pycom15g02320

Cytosolic purine 5'-nucleotidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0003145
Physical Location & Seq
Reverse (-)
1365571 .. 1368264
2694 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1098 bp
ATGTTTGCCCAACTGGTTGACTTTAAAGACAAAAATTCGGGAAAATTTCCAGATAGTGTTGATTACGCTGTTATGTACAAGGATGTTCGAGCTGCTGTTGATTTGTGCCACCGTGATGGAACTTTAAAGCAAATGGTGGCAAATGACCCTCAGCGGTACATTAATGAAGATACCTCCATAGTTCCTATGCTCAAAATGCTGAGAGATTCCGGCCGTGCTACATTCCTGGTGACAAACAGTTTATGGGACTATACAAACATTGTAATGAATTTCCTTTGTGAGTCGCGCACAGTGGATGGTAGTAGAAAATGCAATTTCGACTGGCTTAAATACTTCGATGTTGTCATCACTGGCAGTGCAAAGCCAAGCTTCTTTCATGATGGTAACCGTGCTAATCTGTTTGAGGTGGATCTCGAGTCGGGGATGCTACATAATACTGATAATGGTTCTCCTATGGCTCAGGTTGGAAGTTCTTCACCTGCACCTACATTATCATTGAACGGACTGAAGAAGGGTTGCAGAGTTTTTCAGGGAGGCAGCGTTGGGCATCTTCATAAACTGCTTTCAATTGAGTCAAGTTCACAGGTTCTTTATGTTGGTGACCATATATACGGAGATATTTTGCGCAGCAAAAAGGTTCTTGGGTGGAGAACAATGCTTGTTGTTCCAGAGCTAGAAAGGGAGGTTGAACTCCTATGGGAATTGAGAGATACCCGCAAGCAACTTCAATTATTGAGGAATGAGCGTGATCTGATTGAAGACAAAATACATCATCTAAAGTGGTCTCTCAAATATGACGATATCAGTGCTGAGGAGAAGCAAAAATTATCTTCTGAATACAATGCGTTGGAGTTCCAAAGAGACCAAGTGCGGTTCATCCATCAACAGTCTCAACGTGAATGTCACCAAATGTTTCACAAGATTTGGGGACAACTCATGAAGACTGGCTATCAGAACTCTCGCTTTGCCCATCAGGTTGAAAGATTTGCCTGCCTTTATGCTAGCCAAGTTTCCAACTTGAGCCTCTATTCTCCTGACAAATACTACAGGCCGAGTGAAGACTACATGCCCCATGAATTCGATATTCTTCCCCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
5_nucleotid

Protein Analysis

366

Amino Acids

42.3

Weight (kDa)

6.5

Isoelectric Point (pI)

46.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
5_nucleotid PF05761 1 - 360 9.5e-115 5' nucleotidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 487
Acc16I TGCGCA 1 cut(s) 626
Acc36I ACCTGC 1 cut(s) 487
AccII CGCG 1 cut(s) 286
AciI CCGC 3 cut(s) 154, 715, 871
AclWI GGATC 1 cut(s) 417
AcoI YGGCCR 1 cut(s) 211
AcsI RAATTY 4 cut(s) 34, 44, 268, 1076
AcuI CTGAAG 1 cut(s) 527
AfaI GTAC 2 cut(s) 77, 158
AgsI TTSAA 6 cut(s) 499, 567, 689, 728, 758, 980
AjnI CCWGG 1 cut(s) 225
AluBI AGCT 3 cut(s) 92, 369, 673
AluI AGCT 3 cut(s) 92, 369, 673
Alw26I GTCTC 3 cut(s) 789, 855, 894
AlwI GGATC 1 cut(s) 417
Ama87I CYCGRG 1 cut(s) 413
AoxI GGCC 2 cut(s) 211, 1049
ApeKI GCWGC 3 cut(s) 92, 537, 627
ApoI RAATTY 4 cut(s) 34, 44, 268, 1076
AseI ATTAAT 1 cut(s) 162
Asp700I GAANNNNTTC 1 cut(s) 472
AspLEI GCGC 2 cut(s) 288, 627
AsuHPI GGTGA 4 cut(s) 241, 468, 611, 896
AsuNHI GCTAGC 1 cut(s) 1001
AvaI CYCGRG 1 cut(s) 413
BarI GAAGNNNNNNTAC 2 cut(s) 750, 782
BbsI GAAGAC 3 cut(s) 765, 947, 1065
BbvCI CCTCAGC 2 cut(s) 150, 810
BbvI GCAGC 3 cut(s) 79, 549, 639
BccI CCATC 5 cut(s) 110, 290, 374, 888, 978
BceAI ACGGC 1 cut(s) 198
BcgI CGANNNNNNTGC 2 cut(s) 788, 822
BciT130I CCWGG 1 cut(s) 227
BcoDI GTCTC 3 cut(s) 789, 855, 894
BfaI CTAG 2 cut(s) 674, 1002
BfmI CTRYAG 1 cut(s) 1045
BfuAI ACCTGC 1 cut(s) 487
BisI GCNGC 3 cut(s) 93, 538, 628
BlsI GCNGC 3 cut(s) 94, 539, 629
Bme1390I CCNGG 1 cut(s) 227
BmeT110I CYCGRG 1 cut(s) 413
BmrFI CCNGG 1 cut(s) 227
BmsI GCATC 2 cut(s) 414, 556
BmtI GCTAGC 1 cut(s) 1005
BpiI GAAGAC 3 cut(s) 765, 947, 1065
Bpu10I CCTNAGC 3 cut(s) 150, 459, 810
BpuEI CTTGAG 1 cut(s) 1039
BsaI GGTCTC 2 cut(s) 789, 855
BsaXI ACNNNNNCTCC 2 cut(s) 525, 555
Bse1I ACTGG 4 cut(s) 18, 326, 355, 949
BseBI CCWGG 1 cut(s) 227
BseGI GGATG 4 cut(s) 88, 301, 429, 876
BseMII CTCAG 4 cut(s) 164, 191, 473, 801
BseNI ACTGG 4 cut(s) 18, 326, 355, 949
BseRI GAGGAG 1 cut(s) 827
BseX3I CGGCCG 1 cut(s) 211
BseXI GCAGC 3 cut(s) 79, 549, 639
BsgI GTGCAG 1 cut(s) 465
Bsh1236I CGCG 1 cut(s) 286
Bsh1285I CGRYCG 1 cut(s) 214
BshFI GGCC 2 cut(s) 213, 1051
BsiEI CGRYCG 1 cut(s) 214
BsiHKCI CYCGRG 1 cut(s) 413
BsiSI CCGG 1 cut(s) 210
BslFI GGGAC 2 cut(s) 260, 942
BsmAI GTCTC 3 cut(s) 789, 855, 894
BsmFI GGGAC 2 cut(s) 260, 942
BsnI GGCC 2 cut(s) 213, 1051
Bso31I GGTCTC 2 cut(s) 789, 855
BsoBI CYCGRG 1 cut(s) 413
Bsp1407I TGTACA 1 cut(s) 75
Bsp143I GATC 2 cut(s) 409, 748
BspACI CCGC 3 cut(s) 154, 715, 871
BspANI GGCC 2 cut(s) 213, 1051
BspCNI CTCAG 4 cut(s) 163, 192, 472, 802
BspFNI CGCG 1 cut(s) 286
BspHI TCATGA 2 cut(s) 376, 936
BspMI ACCTGC 1 cut(s) 487
BspOI GCTAGC 1 cut(s) 1005
BspPI GGATC 1 cut(s) 417
BspTNI GGTCTC 2 cut(s) 789, 855
BsrGI TGTACA 1 cut(s) 75
BsrI ACTGG 4 cut(s) 18, 326, 355, 949
BssMI GATC 2 cut(s) 409, 748
Bst2UI CCWGG 1 cut(s) 227
Bst4CI ACNGT 5 cut(s) 113, 239, 292, 389, 888
BstAUI TGTACA 1 cut(s) 75
BstC8I GCNNGC 3 cut(s) 719, 991, 1003
BstDEI CTNAG 4 cut(s) 150, 200, 459, 810
BstEII GGTNACC 2 cut(s) 383, 599
BstF5I GGATG 4 cut(s) 88, 301, 429, 876
BstFNI CGCG 1 cut(s) 286
BstHHI GCGC 2 cut(s) 288, 627
BstKTI GATC 2 cut(s) 412, 751
BstMAI GTCTC 3 cut(s) 789, 855, 894
BstMBI GATC 2 cut(s) 409, 748
BstMCI CGRYCG 1 cut(s) 214
BstMWI GCNNNNNNNGC 1 cut(s) 196
BstNI CCWGG 1 cut(s) 227
BstNSI RCATGY 1 cut(s) 1069
BstPI GGTNACC 2 cut(s) 383, 599
BstSCI CCNGG 1 cut(s) 225
BstSFI CTRYAG 1 cut(s) 1045
BstUI CGCG 1 cut(s) 286
BstV1I GCAGC 3 cut(s) 79, 549, 639
BstV2I GAAGAC 3 cut(s) 765, 947, 1065
BstX2I RGATCY 1 cut(s) 409
BstXI CCANNNNNNTGG 1 cut(s) 116
BstYI RGATCY 1 cut(s) 409
BstZI CGGCCG 1 cut(s) 211
BsuRI GGCC 2 cut(s) 213, 1051
BtsCI GGATG 4 cut(s) 88, 301, 429, 876
BtsI GCAGTG 1 cut(s) 361
BtsIMutI CAGTG 4 cut(s) 297, 348, 361, 811
BveI ACCTGC 1 cut(s) 487
Cac8I GCNNGC 3 cut(s) 719, 991, 1003
CciI TCATGA 2 cut(s) 376, 936
CfoI GCGC 2 cut(s) 288, 627
Csp6I GTAC 2 cut(s) 76, 157
CviAII CATG 4 cut(s) 377, 937, 1066, 1073
CviQI GTAC 2 cut(s) 76, 157
DdeI CTNAG 4 cut(s) 150, 200, 459, 810
DpnI GATC 2 cut(s) 411, 750
DpnII GATC 2 cut(s) 409, 748
DraI TTTAAA 2 cut(s) 25, 126
EaeI YGGCCR 1 cut(s) 211
EagI CGGCCG 1 cut(s) 211
EclXI CGGCCG 1 cut(s) 211
Eco31I GGTCTC 2 cut(s) 789, 855
Eco32I GATATC 1 cut(s) 802
Eco52I CGGCCG 1 cut(s) 211
Eco57I CTGAAG 1 cut(s) 527
Eco88I CYCGRG 1 cut(s) 413
Eco91I GGTNACC 2 cut(s) 383, 599
EcoO65I GGTNACC 2 cut(s) 383, 599
EcoRI GAATTC 1 cut(s) 1076
EcoRII CCWGG 1 cut(s) 225
EcoRV GATATC 1 cut(s) 802
FaeI CATG 4 cut(s) 380, 940, 1069, 1076
FalI AAGNNNNNCTT 2 cut(s) 353, 385
FaqI GGGAC 2 cut(s) 260, 942
FatI CATG 4 cut(s) 376, 936, 1065, 1072
FauI CCCGC 1 cut(s) 722
Fnu4HI GCNGC 3 cut(s) 93, 538, 628
FokI GGATG 4 cut(s) 95, 308, 436, 863
Fsp4HI GCNGC 3 cut(s) 93, 538, 628
FspBI CTAG 2 cut(s) 674, 1002
FspI TGCGCA 1 cut(s) 626
GlaI GCGC 2 cut(s) 287, 626
GluI GCNGC 3 cut(s) 93, 538, 628
HaeIII GGCC 2 cut(s) 213, 1051
HapII CCGG 1 cut(s) 210
HhaI GCGC 2 cut(s) 288, 627
Hin1II CATG 4 cut(s) 380, 940, 1069, 1076
Hin6I GCGC 2 cut(s) 286, 625
HinP1I GCGC 2 cut(s) 286, 625
HincII GTYRAC 1 cut(s) 19
HindII GTYRAC 1 cut(s) 19
HindIII AAGCTT 1 cut(s) 367
HinfI GANTC 4 cut(s) 206, 281, 416, 572
HpaII CCGG 1 cut(s) 210
HphI GGTGA 4 cut(s) 241, 468, 611, 896
Hpy166II GTNNAC 2 cut(s) 19, 581
Hpy188I TCNGA 3 cut(s) 753, 835, 954
Hpy188III TCNNGA 7 cut(s) 39, 50, 377, 413, 668, 937, 1034
Hpy8I GTNNAC 2 cut(s) 19, 581
HpyAV CCTTC 1 cut(s) 505
HpyCH4III ACNGT 5 cut(s) 113, 239, 292, 389, 888
HpyCH4IV ACGT 1 cut(s) 895
HpyCH4V TGCA 4 cut(s) 312, 359, 482, 519
HpyF10VI GCNNNNNNNGC 1 cut(s) 196
HpyF3I CTNAG 4 cut(s) 150, 200, 459, 810
HpySE526I ACGT 1 cut(s) 895
Hsp92II CATG 4 cut(s) 380, 940, 1069, 1076
HspAI GCGC 2 cut(s) 286, 625
Kzo9I GATC 2 cut(s) 409, 748
Lsp1109I GCAGC 3 cut(s) 79, 549, 639
LweI GCATC 2 cut(s) 414, 556
MaeI CTAG 2 cut(s) 674, 1002
MaeII ACGT 1 cut(s) 895
MaeIII GTNAC 4 cut(s) 229, 383, 599, 902
MalI GATC 2 cut(s) 411, 750
MboI GATC 2 cut(s) 409, 748
MboII GAAGA 9 cut(s) 179, 465, 520, 542, 770, 822, 952, 1070, 1079
MfeI CAATTG 1 cut(s) 567
MflI RGATCY 1 cut(s) 409
MluCI AATT 9 cut(s) 34, 44, 268, 313, 567, 701, 728, 824, 1076
MlyI GAGTC 3 cut(s) 290, 425, 581
MmeI TCCRAC 3 cut(s) 445, 828, 1038
MnlI CCTC 8 cut(s) 159, 184, 397, 527, 676, 729, 805, 1034
MroXI GAANNNNTTC 1 cut(s) 472
MseI TTAA 4 cut(s) 24, 125, 162, 327
MslI CAYNNNNRTG 2 cut(s) 114, 263
MspA1I CMGCKG 1 cut(s) 154
MspI CCGG 1 cut(s) 210
MspR9I CCNGG 1 cut(s) 227
MunI CAATTG 1 cut(s) 567
MvaI CCWGG 1 cut(s) 227
MvnI CGCG 1 cut(s) 286
MwoI GCNNNNNNNGC 1 cut(s) 196
NdeII GATC 2 cut(s) 409, 748
NheI GCTAGC 1 cut(s) 1001
NlaIII CATG 4 cut(s) 380, 940, 1069, 1076
NmeAIII GCCGAG 1 cut(s) 1077
NmuCI GTSAC 3 cut(s) 229, 599, 902
NsbI TGCGCA 1 cut(s) 626
NspI RCATGY 1 cut(s) 1069
PaeR7I CTCGAG 1 cut(s) 413
PagI TCATGA 2 cut(s) 376, 936
PaqCI CACCTGC 1 cut(s) 487
PdmI GAANNNNTTC 1 cut(s) 472
PfeI GAWTC 1 cut(s) 206
PkrI GCNGC 3 cut(s) 94, 539, 629
PleI GAGTC 3 cut(s) 289, 424, 580
PpsI GAGTC 3 cut(s) 289, 424, 580
PshBI ATTAAT 1 cut(s) 162
Psp6I CCWGG 1 cut(s) 225
PspEI GGTNACC 2 cut(s) 383, 599
PspGI CCWGG 1 cut(s) 225
PsuI RGATCY 1 cut(s) 409
RsaI GTAC 2 cut(s) 77, 158
RsaNI GTAC 2 cut(s) 76, 157
RseI CAYNNNNRTG 2 cut(s) 114, 263
SaqAI TTAA 4 cut(s) 24, 125, 162, 327
SatI GCNGC 3 cut(s) 93, 538, 628
Sau3AI GATC 2 cut(s) 409, 748
SchI GAGTC 3 cut(s) 290, 425, 581
ScrFI CCNGG 1 cut(s) 227
SfaNI GCATC 2 cut(s) 414, 556
SfcI CTRYAG 1 cut(s) 1045
Sfr274I CTCGAG 1 cut(s) 413
SlaI CTCGAG 1 cut(s) 413
SmiMI CAYNNNNRTG 2 cut(s) 114, 263
SmlI CTYRAG 2 cut(s) 413, 1018
SmoI CTYRAG 2 cut(s) 413, 1018
Sse9I AATT 9 cut(s) 34, 44, 268, 313, 567, 701, 728, 824, 1076
SsiI CCGC 3 cut(s) 154, 715, 871
SspMI CTAG 2 cut(s) 674, 1002
StyD4I CCNGG 1 cut(s) 225
TaaI ACNGT 5 cut(s) 113, 239, 292, 389, 888
TaiI ACGT 1 cut(s) 898
TaqI TCGA 5 cut(s) 88, 318, 336, 414, 1080
TasI AATT 9 cut(s) 34, 44, 268, 313, 567, 701, 728, 824, 1076
TatI WGTACW 1 cut(s) 75
TfiI GAWTC 1 cut(s) 206
Tru1I TTAA 4 cut(s) 24, 125, 162, 327
Tru9I TTAA 4 cut(s) 24, 125, 162, 327
TscAI CASTG 4 cut(s) 297, 355, 361, 811
TseFI GTSAC 3 cut(s) 229, 599, 902
TseI GCWGC 3 cut(s) 92, 537, 627
Tsp45I GTSAC 3 cut(s) 229, 599, 902
TspDTI ATGAA 7 cut(s) 180, 281, 365, 542, 865, 953, 1089
TspGWI ACGGA 2 cut(s) 516, 627
TspRI CASTG 4 cut(s) 297, 355, 361, 811
VspI ATTAAT 1 cut(s) 162
XapI RAATTY 4 cut(s) 34, 44, 268, 1076
XceI RCATGY 1 cut(s) 1069
XhoI CTCGAG 1 cut(s) 413
XmnI GAANNNNTTC 1 cut(s) 472
XspI CTAG 2 cut(s) 674, 1002
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.