pycom15g12170

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0011127
Physical Location & Seq
Forward (+)
8286472 .. 8288765
2294 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g12170.1

Sequence Viewer

Length: 1995 bp
ATGAGTGAAGGCAAACCCACTTCAGCTTCAGTGAATGGTCCGTCGGTGACGGTCATGTCGTCGGAATCCTGTGGCTCCAGCTGGCTTCTGGGTCTGCCAGATGATGTGTTTTCCATTGTCATCTGCTCCCTTTCACCTCGGGACATTTGTAATCTCAGTCTTTGCTGCCGGAGTTTTTTTTATGCACTCGTTGACTCCGATAAGGTCTGGCTCACCCAATGCCAGATGCTTGGAATTGTACCTCTCCAAGACCTTCTTGTCTGGCGTCAAGCCGTCTCATCTTTCAAGGCGCTTTGCCGCTTTCTCTGTAGTGTTCAGCCTCTCATGGGAATTTGGGTTCACCAGAATCCAGAGCTTGGCAATGTAGTCTATGTCATGCCAGGTTTTGTTTCCGTTGTTGGCTGCCGCATTATACCTCAAGAACTCGGCCCGTTAGGCATTGAGGATGGCCCTCTTCTCTGGGCTCCTGTATTTGAAATTATTGGCGATTCTGATGGTTCCTCGTCCTTTTTTCTACATGGAAGGGACAAGGGACAGGACTATGTCTATCCTGGTTTTGTGAAACCTGTTGGCAGGTCATGCAATGTTCTGTTGCTTGAGATCGAGCCTAGACCCCATAAAAATGGGGTTGAATTGTTGCACGGCAAGAGTTTTGTTAATGACTCAGATAAGGAGCTGTCAAGGAAGATTTGTAGGTCGAATAGTGAACTTTCAAGGTCACAGAGGGTGTTTCAGCAGAGTGAGACTTCAGTGCCCTTCAGTCGTTTGGCTTTCAGTGACAGGAGAAAATTGCTTGAGGTTTTCATGAACCAAATCCGTCTAAAGGTTCCCGATGCAGCAATTGGGCCACTCTTTCCAAGGTTGAGGGGTAGTGGGGAGAACACTCAGATTGGTACAGCGCTCTTATTGGAACGAAGATCATTGCTTTTTCAAATGCGTAAACTTGGTGGATATCAGATGGATTTGAAGGAAAGTAATCTGCTTCCTCCGAATCCCACTCAACTGCGGTTAAGTGAGATCAGATCTTGTCTTGATCAGTCAAGTGGTTCGCAAAGTTCTATCAATGAGGATGATGGCCACAGACAATGCAACAGGAGGAGAAGTCTTGGCAGGTACTTTAGGGATAGCATCAATCAGATGCTGGGAAAGTCAATCTCTGTCAATGGTAATTCAAAGAATAGTTCTTCCAGCAGTGAGAGTAAGTATGCATCCCTTCAGGATTTTCTGAAATCAGGTGATACAATAGAACTGACTTTACATGCTTCCACTGTGAAGTTATCTTCTTATCGAGCATGGCCAAACATGCATGAAAGTCGATTTGCCCTATACAAATTGCCTTTGCGGATCCCAACTGCTGAGCAAGAGTATGCTGGTTTATGGGGAGGCACTTTTGGGTGGCCTCCCGGGAGGCCGACCAAAGACAAGCCTGGAAAGGCTCTCTTCTTCCTTTTGCTTTCTTATGAAGAGTGCGAGGGGCAACAGTTTCTAATTGCAACCAAAATATTAGAGGGTACCCACTATGTTCTACACCCTAATGGCTCAGCCATGTTTATAGTAAATATCGATGAACCTTCATTGGATCCATTCCCTTGGGATACTGATGCTGATTCCCATCCCGTGAATGTTAATCATGCTTTTAAAGGTGAGGGTATTGCCAATGGGTATGGTTTTAGATACCCTGGCTCGAAACCCGGTTCCCTCTTTGTGTTTGAGGATGGTCAACTTGCCTTCATTTGGAAGGAGTCCAGGGCTGTCTTGACTTTGCAGAGGTTGGACTTGCAAGAGCTTTTGAAAAAAGGTGAAAGGGTGCCTGCGCTACCTCCAATTGCTAACTTTTCATACTTAACCAAGTCTTACTCAAATGTGTTTGCTGAGTTCCCAAGCATCTCAACTTCGTGGGCGTCAACAAGGTATGCCTTGCCTCCTCTGGATGTGCTCATGATGCTTTTGTCTTCCCCATGCAGGAGCAGTCTGACACCCCTAGGTGAACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

665

Amino Acids

73.87

Weight (kDa)

7.84

Isoelectric Point (pI)

50.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 29 - 65 6.6e-06 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011127)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G39450
fragaria_vesca FvH4_1g24840 FvH4_1g24840 FvH4_1g24840
malus_domestica MD15G1134900.v1.1
prunus_persica Prupe.4G275200_v2.0.a1 Prupe.4G275200_v2.0.a1
pyrus_communis pycom15g12170
rosa_chinensis RchiOBHm_Chr2g0122451
rosa_laevigata RLG00000018678
rosa_multiflora Rmu_co8054258.1_g000001 Rmu_sc0003832.1_g000018
rosa_roxburghii Rroxscaffold_2G00121630
rosa_rugosa Rorug02G0238000 Rorug02G0238100
rosa_samantha Rh2AG297400 Rh2BG306200 Rh2CG284500 Rh2DG321000
rosa_wichuraiana Rw2G023660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 55, 257
Acc36I ACCTGC 2 cut(s) 564, 1101
Acc65I GGTACC 1 cut(s) 1511
AccB1I GGYRCC 2 cut(s) 1511, 1807
AccB7I CCANNNNNTGG 1 cut(s) 356
AciI CCGC 4 cut(s) 298, 406, 1006, 1342
AclWI GGATC 4 cut(s) 1339, 1352, 1574, 1587
AcoI YGGCCR 2 cut(s) 1075, 1295
AcsI RAATTY 1 cut(s) 330
AcuI CTGAAG 5 cut(s) 6, 12, 732, 742, 1199
AcyI GRCGYC 2 cut(s) 265, 1901
AfaI GTAC 4 cut(s) 240, 895, 1115, 1513
AfeI AGCGCT 1 cut(s) 900
AfiI CCNNNNNNNGG 5 cut(s) 326, 356, 823, 1734, 1962
AgsI TTSAA 8 cut(s) 286, 476, 632, 714, 932, 967, 1173, 1792
AjnI CCWGG 5 cut(s) 379, 550, 1426, 1678, 1745
AloI GAACNNNNNNTCC 2 cut(s) 321, 353
AluBI AGCT 5 cut(s) 26, 81, 355, 676, 1786
AluI AGCT 5 cut(s) 26, 81, 355, 676, 1786
Alw21I GWGCWC 1 cut(s) 1938
Alw26I GTCTC 2 cut(s) 280, 737
AlwI GGATC 4 cut(s) 1339, 1352, 1574, 1587
AlwNI CAGNNNCTG 1 cut(s) 1141
Ama87I CYCGRG 2 cut(s) 138, 1403
Aor51HI AGCGCT 1 cut(s) 900
AoxI GGCC 7 cut(s) 427, 448, 845, 1075, 1295, 1397, 1409
ApeKI GCWGC 3 cut(s) 165, 402, 836
ApoI RAATTY 1 cut(s) 330
Asp718I GGTACC 1 cut(s) 1511
AspA2I CCTAGG 1 cut(s) 1981
AspLEI GCGC 3 cut(s) 292, 901, 1816
AspS9I GGNCC 4 cut(s) 38, 428, 449, 845
AsuC2I CCSGG 3 cut(s) 1404, 1405, 1692
AsuHPI GGTGA 7 cut(s) 58, 126, 205, 332, 1247, 1655, 1811
AvaI CYCGRG 2 cut(s) 138, 1403
AvaII GGWCC 1 cut(s) 38
AvrII CCTAGG 1 cut(s) 1981
BaeGI GKGCMC 1 cut(s) 756
BalI TGGCCA 2 cut(s) 1077, 1297
BamHI GGATCC 2 cut(s) 1344, 1579
BanI GGYRCC 2 cut(s) 1511, 1807
BanII GRGCYC 1 cut(s) 466
BbsI GAAGAC 1 cut(s) 1944
Bbv12I GWGCWC 1 cut(s) 1938
BbvI GCAGC 3 cut(s) 152, 389, 848
BccI CCATC 6 cut(s) 440, 488, 952, 1067, 1620, 1709
BceAI ACGGC 2 cut(s) 257, 658
BcgI CGANNNNNNTGC 2 cut(s) 1295, 1329
BciT130I CCWGG 5 cut(s) 381, 552, 1428, 1680, 1747
BciVI GTATCC 1 cut(s) 1588
BclI TGATCA 1 cut(s) 1033
BcnI CCSGG 3 cut(s) 1404, 1405, 1692
BcoDI GTCTC 2 cut(s) 280, 737
BfaI CTAG 2 cut(s) 609, 1982
BfmI CTRYAG 1 cut(s) 307
BfoI RGCGCY 2 cut(s) 293, 902
BfuAI ACCTGC 2 cut(s) 564, 1101
BfuI GTATCC 1 cut(s) 1588
BglI GCCNNNNNGGC 1 cut(s) 435
BglII AGATCT 1 cut(s) 1022
BisI GCNGC 5 cut(s) 166, 298, 403, 406, 837
BlnI CCTAGG 1 cut(s) 1981
BlpI GCTNAGC 2 cut(s) 1356, 1540
BlsI GCNGC 5 cut(s) 167, 299, 404, 407, 838
Bme1390I CCNGG 8 cut(s) 381, 552, 1404, 1405, 1428, 1680, 1692, 1747
Bme18I GGWCC 1 cut(s) 38
BmeT110I CYCGRG 2 cut(s) 138, 1403
BmgT120I GGNCC 4 cut(s) 38, 428, 449, 845
BmiI GGNNCC 9 cut(s) 76, 465, 499, 828, 1346, 1513, 1581, 1696, 1809
BmrFI CCNGG 8 cut(s) 381, 552, 1404, 1405, 1428, 1680, 1692, 1747
BmsI GCATC 8 cut(s) 216, 823, 1128, 1137, 1217, 1591, 1893, 1932
BpiI GAAGAC 1 cut(s) 1944
BpmI CTGGAG 1 cut(s) 61
Bpu1102I GCTNAGC 2 cut(s) 1356, 1540
BpuEI CTTGAG 3 cut(s) 402, 617, 815
BpuMI CCSGG 3 cut(s) 1404, 1405, 1692
Bsa29I ATCGAT 1 cut(s) 1563
BsaBI GATNNNNATC 1 cut(s) 1611
BsaHI GRCGYC 2 cut(s) 265, 1901
BsaJI CCNNGG 7 cut(s) 137, 857, 1403, 1589, 1678, 1746, 1981
BsaXI ACNNNNNCTCC 2 cut(s) 1087, 1117
Bsc4I CCNNNNNNNGG 5 cut(s) 326, 356, 823, 1734, 1962
Bse3DI GCAATG 3 cut(s) 367, 589, 920
Bse8I GATNNNNATC 1 cut(s) 1611
BseBI CCWGG 5 cut(s) 381, 552, 1428, 1680, 1747
BseCI ATCGAT 1 cut(s) 1563
BseDI CCNNGG 7 cut(s) 137, 857, 1403, 1589, 1678, 1746, 1981
BseGI GGATG 6 cut(s) 451, 1075, 1208, 1612, 1720, 1936
BseJI GATNNNNATC 1 cut(s) 1611
BseLI CCNNNNNNNGG 5 cut(s) 326, 356, 823, 1734, 1962
BseMI GCAATG 3 cut(s) 367, 589, 920
BseMII CTCAG 6 cut(s) 169, 678, 899, 1347, 1554, 1863
BseRI GAGGAG 2 cut(s) 1111, 1914
BseSI GKGCMC 1 cut(s) 756
BseXI GCAGC 3 cut(s) 152, 389, 848
BseYI CCCAGC 1 cut(s) 1141
BshFI GGCC 7 cut(s) 429, 450, 847, 1077, 1297, 1399, 1411
BshNI GGYRCC 2 cut(s) 1511, 1807
BshVI ATCGAT 1 cut(s) 1563
BsiHKAI GWGCWC 1 cut(s) 1938
BsiHKCI CYCGRG 2 cut(s) 138, 1403
BsiSI CCGG 3 cut(s) 169, 1404, 1692
BslFI GGGAC 3 cut(s) 155, 539, 546
BslI CCNNNNNNNGG 5 cut(s) 326, 356, 823, 1734, 1962
BsmAI GTCTC 2 cut(s) 280, 737
BsmBI CGTCTC 1 cut(s) 280
BsmFI GGGAC 3 cut(s) 155, 539, 546
BsnI GGCC 7 cut(s) 429, 450, 847, 1077, 1297, 1399, 1411
BsoBI CYCGRG 2 cut(s) 138, 1403
Bsp1286I GDGCHC 3 cut(s) 466, 756, 1938
Bsp143I GATC 7 cut(s) 600, 917, 1017, 1022, 1033, 1344, 1579
Bsp1720I GCTNAGC 2 cut(s) 1356, 1540
BspACI CCGC 4 cut(s) 298, 406, 1006, 1342
BspANI GGCC 7 cut(s) 429, 450, 847, 1077, 1297, 1399, 1411
BspCNI CTCAG 6 cut(s) 168, 677, 898, 1348, 1553, 1864
BspDI ATCGAT 1 cut(s) 1563
BspHI TCATGA 2 cut(s) 804, 1938
BspLI GGNNCC 9 cut(s) 76, 465, 499, 828, 1346, 1513, 1581, 1696, 1809
BspMI ACCTGC 2 cut(s) 564, 1101
BspPI GGATC 4 cut(s) 1339, 1352, 1574, 1587
BspT107I GGYRCC 2 cut(s) 1511, 1807
BsrDI GCAATG 3 cut(s) 367, 589, 920
BssECI CCNNGG 7 cut(s) 137, 857, 1403, 1589, 1678, 1746, 1981
BssMI GATC 7 cut(s) 600, 917, 1017, 1022, 1033, 1344, 1579
BssNI GRCGYC 2 cut(s) 265, 1901
BssT1I CCWWGG 3 cut(s) 857, 1589, 1981
Bst2UI CCWGG 5 cut(s) 381, 552, 1428, 1680, 1747
Bst4CI ACNGT 3 cut(s) 52, 1270, 1482
Bst6I CTCTTC 3 cut(s) 459, 1445, 1458
BstACI GRCGYC 2 cut(s) 265, 1901
BstAPI GCANNNNNTGC 1 cut(s) 579
BstC8I GCNNGC 2 cut(s) 83, 1812
BstDEI CTNAG 6 cut(s) 155, 664, 885, 1356, 1540, 1872
BstF5I GGATG 6 cut(s) 451, 1075, 1208, 1612, 1720, 1936
BstH2I RGCGCY 2 cut(s) 293, 902
BstHHI GCGC 3 cut(s) 292, 901, 1816
BstKTI GATC 7 cut(s) 603, 920, 1020, 1025, 1036, 1347, 1582
BstMAI GTCTC 2 cut(s) 280, 737
BstMBI GATC 7 cut(s) 600, 917, 1017, 1022, 1033, 1344, 1579
BstMWI GCNNNNNNNGC 4 cut(s) 435, 579, 1303, 1942
BstNI CCWGG 5 cut(s) 381, 552, 1428, 1680, 1747
BstNSI RCATGY 2 cut(s) 1262, 1306
BstSCI CCNGG 8 cut(s) 379, 550, 1402, 1403, 1426, 1678, 1690, 1745
BstSFI CTRYAG 1 cut(s) 307
BstSLI GKGCMC 1 cut(s) 756
BstV1I GCAGC 3 cut(s) 152, 389, 848
BstV2I GAAGAC 1 cut(s) 1944
BstX2I RGATCY 3 cut(s) 1022, 1344, 1579
BstXI CCANNNNNNTGG 3 cut(s) 230, 623, 1590
BstYI RGATCY 3 cut(s) 1022, 1344, 1579
Bsu15I ATCGAT 1 cut(s) 1563
BsuI GTATCC 1 cut(s) 1588
BsuRI GGCC 7 cut(s) 429, 450, 847, 1077, 1297, 1399, 1411
BsuTUI ATCGAT 1 cut(s) 1563
BtsCI GGATG 6 cut(s) 451, 1075, 1208, 1612, 1720, 1936
BtsI GCAGTG 1 cut(s) 1198
BtsIMutI CAGTG 5 cut(s) 36, 756, 781, 1198, 1266
BveI ACCTGC 2 cut(s) 564, 1101
Cac8I GCNNGC 2 cut(s) 83, 1812
CaiI CAGNNNCTG 1 cut(s) 1141
CciI TCATGA 2 cut(s) 804, 1938
CfoI GCGC 3 cut(s) 292, 901, 1816
Cfr13I GGNCC 4 cut(s) 38, 428, 449, 845
Cfr9I CCCGGG 1 cut(s) 1403
ClaI ATCGAT 1 cut(s) 1563
CseI GACGC 2 cut(s) 254, 1890
Csp6I GTAC 4 cut(s) 239, 894, 1114, 1512
CviQI GTAC 4 cut(s) 239, 894, 1114, 1512
DdeI CTNAG 6 cut(s) 155, 664, 885, 1356, 1540, 1872
DpnI GATC 7 cut(s) 602, 919, 1019, 1024, 1035, 1346, 1581
DpnII GATC 7 cut(s) 600, 917, 1017, 1022, 1033, 1344, 1579
DraI TTTAAA 1 cut(s) 1639
DrdI GACNNNNNNGTC 2 cut(s) 55, 257
DseDI GACNNNNNNGTC 2 cut(s) 55, 257
EaeI YGGCCR 2 cut(s) 1075, 1295
Eam1104I CTCTTC 3 cut(s) 459, 1445, 1458
EarI CTCTTC 3 cut(s) 459, 1445, 1458
Eco130I CCWWGG 3 cut(s) 857, 1589, 1981
Eco24I GRGCYC 1 cut(s) 466
Eco32I GATATC 1 cut(s) 953
Eco47I GGWCC 1 cut(s) 38
Eco47III AGCGCT 1 cut(s) 900
Eco57I CTGAAG 5 cut(s) 6, 12, 732, 742, 1199
Eco88I CYCGRG 2 cut(s) 138, 1403
EcoRII CCWGG 5 cut(s) 379, 550, 1426, 1678, 1745
EcoRV GATATC 1 cut(s) 953
EcoT14I CCWWGG 3 cut(s) 857, 1589, 1981
EcoT22I ATGCAT 2 cut(s) 1210, 1308
EcoT38I GRGCYC 1 cut(s) 466
ErhI CCWWGG 3 cut(s) 857, 1589, 1981
Esp3I CGTCTC 1 cut(s) 280
FalI AAGNNNNNCTT 4 cut(s) 240, 272, 1424, 1456
FaqI GGGAC 3 cut(s) 155, 539, 546
FbaI TGATCA 1 cut(s) 1033
Fnu4HI GCNGC 5 cut(s) 166, 298, 403, 406, 837
FokI GGATG 6 cut(s) 458, 1082, 1195, 1599, 1727, 1943
FriOI GRGCYC 1 cut(s) 466
Fsp4HI GCNGC 5 cut(s) 166, 298, 403, 406, 837
FspBI CTAG 2 cut(s) 609, 1982
GlaI GCGC 3 cut(s) 291, 900, 1815
GluI GCNGC 5 cut(s) 166, 298, 403, 406, 837
GsaI CCCAGC 1 cut(s) 1145
GsuI CTGGAG 1 cut(s) 61
HaeII RGCGCY 2 cut(s) 293, 902
HaeIII GGCC 7 cut(s) 429, 450, 847, 1077, 1297, 1399, 1411
HapII CCGG 3 cut(s) 169, 1404, 1692
HgaI GACGC 2 cut(s) 254, 1890
HhaI GCGC 3 cut(s) 292, 901, 1816
Hin1I GRCGYC 2 cut(s) 265, 1901
Hin6I GCGC 3 cut(s) 290, 899, 1814
HinP1I GCGC 3 cut(s) 290, 899, 1814
HincII GTYRAC 3 cut(s) 193, 1721, 1905
HindII GTYRAC 3 cut(s) 193, 1721, 1905
HinfI GANTC 8 cut(s) 65, 194, 346, 488, 662, 991, 1607, 1742
HpaII CCGG 3 cut(s) 169, 1404, 1692
HphI GGTGA 7 cut(s) 58, 126, 205, 332, 1247, 1655, 1811
Hpy166II GTNNAC 7 cut(s) 193, 340, 707, 941, 1721, 1905, 1988
Hpy8I GTNNAC 7 cut(s) 193, 340, 707, 941, 1721, 1905, 1988
Hpy99I CGWCG 2 cut(s) 46, 64
HpyAV CCTTC 8 cut(s) 263, 516, 766, 961, 1223, 1581, 1732, 1738
HpyCH4III ACNGT 3 cut(s) 52, 1270, 1482
HpyF10VI GCNNNNNNNGC 4 cut(s) 435, 579, 1303, 1942
HpyF3I CTNAG 6 cut(s) 155, 664, 885, 1356, 1540, 1872
Hsp92I GRCGYC 2 cut(s) 265, 1901
HspAI GCGC 3 cut(s) 290, 899, 1814
KpnI GGTACC 1 cut(s) 1515
Ksp22I TGATCA 1 cut(s) 1033
Kzo9I GATC 7 cut(s) 600, 917, 1017, 1022, 1033, 1344, 1579
LmnI GCTCC 5 cut(s) 80, 131, 469, 673, 1965
Lsp1109I GCAGC 3 cut(s) 152, 389, 848
LweI GCATC 8 cut(s) 216, 823, 1128, 1137, 1217, 1591, 1893, 1932
MaeI CTAG 2 cut(s) 609, 1982
MaeIII GTNAC 3 cut(s) 46, 717, 776
MalI GATC 7 cut(s) 602, 919, 1019, 1024, 1035, 1346, 1581
MboI GATC 7 cut(s) 600, 917, 1017, 1022, 1033, 1344, 1579
MboII GAAGA 9 cut(s) 446, 697, 927, 1176, 1272, 1432, 1435, 1475, 1944
MfeI CAATTG 2 cut(s) 840, 1824
MflI RGATCY 3 cut(s) 1022, 1344, 1579
MhlI GDGCHC 3 cut(s) 466, 756, 1938
MlsI TGGCCA 2 cut(s) 1077, 1297
MluNI TGGCCA 2 cut(s) 1077, 1297
MlyI GAGTC 3 cut(s) 188, 656, 1751
MmeI TCCRAC 2 cut(s) 42, 1752
Mox20I TGGCCA 2 cut(s) 1077, 1297
Mph1103I ATGCAT 2 cut(s) 1210, 1308
MscI TGGCCA 2 cut(s) 1077, 1297
MseI TTAA 5 cut(s) 657, 1010, 1626, 1638, 1844
MslI CAYNNNNRTG 2 cut(s) 621, 1533
Msp20I TGGCCA 2 cut(s) 1077, 1297
MspA1I CMGCKG 1 cut(s) 81
MspI CCGG 3 cut(s) 169, 1404, 1692
MspR9I CCNGG 8 cut(s) 381, 552, 1404, 1405, 1428, 1680, 1692, 1747
MunI CAATTG 2 cut(s) 840, 1824
MvaI CCWGG 5 cut(s) 381, 552, 1428, 1680, 1747
MwoI GCNNNNNNNGC 4 cut(s) 435, 579, 1303, 1942
NciI CCSGG 3 cut(s) 1404, 1405, 1692
NdeII GATC 7 cut(s) 600, 917, 1017, 1022, 1033, 1344, 1579
NlaIV GGNNCC 9 cut(s) 76, 465, 499, 828, 1346, 1513, 1581, 1696, 1809
NmeAIII GCCGAG 1 cut(s) 405
NmuCI GTSAC 3 cut(s) 46, 717, 776
NsiI ATGCAT 2 cut(s) 1210, 1308
NspI RCATGY 2 cut(s) 1262, 1306
PagI TCATGA 2 cut(s) 804, 1938
PcsI WCGNNNNNNNCGW 2 cut(s) 56, 195
PfeI GAWTC 5 cut(s) 65, 346, 488, 991, 1607
PflFI GACNNNGTC 1 cut(s) 542
PflMI CCANNNNNTGG 1 cut(s) 356
PkrI GCNGC 5 cut(s) 167, 299, 404, 407, 838
PleI GAGTC 3 cut(s) 188, 656, 1750
PpsI GAGTC 3 cut(s) 188, 656, 1750
Psp6I CCWGG 5 cut(s) 379, 550, 1426, 1678, 1745
PspFI CCCAGC 1 cut(s) 1141
PspGI CCWGG 5 cut(s) 379, 550, 1426, 1678, 1745
PspN4I GGNNCC 9 cut(s) 76, 465, 499, 828, 1346, 1513, 1581, 1696, 1809
PspPI GGNCC 4 cut(s) 38, 428, 449, 845
PsrI GAACNNNNNNTAC 2 cut(s) 1239, 1271
PstNI CAGNNNCTG 1 cut(s) 1141
PsuI RGATCY 3 cut(s) 1022, 1344, 1579
PsyI GACNNNGTC 1 cut(s) 542
PvuII CAGCTG 1 cut(s) 81
RsaI GTAC 4 cut(s) 240, 895, 1115, 1513
RsaNI GTAC 4 cut(s) 239, 894, 1114, 1512
RseI CAYNNNNRTG 2 cut(s) 621, 1533
SaqAI TTAA 5 cut(s) 657, 1010, 1626, 1638, 1844
SatI GCNGC 5 cut(s) 166, 298, 403, 406, 837
Sau3AI GATC 7 cut(s) 600, 917, 1017, 1022, 1033, 1344, 1579
Sau96I GGNCC 4 cut(s) 38, 428, 449, 845
SchI GAGTC 3 cut(s) 188, 656, 1751
ScrFI CCNGG 8 cut(s) 381, 552, 1404, 1405, 1428, 1680, 1692, 1747
SduI GDGCHC 3 cut(s) 466, 756, 1938
SfaNI GCATC 8 cut(s) 216, 823, 1128, 1137, 1217, 1591, 1893, 1932
SfcI CTRYAG 1 cut(s) 307
SinI GGWCC 1 cut(s) 38
SmaI CCCGGG 1 cut(s) 1405
SmiMI CAYNNNNRTG 2 cut(s) 621, 1533
SmlI CTYRAG 3 cut(s) 417, 596, 794
SmoI CTYRAG 3 cut(s) 417, 596, 794
SsiI CCGC 4 cut(s) 298, 406, 1006, 1342
SspI AATATT 1 cut(s) 1503
SspMI CTAG 2 cut(s) 609, 1982
StyD4I CCNGG 8 cut(s) 379, 550, 1402, 1403, 1426, 1678, 1690, 1745
StyI CCWWGG 3 cut(s) 857, 1589, 1981
TaaI ACNGT 3 cut(s) 52, 1270, 1482
TaqI TCGA 6 cut(s) 603, 698, 1288, 1315, 1563, 1685
TauI GCSGC 2 cut(s) 300, 408
TfiI GAWTC 5 cut(s) 65, 346, 488, 991, 1607
Tru1I TTAA 5 cut(s) 657, 1010, 1626, 1638, 1844
Tru9I TTAA 5 cut(s) 657, 1010, 1626, 1638, 1844
TscAI CASTG 5 cut(s) 36, 756, 781, 1198, 1273
TseFI GTSAC 3 cut(s) 46, 717, 776
TseI GCWGC 3 cut(s) 165, 402, 836
Tsp45I GTSAC 3 cut(s) 46, 717, 776
TspDTI ATGAA 8 cut(s) 793, 821, 1323, 1476, 1563, 1581, 1720, 1827
TspGWI ACGGA 3 cut(s) 30, 382, 806
TspMI CCCGGG 1 cut(s) 1403
TspRI CASTG 5 cut(s) 36, 756, 781, 1198, 1273
Tth111I GACNNNGTC 1 cut(s) 542
Van91I CCANNNNNTGG 1 cut(s) 356
VpaK11BI GGWCC 1 cut(s) 38
XapI RAATTY 1 cut(s) 330
XceI RCATGY 2 cut(s) 1262, 1306
XcmI CCANNNNNNNNNTGG 1 cut(s) 85
XmaI CCCGGG 1 cut(s) 1403
XmaJI CCTAGG 1 cut(s) 1981
XspI CTAG 2 cut(s) 609, 1982
Zsp2I ATGCAT 2 cut(s) 1210, 1308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.