Rh6AG421400

Ribosomal RNA small subunit methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0015451
Physical Location & Seq
Forward (+)
61453061 .. 61455938
2878 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG421400.1

Sequence Viewer

Length: 948 bp
ATGTCCGCAGAACACACCAACTCAATCCCCCAGGCCTCGTCAAAACGAGGTGCTCTTAAACCCGGTTTATACTTGGTCGGAACTCCGATTGGAAATCTTGAAGATATCACCTTCCGGGCTCTCCGTGTCCTGAAATCAGCCCATGTGATACTTTCAGAGGACACTAGGCACTCAGGGAAGTTGCTCCATCACTACAACATCAAAACTCCTCTTCTGAGCTATCACAAGTTCAATGAATCCCAGAGGGAACAGACGGTGTTAATGAGGTTGAAGGAAGGTGAGATCGTTGCGCTTATAAGCGATGCTGGGATGCCAGGCATTAGTGATCCTGGTTCGGAACTGGCAAAACTCTGTGCGAATCAAAATATTCCTGTTATTCCTATACCTGGACCGTCTGCTTTTGTGGCTGCTCTCTCTGCTTCAGGCCTGTCTACTGATGAGTTTACTTTTGTTGGATTCCTCTCTAAACATTCTGGAACTAGAAAAGAGAGACTAACAGTTTCTGCAAGTGAGGCAACAACACAGATATTTTATGTTCCTCCCCACAAGCTTCTTCAGTTTCTTGAAGAGACTTCTTCACTTTTCGGTGTTACAAGACAATGCGTAATAGCTCGTGAACTGACAAAAATTCATGAAGAGTTTTGGCGGGGTACATTGGGGGAAGCAGAACAATGGTTTACAACTCACCAGCCAAGAGGTGAAATTACAGTATTGATTGAAGGAAAGGAAGATATTCCGGTTGAAACTCCATCAGAATCTCAGCTTGAGAATGATTTGAGAGATTTGATCTCCAATGGGCATAGTCTTTCTACGGCTGTCAAATTAGTAGCTGATGGAACATCGGTGAAAAGGAAAACGATATATTCAATTGCATTGAGAAAATTCGGGAAGCAACAACTTGTTTCAGAGAGTGATTCTTCAGATCCATCAAAGCCACATCGAGAGTGA

Protein Analysis

315

Amino Acids

34.68

Weight (kDa)

6.65

Isoelectric Point (pI)

38.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TP_methylase PF00590 23 - 222 2e-34 Tetrapyrrole (Corrin/Porphyrin) Methylases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015451)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45110
fragaria_vesca FvH4_2g39240
malus_domestica MD08G1024000.v1.1 MD15G1021600.v1.1
prunus_persica Prupe.1G373400_v2.0.a1
pyrus_communis pycom15g01790
rosa_chinensis RchiOBHm_Chr6g0301771
rosa_laevigata RLG00000011223
rosa_multiflora Rmu_sc0005454.1_g000021
rosa_roxburghii Rroxscaffold_7G00166420
rosa_rugosa Rorug06G0309500
rosa_samantha Rh6AG421400 Rh6BG428200 Rh6CG434700 Rh6DG421600
rosa_wichuraiana Rw6G036480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 296
AccI GTMKAC 1 cut(s) 431
AciI CCGC 2 cut(s) 6, 646
AclWI GGATC 2 cut(s) 320, 917
AcsI RAATTY 2 cut(s) 627, 881
AcuI CTGAAG 3 cut(s) 405, 539, 903
AfaI GTAC 1 cut(s) 652
AfiI CCNNNNNNNGG 1 cut(s) 386
AgsI TTSAA 7 cut(s) 101, 232, 271, 566, 719, 743, 867
AjnI CCWGG 4 cut(s) 30, 313, 328, 385
AluBI AGCT 5 cut(s) 219, 550, 611, 763, 830
AluI AGCT 5 cut(s) 219, 550, 611, 763, 830
Alw21I GWGCWC 1 cut(s) 55
Alw26I GTCTC 2 cut(s) 484, 563
AlwI GGATC 2 cut(s) 320, 917
AlwNI CAGNNNCTG 1 cut(s) 503
AoxI GGCC 2 cut(s) 33, 424
ApeKI GCWGC 1 cut(s) 407
ApoI RAATTY 2 cut(s) 627, 881
Asp700I GAANNNNTTC 1 cut(s) 732
AspLEI GCGC 1 cut(s) 292
AspS9I GGNCC 1 cut(s) 389
AsuC2I CCSGG 2 cut(s) 63, 116
AsuHPI GGTGA 5 cut(s) 100, 290, 677, 710, 856
AvaII GGWCC 1 cut(s) 389
BanII GRGCYC 1 cut(s) 121
BauI CACGAG 1 cut(s) 612
Bbv12I GWGCWC 1 cut(s) 55
BbvI GCAGC 1 cut(s) 394
BccI CCATC 4 cut(s) 195, 757, 827, 934
BceAI ACGGC 1 cut(s) 828
BciT130I CCWGG 4 cut(s) 32, 315, 330, 387
BcnI CCSGG 2 cut(s) 63, 116
BcoDI GTCTC 2 cut(s) 484, 563
BfaI CTAG 2 cut(s) 165, 480
BisI GCNGC 1 cut(s) 408
BlsI GCNGC 1 cut(s) 409
Bme1390I CCNGG 6 cut(s) 32, 63, 116, 315, 330, 387
Bme18I GGWCC 1 cut(s) 389
BmgT120I GGNCC 1 cut(s) 389
BmrFI CCNGG 6 cut(s) 32, 63, 116, 315, 330, 387
BmsI GCATC 2 cut(s) 292, 300
BpuEI CTTGAG 1 cut(s) 785
BpuMI CCSGG 2 cut(s) 63, 116
BsaJI CCNNGG 1 cut(s) 30
BsaWI WCCGGW 1 cut(s) 736
Bsc4I CCNNNNNNNGG 1 cut(s) 386
Bse1I ACTGG 1 cut(s) 345
BseBI CCWGG 4 cut(s) 32, 315, 330, 387
BseDI CCNNGG 1 cut(s) 30
BseGI GGATG 1 cut(s) 315
BseLI CCNNNNNNNGG 1 cut(s) 386
BseMII CTCAG 3 cut(s) 186, 206, 773
BseNI ACTGG 1 cut(s) 345
BseRI GAGGAG 1 cut(s) 198
BseXI GCAGC 1 cut(s) 394
BseYI CCCAGC 1 cut(s) 305
BshFI GGCC 2 cut(s) 35, 426
BsiHKAI GWGCWC 1 cut(s) 55
BsiSI CCGG 3 cut(s) 63, 115, 737
BslI CCNNNNNNNGG 1 cut(s) 386
BsmAI GTCTC 2 cut(s) 484, 563
BsnI GGCC 2 cut(s) 35, 426
Bsp1286I GDGCHC 2 cut(s) 55, 121
Bsp143I GATC 4 cut(s) 282, 325, 786, 922
BspACI CCGC 2 cut(s) 6, 646
BspANI GGCC 2 cut(s) 35, 426
BspCNI CTCAG 3 cut(s) 185, 207, 772
BspHI TCATGA 1 cut(s) 631
BspPI GGATC 2 cut(s) 320, 917
BsrI ACTGG 1 cut(s) 345
BssECI CCNNGG 1 cut(s) 30
BssMI GATC 4 cut(s) 282, 325, 786, 922
BssSI CACGAG 1 cut(s) 612
Bst2BI CACGAG 1 cut(s) 612
Bst2UI CCWGG 4 cut(s) 32, 315, 330, 387
Bst4CI ACNGT 4 cut(s) 256, 393, 499, 709
Bst6I CTCTTC 3 cut(s) 216, 561, 630
BstDEI CTNAG 3 cut(s) 172, 215, 759
BstF5I GGATG 1 cut(s) 315
BstHHI GCGC 1 cut(s) 292
BstKTI GATC 4 cut(s) 285, 328, 789, 925
BstMAI GTCTC 2 cut(s) 484, 563
BstMBI GATC 4 cut(s) 282, 325, 786, 922
BstMWI GCNNNNNNNGC 3 cut(s) 404, 416, 512
BstNI CCWGG 4 cut(s) 32, 315, 330, 387
BstSCI CCNGG 6 cut(s) 30, 61, 114, 313, 328, 385
BstV1I GCAGC 1 cut(s) 394
BstX2I RGATCY 1 cut(s) 922
BstYI RGATCY 1 cut(s) 922
BsuRI GGCC 2 cut(s) 35, 426
BtgZI GCGATG 1 cut(s) 315
BtsCI GGATG 1 cut(s) 315
CaiI CAGNNNCTG 1 cut(s) 503
CciI TCATGA 1 cut(s) 631
CfoI GCGC 1 cut(s) 292
Cfr13I GGNCC 1 cut(s) 389
Csp6I GTAC 1 cut(s) 651
CviAII CATG 2 cut(s) 143, 632
CviQI GTAC 1 cut(s) 651
DdeI CTNAG 3 cut(s) 172, 215, 759
DpnI GATC 4 cut(s) 284, 327, 788, 924
DpnII GATC 4 cut(s) 282, 325, 786, 922
Eam1104I CTCTTC 3 cut(s) 216, 561, 630
EarI CTCTTC 3 cut(s) 216, 561, 630
Eco147I AGGCCT 2 cut(s) 35, 426
Eco24I GRGCYC 1 cut(s) 121
Eco32I GATATC 1 cut(s) 106
Eco47I GGWCC 1 cut(s) 389
Eco57I CTGAAG 3 cut(s) 405, 539, 903
EcoRII CCWGG 4 cut(s) 30, 313, 328, 385
EcoRV GATATC 1 cut(s) 106
EcoT38I GRGCYC 1 cut(s) 121
FaeI CATG 2 cut(s) 146, 635
FaiI YATR 8 cut(s) 70, 144, 296, 383, 534, 633, 801, 862
FatI CATG 2 cut(s) 142, 631
FauI CCCGC 1 cut(s) 639
FblI GTMKAC 1 cut(s) 431
Fnu4HI GCNGC 1 cut(s) 408
FokI GGATG 1 cut(s) 322
FriOI GRGCYC 1 cut(s) 121
Fsp4HI GCNGC 1 cut(s) 408
FspBI CTAG 2 cut(s) 165, 480
GlaI GCGC 1 cut(s) 291
GluI GCNGC 1 cut(s) 408
GsaI CCCAGC 1 cut(s) 309
HaeIII GGCC 2 cut(s) 35, 426
HapII CCGG 3 cut(s) 63, 115, 737
HhaI GCGC 1 cut(s) 292
Hin1II CATG 2 cut(s) 146, 635
Hin6I GCGC 1 cut(s) 290
HinP1I GCGC 1 cut(s) 290
HindIII AAGCTT 1 cut(s) 548
HinfI GANTC 5 cut(s) 236, 358, 456, 755, 914
HpaII CCGG 3 cut(s) 63, 115, 737
HphI GGTGA 5 cut(s) 100, 290, 677, 710, 856
Hpy166II GTNNAC 4 cut(s) 432, 444, 617, 678
Hpy188I TCNGA 8 cut(s) 80, 87, 157, 216, 337, 754, 907, 922
Hpy188III TCNNGA 8 cut(s) 98, 130, 474, 563, 614, 632, 886, 941
Hpy8I GTNNAC 4 cut(s) 432, 444, 617, 678
HpyAV CCTTC 4 cut(s) 121, 265, 269, 713
HpyCH4III ACNGT 4 cut(s) 256, 393, 499, 709
HpyCH4V TGCA 2 cut(s) 506, 872
HpyF10VI GCNNNNNNNGC 3 cut(s) 404, 416, 512
HpyF3I CTNAG 3 cut(s) 172, 215, 759
Hsp92II CATG 2 cut(s) 146, 635
HspAI GCGC 1 cut(s) 290
Kzo9I GATC 4 cut(s) 282, 325, 786, 922
LmnI GCTCC 1 cut(s) 189
Lsp1109I GCAGC 1 cut(s) 394
LweI GCATC 2 cut(s) 292, 300
MaeI CTAG 2 cut(s) 165, 480
MaeIII GTNAC 1 cut(s) 589
MalI GATC 4 cut(s) 284, 327, 788, 924
MboI GATC 4 cut(s) 282, 325, 786, 922
MboII GAAGA 8 cut(s) 113, 203, 545, 567, 578, 647, 740, 909
MfeI CAATTG 1 cut(s) 867
MflI RGATCY 1 cut(s) 922
MhlI GDGCHC 2 cut(s) 55, 121
MluCI AATT 5 cut(s) 627, 702, 821, 867, 881
MmeI TCCRAC 2 cut(s) 58, 433
MroXI GAANNNNTTC 1 cut(s) 732
MseI TTAA 2 cut(s) 57, 260
MspI CCGG 3 cut(s) 63, 115, 737
MspR9I CCNGG 6 cut(s) 32, 63, 116, 315, 330, 387
MunI CAATTG 1 cut(s) 867
MvaI CCWGG 4 cut(s) 32, 315, 330, 387
MwoI GCNNNNNNNGC 3 cut(s) 404, 416, 512
NciI CCSGG 2 cut(s) 63, 116
NdeII GATC 4 cut(s) 282, 325, 786, 922
NlaIII CATG 2 cut(s) 146, 635
PagI TCATGA 1 cut(s) 631
PceI AGGCCT 2 cut(s) 35, 426
PdmI GAANNNNTTC 1 cut(s) 732
PfeI GAWTC 5 cut(s) 236, 358, 456, 755, 914
PkrI GCNGC 1 cut(s) 409
PsiI TTATAA 1 cut(s) 296
Psp6I CCWGG 4 cut(s) 30, 313, 328, 385
PspFI CCCAGC 1 cut(s) 305
PspGI CCWGG 4 cut(s) 30, 313, 328, 385
PspPI GGNCC 1 cut(s) 389
PstNI CAGNNNCTG 1 cut(s) 503
PsuI RGATCY 1 cut(s) 922
RsaI GTAC 1 cut(s) 652
RsaNI GTAC 1 cut(s) 651
SaqAI TTAA 2 cut(s) 57, 260
SatI GCNGC 1 cut(s) 408
Sau3AI GATC 4 cut(s) 282, 325, 786, 922
Sau96I GGNCC 1 cut(s) 389
ScrFI CCNGG 6 cut(s) 32, 63, 116, 315, 330, 387
SduI GDGCHC 2 cut(s) 55, 121
SfaNI GCATC 2 cut(s) 292, 300
SinI GGWCC 1 cut(s) 389
SmlI CTYRAG 1 cut(s) 764
SmoI CTYRAG 1 cut(s) 764
Sse9I AATT 5 cut(s) 627, 702, 821, 867, 881
SseBI AGGCCT 2 cut(s) 35, 426
SsiI CCGC 2 cut(s) 6, 646
SspI AATATT 1 cut(s) 367
SspMI CTAG 2 cut(s) 165, 480
StuI AGGCCT 2 cut(s) 35, 426
StyD4I CCNGG 6 cut(s) 30, 61, 114, 313, 328, 385
TaaI ACNGT 4 cut(s) 256, 393, 499, 709
TaqI TCGA 1 cut(s) 940
TasI AATT 5 cut(s) 627, 702, 821, 867, 881
TfiI GAWTC 5 cut(s) 236, 358, 456, 755, 914
Tru1I TTAA 2 cut(s) 57, 260
Tru9I TTAA 2 cut(s) 57, 260
TseI GCWGC 1 cut(s) 407
TspDTI ATGAA 3 cut(s) 249, 620, 648
TspGWI ACGGA 1 cut(s) 113
VpaK11BI GGWCC 1 cut(s) 389
XapI RAATTY 2 cut(s) 627, 881
XmiI GTMKAC 1 cut(s) 431
XmnI GAANNNNTTC 1 cut(s) 732
XspI CTAG 2 cut(s) 165, 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.