pycom15g10730

1-deoxy-D-xylulose-5-phosphate synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0007296
Physical Location & Seq
Forward (+)
7170040 .. 7172286
2247 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1002 bp
ATGTCGTGGAACAAACATATGCACATAAAATACTTACAGGAAGGCGGGGCCTCGTACATACAGCTCGACGAAATAATGGTTTTTCTAGTCATTCATCTCGACCTGAGAGTGAATATCGATCCATTCGGGCCAGGGCATGGGTGCAGTAGCGTTTCTGCTGGACTGGGCATGGCAGTTGCACGGGATATTAAGGGAAAGCGGGAACGTATTGTTTCAGTCATCAGCAATGGGACAACGATGGCCGGTCAGGTCTATGAGGCAATGAGTAATGCAGGTTATTTGGACTCAAATATGGTAGTTATTTTAAATGACAGCCGGCACTCTTTACACCCAAAGACTGAGGAGGGCCTGAAGACAGCTATTAGTGCTCTGTCGAGTACCCTAAGCAAGCTCCAGTCAAGTAAATCCTTTCGGAGGTTTAGAGAAGTTGCTAAGGGTGTTACTAAAAGAATTGGAGGAGGCATGCATGAATTGGCAGCTAAAGTTGATGAGTATGCACGTGGTATGGTCGGTCCACTAGGATCAACTCTTTTCGAAGAGCTCGGATTGTATTACATTGGCCCTGTTGACGGACACAATATTGAAGACTTGATTTGCGTTCTACAAGAAGTGGCATCTCTGAATTCAATGGGTCCTGTTTTGGTTCATGTGCTAACGGAAGAAAATCGGGAAGGTGAAAACAACCCAAAGAGTGGGGTACAAGGTTTGAGTAATTCAGATGATTTACCATCCAAAATTCGCCATAGAACTTATAGCGACTGCTTTGTGGAGGCTTTAGTGATGGAGGCAGAGGAGGACAAAGATATTGTGACTGTTCATGCAGGAATGCATATGGAAACAGCATTTCAACTGTTTCGTGAAAGATTTCCTGACAAATTTTTTGATGTGGGAATGGCTGAGCAACATGCAGTTACTTTTTCTGCTGGTTTGGCATGTGGAGGACTAAAACCATTTTGCATTATCCCGTCTGCATTTCTACAAAGAGCTTATGATCAGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00730 ko00900 ko01100 ko01110 ko01130 map00730 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
DXP_synthase_N Transket_pyr Transketolase_C

Protein Analysis

334

Amino Acids

36.51

Weight (kDa)

5.81

Isoelectric Point (pI)

51.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DXP_synthase_N PF13292 39 - 219 8.1e-42 1-deoxy-D-xylulose-5-phosphate synthase
Transket_pyr PF02779 250 - 332 4.9e-12 Transketolase, pyrimidine binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 263
AccB7I CCANNNNNTGG 2 cut(s) 137, 692
AciI CCGC 2 cut(s) 45, 199
AclWI GGATC 2 cut(s) 113, 529
AcoI YGGCCR 1 cut(s) 240
AcsI RAATTY 3 cut(s) 622, 735, 875
AcuI CTGAAG 1 cut(s) 371
AcvI CACGTG 1 cut(s) 500
AfaI GTAC 3 cut(s) 56, 379, 699
AfiI CCNNNNNNNGG 4 cut(s) 137, 414, 569, 692
AgsI TTSAA 3 cut(s) 584, 627, 848
AjnI CCWGG 1 cut(s) 130
AluBI AGCT 6 cut(s) 64, 359, 391, 479, 541, 986
AluI AGCT 6 cut(s) 64, 359, 391, 479, 541, 986
Alw21I GWGCWC 2 cut(s) 370, 543
AlwI GGATC 2 cut(s) 113, 529
AoxI GGCC 5 cut(s) 48, 128, 240, 346, 559
ApeKI GCWGC 1 cut(s) 476
ApoI RAATTY 3 cut(s) 622, 735, 875
ArsI GACNNNNNNTTYG 2 cut(s) 863, 895
Asp700I GAANNNNTTC 1 cut(s) 864
AspS9I GGNCC 6 cut(s) 48, 128, 346, 512, 560, 632
AsuHPI GGTGA 1 cut(s) 686
AsuII TTCGAA 1 cut(s) 534
AvaII GGWCC 2 cut(s) 512, 632
BanII GRGCYC 1 cut(s) 543
BbrPI CACGTG 1 cut(s) 500
BbsI GAAGAC 2 cut(s) 359, 591
Bbv12I GWGCWC 2 cut(s) 370, 543
BbvI GCAGC 1 cut(s) 488
BccI CCATC 3 cut(s) 232, 736, 775
BciT130I CCWGG 1 cut(s) 132
BclI TGATCA 1 cut(s) 991
BfaI CTAG 2 cut(s) 86, 518
BfuAI ACCTGC 1 cut(s) 263
BisI GCNGC 1 cut(s) 477
BlpI GCTNAGC 1 cut(s) 897
BlsI GCNGC 1 cut(s) 478
Bme1390I CCNGG 1 cut(s) 132
Bme18I GGWCC 2 cut(s) 512, 632
BmgT120I GGNCC 6 cut(s) 48, 128, 346, 512, 560, 632
BmiI GGNNCC 2 cut(s) 49, 633
BmrFI CCNGG 1 cut(s) 132
BmrI ACTGGG 1 cut(s) 173
BmsI GCATC 1 cut(s) 623
BmuI ACTGGG 1 cut(s) 173
BpiI GAAGAC 2 cut(s) 359, 591
BpmI CTGGAG 1 cut(s) 377
Bpu10I CCTNAGC 2 cut(s) 383, 432
Bpu1102I GCTNAGC 1 cut(s) 897
Bpu14I TTCGAA 1 cut(s) 534
Bsa29I ATCGAT 1 cut(s) 117
BsaAI YACGTR 1 cut(s) 500
BsaJI CCNNGG 1 cut(s) 131
Bsc4I CCNNNNNNNGG 4 cut(s) 137, 414, 569, 692
Bse118I RCCGGY 2 cut(s) 242, 315
Bse1I ACTGG 2 cut(s) 168, 394
Bse3DI GCAATG 2 cut(s) 232, 267
BseBI CCWGG 1 cut(s) 132
BseCI ATCGAT 1 cut(s) 117
BseDI CCNNGG 1 cut(s) 131
BseGI GGATG 1 cut(s) 728
BseLI CCNNNNNNNGG 4 cut(s) 137, 414, 569, 692
BseMI GCAATG 2 cut(s) 232, 267
BseMII CTCAG 3 cut(s) 95, 330, 888
BseNI ACTGG 2 cut(s) 168, 394
BseRI GAGGAG 3 cut(s) 356, 471, 806
BseXI GCAGC 1 cut(s) 488
BsgI GTGCAG 1 cut(s) 163
BshFI GGCC 5 cut(s) 50, 130, 242, 348, 561
BshVI ATCGAT 1 cut(s) 117
BsiHKAI GWGCWC 2 cut(s) 370, 543
BsiSI CCGG 2 cut(s) 243, 316
BslFI GGGAC 1 cut(s) 244
BslI CCNNNNNNNGG 4 cut(s) 137, 414, 569, 692
BsmFI GGGAC 1 cut(s) 244
BsmI GAATGC 1 cut(s) 831
BsnI GGCC 5 cut(s) 50, 130, 242, 348, 561
Bsp119I TTCGAA 1 cut(s) 534
Bsp1286I GDGCHC 2 cut(s) 370, 543
Bsp143I GATC 3 cut(s) 118, 521, 991
Bsp1720I GCTNAGC 1 cut(s) 897
BspACI CCGC 2 cut(s) 45, 199
BspANI GGCC 5 cut(s) 50, 130, 242, 348, 561
BspCNI CTCAG 3 cut(s) 96, 331, 889
BspDI ATCGAT 1 cut(s) 117
BspHI TCATGA 1 cut(s) 998
BspLI GGNNCC 2 cut(s) 49, 633
BspMI ACCTGC 1 cut(s) 263
BspPI GGATC 2 cut(s) 113, 529
BspQI GCTCTTC 1 cut(s) 531
BspT104I TTCGAA 1 cut(s) 534
BsrDI GCAATG 2 cut(s) 232, 267
BsrFI RCCGGY 2 cut(s) 242, 315
BsrI ACTGG 2 cut(s) 168, 394
BssAI RCCGGY 2 cut(s) 242, 315
BssECI CCNNGG 1 cut(s) 131
BssMI GATC 3 cut(s) 118, 521, 991
Bst2UI CCWGG 1 cut(s) 132
Bst4CI ACNGT 2 cut(s) 814, 852
Bst6I CTCTTC 1 cut(s) 531
BstBAI YACGTR 1 cut(s) 500
BstBI TTCGAA 1 cut(s) 534
BstC8I GCNNGC 3 cut(s) 317, 389, 464
BstDEI CTNAG 5 cut(s) 104, 339, 383, 432, 897
BstENI CCTNNNNNAGG 1 cut(s) 412
BstF5I GGATG 1 cut(s) 728
BstKTI GATC 3 cut(s) 121, 524, 994
BstMBI GATC 3 cut(s) 118, 521, 991
BstMWI GCNNNNNNNGC 2 cut(s) 365, 929
BstNI CCWGG 1 cut(s) 132
BstNSI RCATGY 3 cut(s) 466, 908, 936
BstSCI CCNGG 1 cut(s) 130
BstV1I GCAGC 1 cut(s) 488
BstV2I GAAGAC 2 cut(s) 359, 591
Bsu15I ATCGAT 1 cut(s) 117
BsuRI GGCC 5 cut(s) 50, 130, 242, 348, 561
BsuTUI ATCGAT 1 cut(s) 117
BtsCI GGATG 1 cut(s) 728
BveI ACCTGC 1 cut(s) 263
Cac8I GCNNGC 3 cut(s) 317, 389, 464
CciI TCATGA 1 cut(s) 998
Cfr10I RCCGGY 2 cut(s) 242, 315
Cfr13I GGNCC 6 cut(s) 48, 128, 346, 512, 560, 632
ClaI ATCGAT 1 cut(s) 117
Csp6I GTAC 3 cut(s) 55, 378, 698
CviAII CATG 9 cut(s) 137, 169, 463, 467, 647, 818, 905, 933, 999
CviQI GTAC 3 cut(s) 55, 378, 698
DdeI CTNAG 5 cut(s) 104, 339, 383, 432, 897
DpnI GATC 3 cut(s) 120, 523, 993
DpnII GATC 3 cut(s) 118, 521, 991
DraI TTTAAA 1 cut(s) 306
EaeI YGGCCR 1 cut(s) 240
Eam1104I CTCTTC 1 cut(s) 531
EarI CTCTTC 1 cut(s) 531
Ecl136II GAGCTC 1 cut(s) 541
Eco24I GRGCYC 1 cut(s) 543
Eco47I GGWCC 2 cut(s) 512, 632
Eco53kI GAGCTC 1 cut(s) 541
Eco57I CTGAAG 1 cut(s) 371
Eco72I CACGTG 1 cut(s) 500
EcoICRI GAGCTC 1 cut(s) 541
EcoNI CCTNNNNNAGG 1 cut(s) 412
EcoO109I RGGNCCY 3 cut(s) 48, 346, 632
EcoRI GAATTC 1 cut(s) 622
EcoRII CCWGG 1 cut(s) 130
EcoT22I ATGCAT 2 cut(s) 468, 831
EcoT38I GRGCYC 1 cut(s) 543
FaeI CATG 9 cut(s) 140, 172, 466, 470, 650, 821, 908, 936, 1002
FaqI GGGAC 1 cut(s) 244
FatI CATG 9 cut(s) 136, 168, 462, 466, 646, 817, 904, 932, 998
FauI CCCGC 2 cut(s) 38, 192
FauNDI CATATG 2 cut(s) 18, 831
FbaI TGATCA 1 cut(s) 991
Fnu4HI GCNGC 1 cut(s) 477
FokI GGATG 1 cut(s) 715
FriOI GRGCYC 1 cut(s) 543
Fsp4HI GCNGC 1 cut(s) 477
FspBI CTAG 2 cut(s) 86, 518
GluI GCNGC 1 cut(s) 477
GsuI CTGGAG 1 cut(s) 377
HaeIII GGCC 5 cut(s) 50, 130, 242, 348, 561
HapII CCGG 2 cut(s) 243, 316
Hin1II CATG 9 cut(s) 140, 172, 466, 470, 650, 821, 908, 936, 1002
HincII GTYRAC 1 cut(s) 568
HindII GTYRAC 1 cut(s) 568
HinfI GANTC 1 cut(s) 284
HpaII CCGG 2 cut(s) 243, 316
HphI GGTGA 1 cut(s) 686
Hpy166II GTNNAC 2 cut(s) 515, 568
Hpy188I TCNGA 4 cut(s) 414, 545, 621, 718
Hpy188III TCNNGA 5 cut(s) 98, 668, 857, 869, 999
Hpy8I GTNNAC 2 cut(s) 515, 568
Hpy99I CGWCG 1 cut(s) 71
HpyAV CCTTC 2 cut(s) 35, 665
HpyCH4III ACNGT 2 cut(s) 814, 852
HpyCH4IV ACGT 2 cut(s) 205, 499
HpyF10VI GCNNNNNNNGC 2 cut(s) 365, 929
HpyF3I CTNAG 5 cut(s) 104, 339, 383, 432, 897
HpySE526I ACGT 2 cut(s) 205, 499
Hsp92II CATG 9 cut(s) 140, 172, 466, 470, 650, 821, 908, 936, 1002
KroI GCCGGC 1 cut(s) 315
KroNI GCCGGC 1 cut(s) 317
Ksp22I TGATCA 1 cut(s) 991
Kzo9I GATC 3 cut(s) 118, 521, 991
LguI GCTCTTC 1 cut(s) 531
LmnI GCTCC 1 cut(s) 396
Lsp1109I GCAGC 1 cut(s) 488
LweI GCATC 1 cut(s) 623
MaeI CTAG 2 cut(s) 86, 518
MaeII ACGT 2 cut(s) 205, 499
MaeIII GTNAC 3 cut(s) 439, 808, 910
MalI GATC 3 cut(s) 120, 523, 993
MboI GATC 3 cut(s) 118, 521, 991
MboII GAAGA 4 cut(s) 364, 548, 596, 671
MhlI GDGCHC 2 cut(s) 370, 543
MluCI AATT 6 cut(s) 450, 470, 622, 712, 735, 875
MlyI GAGTC 1 cut(s) 278
Mph1103I ATGCAT 2 cut(s) 468, 831
MroNI GCCGGC 1 cut(s) 315
MroXI GAANNNNTTC 1 cut(s) 864
MseI TTAA 2 cut(s) 189, 305
MspI CCGG 2 cut(s) 243, 316
MspR9I CCNGG 1 cut(s) 132
Mva1269I GAATGC 1 cut(s) 831
MvaI CCWGG 1 cut(s) 132
MwoI GCNNNNNNNGC 2 cut(s) 365, 929
NaeI GCCGGC 1 cut(s) 317
NdeI CATATG 2 cut(s) 18, 831
NdeII GATC 3 cut(s) 118, 521, 991
NgoMIV GCCGGC 1 cut(s) 315
NlaIII CATG 9 cut(s) 140, 172, 466, 470, 650, 821, 908, 936, 1002
NlaIV GGNNCC 2 cut(s) 49, 633
NmuCI GTSAC 1 cut(s) 808
NsiI ATGCAT 2 cut(s) 468, 831
NspI RCATGY 3 cut(s) 466, 908, 936
NspV TTCGAA 1 cut(s) 534
PaeI GCATGC 1 cut(s) 466
PagI TCATGA 1 cut(s) 998
PciSI GCTCTTC 1 cut(s) 531
PctI GAATGC 1 cut(s) 831
PdiI GCCGGC 1 cut(s) 317
PdmI GAANNNNTTC 1 cut(s) 864
PflMI CCANNNNNTGG 2 cut(s) 137, 692
PkrI GCNGC 1 cut(s) 478
PleI GAGTC 1 cut(s) 278
PmaCI CACGTG 1 cut(s) 500
PmlI CACGTG 1 cut(s) 500
PpsI GAGTC 1 cut(s) 278
Ppu21I YACGTR 1 cut(s) 500
PpuMI RGGWCCY 1 cut(s) 632
Psp124BI GAGCTC 1 cut(s) 543
Psp5II RGGWCCY 1 cut(s) 632
Psp6I CCWGG 1 cut(s) 130
PspCI CACGTG 1 cut(s) 500
PspGI CCWGG 1 cut(s) 130
PspN4I GGNNCC 2 cut(s) 49, 633
PspPI GGNCC 6 cut(s) 48, 128, 346, 512, 560, 632
PspPPI RGGWCCY 1 cut(s) 632
RsaI GTAC 3 cut(s) 56, 379, 699
RsaNI GTAC 3 cut(s) 55, 378, 698
SacI GAGCTC 1 cut(s) 543
SapI GCTCTTC 1 cut(s) 531
SaqAI TTAA 2 cut(s) 189, 305
SatI GCNGC 1 cut(s) 477
Sau3AI GATC 3 cut(s) 118, 521, 991
Sau96I GGNCC 6 cut(s) 48, 128, 346, 512, 560, 632
SchI GAGTC 1 cut(s) 278
ScrFI CCNGG 1 cut(s) 132
SduI GDGCHC 2 cut(s) 370, 543
SfaNI GCATC 1 cut(s) 623
SfuI TTCGAA 1 cut(s) 534
SinI GGWCC 2 cut(s) 512, 632
SphI GCATGC 1 cut(s) 466
Sse9I AATT 6 cut(s) 450, 470, 622, 712, 735, 875
SsiI CCGC 2 cut(s) 45, 199
SspI AATATT 1 cut(s) 580
SspMI CTAG 2 cut(s) 86, 518
SstI GAGCTC 1 cut(s) 543
StyD4I CCNGG 1 cut(s) 130
TaaI ACNGT 2 cut(s) 814, 852
TaiI ACGT 2 cut(s) 208, 502
TaqI TCGA 5 cut(s) 66, 99, 117, 374, 534
TaqII GACCGA 1 cut(s) 500
TasI AATT 6 cut(s) 450, 470, 622, 712, 735, 875
Tru1I TTAA 2 cut(s) 189, 305
Tru9I TTAA 2 cut(s) 189, 305
TseFI GTSAC 1 cut(s) 808
TseI GCWGC 1 cut(s) 476
Tsp45I GTSAC 1 cut(s) 808
TspDTI ATGAA 4 cut(s) 83, 483, 635, 806
TspGWI ACGGA 2 cut(s) 585, 671
Van91I CCANNNNNTGG 2 cut(s) 137, 692
VpaK11BI GGWCC 2 cut(s) 512, 632
XagI CCTNNNNNAGG 1 cut(s) 412
XapI RAATTY 3 cut(s) 622, 735, 875
XceI RCATGY 3 cut(s) 466, 908, 936
XmnI GAANNNNTTC 1 cut(s) 864
XspI CTAG 2 cut(s) 86, 518
Zsp2I ATGCAT 2 cut(s) 468, 831
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.