Rw5G027000

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0017629
Physical Location & Seq
Forward (+)
39582662 .. 39584691
2030 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G027000.1

Sequence Viewer

Length: 1881 bp
ATGCGCCACCTCTACCAAATCCAATCCCAGCTCACCACCAACCCCATCCCCTCCATCGACCCAAACATCATTGCCGTCAAGCTCATCGGCGCGTTCGCCCATTTGGCCAACACGCGCCACTTGGCCCTCATATTCCGCCACTATCTCCAAACCCCAAACATCTTCGCCTACAATGCCCTTCTCAAGGCCTTTGCTCAGAACAATGACTGGCACCACACCATTCTCTACTTCAATTCCCAATTGCTTTCGCTGAATGCTCCGCGCCCGGATGAGTACACCTTCACCTCTGTGCTCAAGGCCTGCGCCGGCCTCGCCCGAGTGACTGACGGAGAGAAAGTCCACTGCCTTGTCACTAAATTCGGGTGCGAGGGGAATCTCTTTGTCAGAAATTCGCTGACTGACATGTACTTCAAGTTTGGGAGGTTTGGGATTGCGCAGAAGCTGTTCGATGAAATGCGTGTGAGAGATGTCGTTTCGTGGAACACTTTGGTTGCTGGGTACTGCGTGAGTGGGGAGGTTGGTGAGGCTAGGAGGGTGTTTGACGGCATGGTAGAGAAGACTTTGTTTTCTTGGTCGACTATGATCAGTGCGTATGCCAAGTTGGGTGAGTTGGAGGAAGCGAGGCGGCTTTTTAATGAGATGCCGCAGAGGAATGTGGTTTCGTGGAATGCGATGATTGCTGGGTATGCGCAGAATGAGAAGTATGCTGAGGCTGTTGGGTTGTTTAGGGAAATGCAAGAGTGTGGGTTGGCGCCAAATGATGTTACGCTTGTTAGTGTTTTGTCTGCCTGCGCTCATCTTGGGGCACTTGATTTGGGGAAGTGGATTGATAGGTTTATAAAGCGAAGCGGGATGGAGTTGGGTCTGTTTTTGGGGAATGCTTTAGCGGATATGTATGCCAAGTGTGGCTGCATAACCGAAGCTAGGCTGGTTTTCAATAACATGCATGAGAGGGATGTGATATCTTGGAGTATTATCATTACTGGGTTGGCCATGAACGGGCATGCTGATGAAGCTTTCGAGTGTTTTAGTAAGATGATTGAACACGGTTTGAAGCCAAATGAAATTACGTTTATGGGGTTATTGACAGCATGTACTCATGCTGGTTTGGTAGACAAGGGGCTTGAGTATTTTGATATGATGGAGAAAGCATATGGAATATCTCCCAAGATTGAGCACTATGGGTGTTTGGTTGATCTTCTCAGCCGTGCCGGTCGCCTTGCTAAAGCAGAGGATATGATCAATTCCATGCCTATGAAGCCTAATGTTATAGTCTGGGGTGCATTGCTCGGTGGTTGTCGGATTTATAAAGATACTGATAGAGGGGAGCGTGTTGTTCAGCAGATTCTTGAACTGGATTCTGAGCACTCTGGAAGCTATGTTTATCTTGCAAATCTGTATGCTTCGATGGGAAGGCTTGATGATGCAGCACAGTGTAGACTGAGAATGCGAGATAAAGGCATAACAAAAACACCTGGCAGCAGTTGGATAGAGGTGGATAACATGGTTCATGAATTCTTCATGGGAGATTTATCGCATCCTCAGTTGGACAAAATATATTCAATGATTAAAGAACTGAGGTGGAAAATGAAGCTAGCCGGATACAAACCAAAGACAGATCTGGTGCTTCATAACATAGACGAAGAAGAGAAAGAGGATGCTTTATCTGTTCATAGTGAGAGGCTGGCCATTGCATTTGGGCTTATCAGTACTAGCCCAGGAACTACAATTCGAATTGTAAAGAACTTGCGAGTTTGTAGTGACTGTCATGACGCGACAAAATTAATCTCCAAAATTGTAGAACGAGAGATTATCGTACGAGACCGAAGTCGCTTCCATCATTTCAAAGATGGTAAATGTTCTTGCAATGACTACTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

626

Amino Acids

70.75

Weight (kDa)

6.73

Isoelectric Point (pI)

34.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 155 - 186 5e-06 PPR repeat family
PPR_1 PF12854 155 - 184 5.3e-07 PPR repeat
PPR PF01535 158 - 186 2.8e-06 PPR repeat
PPR_2 PF13041 188 - 219 5.8e-07 PPR repeat family
PPR PF01535 189 - 219 3e-09 PPR repeat
PPR_2 PF13041 217 - 266 2.8e-13 PPR repeat family
PPR PF01535 220 - 250 4.8e-09 PPR repeat
PPR_2 PF13041 318 - 366 2.2e-11 PPR repeat family
PPR PF01535 321 - 351 3.1e-06 PPR repeat
E_motif PF20431 436 - 498 2e-16 E motif
DYW_deaminase PF14432 534 - 626 8.7e-44 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017629)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g24230
malus_domestica MD15G1089600.v1.1
prunus_persica Prupe.1G444200_v2.0.a1
pyrus_communis pycom15g08400
rosa_chinensis RchiOBHm_Chr5g0043351
rosa_laevigata RLG00000034182
rosa_multiflora Rmu_sc0000060.1_g000011 Rmu_sc0000060.1_g000039
rosa_roxburghii Rroxscaffold_1G00037500
rosa_rugosa Rorug05G0205400
rosa_samantha Rh5CG327400
rosa_wichuraiana Rw5G027000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 839, 1308
Acc16I TGCGCA 2 cut(s) 435, 690
AccB1I GGYRCC 2 cut(s) 210, 751
AccI GTMKAC 3 cut(s) 575, 1113, 1438
AccII CGCG 4 cut(s) 92, 115, 262, 1775
AciI CCGC 6 cut(s) 136, 260, 625, 644, 849, 887
AcoI YGGCCR 3 cut(s) 105, 990, 1686
AcsI RAATTY 3 cut(s) 356, 388, 1514
AcyI GRCGYC 1 cut(s) 752
AfaI GTAC 6 cut(s) 275, 407, 500, 1096, 1711, 1818
AfiI CCNNNNNNNGG 3 cut(s) 184, 924, 999
AflIII ACRYGT 1 cut(s) 402
AgsI TTSAA 8 cut(s) 232, 412, 937, 1043, 1054, 1352, 1563, 1846
AjnI CCWGG 2 cut(s) 1474, 1717
AleI CACNNNNGTG 1 cut(s) 287
AluBI AGCT 7 cut(s) 31, 82, 442, 923, 1016, 1377, 1594
AluI AGCT 7 cut(s) 31, 82, 442, 923, 1016, 1377, 1594
Alw21I GWGCWC 3 cut(s) 294, 1179, 1368
Alw26I GTCTC 1 cut(s) 1815
AlwNI CAGNNNCTG 1 cut(s) 442
Ama87I CYCGRG 1 cut(s) 315
AoxI GGCC 7 cut(s) 105, 123, 186, 297, 307, 990, 1686
ApeKI GCWGC 3 cut(s) 909, 1427, 1479
ApoI RAATTY 3 cut(s) 356, 388, 1514
AseI ATTAAT 1 cut(s) 1784
Asp700I GAANNNNTTC 1 cut(s) 443
AspLEI GCGC 9 cut(s) 6, 92, 117, 264, 305, 436, 691, 754, 794
AspS9I GGNCC 1 cut(s) 124
AsuC2I CCSGG 1 cut(s) 266
AsuHPI GGTGA 4 cut(s) 25, 274, 533, 617
AsuII TTCGAA 1 cut(s) 1732
AsuNHI GCTAGC 1 cut(s) 1594
AvaI CYCGRG 1 cut(s) 315
BaeGI GKGCMC 1 cut(s) 808
BalI TGGCCA 3 cut(s) 107, 992, 1688
BanI GGYRCC 2 cut(s) 210, 751
BbsI GAAGAC 1 cut(s) 563
Bbv12I GWGCWC 3 cut(s) 294, 1179, 1368
BbvCI CCTCAGC 1 cut(s) 708
BbvI GCAGC 3 cut(s) 896, 1439, 1491
BccI CCATC 7 cut(s) 53, 62, 847, 1135, 1402, 1844, 1845
BceAI ACGGC 3 cut(s) 59, 559, 1191
BciT130I CCWGG 2 cut(s) 1476, 1719
BciVI GTATCC 1 cut(s) 1595
BclI TGATCA 2 cut(s) 582, 1239
BcnI CCSGG 1 cut(s) 266
BcoDI GTCTC 1 cut(s) 1815
BfaI CTAG 4 cut(s) 528, 924, 1595, 1713
BfoI RGCGCY 1 cut(s) 755
BfuI GTATCC 1 cut(s) 1595
BglI GCCNNNNNGGC 1 cut(s) 104
BglII AGATCT 1 cut(s) 1618
BisI GCNGC 5 cut(s) 626, 644, 910, 1428, 1480
BlsI GCNGC 5 cut(s) 627, 645, 911, 1429, 1481
BmcAI AGTACT 1 cut(s) 1711
Bme1390I CCNGG 3 cut(s) 266, 1476, 1719
BmeT110I CYCGRG 1 cut(s) 315
BmgT120I GGNCC 1 cut(s) 124
BmiI GGNNCC 2 cut(s) 212, 753
BmrFI CCNGG 3 cut(s) 266, 1476, 1719
BmrI ACTGGG 1 cut(s) 993
BmsI GCATC 4 cut(s) 630, 1414, 1546, 1648
BmtI GCTAGC 1 cut(s) 1598
BmuI ACTGGG 1 cut(s) 993
BoxI GACNNNNGTC 1 cut(s) 1827
BpiI GAAGAC 1 cut(s) 563
Bpu10I CCTNAGC 1 cut(s) 708
Bpu14I TTCGAA 1 cut(s) 1732
BpuEI CTTGAG 3 cut(s) 167, 278, 1145
BpuMI CCSGG 1 cut(s) 266
BsaHI GRCGYC 1 cut(s) 752
BsaI GGTCTC 1 cut(s) 1815
BsaJI CCNNGG 1 cut(s) 1717
BsaXI ACNNNNNCTCC 2 cut(s) 321, 351
Bsc4I CCNNNNNNNGG 3 cut(s) 184, 924, 999
Bse118I RCCGGY 2 cut(s) 305, 1211
Bse1I ACTGG 4 cut(s) 212, 988, 1359, 1880
Bse3DI GCAATG 4 cut(s) 69, 1283, 1689, 1873
BseBI CCWGG 2 cut(s) 1476, 1719
BseDI CCNNGG 1 cut(s) 1717
BseGI GGATG 6 cut(s) 45, 274, 858, 961, 1537, 1663
BseLI CCNNNNNNNGG 3 cut(s) 184, 924, 999
BseMI GCAATG 4 cut(s) 69, 1283, 1689, 1873
BseMII CTCAG 7 cut(s) 209, 699, 1216, 1353, 1433, 1556, 1568
BseNI ACTGG 4 cut(s) 212, 988, 1359, 1880
BseSI GKGCMC 1 cut(s) 808
BseXI GCAGC 3 cut(s) 896, 1439, 1491
BseYI CCCAGC 3 cut(s) 27, 494, 680
Bsh1236I CGCG 4 cut(s) 92, 115, 262, 1775
Bsh1285I CGRYCG 1 cut(s) 1216
BshFI GGCC 7 cut(s) 107, 125, 188, 299, 309, 992, 1688
BshNI GGYRCC 2 cut(s) 210, 751
BsiEI CGRYCG 1 cut(s) 1216
BsiHKAI GWGCWC 3 cut(s) 294, 1179, 1368
BsiHKCI CYCGRG 1 cut(s) 315
BsiSI CCGG 4 cut(s) 266, 306, 1212, 1599
BsiWI CGTACG 1 cut(s) 1816
BslI CCNNNNNNNGG 3 cut(s) 184, 924, 999
BsmAI GTCTC 1 cut(s) 1815
BsmI GAATGC 4 cut(s) 259, 673, 883, 1452
BsnI GGCC 7 cut(s) 107, 125, 188, 299, 309, 992, 1688
Bso31I GGTCTC 1 cut(s) 1815
BsoBI CYCGRG 1 cut(s) 315
Bsp119I TTCGAA 1 cut(s) 1732
Bsp1286I GDGCHC 4 cut(s) 294, 808, 1179, 1368
Bsp143I GATC 4 cut(s) 582, 1195, 1239, 1618
BspACI CCGC 6 cut(s) 136, 260, 625, 644, 849, 887
BspANI GGCC 7 cut(s) 107, 125, 188, 299, 309, 992, 1688
BspCNI CTCAG 7 cut(s) 208, 700, 1215, 1354, 1434, 1555, 1569
BspFNI CGCG 4 cut(s) 92, 115, 262, 1775
BspHI TCATGA 2 cut(s) 1510, 1768
BspLI GGNNCC 2 cut(s) 212, 753
BspOI GCTAGC 1 cut(s) 1598
BspT104I TTCGAA 1 cut(s) 1732
BspT107I GGYRCC 2 cut(s) 210, 751
BspTNI GGTCTC 1 cut(s) 1815
BsrDI GCAATG 4 cut(s) 69, 1283, 1689, 1873
BsrFI RCCGGY 2 cut(s) 305, 1211
BsrI ACTGG 4 cut(s) 212, 988, 1359, 1880
BssAI RCCGGY 2 cut(s) 305, 1211
BssECI CCNNGG 1 cut(s) 1717
BssMI GATC 4 cut(s) 582, 1195, 1239, 1618
BssNI GRCGYC 1 cut(s) 752
Bst2UI CCWGG 2 cut(s) 1476, 1719
Bst4CI ACNGT 3 cut(s) 1049, 1434, 1766
Bst6I CTCTTC 1 cut(s) 1641
BstACI GRCGYC 1 cut(s) 752
BstBI TTCGAA 1 cut(s) 1732
BstC8I GCNNGC 6 cut(s) 301, 307, 790, 1005, 1596, 1686
BstDEI CTNAG 7 cut(s) 195, 708, 1202, 1362, 1442, 1542, 1577
BstENI CCTNNNNNAGG 1 cut(s) 182
BstF5I GGATG 6 cut(s) 45, 274, 858, 961, 1537, 1663
BstFNI CGCG 4 cut(s) 92, 115, 262, 1775
BstH2I RGCGCY 1 cut(s) 755
BstHHI GCGC 9 cut(s) 6, 92, 117, 264, 305, 436, 691, 754, 794
BstKTI GATC 4 cut(s) 585, 1198, 1242, 1621
BstMAI GTCTC 1 cut(s) 1815
BstMBI GATC 4 cut(s) 582, 1195, 1239, 1618
BstMCI CGRYCG 1 cut(s) 1216
BstMWI GCNNNNNNNGC 7 cut(s) 104, 173, 311, 677, 686, 1013, 1258
BstNI CCWGG 2 cut(s) 1476, 1719
BstNSI RCATGY 4 cut(s) 406, 946, 1007, 1095
BstPAI GACNNNNGTC 1 cut(s) 1827
BstSCI CCNGG 3 cut(s) 264, 1474, 1717
BstSLI GKGCMC 1 cut(s) 808
BstUI CGCG 4 cut(s) 92, 115, 262, 1775
BstV1I GCAGC 3 cut(s) 896, 1439, 1491
BstV2I GAAGAC 1 cut(s) 563
BstX2I RGATCY 1 cut(s) 1618
BstYI RGATCY 1 cut(s) 1618
BsuI GTATCC 1 cut(s) 1595
BsuRI GGCC 7 cut(s) 107, 125, 188, 299, 309, 992, 1688
BtgZI GCGATG 1 cut(s) 686
BtsCI GGATG 6 cut(s) 45, 274, 858, 961, 1537, 1663
BtsI GCAGTG 1 cut(s) 340
BtsIMutI CAGTG 3 cut(s) 340, 592, 1439
Cac8I GCNNGC 6 cut(s) 301, 307, 790, 1005, 1596, 1686
CaiI CAGNNNCTG 1 cut(s) 442
CciI TCATGA 2 cut(s) 1510, 1768
CfoI GCGC 9 cut(s) 6, 92, 117, 264, 305, 436, 691, 754, 794
Cfr10I RCCGGY 2 cut(s) 305, 1211
Cfr13I GGNCC 1 cut(s) 124
CseI GACGC 1 cut(s) 1781
Csp6I GTAC 6 cut(s) 274, 406, 499, 1095, 1710, 1817
CviQI GTAC 6 cut(s) 274, 406, 499, 1095, 1710, 1817
DdeI CTNAG 7 cut(s) 195, 708, 1202, 1362, 1442, 1542, 1577
DinI GGCGCC 1 cut(s) 753
DpnI GATC 4 cut(s) 584, 1197, 1241, 1620
DpnII GATC 4 cut(s) 582, 1195, 1239, 1618
EaeI YGGCCR 3 cut(s) 105, 990, 1686
Eam1104I CTCTTC 1 cut(s) 1641
EarI CTCTTC 1 cut(s) 1641
EciI GGCGGA 1 cut(s) 125
Eco147I AGGCCT 2 cut(s) 188, 299
Eco31I GGTCTC 1 cut(s) 1815
Eco32I GATATC 1 cut(s) 963
Eco88I CYCGRG 1 cut(s) 315
EcoNI CCTNNNNNAGG 1 cut(s) 182
EcoRI GAATTC 1 cut(s) 1514
EcoRII CCWGG 2 cut(s) 1474, 1717
EcoRV GATATC 1 cut(s) 963
EcoT22I ATGCAT 1 cut(s) 948
EgeI GGCGCC 1 cut(s) 753
EheI GGCGCC 1 cut(s) 753
FauI CCCGC 1 cut(s) 842
FauNDI CATATG 1 cut(s) 1153
FbaI TGATCA 2 cut(s) 582, 1239
FblI GTMKAC 3 cut(s) 575, 1113, 1438
Fnu4HI GCNGC 5 cut(s) 626, 644, 910, 1428, 1480
FokI GGATG 6 cut(s) 32, 281, 865, 968, 1524, 1670
Fsp4HI GCNGC 5 cut(s) 626, 644, 910, 1428, 1480
FspBI CTAG 4 cut(s) 528, 924, 1595, 1713
FspI TGCGCA 2 cut(s) 435, 690
GlaI GCGC 9 cut(s) 5, 91, 116, 263, 304, 435, 690, 753, 793
GluI GCNGC 5 cut(s) 626, 644, 910, 1428, 1480
GsaI CCCAGC 3 cut(s) 31, 498, 684
HaeII RGCGCY 1 cut(s) 755
HaeIII GGCC 7 cut(s) 107, 125, 188, 299, 309, 992, 1688
HapII CCGG 4 cut(s) 266, 306, 1212, 1599
HgaI GACGC 1 cut(s) 1781
HhaI GCGC 9 cut(s) 6, 92, 117, 264, 305, 436, 691, 754, 794
Hin1I GRCGYC 1 cut(s) 752
Hin6I GCGC 9 cut(s) 4, 90, 115, 262, 303, 434, 689, 752, 792
HinP1I GCGC 9 cut(s) 4, 90, 115, 262, 303, 434, 689, 752, 792
HincII GTYRAC 1 cut(s) 576
HindII GTYRAC 1 cut(s) 576
HindIII AAGCTT 1 cut(s) 1014
HinfI GANTC 3 cut(s) 373, 1345, 1358
HpaII CCGG 4 cut(s) 266, 306, 1212, 1599
HphI GGTGA 4 cut(s) 25, 274, 533, 617
Hpy166II GTNNAC 5 cut(s) 276, 340, 576, 1114, 1439
Hpy188I TCNGA 4 cut(s) 198, 386, 1302, 1363
Hpy188III TCNNGA 4 cut(s) 1349, 1371, 1511, 1769
Hpy8I GTNNAC 5 cut(s) 276, 340, 576, 1114, 1439
HpyAV CCTTC 3 cut(s) 188, 289, 1407
HpyCH4III ACNGT 3 cut(s) 1049, 1434, 1766
HpyCH4IV ACGT 1 cut(s) 1070
HpyCH4V TGCA 8 cut(s) 736, 912, 946, 1283, 1391, 1427, 1694, 1866
HpyF10VI GCNNNNNNNGC 7 cut(s) 104, 173, 311, 677, 686, 1013, 1258
HpyF3I CTNAG 7 cut(s) 195, 708, 1202, 1362, 1442, 1542, 1577
HpySE526I ACGT 1 cut(s) 1070
Hsp92I GRCGYC 1 cut(s) 752
HspAI GCGC 9 cut(s) 4, 90, 115, 262, 303, 434, 689, 752, 792
KasI GGCGCC 1 cut(s) 751
KroI GCCGGC 1 cut(s) 305
KroNI GCCGGC 1 cut(s) 307
Ksp22I TGATCA 2 cut(s) 582, 1239
Kzo9I GATC 4 cut(s) 582, 1195, 1239, 1618
LmnI GCTCC 2 cut(s) 262, 1327
Lsp1109I GCAGC 3 cut(s) 896, 1439, 1491
LweI GCATC 4 cut(s) 630, 1414, 1546, 1648
MaeI CTAG 4 cut(s) 528, 924, 1595, 1713
MaeII ACGT 1 cut(s) 1070
MaeIII GTNAC 4 cut(s) 319, 349, 763, 1760
MalI GATC 4 cut(s) 584, 1197, 1241, 1620
MboI GATC 4 cut(s) 582, 1195, 1239, 1618
MboII GAAGA 6 cut(s) 154, 568, 1190, 1510, 1655, 1658
MfeI CAATTG 1 cut(s) 239
MflI RGATCY 1 cut(s) 1618
MhlI GDGCHC 4 cut(s) 294, 808, 1179, 1368
MlsI TGGCCA 3 cut(s) 107, 992, 1688
MluNI TGGCCA 3 cut(s) 107, 992, 1688
Mly113I GGCGCC 1 cut(s) 752
MmeI TCCRAC 4 cut(s) 591, 1280, 1466, 1527
Mox20I TGGCCA 3 cut(s) 107, 992, 1688
Mph1103I ATGCAT 1 cut(s) 948
MroNI GCCGGC 1 cut(s) 305
MroXI GAANNNNTTC 1 cut(s) 443
MscI TGGCCA 3 cut(s) 107, 992, 1688
MseI TTAA 3 cut(s) 633, 1569, 1784
MslI CAYNNNNRTG 3 cut(s) 287, 1008, 1253
Msp20I TGGCCA 3 cut(s) 107, 992, 1688
MspI CCGG 4 cut(s) 266, 306, 1212, 1599
MspR9I CCNGG 3 cut(s) 266, 1476, 1719
MunI CAATTG 1 cut(s) 239
Mva1269I GAATGC 4 cut(s) 259, 673, 883, 1452
MvaI CCWGG 2 cut(s) 1476, 1719
MvnI CGCG 4 cut(s) 92, 115, 262, 1775
MwoI GCNNNNNNNGC 7 cut(s) 104, 173, 311, 677, 686, 1013, 1258
NaeI GCCGGC 1 cut(s) 307
NarI GGCGCC 1 cut(s) 752
NciI CCSGG 1 cut(s) 266
NdeI CATATG 1 cut(s) 1153
NdeII GATC 4 cut(s) 582, 1195, 1239, 1618
NgoMIV GCCGGC 1 cut(s) 305
NheI GCTAGC 1 cut(s) 1594
NlaIV GGNNCC 2 cut(s) 212, 753
NmuCI GTSAC 3 cut(s) 319, 349, 1760
NsbI TGCGCA 2 cut(s) 435, 690
NsiI ATGCAT 1 cut(s) 948
NspI RCATGY 4 cut(s) 406, 946, 1007, 1095
NspV TTCGAA 1 cut(s) 1732
OliI CACNNNNGTG 1 cut(s) 287
PaeI GCATGC 1 cut(s) 1007
PagI TCATGA 2 cut(s) 1510, 1768
PceI AGGCCT 2 cut(s) 188, 299
PciI ACATGT 1 cut(s) 402
PcsI WCGNNNNNNNCGW 1 cut(s) 668
PctI GAATGC 4 cut(s) 259, 673, 883, 1452
PdiI GCCGGC 1 cut(s) 307
PdmI GAANNNNTTC 1 cut(s) 443
PfeI GAWTC 3 cut(s) 373, 1345, 1358
Pfl23II CGTACG 1 cut(s) 1816
PkrI GCNGC 5 cut(s) 627, 645, 911, 1429, 1481
PluTI GGCGCC 1 cut(s) 755
PscI ACATGT 1 cut(s) 402
PshAI GACNNNNGTC 1 cut(s) 1827
PshBI ATTAAT 1 cut(s) 1784
PsiI TTATAA 2 cut(s) 839, 1308
Psp6I CCWGG 2 cut(s) 1474, 1717
PspFI CCCAGC 3 cut(s) 27, 494, 680
PspGI CCWGG 2 cut(s) 1474, 1717
PspLI CGTACG 1 cut(s) 1816
PspN4I GGNNCC 2 cut(s) 212, 753
PspPI GGNCC 1 cut(s) 124
PstNI CAGNNNCTG 1 cut(s) 442
PsuI RGATCY 1 cut(s) 1618
RsaI GTAC 6 cut(s) 275, 407, 500, 1096, 1711, 1818
RsaNI GTAC 6 cut(s) 274, 406, 499, 1095, 1710, 1817
RseI CAYNNNNRTG 3 cut(s) 287, 1008, 1253
SalI GTCGAC 1 cut(s) 574
SaqAI TTAA 3 cut(s) 633, 1569, 1784
SatI GCNGC 5 cut(s) 626, 644, 910, 1428, 1480
Sau3AI GATC 4 cut(s) 582, 1195, 1239, 1618
Sau96I GGNCC 1 cut(s) 124
ScaI AGTACT 1 cut(s) 1711
ScrFI CCNGG 3 cut(s) 266, 1476, 1719
SduI GDGCHC 4 cut(s) 294, 808, 1179, 1368
SfaNI GCATC 4 cut(s) 630, 1414, 1546, 1648
SfoI GGCGCC 1 cut(s) 753
SfuI TTCGAA 1 cut(s) 1732
SmiMI CAYNNNNRTG 3 cut(s) 287, 1008, 1253
SmlI CTYRAG 3 cut(s) 182, 293, 1124
SmoI CTYRAG 3 cut(s) 182, 293, 1124
SphI GCATGC 1 cut(s) 1007
SseBI AGGCCT 2 cut(s) 188, 299
SsiI CCGC 6 cut(s) 136, 260, 625, 644, 849, 887
SspDI GGCGCC 1 cut(s) 751
SspMI CTAG 4 cut(s) 528, 924, 1595, 1713
StuI AGGCCT 2 cut(s) 188, 299
StyD4I CCNGG 3 cut(s) 264, 1474, 1717
TaaI ACNGT 3 cut(s) 1049, 1434, 1766
TaiI ACGT 1 cut(s) 1073
TaqI TCGA 6 cut(s) 57, 447, 575, 1020, 1406, 1732
TaqII GACCGA 1 cut(s) 1839
TatI WGTACW 4 cut(s) 273, 405, 1094, 1709
TauI GCSGC 2 cut(s) 628, 646
TfiI GAWTC 3 cut(s) 373, 1345, 1358
Tru1I TTAA 3 cut(s) 633, 1569, 1784
Tru9I TTAA 3 cut(s) 633, 1569, 1784
TscAI CASTG 3 cut(s) 347, 592, 1439
TseFI GTSAC 3 cut(s) 319, 349, 1760
TseI GCWGC 3 cut(s) 909, 1427, 1479
Tsp45I GTSAC 3 cut(s) 319, 349, 1760
TspGWI ACGGA 1 cut(s) 342
TspRI CASTG 3 cut(s) 347, 592, 1439
VspI ATTAAT 1 cut(s) 1784
XagI CCTNNNNNAGG 1 cut(s) 182
XapI RAATTY 3 cut(s) 356, 388, 1514
XceI RCATGY 4 cut(s) 406, 946, 1007, 1095
XmiI GTMKAC 3 cut(s) 575, 1113, 1438
XmnI GAANNNNTTC 1 cut(s) 443
XspI CTAG 4 cut(s) 528, 924, 1595, 1713
ZrmI AGTACT 1 cut(s) 1711
Zsp2I ATGCAT 1 cut(s) 948
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.