pycom15g08400

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0017629
Physical Location & Seq
Reverse (-)
5262753 .. 5265097
2345 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2121 bp
ATGCGCCACCTCTACCAAATCCAAGCCCAACTCCTTACCAACCCAATCCCCTCCATCGACCCAAACATCATCGCCGTCAAGCTCATCGGCGTCTCTGCCGCCCACGCCAACACCCGCCACGCTACACTCGTATTTAACCACCCCCTCAAAACGCCGAATATTTTCTGTTACAACGCCCTTCTCAAAGCATTCGCCCAAAACAATGACTGGCCAAACACGATATATTTTTTCAATCGGCAATTGGGTTCGCCAGATGCTCCGGACCCGGACGAGTACACCTTCACTTCGGTGCTCAAGGCCTGCGCCGGGATTGCACGAGTGGCGGAGGGCGAGAAAGTTCACTGCTTTGTTTTGAAATACGGGTGTGAATCCAATCTGTTTGTTAGGAATTCGCTAACTGATATGTATTTCAAGGTGGGTAATTTTGGGATTGCCCAGAAGCTGTTTGATGATATGTCTGTGAGAGATGTTGTTTCCTGGAACACTTTGGTTTCCGGGTATTGTTCGAGTGGGCAGGTTGAGAAGGCTAGGTGGGTGTTTGATGGGATGGAAGAGAAGACTTTGTTTTCTTGGTCGACTATGATCAGTGCGTATGCGAAGGCAGGTGAGTTGGAGGTGGCTCAGCGGCTTTTTGATGAGATGCCGAAGAGGAATGTGGTTTCTTGGAATGCAATAATTGCGGGGTATGCACAGAATGAGATGTATGCTGAGGCTATTCAGATGTTTAGGCAAATGCAGCGATGTGGGGTGACGCCAAATGATGTTACGCTGATCAGTGTGCTGTCTGCTTGCGCTCACCTTGGTGCTCTTGACTTGGGGAAGTGGATTGATCGGTTTATAAGGCGGAATGGGATGGAATTAGGTCTGTTTTTGGGGAATGCATTGGCGGATATGTATGCGAAGTGCGGCTGCATAATTGAGGCTAAACGAGTTTTTAACAAGATGCACGAGAGAGATGTGATATCTTGGAGCATTATCATCACTGGATTGGCCATGAATGGCCATGCTGATGAAGCTTTCGGGTGTTTCGACAAAATGATTGAGTATGGGGAGAAGCCAAATGACATCACTTTTATGGGATTGTTGACAGCATGTACACATGCTGGCTTGGTCGATAAGGGGCTTGAGTATTTTGATTTGATGGCCAAAGCATACGGGATCCTTCCCAAGGTTGAGCATTATGGATGTGTGGTTGATCTTCTCAGTCGTGCCGGCCGCCTTATTGAAGCAGAGGATATGATCAACACAATGCCGATGCGGCCTAATGTTATAGTCTGGGGAGCATTGCTAGGTGGTTGCCGGATTTATAAAGATAGTGAGAGAGGGGAACGTGTAGTTCAGCGTATTCTTGAACTAGATTCCGATCATTCTGGAAGCTATGTTTATCTTGCTAATGTGTATACGTCAATGGGTAGGCTAGACGATGCAGCAAGATGTAGGCTGCAAATGCGAGATAAAGGCGTATCGAAAACACCTGGATGCAGCTGGATAGAGGTGGACAACATAGTTCATGAGTTCTTCATGGGAGACCTGTCGCATCCTCAGATGGACAAGATATATTCGATGATTAGAGAACTAAGGAGGAAAATGAAGCTAGCTGGGTACAAACCGAAGACGGATTTAGTCCTACATAATATCGATGAAGAAGAGAAAGAGGATGCATTATTGGTTCACAGCGAGAGGCAAGCGATTGCATTCGGGCTGATTAGTACCAGTCCTGGAACCACAATTCGAATCGTGAAGAATTTGCGAGTTTGTCACGATTGCCACGATGCAACGAAATTAATCTCTAAAATTGTTGAGCGAGAGATTGTTGTGCGCGATCGCAGTCGGTTCCACCATTTCAAAGATGGGAAATTTCGAAATTTCGGTTTTGGATTCAAAACCGAACGGCTTCCAAAACCGTTCAGATTTTGGTGGAAATGCACAAGCTTACTCAAAAGTGGTCACTATCTTCAGTCAAACAATGAAAAGGCAAAAATGTTTCCTGTTCCCGTGTCTTTCACCCAACTCATGTTCCGAGAAACCAAAAAACTTGGTGCAACTACCAGTTTCACACAAGGCATTTCCATTGATAACGACACCACTTACATATGGTTTCCGGTGAAAGGAAGAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

707

Amino Acids

80.13

Weight (kDa)

8.55

Isoelectric Point (pI)

34.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 155 - 186 9.6e-07 PPR repeat family
PPR_1 PF12854 155 - 184 1.5e-07 PPR repeat
PPR PF01535 158 - 186 3.9e-08 PPR repeat
PPR_2 PF13041 188 - 219 1.5e-06 PPR repeat family
PPR_1 PF12854 188 - 214 5e-06 PPR repeat
PPR PF01535 189 - 219 4.3e-08 PPR repeat
PPR_2 PF13041 217 - 266 6.4e-13 PPR repeat family
PPR PF01535 220 - 250 1.3e-07 PPR repeat
PPR_2 PF13041 318 - 366 1e-09 PPR repeat family
E_motif PF20431 436 - 498 1.7e-18 E motif
DYW_deaminase PF14432 534 - 619 6.7e-37 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017629)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g24230
malus_domestica MD15G1089600.v1.1
prunus_persica Prupe.1G444200_v2.0.a1
pyrus_communis pycom15g08400
rosa_chinensis RchiOBHm_Chr5g0043351
rosa_laevigata RLG00000034182
rosa_multiflora Rmu_sc0000060.1_g000011 Rmu_sc0000060.1_g000039
rosa_roxburghii Rroxscaffold_1G00037500
rosa_rugosa Rorug05G0205400
rosa_samantha Rh5CG327400
rosa_wichuraiana Rw5G027000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 839, 1308
AarI CACCTGC 1 cut(s) 593
Acc36I ACCTGC 2 cut(s) 505, 593
AccI GTMKAC 2 cut(s) 575, 1400
AccII CGCG 1 cut(s) 1821
AccIII TCCGGA 1 cut(s) 259
AclWI GGATC 2 cut(s) 1153, 1166
AcoI YGGCCR 5 cut(s) 209, 990, 1000, 1143, 1213
AcsI RAATTY 4 cut(s) 388, 1744, 1856, 1864
AcuI CTGAAG 1 cut(s) 1940
AcyI GRCGYC 2 cut(s) 90, 752
AfaI GTAC 4 cut(s) 275, 1096, 1604, 1711
AfiI CCNNNNNNNGG 3 cut(s) 306, 1168, 2108
AflIII ACRYGT 1 cut(s) 1330
AgsI TTSAA 7 cut(s) 232, 355, 412, 1226, 1352, 1846, 1882
AjnI CCWGG 3 cut(s) 476, 1474, 1717
AleI CACNNNNGTG 2 cut(s) 287, 801
AluBI AGCT 8 cut(s) 82, 442, 1016, 1377, 1485, 1594, 1598, 1932
AluI AGCT 8 cut(s) 82, 442, 1016, 1377, 1485, 1594, 1598, 1932
Alw21I GWGCWC 2 cut(s) 294, 808
Alw26I GTCTC 2 cut(s) 97, 1521
AlwI GGATC 2 cut(s) 1153, 1166
AlwNI CAGNNNCTG 1 cut(s) 442
Aor13HI TCCGGA 1 cut(s) 259
AoxI GGCC 7 cut(s) 209, 297, 990, 1000, 1143, 1213, 1259
ApeKI GCWGC 5 cut(s) 736, 909, 1427, 1441, 1482
ApoI RAATTY 4 cut(s) 388, 1744, 1856, 1864
AseI ATTAAT 1 cut(s) 1784
AsiSI GCGATCGC 1 cut(s) 1825
Asp700I GAANNNNTTC 2 cut(s) 161, 1893
AspLEI GCGC 4 cut(s) 6, 305, 794, 1821
AspS9I GGNCC 1 cut(s) 262
AsuC2I CCSGG 3 cut(s) 266, 307, 496
AsuHPI GGTGA 5 cut(s) 617, 760, 788, 1996, 2116
AsuII TTCGAA 2 cut(s) 1732, 1861
AsuNHI GCTAGC 1 cut(s) 1594
AvaII GGWCC 1 cut(s) 262
BalI TGGCCA 4 cut(s) 211, 992, 1002, 1145
BamHI GGATCC 1 cut(s) 1158
BauI CACGAG 2 cut(s) 315, 947
BbsI GAAGAC 2 cut(s) 563, 1619
Bbv12I GWGCWC 2 cut(s) 294, 808
BbvCI CCTCAGC 1 cut(s) 708
BbvI GCAGC 5 cut(s) 748, 896, 1428, 1439, 1494
BccI CCATC 7 cut(s) 62, 536, 541, 847, 1135, 1540, 1844
BceAI ACGGC 2 cut(s) 59, 1907
BciT130I CCWGG 3 cut(s) 478, 1476, 1719
BclI TGATCA 3 cut(s) 582, 771, 1239
BcnI CCSGG 3 cut(s) 266, 307, 496
BcoDI GTCTC 2 cut(s) 97, 1521
BfaI CTAG 5 cut(s) 528, 1289, 1355, 1418, 1595
BfuAI ACCTGC 2 cut(s) 505, 593
BglI GCCNNNNNGGC 1 cut(s) 1258
BlpI GCTNAGC 1 cut(s) 621
Bme1390I CCNGG 6 cut(s) 266, 307, 478, 496, 1476, 1719
Bme18I GGWCC 1 cut(s) 262
BmgT120I GGNCC 1 cut(s) 262
BmiI GGNNCC 4 cut(s) 264, 1160, 1723, 1835
BmrFI CCNGG 6 cut(s) 266, 307, 478, 496, 1476, 1719
BmsI GCATC 9 cut(s) 244, 630, 933, 1245, 1414, 1469, 1546, 1648, 1762
BmtI GCTAGC 1 cut(s) 1598
BpiI GAAGAC 2 cut(s) 563, 1619
Bpu10I CCTNAGC 1 cut(s) 708
Bpu1102I GCTNAGC 1 cut(s) 621
Bpu14I TTCGAA 2 cut(s) 1732, 1861
BpuEI CTTGAG 2 cut(s) 278, 1145
BpuMI CCSGG 3 cut(s) 266, 307, 496
Bsa29I ATCGAT 1 cut(s) 1638
BsaBI GATNNNNATC 1 cut(s) 1362
BsaHI GRCGYC 2 cut(s) 90, 752
BsaI GGTCTC 1 cut(s) 1521
BsaJI CCNNGG 2 cut(s) 799, 1167
BsaWI WCCGGW 2 cut(s) 259, 2101
Bsc4I CCNNNNNNNGG 3 cut(s) 306, 1168, 2108
Bse118I RCCGGY 1 cut(s) 1211
Bse1I ACTGG 4 cut(s) 212, 988, 1713, 2049
Bse3DI GCAATG 1 cut(s) 1283
Bse8I GATNNNNATC 1 cut(s) 1362
BseAI TCCGGA 1 cut(s) 259
BseBI CCWGG 3 cut(s) 478, 1476, 1719
BseCI ATCGAT 1 cut(s) 1638
BseDI CCNNGG 2 cut(s) 799, 1167
BseGI GGATG 6 cut(s) 552, 858, 1190, 1484, 1537, 1663
BseJI GATNNNNATC 1 cut(s) 1362
BseLI CCNNNNNNNGG 3 cut(s) 306, 1168, 2108
BseMI GCAATG 1 cut(s) 1283
BseMII CTCAG 4 cut(s) 635, 699, 1216, 1556
BseNI ACTGG 4 cut(s) 212, 988, 1713, 2049
BseX3I CGGCCG 1 cut(s) 1213
BseXI GCAGC 5 cut(s) 748, 896, 1428, 1439, 1494
BseYI CCCAGC 1 cut(s) 1598
Bsh1236I CGCG 1 cut(s) 1821
Bsh1285I CGRYCG 2 cut(s) 1216, 1825
BshFI GGCC 7 cut(s) 211, 299, 992, 1002, 1145, 1215, 1261
BshVI ATCGAT 1 cut(s) 1638
BsiEI CGRYCG 2 cut(s) 1216, 1825
BsiHKAI GWGCWC 2 cut(s) 294, 808
BsiSI CCGG 7 cut(s) 260, 266, 306, 495, 1212, 1300, 2102
BslI CCNNNNNNNGG 3 cut(s) 306, 1168, 2108
BsmAI GTCTC 2 cut(s) 97, 1521
BsmBI CGTCTC 1 cut(s) 97
BsmI GAATGC 4 cut(s) 188, 673, 883, 1694
BsnI GGCC 7 cut(s) 211, 299, 992, 1002, 1145, 1215, 1261
Bso31I GGTCTC 1 cut(s) 1521
Bsp119I TTCGAA 2 cut(s) 1732, 1861
Bsp1286I GDGCHC 2 cut(s) 294, 808
Bsp13I TCCGGA 1 cut(s) 259
Bsp1407I TGTACA 1 cut(s) 1094
Bsp143I GATC 8 cut(s) 582, 771, 829, 1158, 1195, 1239, 1363, 1822
Bsp1720I GCTNAGC 1 cut(s) 621
BspANI GGCC 7 cut(s) 211, 299, 992, 1002, 1145, 1215, 1261
BspCNI CTCAG 4 cut(s) 634, 700, 1215, 1555
BspDI ATCGAT 1 cut(s) 1638
BspEI TCCGGA 1 cut(s) 259
BspFNI CGCG 1 cut(s) 1821
BspHI TCATGA 1 cut(s) 1510
BspLI GGNNCC 4 cut(s) 264, 1160, 1723, 1835
BspMI ACCTGC 2 cut(s) 505, 593
BspOI GCTAGC 1 cut(s) 1598
BspPI GGATC 2 cut(s) 1153, 1166
BspT104I TTCGAA 2 cut(s) 1732, 1861
BspTNI GGTCTC 1 cut(s) 1521
BsrDI GCAATG 1 cut(s) 1283
BsrFI RCCGGY 1 cut(s) 1211
BsrGI TGTACA 1 cut(s) 1094
BsrI ACTGG 4 cut(s) 212, 988, 1713, 2049
BssAI RCCGGY 1 cut(s) 1211
BssECI CCNNGG 2 cut(s) 799, 1167
BssMI GATC 8 cut(s) 582, 771, 829, 1158, 1195, 1239, 1363, 1822
BssNAI GTATAC 1 cut(s) 1401
BssNI GRCGYC 2 cut(s) 90, 752
BssSI CACGAG 2 cut(s) 315, 947
BssT1I CCWWGG 2 cut(s) 799, 1167
Bst1107I GTATAC 1 cut(s) 1401
Bst2BI CACGAG 2 cut(s) 315, 947
Bst2UI CCWGG 3 cut(s) 478, 1476, 1719
Bst4CI ACNGT 1 cut(s) 1905
Bst6I CTCTTC 3 cut(s) 546, 641, 1641
BstACI GRCGYC 2 cut(s) 90, 752
BstAPI GCANNNNNTGC 1 cut(s) 677
BstAUI TGTACA 1 cut(s) 1094
BstBI TTCGAA 2 cut(s) 1732, 1861
BstC8I GCNNGC 6 cut(s) 301, 790, 1105, 1213, 1596, 1686
BstDEI CTNAG 5 cut(s) 621, 708, 1202, 1542, 1577
BstENI CCTNNNNNAGG 1 cut(s) 1166
BstF5I GGATG 6 cut(s) 552, 858, 1190, 1484, 1537, 1663
BstFNI CGCG 1 cut(s) 1821
BstHHI GCGC 4 cut(s) 6, 305, 794, 1821
BstKTI GATC 8 cut(s) 585, 774, 832, 1161, 1198, 1242, 1366, 1825
BstMAI GTCTC 2 cut(s) 97, 1521
BstMBI GATC 8 cut(s) 582, 771, 829, 1158, 1195, 1239, 1363, 1822
BstMCI CGRYCG 2 cut(s) 1216, 1825
BstMWI GCNNNNNNNGC 9 cut(s) 104, 311, 320, 677, 686, 736, 1013, 1258, 1447
BstNI CCWGG 3 cut(s) 478, 1476, 1719
BstNSI RCATGY 2 cut(s) 1095, 1103
BstSCI CCNGG 6 cut(s) 264, 305, 476, 494, 1474, 1717
BstUI CGCG 1 cut(s) 1821
BstV1I GCAGC 5 cut(s) 748, 896, 1428, 1439, 1494
BstV2I GAAGAC 2 cut(s) 563, 1619
BstX2I RGATCY 1 cut(s) 1158
BstYI RGATCY 1 cut(s) 1158
BstZ17I GTATAC 1 cut(s) 1401
BstZI CGGCCG 1 cut(s) 1213
Bsu15I ATCGAT 1 cut(s) 1638
BsuRI GGCC 7 cut(s) 211, 299, 992, 1002, 1145, 1215, 1261
BsuTUI ATCGAT 1 cut(s) 1638
BtgZI GCGATG 2 cut(s) 55, 754
BtsCI GGATG 6 cut(s) 552, 858, 1190, 1484, 1537, 1663
BtsI GCAGTG 1 cut(s) 340
BtsIMutI CAGTG 4 cut(s) 340, 592, 781, 981
BveI ACCTGC 2 cut(s) 505, 593
Cac8I GCNNGC 6 cut(s) 301, 790, 1105, 1213, 1596, 1686
CaiI CAGNNNCTG 1 cut(s) 442
CciI TCATGA 1 cut(s) 1510
CfoI GCGC 4 cut(s) 6, 305, 794, 1821
Cfr10I RCCGGY 1 cut(s) 1211
Cfr13I GGNCC 1 cut(s) 262
ClaI ATCGAT 1 cut(s) 1638
CseI GACGC 2 cut(s) 79, 760
Csp6I GTAC 4 cut(s) 274, 1095, 1603, 1710
CviAII CATG 7 cut(s) 994, 1004, 1092, 1100, 1511, 1522, 2014
CviQI GTAC 4 cut(s) 274, 1095, 1603, 1710
DdeI CTNAG 5 cut(s) 621, 708, 1202, 1542, 1577
DpnI GATC 8 cut(s) 584, 773, 831, 1160, 1197, 1241, 1365, 1824
DpnII GATC 8 cut(s) 582, 771, 829, 1158, 1195, 1239, 1363, 1822
EaeI YGGCCR 5 cut(s) 209, 990, 1000, 1143, 1213
EagI CGGCCG 1 cut(s) 1213
Eam1104I CTCTTC 3 cut(s) 546, 641, 1641
EarI CTCTTC 3 cut(s) 546, 641, 1641
EciI GGCGGA 3 cut(s) 338, 859, 902
EclXI CGGCCG 1 cut(s) 1213
Eco130I CCWWGG 2 cut(s) 799, 1167
Eco147I AGGCCT 1 cut(s) 299
Eco31I GGTCTC 1 cut(s) 1521
Eco32I GATATC 1 cut(s) 963
Eco47I GGWCC 1 cut(s) 262
Eco52I CGGCCG 1 cut(s) 1213
Eco57I CTGAAG 1 cut(s) 1940
EcoNI CCTNNNNNAGG 1 cut(s) 1166
EcoRI GAATTC 1 cut(s) 388
EcoRII CCWGG 3 cut(s) 476, 1474, 1717
EcoRV GATATC 1 cut(s) 963
EcoT14I CCWWGG 2 cut(s) 799, 1167
EcoT22I ATGCAT 2 cut(s) 883, 1663
ErhI CCWWGG 2 cut(s) 799, 1167
Esp3I CGTCTC 1 cut(s) 97
FaeI CATG 7 cut(s) 997, 1007, 1095, 1103, 1514, 1525, 2017
FatI CATG 7 cut(s) 993, 1003, 1091, 1099, 1510, 1521, 2013
FauI CCCGC 2 cut(s) 122, 673
FauNDI CATATG 1 cut(s) 2093
FbaI TGATCA 3 cut(s) 582, 771, 1239
FblI GTMKAC 2 cut(s) 575, 1400
FokI GGATG 6 cut(s) 559, 865, 1197, 1491, 1524, 1670
FspBI CTAG 5 cut(s) 528, 1289, 1355, 1418, 1595
GlaI GCGC 4 cut(s) 5, 304, 793, 1820
GsaI CCCAGC 1 cut(s) 1602
HaeIII GGCC 7 cut(s) 211, 299, 992, 1002, 1145, 1215, 1261
HapII CCGG 7 cut(s) 260, 266, 306, 495, 1212, 1300, 2102
HgaI GACGC 2 cut(s) 79, 760
HhaI GCGC 4 cut(s) 6, 305, 794, 1821
Hin1I GRCGYC 2 cut(s) 90, 752
Hin1II CATG 7 cut(s) 997, 1007, 1095, 1103, 1514, 1525, 2017
Hin6I GCGC 4 cut(s) 4, 303, 792, 1819
HinP1I GCGC 4 cut(s) 4, 303, 792, 1819
HincII GTYRAC 2 cut(s) 576, 1086
HindII GTYRAC 2 cut(s) 576, 1086
HindIII AAGCTT 2 cut(s) 1014, 1930
HinfI GANTC 4 cut(s) 368, 1358, 1734, 1878
HpaII CCGG 7 cut(s) 260, 266, 306, 495, 1212, 1300, 2102
HphI GGTGA 5 cut(s) 617, 760, 788, 1996, 2116
Hpy166II GTNNAC 8 cut(s) 276, 340, 576, 1086, 1097, 1401, 1498, 1672
Hpy188I TCNGA 5 cut(s) 720, 1363, 1545, 1910, 2021
Hpy188III TCNNGA 7 cut(s) 260, 809, 1349, 1371, 1511, 1738, 1760
Hpy8I GTNNAC 8 cut(s) 276, 340, 576, 1086, 1097, 1401, 1498, 1672
HpyAV CCTTC 5 cut(s) 188, 289, 517, 592, 1172
HpyCH4III ACNGT 1 cut(s) 1905
HpyCH4IV ACGT 2 cut(s) 1330, 1403
HpyF10VI GCNNNNNNNGC 9 cut(s) 104, 311, 320, 677, 686, 736, 1013, 1258, 1447
HpyF3I CTNAG 5 cut(s) 621, 708, 1202, 1542, 1577
HpySE526I ACGT 2 cut(s) 1330, 1403
Hsp92I GRCGYC 2 cut(s) 90, 752
Hsp92II CATG 7 cut(s) 997, 1007, 1095, 1103, 1514, 1525, 2017
HspAI GCGC 4 cut(s) 4, 303, 792, 1819
Kpn2I TCCGGA 1 cut(s) 259
KroI GCCGGC 1 cut(s) 1211
KroNI GCCGGC 1 cut(s) 1213
Ksp22I TGATCA 3 cut(s) 582, 771, 1239
Kzo9I GATC 8 cut(s) 582, 771, 829, 1158, 1195, 1239, 1363, 1822
LmnI GCTCC 3 cut(s) 262, 969, 1280
Lsp1109I GCAGC 5 cut(s) 748, 896, 1428, 1439, 1494
LweI GCATC 9 cut(s) 244, 630, 933, 1245, 1414, 1469, 1546, 1648, 1762
MaeI CTAG 5 cut(s) 528, 1289, 1355, 1418, 1595
MaeII ACGT 2 cut(s) 1330, 1403
MaeIII GTNAC 5 cut(s) 167, 748, 763, 1757, 1946
MalI GATC 8 cut(s) 584, 773, 831, 1160, 1197, 1241, 1365, 1824
MboI GATC 8 cut(s) 582, 771, 829, 1158, 1195, 1239, 1363, 1822
MfeI CAATTG 1 cut(s) 239
MflI RGATCY 1 cut(s) 1158
MhlI GDGCHC 2 cut(s) 294, 808
MlsI TGGCCA 4 cut(s) 211, 992, 1002, 1145
MluNI TGGCCA 4 cut(s) 211, 992, 1002, 1145
MmeI TCCRAC 1 cut(s) 591
Mox20I TGGCCA 4 cut(s) 211, 992, 1002, 1145
Mph1103I ATGCAT 2 cut(s) 883, 1663
MroI TCCGGA 1 cut(s) 259
MroNI GCCGGC 1 cut(s) 1211
MroXI GAANNNNTTC 2 cut(s) 161, 1893
MscI TGGCCA 4 cut(s) 211, 992, 1002, 1145
MseI TTAA 3 cut(s) 135, 936, 1784
MslI CAYNNNNRTG 5 cut(s) 287, 801, 1008, 1073, 1477
Msp20I TGGCCA 4 cut(s) 211, 992, 1002, 1145
MspA1I CMGCKG 2 cut(s) 625, 1485
MspI CCGG 7 cut(s) 260, 266, 306, 495, 1212, 1300, 2102
MspR9I CCNGG 6 cut(s) 266, 307, 478, 496, 1476, 1719
MunI CAATTG 1 cut(s) 239
Mva1269I GAATGC 4 cut(s) 188, 673, 883, 1694
MvaI CCWGG 3 cut(s) 478, 1476, 1719
MvnI CGCG 1 cut(s) 1821
MwoI GCNNNNNNNGC 9 cut(s) 104, 311, 320, 677, 686, 736, 1013, 1258, 1447
NaeI GCCGGC 1 cut(s) 1213
NciI CCSGG 3 cut(s) 266, 307, 496
NdeI CATATG 1 cut(s) 2093
NdeII GATC 8 cut(s) 582, 771, 829, 1158, 1195, 1239, 1363, 1822
NgoMIV GCCGGC 1 cut(s) 1211
NheI GCTAGC 1 cut(s) 1594
NlaIII CATG 7 cut(s) 997, 1007, 1095, 1103, 1514, 1525, 2017
NlaIV GGNNCC 4 cut(s) 264, 1160, 1723, 1835
NmuCI GTSAC 3 cut(s) 748, 1757, 1946
NsiI ATGCAT 2 cut(s) 883, 1663
NspI RCATGY 2 cut(s) 1095, 1103
NspV TTCGAA 2 cut(s) 1732, 1861
OliI CACNNNNGTG 2 cut(s) 287, 801
PagI TCATGA 1 cut(s) 1510
PaqCI CACCTGC 1 cut(s) 593
PceI AGGCCT 1 cut(s) 299
PcsI WCGNNNNNNNCGW 3 cut(s) 126, 1026, 1767
PctI GAATGC 4 cut(s) 188, 673, 883, 1694
PdiI GCCGGC 1 cut(s) 1213
PdmI GAANNNNTTC 2 cut(s) 161, 1893
PfeI GAWTC 4 cut(s) 368, 1358, 1734, 1878
PfoI TCCNGGA 2 cut(s) 476, 1717
Ple19I CGATCG 1 cut(s) 1825
PshBI ATTAAT 1 cut(s) 1784
PsiI TTATAA 2 cut(s) 839, 1308
Psp6I CCWGG 3 cut(s) 476, 1474, 1717
PspFI CCCAGC 1 cut(s) 1598
PspGI CCWGG 3 cut(s) 476, 1474, 1717
PspN4I GGNNCC 4 cut(s) 264, 1160, 1723, 1835
PspPI GGNCC 1 cut(s) 262
PstNI CAGNNNCTG 1 cut(s) 442
PsuI RGATCY 1 cut(s) 1158
PvuI CGATCG 1 cut(s) 1825
PvuII CAGCTG 1 cut(s) 1485
RgaI GCGATCGC 1 cut(s) 1825
RsaI GTAC 4 cut(s) 275, 1096, 1604, 1711
RsaNI GTAC 4 cut(s) 274, 1095, 1603, 1710
RseI CAYNNNNRTG 5 cut(s) 287, 801, 1008, 1073, 1477
SalI GTCGAC 1 cut(s) 574
SaqAI TTAA 3 cut(s) 135, 936, 1784
Sau3AI GATC 8 cut(s) 582, 771, 829, 1158, 1195, 1239, 1363, 1822
Sau96I GGNCC 1 cut(s) 262
ScrFI CCNGG 6 cut(s) 266, 307, 478, 496, 1476, 1719
SduI GDGCHC 2 cut(s) 294, 808
SfaAI GCGATCGC 1 cut(s) 1825
SfaNI GCATC 9 cut(s) 244, 630, 933, 1245, 1414, 1469, 1546, 1648, 1762
SfuI TTCGAA 2 cut(s) 1732, 1861
SgfI GCGATCGC 1 cut(s) 1825
SinI GGWCC 1 cut(s) 262
SmiMI CAYNNNNRTG 5 cut(s) 287, 801, 1008, 1073, 1477
SmlI CTYRAG 2 cut(s) 293, 1124
SmoI CTYRAG 2 cut(s) 293, 1124
SseBI AGGCCT 1 cut(s) 299
SspI AATATT 1 cut(s) 160
SspMI CTAG 5 cut(s) 528, 1289, 1355, 1418, 1595
StuI AGGCCT 1 cut(s) 299
StyD4I CCNGG 6 cut(s) 264, 305, 476, 494, 1474, 1717
StyI CCWWGG 2 cut(s) 799, 1167
TaaI ACNGT 1 cut(s) 1905
TaiI ACGT 2 cut(s) 1333, 1406
TatI WGTACW 2 cut(s) 273, 1094
TauI GCSGC 5 cut(s) 101, 628, 909, 1218, 1261
TfiI GAWTC 4 cut(s) 368, 1358, 1734, 1878
Tru1I TTAA 3 cut(s) 135, 936, 1784
Tru9I TTAA 3 cut(s) 135, 936, 1784
TscAI CASTG 4 cut(s) 347, 592, 781, 988
TseFI GTSAC 3 cut(s) 748, 1757, 1946
TseI GCWGC 5 cut(s) 736, 909, 1427, 1441, 1482
Tsp45I GTSAC 3 cut(s) 748, 1757, 1946
TspDTI ATGAA 7 cut(s) 1010, 1026, 1499, 1510, 1604, 1656, 1983
TspGWI ACGGA 1 cut(s) 1631
TspRI CASTG 4 cut(s) 347, 592, 781, 988
VpaK11BI GGWCC 1 cut(s) 262
VspI ATTAAT 1 cut(s) 1784
XagI CCTNNNNNAGG 1 cut(s) 1166
XapI RAATTY 4 cut(s) 388, 1744, 1856, 1864
XceI RCATGY 2 cut(s) 1095, 1103
XcmI CCANNNNNNNNNTGG 1 cut(s) 1847
XmiI GTMKAC 2 cut(s) 575, 1400
XmnI GAANNNNTTC 2 cut(s) 161, 1893
XspI CTAG 5 cut(s) 528, 1289, 1355, 1418, 1595
Zsp2I ATGCAT 2 cut(s) 883, 1663
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.