pycom15g09150

Group 2 truncated hemoglobin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0016598
Physical Location & Seq
Reverse (-)
5910437 .. 5911752
1316 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 477 bp
ATGCAGAGCCTGCAAGCCAAGGCCGCCGAGTGGAGCGGCGTCGACTCGGCCGACGCGTTCGCCATCGACGAGACAAACCTGTTTCAGAAACTCGGCCTCCAGGCCTTCATCAACCTCTCCACCAATTTCTACACCAGGGTATACGATGACGAACAGGAGTGGTTCCGGTCGATTTTTGCCAATTCGAAGAAGGAAGACGCCATCCAGAACCAGTACGAGTTTTTCGTGCAGAGAATGGGAGGCCCAAATCTCTTCTCTCAGAGAAGAGGCCATCCTGCTCTGATTGCACGCCACCGTCCATTTCCCGTCACGCACGAAGCTGCGGAGAGGTGGCTGCATCATATGCAGCAAGCACTGGACACTACTCCAGACATTGATGCAGAGTCCAAGGTCAAAATGATGAACTTTTTCAGGCACACTGCGTTCTTCCTTGTGGCCGGAGACGAGTTGAAGAAGAGACAGAATCCGGCACAATAA

Protein Analysis

159

Amino Acids

18.34

Weight (kDa)

6.29

Isoelectric Point (pI)

39.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bac_globin PF01152 26 - 142 3.1e-43 Bacterial-like globin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016598)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32690
fragaria_vesca FvH4_2g36290
malus_domestica MD15G1099500.v1.1
prunus_persica Prupe.1G454300_v2.0.a1
pyrus_communis pycom15g09150
rosa_chinensis RchiOBHm_Chr6g0305591
rosa_laevigata RLG00000010874
rosa_multiflora Rmu_sc0001809.1_g000009
rosa_roxburghii Rroxscaffold_7G00162550
rosa_rugosa Rorug06G0342000
rosa_samantha Rh6BG447500 Rh6CG467700 Rh6DG454300
rosa_wichuraiana Rw6G039570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 36
AccI GTMKAC 2 cut(s) 42, 141
AccII CGCG 1 cut(s) 56
AciI CCGC 3 cut(s) 24, 36, 323
AcoI YGGCCR 2 cut(s) 48, 435
AcyI GRCGYC 2 cut(s) 39, 198
AfaI GTAC 1 cut(s) 215
AfiI CCNNNNNNNGG 1 cut(s) 30
AflIII ACRYGT 1 cut(s) 54
AgsI TTSAA 1 cut(s) 451
AjnI CCWGG 2 cut(s) 99, 134
AluBI AGCT 1 cut(s) 320
AluI AGCT 1 cut(s) 320
Alw26I GTCTC 3 cut(s) 65, 435, 451
AlwNI CAGNNNCTG 1 cut(s) 10
AoxI GGCC 7 cut(s) 21, 48, 94, 102, 241, 268, 435
ApeKI GCWGC 3 cut(s) 320, 334, 346
Asp700I GAANNNNTTC 1 cut(s) 407
AspS9I GGNCC 1 cut(s) 242
AsuII TTCGAA 1 cut(s) 185
BbsI GAAGAC 1 cut(s) 201
BbvI GCAGC 3 cut(s) 307, 321, 358
BccI CCATC 3 cut(s) 71, 209, 279
BciT130I CCWGG 2 cut(s) 101, 136
BcoDI GTCTC 3 cut(s) 65, 435, 451
BisI GCNGC 5 cut(s) 24, 37, 321, 335, 347
BlsI GCNGC 5 cut(s) 25, 38, 322, 336, 348
Bme1390I CCNGG 2 cut(s) 101, 136
BmgT120I GGNCC 1 cut(s) 242
BmiI GGNNCC 1 cut(s) 164
BmrFI CCNGG 2 cut(s) 101, 136
BmsI GCATC 2 cut(s) 346, 367
BpiI GAAGAC 1 cut(s) 201
BpmI CTGGAG 2 cut(s) 83, 351
Bpu14I TTCGAA 1 cut(s) 185
BsaHI GRCGYC 2 cut(s) 39, 198
BsaJI CCNNGG 3 cut(s) 18, 135, 387
BsaWI WCCGGW 1 cut(s) 165
BsaXI ACNNNNNCTCC 2 cut(s) 81, 111
Bsc4I CCNNNNNNNGG 1 cut(s) 30
Bse1I ACTGG 2 cut(s) 211, 360
BseBI CCWGG 2 cut(s) 101, 136
BseDI CCNNGG 3 cut(s) 18, 135, 387
BseGI GGATG 2 cut(s) 201, 271
BseLI CCNNNNNNNGG 1 cut(s) 30
BseMII CTCAG 1 cut(s) 272
BseNI ACTGG 2 cut(s) 211, 360
BseX3I CGGCCG 1 cut(s) 48
BseXI GCAGC 3 cut(s) 307, 321, 358
BsgI GTGCAG 1 cut(s) 248
Bsh1236I CGCG 1 cut(s) 56
Bsh1285I CGRYCG 2 cut(s) 51, 170
BshFI GGCC 7 cut(s) 23, 50, 96, 104, 243, 270, 437
BsiEI CGRYCG 2 cut(s) 51, 170
BsiSI CCGG 3 cut(s) 166, 438, 467
BslI CCNNNNNNNGG 1 cut(s) 30
BsmAI GTCTC 3 cut(s) 65, 435, 451
BsmBI CGTCTC 1 cut(s) 435
BsnI GGCC 7 cut(s) 23, 50, 96, 104, 243, 270, 437
Bsp119I TTCGAA 1 cut(s) 185
BspACI CCGC 3 cut(s) 24, 36, 323
BspANI GGCC 7 cut(s) 23, 50, 96, 104, 243, 270, 437
BspCNI CTCAG 1 cut(s) 271
BspFNI CGCG 1 cut(s) 56
BspLI GGNNCC 1 cut(s) 164
BspT104I TTCGAA 1 cut(s) 185
BsrBI CCGCTC 1 cut(s) 36
BsrI ACTGG 2 cut(s) 211, 360
BssECI CCNNGG 3 cut(s) 18, 135, 387
BssNAI GTATAC 1 cut(s) 142
BssNI GRCGYC 2 cut(s) 39, 198
BssT1I CCWWGG 2 cut(s) 18, 387
Bst1107I GTATAC 1 cut(s) 142
Bst2UI CCWGG 2 cut(s) 101, 136
Bst4CI ACNGT 1 cut(s) 296
Bst6I CTCTTC 3 cut(s) 257, 259, 449
BstACI GRCGYC 2 cut(s) 39, 198
BstAPI GCANNNNNTGC 2 cut(s) 10, 343
BstBI TTCGAA 1 cut(s) 185
BstC8I GCNNGC 4 cut(s) 11, 15, 289, 351
BstDEI CTNAG 1 cut(s) 258
BstF5I GGATG 2 cut(s) 201, 271
BstFNI CGCG 1 cut(s) 56
BstMAI GTCTC 3 cut(s) 65, 435, 451
BstMCI CGRYCG 2 cut(s) 51, 170
BstMWI GCNNNNNNNGC 4 cut(s) 10, 23, 284, 343
BstNI CCWGG 2 cut(s) 101, 136
BstSCI CCNGG 2 cut(s) 99, 134
BstUI CGCG 1 cut(s) 56
BstV1I GCAGC 3 cut(s) 307, 321, 358
BstV2I GAAGAC 1 cut(s) 201
BstZ17I GTATAC 1 cut(s) 142
BstZI CGGCCG 1 cut(s) 48
BsuRI GGCC 7 cut(s) 23, 50, 96, 104, 243, 270, 437
BtsCI GGATG 2 cut(s) 201, 271
BtsI GCAGTG 1 cut(s) 417
BtsIMutI CAGTG 2 cut(s) 353, 417
Cac8I GCNNGC 4 cut(s) 11, 15, 289, 351
CaiI CAGNNNCTG 1 cut(s) 10
Cfr13I GGNCC 1 cut(s) 242
CseI GACGC 3 cut(s) 28, 62, 206
Csp6I GTAC 1 cut(s) 214
CviQI GTAC 1 cut(s) 214
DdeI CTNAG 1 cut(s) 258
EaeI YGGCCR 2 cut(s) 48, 435
EagI CGGCCG 1 cut(s) 48
Eam1104I CTCTTC 3 cut(s) 257, 259, 449
EarI CTCTTC 3 cut(s) 257, 259, 449
EclXI CGGCCG 1 cut(s) 48
Eco130I CCWWGG 2 cut(s) 18, 387
Eco147I AGGCCT 1 cut(s) 104
Eco52I CGGCCG 1 cut(s) 48
EcoRII CCWGG 2 cut(s) 99, 134
EcoT14I CCWWGG 2 cut(s) 18, 387
ErhI CCWWGG 2 cut(s) 18, 387
Esp3I CGTCTC 1 cut(s) 435
FaiI YATR 3 cut(s) 142, 342, 344
FauNDI CATATG 1 cut(s) 342
FblI GTMKAC 2 cut(s) 42, 141
Fnu4HI GCNGC 5 cut(s) 24, 37, 321, 335, 347
FokI GGATG 2 cut(s) 188, 258
Fsp4HI GCNGC 5 cut(s) 24, 37, 321, 335, 347
GluI GCNGC 5 cut(s) 24, 37, 321, 335, 347
GsuI CTGGAG 2 cut(s) 83, 351
HaeIII GGCC 7 cut(s) 23, 50, 96, 104, 243, 270, 437
HapII CCGG 3 cut(s) 166, 438, 467
HgaI GACGC 3 cut(s) 28, 62, 206
Hin1I GRCGYC 2 cut(s) 39, 198
HincII GTYRAC 1 cut(s) 43
HindII GTYRAC 1 cut(s) 43
HinfI GANTC 3 cut(s) 44, 383, 463
HpaII CCGG 3 cut(s) 166, 438, 467
Hpy166II GTNNAC 2 cut(s) 43, 142
Hpy188I TCNGA 3 cut(s) 87, 261, 282
Hpy188III TCNNGA 2 cut(s) 205, 368
Hpy8I GTNNAC 2 cut(s) 43, 142
Hpy99I CGWCG 3 cut(s) 44, 56, 71
HpyAV CCTTC 2 cut(s) 115, 184
HpyCH4III ACNGT 1 cut(s) 296
HpyCH4V TGCA 7 cut(s) 4, 13, 229, 287, 337, 346, 380
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 23, 284, 343
HpyF3I CTNAG 1 cut(s) 258
Hsp92I GRCGYC 2 cut(s) 39, 198
LmnI GCTCC 1 cut(s) 33
Lsp1109I GCAGC 3 cut(s) 307, 321, 358
LweI GCATC 2 cut(s) 346, 367
MaeIII GTNAC 1 cut(s) 307
MbiI CCGCTC 1 cut(s) 36
MboII GAAGA 7 cut(s) 199, 206, 244, 276, 418, 463, 466
MluCI AATT 2 cut(s) 124, 181
MluI ACGCGT 1 cut(s) 54
MlyI GAGTC 2 cut(s) 38, 392
MnlI CCTC 5 cut(s) 107, 125, 233, 260, 321
MroXI GAANNNNTTC 1 cut(s) 407
MspI CCGG 3 cut(s) 166, 438, 467
MspR9I CCNGG 2 cut(s) 101, 136
MvaI CCWGG 2 cut(s) 101, 136
MvnI CGCG 1 cut(s) 56
MwoI GCNNNNNNNGC 4 cut(s) 10, 23, 284, 343
NdeI CATATG 1 cut(s) 342
NlaIV GGNNCC 1 cut(s) 164
NmeAIII GCCGAG 3 cut(s) 26, 52, 72
NmuCI GTSAC 1 cut(s) 307
NspV TTCGAA 1 cut(s) 185
PceI AGGCCT 1 cut(s) 104
PcsI WCGNNNNNNNCGW 4 cut(s) 48, 53, 66, 222
PdmI GAANNNNTTC 1 cut(s) 407
PfeI GAWTC 1 cut(s) 463
PkrI GCNGC 5 cut(s) 25, 38, 322, 336, 348
PleI GAGTC 2 cut(s) 38, 391
PpsI GAGTC 2 cut(s) 38, 391
Psp6I CCWGG 2 cut(s) 99, 134
PspGI CCWGG 2 cut(s) 99, 134
PspN4I GGNNCC 1 cut(s) 164
PspPI GGNCC 1 cut(s) 242
PstNI CAGNNNCTG 1 cut(s) 10
RsaI GTAC 1 cut(s) 215
RsaNI GTAC 1 cut(s) 214
SalI GTCGAC 1 cut(s) 41
SatI GCNGC 5 cut(s) 24, 37, 321, 335, 347
Sau96I GGNCC 1 cut(s) 242
SchI GAGTC 2 cut(s) 38, 392
ScrFI CCNGG 2 cut(s) 101, 136
SetI ASST 5 cut(s) 81, 117, 322, 332, 393
SfaNI GCATC 2 cut(s) 346, 367
SfuI TTCGAA 1 cut(s) 185
Sse9I AATT 2 cut(s) 124, 181
SseBI AGGCCT 1 cut(s) 104
SsiI CCGC 3 cut(s) 24, 36, 323
StuI AGGCCT 1 cut(s) 104
StyD4I CCNGG 2 cut(s) 99, 134
StyI CCWWGG 2 cut(s) 18, 387
TaaI ACNGT 1 cut(s) 296
TaqI TCGA 4 cut(s) 42, 66, 170, 185
TasI AATT 2 cut(s) 124, 181
TauI GCSGC 2 cut(s) 26, 39
TfiI GAWTC 1 cut(s) 463
TscAI CASTG 2 cut(s) 360, 424
TseFI GTSAC 1 cut(s) 307
TseI GCWGC 3 cut(s) 320, 334, 346
Tsp45I GTSAC 1 cut(s) 307
TspDTI ATGAA 2 cut(s) 97, 416
TspRI CASTG 2 cut(s) 360, 424
XmiI GTMKAC 2 cut(s) 42, 141
XmnI GAANNNNTTC 1 cut(s) 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.