut(s) 277
EcoT38I GRGCYC 1 cut(s) 292
Esp3I CGTCTC 1 cut(s) 108
FaeI CATG 5 cut(s) 45, 317, 644, 806, 1095
FaqI GGGAC 1 cut(s) 1021
FatI CATG 5 cut(s) 41, 313, 640, 802, 1091
FauI CCCGC 2 cut(s) 810, 959
Fnu4HI GCNGC 3 cut(s) 98, 579, 687
FokI GGATG 5 cut(s) 493, 524, 542, 953, 1074
FriOI GRGCYC 1 cut(s) 292
Fsp4HI GCNGC 3 cut(s) 98, 579, 687
FspBI CTAG 3 cut(s) 138, 651, 1106
GlaI GCGC 1 cut(s) 462
GluI GCNGC 3 cut(s) 98, 579, 687
GsaI CCCAGC 1 cut(s) 480
GsuI CTGGAG 1 cut(s) 946
HaeII RGCGCY 1 cut(s) 464
HaeIII GGCC 3 cut(s) 57, 562, 597
HapII CCGG 4 cut(s) 54, 234, 485, 908
HhaI GCGC 1 cut(s) 463
Hin1II CATG 5 cut(s) 45, 317, 644, 806, 1095
Hin6I GCGC 1 cut(s) 461
HinP1I GCGC 1 cut(s) 461
HindIII AAGCTT 1 cut(s) 719
HinfI GANTC 5 cut(s) 85, 191, 691, 902, 926
HpaII CCGG 4 cut(s) 54, 234, 485, 908
HphI GGTGA 4 cut(s) 178, 461, 848, 881
Hpy166II GTNNAC 3 cut(s) 603, 615, 1073
Hpy188I TCNGA 8 cut(s) 90, 251, 258, 263, 296, 328, 387, 925
Hpy188III TCNNGA 7 cut(s) 9, 651, 734, 765, 785, 803, 1057
Hpy8I GTNNAC 3 cut(s) 603, 615, 1073
Hpy99I CGWCG 2 cut(s) 15, 214
HpyAV CCTTC 7 cut(s) 160, 392, 436, 440, 884, 930, 1014
HpyCH4III ACNGT 4 cut(s) 94, 240, 427, 880
HpyCH4V TGCA 3 cut(s) 97, 1085, 1095
HpyF10VI GCNNNNNNNGC 4 cut(s) 575, 587, 683, 842
HpyF3I CTNAG 2 cut(s) 386, 930
Hsp92II CATG 5 cut(s) 45, 317, 644, 806, 1095
HspAI GCGC 1 cut(s) 461
Kzo9I GATC 4 cut(s) 258, 496, 647, 957
LmnI GCTCC 3 cut(s) 75, 360, 922
Lsp1109I GCAGC 3 cut(s) 109, 565, 698
LweI GCATC 5 cut(s) 378, 463, 471, 498, 1072
MaeI CTAG 3 cut(s) 138, 651, 1106
MalI GATC 4 cut(s) 260, 498, 649, 959
MboI GATC 4 cut(s) 258, 496, 647, 957
MboII GAAGA 9 cut(s) 150, 284, 341, 666, 738, 749, 818, 851, 911
MflI RGATCY 1 cut(s) 647
MhlI GDGCHC 1 cut(s) 292
MluCI AATT 8 cut(s) 398, 453, 789, 798, 873, 885, 992, 1052
MlyI GAGTC 3 cut(s) 185, 685, 935
MmeI TCCRAC 2 cut(s) 229, 604
MnlI CCTC 8 cut(s) 85, 177, 278, 601, 654, 676, 720, 931
MroXI GAANNNNTTC 1 cut(s) 1052
MseI TTAA 3 cut(s) 302, 884, 947
MslI CAYNNNNRTG 1 cut(s) 1090
MspA1I CMGCKG 1 cut(s) 966
MspI CCGG 4 cut(s) 54, 234, 485, 908
MspR9I CCNGG 4 cut(s) 234, 486, 501, 558
Mva1269I GAATGC 1 cut(s) 340
MvaI CCWGG 2 cut(s) 501, 558
MvnI CGCG 1 cut(s) 10
MwoI GCNNNNNNNGC 4 cut(s) 575, 587, 683, 842
NciI CCSGG 2 cut(s) 234, 486
NdeII GATC 4 cut(s) 258, 496, 647, 957
NheI GCTAGC 1 cut(s) 137
NlaIII CATG 5 cut(s) 45, 317, 644, 806, 1095
NmeAIII GCCGAG 1 cut(s) 316
NruI TCGCGA 1 cut(s) 10
NspI RCATGY 1 cut(s) 644
PagI TCATGA 1 cut(s) 802
PcsI WCGNNNNNNNCGW 3 cut(s) 98, 267, 292
PctI GAATGC 1 cut(s) 340
PdmI GAANNNNTTC 1 cut(s) 1052
PfeI GAWTC 2 cut(s) 85, 902
PkrI GCNGC 3 cut(s) 99, 580, 688
Ple19I CGATCG 1 cut(s) 261
PleI GAGTC 3 cut(s) 185, 685, 934
PpsI GAGTC 3 cut(s) 185, 685, 934
PpuMI RGGWCCY 1 cut(s) 221
PshBI ATTAAT 1 cut(s) 884
Psp5II RGGWCCY 1 cut(s) 221
Psp6I CCWGG 2 cut(s) 499, 556
PspFI CCCAGC 1 cut(s) 476
PspGI CCWGG 2 cut(s) 499, 556
PspPI GGNCC 3 cut(s) 51, 221, 560
PspPPI RGGWCCY 1 cut(s) 221
PsuI RGATCY 1 cut(s) 647
PvuI CGATCG 1 cut(s) 261
RruI TCGCGA 1 cut(s) 10
RsaI GTAC 4 cut(s) 505, 708, 823, 848
RsaNI GTAC 4 cut(s) 504, 707, 822, 847
RseI CAYNNNNRTG 1 cut(s) 1090
Rsr2I CGGWCCG 1 cut(s) 51
RsrII CGGWCCG 1 cut(s) 51
SaqAI TTAA 3 cut(s) 302, 884, 947
SatI GCNGC 3 cut(s) 98, 579, 687
Sau3AI GATC 4 cut(s) 258, 496, 647, 957
Sau96I GGNCC 3 cut(s) 51, 221, 560
SchI GAGTC 3 cut(s) 185, 685, 935
ScrFI CCNGG 4 cut(s) 234, 486, 501, 558
SduI GDGCHC 1 cut(s) 292
SfaNI GCATC 5 cut(s) 378, 463, 471, 498, 1072
SinI GGWCC 2 cut(s) 51, 221
SmiMI CAYNNNNRTG 1 cut(s) 1090
SmlI CTYRAG 1 cut(s) 935
SmoI CTYRAG 1 cut(s) 935
Sse9I AATT 8 cut(s) 398, 453, 789, 798, 873, 885, 992, 1052
SsiI CCGC 8 cut(s) 29, 49, 177, 206, 436, 567, 817, 966
SspI AATATT 1 cut(s) 699
SspMI CTAG 3 cut(s) 138, 651, 1106
StyD4I CCNGG 4 cut(s) 232, 484, 499, 556
TaaI ACNGT 4 cut(s) 94, 240, 427, 880
TaqI TCGA 3 cut(s) 62, 212, 270
TasI AATT 8 cut(s) 398, 453, 789, 798, 873, 885, 992, 1052
TfiI GAWTC 2 cut(s) 85, 902
Tru1I TTAA 3 cut(s) 302, 884, 947
Tru9I TTAA 3 cut(s) 302, 884, 947
TscAI CASTG 3 cut(s) 432, 499, 1020
TseI GCWGC 3 cut(s) 97, 578, 686
TspDTI ATGAA 5 cut(s) 420, 546, 713, 791, 819
TspGWI ACGGA 2 cut(s) 109, 521
TspRI CASTG 3 cut(s) 432, 499, 1020
VpaK11BI GGWCC 2 cut(s) 51, 221
VspI ATTAAT 1 cut(s) 884
XapI RAATTY 3 cut(s) 398, 798, 1052
XbaI TCTAGA 1 cut(s) 650
XceI RCATGY 1 cut(s) 644
XmnI GAANNNNTTC 1 cut(s) 1052
XspI CTAG 3 cut(s) 138, 651, 1106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.