pycom15g02830

Leishmanolysin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0010930
Physical Location & Seq
Forward (+)
1751129 .. 1756912
5784 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2439 bp
ATGGAGGTGATGATGCGGCGTAGTCCATGTCCTTCTCCCAGATTCGCCGTCGTCGTCGTCAAGGTTATATTGCTGTTTATATGGTTGCAAGCTAGTAATGCATCACTCCAAGAAAACACTCTGCAAGGGCAAAGCCCAGAATTGTTATCCGATAGCATTGTTTCACATTCCTGCATTCATGACCAGATAATCAAACAGCGCAGGCAACCTGGTCGCAAAGTGTACACTGTTACCCCGCAGGCGTATGAGGGGTCTGGTGTATCAAAACCCCTTCACCAAAAGGGTAGGGCGTTACTTGGAATTTCCAAGTACTCTGTACAGCAGAAGGATATTAAAAGGCCTATTAGAATATATTTAAATTATGATGCTGTTGGTCACTCTCCTGATAGAGATTGTCGTAATGTTGGCGATGTTGTGAAGCTTGGGGAGCCTCCTGTTATTTCTCTTCCTGGCTCTCCTTCTTGTAATCCTCGTGGTGATCCTCCAATTTCTGGTGATTGCTGGTATAACTGCACTTTGGATGATATAGCTGGGAAGGACAAAAGACAACGCCTCCGCAAGGCTCTAGGGCAAACAGCAGACTGGTTCAAGAGAGCCCTAGCTGTTGAACCTGTGAGGGGGAACTTGCGGTTGAGTGGGTATTCAGCATGCGGCCAGGATGGAGGTGTACAACTACCACAGGGTGTTGCTGAAGCAGACCTGGTTCTTCTGGTGACAACAAGACCTACCACTGGGAACACCCTTGCATGGGCAGTGGCTTGCGAACGTACAATCAAAGATCGATCAATGGGGCCGTGCAATTGCTGGGATGAGAGGAAAAGAAGGCGTAGTCAGGTTACAGAACAAATAATGGATGAAAAGCTTGGGCGTATGGTGACTCGTGTGGTTCTTCCACGTGTAGTCATGCATTCAAGATATCATTATATGGCATTCTCTGAGAATTTCACGGGTTTAGAGCTAGAAGATGGTGGAGGGCGTGGCACATCAGGGTCACACTGGGAGAAAAGACTTCTAATGAATGAAATTATGACGGGGTCAGTGGATACAAGATCCGTGGTTTCAAAAATGACACTTGCTTTATTAGAAGATAGTGGATGGTACCAGGCTAATTATAGTATGGCGGACACTCTTGATTGGGGCCGCAACCAAGGAACTGAATTTGTTACTTCCCCTTGCAATTTCTGGAAGGGGGCATATCACTGCAACACAACCCAATCGTCAGGATGTACATACAACAGAGAAGCAGAAGGTTACTGTCCAATTGTAAGTTACAGTGGAGACCTACCCCAGTGGGCACGCTATTTTCCCCAGGCTAACAAGGGTGGGCAGTCCCCACTGGCTGATTATTGCACCTATTTCGTTGCCTACTCTGATGGATCATGTACTGACACTAACAGCGCTCGACCACCTGACAGAATGTTGGGTGAAGTACGAGGAAGTAACTCTAGGTGTATGGCTTCATCCTTGGTACGTACAGGGTTTGTACGGGGTTCTATGACCCAAGGAAATGGATGTTATCAGCACAGATGTATTAACAATTCGTTAGAGGTTGCTGTGGATGGTATGTGGAAAGTATGTCCTGAAGCTGGTGGACCCCTTCAGTTCCCAGGATTTAATGGGGAATTGTTATGCCCGGCGTATGATGAACTCTGTAGTAAAGCCCTAGTTCCTGCAAATGGACAATGTCCAAAGTCATGCAATTTCAATGGAGACTGTGTTGAAGGAAGATGTCACTGTTTTCTAGGGTTTCATGGTTCTGATTGTAGTAAACGCACCTGCCCAAGCAACTGTAATGGACGTGGAAGCTGCCTTTCTGACGGGCTGTGTGAATGTAATAAAGGCTACACTGGTGTTGACTGCTCCACTGCGGTTTGTGATGAGCAATGCAGCCTTCATGGAGGTGTTTGTGATGATGGAGTCTGTGAATTCCGCTGCTCTGACTATGCAGGCTACTCATGCCAGAACAGCACCATGCTCCAGTCCAGTCTTAAAGTTTGTAAAGATGTGCTGGAAAACGTCAACTCTGGTGCCGGACAGCACTGTGCACCCAGTGAACCAAGCATATTGCAGCAGCTTGAGGATGTAGTGGTCATGCCCAACTACCACCGTTTGTTTCCTGGTGGCGCCCGGAAACTTTTTAGCATCTTCAGAACCAGCTACTGTGATATGACCGCGAAGCAGCTGGCTTGCTGGATATCAATTCAAAAGTGTGACAAGGACGGGGACAACAGGCTCCGCGTCTGCTATTCAGCTTGCCAATCGTACAATTCGGCATGCGGGGCTTCGCTCGATTGCTCGGATCGAACCCTATTCAGTAGTAAAGACGAAGCACAAGGGCAATGCACAGGCTCTAGTGAGATGAGAACGTCATGGATTAGCAGCATATATAGTTGGTTTTCAAACAACAGTTCCTCAAAAGGGATGTCTGTAAAAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000070 GO:0000226 GO:0000278 GO:0000280 GO:0000819 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006323 GO:0006325 GO:0006338 GO:0006508 GO:0006807 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0007052 GO:0007059 GO:0007076 GO:0007088 GO:0007098 GO:0007100 GO:0007275 GO:0007276 GO:0007346 GO:0007399 GO:0007417 GO:0007420 GO:0007444 GO:0007548 GO:0008150 GO:0008152 GO:0008233 GO:0008354 GO:0008406 GO:0009888 GO:0009896 GO:0009987 GO:0010564 GO:0010638 GO:0010965 GO:0016043 GO:0016477 GO:0016787 GO:0019538 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0030071 GO:0030261 GO:0031023 GO:0031331 GO:0032270 GO:0032436 GO:0032501 GO:0032502 GO:0032504 GO:0033043 GO:0033044 GO:0033045 GO:0033047 GO:0035295 GO:0040011 GO:0043170 GO:0044238 GO:0044424 GO:0044464 GO:0044703 GO:0045137 GO:0045732 GO:0045787 GO:0045840 GO:0045842 GO:0045862 GO:0045931 GO:0048285 GO:0048513 GO:0048518 GO:0048522 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0048870 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051247 GO:0051276 GO:0051298 GO:0051299 GO:0051674 GO:0051704 GO:0051726 GO:0051781 GO:0051783 GO:0051785 GO:0051983 GO:0051984 GO:0060322 GO:0060429 GO:0061458 GO:0062033 GO:0065007 GO:0071103 GO:0071704 GO:0071840 GO:0090068 GO:0098813 GO:0140014 GO:0140096 GO:1901564 GO:1901800 GO:1901970 GO:1901987 GO:1901989 GO:1901990 GO:1901992 GO:1902099 GO:1902101 GO:1902850 GO:1903047 GO:1903052 GO:1903364 GO:1905818 GO:1905820 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko05140 ko05143 map05140 map05143
Pfam Domains
Protein Families
EGF_2 Peptidase_M8

Protein Analysis

813

Amino Acids

89.27

Weight (kDa)

8.08

Isoelectric Point (pI)

48.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M8 PF01457 169 - 262 5e-09 Leishmanolysin
Peptidase_M8 PF01457 292 - 553 3e-50 Leishmanolysin
EGF_TEN PF25024 565 - 768 3.7e-11 Teneurin EGF domain
EGF_Teneurin PF23106 593 - 622 3e-06 Teneurin-like EGF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010930)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42620 AT5G42620
fragaria_vesca FvH4_2g40290 FvH4_2g40290 FvH4_2g40290
malus_domestica MD08G1036300.v1.1
prunus_persica Prupe.1G383600_v2.0.a1
pyrus_communis pycom08g02900 pycom15g02830
rosa_chinensis RchiOBHm_Chr6g0300451
rosa_laevigata RLG00000011336
rosa_multiflora Rmu_sc0001670.1_g000004 Rmu_sc0014364.1_g000001
rosa_roxburghii Rroxscaffold_7G00167770
rosa_rugosa Rorug06G0296200
rosa_samantha Rh6AG408800 Rh6BG416000 Rh6CG423500 Rh6DG409200
rosa_wichuraiana Rw6G035770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1784
Acc36I ACCTGC 1 cut(s) 1784
Acc65I GGTACC 1 cut(s) 1098
AccB1I GGYRCC 3 cut(s) 1098, 2027, 2123
AccB7I CCANNNNNTGG 1 cut(s) 491
AccII CGCG 2 cut(s) 2174, 2238
AclWI GGATC 4 cut(s) 473, 1044, 1384, 2307
AcoI YGGCCR 1 cut(s) 652
AcsI RAATTY 4 cut(s) 300, 940, 1157, 1925
AcuI CTGAAG 4 cut(s) 711, 1583, 1602, 2131
AcvI CACGTG 1 cut(s) 896
AcyI GRCGYC 1 cut(s) 2124
AdeI CACNNNGTG 2 cut(s) 683, 2051
AfeI AGCGCT 1 cut(s) 1399
AfiI CCNNNNNNNGG 9 cut(s) 491, 559, 617, 731, 747, 748, 1586, 1676, 2418
AflIII ACRYGT 1 cut(s) 895
AgsI TTSAA 8 cut(s) 589, 608, 912, 1062, 1705, 1721, 2204, 2400
AjiI CACGTC 1 cut(s) 1799
AjnI CCWGG 8 cut(s) 208, 448, 654, 699, 1101, 1308, 1606, 2116
Alw21I GWGCWC 1 cut(s) 2047
Alw26I GTCTC 2 cut(s) 1272, 1704
Alw44I GTGCAC 1 cut(s) 2043
AlwI GGATC 4 cut(s) 473, 1044, 1384, 2307
AlwNI CAGNNNCTG 1 cut(s) 2160
Aor51HI AGCGCT 1 cut(s) 1399
AoxI GGCC 4 cut(s) 338, 652, 791, 1138
ApaLI GTGCAC 1 cut(s) 2043
ApeKI GCWGC 7 cut(s) 1806, 1887, 1932, 2068, 2071, 2179, 2379
ApoI RAATTY 4 cut(s) 300, 940, 1157, 1925
ArsI GACNNNNNNTTYG 1 cut(s) 2408
Asp718I GGTACC 1 cut(s) 1098
AspLEI GCGC 3 cut(s) 201, 1400, 2126
AspS9I GGNCC 3 cut(s) 791, 1138, 1592
AsuC2I CCSGG 2 cut(s) 1634, 2128
AsuHPI GGTGA 7 cut(s) 19, 266, 488, 506, 724, 886, 1436
AvaII GGWCC 1 cut(s) 1592
BaeGI GKGCMC 2 cut(s) 1297, 2047
BanI GGYRCC 3 cut(s) 1098, 2027, 2123
BanII GRGCYC 1 cut(s) 598
BauI CACGAG 2 cut(s) 471, 879
BbrPI CACGTG 1 cut(s) 896
Bbv12I GWGCWC 1 cut(s) 2047
BbvI GCAGC 7 cut(s) 1793, 1899, 1919, 2080, 2083, 2191, 2391
BccI CCATC 6 cut(s) 653, 959, 1089, 1367, 1553, 1907
BceAI ACGGC 2 cut(s) 32, 778
BcgI CGANNNNNNTGC 4 cut(s) 194, 228, 1339, 1373
BciT130I CCWGG 8 cut(s) 210, 450, 656, 701, 1103, 1310, 1608, 2118
BciVI GTATCC 1 cut(s) 1036
BcnI CCSGG 2 cut(s) 1634, 2128
BcoDI GTCTC 2 cut(s) 1272, 1704
BfaI CTAG 8 cut(s) 93, 566, 599, 959, 1446, 1664, 1742, 2352
BfmI CTRYAG 1 cut(s) 1651
BfoI RGCGCY 2 cut(s) 1401, 2127
BfuAI ACCTGC 1 cut(s) 1784
BfuI GTATCC 1 cut(s) 1036
BmcAI AGTACT 1 cut(s) 311
Bme18I GGWCC 1 cut(s) 1592
BmgBI CACGTC 1 cut(s) 1799
BmgT120I GGNCC 3 cut(s) 791, 1138, 1592
BmiI GGNNCC 8 cut(s) 429, 792, 1100, 1139, 1594, 2029, 2125, 2234
BmrI ACTGGG 4 cut(s) 741, 1006, 1282, 2043
BmsI GCATC 4 cut(s) 3, 110, 355, 2151
BmuI ACTGGG 4 cut(s) 741, 1006, 1282, 2043
BpmI CTGGAG 1 cut(s) 1961
BpuEI CTTGAG 1 cut(s) 2096
BpuMI CCSGG 2 cut(s) 1634, 2128
Bsa29I ATCGAT 1 cut(s) 781
BsaAI YACGTR 2 cut(s) 896, 1472
BsaHI GRCGYC 1 cut(s) 2124
BsaI GGTCTC 1 cut(s) 1272
BsaJI CCNNGG 6 cut(s) 1053, 1147, 1308, 1464, 1501, 1606
BsaXI ACNNNNNCTCC 4 cut(s) 537, 567, 1701, 1731
Bsc4I CCNNNNNNNGG 9 cut(s) 491, 559, 617, 731, 747, 748, 1586, 1676, 2418
Bse1I ACTGG 9 cut(s) 587, 736, 1001, 1288, 1341, 1852, 1978, 1983, 2049
Bse3DI GCAATG 2 cut(s) 1889, 2345
BseBI CCWGG 8 cut(s) 210, 450, 656, 701, 1103, 1310, 1608, 2118
BseCI ATCGAT 1 cut(s) 781
BseDI CCNNGG 6 cut(s) 1053, 1147, 1308, 1464, 1501, 1606
BseLI CCNNNNNNNGG 9 cut(s) 491, 559, 617, 731, 747, 748, 1586, 1676, 2418
BseMI GCAATG 2 cut(s) 1889, 2345
BseMII CTCAG 1 cut(s) 927
BseNI ACTGG 9 cut(s) 587, 736, 1001, 1288, 1341, 1852, 1978, 1983, 2049
BseSI GKGCMC 2 cut(s) 1297, 2047
BseXI GCAGC 7 cut(s) 1793, 1899, 1919, 2080, 2083, 2191, 2391
BseYI CCCAGC 2 cut(s) 530, 804
BsgI GTGCAG 1 cut(s) 496
Bsh1236I CGCG 2 cut(s) 2174, 2238
BshFI GGCC 4 cut(s) 340, 654, 793, 1140
BshNI GGYRCC 3 cut(s) 1098, 2027, 2123
BshVI ATCGAT 1 cut(s) 781
BsiHKAI GWGCWC 1 cut(s) 2047
BsiSI CCGG 3 cut(s) 1634, 2031, 2128
BslFI GGGAC 2 cut(s) 1315, 2237
BslI CCNNNNNNNGG 9 cut(s) 491, 559, 617, 731, 747, 748, 1586, 1676, 2418
BsmAI GTCTC 2 cut(s) 1272, 1704
BsmFI GGGAC 2 cut(s) 1315, 2237
BsmI GAATGC 3 cut(s) 174, 907, 929
BsnI GGCC 4 cut(s) 340, 654, 793, 1140
Bso31I GGTCTC 1 cut(s) 1272
Bsp1286I GDGCHC 3 cut(s) 598, 1297, 2047
Bsp1407I TGTACA 4 cut(s) 222, 316, 667, 1226
Bsp143I GATC 6 cut(s) 478, 778, 782, 1049, 1376, 2299
BspANI GGCC 4 cut(s) 340, 654, 793, 1140
BspCNI CTCAG 1 cut(s) 928
BspDI ATCGAT 1 cut(s) 781
BspFNI CGCG 2 cut(s) 2174, 2238
BspHI TCATGA 1 cut(s) 178
BspLI GGNNCC 8 cut(s) 429, 792, 1100, 1139, 1594, 2029, 2125, 2234
BspMI ACCTGC 1 cut(s) 1784
BspPI GGATC 4 cut(s) 473, 1044, 1384, 2307
BspT107I GGYRCC 3 cut(s) 1098, 2027, 2123
BspTNI GGTCTC 1 cut(s) 1272
BsrDI GCAATG 2 cut(s) 1889, 2345
BsrGI TGTACA 4 cut(s) 222, 316, 667, 1226
BsrI ACTGG 9 cut(s) 587, 736, 1001, 1288, 1341, 1852, 1978, 1983, 2049
BssECI CCNNGG 6 cut(s) 1053, 1147, 1308, 1464, 1501, 1606
BssMI GATC 6 cut(s) 478, 778, 782, 1049, 1376, 2299
BssNI GRCGYC 1 cut(s) 2124
BssSI CACGAG 2 cut(s) 471, 879
BssT1I CCWWGG 3 cut(s) 1147, 1464, 1501
Bst2BI CACGAG 2 cut(s) 471, 879
Bst2UI CCWGG 8 cut(s) 210, 450, 656, 701, 1103, 1310, 1608, 2118
Bst6I CTCTTC 1 cut(s) 450
BstACI GRCGYC 1 cut(s) 2124
BstAUI TGTACA 4 cut(s) 222, 316, 667, 1226
BstBAI YACGTR 2 cut(s) 896, 1472
BstDEI CTNAG 1 cut(s) 936
BstDSI CCRYGG 1 cut(s) 1053
BstENI CCTNNNNNAGG 1 cut(s) 557
BstFNI CGCG 2 cut(s) 2174, 2238
BstH2I RGCGCY 2 cut(s) 1401, 2127
BstHHI GCGC 3 cut(s) 201, 1400, 2126
BstKTI GATC 6 cut(s) 481, 781, 785, 1052, 1379, 2302
BstMAI GTCTC 2 cut(s) 1272, 1704
BstMBI GATC 6 cut(s) 478, 778, 782, 1049, 1376, 2299
BstMWI GCNNNNNNNGC 5 cut(s) 98, 427, 1956, 1965, 2279
BstNI CCWGG 8 cut(s) 210, 450, 656, 701, 1103, 1310, 1608, 2118
BstNSI RCATGY 2 cut(s) 651, 2277
BstSFI CTRYAG 1 cut(s) 1651
BstSLI GKGCMC 2 cut(s) 1297, 2047
BstSNI TACGTA 1 cut(s) 1472
BstUI CGCG 2 cut(s) 2174, 2238
BstV1I GCAGC 7 cut(s) 1793, 1899, 1919, 2080, 2083, 2191, 2391
BstX2I RGATCY 1 cut(s) 1049
BstXI CCANNNNNNTGG 1 cut(s) 1508
BstYI RGATCY 1 cut(s) 1049
Bsu15I ATCGAT 1 cut(s) 781
BsuI GTATCC 1 cut(s) 1036
BsuRI GGCC 4 cut(s) 340, 654, 793, 1140
BsuTUI ATCGAT 1 cut(s) 781
BtgI CCRYGG 1 cut(s) 1053
BtgZI GCGATG 1 cut(s) 423
BtrI CACGTC 1 cut(s) 1799
BtsI GCAGTG 3 cut(s) 759, 1198, 1863
BveI ACCTGC 1 cut(s) 1784
CaiI CAGNNNCTG 1 cut(s) 2160
CciI TCATGA 1 cut(s) 178
CfoI GCGC 3 cut(s) 201, 1400, 2126
Cfr13I GGNCC 3 cut(s) 791, 1138, 1592
ClaI ATCGAT 1 cut(s) 781
CseI GACGC 1 cut(s) 2227
CsiI ACCWGGT 2 cut(s) 208, 699
CspCI CAANNNNNGTGG 2 cut(s) 1035, 1070
DdeI CTNAG 1 cut(s) 936
DinI GGCGCC 1 cut(s) 2125
DpnI GATC 6 cut(s) 480, 780, 784, 1051, 1378, 2301
DpnII GATC 6 cut(s) 478, 778, 782, 1049, 1376, 2299
DraI TTTAAA 1 cut(s) 357
DraIII CACNNNGTG 2 cut(s) 683, 2051
EaeI YGGCCR 1 cut(s) 652
Eam1104I CTCTTC 1 cut(s) 450
EarI CTCTTC 1 cut(s) 450
EciI GGCGGA 1 cut(s) 1136
Eco105I TACGTA 1 cut(s) 1472
Eco130I CCWWGG 3 cut(s) 1147, 1464, 1501
Eco147I AGGCCT 1 cut(s) 340
Eco24I GRGCYC 1 cut(s) 598
Eco31I GGTCTC 1 cut(s) 1272
Eco32I GATATC 2 cut(s) 917, 2196
Eco47I GGWCC 1 cut(s) 1592
Eco47III AGCGCT 1 cut(s) 1399
Eco57I CTGAAG 4 cut(s) 711, 1583, 1602, 2131
Eco72I CACGTG 1 cut(s) 896
EcoNI CCTNNNNNAGG 1 cut(s) 557
EcoRI GAATTC 1 cut(s) 1925
EcoRII CCWGG 8 cut(s) 208, 448, 654, 699, 1101, 1308, 1606, 2116
EcoRV GATATC 2 cut(s) 917, 2196
EcoT14I CCWWGG 3 cut(s) 1147, 1464, 1501
EcoT22I ATGCAT 2 cut(s) 103, 909
EcoT38I GRGCYC 1 cut(s) 598
EgeI GGCGCC 1 cut(s) 2125
EheI GGCGCC 1 cut(s) 2125
ErhI CCWWGG 3 cut(s) 1147, 1464, 1501
FaqI GGGAC 2 cut(s) 1315, 2237
FauI CCCGC 2 cut(s) 243, 2270
FriOI GRGCYC 1 cut(s) 598
FspBI CTAG 8 cut(s) 93, 566, 599, 959, 1446, 1664, 1742, 2352
GlaI GCGC 3 cut(s) 200, 1399, 2125
GsaI CCCAGC 2 cut(s) 534, 808
GsuI CTGGAG 1 cut(s) 1961
HaeII RGCGCY 2 cut(s) 1401, 2127
HaeIII GGCC 4 cut(s) 340, 654, 793, 1140
HapII CCGG 3 cut(s) 1634, 2031, 2128
HgaI GACGC 1 cut(s) 2227
HhaI GCGC 3 cut(s) 201, 1400, 2126
Hin1I GRCGYC 1 cut(s) 2124
Hin6I GCGC 3 cut(s) 199, 1398, 2124
HinP1I GCGC 3 cut(s) 199, 1398, 2124
HincII GTYRAC 2 cut(s) 1855, 2020
HindII GTYRAC 2 cut(s) 1855, 2020
HindIII AAGCTT 2 cut(s) 419, 860
HinfI GANTC 3 cut(s) 42, 877, 1917
HpaII CCGG 3 cut(s) 1634, 2031, 2128
HphI GGTGA 7 cut(s) 19, 266, 488, 506, 724, 886, 1436
Hpy166II GTNNAC 9 cut(s) 223, 225, 668, 1592, 1769, 1855, 2020, 2045, 2054
Hpy188I TCNGA 8 cut(s) 151, 937, 1372, 1759, 1816, 1939, 2150, 2299
Hpy188III TCNNGA 8 cut(s) 179, 383, 589, 912, 1130, 1183, 1221, 1580
Hpy8I GTNNAC 9 cut(s) 223, 225, 668, 1592, 1769, 1855, 2020, 2045, 2054
Hpy99I CGWCG 3 cut(s) 53, 56, 59
HpyCH4IV ACGT 6 cut(s) 766, 895, 1471, 1798, 2016, 2366
HpyF10VI GCNNNNNNNGC 5 cut(s) 98, 427, 1956, 1965, 2279
HpyF3I CTNAG 1 cut(s) 936
HpySE526I ACGT 6 cut(s) 766, 895, 1471, 1798, 2016, 2366
Hsp92I GRCGYC 1 cut(s) 2124
HspAI GCGC 3 cut(s) 199, 1398, 2124
KasI GGCGCC 1 cut(s) 2123
KpnI GGTACC 1 cut(s) 1102
Kzo9I GATC 6 cut(s) 478, 778, 782, 1049, 1376, 2299
LmnI GCTCC 4 cut(s) 427, 1865, 1980, 2238
Lsp1109I GCAGC 7 cut(s) 1793, 1899, 1919, 2080, 2083, 2191, 2391
LweI GCATC 4 cut(s) 3, 110, 355, 2151
MabI ACCWGGT 2 cut(s) 208, 699
MaeI CTAG 8 cut(s) 93, 566, 599, 959, 1446, 1664, 1742, 2352
MaeII ACGT 6 cut(s) 766, 895, 1471, 1798, 2016, 2366
MalI GATC 6 cut(s) 480, 780, 784, 1051, 1378, 2301
MboI GATC 6 cut(s) 478, 778, 782, 1049, 1376, 2299
MboII GAAGA 7 cut(s) 437, 698, 881, 974, 1097, 1737, 2137
MfeI CAATTG 2 cut(s) 799, 1260
MflI RGATCY 1 cut(s) 1049
MhlI GDGCHC 3 cut(s) 598, 1297, 2047
Mly113I GGCGCC 1 cut(s) 2124
MlyI GAGTC 2 cut(s) 871, 1926
Mph1103I ATGCAT 2 cut(s) 103, 909
MseI TTAA 5 cut(s) 333, 356, 1533, 1614, 1989
MslI CAYNNNNRTG 1 cut(s) 1899
MspA1I CMGCKG 2 cut(s) 1932, 2182
MspI CCGG 3 cut(s) 1634, 2031, 2128
MunI CAATTG 2 cut(s) 799, 1260
Mva1269I GAATGC 3 cut(s) 174, 907, 929
MvaI CCWGG 8 cut(s) 210, 450, 656, 701, 1103, 1310, 1608, 2118
MvnI CGCG 2 cut(s) 2174, 2238
MwoI GCNNNNNNNGC 5 cut(s) 98, 427, 1956, 1965, 2279
NarI GGCGCC 1 cut(s) 2124
NciI CCSGG 2 cut(s) 1634, 2128
NdeII GATC 6 cut(s) 478, 778, 782, 1049, 1376, 2299
NlaIV GGNNCC 8 cut(s) 429, 792, 1100, 1139, 1594, 2029, 2125, 2234
NmuCI GTSAC 6 cut(s) 374, 712, 874, 990, 1730, 2210
NsiI ATGCAT 2 cut(s) 103, 909
NspI RCATGY 2 cut(s) 651, 2277
PaeI GCATGC 2 cut(s) 651, 2277
PagI TCATGA 1 cut(s) 178
PaqCI CACCTGC 1 cut(s) 1784
PceI AGGCCT 1 cut(s) 340
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PctI GAATGC 3 cut(s) 174, 907, 929
PfeI GAWTC 1 cut(s) 42
PflFI GACNNNGTC 2 cut(s) 1033, 1683
PflMI CCANNNNNTGG 1 cut(s) 491
PleI GAGTC 2 cut(s) 871, 1925
PluTI GGCGCC 1 cut(s) 2127
PmaCI CACGTG 1 cut(s) 896
PmlI CACGTG 1 cut(s) 896
PpsI GAGTC 2 cut(s) 871, 1925
Ppu21I YACGTR 2 cut(s) 896, 1472
Psp6I CCWGG 8 cut(s) 208, 448, 654, 699, 1101, 1308, 1606, 2116
PspCI CACGTG 1 cut(s) 896
PspFI CCCAGC 2 cut(s) 530, 804
PspGI CCWGG 8 cut(s) 208, 448, 654, 699, 1101, 1308, 1606, 2116
PspN4I GGNNCC 8 cut(s) 429, 792, 1100, 1139, 1594, 2029, 2125, 2234
PspPI GGNCC 3 cut(s) 791, 1138, 1592
PstNI CAGNNNCTG 1 cut(s) 2160
PsuI RGATCY 1 cut(s) 1049
PsyI GACNNNGTC 2 cut(s) 1033, 1683
PvuII CAGCTG 1 cut(s) 2182
RseI CAYNNNNRTG 1 cut(s) 1899
SaqAI TTAA 5 cut(s) 333, 356, 1533, 1614, 1989
Sau3AI GATC 6 cut(s) 478, 778, 782, 1049, 1376, 2299
Sau96I GGNCC 3 cut(s) 791, 1138, 1592
ScaI AGTACT 1 cut(s) 311
SchI GAGTC 2 cut(s) 871, 1926
SduI GDGCHC 3 cut(s) 598, 1297, 2047
SexAI ACCWGGT 2 cut(s) 208, 699
SfaNI GCATC 4 cut(s) 3, 110, 355, 2151
SfcI CTRYAG 1 cut(s) 1651
SfoI GGCGCC 1 cut(s) 2125
SinI GGWCC 1 cut(s) 1592
SmiI ATTTAAAT 1 cut(s) 357
SmiMI CAYNNNNRTG 1 cut(s) 1899
SmlI CTYRAG 1 cut(s) 2075
SmoI CTYRAG 1 cut(s) 2075
SnaBI TACGTA 1 cut(s) 1472
SphI GCATGC 2 cut(s) 651, 2277
SseBI AGGCCT 1 cut(s) 340
SspDI GGCGCC 1 cut(s) 2123
SspMI CTAG 8 cut(s) 93, 566, 599, 959, 1446, 1664, 1742, 2352
StuI AGGCCT 1 cut(s) 340
StyI CCWWGG 3 cut(s) 1147, 1464, 1501
SwaI ATTTAAAT 1 cut(s) 357
TaiI ACGT 6 cut(s) 769, 898, 1474, 1801, 2019, 2369
TaqI TCGA 4 cut(s) 781, 1402, 2289, 2302
TatI WGTACW 6 cut(s) 222, 309, 316, 667, 1226, 1382
TauI GCSGC 3 cut(s) 19, 654, 1143
TfiI GAWTC 1 cut(s) 42
Tru1I TTAA 5 cut(s) 333, 356, 1533, 1614, 1989
Tru9I TTAA 5 cut(s) 333, 356, 1533, 1614, 1989
TseFI GTSAC 6 cut(s) 374, 712, 874, 990, 1730, 2210
TseI GCWGC 7 cut(s) 1806, 1887, 1932, 2068, 2071, 2179, 2379
Tsp45I GTSAC 6 cut(s) 374, 712, 874, 990, 1730, 2210
TspDTI ATGAA 8 cut(s) 167, 870, 1031, 1035, 1449, 1659, 1739, 1883
TspGWI ACGGA 1 cut(s) 1042
Tth111I GACNNNGTC 2 cut(s) 1033, 1683
Van91I CCANNNNNTGG 1 cut(s) 491
VneI GTGCAC 1 cut(s) 2043
VpaK11BI GGWCC 1 cut(s) 1592
XagI CCTNNNNNAGG 1 cut(s) 557
XapI RAATTY 4 cut(s) 300, 940, 1157, 1925
XceI RCATGY 2 cut(s) 651, 2277
XspI CTAG 8 cut(s) 93, 566, 599, 959, 1446, 1664, 1742, 2352
ZrmI AGTACT 1 cut(s) 311
Zsp2I ATGCAT 2 cut(s) 103, 909
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.