RchiOBHm_Chr6g0301771

Ribosomal RNA small subunit methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0015451
Physical Location & Seq
Forward (+)
62054002 .. 62057253
3252 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27105

Sequence Viewer

Length: 1101 bp
ATGCTAGCACGCCGACTTCCGTCTACGGCGGCTGCTTTCTCACGCCGGTGGGCCGGACTGCCGAACTTGGGCCGTACTCCGTCGTCTCTGCAATCACTCCCCGTTTTCCCTAATAAAATTACCCTGATTGCCTTCGGTTCCACCGCAGCGCAAATGTCCGCAGAACACACGGACTCAATCCCCCAGGCCTCGTCAAAACAAGGTGCTCTTAAACCCGGTTTATACTTGGTCGGAACTCCGATTGGAAATCTTGAAGATATCACCTTCCGGGCTCTCCGTGTCCTGAAATCAGCCCATGTGATACTCTCAGAGGACACTAGGCACTCAGGGAAGTTGCTCCATCACTACAACATCAAAACTCCTCTTCTGAGCTATCACAAGTTCAATGAATCCCAGAGGGAACAGACGGTGTTAATGAGGTTGAAGGAAGGTGAGATCGTTGCGCTTATAAGCGATGCTGGGATGCCAGGCATTAGTGATCCTGGTACGGAACTGGCTAAACTCTGTGCGAATGAAAATATTCCTGTTATTCCTATCCCCGGACCGTCTGCTTTTGTGGCTGCTCTCTCTGCTTCAGGCCTGTCTACTGATGAGTTTACTTTTGTTGGATTCCTCTCTAAACATTCTGGAACTAGAAAAGAGAGACTAACAGTTTCTGCAAGTGAGGCAACAACACAGATATTTTATGTTCCTCCCCACAAGCTTCTTCAGTTTCTTGAAGAGACTTCTGCACTTTTCGGTGTTACAAGACAATGCGTAATAGCTCGTGAACTGACAAAAATTCATGAAGAGTTTTGGCGGGGTACATTGGGGGAAGCAGAACAATGGTTTACAACTCACCAGCCAAGAGGTGAAATTACAGTATTGATTGAAGGAAAGGAAGATATTCCGGTTGAAACTCCATCAGAGTCTCAGCTTGAGAATGAGTTGAGAGATTTGATCTCCAATGGGCATAGTCTTTCTACGGCTGTCAAATTAGTAGCTGATGGAACATCGGTGAAAAGGAAAATTATATATTCAATTGCATTGAGAAAATTCGGGAAGCAACAACTTGTTTCAGAGAGTGATTCTTCAGATCCATCAAAGCCACATCGAGAGTGA

Protein Analysis

366

Amino Acids

40.1

Weight (kDa)

7.84

Isoelectric Point (pI)

45.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TP_methylase PF00590 74 - 273 2.9e-34 Tetrapyrrole (Corrin/Porphyrin) Methylases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015451)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45110
fragaria_vesca FvH4_2g39240
malus_domestica MD08G1024000.v1.1 MD15G1021600.v1.1
prunus_persica Prupe.1G373400_v2.0.a1
pyrus_communis pycom15g01790
rosa_chinensis RchiOBHm_Chr6g0301771
rosa_laevigata RLG00000011223
rosa_multiflora Rmu_sc0005454.1_g000021
rosa_roxburghii Rroxscaffold_7G00166420
rosa_rugosa Rorug06G0309500
rosa_samantha Rh6AG421400 Rh6BG428200 Rh6CG434700 Rh6DG421600
rosa_wichuraiana Rw6G036480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 449
AccI GTMKAC 2 cut(s) 23, 584
AciI CCGC 4 cut(s) 29, 144, 159, 799
AclWI GGATC 2 cut(s) 473, 1070
AcsI RAATTY 2 cut(s) 780, 1034
AcuI CTGAAG 3 cut(s) 558, 692, 1056
AfaI GTAC 3 cut(s) 76, 487, 805
AfiI CCNNNNNNNGG 2 cut(s) 68, 539
AgsI TTSAA 7 cut(s) 254, 385, 424, 719, 872, 896, 1020
AjnI CCWGG 3 cut(s) 183, 466, 481
AleI CACNNNNGTG 1 cut(s) 46
AluBI AGCT 5 cut(s) 372, 703, 764, 916, 983
AluI AGCT 5 cut(s) 372, 703, 764, 916, 983
Alw21I GWGCWC 1 cut(s) 208
Alw26I GTCTC 4 cut(s) 90, 637, 716, 915
AlwI GGATC 2 cut(s) 473, 1070
AlwNI CAGNNNCTG 1 cut(s) 656
AoxI GGCC 4 cut(s) 51, 70, 186, 577
ApeKI GCWGC 3 cut(s) 32, 146, 560
ApoI RAATTY 2 cut(s) 780, 1034
Asp700I GAANNNNTTC 2 cut(s) 519, 885
AspLEI GCGC 2 cut(s) 151, 445
AspS9I GGNCC 3 cut(s) 51, 70, 542
AsuC2I CCSGG 3 cut(s) 216, 269, 540
AsuHPI GGTGA 5 cut(s) 253, 443, 830, 863, 1009
AsuNHI GCTAGC 1 cut(s) 4
AvaII GGWCC 1 cut(s) 542
BanII GRGCYC 1 cut(s) 274
BauI CACGAG 1 cut(s) 765
Bbv12I GWGCWC 1 cut(s) 208
BbvI GCAGC 3 cut(s) 19, 158, 547
BccI CCATC 4 cut(s) 348, 910, 980, 1087
BceAI ACGGC 3 cut(s) 42, 57, 981
BciT130I CCWGG 3 cut(s) 185, 468, 483
BcnI CCSGG 3 cut(s) 216, 269, 540
BcoDI GTCTC 4 cut(s) 90, 637, 716, 915
BfaI CTAG 3 cut(s) 5, 318, 633
BisI GCNGC 4 cut(s) 30, 33, 147, 561
BlsI GCNGC 4 cut(s) 31, 34, 148, 562
Bme1390I CCNGG 6 cut(s) 185, 216, 269, 468, 483, 540
Bme18I GGWCC 1 cut(s) 542
BmgT120I GGNCC 3 cut(s) 51, 70, 542
BmiI GGNNCC 1 cut(s) 139
BmrFI CCNGG 6 cut(s) 185, 216, 269, 468, 483, 540
BmsI GCATC 2 cut(s) 445, 453
BmtI GCTAGC 1 cut(s) 8
BoxI GACNNNNGTC 1 cut(s) 19
BpuEI CTTGAG 1 cut(s) 938
BpuMI CCSGG 3 cut(s) 216, 269, 540
BsaJI CCNNGG 2 cut(s) 183, 538
BsaWI WCCGGW 1 cut(s) 889
Bsc4I CCNNNNNNNGG 2 cut(s) 68, 539
Bse118I RCCGGY 1 cut(s) 45
Bse1I ACTGG 1 cut(s) 498
BseBI CCWGG 3 cut(s) 185, 468, 483
BseDI CCNNGG 2 cut(s) 183, 538
BseGI GGATG 1 cut(s) 468
BseLI CCNNNNNNNGG 2 cut(s) 68, 539
BseMII CTCAG 4 cut(s) 321, 339, 359, 926
BseNI ACTGG 1 cut(s) 498
BseRI GAGGAG 1 cut(s) 351
BseXI GCAGC 3 cut(s) 19, 158, 547
BseYI CCCAGC 1 cut(s) 458
BsgI GTGCAG 1 cut(s) 714
BshFI GGCC 4 cut(s) 53, 72, 188, 579
BsiHKAI GWGCWC 1 cut(s) 208
BsiSI CCGG 6 cut(s) 46, 54, 216, 268, 540, 890
BslI CCNNNNNNNGG 2 cut(s) 68, 539
BsmAI GTCTC 4 cut(s) 90, 637, 716, 915
BsmBI CGTCTC 1 cut(s) 90
BsnI GGCC 4 cut(s) 53, 72, 188, 579
Bsp1286I GDGCHC 2 cut(s) 208, 274
Bsp143I GATC 4 cut(s) 435, 478, 939, 1075
BspACI CCGC 4 cut(s) 29, 144, 159, 799
BspANI GGCC 4 cut(s) 53, 72, 188, 579
BspCNI CTCAG 4 cut(s) 320, 338, 360, 925
BspHI TCATGA 1 cut(s) 784
BspLI GGNNCC 1 cut(s) 139
BspOI GCTAGC 1 cut(s) 8
BspPI GGATC 2 cut(s) 473, 1070
BsrFI RCCGGY 1 cut(s) 45
BsrI ACTGG 1 cut(s) 498
BssAI RCCGGY 1 cut(s) 45
BssECI CCNNGG 2 cut(s) 183, 538
BssMI GATC 4 cut(s) 435, 478, 939, 1075
BssSI CACGAG 1 cut(s) 765
Bst2BI CACGAG 1 cut(s) 765
Bst2UI CCWGG 3 cut(s) 185, 468, 483
Bst4CI ACNGT 4 cut(s) 409, 546, 652, 862
Bst6I CTCTTC 3 cut(s) 369, 714, 783
BstC8I GCNNGC 2 cut(s) 6, 10
BstDEI CTNAG 4 cut(s) 307, 325, 368, 912
BstF5I GGATG 1 cut(s) 468
BstHHI GCGC 2 cut(s) 151, 445
BstKTI GATC 4 cut(s) 438, 481, 942, 1078
BstMAI GTCTC 4 cut(s) 90, 637, 716, 915
BstMBI GATC 4 cut(s) 435, 478, 939, 1075
BstMWI GCNNNNNNNGC 3 cut(s) 557, 569, 665
BstNI CCWGG 3 cut(s) 185, 468, 483
BstPAI GACNNNNGTC 1 cut(s) 19
BstSCI CCNGG 6 cut(s) 183, 214, 267, 466, 481, 538
BstV1I GCAGC 3 cut(s) 19, 158, 547
BstX2I RGATCY 1 cut(s) 1075
BstYI RGATCY 1 cut(s) 1075
BsuRI GGCC 4 cut(s) 53, 72, 188, 579
BtgZI GCGATG 1 cut(s) 468
BtsCI GGATG 1 cut(s) 468
Cac8I GCNNGC 2 cut(s) 6, 10
CaiI CAGNNNCTG 1 cut(s) 656
CciI TCATGA 1 cut(s) 784
CfoI GCGC 2 cut(s) 151, 445
Cfr10I RCCGGY 1 cut(s) 45
Cfr13I GGNCC 3 cut(s) 51, 70, 542
CpoI CGGWCCG 1 cut(s) 542
Csp6I GTAC 3 cut(s) 75, 486, 804
CspI CGGWCCG 1 cut(s) 542
CviAII CATG 2 cut(s) 296, 785
CviQI GTAC 3 cut(s) 75, 486, 804
DdeI CTNAG 4 cut(s) 307, 325, 368, 912
DpnI GATC 4 cut(s) 437, 480, 941, 1077
DpnII GATC 4 cut(s) 435, 478, 939, 1075
Eam1104I CTCTTC 3 cut(s) 369, 714, 783
EarI CTCTTC 3 cut(s) 369, 714, 783
Eco147I AGGCCT 2 cut(s) 188, 579
Eco24I GRGCYC 1 cut(s) 274
Eco32I GATATC 1 cut(s) 259
Eco47I GGWCC 1 cut(s) 542
Eco57I CTGAAG 3 cut(s) 558, 692, 1056
EcoRII CCWGG 3 cut(s) 183, 466, 481
EcoRV GATATC 1 cut(s) 259
EcoT38I GRGCYC 1 cut(s) 274
Esp3I CGTCTC 1 cut(s) 90
FaeI CATG 2 cut(s) 299, 788
FaiI YATR 8 cut(s) 223, 297, 449, 687, 786, 954, 1013, 1015
FalI AAGNNNNNCTT 2 cut(s) 192, 224
FatI CATG 2 cut(s) 295, 784
FauI CCCGC 1 cut(s) 792
FblI GTMKAC 2 cut(s) 23, 584
Fnu4HI GCNGC 4 cut(s) 30, 33, 147, 561
FokI GGATG 1 cut(s) 475
FriOI GRGCYC 1 cut(s) 274
Fsp4HI GCNGC 4 cut(s) 30, 33, 147, 561
FspBI CTAG 3 cut(s) 5, 318, 633
GlaI GCGC 2 cut(s) 150, 444
GluI GCNGC 4 cut(s) 30, 33, 147, 561
GsaI CCCAGC 1 cut(s) 462
HaeIII GGCC 4 cut(s) 53, 72, 188, 579
HapII CCGG 6 cut(s) 46, 54, 216, 268, 540, 890
HhaI GCGC 2 cut(s) 151, 445
Hin1II CATG 2 cut(s) 299, 788
Hin6I GCGC 2 cut(s) 149, 443
HinP1I GCGC 2 cut(s) 149, 443
HindIII AAGCTT 1 cut(s) 701
HinfI GANTC 5 cut(s) 173, 389, 609, 908, 1067
HpaII CCGG 6 cut(s) 46, 54, 216, 268, 540, 890
HphI GGTGA 5 cut(s) 253, 443, 830, 863, 1009
Hpy166II GTNNAC 5 cut(s) 24, 585, 597, 770, 831
Hpy188I TCNGA 7 cut(s) 233, 240, 310, 369, 907, 1060, 1075
Hpy188III TCNNGA 8 cut(s) 251, 283, 627, 716, 767, 785, 1039, 1094
Hpy8I GTNNAC 5 cut(s) 24, 585, 597, 770, 831
Hpy99I CGWCG 1 cut(s) 85
HpyAV CCTTC 5 cut(s) 142, 274, 418, 422, 866
HpyCH4III ACNGT 4 cut(s) 409, 546, 652, 862
HpyCH4V TGCA 4 cut(s) 91, 659, 731, 1025
HpyF10VI GCNNNNNNNGC 3 cut(s) 557, 569, 665
HpyF3I CTNAG 4 cut(s) 307, 325, 368, 912
Hsp92II CATG 2 cut(s) 299, 788
HspAI GCGC 2 cut(s) 149, 443
Kzo9I GATC 4 cut(s) 435, 478, 939, 1075
LmnI GCTCC 1 cut(s) 342
Lsp1109I GCAGC 3 cut(s) 19, 158, 547
LweI GCATC 2 cut(s) 445, 453
MaeI CTAG 3 cut(s) 5, 318, 633
MaeIII GTNAC 1 cut(s) 742
MalI GATC 4 cut(s) 437, 480, 941, 1077
MboI GATC 4 cut(s) 435, 478, 939, 1075
MboII GAAGA 7 cut(s) 266, 356, 698, 731, 800, 893, 1062
MfeI CAATTG 1 cut(s) 1020
MflI RGATCY 1 cut(s) 1075
MhlI GDGCHC 2 cut(s) 208, 274
MluCI AATT 7 cut(s) 117, 780, 855, 974, 1008, 1020, 1034
MlyI GAGTC 2 cut(s) 167, 917
MmeI TCCRAC 2 cut(s) 211, 586
MnlI CCTC 9 cut(s) 199, 304, 372, 390, 411, 623, 658, 702, 842
MroXI GAANNNNTTC 2 cut(s) 519, 885
MseI TTAA 2 cut(s) 210, 413
MslI CAYNNNNRTG 1 cut(s) 46
MspI CCGG 6 cut(s) 46, 54, 216, 268, 540, 890
MspR9I CCNGG 6 cut(s) 185, 216, 269, 468, 483, 540
MunI CAATTG 1 cut(s) 1020
MvaI CCWGG 3 cut(s) 185, 468, 483
MwoI GCNNNNNNNGC 3 cut(s) 557, 569, 665
NciI CCSGG 3 cut(s) 216, 269, 540
NdeII GATC 4 cut(s) 435, 478, 939, 1075
NheI GCTAGC 1 cut(s) 4
NlaIII CATG 2 cut(s) 299, 788
NlaIV GGNNCC 1 cut(s) 139
OliI CACNNNNGTG 1 cut(s) 46
PagI TCATGA 1 cut(s) 784
PceI AGGCCT 2 cut(s) 188, 579
PdmI GAANNNNTTC 2 cut(s) 519, 885
PfeI GAWTC 3 cut(s) 389, 609, 1067
PkrI GCNGC 4 cut(s) 31, 34, 148, 562
PleI GAGTC 2 cut(s) 167, 916
PpsI GAGTC 2 cut(s) 167, 916
PshAI GACNNNNGTC 1 cut(s) 19
PsiI TTATAA 1 cut(s) 449
Psp6I CCWGG 3 cut(s) 183, 466, 481
PspFI CCCAGC 1 cut(s) 458
PspGI CCWGG 3 cut(s) 183, 466, 481
PspN4I GGNNCC 1 cut(s) 139
PspPI GGNCC 3 cut(s) 51, 70, 542
PstNI CAGNNNCTG 1 cut(s) 656
PsuI RGATCY 1 cut(s) 1075
RsaI GTAC 3 cut(s) 76, 487, 805
RsaNI GTAC 3 cut(s) 75, 486, 804
RseI CAYNNNNRTG 1 cut(s) 46
Rsr2I CGGWCCG 1 cut(s) 542
RsrII CGGWCCG 1 cut(s) 542
SaqAI TTAA 2 cut(s) 210, 413
SatI GCNGC 4 cut(s) 30, 33, 147, 561
Sau3AI GATC 4 cut(s) 435, 478, 939, 1075
Sau96I GGNCC 3 cut(s) 51, 70, 542
SchI GAGTC 2 cut(s) 167, 917
ScrFI CCNGG 6 cut(s) 185, 216, 269, 468, 483, 540
SduI GDGCHC 2 cut(s) 208, 274
SfaNI GCATC 2 cut(s) 445, 453
SgrAI CRCCGGYG 1 cut(s) 45
SinI GGWCC 1 cut(s) 542
SmiMI CAYNNNNRTG 1 cut(s) 46
SmlI CTYRAG 1 cut(s) 917
SmoI CTYRAG 1 cut(s) 917
Sse9I AATT 7 cut(s) 117, 780, 855, 974, 1008, 1020, 1034
SseBI AGGCCT 2 cut(s) 188, 579
SsiI CCGC 4 cut(s) 29, 144, 159, 799
SspI AATATT 1 cut(s) 520
SspMI CTAG 3 cut(s) 5, 318, 633
StuI AGGCCT 2 cut(s) 188, 579
StyD4I CCNGG 6 cut(s) 183, 214, 267, 466, 481, 538
TaaI ACNGT 4 cut(s) 409, 546, 652, 862
TaqI TCGA 1 cut(s) 1093
TasI AATT 7 cut(s) 117, 780, 855, 974, 1008, 1020, 1034
TauI GCSGC 1 cut(s) 32
TfiI GAWTC 3 cut(s) 389, 609, 1067
Tru1I TTAA 2 cut(s) 210, 413
Tru9I TTAA 2 cut(s) 210, 413
TseI GCWGC 3 cut(s) 32, 146, 560
TspDTI ATGAA 4 cut(s) 402, 528, 773, 801
TspGWI ACGGA 5 cut(s) 9, 69, 185, 266, 503
VpaK11BI GGWCC 1 cut(s) 542
XapI RAATTY 2 cut(s) 780, 1034
XmiI GTMKAC 2 cut(s) 23, 584
XmnI GAANNNNTTC 2 cut(s) 519, 885
XspI CTAG 3 cut(s) 5, 318, 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.