Rh6CG434700

Ribosomal RNA small subunit methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0015451
Physical Location & Seq
Forward (+)
61442392 .. 61445383
2992 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG434700.1

Sequence Viewer

Length: 1107 bp
ATGCTAGCACGCCGACTTCCGTCTACGGCGGCTGGTTTCTCACGCCGGTGGGCCGGACTGCCGAACTTGGGCCGTACTCCGTCGTCTCTGCAATCACTCCCCGTTTTCCCTAATAAAATTACCCTGATTGCCTTAGGTTCCACCGCAGCGCAAATGCCCGCAGAACACACCGACTCAATCCCCCAGGGCCAGGCCTCGTCAAAACAAGGTGCTCTTAAACCCGGTTTATACTTGGTCGGAACTCCGATTGGAAATCTTGAAGATATCACCTTCCGGGCTCTCCGTGTCCTGAAATCAGCCCATGTGATACTTTCAGAGGACACTAGGCACTCAGGGAAGTTGCTCCATTACTACAACATCAAAACTCCTCTTCTGAGCTATCACAAGTTCAATGAATCCCAGAGGGAACAGACGGTGTTAATGAGGTTGAAGGAAGGTGAGATCGTTGCGCTTATAAGCGATGCTGGGATGCCAGGCATTAGTGATCCTGGTACGGAACTGGCAAAACTCTGTGCGAATGAAAATATTCCTGTTATTCCTATACCTGGACCGTCTGCTTTTGTGGCTGCTCTCTCTGCTTCAGGCCTATCTACTGATGAGTTTACTTTTGTTGGATTCCTCTCTAAACATTCTGGAACTAGAAAAGAGAGACTAACAGTTTCTGCAAGTGAGGCAACAACACAGATATTTTATGTTCCTCCCCACAAGCTTCTTCAGTTTCTTGAAGAGACTTCTTCACTTTTCGGTGTTACAAGACAATGCGTAATAGCTCGTGAACTGACAAAAATTCATGAAGAGTTTTGGCGGGGTACATTGGGGGAAGCAGAACAATGGTTTACAACTCACCAGCCAAGAGGTGAAATTACAGTATTGATTGAAGGAAAGGAAGATATTCCGGTTGAAACTCCATCAGAGTCTCAGCTTGAGAATGAGTTGAGAGATTTGATCTCCAATGGGCATAGTCTTTCTACGGCTGTCAAATTAGTAGCTGATGGAACATCGGTGAAAAGGAAAACGATATATTCAATTGCATTGAGAAAATTCGGGAAGCAACAACTTGTTTCAGAGAGTGATTCTTCAGATCCATCAAAGCCACATCGAGAGTGA

Protein Analysis

368

Amino Acids

40.27

Weight (kDa)

7.83

Isoelectric Point (pI)

47.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TP_methylase PF00590 76 - 275 5.8e-34 Tetrapyrrole (Corrin/Porphyrin) Methylases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015451)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45110
fragaria_vesca FvH4_2g39240
malus_domestica MD08G1024000.v1.1 MD15G1021600.v1.1
prunus_persica Prupe.1G373400_v2.0.a1
pyrus_communis pycom15g01790
rosa_chinensis RchiOBHm_Chr6g0301771
rosa_laevigata RLG00000011223
rosa_multiflora Rmu_sc0005454.1_g000021
rosa_roxburghii Rroxscaffold_7G00166420
rosa_rugosa Rorug06G0309500
rosa_samantha Rh6AG421400 Rh6BG428200 Rh6CG434700 Rh6DG421600
rosa_wichuraiana Rw6G036480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 455
AccI GTMKAC 1 cut(s) 23
AciI CCGC 4 cut(s) 29, 144, 159, 805
AclWI GGATC 2 cut(s) 479, 1076
AcsI RAATTY 2 cut(s) 786, 1040
AcuI CTGAAG 3 cut(s) 564, 698, 1062
AfaI GTAC 3 cut(s) 76, 493, 811
AfiI CCNNNNNNNGG 3 cut(s) 68, 190, 545
AgsI TTSAA 7 cut(s) 260, 391, 430, 725, 878, 902, 1026
AjnI CCWGG 5 cut(s) 183, 189, 472, 487, 544
AleI CACNNNNGTG 1 cut(s) 46
AluBI AGCT 5 cut(s) 378, 709, 770, 922, 989
AluI AGCT 5 cut(s) 378, 709, 770, 922, 989
Alw21I GWGCWC 1 cut(s) 214
Alw26I GTCTC 4 cut(s) 90, 643, 722, 921
AlwI GGATC 2 cut(s) 479, 1076
AlwNI CAGNNNCTG 1 cut(s) 662
AoxI GGCC 5 cut(s) 51, 70, 187, 192, 583
ApeKI GCWGC 2 cut(s) 146, 566
ApoI RAATTY 2 cut(s) 786, 1040
Asp700I GAANNNNTTC 2 cut(s) 525, 891
AspLEI GCGC 2 cut(s) 151, 451
AspS9I GGNCC 4 cut(s) 51, 70, 187, 548
AsuC2I CCSGG 2 cut(s) 222, 275
AsuHPI GGTGA 5 cut(s) 259, 449, 836, 869, 1015
AsuNHI GCTAGC 1 cut(s) 4
AvaII GGWCC 1 cut(s) 548
AxyI CCTNAGG 1 cut(s) 133
BanII GRGCYC 1 cut(s) 280
BauI CACGAG 1 cut(s) 771
Bbv12I GWGCWC 1 cut(s) 214
BbvI GCAGC 2 cut(s) 158, 553
BccI CCATC 3 cut(s) 916, 986, 1093
BceAI ACGGC 3 cut(s) 42, 57, 987
BciT130I CCWGG 5 cut(s) 185, 191, 474, 489, 546
BcnI CCSGG 2 cut(s) 222, 275
BcoDI GTCTC 4 cut(s) 90, 643, 722, 921
BfaI CTAG 3 cut(s) 5, 324, 639
BisI GCNGC 3 cut(s) 30, 147, 567
BlsI GCNGC 3 cut(s) 31, 148, 568
Bme1390I CCNGG 7 cut(s) 185, 191, 222, 275, 474, 489, 546
Bme18I GGWCC 1 cut(s) 548
BmgT120I GGNCC 4 cut(s) 51, 70, 187, 548
BmiI GGNNCC 1 cut(s) 139
BmrFI CCNGG 7 cut(s) 185, 191, 222, 275, 474, 489, 546
BmsI GCATC 2 cut(s) 451, 459
BmtI GCTAGC 1 cut(s) 8
BoxI GACNNNNGTC 1 cut(s) 19
BpuEI CTTGAG 1 cut(s) 944
BpuMI CCSGG 2 cut(s) 222, 275
BsaJI CCNNGG 2 cut(s) 183, 184
BsaWI WCCGGW 1 cut(s) 895
Bsc4I CCNNNNNNNGG 3 cut(s) 68, 190, 545
Bse118I RCCGGY 1 cut(s) 45
Bse1I ACTGG 1 cut(s) 504
Bse21I CCTNAGG 1 cut(s) 133
BseBI CCWGG 5 cut(s) 185, 191, 474, 489, 546
BseDI CCNNGG 2 cut(s) 183, 184
BseGI GGATG 1 cut(s) 474
BseLI CCNNNNNNNGG 3 cut(s) 68, 190, 545
BseMII CTCAG 3 cut(s) 345, 365, 932
BseNI ACTGG 1 cut(s) 504
BseRI GAGGAG 1 cut(s) 357
BseXI GCAGC 2 cut(s) 158, 553
BseYI CCCAGC 1 cut(s) 464
BshFI GGCC 5 cut(s) 53, 72, 189, 194, 585
BsiHKAI GWGCWC 1 cut(s) 214
BsiSI CCGG 5 cut(s) 46, 54, 222, 274, 896
BslI CCNNNNNNNGG 3 cut(s) 68, 190, 545
BsmAI GTCTC 4 cut(s) 90, 643, 722, 921
BsmBI CGTCTC 1 cut(s) 90
BsnI GGCC 5 cut(s) 53, 72, 189, 194, 585
Bsp1286I GDGCHC 2 cut(s) 214, 280
Bsp143I GATC 4 cut(s) 441, 484, 945, 1081
BspACI CCGC 4 cut(s) 29, 144, 159, 805
BspANI GGCC 5 cut(s) 53, 72, 189, 194, 585
BspCNI CTCAG 3 cut(s) 344, 366, 931
BspHI TCATGA 1 cut(s) 790
BspLI GGNNCC 1 cut(s) 139
BspOI GCTAGC 1 cut(s) 8
BspPI GGATC 2 cut(s) 479, 1076
BsrFI RCCGGY 1 cut(s) 45
BsrI ACTGG 1 cut(s) 504
BssAI RCCGGY 1 cut(s) 45
BssECI CCNNGG 2 cut(s) 183, 184
BssMI GATC 4 cut(s) 441, 484, 945, 1081
BssSI CACGAG 1 cut(s) 771
Bst2BI CACGAG 1 cut(s) 771
Bst2UI CCWGG 5 cut(s) 185, 191, 474, 489, 546
Bst4CI ACNGT 4 cut(s) 415, 552, 658, 868
Bst6I CTCTTC 3 cut(s) 375, 720, 789
BstC8I GCNNGC 3 cut(s) 6, 10, 159
BstDEI CTNAG 4 cut(s) 133, 331, 374, 918
BstF5I GGATG 1 cut(s) 474
BstHHI GCGC 2 cut(s) 151, 451
BstKTI GATC 4 cut(s) 444, 487, 948, 1084
BstMAI GTCTC 4 cut(s) 90, 643, 722, 921
BstMBI GATC 4 cut(s) 441, 484, 945, 1081
BstMWI GCNNNNNNNGC 3 cut(s) 563, 575, 671
BstNI CCWGG 5 cut(s) 185, 191, 474, 489, 546
BstPAI GACNNNNGTC 1 cut(s) 19
BstSCI CCNGG 7 cut(s) 183, 189, 220, 273, 472, 487, 544
BstV1I GCAGC 2 cut(s) 158, 553
BstX2I RGATCY 1 cut(s) 1081
BstYI RGATCY 1 cut(s) 1081
Bsu36I CCTNAGG 1 cut(s) 133
BsuRI GGCC 5 cut(s) 53, 72, 189, 194, 585
BtgZI GCGATG 1 cut(s) 474
BtsCI GGATG 1 cut(s) 474
Cac8I GCNNGC 3 cut(s) 6, 10, 159
CaiI CAGNNNCTG 1 cut(s) 662
CciI TCATGA 1 cut(s) 790
CfoI GCGC 2 cut(s) 151, 451
Cfr10I RCCGGY 1 cut(s) 45
Cfr13I GGNCC 4 cut(s) 51, 70, 187, 548
Csp6I GTAC 3 cut(s) 75, 492, 810
CviAII CATG 2 cut(s) 302, 791
CviQI GTAC 3 cut(s) 75, 492, 810
DdeI CTNAG 4 cut(s) 133, 331, 374, 918
DpnI GATC 4 cut(s) 443, 486, 947, 1083
DpnII GATC 4 cut(s) 441, 484, 945, 1081
Eam1104I CTCTTC 3 cut(s) 375, 720, 789
EarI CTCTTC 3 cut(s) 375, 720, 789
Eco147I AGGCCT 2 cut(s) 194, 585
Eco24I GRGCYC 1 cut(s) 280
Eco32I GATATC 1 cut(s) 265
Eco47I GGWCC 1 cut(s) 548
Eco57I CTGAAG 3 cut(s) 564, 698, 1062
Eco81I CCTNAGG 1 cut(s) 133
EcoRII CCWGG 5 cut(s) 183, 189, 472, 487, 544
EcoRV GATATC 1 cut(s) 265
EcoT38I GRGCYC 1 cut(s) 280
Esp3I CGTCTC 1 cut(s) 90
FaeI CATG 2 cut(s) 305, 794
FaiI YATR 8 cut(s) 229, 303, 455, 542, 693, 792, 960, 1021
FalI AAGNNNNNCTT 2 cut(s) 198, 230
FatI CATG 2 cut(s) 301, 790
FauI CCCGC 2 cut(s) 166, 798
FblI GTMKAC 1 cut(s) 23
Fnu4HI GCNGC 3 cut(s) 30, 147, 567
FokI GGATG 1 cut(s) 481
FriOI GRGCYC 1 cut(s) 280
Fsp4HI GCNGC 3 cut(s) 30, 147, 567
FspBI CTAG 3 cut(s) 5, 324, 639
GlaI GCGC 2 cut(s) 150, 450
GluI GCNGC 3 cut(s) 30, 147, 567
GsaI CCCAGC 1 cut(s) 468
HaeIII GGCC 5 cut(s) 53, 72, 189, 194, 585
HapII CCGG 5 cut(s) 46, 54, 222, 274, 896
HhaI GCGC 2 cut(s) 151, 451
Hin1II CATG 2 cut(s) 305, 794
Hin6I GCGC 2 cut(s) 149, 449
HinP1I GCGC 2 cut(s) 149, 449
HindIII AAGCTT 1 cut(s) 707
HinfI GANTC 5 cut(s) 173, 395, 615, 914, 1073
HpaII CCGG 5 cut(s) 46, 54, 222, 274, 896
HphI GGTGA 5 cut(s) 259, 449, 836, 869, 1015
Hpy166II GTNNAC 4 cut(s) 24, 603, 776, 837
Hpy188I TCNGA 7 cut(s) 239, 246, 316, 375, 913, 1066, 1081
Hpy188III TCNNGA 8 cut(s) 257, 289, 633, 722, 773, 791, 1045, 1100
Hpy8I GTNNAC 4 cut(s) 24, 603, 776, 837
Hpy99I CGWCG 1 cut(s) 85
HpyAV CCTTC 4 cut(s) 280, 424, 428, 872
HpyCH4III ACNGT 4 cut(s) 415, 552, 658, 868
HpyCH4V TGCA 3 cut(s) 91, 665, 1031
HpyF10VI GCNNNNNNNGC 3 cut(s) 563, 575, 671
HpyF3I CTNAG 4 cut(s) 133, 331, 374, 918
Hsp92II CATG 2 cut(s) 305, 794
HspAI GCGC 2 cut(s) 149, 449
Kzo9I GATC 4 cut(s) 441, 484, 945, 1081
LmnI GCTCC 1 cut(s) 348
Lsp1109I GCAGC 2 cut(s) 158, 553
LweI GCATC 2 cut(s) 451, 459
MaeI CTAG 3 cut(s) 5, 324, 639
MaeIII GTNAC 1 cut(s) 748
MalI GATC 4 cut(s) 443, 486, 947, 1083
MboI GATC 4 cut(s) 441, 484, 945, 1081
MboII GAAGA 8 cut(s) 272, 362, 704, 726, 737, 806, 899, 1068
MfeI CAATTG 1 cut(s) 1026
MflI RGATCY 1 cut(s) 1081
MhlI GDGCHC 2 cut(s) 214, 280
MluCI AATT 6 cut(s) 117, 786, 861, 980, 1026, 1040
MlyI GAGTC 2 cut(s) 167, 923
MmeI TCCRAC 2 cut(s) 217, 592
MnlI CCTC 9 cut(s) 205, 310, 378, 396, 417, 629, 664, 708, 848
MroXI GAANNNNTTC 2 cut(s) 525, 891
MseI TTAA 2 cut(s) 216, 419
MslI CAYNNNNRTG 1 cut(s) 46
MspI CCGG 5 cut(s) 46, 54, 222, 274, 896
MspR9I CCNGG 7 cut(s) 185, 191, 222, 275, 474, 489, 546
MunI CAATTG 1 cut(s) 1026
MvaI CCWGG 5 cut(s) 185, 191, 474, 489, 546
MwoI GCNNNNNNNGC 3 cut(s) 563, 575, 671
NciI CCSGG 2 cut(s) 222, 275
NdeII GATC 4 cut(s) 441, 484, 945, 1081
NheI GCTAGC 1 cut(s) 4
NlaIII CATG 2 cut(s) 305, 794
NlaIV GGNNCC 1 cut(s) 139
OliI CACNNNNGTG 1 cut(s) 46
PagI TCATGA 1 cut(s) 790
PasI CCCWGGG 1 cut(s) 184
PceI AGGCCT 2 cut(s) 194, 585
PdmI GAANNNNTTC 2 cut(s) 525, 891
PfeI GAWTC 3 cut(s) 395, 615, 1073
PkrI GCNGC 3 cut(s) 31, 148, 568
PleI GAGTC 2 cut(s) 167, 922
PpsI GAGTC 2 cut(s) 167, 922
PshAI GACNNNNGTC 1 cut(s) 19
PsiI TTATAA 1 cut(s) 455
Psp6I CCWGG 5 cut(s) 183, 189, 472, 487, 544
PspFI CCCAGC 1 cut(s) 464
PspGI CCWGG 5 cut(s) 183, 189, 472, 487, 544
PspN4I GGNNCC 1 cut(s) 139
PspPI GGNCC 4 cut(s) 51, 70, 187, 548
PstNI CAGNNNCTG 1 cut(s) 662
PsuI RGATCY 1 cut(s) 1081
RsaI GTAC 3 cut(s) 76, 493, 811
RsaNI GTAC 3 cut(s) 75, 492, 810
RseI CAYNNNNRTG 1 cut(s) 46
SaqAI TTAA 2 cut(s) 216, 419
SatI GCNGC 3 cut(s) 30, 147, 567
Sau3AI GATC 4 cut(s) 441, 484, 945, 1081
Sau96I GGNCC 4 cut(s) 51, 70, 187, 548
SchI GAGTC 2 cut(s) 167, 923
ScrFI CCNGG 7 cut(s) 185, 191, 222, 275, 474, 489, 546
SduI GDGCHC 2 cut(s) 214, 280
SfaNI GCATC 2 cut(s) 451, 459
SgrAI CRCCGGYG 1 cut(s) 45
SinI GGWCC 1 cut(s) 548
SmiMI CAYNNNNRTG 1 cut(s) 46
SmlI CTYRAG 1 cut(s) 923
SmoI CTYRAG 1 cut(s) 923
Sse9I AATT 6 cut(s) 117, 786, 861, 980, 1026, 1040
SseBI AGGCCT 2 cut(s) 194, 585
SsiI CCGC 4 cut(s) 29, 144, 159, 805
SspI AATATT 1 cut(s) 526
SspMI CTAG 3 cut(s) 5, 324, 639
StuI AGGCCT 2 cut(s) 194, 585
StyD4I CCNGG 7 cut(s) 183, 189, 220, 273, 472, 487, 544
TaaI ACNGT 4 cut(s) 415, 552, 658, 868
TaqI TCGA 1 cut(s) 1099
TasI AATT 6 cut(s) 117, 786, 861, 980, 1026, 1040
TauI GCSGC 1 cut(s) 32
TfiI GAWTC 3 cut(s) 395, 615, 1073
Tru1I TTAA 2 cut(s) 216, 419
Tru9I TTAA 2 cut(s) 216, 419
TseI GCWGC 2 cut(s) 146, 566
TspDTI ATGAA 4 cut(s) 408, 534, 779, 807
TspGWI ACGGA 4 cut(s) 9, 69, 272, 509
VpaK11BI GGWCC 1 cut(s) 548
XapI RAATTY 2 cut(s) 786, 1040
XmiI GTMKAC 1 cut(s) 23
XmnI GAANNNNTTC 2 cut(s) 525, 891
XspI CTAG 3 cut(s) 5, 324, 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.